##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211194_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6043919 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.31451182585339 31.0 31.0 34.0 28.0 34.0 2 31.31846058823753 31.0 31.0 34.0 28.0 34.0 3 31.380457944588603 31.0 31.0 34.0 28.0 34.0 4 34.9754106565624 37.0 35.0 37.0 32.0 37.0 5 35.03467650708092 37.0 35.0 37.0 32.0 37.0 6 35.097138793554315 37.0 35.0 37.0 32.0 37.0 7 35.01677620100468 37.0 35.0 37.0 32.0 37.0 8 35.01595570688489 37.0 35.0 37.0 32.0 37.0 9 36.56779152731862 39.0 37.0 39.0 32.0 39.0 10-11 36.509559277680594 39.0 37.0 39.0 32.0 39.0 12-13 36.40187186492737 39.0 37.0 39.0 32.0 39.0 14-15 37.57830465629999 40.0 37.0 41.0 32.0 41.0 16-17 37.547201244755264 40.0 37.0 41.0 32.0 41.0 18-19 37.55833681755166 40.0 37.0 41.0 32.0 41.0 20-21 37.52216235856238 40.0 37.0 41.0 31.5 41.0 22-23 37.41508282953494 40.0 37.0 41.0 31.0 41.0 24-25 37.32222883860621 40.0 37.0 41.0 31.0 41.0 26-27 37.17306982439705 40.0 36.0 41.0 31.0 41.0 28-29 37.039402083317135 40.0 36.0 41.0 30.0 41.0 30-31 36.87878849799277 39.0 36.0 41.0 30.0 41.0 32-33 36.677731203876164 39.0 35.5 41.0 30.0 41.0 34-35 36.51476508867839 39.0 35.0 41.0 30.0 41.0 36-37 36.399247822480746 39.0 35.0 41.0 29.0 41.0 38-39 36.25418201335921 39.0 35.0 40.5 29.0 41.0 40-41 36.0851341654314 39.0 35.0 40.0 28.0 41.0 42-43 35.88103257174691 38.5 35.0 40.0 27.0 41.0 44-45 35.67265742641488 38.0 34.5 40.0 26.5 41.0 46-47 35.442354373048346 38.0 34.0 40.0 26.0 41.0 48-49 35.24841944440354 38.0 34.0 40.0 25.0 41.0 50-51 34.57787778095636 37.5 33.5 39.5 24.0 40.5 52-53 34.697107208088 38.0 33.5 39.5 24.5 40.5 54-55 35.19694837075083 38.0 34.0 40.0 26.0 41.0 56-57 35.170926761262024 38.0 34.0 40.0 26.0 41.0 58-59 34.97145461413365 38.0 34.0 40.0 26.0 41.0 60-61 34.74669746566755 37.0 33.5 40.0 25.5 41.0 62-63 34.444041192477926 37.0 33.0 40.0 25.0 41.0 64-65 34.195182215380456 36.0 33.0 40.0 25.0 41.0 66-67 34.01763607354764 36.0 33.0 39.0 24.5 41.0 68-69 33.71543943590243 35.5 33.0 39.0 24.0 41.0 70-71 33.39285776331549 35.0 32.0 39.0 23.5 40.5 72-73 33.04209900893774 35.0 32.0 37.5 23.0 40.0 74-75 32.70890741586709 35.0 32.0 37.0 22.5 39.5 76-77 32.36641730307768 35.0 31.0 36.5 22.0 39.0 78-79 32.02163447259965 35.0 31.0 36.0 21.0 39.0 80-81 31.719865785759207 35.0 31.0 36.0 20.0 37.5 82-83 31.42546566226318 35.0 31.0 35.0 20.0 37.0 84-85 31.173478582356914 34.0 30.5 35.0 20.0 37.0 86-87 30.93955809136423 34.0 30.0 35.0 19.0 36.0 88-89 30.718509298354263 34.0 30.0 35.0 19.0 36.0 90-91 30.493153200762617 34.0 30.0 35.0 18.0 36.0 92-93 30.296828101104595 34.0 29.0 35.0 18.0 35.0 94-95 30.116126804479016 34.0 29.0 35.0 17.5 35.0 96-97 29.928171026117326 34.0 29.0 35.0 17.0 35.0 98-99 29.690101902424573 34.0 29.0 35.0 15.0 35.0 100 28.55686980583294 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5499239592306608 1101 2 -0.48101048411076874 1101 3 -0.5529638024521226 1101 4 -0.5000791725789284 1101 5 -0.35275601093634634 1101 6 -0.43085559653555094 1101 7 -0.4761070602434785 1101 8 -0.46641569897223434 1101 9 -0.5533186200900602 1101 10-11 -0.5847284910478336 1101 12-13 -0.6636933117342707 1101 14-15 -0.8203944602810793 1101 16-17 -0.7124705425685889 1101 18-19 -0.7340616951548284 1101 20-21 -0.8152512072583988 1101 22-23 -0.8760365601381892 1101 24-25 -0.9942221018526425 1101 26-27 -0.9407225039950404 1101 28-29 -0.9533772545223727 1101 30-31 -0.9787354493870879 1101 32-33 -0.9321226132294171 1101 34-35 -0.932049900343209 1101 36-37 -1.096573953956515 1101 38-39 -1.0447300788121368 1101 40-41 -1.120829466168729 1101 42-43 -1.195568022438536 1101 44-45 -1.1143021860496631 1101 46-47 -1.2330059383590282 1101 48-49 -1.1811759168456604 1101 50-51 -1.326601809109988 1101 52-53 -1.1966954300253505 1101 54-55 -1.0422565911201147 1101 56-57 -1.06373683079309 1101 58-59 -0.976338218626239 1101 60-61 -0.9481532323807613 1101 62-63 -0.9668887227892142 1101 64-65 -0.8822368641673322 1101 66-67 -0.9752650089073782 1101 68-69 -1.0397170735596504 1101 70-71 -1.0977693950388598 1101 72-73 -1.0465964126846146 1101 74-75 -1.1393086201963492 1101 76-77 -1.1706921619392787 1101 78-79 -1.1843911577175597 1101 80-81 -1.2823300900677914 1101 82-83 -1.3172185872346702 1101 84-85 -1.2199220132698194 1101 86-87 -1.2941038486428162 1101 88-89 -1.3120604332581607 1101 90-91 -1.5148665304075628 1101 92-93 -1.7136712919767838 1101 94-95 -1.6219232012824456 1101 96-97 -1.6825779466222777 1101 98-99 -1.8042192952854776 1101 100 -1.9036427090575643 1102 1 -0.382504313438357 1102 2 -0.4002913474144911 1102 3 -0.4024918428719637 1102 4 -0.2932715433369779 1102 5 -0.34012265395112706 1102 6 -0.34755777420067346 1102 7 -0.33778676198426894 1102 8 -0.3763575843358993 1102 9 -0.5341851415281837 1102 10-11 -0.5818779911903036 1102 12-13 -0.5285012488156227 1102 14-15 -0.6726360153476705 1102 16-17 -0.7676956849525922 1102 18-19 -0.6733685390414479 1102 20-21 -0.814816334363222 1102 22-23 -0.7751340400680959 1102 24-25 -0.7646142222258021 1102 26-27 -0.8443158322397366 1102 28-29 -0.9099400884227862 1102 30-31 -0.9717775991675381 1102 32-33 -0.9866927012588818 1102 34-35 -0.9465238416394328 1102 36-37 -1.0413383456967367 1102 38-39 -1.045385630751909 1102 40-41 -1.0911183541951317 1102 42-43 -1.1204583961217196 1102 44-45 -1.2183710067295621 1102 46-47 -1.1251186652952043 1102 48-49 -1.1500246439729125 1102 50-51 -1.1609910348640682 1102 52-53 -0.9979879739762225 1102 54-55 -0.8275515214896956 1102 56-57 -0.946910555374366 1102 58-59 -0.9628419327681712 1102 60-61 -0.9027514173889202 1102 62-63 -0.9652270867091133 1102 64-65 -0.8822764387784119 1102 66-67 -0.9274506685677082 1102 68-69 -0.9925647537676028 1102 70-71 -1.0154879236860026 1102 72-73 -0.995404016052138 1102 74-75 -0.9793163128638369 1102 76-77 -1.0774677632793086 1102 78-79 -1.1274342197375766 1102 80-81 -1.1641093652453556 1102 82-83 -1.0875722442704046 1102 84-85 -1.0846748179089083 1102 86-87 -1.1496048854445498 1102 88-89 -1.2539304049521967 1102 90-91 -1.2483156849936883 1102 92-93 -1.2869685968230336 1102 94-95 -1.307480160028316 1102 96-97 -1.3650845255281787 1102 98-99 -1.5529518462158123 1102 100 -1.4258685802380562 1103 1 -0.4402467340483902 1103 2 -0.3503149066358162 1103 3 -0.41549421537630593 1103 4 -0.3352773196438079 1103 5 -0.27495975887799773 1103 6 -0.31514454965218164 1103 7 -0.2738158332214695 1103 8 -0.201112062804782 1103 9 -0.4462129379200448 1103 10-11 -0.44326833129813537 1103 12-13 -0.4715534137238251 1103 14-15 -0.5597190156096232 1103 16-17 -0.5727289551700281 1103 18-19 -0.5583791633395307 1103 20-21 -0.6779354177678556 1103 22-23 -0.6772157123127869 1103 24-25 -0.6331574966211235 1103 26-27 -0.6621255823801064 1103 28-29 -0.7138479292434425 1103 30-31 -0.7517350001771135 1103 32-33 -0.7931221422473342 1103 34-35 -0.8771731065049764 1103 36-37 -0.7709549935174138 1103 38-39 -0.830690265937065 1103 40-41 -0.7972929125473627 1103 42-43 -0.9425420665546014 1103 44-45 -0.898435948805961 1103 46-47 -0.9054625630215156 1103 48-49 -1.0890197858018524 1103 50-51 -1.051266334425037 1103 52-53 -0.8639611020265079 1103 54-55 -0.7776825129370835 1103 56-57 -0.6702366398068165 1103 58-59 -0.6328660752478896 1103 60-61 -0.6885343436615656 1103 62-63 -0.5821665045692228 1103 64-65 -0.5807328272008405 1103 66-67 -0.7047148211388858 1103 68-69 -0.6215830943629044 1103 70-71 -0.5668844802014519 1103 72-73 -0.6715054420612816 1103 74-75 -0.6632962888542053 1103 76-77 -0.6780503206074151 1103 78-79 -0.6763302079602376 1103 80-81 -0.8103051296178023 1103 82-83 -0.711905566644969 1103 84-85 -0.8440514568225694 1103 86-87 -0.8673473752004774 1103 88-89 -0.9497463006896716 1103 90-91 -0.9320738179189156 1103 92-93 -0.9655828197037337 1103 94-95 -1.0073615620214298 1103 96-97 -1.0972521125021046 1103 98-99 -1.1286694613138692 1103 100 -1.3172319992257542 1104 1 -0.5815580046024103 1104 2 -0.5658672484757297 1104 3 -0.6201610205345851 1104 4 -0.4628415504560621 1104 5 -0.5420837141984762 1104 6 -0.5388767920977315 1104 7 -0.6126220461683047 1104 8 -0.5216718672120138 1104 9 -0.650478745938706 1104 10-11 -0.692361046106619 1104 12-13 -0.6443485526681982 1104 14-15 -0.8633187855004891 1104 16-17 -0.9782728277151307 1104 18-19 -0.8583960220301421 1104 20-21 -0.8882732307722208 1104 22-23 -0.8765715824928861 1104 24-25 -0.9833550708872565 1104 26-27 -0.9272333501616217 1104 28-29 -0.947261838062694 1104 30-31 -0.9569980756497145 1104 32-33 -0.9804759829926226 1104 34-35 -1.0847342851751378 1104 36-37 -1.0961217267665901 1104 38-39 -1.0710021437235469 1104 40-41 -1.121022710083281 1104 42-43 -1.0404280986100858 1104 44-45 -1.083105630328511 1104 46-47 -1.1176259085527676 1104 48-49 -1.1471729362197252 1104 50-51 -1.181204061138466 1104 52-53 -0.9798724460876116 1104 54-55 -0.8601737959553546 1104 56-57 -0.9642948702035881 1104 58-59 -1.0214143901778243 1104 60-61 -0.8888780212530847 1104 62-63 -1.033663188760407 1104 64-65 -0.9707228717844956 1104 66-67 -0.9615403856253764 1104 68-69 -1.0043290954175745 1104 70-71 -1.053482923768783 1104 72-73 -1.0158737764865649 1104 74-75 -1.0665490573548198 1104 76-77 -1.0059736654994715 1104 78-79 -1.0652083459937636 1104 80-81 -1.1205119082496076 1104 82-83 -1.1778766432042005 1104 84-85 -1.1814813774029567 1104 86-87 -1.2212060178761348 1104 88-89 -1.1882568233889792 1104 90-91 -1.1410834537392383 1104 92-93 -1.2024449299963251 1104 94-95 -1.224729129416243 1104 96-97 -1.446020900753755 1104 98-99 -1.3563837264826937 1104 100 -1.3569922702431896 1105 1 -0.48292146375534273 1105 2 -0.46080788289004815 1105 3 -0.5581110471161921 1105 4 -0.36737566351649065 1105 5 -0.26085918823710585 1105 6 -0.3482016193172939 1105 7 -0.3515166057997021 1105 8 -0.4281570701182673 1105 9 -0.6374094565536268 1105 10-11 -0.6379323734147846 1105 12-13 -0.5772213153830705 1105 14-15 -0.7141977505899746 1105 16-17 -0.7533906728329782 1105 18-19 -0.7027456587482064 1105 20-21 -0.7563558109600663 1105 22-23 -0.7398451958191288 1105 24-25 -0.7718473622216493 1105 26-27 -0.8670981383948373 1105 28-29 -0.9040130342349428 1105 30-31 -0.905822105421116 1105 32-33 -0.9371883700207988 1105 34-35 -0.8730194171971064 1105 36-37 -0.9708952514874767 1105 38-39 -0.9726486083700152 1105 40-41 -0.978797120857692 1105 42-43 -1.024679383387685 1105 44-45 -1.0872084125365618 1105 46-47 -1.1216149214891473 1105 48-49 -1.1770290411284634 1105 50-51 -1.1741260884289133 1105 52-53 -1.0606500466546862 1105 54-55 -0.8238147670700045 1105 56-57 -0.8098929563279924 1105 58-59 -0.7701464475940867 1105 60-61 -0.8115930664418229 1105 62-63 -0.8138710569156373 1105 64-65 -0.8065231553216137 1105 66-67 -0.7979786276951941 1105 68-69 -0.8635256220877849 1105 70-71 -0.8576388691878876 1105 72-73 -0.7624550275941289 1105 74-75 -0.790076436408512 1105 76-77 -0.6618384537326811 1105 78-79 -0.8524163913597 1105 80-81 -0.8105154524636795 1105 82-83 -0.9542601271666307 1105 84-85 -0.8531349304775624 1105 86-87 -0.8415249971424821 1105 88-89 -0.9612056094956571 1105 90-91 -1.0624483305777979 1105 92-93 -1.0007972248749368 1105 94-95 -1.0329933505752003 1105 96-97 -1.0358429812074128 1105 98-99 -0.9445589878684828 1105 100 -1.0228994118345405 1106 1 -0.5573593411660447 1106 2 -0.48904004241403953 1106 3 -0.6349894623485426 1106 4 -0.45167103290350497 1106 5 -0.529406684556676 1106 6 -0.5036113492594012 1106 7 -0.49896656089163827 1106 8 -0.5144536431180953 1106 9 -0.6183776418916196 1106 10-11 -0.6432401049837111 1106 12-13 -0.7537842854205152 1106 14-15 -0.9471153091342472 1106 16-17 -0.9670523597552645 1106 18-19 -1.0119407611400746 1106 20-21 -0.886405456264491 1106 22-23 -0.892110695678042 1106 24-25 -0.8430928637686677 1106 26-27 -0.952478451867286 1106 28-29 -0.8837365132712307 1106 30-31 -0.9760058641409586 1106 32-33 -1.0356067288341606 1106 34-35 -1.0120208496212442 1106 36-37 -1.0661373200089699 1106 38-39 -1.0764888349968658 1106 40-41 -1.1250299132181851 1106 42-43 -1.021337514717139 1106 44-45 -1.0883017084410653 1106 46-47 -1.0836032097348607 1106 48-49 -1.111918972869539 1106 50-51 -1.1302603854258706 1106 52-53 -0.9321869775586435 1106 54-55 -0.7776350079681649 1106 56-57 -0.8496783599029456 1106 58-59 -0.9368249526077008 1106 60-61 -0.8331580654800135 1106 62-63 -0.958656131964581 1106 64-65 -0.9782747327298367 1106 66-67 -0.9459495334441144 1106 68-69 -0.9714373050401832 1106 70-71 -0.8983303896636485 1106 72-73 -0.8257338763223458 1106 74-75 -0.8754392380022864 1106 76-77 -0.8383880164856343 1106 78-79 -0.8612280042107017 1106 80-81 -0.772555452257027 1106 82-83 -0.8591082329344459 1106 84-85 -0.8849412723640135 1106 86-87 -0.82749241972326 1106 88-89 -0.8194132273498873 1106 90-91 -0.9787907105318112 1106 92-93 -0.935192592635957 1106 94-95 -1.0823451844397347 1106 96-97 -1.0839383655104236 1106 98-99 -1.005763590224749 1106 100 -1.186555984654337 1107 1 -0.6258296534002277 1107 2 -0.5898093914688154 1107 3 -0.5589913193355756 1107 4 -0.4015921689931403 1107 5 -0.3566843612834205 1107 6 -0.4102730999071582 1107 7 -0.4275977487629845 1107 8 -0.3936306314066442 1107 9 -0.5678009561778765 1107 10-11 -0.5865939966870926 1107 12-13 -0.6202202775936669 1107 14-15 -0.7402876366075049 1107 16-17 -0.7025933885220823 1107 18-19 -0.8411096552217927 1107 20-21 -0.7746251307912502 1107 22-23 -0.8004105090931546 1107 24-25 -0.7530319724922734 1107 26-27 -0.7997932689588083 1107 28-29 -0.9210566106313678 1107 30-31 -0.8505525386326482 1107 32-33 -0.911786520326892 1107 34-35 -0.9323624650901223 1107 36-37 -1.0224651893054428 1107 38-39 -1.035503253918307 1107 40-41 -0.9154423002628107 1107 42-43 -0.8995415287946926 1107 44-45 -0.893077209342664 1107 46-47 -0.8562644925786458 1107 48-49 -0.8059148000781278 1107 50-51 -0.8179241651795763 1107 52-53 -0.8327144177935111 1107 54-55 -0.7752782177024784 1107 56-57 -0.7636975854227757 1107 58-59 -0.7822241979555287 1107 60-61 -0.706016002601821 1107 62-63 -0.7321506594195384 1107 64-65 -0.6668822957520106 1107 66-67 -0.6919001521672783 1107 68-69 -0.6438622426611218 1107 70-71 -0.704114592867306 1107 72-73 -0.701850620995863 1107 74-75 -0.6223341236493098 1107 76-77 -0.585881995132155 1107 78-79 -0.5994936977669028 1107 80-81 -0.6547480942548098 1107 82-83 -0.6825164325843929 1107 84-85 -0.6186574225720243 1107 86-87 -0.6393757825148434 1107 88-89 -0.7670568614527973 1107 90-91 -0.8976949490129833 1107 92-93 -0.7781463827654669 1107 94-95 -0.8340950496091004 1107 96-97 -0.8207072548741614 1107 98-99 -0.8648937182355354 1107 100 -0.9614282278654684 1108 1 -0.6105636953871638 1108 2 -0.5273492435979144 1108 3 -0.5172903145265835 1108 4 -0.37633948615179236 1108 5 -0.34683639619996853 1108 6 -0.44054927707373537 1108 7 -0.3779009583835631 1108 8 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1.2393098261238222 2311 64-65 1.21923719395528 2311 66-67 1.2407220300461361 2311 68-69 1.1724024981091787 2311 70-71 1.1344872812703883 2311 72-73 1.1700399665716574 2311 74-75 1.21887957655683 2311 76-77 1.1959781827688971 2311 78-79 1.3122553049492574 2311 80-81 1.1673491634677085 2311 82-83 1.2167027945617122 2311 84-85 1.2699273490215184 2311 86-87 1.2814278142488753 2311 88-89 1.340706449815439 2311 90-91 1.3713293549129624 2311 92-93 1.3196483250501316 2311 94-95 1.431747364639346 2311 96-97 1.402693621638857 2311 98-99 1.5294141104667673 2311 100 1.5946293284526192 2312 1 0.6370240240780269 2312 2 0.6592018213834976 2312 3 0.6835011291825559 2312 4 0.4306853538986246 2312 5 0.4880636348345462 2312 6 0.5519875678549084 2312 7 0.47972448718257255 2312 8 0.6041968342746316 2312 9 0.8374110054097983 2312 10-11 0.7795948807451154 2312 12-13 0.9355479083237768 2312 14-15 1.1438750047902673 2312 16-17 1.1336821782051416 2312 18-19 1.1848805205300508 2312 20-21 1.1517241379950462 2312 22-23 1.2414232866032862 2312 24-25 1.16918617368583 2312 26-27 1.161235403949668 2312 28-29 1.272836964726416 2312 30-31 1.3004091076156428 2312 32-33 1.3094863903792984 2312 34-35 1.275691842965898 2312 36-37 1.271904980245651 2312 38-39 1.2635213400285892 2312 40-41 1.3520470175841268 2312 42-43 1.4161947141777063 2312 44-45 1.4245559931420857 2312 46-47 1.480062553491706 2312 48-49 1.490723919457153 2312 50-51 1.4351407619111356 2312 52-53 1.2519659962226086 2312 54-55 1.208707667010053 2312 56-57 1.166461609207019 2312 58-59 1.1565865307536356 2312 60-61 1.1612447257538818 2312 62-63 1.1791520987017705 2312 64-65 1.169055183734521 2312 66-67 1.1734225139930956 2312 68-69 1.0848865341048537 2312 70-71 1.117821641420008 2312 72-73 1.119666810278332 2312 74-75 1.1370616517624015 2312 76-77 1.1597196643255572 2312 78-79 1.1377182426130013 2312 80-81 1.1299709229568116 2312 82-83 1.165134276411635 2312 84-85 1.2559298842731401 2312 86-87 1.184001191701558 2312 88-89 1.1435284680200475 2312 90-91 1.2221932203825894 2312 92-93 1.2996845266541186 2312 94-95 1.3463213902522249 2312 96-97 1.3408016225984376 2312 98-99 1.08942458821274 2312 100 1.366399651872321 2313 1 0.6677362214418174 2313 2 0.6647173133588318 2313 3 0.6225471080485434 2313 4 0.46968957157505997 2313 5 0.404816879577119 2313 6 0.5603866190406848 2313 7 0.6032914468701875 2313 8 0.6075847871696425 2313 9 0.7303182442332812 2313 10-11 0.8278532761702095 2313 12-13 0.8525852106405623 2313 14-15 1.006023966342262 2313 16-17 1.0081251043078012 2313 18-19 1.0285393792802537 2313 20-21 1.069398247524255 2313 22-23 1.1210018204555183 2313 24-25 1.088880852536171 2313 26-27 1.117923590870312 2313 28-29 1.1080325415441834 2313 30-31 1.0820425160783813 2313 32-33 1.1420628683587282 2313 34-35 1.1510119781336314 2313 36-37 1.1822326446502842 2313 38-39 1.1801696928857837 2313 40-41 1.1980693763213068 2313 42-43 1.2259002183432344 2313 44-45 1.2205489560760725 2313 46-47 1.3715341037322446 2313 48-49 1.289593494984203 2313 50-51 1.3312924581080523 2313 52-53 1.2246394294242648 2313 54-55 1.127379848598295 2313 56-57 1.046458610549891 2313 58-59 1.1208580811571522 2313 60-61 1.0565490364864658 2313 62-63 1.053481006571296 2313 64-65 1.0659291040549874 2313 66-67 1.0960114673639723 2313 68-69 1.09551775142927 2313 70-71 1.0652937449453859 2313 72-73 1.1371429106551147 2313 74-75 1.071863104807342 2313 76-77 0.9065613979730927 2313 78-79 1.011538173252756 2313 80-81 1.0705837612835616 2313 82-83 1.070625404297843 2313 84-85 1.1662340198124461 2313 86-87 1.2100212036635902 2313 88-89 1.2466901974955427 2313 90-91 1.2845334074401222 2313 92-93 1.285067662141266 2313 94-95 1.3232876089129952 2313 96-97 1.386072055839204 2313 98-99 1.3766977129112448 2313 100 1.53312453974862 2314 1 0.5565638965104789 2314 2 0.4865740506936973 2314 3 0.6166128347519546 2314 4 0.48931949714737755 2314 5 0.5050149586523105 2314 6 0.45866527210453256 2314 7 0.5398881689735475 2314 8 0.5309768100499994 2314 9 0.7065880400411544 2314 10-11 0.7181356706799917 2314 12-13 0.7762291794568768 2314 14-15 0.9251501405547415 2314 16-17 0.9122091303656035 2314 18-19 0.8552943476306964 2314 20-21 0.9461974849808996 2314 22-23 1.0037688004016303 2314 24-25 0.9781253117551998 2314 26-27 0.9931949547928127 2314 28-29 0.9990315085463308 2314 30-31 1.05858220238364 2314 32-33 1.0806015159675795 2314 34-35 1.0792689287189674 2314 36-37 1.0940822357757938 2314 38-39 1.0638000316416338 2314 40-41 1.1489781595586521 2314 42-43 1.243936873725218 2314 44-45 1.2177532734185093 2314 46-47 1.3179763499955612 2314 48-49 1.302045293491787 2314 50-51 1.271883684472364 2314 52-53 1.2529580745632316 2314 54-55 1.039839740210411 2314 56-57 1.0485013437031157 2314 58-59 1.04879224430789 2314 60-61 1.1067135117113835 2314 62-63 1.1088394516741644 2314 64-65 1.1285887120981428 2314 66-67 1.136011748778408 2314 68-69 1.0844708491270012 2314 70-71 1.128755559777339 2314 72-73 1.122876232966064 2314 74-75 1.0981237556217494 2314 76-77 1.1334646592424704 2314 78-79 1.1681239580532257 2314 80-81 1.090046175009153 2314 82-83 1.0824685416262199 2314 84-85 1.1784931509108212 2314 86-87 1.1935821802005684 2314 88-89 1.3073477037904837 2314 90-91 1.309780995550554 2314 92-93 1.2911411204429086 2314 94-95 1.2741550191538913 2314 96-97 1.385151779885998 2314 98-99 1.4077004019218258 2314 100 1.4778474257602383 2315 1 0.4102928704753239 2315 2 0.459063265656237 2315 3 0.4889292285992184 2315 4 0.2699191516367634 2315 5 0.32595595937659283 2315 6 0.3922208942449643 2315 7 0.41228267887072434 2315 8 0.40217801793006913 2315 9 0.5401631892585783 2315 10-11 0.4350892477012067 2315 12-13 0.48543136432950007 2315 14-15 0.7137437708179704 2315 16-17 0.7146378369378183 2315 18-19 0.5866445907146485 2315 20-21 0.6958384288929764 2315 22-23 0.5317882591174055 2315 24-25 0.6644681875240295 2315 26-27 0.7843779985622419 2315 28-29 0.8662584365809849 2315 30-31 0.8886936228514202 2315 32-33 0.8216812137126652 2315 34-35 0.8631425186018546 2315 36-37 0.8783341639851301 2315 38-39 0.8710607171904883 2315 40-41 0.976746495302983 2315 42-43 1.0069031363981011 2315 44-45 1.0039737802790256 2315 46-47 0.9747679589227687 2315 48-49 0.9690254824215216 2315 50-51 0.9640498446653538 2315 52-53 1.062444409470622 2315 54-55 0.8407371225272584 2315 56-57 0.825823414415602 2315 58-59 0.8173042142667413 2315 60-61 0.7727996531596162 2315 62-63 0.7814046091404308 2315 64-65 0.8037815765802137 2315 66-67 0.795461238884414 2315 68-69 0.8554522811006393 2315 70-71 0.7869222898923312 2315 72-73 0.5596486937836289 2315 74-75 0.8270060173345612 2315 76-77 0.8452403382825935 2315 78-79 0.8693801961356193 2315 80-81 0.6651011538244269 2315 82-83 0.6182190505757035 2315 84-85 0.8170249493011923 2315 86-87 0.9793135714274825 2315 88-89 1.0209971712552175 2315 90-91 1.1135221330514646 2315 92-93 1.112517033629885 2315 94-95 1.1522740942203775 2315 96-97 1.0456386882960338 2315 98-99 0.4449219351877538 2315 100 0.8966925240913319 2316 1 0.24258400084485388 2316 2 0.23265107015165754 2316 3 0.2649848632654468 2316 4 0.13155873571365362 2316 5 0.04406780513681241 2316 6 0.10480149289216456 2316 7 0.04051261676644202 2316 8 0.1056491072711907 2316 9 0.1464694046267283 2316 10-11 0.16235449115192324 2316 12-13 -0.031310396705698906 2316 14-15 -0.05015259369146463 2316 16-17 0.16279892808670837 2316 18-19 0.21216345232127054 2316 20-21 0.22609437180330616 2316 22-23 0.15643730382996068 2316 24-25 0.2432965927880204 2316 26-27 0.29363034268287436 2316 28-29 0.25504864353082013 2316 30-31 0.13328151718599912 2316 32-33 0.3107254528764898 2316 34-35 0.29476914760635964 2316 36-37 0.37362690827916367 2316 38-39 0.519447556927858 2316 40-41 0.5287541151900257 2316 42-43 0.4841858860522237 2316 44-45 0.45747183004074543 2316 46-47 0.27686645397908194 2316 48-49 0.19415526781426706 2316 50-51 0.27314121266822156 2316 52-53 0.35658773490217754 2316 54-55 0.014560605912286917 2316 56-57 -0.015018578360681545 2316 58-59 0.027943045841908543 2316 60-61 0.12885833861788143 2316 62-63 0.27188645792040944 2316 64-65 0.11017867579122509 2316 66-67 0.055674593100164316 2316 68-69 0.05612292722497614 2316 70-71 -0.14738263885419656 2316 72-73 -0.24715837729236512 2316 74-75 -6.737294520107184E-4 2316 76-77 0.10685006399483399 2316 78-79 0.114063615649453 2316 80-81 0.2487965149301381 2316 82-83 0.3987258193615375 2316 84-85 0.40302967401973433 2316 86-87 0.44654139969317796 2316 88-89 0.43597911601333195 2316 90-91 0.31601892724302516 2316 92-93 0.3246342310745547 2316 94-95 0.5568168773865629 2316 96-97 0.5074987859657121 2316 98-99 0.43185189913702615 2316 100 0.2785222202366455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 20.0 1 377.0 2 127.0 3 121.0 4 111.0 5 82.0 6 118.0 7 998.0 8 2954.0 9 4417.0 10 5761.0 11 8171.0 12 11516.0 13 14385.0 14 16891.0 15 20017.0 16 24318.0 17 29442.0 18 35610.0 19 41981.0 20 49166.0 21 57498.0 22 63786.0 23 64825.0 24 64880.0 25 66759.0 26 73286.0 27 83818.0 28 98140.0 29 116466.0 30 140733.0 31 174584.0 32 219482.0 33 287395.0 34 401917.0 35 550740.0 36 821597.0 37 1175988.0 38 1051610.0 39 263536.0 40 296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.247237042919515 18.557069162205558 12.500506991180755 23.695186803694174 2 16.10867469444375 19.47857830048216 41.043834128097956 23.368912876976136 3 19.591935145700923 25.56905464059755 29.03938979583022 25.799620417871306 4 12.829218833955903 15.969755976315655 37.75512044638911 33.44590474333934 5 13.33416849129927 37.10719373849968 34.23973347318092 15.318904297020126 6 30.77407224329943 36.08107530947295 18.584037688010472 14.560814759217145 7 27.708292911627613 31.952704452159793 22.26849702772162 18.070505608490976 8 26.2247220361322 35.3038570047292 20.079679085340914 18.39174187379769 9 26.770951577465695 16.134797731566493 20.85322483094503 36.24102586002278 10-11 25.700367444267417 25.84473325883316 27.71917157989614 20.73572771700328 12-13 25.733488592660276 23.596835556703173 28.014228242797117 22.655447607839438 14-15 23.112694620985017 24.955240379722433 26.037762076905956 25.894302922386597 16-17 22.360000221748074 27.489662030284002 26.976091834151966 23.174245913815955 18-19 21.896972894819868 27.001984399671315 29.037444115067213 22.0635985904416 20-21 22.99038082264483 26.407703814961884 28.72901268702726 21.872902675366024 22-23 22.932137580727616 26.21449933610674 28.510760730817143 22.3426023523485 24-25 22.440633080542767 26.07914408731308 29.005597117278974 22.474625714865184 26-27 22.401187464042913 26.338571721836928 28.510919659922184 22.749321154197972 28-29 22.084719343922536 26.882129428579987 28.659634743562734 22.373516483934743 30-31 22.910140458074892 26.447506860069154 28.68271778878566 21.959634893070294 32-33 22.869690812585617 26.52094961839262 28.285118863655264 22.3242407053665 34-35 22.920866596613017 26.584757017011885 28.49388040055861 22.000495985816492 36-37 22.690123390081613 26.518958574041374 28.314282316923773 22.476635718953244 38-39 22.72469859609353 26.746087264190365 28.33579831593448 22.193415823781628 40-41 22.7134127041854 26.14071292432164 28.681783355919578 22.464091015573377 42-43 22.5844769679363 26.436390855116077 28.16312428546543 22.81600789148219 44-45 22.461959171465264 26.85857080513881 28.286765904102445 22.392704119293484 46-47 22.49676361918129 26.51139873974615 27.868621933694186 23.12321570737837 48-49 22.75421444150969 26.558245756178746 27.899418653489093 22.78812114882247 50-51 22.624453447808328 26.750615803444035 27.853761876977668 22.771168871769977 52-53 22.50982114866473 27.042107047292802 27.514278611099062 22.933793192943405 54-55 22.373194177805637 26.855112328707264 27.756660002512508 23.01503349097459 56-57 22.79909697205584 26.730127008683937 27.439408965082272 23.031367054177952 58-59 22.39306177643213 26.93719400066889 27.81298822196641 22.856756000932567 60-61 22.705716869535053 27.089969435899253 27.575054656178484 22.629259038387207 62-63 22.786741100641763 26.897142891187976 27.412169862250146 22.903946145920116 64-65 22.428593321364644 26.793085962738182 27.53397969559277 23.244341020304407 66-67 22.53442441028841 26.64253023708456 27.454507312717514 23.368538039909517 68-69 22.19463768927941 26.755376714944752 27.67255461915455 23.377430976621294 70-71 22.303246905655644 26.557616594273014 27.696049627361123 23.443086872710214 72-73 22.489174714264514 26.854653939705482 27.51716084737123 23.139010498658774 74-75 22.735366362667744 26.942689147559573 27.29634317531634 23.02560131445634 76-77 22.61356503446209 26.767145032367008 27.182958952141973 23.43633098102893 78-79 22.447639988081598 26.807554930858945 27.330089724795254 23.414715356264207 80-81 22.39957381776092 27.01924563989438 27.351211682675945 23.229968859668755 82-83 22.859586179048804 26.744730427242082 27.730422277421884 22.665261116287226 84-85 22.99819127388313 26.997717353056206 27.080926531764156 22.923164841296508 86-87 22.39857893809728 27.284873383824642 27.376821430571948 22.939726247506137 88-89 22.66599468643575 26.66181684710483 27.38646825873909 23.28572020772033 90-91 22.93337157231988 26.662789811538666 27.332631273925053 23.071207342216397 92-93 22.934952152241042 26.782474132885813 27.326612948935004 22.955960765938144 94-95 22.45758290515261 26.79021882003749 27.463530338529978 23.28866793627992 96-97 22.60135222076613 26.724875031661515 27.28675158117251 23.387021166399848 98-99 22.430896607788945 26.887807120785844 27.09884540880631 23.5824508626189 100 22.835313150370933 26.682852757055553 26.88890268071778 23.592931411855734 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 998.0 1 766.5 2 1437.5 3 2159.5 4 1481.0 5 1089.0 6 1539.0 7 2640.0 8 4103.5 9 4945.0 10 4724.5 11 4317.5 12 4526.5 13 5124.5 14 5790.0 15 6426.5 16 7018.0 17 7660.5 18 8257.5 19 8858.5 20 9663.0 21 10711.5 22 12254.0 23 14298.0 24 16817.0 25 20105.0 26 24610.5 27 30151.0 28 36815.0 29 45526.0 30 57079.5 31 69189.5 32 83026.5 33 97411.0 34 110784.0 35 124466.5 36 138011.5 37 155846.0 38 167722.5 39 170111.5 40 170370.5 41 170068.0 42 174302.0 43 183356.0 44 194916.5 45 213042.0 46 229834.5 47 241380.5 48 252099.5 49 257076.0 50 256178.5 51 256877.5 52 252584.0 53 241990.0 54 229552.0 55 207484.5 56 185775.0 57 162169.0 58 138111.5 59 121498.0 60 99962.0 61 77445.5 62 61161.0 63 48738.0 64 36594.0 65 25281.5 66 18258.0 67 13653.0 68 10625.0 69 8221.5 70 6418.5 71 4951.5 72 3832.5 73 3125.5 74 2519.0 75 1881.5 76 1323.5 77 929.5 78 645.5 79 432.5 80 302.0 81 209.0 82 158.5 83 129.0 84 112.0 85 92.0 86 67.0 87 49.0 88 34.0 89 23.5 90 24.0 91 23.0 92 14.0 93 10.5 94 11.0 95 8.5 96 5.0 97 4.5 98 6.5 99 4.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05592397912678843 2 0.1317026253991822 3 0.015850642604574944 4 0.015552822597390865 5 0.019027389347871803 6 0.05236668459653414 7 0.05683398470429534 8 0.048577752282914444 9 0.039593515399528025 10-11 0.038427053704723706 12-13 0.039940972074576114 14-15 0.017935382654863507 16-17 0.016967467631515246 18-19 0.017099832079152617 20-21 0.02069021772131625 22-23 0.025347791722556176 24-25 0.02371805446102107 26-27 0.018622023226982358 28-29 0.01813392932631956 30-31 0.0161650081677137 32-33 0.022940413331151526 34-35 0.02328787000619962 36-37 0.02342023445383699 38-39 0.02117831162197905 40-41 0.02522370005289614 42-43 0.032454108005087426 44-45 0.041074342657471084 46-47 0.046898378353515326 48-49 0.038741419267862454 50-51 0.02663007230904319 52-53 0.03913023983279723 54-55 0.03544058085490557 56-57 0.03715304589621403 58-59 0.04211671268261537 60-61 0.02270877554778613 62-63 0.025099608383236112 64-65 0.024603241704595977 66-67 0.031047735748940382 68-69 0.03786450480226489 70-71 0.03992442651862144 72-73 0.043407266047079715 74-75 0.02602615951669769 76-77 0.019722302697967993 78-79 0.019250754353259864 80-81 0.02371805446102107 82-83 0.0200035771491974 84-85 0.011838345285567195 86-87 0.01377417533226372 88-89 0.0179105643209315 90-91 0.02019385104267612 92-93 0.01918457212944118 94-95 0.02310586889069824 96-97 0.024628060038527983 98-99 0.027308440103184704 100 0.0280281717872129 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6043919.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.34980556617775 #Duplication Level Percentage of deduplicated Percentage of total 1 79.29800368361329 53.40705129877402 2 13.247860053892403 17.844815975951725 3 3.730070102310021 7.536584884163779 4 1.4541567392532753 3.9174869460582045 5 0.709878075495703 2.390507518016402 6 0.38537291387191563 1.557287449184693 7 0.2627070193332126 1.2385286671073377 8 0.17249646786797668 0.9294082857408524 9 0.13054529476744783 0.7912980198150275 >10 0.5749423489767745 6.52409211766458 >50 0.02339304883861643 1.0689010726044759 >100 0.009471024817304543 1.1753105280099863 >500 6.677437676199448E-4 0.31136525823157485 >1k 3.483865555950348E-4 0.4640937537736429 >5k 2.9032212963732646E-5 0.18029949455885508 >10k+ 5.806442592746529E-5 0.6629687303451405 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18785 0.31080826860849725 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15121 0.2501853515905822 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9221 0.15256657145802252 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.3091111909342265E-5 7 0.0 0.0 0.0 0.0 3.3091111909342265E-5 8 0.0 0.0 0.0 0.0 3.3091111909342265E-5 9 0.0 0.0 0.0 1.6545555954671133E-5 6.618222381868453E-5 10-11 0.0 0.0 0.0 2.48183339320067E-5 6.618222381868453E-5 12-13 0.0 0.0 0.0 6.618222381868453E-5 6.618222381868453E-5 14-15 0.0 0.0 0.0 1.4063722561470464E-4 1.0754611370536236E-4 16-17 0.0 0.0 0.0 2.0681944943338917E-4 1.1581889168269793E-4 18-19 0.0 0.0 0.0 2.2336500538806028E-4 1.6545555954671133E-4 20-21 0.0 0.0 0.0 2.812744512294093E-4 1.6545555954671133E-4 22-23 0.0 0.0 0.0 4.96366678640134E-4 1.8200111550138246E-4 24-25 0.0 0.0 0.0 9.679150233482612E-4 1.985466714560536E-4 26-27 0.0 8.272777977335566E-6 0.0 0.001836556710968496 1.985466714560536E-4 28-29 0.0 1.6545555954671133E-5 0.0 0.004698937891126602 2.0681944943338917E-4 30-31 0.0 4.96366678640134E-5 0.0 0.016032643720076328 2.1509222741072473E-4 32-33 0.0 8.272777977335566E-5 0.0 0.045707098324779004 2.2336500538806028E-4 34-35 0.0 8.272777977335566E-5 0.0 0.08754253655616497 2.3163778336539586E-4 36-37 0.0 8.272777977335566E-5 0.0 0.15494913151549516 2.3163778336539586E-4 38-39 1.6545555954671133E-5 8.272777977335566E-5 0.0 0.25642302618549323 2.3163778336539586E-4 40-41 1.6545555954671133E-5 8.272777977335566E-5 0.0 0.3726390112111032 2.48183339320067E-4 42-43 1.6545555954671133E-5 8.272777977335566E-5 0.0 0.48343632666155845 2.730016732520737E-4 44-45 1.6545555954671133E-5 8.272777977335566E-5 0.0 0.6092983708087418 2.978200071840804E-4 46-47 1.6545555954671133E-5 8.272777977335566E-5 0.0 0.7615009400357615 3.143655631387515E-4 48-49 1.6545555954671133E-5 8.272777977335566E-5 0.0 0.918898813832548 3.3091111909342265E-4 50-51 1.6545555954671133E-5 9.100055775069123E-5 0.0 1.0819718133217866 3.3091111909342265E-4 52-53 1.6545555954671133E-5 9.92733357280268E-5 0.0 1.244862811695524 3.474566750480938E-4 54-55 1.6545555954671133E-5 9.92733357280268E-5 0.0 1.4309010428498463 3.640022310027649E-4 56-57 1.6545555954671133E-5 9.92733357280268E-5 0.0 1.6443800785549905 3.722750089801005E-4 58-59 1.6545555954671133E-5 9.92733357280268E-5 0.0 1.8846298237947927 3.805477869574361E-4 60-61 1.6545555954671133E-5 1.1581889168269793E-4 0.0 2.122546645644986 3.970933429121072E-4 62-63 1.6545555954671133E-5 1.2409166966003348E-4 0.0 2.369050611035654 3.970933429121072E-4 64-65 1.6545555954671133E-5 1.3236444763736906E-4 0.0 2.615595940316209 3.970933429121072E-4 66-67 1.6545555954671133E-5 1.3236444763736906E-4 0.0 2.8931559142337946 4.1363889886677834E-4 68-69 1.6545555954671133E-5 1.3236444763736906E-4 0.0 3.2038897278404956 4.1363889886677834E-4 70-71 1.6545555954671133E-5 1.3236444763736906E-4 0.0 3.5284142623354153 4.3845723279878503E-4 72-73 1.6545555954671133E-5 1.3236444763736906E-4 0.0 3.840835722649493 5.790944584134896E-4 74-75 1.6545555954671133E-5 1.3236444763736906E-4 0.0 4.151974902377083 5.790944584134896E-4 76-77 1.6545555954671133E-5 1.3236444763736906E-4 0.0 4.499530850760905 5.873672363908252E-4 78-79 1.6545555954671133E-5 1.3236444763736906E-4 0.0 4.889186966271388 6.039127923454964E-4 80-81 1.6545555954671133E-5 1.3236444763736906E-4 0.0 5.2818626457435975 6.28731126277503E-4 82-83 1.6545555954671133E-5 1.3236444763736906E-4 0.0 5.680039722570736 6.535494602095097E-4 84-85 1.6545555954671133E-5 1.3236444763736906E-4 0.0 6.083362467299777 6.618222381868453E-4 86-87 1.6545555954671133E-5 1.3236444763736906E-4 0.0 6.509848990365358 6.618222381868453E-4 88 1.6545555954671133E-5 1.3236444763736906E-4 0.0 6.8477423340716514 6.618222381868453E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9970 0.0 46.502537 1 GTATCAA 17190 0.0 40.90819 1 TACCTGG 5565 0.0 39.75186 2 ATCAACG 20205 0.0 33.85183 3 CAACGCA 20385 0.0 33.80658 5 ACCTGGG 6530 0.0 32.898872 3 TATCAAC 21220 0.0 32.76121 2 GTACCTG 6985 0.0 32.749557 1 AACGCAG 22535 0.0 31.227858 6 TCAACGC 22190 0.0 31.099022 4 GTACATG 20860 0.0 29.626884 1 GTGGTAT 4095 0.0 28.62075 1 TACATGG 21275 0.0 28.583652 2 ACGCAGA 24545 0.0 28.249485 7 GTACTAG 2970 0.0 28.05126 1 CGCAGAG 25050 0.0 27.714994 8 ACATGGG 21770 0.0 27.078032 3 CATGGGG 16915 0.0 25.706781 4 ATGGGGG 12975 0.0 25.433592 5 GCAGAGT 26875 0.0 25.13314 9 >>END_MODULE