FastQCFastQC Report
Mon 6 Jun 2016
ERR1211194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6043919
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177810.29419653043000743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149320.24705824151514938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92980.1538405792665322No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93550.048.2430841
TACCTGG57500.044.295472
GTATCAA165350.040.003461
GTACCTG70550.036.916491
ACCTGGG69400.035.4860153
ATCAACG198950.032.316743
CAACGCA200150.032.0899435
TATCAAC211050.030.9720292
AACGCAG219750.029.8266816
TCAACGC217300.029.6742534
GTACATG208550.029.3049371
GTACTAG28700.028.993391
GTGGTAT40500.028.5553651
TACATGG211800.028.155522
CCTGGGG65550.027.9625844
ACATGGG212800.027.0772323
ACGCAGA239900.026.9101497
CGCAGAG246150.026.150528
TGGGGGG123500.025.3757276
CATGGGG165750.025.292794
Produced by FastQC (version 0.11.5)