FastQCFastQC Report
Mon 6 Jun 2016
ERR1211193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13562102
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355040.2617883275026246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286330.21112508960631618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167620.12359441036500095No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216000.045.973671
GTATCAA373300.039.9777831
GTACTAG114250.035.3436621
CAACGCA422750.033.336895
ATCAACG432450.032.6324963
TATCAAC466750.031.7413982
GCATCGT117300.031.2575198
TAGGCAT139300.030.6078825
AACGCAG477150.030.2457076
GTGGTAT71800.029.3652731
TCAACGC499550.028.5127544
CTAGGCA159550.027.9604974
TAGTACT150400.027.848734
CTGTGCG150650.027.582369
GGGCACC126550.027.2963594
ACGCAGA517400.027.102567
GTACATG513350.026.5317211
CTAGTAC165400.026.488413
CGCAGAG530750.026.3838638
CTAACGC50900.025.9515933
Produced by FastQC (version 0.11.5)