FastQCFastQC Report
Mon 6 Jun 2016
ERR1211193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13562102
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327850.24173981289920984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282730.20847063382947567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164850.12155195411448756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA220250.045.492861
GTATCAA375400.038.8904571
GTACTAG109500.034.1802021
CAACGCA431000.032.2813535
ATCAACG436850.031.8690993
GGGCACC123750.031.4169494
GTGGTAT73400.030.4279881
TATCAAC479050.030.3140142
AACGCAG495350.028.8940416
TAGTACT158050.028.0733724
TCAACGC508950.027.7699684
CTGTGCG159000.027.365269
CTAACGC48700.026.927273
TCTAACG50750.026.4847452
ACGCAGA529500.026.2673537
GCATCGT132400.025.7652538
CGCAGAG542950.025.5644478
GTACATG512800.025.4168011
CTAGTAC178350.025.1942753
TAACGCC52750.024.948974
Produced by FastQC (version 0.11.5)