FastQCFastQC Report
Mon 6 Jun 2016
ERR1211192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9405661
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289260.3075381942853352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225880.24015324388153048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139100.14788965921693328No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164300.045.1745341
GTATCAA285250.039.0710951
GTACTAG67650.036.3163531
CAACGCA323850.032.7468875
ATCAACG331450.032.137843
TATCAAC353700.031.1899852
GCATCGT72500.030.7969258
AACGCAG363400.029.8038526
TCAACGC375550.028.726664
CTGTGCG91450.028.5274759
TAGGCAT92650.028.1593345
TACCTGG57400.027.9598662
GTACATG371800.027.5833741
TAGTACT94100.027.5752644
CTAACGC34850.027.2472973
CTAGGCA99600.027.1852514
TAACGCC34250.027.038084
ACGCAGA392650.026.9850447
TCTAACG35300.026.9320412
CGCAGAG399900.026.5179188
Produced by FastQC (version 0.11.5)