FastQCFastQC Report
Mon 6 Jun 2016
ERR1211192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9405661
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264110.28079897840247486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224450.23863288289892648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139690.14851694102094473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167450.046.4442181
GTATCAA286900.037.383161
GTACTAG66300.033.7578471
CAACGCA327700.031.3006745
ATCAACG334800.030.6742533
TATCAAC365300.029.1646482
AACGCAG366000.028.5900886
GTGGTAT61300.027.6136191
GGGCACC82650.027.30256394
TCAACGC382150.027.1195664
GTACATG370350.026.7252061
TAACGCC33250.026.7164154
CTAACGC33100.026.6954883
CTGTGCG92000.026.6599359
TAGTACT97350.026.4577314
TCTAACG34550.026.1160852
TAGGCAT91350.026.0266555
ACGCAGA397700.025.874087
TACATGG374000.025.6715262
CGCCTAC34500.025.3320457
Produced by FastQC (version 0.11.5)