FastQCFastQC Report
Mon 6 Jun 2016
ERR1211191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8098410
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253660.31322197814138825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202510.2500614318119236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119880.14802905755574242No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134000.048.682311
GTATCAA233600.039.5713921
CAACGCA270600.033.2478145
ATCAACG272700.033.0090143
GTACTAG48950.031.9225881
TATCAAC290050.031.6750282
TACCTGG55900.031.4055982
AACGCAG298700.030.6869476
TCAACGC300000.030.177554
GCATCGT48000.028.5944988
GTACATG301600.027.5439431
ACGCAGA327550.027.3244657
TACATGG306400.026.7896942
GTACCTG69400.026.7208021
CGCAGAG335650.026.6077298
GTGGTAT51700.026.5830631
ACATGGG309050.025.7043673
TAGGCAT65350.024.599685
ACCTGGG71650.024.2080123
CTAGGCA69050.024.0303424
Produced by FastQC (version 0.11.5)