FastQCFastQC Report
Mon 6 Jun 2016
ERR1211191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8098410
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229060.282845645009329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197620.2440232094942094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120060.1482513234079282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136650.050.26431
GTATCAA231050.038.5383451
TACCTGG53200.032.9493832
GTACTAG48800.032.0806731
CAACGCA277500.031.1718865
ATCAACG277800.031.0974673
TATCAAC296450.029.9929892
AACGCAG306250.028.7671896
TCAACGC305600.028.4685154
GTACATG302550.027.006711
GTGGTAT50550.026.5059151
TACATGG304500.026.329452
CTAACGC20400.026.0351473
ACGCAGA338850.025.555847
GTACCTG72750.025.4614011
TAGTACT71950.025.346194
ACATGGG307000.025.2154663
CGCAGAG347400.024.9131958
TAACGCC21100.024.7259084
TCTAACG22250.024.5006752
Produced by FastQC (version 0.11.5)