##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211191_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8098410 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.172218126768094 33.0 31.0 34.0 30.0 34.0 2 32.31071901768372 34.0 31.0 34.0 31.0 34.0 3 32.387962822331794 34.0 31.0 34.0 30.0 34.0 4 35.99968882780694 37.0 35.0 37.0 35.0 37.0 5 35.88419257607357 37.0 35.0 37.0 35.0 37.0 6 35.91058676950167 37.0 35.0 37.0 35.0 37.0 7 35.85133575603112 37.0 35.0 37.0 35.0 37.0 8 35.83799647091219 37.0 35.0 37.0 35.0 37.0 9 37.45100186332873 39.0 37.0 39.0 34.0 39.0 10-11 37.42809860207127 39.0 37.0 39.0 34.0 39.0 12-13 37.32725312746577 39.0 37.0 39.0 34.0 39.0 14-15 38.55664173584691 40.0 38.0 41.0 34.0 41.0 16-17 38.50527924617302 40.0 38.0 41.0 34.0 41.0 18-19 38.52692763147334 40.0 38.0 41.0 34.0 41.0 20-21 38.48663058551 40.0 38.0 41.0 34.0 41.0 22-23 38.36275546681385 40.0 38.0 41.0 34.0 41.0 24-25 38.296014402827225 40.0 38.0 41.0 34.0 41.0 26-27 38.15823890121641 40.0 38.0 41.0 33.5 41.0 28-29 38.13208407576302 40.0 37.5 41.0 33.5 41.0 30-31 38.14866757795666 40.0 37.5 41.0 33.5 41.0 32-33 38.01822049019499 40.0 37.0 41.0 33.0 41.0 34-35 37.875771602079915 40.0 37.0 41.0 33.0 41.0 36-37 37.73380762396569 40.0 37.0 41.0 33.0 41.0 38-39 37.64639595920681 40.0 37.0 41.0 33.0 41.0 40-41 37.54544293262505 40.0 36.5 41.0 32.5 41.0 42-43 37.40420730736034 40.0 36.0 41.0 32.0 41.0 44-45 37.268893708765056 40.0 36.0 41.0 31.5 41.0 46-47 37.0377094392603 39.0 36.0 41.0 31.0 41.0 48-49 36.81052391765791 39.0 35.5 41.0 31.0 41.0 50-51 36.61477246768193 39.0 35.0 41.0 30.0 41.0 52-53 36.41135296928657 39.0 35.0 40.0 30.0 41.0 54-55 36.171882196628715 38.5 35.0 40.0 29.5 41.0 56-57 35.9177328636115 38.0 35.0 40.0 29.0 41.0 58-59 35.64445298521562 38.0 34.0 40.0 28.0 41.0 60-61 35.374087876509094 37.0 34.0 40.0 28.0 41.0 62-63 35.06611538561273 37.0 34.0 40.0 28.0 41.0 64-65 34.690339893880406 36.0 34.0 39.0 27.0 41.0 66-67 34.38015795693229 36.0 33.0 39.0 26.5 40.5 68-69 34.04757921616712 35.0 33.0 39.0 26.0 40.0 70-71 33.72212279941371 35.0 33.0 38.0 26.0 40.0 72-73 33.38317324017925 35.0 32.5 37.0 26.0 39.5 74-75 33.04695921792056 35.0 32.0 37.0 25.5 39.0 76-77 32.059319113258034 34.0 30.5 36.0 24.5 38.0 78-79 32.555388403402645 35.0 32.0 36.0 25.5 38.0 80-81 32.48767103666029 35.0 32.0 35.5 25.0 37.0 82-83 32.24167083415139 35.0 32.0 35.0 25.0 37.0 84-85 31.99580250938147 34.0 32.0 35.0 25.0 36.5 86-87 31.766455390626064 34.0 31.0 35.0 24.5 36.0 88-89 31.558564026765747 34.0 31.0 35.0 24.0 36.0 90-91 31.352798080116962 34.0 31.0 35.0 24.0 35.0 92-93 31.17565176374128 34.0 31.0 35.0 23.0 35.0 94-95 30.99411822814602 34.0 31.0 35.0 23.0 35.0 96-97 30.82997934162385 34.0 30.0 35.0 21.0 35.0 98-99 30.65782567195289 34.0 30.0 35.0 20.0 35.0 100 28.864795929077435 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3300309894132951 1101 2 -0.2923322008953164 1101 3 -0.4079485382898582 1101 4 -0.23727629766361957 1101 5 -0.25637499339057257 1101 6 -0.3593709467630788 1101 7 -0.3442744004322975 1101 8 -0.3172287580552151 1101 9 -0.43430859984599124 1101 10-11 -0.4487799710906799 1101 12-13 -0.4547658563606589 1101 14-15 -0.6426918986216279 1101 16-17 -0.6440198732388964 1101 18-19 -0.7064580066911361 1101 20-21 -0.6669109338224146 1101 22-23 -0.7271963642888721 1101 24-25 -0.706103314653042 1101 26-27 -0.7719602510370365 1101 28-29 -0.835765396165634 1101 30-31 -0.6905584776404865 1101 32-33 -0.5956933480682025 1101 34-35 -0.5906441589280362 1101 36-37 -0.6000211571810539 1101 38-39 -0.6652995404815769 1101 40-41 -0.6672714718270498 1101 42-43 -0.6902847681492048 1101 44-45 -0.813226814924576 1101 46-47 -0.7418455739000933 1101 48-49 -0.7513181608949893 1101 50-51 -0.7522627578347354 1101 52-53 -0.8318620830548866 1101 54-55 -0.8219614418640049 1101 56-57 -0.8100818949683557 1101 58-59 -0.734755542625571 1101 60-61 -0.7674236871138049 1101 62-63 -0.940487185086198 1101 64-65 -0.8976233444365249 1101 66-67 -0.9524541985991135 1101 68-69 -0.979397501782266 1101 70-71 -0.9554675188469943 1101 72-73 -1.0111687033035324 1101 74-75 -0.9954053539774179 1101 76-77 -0.9180141324016979 1101 78-79 -0.8207550694034644 1101 80-81 -0.8074879122273728 1101 82-83 -0.7146952028552889 1101 84-85 -0.7221377679470287 1101 86-87 -0.9135221626957062 1101 88-89 -0.9789706629068817 1101 90-91 -1.0096859151458872 1101 92-93 -1.0067692293469648 1101 94-95 -0.91979374937684 1101 96-97 -0.9772836516015815 1101 98-99 -1.0541865406460253 1101 100 -0.83641834641098 1102 1 -0.411197424982241 1102 2 -0.38181578417850304 1102 3 -0.40088773698429847 1102 4 -0.24985493124183478 1102 5 -0.28352634637664664 1102 6 -0.37281980701019535 1102 7 -0.3867106488450105 1102 8 -0.3878467436468327 1102 9 -0.46454309929008275 1102 10-11 -0.5119336100348377 1102 12-13 -0.5378742414111599 1102 14-15 -0.6466191377346817 1102 16-17 -0.6289211552277152 1102 18-19 -0.636687177500221 1102 20-21 -0.6606641495482961 1102 22-23 -0.6417527817606512 1102 24-25 -0.7563262087339169 1102 26-27 -0.7911915844643005 1102 28-29 -0.8457050122844478 1102 30-31 -0.71941588924944 1102 32-33 -0.7327790060953632 1102 34-35 -0.7075742155458826 1102 36-37 -0.8110138415006887 1102 38-39 -0.8261397705346383 1102 40-41 -0.7935022263029481 1102 42-43 -0.7660554208894368 1102 44-45 -0.8550956558850089 1102 46-47 -0.8948344070299825 1102 48-49 -0.8715373547787308 1102 50-51 -0.857260429300247 1102 52-53 -0.9227675603561778 1102 54-55 -0.8600020053298536 1102 56-57 -0.8894002949557134 1102 58-59 -0.9660274755111047 1102 60-61 -1.0003887389055848 1102 62-63 -0.9574049955000419 1102 64-65 -0.8645845696187564 1102 66-67 -1.022846775029251 1102 68-69 -0.991472057023401 1102 70-71 -1.0047457609417236 1102 72-73 -1.056597809852967 1102 74-75 -1.1500466960209152 1102 76-77 -1.003078644438748 1102 78-79 -0.8365293916155778 1102 80-81 -0.8082513730660779 1102 82-83 -0.727940082603272 1102 84-85 -0.8716845574444356 1102 86-87 -0.8982504001158063 1102 88-89 -0.9253034954187562 1102 90-91 -0.9426681906310534 1102 92-93 -1.0065891969801157 1102 94-95 -1.0510693429556177 1102 96-97 -1.125713929660641 1102 98-99 -1.1811423055136423 1102 100 -1.2179237867541914 1103 1 -0.36806044690629847 1103 2 -0.351199231633359 1103 3 -0.38848414331096137 1103 4 -0.25556860691359873 1103 5 -0.23908825218433805 1103 6 -0.34344270399753896 1103 7 -0.4080428422703193 1103 8 -0.43836030358481537 1103 9 -0.5561810024519289 1103 10-11 -0.564961939537973 1103 12-13 -0.5589675683303312 1103 14-15 -0.7178708142622483 1103 16-17 -0.6795797222726989 1103 18-19 -0.7406839495038042 1103 20-21 -0.7281635591142859 1103 22-23 -0.7249779371254093 1103 24-25 -0.8092749102584165 1103 26-27 -0.8347885694598673 1103 28-29 -0.8429707145610408 1103 30-31 -0.7729732214812159 1103 32-33 -0.7075994749391867 1103 34-35 -0.8539839323031444 1103 36-37 -0.8768512599401888 1103 38-39 -0.8425449119730004 1103 40-41 -0.7656210608428609 1103 42-43 -0.7713950707773165 1103 44-45 -0.8097659758348854 1103 46-47 -0.9329174094152464 1103 48-49 -1.0238548975971824 1103 50-51 -1.0389782480322083 1103 52-53 -0.9930867956068781 1103 54-55 -0.9683036033798089 1103 56-57 -1.0071789384128707 1103 58-59 -1.008726573781118 1103 60-61 -1.0959408055989215 1103 62-63 -0.9838742959817139 1103 64-65 -0.9849656631809793 1103 66-67 -1.0551594287926491 1103 68-69 -1.0847557328956086 1103 70-71 -1.106260757965586 1103 72-73 -1.0662052039169083 1103 74-75 -1.0847840770735218 1103 76-77 -0.9620215976197279 1103 78-79 -0.9370527819530281 1103 80-81 -0.9469360168143197 1103 82-83 -0.9077078307010282 1103 84-85 -0.9796786766345456 1103 86-87 -0.9366331861102744 1103 88-89 -0.931305050155693 1103 90-91 -1.0026994683825379 1103 92-93 -1.0737158182770514 1103 94-95 -1.1113612221567415 1103 96-97 -1.2243265535319523 1103 98-99 -1.2444012920935812 1103 100 -1.3766846335280682 1104 1 -0.4323900604522706 1104 2 -0.37509283097081436 1104 3 -0.4232038845654422 1104 4 -0.28486621628390196 1104 5 -0.3024963634263713 1104 6 -0.38653597334471357 1104 7 -0.35542647841116093 1104 8 -0.37717538599486033 1104 9 -0.5572611634134219 1104 10-11 -0.5685765708495225 1104 12-13 -0.603279994471464 1104 14-15 -0.725383369499994 1104 16-17 -0.7049066121167442 1104 18-19 -0.7881318873973839 1104 20-21 -0.7698759927013938 1104 22-23 -0.7114595291460759 1104 24-25 -0.7570745476734544 1104 26-27 -0.8259225461559083 1104 28-29 -0.8626655918958264 1104 30-31 -0.8388199545059152 1104 32-33 -0.7822838773507996 1104 34-35 -0.7510264215107796 1104 36-37 -0.7698511586057535 1104 38-39 -0.8469745334827223 1104 40-41 -0.8322628130458938 1104 42-43 -0.6628829043580637 1104 44-45 -0.7747961037209095 1104 46-47 -0.9042210263641834 1104 48-49 -0.9282186147414677 1104 50-51 -0.7659304583679756 1104 52-53 -0.8524241226510654 1104 54-55 -0.8516722311759679 1104 56-57 -0.8533625135064966 1104 58-59 -0.9511457472422435 1104 60-61 -0.893410686958731 1104 62-63 -0.8572744878514342 1104 64-65 -0.8639549052117417 1104 66-67 -0.8648383835538525 1104 68-69 -0.9593770540056923 1104 70-71 -0.9959046007152565 1104 72-73 -0.941589060478627 1104 74-75 -0.8645554434142682 1104 76-77 -0.868044142189003 1104 78-79 -0.6021562232062969 1104 80-81 -0.6523896092186234 1104 82-83 -0.6659488966899616 1104 84-85 -0.6590530758088811 1104 86-87 -0.7127729087892405 1104 88-89 -0.81233170604294 1104 90-91 -0.7438950840286758 1104 92-93 -0.9799823298108095 1104 94-95 -0.9186502499058555 1104 96-97 -0.9015678751992517 1104 98-99 -0.9580047864796271 1104 100 -1.12754936635897 1105 1 -0.4012112708595623 1105 2 -0.3973487159858742 1105 3 -0.39799468820637784 1105 4 -0.2143232813771334 1105 5 -0.2750088144659131 1105 6 -0.3231849051796587 1105 7 -0.33548791309866743 1105 8 -0.37929276153396074 1105 9 -0.4615390862716353 1105 10-11 -0.4901289574793992 1105 12-13 -0.5299701145866891 1105 14-15 -0.7435853173079536 1105 16-17 -0.6367784418391693 1105 18-19 -0.6488926433272653 1105 20-21 -0.7872418960096184 1105 22-23 -0.72319149795333 1105 24-25 -0.7778656156964772 1105 26-27 -0.7968490126273267 1105 28-29 -0.7746838194412717 1105 30-31 -0.7158664768587997 1105 32-33 -0.7724406897701854 1105 34-35 -0.7361393765574391 1105 36-37 -0.716467153910564 1105 38-39 -0.7511755909712221 1105 40-41 -0.8006388818400225 1105 42-43 -0.7741107990199438 1105 44-45 -0.7641920431822982 1105 46-47 -0.6618342622511406 1105 48-49 -0.8737036854957339 1105 50-51 -0.9298695457055643 1105 52-53 -0.9437172019725182 1105 54-55 -0.8525425960723396 1105 56-57 -0.7849937290784226 1105 58-59 -0.8863628962594916 1105 60-61 -0.8515101384283952 1105 62-63 -0.9446300602583122 1105 64-65 -0.8975164397445141 1105 66-67 -0.8684315089746022 1105 68-69 -0.8709340893328346 1105 70-71 -0.9325486322243677 1105 72-73 -0.826865005253751 1105 74-75 -0.9434863140557894 1105 76-77 -0.914282312408428 1105 78-79 -0.8049120595713575 1105 80-81 -0.7628579533764253 1105 82-83 -0.718693295640179 1105 84-85 -0.7460375730801054 1105 86-87 -0.7295391245335381 1105 88-89 -0.771103362652525 1105 90-91 -0.7853277465499744 1105 92-93 -0.8192213166364652 1105 94-95 -0.7968858485502217 1105 96-97 -0.9795244415009066 1105 98-99 -0.9206772292627043 1105 100 -1.0171070727318465 1106 1 -0.46544343897460294 1106 2 -0.4370071579327721 1106 3 -0.4779917460099554 1106 4 -0.2503103638184925 1106 5 -0.3391186875361072 1106 6 -0.39156645967153025 1106 7 -0.40606860821658586 1106 8 -0.41242013222341 1106 9 -0.5462813163337898 1106 10-11 -0.563512646130981 1106 12-13 -0.6406703795711479 1106 14-15 -0.7684119496288346 1106 16-17 -0.7442742386696537 1106 18-19 -0.744376362921713 1106 20-21 -0.7687281072210794 1106 22-23 -0.6602413732524113 1106 24-25 -0.6588137109222529 1106 26-27 -0.7254792254091385 1106 28-29 -0.7529184055294635 1106 30-31 -0.8230708912918843 1106 32-33 -0.8029278127245547 1106 34-35 -0.7830252066174879 1106 36-37 -0.8377778970811036 1106 38-39 -0.8354845674897788 1106 40-41 -0.8424631734782153 1106 42-43 -0.8107770325397965 1106 44-45 -0.8429306004796686 1106 46-47 -0.8203171046121227 1106 48-49 -0.8764248258441185 1106 50-51 -0.9471300370861755 1106 52-53 -0.8674468966591604 1106 54-55 -0.9054403527009356 1106 56-57 -0.8551728250574442 1106 58-59 -0.8465542315265893 1106 60-61 -0.9737769264473286 1106 62-63 -0.8744044832680729 1106 64-65 -0.8757195848884507 1106 66-67 -0.8695838573818904 1106 68-69 -0.8434912235765992 1106 70-71 -0.9099225458107796 1106 72-73 -0.9658336764651665 1106 74-75 -0.9947676592763806 1106 76-77 -0.9633228299574981 1106 78-79 -0.8081273608427537 1106 80-81 -0.7163260940941711 1106 82-83 -0.781357836952381 1106 84-85 -0.7732107493563625 1106 86-87 -0.8679723035438656 1106 88-89 -0.9732607272912546 1106 90-91 -0.9674076979806081 1106 92-93 -0.8541960362495047 1106 94-95 -0.910919823092037 1106 96-97 -0.8018081284714853 1106 98-99 -0.9263402280563859 1106 100 -0.9042002493709838 1107 1 -0.41210033344320607 1107 2 -0.3834107808934597 1107 3 -0.46332299916963393 1107 4 -0.29862929883429956 1107 5 -0.2794764918995014 1107 6 -0.35202800810311174 1107 7 -0.42457881656513763 1107 8 -0.3854555736329033 1107 9 -0.5770339247463241 1107 10-11 -0.5266334864051814 1107 12-13 -0.519262970684295 1107 14-15 -0.7847739682737114 1107 16-17 -0.7457857270278438 1107 18-19 -0.7076402046006436 1107 20-21 -0.7517536491815662 1107 22-23 -0.8121200395647961 1107 24-25 -0.7527797915519798 1107 26-27 -0.7779502237821063 1107 28-29 -0.7363980900853377 1107 30-31 -0.7841342102644049 1107 32-33 -0.7574366717075023 1107 34-35 -0.7652488822533243 1107 36-37 -0.7315631886757856 1107 38-39 -0.7718998933234005 1107 40-41 -0.8579343007032847 1107 42-43 -0.7792800229790302 1107 44-45 -0.8577454246580558 1107 46-47 -0.8449219925482865 1107 48-49 -0.9190213871866106 1107 50-51 -0.9084985223541793 1107 52-53 -0.9423035265637907 1107 54-55 -0.9651164678763564 1107 56-57 -0.9495125936646716 1107 58-59 -0.9926029254895141 1107 60-61 -0.8978375595822712 1107 62-63 -0.907503767570013 1107 64-65 -0.8744012061530881 1107 66-67 -0.9622028730377181 1107 68-69 -0.8815132400118557 1107 70-71 -0.9710410714662103 1107 72-73 -0.8751906656640926 1107 74-75 -0.9218416873846778 1107 76-77 -0.9572480217419432 1107 78-79 -0.7859728288173571 1107 80-81 -0.7267421229260336 1107 82-83 -0.6770533084284907 1107 84-85 -0.6716843111194599 1107 86-87 -0.7731829261827201 1107 88-89 -0.7147344549862105 1107 90-91 -0.8676878860119928 1107 92-93 -0.9133916906383099 1107 94-95 -0.9168477702385722 1107 96-97 -0.8337522017673535 1107 98-99 -0.9254199256575895 1107 100 -0.8983880668715614 1108 1 -0.4361140883566499 1108 2 -0.399143005380477 1108 3 -0.4315602541008552 1108 4 -0.2877718278355985 1108 5 -0.31523960806193685 1108 6 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0.27323634082345194 2311 28-29 0.6566378859137174 2311 30-31 0.8492905326339937 2311 32-33 0.9784683547960427 2311 34-35 0.9904649766610518 2311 36-37 1.0338807096985434 2311 38-39 1.0676066607603047 2311 40-41 1.0535406065608655 2311 42-43 1.0750842748751737 2311 44-45 1.0671650176680245 2311 46-47 0.7059678189596426 2311 48-49 0.950903902077691 2311 50-51 1.0953955275748868 2311 52-53 0.9644794476036935 2311 54-55 0.9692403052793139 2311 56-57 1.0994210187479112 2311 58-59 1.1301849178478918 2311 60-61 1.171499087687387 2311 62-63 1.1437694051991016 2311 64-65 1.2152065705391806 2311 66-67 1.2111240829094996 2311 68-69 1.2357790054475828 2311 70-71 1.2701705753768309 2311 72-73 1.248421422815042 2311 74-75 1.218339734601777 2311 76-77 1.1768809995205523 2311 78-79 0.8931605328192589 2311 80-81 0.8927095040528243 2311 82-83 0.9756213957597168 2311 84-85 1.0411359537600937 2311 86-87 1.0822259734286206 2311 88-89 1.0677625552983443 2311 90-91 1.0882502926876754 2311 92-93 1.1906229799982384 2311 94-95 1.252498752873695 2311 96-97 1.259138227738422 2311 98-99 1.260690669636194 2311 100 1.419734134353007 2312 1 0.23282030080066107 2312 2 0.3192069773059103 2312 3 0.4438807002725085 2312 4 0.14751255851918899 2312 5 0.3215651085519795 2312 6 0.39899078756045014 2312 7 0.32090841297511474 2312 8 0.4117314417964977 2312 9 0.5618863952084254 2312 10-11 0.6402859400182805 2312 12-13 0.6987260991306385 2312 14-15 0.9118146824115385 2312 16-17 0.8811934403540462 2312 18-19 0.8247198295946347 2312 20-21 0.8133514247716178 2312 22-23 0.9425575997938509 2312 24-25 0.9478039398661409 2312 26-27 0.9885883485634182 2312 28-29 1.0375596588470373 2312 30-31 1.0112216183481308 2312 32-33 0.8605393664045309 2312 34-35 0.9769978705353566 2312 36-37 1.0633554674181553 2312 38-39 0.8899879724031692 2312 40-41 1.0107231263097631 2312 42-43 1.0647381139287617 2312 44-45 0.8267824292987243 2312 46-47 0.8732245402158583 2312 48-49 1.0439028643722637 2312 50-51 0.8858803161426252 2312 52-53 1.0389326208495504 2312 54-55 0.8940877696115024 2312 56-57 0.9469187435746491 2312 58-59 1.0924398094703989 2312 60-61 1.0809084583700752 2312 62-63 1.088471430998034 2312 64-65 1.0747956954301188 2312 66-67 1.0848042747142728 2312 68-69 1.214971098910091 2312 70-71 1.0805237115735196 2312 72-73 1.042582369137392 2312 74-75 1.1452190487119012 2312 76-77 1.117283774118082 2312 78-79 1.0438187832621537 2312 80-81 0.9730613743876759 2312 82-83 1.0067684332138924 2312 84-85 1.0424265120101843 2312 86-87 1.1280524775902165 2312 88-89 1.0991575382491519 2312 90-91 1.1125104802050458 2312 92-93 1.165388870878303 2312 94-95 1.185305740120672 2312 96-97 0.8934507346543299 2312 98-99 0.6741884020407589 2312 100 0.8672159092291771 2313 1 0.06197566639431784 2313 2 0.24674980512902778 2313 3 0.2965848093826722 2313 4 0.1937587819206712 2313 5 0.31332995935326124 2313 6 0.258469163163376 2313 7 0.28952546129247736 2313 8 0.3873153833658307 2313 9 0.5997730264998182 2313 10-11 0.38760562022761036 2313 12-13 0.4667754538236011 2313 14-15 0.5356649013001018 2313 16-17 0.784945385605802 2313 18-19 0.7465833055239841 2313 20-21 0.7145991880503928 2313 22-23 0.6956933633610944 2313 24-25 0.5706507091840152 2313 26-27 0.8035806341714888 2313 28-29 0.6128525310953776 2313 30-31 0.855235532354726 2313 32-33 0.9166932724227692 2313 34-35 0.9270325297106936 2313 36-37 0.9659197428014181 2313 38-39 0.9607457718714514 2313 40-41 0.9494824187746858 2313 42-43 1.0368036700497782 2313 44-45 0.8747606508911829 2313 46-47 0.9240883663632857 2313 48-49 1.026010447343758 2313 50-51 1.017987709272468 2313 52-53 1.0783221728377868 2313 54-55 1.0087670165580747 2313 56-57 0.7509020603352994 2313 58-59 0.7861449772831506 2313 60-61 0.6279568935018887 2313 62-63 0.9280302501274491 2313 64-65 1.0502382333843556 2313 66-67 0.9960840896307133 2313 68-69 1.1051357073142754 2313 70-71 1.0618526879764048 2313 72-73 1.0971027274838718 2313 74-75 1.1322997782968045 2313 76-77 1.0925627924725596 2313 78-79 0.944515846586782 2313 80-81 0.8894094556819141 2313 82-83 0.9505999878256262 2313 84-85 0.914144684044647 2313 86-87 0.9182755936016065 2313 88-89 0.9572394920512863 2313 90-91 0.920164192152825 2313 92-93 1.0881325698177164 2313 94-95 1.1172185694243346 2313 96-97 1.2503952243172094 2313 98-99 1.2223544190927704 2313 100 1.3041501583556325 2314 1 -0.3886442641300363 2314 2 0.008308221018893391 2314 3 0.26635387583720416 2314 4 0.16948426919395132 2314 5 0.3107985394974051 2314 6 0.38945968251521634 2314 7 0.42658228448207325 2314 8 0.4364724028181328 2314 9 0.39430857060627744 2314 10-11 0.5432901488094544 2314 12-13 0.44705258282095883 2314 14-15 0.7651224095420943 2314 16-17 0.7993414203609817 2314 18-19 0.8093031626327871 2314 20-21 0.8745232981942124 2314 22-23 0.922439561120477 2314 24-25 0.7925229616328053 2314 26-27 0.6995203331979312 2314 28-29 0.7808014127972527 2314 30-31 0.8707210890501571 2314 32-33 0.8983143343561935 2314 34-35 0.9209108928949874 2314 36-37 0.9134188977189055 2314 38-39 0.6751666616109375 2314 40-41 0.6758862447524265 2314 42-43 0.8919292654162021 2314 44-45 0.9348041252023833 2314 46-47 0.880359513679231 2314 48-49 0.9033162699128141 2314 50-51 0.9445317809971385 2314 52-53 0.9400409940123282 2314 54-55 0.8597570685400768 2314 56-57 0.8045511150031501 2314 58-59 0.9720323461121296 2314 60-61 1.034333402376248 2314 62-63 0.9265615203118571 2314 64-65 0.988101256485983 2314 66-67 0.7758600444075796 2314 68-69 0.7080538066557835 2314 70-71 0.8752954948817475 2314 72-73 1.0871972979969797 2314 74-75 1.0864814301401182 2314 76-77 0.9013467931657253 2314 78-79 0.8199216770352322 2314 80-81 0.8077524156231419 2314 82-83 0.8672503197462902 2314 84-85 0.8800034537110726 2314 86-87 0.8982248895205913 2314 88-89 0.8924807258928595 2314 90-91 0.9946740350506644 2314 92-93 0.9830377482210935 2314 94-95 1.0187943375881936 2314 96-97 0.9719485822172196 2314 98-99 1.1038235738691107 2314 100 1.1251840930809216 2315 1 0.39943615234196983 2315 2 0.44060228630638676 2315 3 0.470246605500229 2315 4 0.3239934666333326 2315 5 0.38339934805499354 2315 6 0.4690946712160269 2315 7 0.41568320692790195 2315 8 0.3347964294228021 2315 9 0.5217920593654526 2315 10-11 0.6014600347300174 2315 12-13 0.4784152534601134 2315 14-15 0.6426395069379822 2315 16-17 0.7986683746749961 2315 18-19 0.7571379275428711 2315 20-21 0.47920178862839435 2315 22-23 0.8093397278627492 2315 24-25 0.8605759265447901 2315 26-27 0.8027100366102857 2315 28-29 0.8152495841062901 2315 30-31 0.8971786659230148 2315 32-33 0.8462477960261836 2315 34-35 0.8761671623162997 2315 36-37 0.8652847082798019 2315 38-39 0.9124867308492028 2315 40-41 0.9420097140903465 2315 42-43 1.0179074130103416 2315 44-45 1.0232666347202084 2315 46-47 1.0220309145114825 2315 48-49 0.9007125852990683 2315 50-51 0.982395811929095 2315 52-53 1.019471562997495 2315 54-55 0.8538662846648819 2315 56-57 0.8573343040162911 2315 58-59 0.8068993702460929 2315 60-61 0.9288414913800125 2315 62-63 1.0449233248552687 2315 64-65 1.0900034627412722 2315 66-67 1.1357564540087282 2315 68-69 1.1011105178076406 2315 70-71 1.0120411598360235 2315 72-73 1.0761921674687898 2315 74-75 0.5000290620204382 2315 76-77 0.5027088765852348 2315 78-79 0.7754047328955735 2315 80-81 0.8954166641287102 2315 82-83 0.8899362134062159 2315 84-85 0.8935374824504301 2315 86-87 0.9099601695445187 2315 88-89 0.8953593157413167 2315 90-91 0.9224148912339345 2315 92-93 0.928338292044387 2315 94-95 1.027722215902319 2315 96-97 1.1805864638796457 2315 98-99 1.105416050508179 2315 100 1.0378883791923847 2316 1 0.3242666892041157 2316 2 0.09977073764503785 2316 3 0.25142933103963827 2316 4 0.06429698522858729 2316 5 0.2770796988826092 2316 6 0.37257230764884497 2316 7 0.44122516775455267 2316 8 0.4614981703842176 2316 9 0.5843625585391266 2316 10-11 0.6347107730735573 2316 12-13 0.42804778301633917 2316 14-15 0.5204842529112881 2316 16-17 0.8033111677488236 2316 18-19 0.7902092461574881 2316 20-21 0.7899406295996982 2316 22-23 0.8414307284062161 2316 24-25 0.4562184216882059 2316 26-27 0.7033702144265845 2316 28-29 0.9248263324101273 2316 30-31 0.522897718070368 2316 32-33 0.6590888456514463 2316 34-35 0.8005412200385891 2316 36-37 0.8754041609133623 2316 38-39 0.7615421485524152 2316 40-41 0.7779265380568674 2316 42-43 0.7558901700891028 2316 44-45 0.5504907646496306 2316 46-47 0.7157489326339075 2316 48-49 0.7961611081621882 2316 50-51 0.7819896952006999 2316 52-53 0.823150918959783 2316 54-55 0.7246903718362603 2316 56-57 0.8115695462739367 2316 58-59 0.7144875785518394 2316 60-61 0.8618083861777706 2316 62-63 0.8790308168791512 2316 64-65 0.9268308583690512 2316 66-67 0.9355457701076091 2316 68-69 0.9154061346320788 2316 70-71 0.8773608793898333 2316 72-73 0.9333853930709068 2316 74-75 0.9056600050839734 2316 76-77 0.7510533518810867 2316 78-79 0.46773132601629186 2316 80-81 0.5159411433722667 2316 82-83 0.44664129880852954 2316 84-85 0.4952626794787456 2316 86-87 0.5825870902828569 2316 88-89 0.6078633864868266 2316 90-91 0.4786796270151612 2316 92-93 0.2940722195451677 2316 94-95 0.3579357468116733 2316 96-97 0.5583708829126905 2316 98-99 0.7359258872391372 2316 100 0.37483709547213806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 0.0 11 8.0 12 31.0 13 186.0 14 712.0 15 2012.0 16 4659.0 17 9651.0 18 16485.0 19 24197.0 20 34286.0 21 47930.0 22 59523.0 23 63193.0 24 64400.0 25 69139.0 26 80493.0 27 97503.0 28 121173.0 29 149290.0 30 184184.0 31 230314.0 32 293863.0 33 387804.0 34 553806.0 35 775519.0 36 1212723.0 37 1836184.0 38 1543011.0 39 236103.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.770154773350875 19.805768320648546 13.663852991320798 23.76022391467978 2 15.692710050491392 19.76192857610321 40.741664104435316 23.80369726897008 3 19.165206503498837 25.22584062797512 29.47773698787787 26.131215880648178 4 12.73159985158242 16.26270553784327 37.83363693720266 33.17205767337164 5 13.34855854420806 37.651267347541065 34.35813943724756 14.642034671003321 6 31.34635070340968 36.62804180079793 17.704882810329433 14.320724685462949 7 27.949467737320845 32.05867466105137 21.846879691927406 18.144977909700383 8 26.51874874203702 35.78630867046741 19.363504687957267 18.331437899538304 9 26.587830753564003 15.886433506465087 20.579403948925435 36.94633179104548 10-11 25.9318784239898 26.08108121457468 27.182654197003004 20.804386164432515 12-13 25.839448938606207 23.479398666997266 27.842910606627868 22.838241787768656 14-15 23.1339452441392 24.962447840793292 25.642217708986433 26.261389206081077 16-17 22.33774657760615 27.665633724536676 26.671565583696065 23.325054114161105 18-19 21.98167911972844 27.172728967785037 28.809321829840673 22.036270082645853 20-21 23.04644510752389 26.47291421852649 28.51507447233929 21.96556620161033 22-23 23.046209070669427 26.1770335164557 28.321553243167486 22.455204169707386 24-25 22.559321670778505 26.08395752798851 28.83798137859496 22.518739422638028 26-27 22.510333715941215 26.410501521324754 28.277753935529866 22.80141082720417 28-29 22.1297361093738 26.947519094220297 28.498783647570576 22.423961148835332 30-31 23.037228231256922 26.39342769354633 28.490468124332224 22.078875950864525 32-33 22.997300371923647 26.503704694440742 28.08691963847127 22.41207529516434 34-35 22.980046220765107 26.61143691591815 28.36713926170041 22.04137760161633 36-37 22.793414225781213 26.438397788519797 28.191715966099036 22.57647201959996 38-39 22.79479117141459 26.576367476210766 28.422785571339055 22.206055781035587 40-41 22.728342535876333 25.970249329952377 28.77142192100693 22.52998621316436 42-43 22.6086688084471 26.25569672537269 28.199357836314014 22.9362766298662 44-45 22.49296908893743 26.7678976251433 28.286923907098238 22.45220937882103 46-47 22.5071587490068 26.455862393440064 27.795453586895892 23.241525270657245 48-49 22.92702286973169 26.525852250257792 27.82497708877712 22.722147791233393 50-51 22.777957772347683 26.57427480558271 27.751673934063202 22.896093488006404 52-53 22.462811790193076 26.91329445504272 27.48468738933688 23.139206365427324 54-55 22.44146346011614 26.726611966164082 27.627433930933915 23.20449064278586 56-57 22.975007581767013 26.58152696293677 27.268337503716793 23.175127951579427 58-59 22.37508175084122 26.831268239575902 27.785382060457092 23.008267949125784 60-61 22.73919534031435 27.03445406719167 27.44600957982884 22.780341012665136 62-63 22.8303615435875 26.77915172344089 27.247213470546654 23.14327326242495 64-65 22.374514732323952 26.713519259759583 27.366824070363748 23.545141937552714 66-67 22.505263653420585 26.589751213801783 27.35495399967542 23.550031133102213 68-69 22.15390532710337 26.663403171874773 27.559595743437416 23.623095757584444 70-71 22.224629983300996 26.351735635740454 27.629540205694315 23.794094175264238 72-73 22.403039030228157 26.639076554839207 27.49593483380303 23.4619495811296 74-75 22.60579184949129 26.7078264101622 27.271655974657133 23.414725765689383 76-77 22.39291358944079 26.554788954095955 27.13443279187992 23.917864664583334 78-79 22.261645675267992 26.555601333114986 27.309356430911414 23.87339656070561 80-81 22.07986740936488 26.887457190911263 27.339952863001837 23.692722536722016 82-83 22.594848803908903 26.36415692604523 27.867541836645138 23.173452433400733 84-85 22.75463413103387 26.6119973581024 27.13765269918566 23.495715811678068 86-87 21.952145252341584 27.004110531848667 27.431824779244014 23.61191943656574 88-89 22.272367632654024 26.29284315580227 27.475423181447646 23.95936603009606 90-91 22.377046831019324 26.186421979268793 27.500437335937256 23.93609385377463 92-93 22.399393618741488 26.362705625542525 27.402557825051943 23.835342930664044 94-95 21.76910112064683 26.303013986396138 27.678263181115508 24.249621711841524 96-97 21.81828509685884 26.266679015204137 27.515937331172342 24.399098556764685 98-99 21.524781175470903 26.48283800624207 27.4112752526656 24.58110556562142 100 21.671937639638376 26.341664780941294 27.222973767501422 24.763423811918905 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1225.0 1 895.5 2 1111.0 3 1583.0 4 1164.5 5 916.5 6 1408.0 7 2586.5 8 4033.5 9 4856.5 10 4575.0 11 4124.5 12 4468.0 13 5189.5 14 6100.0 15 7149.0 16 8106.5 17 9068.5 18 10091.5 19 11027.0 20 11921.5 21 13141.5 22 14850.5 23 17275.0 24 20264.0 25 24194.5 26 29807.5 27 36356.5 28 44451.5 29 56632.0 30 72753.0 31 89376.0 32 109494.0 33 130614.0 34 148599.0 35 166865.0 36 185594.5 37 211746.0 38 227502.0 39 228474.0 40 226536.5 41 222690.0 42 226607.0 43 238185.5 44 254568.0 45 284471.5 46 312272.5 47 330728.0 48 348170.0 49 353939.0 50 351266.0 51 355324.5 52 353369.0 53 337725.0 54 321178.0 55 290341.0 56 256978.5 57 221533.0 58 185833.0 59 164695.0 60 134937.5 61 101078.0 62 78136.5 63 61626.0 64 45176.0 65 29340.0 66 20192.0 67 15085.5 68 11734.0 69 9012.0 70 6947.0 71 5277.0 72 4073.0 73 3162.5 74 2413.5 75 1731.5 76 1167.5 77 698.5 78 426.5 79 289.0 80 197.0 81 131.0 82 73.5 83 41.5 84 24.5 85 12.5 86 7.5 87 6.5 88 7.0 89 8.0 90 3.5 91 1.0 92 1.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043724632366106436 2 0.0 3 0.0 4 0.028820472166758662 5 0.0 6 0.0 7 7.285380710534537E-4 8 0.0 9 0.011310862255677349 10-11 4.445317043715989E-4 12-13 2.4696205798422163E-5 14-15 9.261077174408309E-5 16-17 2.654842123330382E-4 18-19 0.0 20-21 6.174051449605541E-6 22-23 0.0 24-25 5.124462703172598E-4 26-27 5.000981674180486E-4 28-29 2.0991774928658834E-4 30-31 0.0023152692936020776 32-33 0.014613979781216315 34-35 0.0253012628404835 36-37 0.030561554675547425 38-39 0.021405436375782405 40-41 0.0012965508044171634 42-43 9.261077174408309E-5 44-45 0.01176774206294816 46-47 0.018515980297367014 48-49 0.003759997332809774 50-51 0.011650435085405654 52-53 0.011329384410026165 54-55 0.01063171659622074 56-57 4.198354985731767E-4 58-59 0.007828697238099825 60-61 0.004358880323421512 62-63 0.001475598296455724 64-65 0.03553784014392949 66-67 0.06093171375615707 68-69 0.05972777372348399 70-71 0.05204107966872509 72-73 0.045965813042313244 74-75 0.035544014195379094 76-77 0.03181488711981735 78-79 0.015854964122587027 80-81 0.0012409843413707135 82-83 0.038828609566569236 84-85 0.052207779057864445 86-87 0.05789408044295115 88-89 0.07066819289218501 90-91 0.04872561404028692 92-93 0.03918053049919676 94-95 0.013305080873899939 96-97 0.0035192093262751576 98-99 0.02148569904462728 100 0.030845561042229276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8098410.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.902518158762334 #Duplication Level Percentage of deduplicated Percentage of total 1 76.07057308510957 47.85030604833562 2 14.658291987704494 18.44086955866045 3 4.458223029270978 8.413003651635902 4 1.868114561949422 4.700364406226874 5 0.9345732561189103 2.9393505606856696 6 0.5208489684922574 1.9657627019134107 7 0.34798666779483395 1.5322466382980218 8 0.23187778466226844 1.1668557248265539 9 0.1594675312699428 0.9027818353296521 >10 0.7054266390758337 7.591328536421606 >50 0.029677333378085133 1.2723844297479354 >100 0.013622625298172361 1.5005250874563791 >500 7.949025656930176E-4 0.3449900309109477 >1k 4.4710912259821154E-4 0.5249711160935854 >5k 0.0 0.0 >10k+ 7.451818695389966E-5 0.8542596734573273 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22906 0.282845645009329 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19762 0.2440232094942094 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12006 0.1482513234079282 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 1.234810289921108E-5 0.0 6.17405144960554E-6 0.0 14-15 0.0 1.852215434881662E-5 0.0 2.469620579842216E-5 2.469620579842216E-5 16-17 0.0 2.469620579842216E-5 0.0 2.469620579842216E-5 4.939241159684432E-5 18-19 0.0 2.469620579842216E-5 0.0 2.469620579842216E-5 4.939241159684432E-5 20-21 0.0 3.704430869763324E-5 0.0 5.556646304644986E-5 6.17405144960554E-5 22-23 0.0 3.704430869763324E-5 0.0 2.2226585218579944E-4 9.261077174408309E-5 24-25 0.0 4.321836014723878E-5 0.0 5.927089391621319E-4 1.234810289921108E-4 26-27 0.0 4.939241159684432E-5 0.0 0.001191591929773869 1.6052533768974405E-4 28-29 0.0 4.939241159684432E-5 0.0 0.003475990966127919 1.6052533768974405E-4 30-31 0.0 4.939241159684432E-5 0.0 0.01389778981306207 1.6669938913934957E-4 32-33 0.0 4.939241159684432E-5 0.0 0.04002020149634311 1.852215434881662E-4 34-35 0.0 4.939241159684432E-5 0.0 0.0786882857252226 1.9756964638737728E-4 36-37 0.0 6.17405144960554E-5 0.0 0.14061402176476617 2.28439903635405E-4 38-39 0.0 8.026266884487201E-5 0.0 0.23588580968362924 2.469620579842216E-4 40-41 0.0 8.643672029447756E-5 0.0 0.3428821213052933 2.531361094338271E-4 42-43 0.0 1.1113292609289972E-4 0.0 0.44263627057656996 2.5931016088343267E-4 44-45 0.0 1.1113292609289972E-4 0.0 0.557473627539233 2.5931016088343267E-4 46-47 0.0 1.1113292609289972E-4 0.0 0.6956118546727074 2.9018041813146035E-4 48-49 0.0 1.1113292609289972E-4 0.0 0.842980288723342 2.963544695810659E-4 50-51 0.0 1.1113292609289972E-4 0.0 0.9975229705584183 2.963544695810659E-4 52-53 0.0 1.1113292609289972E-4 0.0 1.1464966579859501 3.0252852103067145E-4 54-55 0.0 1.1113292609289972E-4 0.0 1.3136837971898188 3.1487662392988254E-4 56-57 0.0 1.1113292609289972E-4 0.0 1.5072773544436502 3.210506753794881E-4 58-59 0.0 1.1113292609289972E-4 0.0 1.7295308585265503 3.210506753794881E-4 60-61 0.0 1.1113292609289972E-4 0.0 1.9553097459871753 3.210506753794881E-4 62-63 0.0 1.1113292609289972E-4 0.0 2.1864909284662053 3.2722472682909364E-4 64-65 0.0 1.1113292609289972E-4 0.0 2.4151098795936488 3.3339877827869913E-4 66-67 0.0 1.1113292609289972E-4 0.0 2.666801260988268 3.3339877827869913E-4 68-69 0.0 1.1113292609289972E-4 0.0 2.954530580694235 3.395728297283047E-4 70-71 0.0 1.1113292609289972E-4 0.0 3.2581086904713397 3.580949840771213E-4 72-73 0.0 1.1113292609289972E-4 0.0 3.553123390887841 4.198354985731767E-4 74-75 0.0 1.1113292609289972E-4 0.0 3.8440385211418047 4.198354985731767E-4 76-77 0.0 1.1113292609289972E-4 0.0 4.1681515260402975 4.198354985731767E-4 78-79 0.0 1.1113292609289972E-4 0.0 4.5250116998274965 4.198354985731767E-4 80-81 0.0 1.1113292609289972E-4 0.0 4.897733011788734 4.198354985731767E-4 82-83 0.0 1.1113292609289972E-4 0.0 5.277616223431513 4.321836014723878E-4 84-85 0.0 1.1113292609289972E-4 0.0 5.657826659801122 4.321836014723878E-4 86-87 0.0 1.1113292609289972E-4 0.0 6.054447724923781 4.321836014723878E-4 88 0.0 1.1113292609289972E-4 0.0 6.375510748406168 4.321836014723878E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13665 0.0 50.2643 1 GTATCAA 23105 0.0 38.538345 1 TACCTGG 5320 0.0 32.949383 2 GTACTAG 4880 0.0 32.080673 1 CAACGCA 27750 0.0 31.171886 5 ATCAACG 27780 0.0 31.097467 3 TATCAAC 29645 0.0 29.992989 2 AACGCAG 30625 0.0 28.767189 6 TCAACGC 30560 0.0 28.468515 4 GTACATG 30255 0.0 27.00671 1 GTGGTAT 5055 0.0 26.505915 1 TACATGG 30450 0.0 26.32945 2 CTAACGC 2040 0.0 26.035147 3 ACGCAGA 33885 0.0 25.55584 7 GTACCTG 7275 0.0 25.461401 1 TAGTACT 7195 0.0 25.34619 4 ACATGGG 30700 0.0 25.215466 3 CGCAGAG 34740 0.0 24.913195 8 TAACGCC 2110 0.0 24.725908 4 TCTAACG 2225 0.0 24.500675 2 >>END_MODULE