FastQCFastQC Report
Mon 6 Jun 2016
ERR1211190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5287713
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184540.3489977614140556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146610.27726542647076347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88370.16712329129814724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88050.047.941621
GTATCAA156550.040.5817071
TACCTGG40350.034.289212
ATCAACG183850.033.1875723
CAACGCA183450.033.1571245
TATCAAC192050.032.6150052
TCAACGC198800.030.9752834
GTGGTAT37550.030.7047621
AACGCAG201000.030.683896
GTACCTG50950.028.4482881
GTACATG171250.028.2221071
ACGCAGA218800.027.7585037
TACATGG172450.027.3437582
TGGTATC41650.027.3434962
GTACTAG24800.027.1352581
CGCAGAG224250.027.0828598
ACCTGGG51650.026.3932063
ACATGGG177000.025.6813373
GCAGAGT242500.024.1719079
TAACGCA6300.023.8764484
Produced by FastQC (version 0.11.5)