##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211190_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5287713 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.360245157027244 31.0 31.0 34.0 28.0 34.0 2 31.363566630791045 31.0 31.0 34.0 28.0 34.0 3 31.424149722195587 31.0 31.0 34.0 28.0 34.0 4 35.02107886717755 37.0 35.0 37.0 32.0 37.0 5 35.07750174792013 37.0 35.0 37.0 32.0 37.0 6 35.14109237774441 37.0 35.0 37.0 32.0 37.0 7 35.058578065791394 37.0 35.0 37.0 32.0 37.0 8 35.05969196890981 37.0 35.0 37.0 32.0 37.0 9 36.62228396283989 39.0 37.0 39.0 32.0 39.0 10-11 36.56506395108812 39.0 37.0 39.0 32.0 39.0 12-13 36.46076555213946 39.0 37.0 39.0 32.0 39.0 14-15 37.6484525540626 40.0 37.0 41.0 32.0 41.0 16-17 37.61674139651679 40.0 37.0 41.0 32.0 41.0 18-19 37.62702268447626 40.0 37.0 41.0 32.0 41.0 20-21 37.58907773549737 40.0 37.0 41.0 32.0 41.0 22-23 37.481786265631285 40.0 37.0 41.0 31.0 41.0 24-25 37.38837905915091 40.0 37.0 41.0 31.0 41.0 26-27 37.240370269717744 40.0 36.5 41.0 31.0 41.0 28-29 37.10822977722127 40.0 36.0 41.0 30.5 41.0 30-31 36.94612538539819 39.0 36.0 41.0 30.0 41.0 32-33 36.74167338507215 39.0 35.5 41.0 30.0 41.0 34-35 36.578109099340296 39.0 35.0 41.0 30.0 41.0 36-37 36.45983547140323 39.0 35.0 41.0 29.5 41.0 38-39 36.3156561258147 39.0 35.0 41.0 29.0 41.0 40-41 36.15291866256735 39.0 35.0 40.0 28.0 41.0 42-43 35.9458761282997 39.0 35.0 40.0 27.5 41.0 44-45 35.73894460610854 38.0 35.0 40.0 27.0 41.0 46-47 35.51004716027515 38.0 34.0 40.0 26.0 41.0 48-49 35.31468793786652 38.0 34.0 40.0 25.5 41.0 50-51 34.639548799263494 37.5 33.5 39.5 24.5 40.5 52-53 34.75423060971728 38.0 33.5 40.0 24.5 40.5 54-55 35.24403688324234 38.0 34.0 40.0 26.0 41.0 56-57 35.220526530089664 38.0 34.0 40.0 26.0 41.0 58-59 35.02277421259437 38.0 34.0 40.0 26.0 41.0 60-61 34.79651070320949 37.5 34.0 40.0 26.0 41.0 62-63 34.497759050084596 37.0 33.0 40.0 25.0 41.0 64-65 34.24935392673544 36.0 33.0 40.0 25.0 41.0 66-67 34.07312698325344 36.0 33.0 39.0 25.0 41.0 68-69 33.77549027339418 35.5 33.0 39.0 24.0 41.0 70-71 33.44996201571455 35.0 32.5 39.0 24.0 40.5 72-73 33.09745423021257 35.0 32.0 38.0 23.0 40.0 74-75 32.76784954100194 35.0 32.0 37.0 23.0 39.5 76-77 32.424794424356996 35.0 31.5 37.0 22.0 39.0 78-79 32.08641004154348 35.0 31.0 36.0 21.5 39.0 80-81 31.787278734681706 35.0 31.0 36.0 20.5 37.5 82-83 31.493992109632273 35.0 31.0 35.0 20.0 37.0 84-85 31.24180198509261 34.0 31.0 35.0 20.0 37.0 86-87 31.013442106256527 34.0 30.0 35.0 19.5 36.0 88-89 30.78837637367989 34.0 30.0 35.0 19.0 36.0 90-91 30.568304387927256 34.0 30.0 35.0 18.0 36.0 92-93 30.372313985271138 34.0 29.5 35.0 18.0 35.0 94-95 30.195602049506093 34.0 29.0 35.0 18.0 35.0 96-97 30.014270063447093 34.0 29.0 35.0 17.0 35.0 98-99 29.777642338001325 34.0 29.0 35.0 15.5 35.0 100 28.65242099939993 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.47810265833970433 1101 2 -0.40415703170537043 1101 3 -0.37613627238313185 1101 4 -0.32737091773005744 1101 5 -0.3050412758164853 1101 6 -0.2646834190818197 1101 7 -0.33061981346843083 1101 8 -0.34124597193582673 1101 9 -0.4541037853191128 1101 10-11 -0.468472680526844 1101 12-13 -0.5138003451968558 1101 14-15 -0.6616689431749094 1101 16-17 -0.6692149653487292 1101 18-19 -0.6131024296359797 1101 20-21 -0.6838802252570986 1101 22-23 -0.7240958258509167 1101 24-25 -0.83134266915647 1101 26-27 -0.885478651146343 1101 28-29 -0.8364074979764169 1101 30-31 -0.8981107785476468 1101 32-33 -0.9282126122609426 1101 34-35 -0.8154375792988375 1101 36-37 -0.8220654402668544 1101 38-39 -0.8789464876318718 1101 40-41 -0.8012993751215376 1101 42-43 -0.8563348440054241 1101 44-45 -0.9237790501974175 1101 46-47 -1.1285619843626833 1101 48-49 -1.0784212226298067 1101 50-51 -1.0906324020884384 1101 52-53 -1.023717349404258 1101 54-55 -0.8229490274950919 1101 56-57 -0.7718206008979749 1101 58-59 -0.707296574654201 1101 60-61 -0.688401442415568 1101 62-63 -0.8926076876759694 1101 64-65 -0.7465434560466946 1101 66-67 -0.7116968679336537 1101 68-69 -0.6636091119430745 1101 70-71 -0.7686680028581705 1101 72-73 -0.8150872898603296 1101 74-75 -0.9254325091995526 1101 76-77 -0.9248933175382845 1101 78-79 -0.981469598646914 1101 80-81 -1.1116981508514208 1101 82-83 -1.0025317673070369 1101 84-85 -1.0475397489225138 1101 86-87 -1.1402297445446905 1101 88-89 -1.153835491602706 1101 90-91 -1.15383521038666 1101 92-93 -1.3278742181016412 1101 94-95 -1.444215422707213 1101 96-97 -1.3578614077581754 1101 98-99 -1.430186171829007 1101 100 -1.5354437558275222 1102 1 -0.35921906768520273 1102 2 -0.3760358521766811 1102 3 -0.4393637711024425 1102 4 -0.24917950331151673 1102 5 -0.2819430699445178 1102 6 -0.36375473533396274 1102 7 -0.35239095553883715 1102 8 -0.37785416679725614 1102 9 -0.5552599952560087 1102 10-11 -0.5676651737477769 1102 12-13 -0.5512637201164736 1102 14-15 -0.6493140367802326 1102 16-17 -0.6978627465573268 1102 18-19 -0.6549495900089113 1102 20-21 -0.7026289828424126 1102 22-23 -0.7055457807719989 1102 24-25 -0.7087202902637841 1102 26-27 -0.7544412788202948 1102 28-29 -0.7903727293675615 1102 30-31 -0.8482868851372984 1102 32-33 -0.9007461772720973 1102 34-35 -0.8849683480092878 1102 36-37 -0.9390746646820674 1102 38-39 -0.9456864692773692 1102 40-41 -0.9188812056356497 1102 42-43 -1.0005716939536384 1102 44-45 -1.1283611937089475 1102 46-47 -1.093443431787243 1102 48-49 -0.9727688130172893 1102 50-51 -0.9777170048303105 1102 52-53 -0.9359159589237791 1102 54-55 -0.8216198714647476 1102 56-57 -0.833697578238791 1102 58-59 -0.8174771581211857 1102 60-61 -0.8157194473095046 1102 62-63 -0.9562139290356768 1102 64-65 -0.9664487878628591 1102 66-67 -0.8627248217841057 1102 68-69 -0.9344997456796662 1102 70-71 -1.010153370105364 1102 72-73 -0.9812659092906131 1102 74-75 -1.0123010856264045 1102 76-77 -1.0722856669517142 1102 78-79 -0.9993179840321069 1102 80-81 -1.1067234953725809 1102 82-83 -1.0931281539245319 1102 84-85 -0.972097572366561 1102 86-87 -1.1511715596127345 1102 88-89 -1.0991081769503026 1102 90-91 -1.242541157524851 1102 92-93 -1.2875459666033322 1102 94-95 -1.2163704026104973 1102 96-97 -1.2656392855253422 1102 98-99 -1.3357372535703647 1102 100 -1.2535074094024807 1103 1 -0.5001080630568602 1103 2 -0.5203752754525475 1103 3 -0.5492163415019142 1103 4 -0.4725382516870553 1103 5 -0.43120962382464256 1103 6 -0.4820551811753475 1103 7 -0.4743516497388782 1103 8 -0.5395191078810413 1103 9 -0.7317787173867956 1103 10-11 -0.7150731799594041 1103 12-13 -0.7431504835730891 1103 14-15 -0.9293734308046382 1103 16-17 -0.9895013802536212 1103 18-19 -0.8612002473168161 1103 20-21 -0.867318729507879 1103 22-23 -0.9327285363659286 1103 24-25 -0.9497805377464204 1103 26-27 -1.0417444002374765 1103 28-29 -1.046981543833013 1103 30-31 -1.0519904963898696 1103 32-33 -1.134925491292499 1103 34-35 -1.0779542550923153 1103 36-37 -0.9524763516008505 1103 38-39 -0.9741979605855491 1103 40-41 -1.0155734179622655 1103 42-43 -1.1146899777739705 1103 44-45 -1.0698503462269358 1103 46-47 -1.2450929431787472 1103 48-49 -1.3146524986362635 1103 50-51 -1.319881251716879 1103 52-53 -1.3112524428764658 1103 54-55 -0.9601066227983992 1103 56-57 -0.8256201265621357 1103 58-59 -0.918260987053884 1103 60-61 -0.9531326761900942 1103 62-63 -0.9637048489436566 1103 64-65 -0.9349450119659934 1103 66-67 -0.9610829049039964 1103 68-69 -0.9805310094454569 1103 70-71 -0.9256552585130109 1103 72-73 -0.9361854548030237 1103 74-75 -0.89371810041213 1103 76-77 -0.9802151563523438 1103 78-79 -1.0222233129959655 1103 80-81 -1.0705098743184926 1103 82-83 -0.9863081366858175 1103 84-85 -1.0309377037334748 1103 86-87 -1.1463736416721702 1103 88-89 -1.1803255538140682 1103 90-91 -1.2489477931449962 1103 92-93 -1.346356214450978 1103 94-95 -1.3197236725763233 1103 96-97 -1.293330899286719 1103 98-99 -1.3028897319078752 1103 100 -1.4944244797399975 1104 1 -0.54839532312862 1104 2 -0.5609875216949156 1104 3 -0.6425320572695767 1104 4 -0.5007797590328735 1104 5 -0.4098894903378678 1104 6 -0.5434376791245015 1104 7 -0.5334219451251201 1104 8 -0.49427422201032556 1104 9 -0.6959257848700062 1104 10-11 -0.6754105777211876 1104 12-13 -0.6583628303495885 1104 14-15 -0.8205503168854165 1104 16-17 -0.9095330153824506 1104 18-19 -0.9831946538937331 1104 20-21 -0.9073666835908298 1104 22-23 -0.8378260548345509 1104 24-25 -0.8291771673828308 1104 26-27 -0.9083223111661454 1104 28-29 -0.9440130010965859 1104 30-31 -0.9345532465014514 1104 32-33 -1.0405986014878934 1104 34-35 -1.0653362066364807 1104 36-37 -1.0436618244378764 1104 38-39 -1.0644164283422555 1104 40-41 -1.1477004256483525 1104 42-43 -1.0666367834366 1104 44-45 -1.1843756015376883 1104 46-47 -1.2031948336192428 1104 48-49 -1.029289336031006 1104 50-51 -1.1468144704503374 1104 52-53 -0.8964778742774797 1104 54-55 -0.8363567575240154 1104 56-57 -1.0320894125081637 1104 58-59 -0.8969901690878075 1104 60-61 -0.8918855443130695 1104 62-63 -1.0017377973841448 1104 64-65 -0.782516501629587 1104 66-67 -0.8923137837029245 1104 68-69 -1.0430918875801254 1104 70-71 -0.9268033311544954 1104 72-73 -0.95519583320403 1104 74-75 -0.9801266468376149 1104 76-77 -0.8241434276136914 1104 78-79 -0.9417022986370043 1104 80-81 -0.9497069316366726 1104 82-83 -1.0191269981249391 1104 84-85 -0.942372449601173 1104 86-87 -0.987226120376377 1104 88-89 -0.968213826619845 1104 90-91 -0.9580048401316184 1104 92-93 -1.0781684857262341 1104 94-95 -1.0526895747822742 1104 96-97 -1.1620761770282577 1104 98-99 -1.3568290401474457 1104 100 -1.1806011837467771 1105 1 -0.6299537567841149 1105 2 -0.5678730206788885 1105 3 -0.5477460169108639 1105 4 -0.46813193742509895 1105 5 -0.3984683696027531 1105 6 -0.4116455336890894 1105 7 -0.4201424441099917 1105 8 -0.5375107228418301 1105 9 -0.7705880123244953 1105 10-11 -0.6534997525998492 1105 12-13 -0.7078661314738213 1105 14-15 -0.8520786543383494 1105 16-17 -0.8108933137711745 1105 18-19 -0.7633628847955762 1105 20-21 -0.794266528461101 1105 22-23 -0.772279037392309 1105 24-25 -0.76040700993142 1105 26-27 -0.8101807628276134 1105 28-29 -0.7955749029981334 1105 30-31 -0.9341110607444421 1105 32-33 -0.861208794187462 1105 34-35 -0.8121604460626983 1105 36-37 -0.9227219119122623 1105 38-39 -0.9319325297660086 1105 40-41 -0.9557794714996888 1105 42-43 -0.9216417694306713 1105 44-45 -1.058147305289701 1105 46-47 -1.0607175101665334 1105 48-49 -1.0819079465661545 1105 50-51 -0.8600967464192166 1105 52-53 -0.8132412851114381 1105 54-55 -0.813304899973545 1105 56-57 -0.7703805130084262 1105 58-59 -0.7948936424554844 1105 60-61 -0.7281412340835942 1105 62-63 -0.7598287282463971 1105 64-65 -0.8020261217964162 1105 66-67 -0.6936672919315043 1105 68-69 -0.8263110507504408 1105 70-71 -0.6716259009686709 1105 72-73 -0.6884792575219407 1105 74-75 -0.6738572098926809 1105 76-77 -0.7485905381551774 1105 78-79 -0.7257940999959978 1105 80-81 -0.7222625765974904 1105 82-83 -0.8254638459238102 1105 84-85 -0.8208496629480067 1105 86-87 -0.9007919100684347 1105 88-89 -0.9800916101616721 1105 90-91 -0.9684278251716698 1105 92-93 -0.9845169642396137 1105 94-95 -0.9492999063187604 1105 96-97 -0.9273807161566232 1105 98-99 -0.7978428253324807 1105 100 -0.9990610326643754 1106 1 -0.5268897420194278 1106 2 -0.5142463425818384 1106 3 -0.552627370700371 1106 4 -0.4459598877450688 1106 5 -0.47956497545003174 1106 6 -0.4302632340615631 1106 7 -0.368082439856515 1106 8 -0.4399392187388145 1106 9 -0.5633453885770763 1106 10-11 -0.5569174772026315 1106 12-13 -0.5842722630588852 1106 14-15 -0.8635727096822237 1106 16-17 -0.8482033148970984 1106 18-19 -0.9146385644787927 1106 20-21 -0.715904852908892 1106 22-23 -0.7183495462748937 1106 24-25 -0.83147430895648 1106 26-27 -0.8967850891229716 1106 28-29 -0.8294984550560969 1106 30-31 -0.9273605493071457 1106 32-33 -0.9896383870326773 1106 34-35 -0.8752000724945432 1106 36-37 -0.9304002203206494 1106 38-39 -0.896605463391424 1106 40-41 -0.9792922868969995 1106 42-43 -0.895393167776291 1106 44-45 -0.9192668114127898 1106 46-47 -1.0086846333243145 1106 48-49 -0.9740334253287486 1106 50-51 -0.9115929350837746 1106 52-53 -0.8518995580829838 1106 54-55 -0.7114055861258493 1106 56-57 -0.6080078785088077 1106 58-59 -0.7321125823926238 1106 60-61 -0.7381438085539287 1106 62-63 -0.6971198323649546 1106 64-65 -0.901254965872397 1106 66-67 -0.8439680967333203 1106 68-69 -0.8126914442164193 1106 70-71 -0.7618212512513693 1106 72-73 -0.7865162074264376 1106 74-75 -0.7777313597742648 1106 76-77 -0.6517140093576543 1106 78-79 -0.667976236859058 1106 80-81 -0.6120029501524762 1106 82-83 -0.7193957995780771 1106 84-85 -0.7357168670271257 1106 86-87 -0.7582124093049813 1106 88-89 -0.6763183979535512 1106 90-91 -0.8973318843410318 1106 92-93 -0.8150511701274468 1106 94-95 -0.9585536457204675 1106 96-97 -0.8915544922461791 1106 98-99 -0.8328402804915918 1106 100 -1.0422834267374306 1107 1 -0.5809818195749124 1107 2 -0.6085850521257434 1107 3 -0.5013640599149021 1107 4 -0.44200366409521763 1107 5 -0.3559270326353854 1107 6 -0.4497480016772002 1107 7 -0.39084667681731133 1107 8 -0.3417078347355087 1107 9 -0.5373607741035258 1107 10-11 -0.5314224079957839 1107 12-13 -0.5814589696889527 1107 14-15 -0.7908088229424166 1107 16-17 -0.7569508800652613 1107 18-19 -0.7855831486932559 1107 20-21 -0.7581383204086976 1107 22-23 -0.7901583485382133 1107 24-25 -0.76056137198416 1107 26-27 -0.8305619295820179 1107 28-29 -0.8372290421474631 1107 30-31 -0.8410716373673424 1107 32-33 -0.9146549270148583 1107 34-35 -0.8290406277030584 1107 36-37 -0.9545368141477155 1107 38-39 -0.9057503065147046 1107 40-41 -0.8843920139156509 1107 42-43 -0.9787874077235159 1107 44-45 -0.9347131489608742 1107 46-47 -0.9190667116884939 1107 48-49 -0.9044831663880899 1107 50-51 -0.9214186078105442 1107 52-53 -0.813619894524372 1107 54-55 -0.7428000323850767 1107 56-57 -0.7294891604704503 1107 58-59 -0.8438221893364286 1107 60-61 -0.7983617606271451 1107 62-63 -0.7062447447301068 1107 64-65 -0.6026746344105192 1107 66-67 -0.5657276451902149 1107 68-69 -0.561884630571555 1107 70-71 -0.6646072273841312 1107 72-73 -0.6874323218582816 1107 74-75 -0.7422616784053204 1107 76-77 -0.7306409577166164 1107 78-79 -0.6884805296132406 1107 80-81 -0.6705668403308138 1107 82-83 -0.7025478776302378 1107 84-85 -0.6826345720990226 1107 86-87 -0.5901551742805893 1107 88-89 -0.6936565019335248 1107 90-91 -0.804846671626354 1107 92-93 -0.8456974806766766 1107 94-95 -0.9089046626667034 1107 96-97 -0.8970349413186334 1107 98-99 -0.7769204314304687 1107 100 -0.8870417965415065 1108 1 -0.6136313979294421 1108 2 -0.5103678005694228 1108 3 -0.5743803979300708 1108 4 -0.4265180044086634 1108 5 -0.38405818339151665 1108 6 -0.4658585380138618 1108 7 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0.7118408839343928 2116 100 0.09937835864213795 2201 1 0.866295700044379 2201 2 0.8867709631605685 2201 3 0.9261454961794122 2201 4 0.7821357281507346 2201 5 0.7606549877320674 2201 6 0.8475205804458454 2201 7 0.9053292578501271 2201 8 0.8265326988498671 2201 9 1.0332024258255785 2201 10-11 1.0140515765049614 2201 12-13 1.054182915699002 2201 14-15 1.1505419141662045 2201 16-17 1.252976982545242 2201 18-19 1.1991144272985892 2201 20-21 1.2034086307722092 2201 22-23 1.260144751966351 2201 24-25 1.2417100892817814 2201 26-27 1.262011261987496 2201 28-29 1.1889975619932969 2201 30-31 1.1848042784929902 2201 32-33 1.2322527924938882 2201 34-35 1.2329450495021064 2201 36-37 1.1969992413899533 2201 38-39 1.24683637984959 2201 40-41 1.1970180369065417 2201 42-43 1.2431430642200283 2201 44-45 1.2088956019413857 2201 46-47 1.160951735475848 2201 48-49 1.1357318024446812 2201 50-51 0.8587680994616846 2201 52-53 0.7856104052872226 2201 54-55 0.7585552907023612 2201 56-57 0.8561124255210544 2201 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0.7526659405306724 2202 86-87 0.8542454488989293 2202 88-89 0.8431946506764838 2202 90-91 0.7945912138663083 2202 92-93 0.8606826423772347 2202 94-95 0.8560905945119153 2202 96-97 0.8766443616922821 2202 98-99 0.8218699434580792 2202 100 0.8162363504739325 2203 1 0.848932820804972 2203 2 0.8269855424071118 2203 3 0.8659876232184693 2203 4 0.7334997025045169 2203 5 0.7025338177390665 2203 6 0.7125160283191718 2203 7 -0.4079946698443564 2203 8 0.4559376891187341 2203 9 0.8327338678081446 2203 10-11 0.9300333446107558 2203 12-13 0.9934291433169165 2203 14-15 1.1991048538273787 2203 16-17 1.2017571789617918 2203 18-19 1.215648888199489 2203 20-21 1.1987134364002543 2203 22-23 1.1642541011376153 2203 24-25 1.2133098097889174 2203 26-27 1.2013432940587663 2203 28-29 1.20679805298645 2203 30-31 1.2387018018756848 2203 32-33 1.2549649027903698 2203 34-35 1.2344950450174892 2203 36-37 1.2164973163804547 2203 38-39 1.195503104623377 2203 40-41 1.1874663466401856 2203 42-43 1.1260516007112997 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0.666311309019811 2204 7 0.6861883097159378 2204 8 0.7427999005307129 2204 9 0.869242730722469 2204 10-11 0.8864678815664533 2204 12-13 0.9212621732719626 2204 14-15 1.0283256701903483 2204 16-17 1.0646750297675993 2204 18-19 1.0241243784698142 2204 20-21 1.0640475994341827 2204 22-23 1.0841709113467317 2204 24-25 1.0595718886966452 2204 26-27 1.0833265165741182 2204 28-29 1.090599838420438 2204 30-31 1.1075137999360791 2204 32-33 1.1049256667069898 2204 34-35 1.151552681269223 2204 36-37 1.1043868201343585 2204 38-39 1.1364622223341527 2204 40-41 1.1695738836858354 2204 42-43 -0.06606003033481045 2204 44-45 0.9081123574545913 2204 46-47 1.1064318284194599 2204 48-49 1.1391435139874275 2204 50-51 1.159614315161562 2204 52-53 0.4716013382626656 2204 54-55 0.6344606130684269 2204 56-57 0.9324586916512843 2204 58-59 1.037610892384805 2204 60-61 1.0245332993591347 2204 62-63 1.0087332266285145 2204 64-65 1.0705203356404454 2204 66-67 1.00913167242674 2204 68-69 0.9548526634847789 2204 70-71 0.9172016977600563 2204 72-73 0.9094873705838964 2204 74-75 0.9703763266815173 2204 76-77 0.9317148739130872 2204 78-79 0.9263203964164752 2204 80-81 0.9256322981641425 2204 82-83 0.9386497473841011 2204 84-85 0.9153451880363868 2204 86-87 0.9258049745171988 2204 88-89 0.9657154474544285 2204 90-91 0.9680141623033442 2204 92-93 0.9609547810530046 2204 94-95 0.9957926345547854 2204 96-97 0.9851476297926887 2204 98-99 1.0356606957203596 2204 100 0.7024869716865858 2205 1 0.828609281993014 2205 2 0.8758472814109304 2205 3 0.9118338896837876 2205 4 0.7382908347490513 2205 5 0.6673941085112318 2205 6 0.7519328882849834 2205 7 0.7578409674938769 2205 8 0.7600811354634516 2205 9 0.9801457236288442 2205 10-11 0.9059429002646269 2205 12-13 0.9703015165652431 2205 14-15 1.1807910533614105 2205 16-17 1.196261382927517 2205 18-19 1.1687999114754035 2205 20-21 1.1764767506642002 2205 22-23 1.2405090598074224 2205 24-25 1.1835213293016622 2205 26-27 1.186735266235786 2205 28-29 1.1763268322290585 2205 30-31 1.2200047468847544 2205 32-33 1.2199565020698415 2205 34-35 1.2002919556504779 2205 36-37 1.227374115272113 2205 38-39 1.2254295084729847 2205 40-41 1.2283554843445401 2205 42-43 1.246037366124753 2205 44-45 1.2270561503513804 2205 46-47 1.259098987983613 2205 48-49 1.2736719497285591 2205 50-51 1.2357436801867436 2205 52-53 1.1032013491201766 2205 54-55 1.1453909471122614 2205 56-57 1.1089812439137106 2205 58-59 1.1345645994257367 2205 60-61 1.0993823303161747 2205 62-63 1.089517278007314 2205 64-65 1.099892680201478 2205 66-67 1.0928234404733246 2205 68-69 1.1224433961463163 2205 70-71 1.127381965353102 2205 72-73 1.1202303210166917 2205 74-75 1.0834641364670574 2205 76-77 1.0565932095697619 2205 78-79 1.086682660338166 2205 80-81 1.0168730975596212 2205 82-83 1.0988762781038055 2205 84-85 1.1430585928506325 2205 86-87 1.1555827224466668 2205 88-89 1.0832275360899395 2205 90-91 1.0840993719206864 2205 92-93 1.1149064792539818 2205 94-95 1.141891371156266 2205 96-97 1.1581552913463078 2205 98-99 1.0534635831950325 2205 100 1.175133721073042 2206 1 0.7800775280337113 2206 2 0.7437670956416476 2206 3 0.8191429239105865 2206 4 0.6703755936009301 2206 5 0.4967217865035778 2206 6 0.6207162548374683 2206 7 0.6759292566974366 2206 8 0.6562555362662224 2206 9 0.8701084200976368 2206 10-11 0.8788048891141287 2206 12-13 0.9541337263065515 2206 14-15 1.1858855865248543 2206 16-17 1.1660297324639899 2206 18-19 1.1404922748756903 2206 20-21 1.1583786060704782 2206 22-23 1.1312447547539861 2206 24-25 1.185977049124439 2206 26-27 1.1989688863021541 2206 28-29 1.1197114739226421 2206 30-31 1.1367804876234189 2206 32-33 1.2097766982210416 2206 34-35 1.2594895311450642 2206 36-37 1.2205586902758512 2206 38-39 1.2104308188370325 2206 40-41 1.1762810381910853 2206 42-43 1.1660897687718474 2206 44-45 1.1406479958075195 2206 46-47 1.1771465960429737 2206 48-49 1.233347134338402 2206 50-51 1.2632552259436949 2206 52-53 1.2069813025984288 2206 54-55 1.119033466408709 2206 56-57 1.1653995227858545 2206 58-59 1.1268966576482242 2206 60-61 1.1533105203235579 2206 62-63 1.152322255382181 2206 64-65 1.1544555130797534 2206 66-67 1.1973031862280905 2206 68-69 1.1821461525860428 2206 70-71 1.0811115468371781 2206 72-73 1.1418547605232874 2206 74-75 1.084980686401913 2206 76-77 1.1430098322597502 2206 78-79 1.07836956497572 2206 80-81 1.088821644366174 2206 82-83 1.1136028664883035 2206 84-85 1.095839855444698 2206 86-87 1.1080728623756215 2206 88-89 1.123672636034776 2206 90-91 1.0452401841126395 2206 92-93 1.0789461633980864 2206 94-95 1.0416099535256222 2206 96-97 1.094887870922907 2206 98-99 1.1626782283161674 2206 100 1.2517125140095935 2207 1 0.8592426632902423 2207 2 0.8847496798505112 2207 3 0.8718297164761459 2207 4 0.6899780968158495 2207 5 0.622454524260661 2207 6 0.6446626969527216 2207 7 0.7328655909227493 2207 8 0.7935088464304059 2207 9 0.9153350542679561 2207 10-11 0.9660169449077856 2207 12-13 0.9600215737224076 2207 14-15 1.2010039709371867 2207 16-17 1.2238844963123583 2207 18-19 1.2035931931331803 2207 20-21 1.217309082003034 2207 22-23 1.2237302568648474 2207 24-25 1.2234657332552246 2207 26-27 1.2581820819702543 2207 28-29 1.261457232057701 2207 30-31 1.2766696741474277 2207 32-33 1.3395622843287853 2207 34-35 1.3100183611451328 2207 36-37 1.3754612026362025 2207 38-39 1.3805543864907932 2207 40-41 1.4316077773033768 2207 42-43 1.4408773458284614 2207 44-45 1.4010645091943488 2207 46-47 1.4932725988123678 2207 48-49 1.5627810240916418 2207 50-51 1.504512722681028 2207 52-53 1.455581988444358 2207 54-55 1.3542879233327767 2207 56-57 1.3195058221857963 2207 58-59 1.2781298937394396 2207 60-61 1.3005118003945597 2207 62-63 1.260063531536261 2207 64-65 1.3101466866212519 2207 66-67 1.3085629251775188 2207 68-69 1.236862957810274 2207 70-71 1.2592391536933292 2207 72-73 1.2220326115878777 2207 74-75 1.2759089338932625 2207 76-77 1.2833985467697886 2207 78-79 1.2317720635374094 2207 80-81 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1.1205387959757118 2311 72-73 1.0247046308390395 2311 74-75 1.0160362256526057 2311 76-77 1.1970750496007199 2311 78-79 1.1832670195808497 2311 80-81 1.0853384245486204 2311 82-83 1.128950163605662 2311 84-85 1.1313955292710922 2311 86-87 1.1910974442069886 2311 88-89 1.2292736854829869 2311 90-91 1.2396102381728191 2311 92-93 1.272460346665163 2311 94-95 1.2957947760674067 2311 96-97 1.3049661321197341 2311 98-99 1.3988306332693483 2311 100 1.4926598009833043 2312 1 0.6287092161836227 2312 2 0.5818403817778872 2312 3 0.6519248043626042 2312 4 0.3359453106002519 2312 5 0.443761357205247 2312 6 0.49232714355814977 2312 7 0.3396406187894243 2312 8 0.5123873310408769 2312 9 0.685632142214736 2312 10-11 0.6951278447668514 2312 12-13 0.7828275184492526 2312 14-15 0.9665252030884588 2312 16-17 1.0066426284951362 2312 18-19 0.9259194752779862 2312 20-21 0.927380916503644 2312 22-23 1.0456316816353706 2312 24-25 0.9678996990167548 2312 26-27 1.0699656590557396 2312 28-29 1.0905538349060535 2312 30-31 1.118762262397297 2312 32-33 1.1386576766117926 2312 34-35 1.2115312912435456 2312 36-37 1.176595421515458 2312 38-39 1.1968425990149996 2312 40-41 1.2694088688652556 2312 42-43 1.307716647481186 2312 44-45 1.2557122849427529 2312 46-47 1.338087283650772 2312 48-49 1.401596110395218 2312 50-51 1.364186153000361 2312 52-53 1.263458008469314 2312 54-55 1.2361469448077003 2312 56-57 1.1890091122268842 2312 58-59 1.1316251059694693 2312 60-61 1.1707556575067883 2312 62-63 1.1182295128098403 2312 64-65 1.1568619351118627 2312 66-67 1.1139487229376854 2312 68-69 1.1052446665083622 2312 70-71 1.0345659119264639 2312 72-73 1.044363053056486 2312 74-75 1.0178327317835283 2312 76-77 1.0508000645400202 2312 78-79 1.0659124980527146 2312 80-81 1.11481736788976 2312 82-83 1.0955655232599035 2312 84-85 1.1294272605579785 2312 86-87 1.0576151098635052 2312 88-89 1.012710182089485 2312 90-91 1.0221036522214177 2312 92-93 1.1171807320858562 2312 94-95 1.153855281261361 2312 96-97 1.1977979233546279 2312 98-99 0.9020384883763057 2312 100 1.1785853310607877 2313 1 0.6443444191913557 2313 2 0.633205184180035 2313 3 0.6252989802826718 2313 4 0.5161367261633529 2313 5 0.4199485702937906 2313 6 0.5066293622699618 2313 7 0.6102154425325423 2313 8 0.6615706195623758 2313 9 0.7695337633552626 2313 10-11 0.8208117437030964 2313 12-13 0.8954865563843271 2313 14-15 1.032730031345828 2313 16-17 1.0666916900914245 2313 18-19 1.076715458903287 2313 20-21 1.050176966650426 2313 22-23 1.0886310821405658 2313 24-25 1.0231946286171905 2313 26-27 1.0613820586847424 2313 28-29 1.1089567673412617 2313 30-31 1.2021833309526926 2313 32-33 1.2364524945082351 2313 34-35 1.2262663253628574 2313 36-37 1.2649103271962119 2313 38-39 1.3718768465578606 2313 40-41 1.364474427588597 2313 42-43 1.4216465061891554 2313 44-45 1.3386998117642364 2313 46-47 1.4264101288306463 2313 48-49 1.4498998977717221 2313 50-51 1.4749771294816085 2313 52-53 1.3745103622778387 2313 54-55 1.279746190750643 2313 56-57 1.1660266416390996 2313 58-59 1.212232436075709 2313 60-61 1.2071701827127157 2313 62-63 1.1741368608087512 2313 64-65 1.2482234044271223 2313 66-67 1.222995814736727 2313 68-69 1.2062276315992122 2313 70-71 1.1884256656086407 2313 72-73 1.2189898565249777 2313 74-75 1.245314509005091 2313 76-77 0.9911124630421639 2313 78-79 1.0963692932521063 2313 80-81 1.1145640051988117 2313 82-83 1.1678980919969355 2313 84-85 1.232539188560903 2313 86-87 1.2959662366913136 2313 88-89 1.3245309291377438 2313 90-91 1.3241847627454568 2313 92-93 1.370012303100843 2313 94-95 1.4114012116394186 2313 96-97 1.4321236489947928 2313 98-99 1.4468010086909047 2313 100 1.5819989450025673 2314 1 0.5962141196896091 2314 2 0.5642226252052325 2314 3 0.6376324679958394 2314 4 0.47469871940667474 2314 5 0.5021032355111359 2314 6 0.5365810467390091 2314 7 0.5246493238899319 2314 8 0.5501109850188897 2314 9 0.7490178035485613 2314 10-11 0.7286451931865585 2314 12-13 0.6908277918223433 2314 14-15 0.9234552975779877 2314 16-17 0.8960387364448223 2314 18-19 0.8630377465299617 2314 20-21 0.898749245389304 2314 22-23 0.9429692406959589 2314 24-25 0.9037381186825826 2314 26-27 0.973227143635043 2314 28-29 0.9995379294951263 2314 30-31 1.022904818044445 2314 32-33 1.0402525882075437 2314 34-35 1.011387993229981 2314 36-37 1.0047325201008732 2314 38-39 1.017597992747774 2314 40-41 1.1484947998826271 2314 42-43 1.198578409098367 2314 44-45 1.2455421621947735 2314 46-47 1.2197440855273456 2314 48-49 1.1482085498117485 2314 50-51 1.1497093145601411 2314 52-53 1.1176480082142675 2314 54-55 0.9803889002969228 2314 56-57 0.9579809198841573 2314 58-59 0.978263941736877 2314 60-61 0.9794994115878808 2314 62-63 0.9738053581183053 2314 64-65 0.998503540515145 2314 66-67 1.0772606428710674 2314 68-69 1.011702734320238 2314 70-71 1.0194219341944901 2314 72-73 1.0341360766297143 2314 74-75 0.985855310815964 2314 76-77 1.032863149837624 2314 78-79 0.951170420857995 2314 80-81 0.959050161418542 2314 82-83 1.0379571094106943 2314 84-85 1.0189419168470373 2314 86-87 1.0602571097263223 2314 88-89 1.1554418626876952 2314 90-91 1.1914042504479099 2314 92-93 1.2519402442436487 2314 94-95 1.2716331369020466 2314 96-97 1.2961359808753947 2314 98-99 1.3447651794203885 2314 100 1.4036577538817951 2315 1 0.4380624991223243 2315 2 0.47576054498469844 2315 3 0.5216673324668406 2315 4 0.10225550207148615 2315 5 0.28232619324170116 2315 6 0.3380057695296017 2315 7 0.4199482881925576 2315 8 0.4165554074402138 2315 9 0.5564779393619119 2315 10-11 0.4180362959459458 2315 12-13 0.4558350614528024 2315 14-15 0.6915928036160182 2315 16-17 0.6971916309671471 2315 18-19 0.6289340851270069 2315 20-21 0.7747892560723884 2315 22-23 0.5724678989518281 2315 24-25 0.6815861784867252 2315 26-27 0.7986423068435897 2315 28-29 0.8353841185602207 2315 30-31 0.8684687237233035 2315 32-33 0.8187633613943532 2315 34-35 0.8578953311294626 2315 36-37 0.9112532190508347 2315 38-39 0.9675527360286722 2315 40-41 0.9755211718798975 2315 42-43 1.0613703191358823 2315 44-45 1.078027128013467 2315 46-47 1.0688146790502557 2315 48-49 1.0509613629391765 2315 50-51 0.9412919727420928 2315 52-53 1.0761183095121893 2315 54-55 0.9418657978936409 2315 56-57 0.8913594899398021 2315 58-59 0.9271593394452893 2315 60-61 0.9115590338358572 2315 62-63 0.8245763900746823 2315 64-65 0.887757733300603 2315 66-67 0.8386985227449486 2315 68-69 0.8192518838130738 2315 70-71 0.7806307709168294 2315 72-73 0.5677223932630966 2315 74-75 0.7985081032347239 2315 76-77 0.8695298508987577 2315 78-79 0.8003406823463344 2315 80-81 0.5868278765397399 2315 82-83 0.622412230270843 2315 84-85 0.7975035858457282 2315 86-87 0.8503271712548255 2315 88-89 0.9354181641003194 2315 90-91 1.0255077678740285 2315 92-93 1.0422671844455138 2315 94-95 1.0042330682250942 2315 96-97 0.8746514573055499 2315 98-99 0.30403872749197447 2315 100 0.8232875318431141 2316 1 0.2835178006704737 2316 2 0.16093481041072977 2316 3 0.23823668168623868 2316 4 0.14633807740234062 2316 5 0.15596354633707676 2316 6 0.19052458527163196 2316 7 0.15378659170017528 2316 8 0.14673989505394047 2316 9 0.20108165844614234 2316 10-11 0.20723604630258308 2316 12-13 -0.0074712256065936344 2316 14-15 0.06079722248509967 2316 16-17 0.2159026768246406 2316 18-19 0.296828278506176 2316 20-21 0.3210434371775577 2316 22-23 0.276251328987577 2316 24-25 0.358170059326298 2316 26-27 0.422297803174871 2316 28-29 0.3558182514228392 2316 30-31 0.23702452782251981 2316 32-33 0.44478587584909945 2316 34-35 0.3864701470690548 2316 36-37 0.3520197643809553 2316 38-39 0.5076042959353728 2316 40-41 0.5798221643314605 2316 42-43 0.4992751533928086 2316 44-45 0.5373151259341213 2316 46-47 0.3480446621408575 2316 48-49 0.28826209130848923 2316 50-51 0.292174845782867 2316 52-53 0.41689937416200706 2316 54-55 0.2206043050880595 2316 56-57 0.018036438246575415 2316 58-59 0.05521597794412969 2316 60-61 0.20283383105030595 2316 62-63 0.23664568219137294 2316 64-65 0.26454046589807234 2316 66-67 0.1685549939002371 2316 68-69 0.1243190175706772 2316 70-71 -0.061341809946412695 2316 72-73 -0.20197276347222726 2316 74-75 0.023022675872340415 2316 76-77 0.20987214255929842 2316 78-79 0.2608934081037724 2316 80-81 0.41827823215188786 2316 82-83 0.4318126169039118 2316 84-85 0.46491867479205595 2316 86-87 0.5195911907721182 2316 88-89 0.48692439804048604 2316 90-91 0.3351619232616905 2316 92-93 0.39118933430400205 2316 94-95 0.537883756007492 2316 96-97 0.48960084974132556 2316 98-99 0.3053045671046348 2316 100 0.25527490358321003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 23.0 1 286.0 2 143.0 3 124.0 4 76.0 5 87.0 6 108.0 7 607.0 8 1798.0 9 3047.0 10 4304.0 11 6400.0 12 9359.0 13 11757.0 14 14487.0 15 16675.0 16 20274.0 17 25194.0 18 30663.0 19 36317.0 20 42715.0 21 50452.0 22 55867.0 23 57887.0 24 57708.0 25 59253.0 26 64040.0 27 73079.0 28 85313.0 29 101721.0 30 122028.0 31 150011.0 32 188401.0 33 246131.0 34 344492.0 35 477201.0 36 710567.0 37 1030018.0 38 941043.0 39 247734.0 40 323.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.27206587499404 18.63319529901171 12.54311522598547 23.55162360000878 2 16.178444547127935 19.598827388211767 40.8010953466704 23.421632717989898 3 19.582999167738517 25.410248165241732 29.146345615495196 25.860407051524554 4 12.909371331683698 16.06788269559089 37.75552666193288 33.26721931079252 5 13.407459614342077 36.94488044801116 34.49304607413309 15.154613863513672 6 30.856383614810913 36.157418550121776 18.43248625157238 14.553711583494925 7 27.562301159215867 32.05007367504603 22.28388668934979 18.10373847638832 8 25.97863704797478 35.26270209763959 20.204134959001017 18.554525895384618 9 26.51869375479845 16.14912472883678 20.914445805384556 36.41773571098022 10-11 25.49478920075348 25.85380172333957 27.838747830311718 20.812661245595233 12-13 25.704582190558185 23.559351262869612 28.087526746212298 22.648539800359906 14-15 23.02992226011792 24.99110509269146 26.093080547097802 25.885892100092818 16-17 22.309282047731834 27.585389308362394 26.984901601842783 23.120427042062985 18-19 21.88536129882702 26.981571653633264 29.14460937720183 21.988457670337887 20-21 22.91972608888634 26.381909904190888 28.896529162238416 21.80183484468436 22-23 22.87041445650124 26.15468197547315 28.6516321505366 22.323271417489018 24-25 22.43072065350793 26.049499623942385 29.099368495603077 22.42041122694661 26-27 22.372411197133946 26.284409396392082 28.712006143707526 22.631173262766445 28-29 22.0624741571495 26.775510227243064 28.786202216025615 22.375813399581823 30-31 22.84035862564665 26.374230401846088 28.834636882075337 21.95077409043192 32-33 22.77760238372809 26.432755529215974 28.50223086728879 22.287411219767147 34-35 22.81628658296559 26.572043451720806 28.63755509068268 21.974114874630924 36-37 22.655138090946856 26.428861654821432 28.45540427759984 22.46059597663187 38-39 22.600328432632203 26.686309950706733 28.494022981961365 22.219338634699703 40-41 22.72774864603691 26.109788173502114 28.74938840999462 22.413074770466356 42-43 22.511357286010647 26.433437620719047 28.29768972352429 22.757515369746013 44-45 22.3908147620922 26.890803363034266 28.387182766819073 22.331199108054463 46-47 22.421064710991516 26.511279280942944 28.02427798610062 23.043378021964923 48-49 22.713447353800813 26.5323721379987 27.997965782886485 22.756214725314 50-51 22.651459783420997 26.723564631350428 27.90860206764857 22.716373517580006 52-53 22.491995486236547 27.026485015026168 27.63365222737237 22.847867271364912 54-55 22.325120174173446 26.87672201878801 27.841341050790092 22.956816756248458 56-57 22.740583634770474 26.717690335808182 27.505246988001385 23.036479041419966 58-59 22.344531781386667 26.93259078537974 27.875120260415237 22.847757172818355 60-61 22.65900842418653 27.032947763498544 27.693072047891004 22.61497176442392 62-63 22.684263263797813 26.841317934466474 27.58408758535878 22.89033121637693 64-65 22.388230373599207 26.722646298880505 27.659779732357848 23.229343595162444 66-67 22.49426341208532 26.637366770175436 27.571925258400665 23.296444559338582 68-69 22.209009332508586 26.72020267654193 27.75963698786349 23.31115100308599 70-71 22.33121508001367 26.545532355998613 27.792872872353964 23.330379691633752 72-73 22.471441254669134 26.846294328077796 27.625945355842607 23.056319061410466 74-75 22.7199820366463 26.927598755882638 27.353178526149957 22.9992406813211 76-77 22.574558296534562 26.790576614424864 27.286147048169585 23.34871804087099 78-79 22.410329448311664 26.81690171216426 27.41661743121357 23.356151408310502 80-81 22.372084594499555 27.032742053588777 27.409980586189995 23.185192765721677 82-83 22.7774959369524 26.76536391892669 27.78652619265958 22.670613951461334 84-85 22.893690689920493 26.984757456151304 27.19989657736913 22.921655276559076 86-87 22.37834866730446 27.26766678885422 27.399841194003198 22.95414334983811 88-89 22.641811542398454 26.639083208784385 27.427328373317145 23.291776875500013 90-91 22.873664069325155 26.635554811119043 27.407786841639094 23.082994277916708 92-93 22.879582602682163 26.785882329797488 27.386780949163516 22.947754118356837 94-95 22.4176027472277 26.79236499509221 27.539268471555715 23.250763786124374 96-97 22.56215592282343 26.719532281090636 27.34506867876445 23.373243117321486 98-99 22.396303251774526 26.866924536073896 27.20098822089499 23.53578399125659 100 22.721579510403984 26.73066354911236 26.94242266885728 23.605334271626376 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1379.0 1 1037.5 2 1925.5 3 2775.5 4 1747.5 5 1239.5 6 1788.0 7 3035.0 8 4883.0 9 5699.5 10 4902.5 11 4176.5 12 4231.5 13 4743.5 14 5300.0 15 5803.0 16 6414.5 17 7088.0 18 7647.0 19 8187.0 20 8911.5 21 9784.0 22 11128.0 23 13029.5 24 15292.5 25 18380.5 26 22460.5 27 27185.5 28 33164.0 29 41323.0 30 51849.0 31 62036.0 32 73932.5 33 86560.0 34 97557.5 35 108751.0 36 119935.5 37 135184.0 38 144964.0 39 146558.5 40 147438.5 41 147696.5 42 151229.0 43 160076.0 44 170875.5 45 185017.5 46 198621.5 47 209751.5 48 219722.5 49 223230.0 50 220750.5 51 219878.0 52 216875.0 53 208652.0 54 198485.5 55 180308.0 56 161231.0 57 141311.0 58 121634.5 59 106469.0 60 87682.0 61 69308.5 62 55134.0 63 43690.0 64 33099.5 65 23230.0 66 16817.5 67 12873.0 68 10114.0 69 7899.0 70 6285.0 71 4853.0 72 3819.0 73 3120.0 74 2537.5 75 1931.0 76 1387.5 77 972.0 78 651.0 79 443.0 80 321.5 81 254.5 82 167.5 83 112.5 84 91.5 85 76.0 86 59.0 87 45.5 88 36.5 89 32.0 90 29.5 91 20.5 92 15.0 93 14.5 94 10.0 95 7.5 96 8.5 97 5.5 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057435038550692895 2 0.13603234517455845 3 0.017266443923866517 4 0.016774737963274482 5 0.020027562010267955 6 0.051912802377890026 7 0.05711353849953656 8 0.04883018424033226 9 0.0406792123551335 10-11 0.03978090338866728 12-13 0.04068866823899103 14-15 0.018533532360776764 16-17 0.017531208671877616 18-19 0.01724753215615144 20-21 0.020944782744449254 22-23 0.02541741580906528 24-25 0.024377268584735972 26-27 0.01896850301822357 28-29 0.017985091097039496 30-31 0.016320855538112602 32-33 0.02276976832895431 34-35 0.022731944793524157 36-37 0.022637385954948765 38-39 0.020585459157862765 40-41 0.024348900933163355 42-43 0.03175285799361652 44-45 0.041388403644448936 46-47 0.047534728151849386 48-49 0.04049955056184025 50-51 0.027734107354162377 52-53 0.03974307985323712 54-55 0.036830667625115054 56-57 0.038986609144633984 58-59 0.0436483598864008 60-61 0.022854871283672168 62-63 0.025530886415355752 64-65 0.025077003990193872 66-67 0.031913608019194684 68-69 0.03875966793205305 70-71 0.040613021168130724 72-73 0.044168433498565446 74-75 0.026381915962534277 76-77 0.02038688559685444 78-79 0.019696606075254086 80-81 0.024017944998149483 82-83 0.019687150191396545 84-85 0.012651972601387406 86-87 0.014354031695744455 88-89 0.018334958799768444 90-91 0.020604370925577844 92-93 0.019810076681544556 94-95 0.023072356612395567 96-97 0.02439618035245105 98-99 0.027336960232145734 100 0.02870806339148891 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5287713.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.96240155640815 #Duplication Level Percentage of deduplicated Percentage of total 1 80.38762381352899 55.43723593594064 2 12.683962393588292 17.49433015826031 3 3.4515324465040074 7.140778996823436 4 1.3555393970186185 3.739250088909174 5 0.6679256252237409 2.30308775882473 6 0.3747629909988513 1.5506733524246015 7 0.2459441913138682 1.1872631457297134 8 0.17673782631756768 0.9750611958975058 9 0.11236006182077635 0.6973757731968501 >10 0.5156149783370202 5.909081630289494 >50 0.018783549018339393 0.8717313003187734 >100 0.008130957307549631 0.9949538506457497 >500 6.681519274180479E-4 0.3075991849275419 >1k 3.1816699613847194E-4 0.47108529641347724 >5k 3.181669961377495E-5 0.19389756704243621 >10k+ 6.36333992275499E-5 0.7265947643555817 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18454 0.3489977614140556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14661 0.27726542647076347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8837 0.16712329129814724 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8911767715078333E-5 2 0.0 0.0 0.0 0.0 1.8911767715078333E-5 3 0.0 0.0 0.0 0.0 1.8911767715078333E-5 4 0.0 0.0 0.0 0.0 1.8911767715078333E-5 5 0.0 1.8911767715078333E-5 0.0 0.0 1.8911767715078333E-5 6 3.782353543015667E-5 1.8911767715078333E-5 0.0 0.0 1.8911767715078333E-5 7 3.782353543015667E-5 1.8911767715078333E-5 0.0 0.0 1.8911767715078333E-5 8 3.782353543015667E-5 1.8911767715078333E-5 0.0 0.0 1.8911767715078333E-5 9 3.782353543015667E-5 1.8911767715078333E-5 0.0 0.0 1.8911767715078333E-5 10-11 3.782353543015667E-5 1.8911767715078333E-5 0.0 0.0 1.8911767715078333E-5 12-13 3.782353543015667E-5 2.83676515726175E-5 0.0 0.0 1.8911767715078333E-5 14-15 7.564707086031333E-5 3.782353543015667E-5 0.0 0.0 1.8911767715078333E-5 16-17 7.564707086031333E-5 3.782353543015667E-5 0.0 3.782353543015667E-5 1.8911767715078333E-5 18-19 7.564707086031333E-5 3.782353543015667E-5 0.0 6.619118700277416E-5 1.8911767715078333E-5 20-21 7.564707086031333E-5 3.782353543015667E-5 0.0 9.455883857539167E-5 1.8911767715078333E-5 22-23 7.564707086031333E-5 3.782353543015667E-5 0.0 3.215000511563316E-4 3.782353543015667E-5 24-25 7.564707086031333E-5 3.782353543015667E-5 0.0 7.659265924606725E-4 6.619118700277416E-5 26-27 7.564707086031333E-5 3.782353543015667E-5 0.0 0.001645323791211815 7.564707086031333E-5 28-29 7.564707086031333E-5 3.782353543015667E-5 0.0 0.004737397812627123 7.564707086031333E-5 30-31 7.564707086031333E-5 3.782353543015667E-5 0.0 0.01565894366808486 7.564707086031333E-5 32-33 7.564707086031333E-5 3.782353543015667E-5 0.0 0.04205977139833421 7.564707086031333E-5 34-35 7.564707086031333E-5 3.782353543015667E-5 0.0 0.08437485166082198 7.564707086031333E-5 36-37 7.564707086031333E-5 5.6735303145235E-5 0.0 0.14846683244722247 7.564707086031333E-5 38-39 7.564707086031333E-5 7.564707086031333E-5 0.0 0.24677965691405718 7.564707086031333E-5 40-41 7.564707086031333E-5 7.564707086031333E-5 0.0 0.3607703368166918 7.564707086031333E-5 42-43 7.564707086031333E-5 9.455883857539167E-5 0.0 0.4668842654659964 8.51029547178525E-5 44-45 7.564707086031333E-5 1.0401472243293084E-4 0.0 0.5925435060488343 9.455883857539167E-5 46-47 7.564707086031333E-5 1.1347060629047E-4 0.0 0.7365282495475832 1.1347060629047E-4 48-49 7.564707086031333E-5 1.1347060629047E-4 0.0 0.8893542444531313 1.3238237400554833E-4 50-51 7.564707086031333E-5 1.3238237400554833E-4 0.0 1.0488466374782444 1.3238237400554833E-4 52-53 7.564707086031333E-5 1.5129414172062667E-4 0.0 1.205540088881526 1.5129414172062667E-4 54-55 7.564707086031333E-5 2.080294448658617E-4 0.0 1.3852775292456303 1.70205909435705E-4 56-57 7.564707086031333E-5 2.2694121258094E-4 0.0 1.5877374585194015 1.70205909435705E-4 58-59 7.564707086031333E-5 2.6476474801109665E-4 0.0 1.8183570099209243 1.70205909435705E-4 60-61 9.455883857539167E-5 3.0258828344125333E-4 0.0 2.0484943112457126 1.70205909435705E-4 62-63 9.455883857539167E-5 3.120441672987925E-4 0.0 2.279302980324386 1.70205909435705E-4 64-65 9.455883857539167E-5 3.215000511563317E-4 0.0 2.512872767489461 1.7966179329324418E-4 66-67 9.455883857539167E-5 3.4041181887141E-4 0.0 2.780578295380252 1.8911767715078335E-4 68-69 9.455883857539167E-5 3.4041181887141E-4 0.0 3.0860317116303397 1.8911767715078335E-4 70-71 9.455883857539167E-5 3.4986770272894916E-4 0.0 3.4079572775602607 2.0802944486586166E-4 72-73 9.455883857539167E-5 3.5932358658648836E-4 0.0 3.7103469874405057 2.2694121258094E-4 74-75 1.1347060629047E-4 3.5932358658648836E-4 0.0 4.009077270267883 2.2694121258094E-4 76-77 1.1347060629047E-4 3.5932358658648836E-4 0.0 4.348373294844103 2.2694121258094E-4 78-79 1.1347060629047E-4 3.5932358658648836E-4 0.0 4.727535325763709 2.2694121258094E-4 80-81 1.1347060629047E-4 3.8769123815910584E-4 0.0 5.1112740044703635 2.2694121258094E-4 82-83 1.1347060629047E-4 3.97147122016645E-4 0.0 5.497367198257546 3.4041181887141E-4 84-85 1.1347060629047E-4 3.97147122016645E-4 0.0 5.883536039115587 3.4041181887141E-4 86-87 1.1347060629047E-4 3.97147122016645E-4 0.0 6.290025574383481 3.4041181887141E-4 88 1.1347060629047E-4 3.97147122016645E-4 0.0 6.623752083367611 3.4041181887141E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8805 0.0 47.94162 1 GTATCAA 15655 0.0 40.581707 1 TACCTGG 4035 0.0 34.28921 2 ATCAACG 18385 0.0 33.187572 3 CAACGCA 18345 0.0 33.157124 5 TATCAAC 19205 0.0 32.615005 2 TCAACGC 19880 0.0 30.975283 4 GTGGTAT 3755 0.0 30.704762 1 AACGCAG 20100 0.0 30.68389 6 GTACCTG 5095 0.0 28.448288 1 GTACATG 17125 0.0 28.222107 1 ACGCAGA 21880 0.0 27.758503 7 TACATGG 17245 0.0 27.343758 2 TGGTATC 4165 0.0 27.343496 2 GTACTAG 2480 0.0 27.135258 1 CGCAGAG 22425 0.0 27.082859 8 ACCTGGG 5165 0.0 26.393206 3 ACATGGG 17700 0.0 25.681337 3 GCAGAGT 24250 0.0 24.171907 9 TAACGCA 630 0.0 23.876448 4 >>END_MODULE