FastQCFastQC Report
Mon 6 Jun 2016
ERR1211190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5287713
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167420.31662081508584144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139790.26436760088908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89960.1701302623648447No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88050.047.524771
GTATCAA148700.039.1759641
TACCTGG45000.037.6995662
ATCAACG180900.031.2318633
CAACGCA181350.031.1464085
GTACCTG55750.030.951421
ACCTGGG54150.030.3809643
TATCAAC192150.030.1308882
TCAACGC193450.029.3271964
AACGCAG196650.029.2488336
GTACTAG23500.028.4105641
GTGGTAT37400.027.6573411
GTACATG172100.027.1013031
TACATGG174300.026.0178572
ACGCAGA219450.026.0172737
CGCAGAG227150.025.0937188
ACATGGG173500.024.8699653
TGGTATC42400.024.494472
ATGGGGG107450.023.8785135
CATGGGG140400.023.6692394
Produced by FastQC (version 0.11.5)