##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211190_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5287713 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1834653280161 33.0 31.0 34.0 30.0 34.0 2 32.322365453646974 34.0 31.0 34.0 31.0 34.0 3 32.39977604684672 34.0 31.0 34.0 31.0 34.0 4 36.00816439924028 37.0 35.0 37.0 35.0 37.0 5 35.896251744374176 37.0 35.0 37.0 35.0 37.0 6 35.92268547858025 37.0 35.0 37.0 35.0 37.0 7 35.86624274048157 37.0 35.0 37.0 35.0 37.0 8 35.85418894709301 37.0 35.0 37.0 35.0 37.0 9 37.473066333214376 39.0 37.0 39.0 35.0 39.0 10-11 37.45158511817869 39.0 37.0 39.0 35.0 39.0 12-13 37.356149624610865 39.0 37.0 39.0 34.0 39.0 14-15 38.601043683724896 40.0 38.0 41.0 34.0 41.0 16-17 38.54758418242442 40.0 38.0 41.0 34.0 41.0 18-19 38.56995103554221 40.0 38.0 41.0 34.0 41.0 20-21 38.53085218505619 40.0 38.0 41.0 34.0 41.0 22-23 38.40299861206537 40.0 38.0 41.0 34.0 41.0 24-25 38.34042420607926 40.0 38.0 41.0 34.0 41.0 26-27 38.204416446202735 40.0 38.0 41.0 34.0 41.0 28-29 38.1719543969198 40.0 38.0 41.0 33.5 41.0 30-31 38.17425841758053 40.0 37.5 41.0 34.0 41.0 32-33 38.03106324038389 40.0 37.0 41.0 33.0 41.0 34-35 37.87880885365753 40.0 37.0 41.0 33.0 41.0 36-37 37.726615173705525 40.0 37.0 41.0 33.0 41.0 38-39 37.63673661940427 40.0 37.0 41.0 33.0 41.0 40-41 37.53975404867851 40.0 37.0 41.0 32.5 41.0 42-43 37.39670345194605 40.0 36.0 41.0 32.0 41.0 44-45 37.26574163537242 40.0 36.0 41.0 31.5 41.0 46-47 37.03336754472113 39.5 36.0 41.0 31.0 41.0 48-49 36.81155624369174 39.0 35.5 41.0 30.5 41.0 50-51 36.61894669775005 39.0 35.0 41.0 30.0 41.0 52-53 36.42297010068436 39.0 35.0 40.5 30.0 41.0 54-55 36.19221797779116 39.0 35.0 40.0 29.0 41.0 56-57 35.946901997139406 38.0 35.0 40.0 29.0 41.0 58-59 35.68797966152853 38.0 34.5 40.0 28.0 41.0 60-61 35.42485021407175 37.5 34.0 40.0 28.0 41.0 62-63 35.11964454197874 37.0 34.0 40.0 27.5 41.0 64-65 34.74553393877467 36.5 34.0 39.5 27.0 41.0 66-67 34.439856418077156 36.0 33.5 39.0 26.0 40.5 68-69 34.104187481431005 35.5 33.0 39.0 26.0 40.0 70-71 33.76251377485881 35.0 33.0 38.0 26.0 40.0 72-73 33.41434567269441 35.0 33.0 37.5 26.0 40.0 74-75 33.068793540799206 35.0 32.0 37.0 25.5 39.0 76-77 32.06929177131966 34.0 30.5 36.0 24.5 38.5 78-79 32.55060599922878 35.0 32.0 36.0 25.0 38.0 80-81 32.47417796692067 35.0 32.0 36.0 25.0 37.0 82-83 32.21968041760209 35.0 32.0 35.0 25.0 37.0 84-85 31.963739333053816 34.0 32.0 35.0 25.0 36.5 86-87 31.72375845663333 34.0 31.0 35.0 24.0 36.0 88-89 31.507137395694507 34.0 31.0 35.0 24.0 36.0 90-91 31.29515312196407 34.0 31.0 35.0 23.5 35.0 92-93 31.120275911343903 34.0 31.0 35.0 23.0 35.0 94-95 30.93589411906433 34.0 31.0 35.0 21.5 35.0 96-97 30.767825523056942 34.0 30.0 35.0 20.0 35.0 98-99 30.59280155711931 34.0 30.0 35.0 20.0 35.0 100 28.83680638491537 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.28446102439787424 1101 2 -0.3000174994915099 1101 3 -0.312984389620766 1101 4 -0.1788479119656614 1101 5 -0.22256810811455807 1101 6 -0.3395633523333572 1101 7 -0.35276934732075915 1101 8 -0.36320866802974905 1101 9 -0.47482554117561193 1101 10-11 -0.45273711055809684 1101 12-13 -0.5415962499590492 1101 14-15 -0.6321100586733266 1101 16-17 -0.6441216777237955 1101 18-19 -0.7199792288710327 1101 20-21 -0.6729104334387159 1101 22-23 -0.7264635244672846 1101 24-25 -0.7251935340394269 1101 26-27 -0.8412268260479792 1101 28-29 -0.7888974817435397 1101 30-31 -0.7491669149135021 1101 32-33 -0.6827516973681966 1101 34-35 -0.6882360139723644 1101 36-37 -0.6451988365446439 1101 38-39 -0.6017237146597054 1101 40-41 -0.6391048507499377 1101 42-43 -0.6134558578692193 1101 44-45 -0.6746639725892436 1101 46-47 -0.7205564772594357 1101 48-49 -0.5690604725784354 1101 50-51 -0.7040065909683619 1101 52-53 -0.7644635438400726 1101 54-55 -0.6949250779589349 1101 56-57 -0.6773752745623511 1101 58-59 -0.7163348165978363 1101 60-61 -0.6811323508710458 1101 62-63 -0.8464273018911328 1101 64-65 -0.8113625947431728 1101 66-67 -0.7925301358844266 1101 68-69 -0.8269115024990015 1101 70-71 -0.9698864130119489 1101 72-73 -0.9435887083900241 1101 74-75 -0.9694027414484125 1101 76-77 -0.8333758165848053 1101 78-79 -0.7248966878006051 1101 80-81 -0.6174024952167798 1101 82-83 -0.7298367833741466 1101 84-85 -0.6223392941089436 1101 86-87 -0.8415566893853494 1101 88-89 -0.7673178326275298 1101 90-91 -0.8203044922484253 1101 92-93 -0.8918595178976076 1101 94-95 -0.8235071723024099 1101 96-97 -0.9818060809257894 1101 98-99 -1.064838178491545 1101 100 -0.8932110829501099 1102 1 -0.45589731632650654 1102 2 -0.38576850575510235 1102 3 -0.4539558119415297 1102 4 -0.31351690118679443 1102 5 -0.34025189170701253 1102 6 -0.36102897977111326 1102 7 -0.40330637076149856 1102 8 -0.40115106488508445 1102 9 -0.5236304453205136 1102 10-11 -0.4807553032012635 1102 12-13 -0.4752388508091556 1102 14-15 -0.6942892769241027 1102 16-17 -0.6921078065915012 1102 18-19 -0.7088123145993492 1102 20-21 -0.7532179034437902 1102 22-23 -0.6424417329936745 1102 24-25 -0.7367663160824449 1102 26-27 -0.7150217145705753 1102 28-29 -0.7344930595299886 1102 30-31 -0.7186593392237981 1102 32-33 -0.6356057337402916 1102 34-35 -0.6658037276034463 1102 36-37 -0.8437028092686774 1102 38-39 -0.7901202531582427 1102 40-41 -0.747047733986939 1102 42-43 -0.6637077966761069 1102 44-45 -0.7012262201372295 1102 46-47 -0.8158929682008704 1102 48-49 -0.8392834391855857 1102 50-51 -0.7978358649019626 1102 52-53 -0.7947031419431028 1102 54-55 -0.8313898394601082 1102 56-57 -0.8997676756661477 1102 58-59 -0.9325396602699456 1102 60-61 -0.8347819904710931 1102 62-63 -0.8878926674310677 1102 64-65 -0.8332754176245345 1102 66-67 -0.938527854813529 1102 68-69 -0.9336835498891176 1102 70-71 -0.9836967475982235 1102 72-73 -0.9061688518292854 1102 74-75 -1.0098956882743941 1102 76-77 -0.8413610416096731 1102 78-79 -0.7390804975336138 1102 80-81 -0.7945691310497374 1102 82-83 -0.6735622532222756 1102 84-85 -0.7462732543101218 1102 86-87 -0.8225125090944978 1102 88-89 -0.8719139620731866 1102 90-91 -0.8986448241280947 1102 92-93 -0.9215835415971938 1102 94-95 -0.9623539732058184 1102 96-97 -1.1660696306671277 1102 98-99 -1.1208546506826735 1102 100 -1.0779906255406502 1103 1 -0.365833805488208 1103 2 -0.37100840212310615 1103 3 -0.4063912339860707 1103 4 -0.34702868944625465 1103 5 -0.2973351076295927 1103 6 -0.4098170629489317 1103 7 -0.4920667340904643 1103 8 -0.49906388592274453 1103 9 -0.5329794890172863 1103 10-11 -0.590834790536654 1103 12-13 -0.5321715234992794 1103 14-15 -0.7535938627043777 1103 16-17 -0.7575511595666313 1103 18-19 -0.7664723884137601 1103 20-21 -0.7323292057298829 1103 22-23 -0.7534555453964771 1103 24-25 -0.8822080149847764 1103 26-27 -0.879362160809471 1103 28-29 -0.8736831214913181 1103 30-31 -0.8243400149106606 1103 32-33 -0.6648288013304082 1103 34-35 -0.7465645624750294 1103 36-37 -0.7910228745706505 1103 38-39 -0.8075782333742652 1103 40-41 -0.788806007730237 1103 42-43 -0.7691860227056324 1103 44-45 -0.8657442013262937 1103 46-47 -0.9550360737103247 1103 48-49 -1.0056902754421202 1103 50-51 -1.0549721505637208 1103 52-53 -1.1177794969487636 1103 54-55 -0.9446849959892276 1103 56-57 -0.9349148860266681 1103 58-59 -0.9764533152202759 1103 60-61 -0.9862943532288497 1103 62-63 -0.8069002529467966 1103 64-65 -0.863662741473675 1103 66-67 -1.0182818811879812 1103 68-69 -0.9454129371724136 1103 70-71 -1.0060330651545826 1103 72-73 -0.9701799623757523 1103 74-75 -0.9789295566867793 1103 76-77 -0.9518572262448934 1103 78-79 -0.8960907449197002 1103 80-81 -0.768163450571862 1103 82-83 -0.8351121816125087 1103 84-85 -0.9732438816600961 1103 86-87 -0.9853578374150018 1103 88-89 -0.9399234592392922 1103 90-91 -1.0257895906808194 1103 92-93 -1.0873093209106308 1103 94-95 -1.0107754239868605 1103 96-97 -1.1537353905923027 1103 98-99 -1.172988063146498 1103 100 -1.180246423850022 1104 1 -0.3939290589196638 1104 2 -0.382046249543631 1104 3 -0.4012378993144772 1104 4 -0.29097277095246454 1104 5 -0.35211481547310086 1104 6 -0.35942263942723685 1104 7 -0.3937798491460427 1104 8 -0.45131566553597935 1104 9 -0.5687361236017239 1104 10-11 -0.5518299878088087 1104 12-13 -0.6556860649902845 1104 14-15 -0.7624784105871854 1104 16-17 -0.8056993251665077 1104 18-19 -0.8110899653070831 1104 20-21 -0.7915625442661991 1104 22-23 -0.8006800285487756 1104 24-25 -0.8543278650582735 1104 26-27 -0.9364591262854915 1104 28-29 -0.9775368961232331 1104 30-31 -0.8012806401216679 1104 32-33 -0.8506262607484913 1104 34-35 -0.8396227441760971 1104 36-37 -0.8430426973970881 1104 38-39 -0.8826989923538591 1104 40-41 -0.818155558885806 1104 42-43 -0.7814383448810887 1104 44-45 -0.8727145476017597 1104 46-47 -0.8787588932186239 1104 48-49 -0.87323670110991 1104 50-51 -0.7832157943054767 1104 52-53 -0.7667594433732603 1104 54-55 -0.8162529010823789 1104 56-57 -0.8726116105794475 1104 58-59 -0.8390826220709684 1104 60-61 -1.0159590342214813 1104 62-63 -0.9578224211608486 1104 64-65 -0.9978036568685482 1104 66-67 -0.9448172179086853 1104 68-69 -0.8842070921761263 1104 70-71 -1.0347891602876018 1104 72-73 -0.9805937946199066 1104 74-75 -0.9697900137089874 1104 76-77 -0.8587692859666269 1104 78-79 -0.7940699019298876 1104 80-81 -0.7162640802185223 1104 82-83 -0.7841955305151522 1104 84-85 -0.793537255026532 1104 86-87 -0.8850952437040718 1104 88-89 -0.9498244878384519 1104 90-91 -0.8572638173283664 1104 92-93 -0.8880092216025304 1104 94-95 -0.9112200596750846 1104 96-97 -0.8695495331125116 1104 98-99 -0.9654352539093622 1104 100 -1.0328226611617985 1105 1 -0.4110478021350801 1105 2 -0.36366673152186735 1105 3 -0.44148436517442846 1105 4 -0.2242796574597108 1105 5 -0.28305090106088215 1105 6 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1105 68-69 -0.7553218446333005 1105 70-71 -0.7563886016920947 1105 72-73 -0.827350699034973 1105 74-75 -0.7578902848335716 1105 76-77 -0.8211802621489603 1105 78-79 -0.7533894145659232 1105 80-81 -0.7339063171203399 1105 82-83 -0.6838852576720633 1105 84-85 -0.7677394201328873 1105 86-87 -0.7736754747551089 1105 88-89 -0.8826842141941036 1105 90-91 -0.7721906950664028 1105 92-93 -0.8576695413878426 1105 94-95 -0.8421460626334927 1105 96-97 -0.9892749572387629 1105 98-99 -0.9232353678341525 1105 100 -0.9188327362961246 1106 1 -0.4063408012218339 1106 2 -0.3881073438902547 1106 3 -0.37188214388718066 1106 4 -0.28219338834130525 1106 5 -0.29600147452250525 1106 6 -0.29407239186623 1106 7 -0.35548504157956273 1106 8 -0.3670121078840509 1106 9 -0.4885219613540457 1106 10-11 -0.40147183001429454 1106 12-13 -0.5202059821769822 1106 14-15 -0.5852445020529231 1106 16-17 -0.6436496469469901 1106 18-19 -0.5670590177392327 1106 20-21 -0.6220667484088196 1106 22-23 -0.589080550038247 1106 24-25 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0.9818176560947975 2311 26-27 0.39344531197500743 2311 28-29 0.6931059441833796 2311 30-31 0.826941358839683 2311 32-33 0.8608297675902179 2311 34-35 0.9138383770638896 2311 36-37 0.9819445047991522 2311 38-39 0.9699661631492376 2311 40-41 0.9593645582733004 2311 42-43 0.9389341902976014 2311 44-45 0.8612997119452501 2311 46-47 0.5164409762842723 2311 48-49 0.7952775648205588 2311 50-51 0.939174085209153 2311 52-53 0.8733892747254046 2311 54-55 0.9223240552107015 2311 56-57 0.9780787054950721 2311 58-59 0.9704448572057842 2311 60-61 0.9933310421919472 2311 62-63 0.9987269415709861 2311 64-65 1.0094414650369643 2311 66-67 1.0607878014386856 2311 68-69 1.052502482997184 2311 70-71 1.0691681135118145 2311 72-73 1.1238683214785894 2311 74-75 1.119547356578117 2311 76-77 1.037939567807289 2311 78-79 0.776140740516432 2311 80-81 0.806947717565059 2311 82-83 0.8995505757470497 2311 84-85 0.9675379073775652 2311 86-87 0.97512193732517 2311 88-89 1.0284824969138704 2311 90-91 1.0555564726783082 2311 92-93 1.0856410133567742 2311 94-95 1.109445287469029 2311 96-97 1.1945999644544294 2311 98-99 1.2330045431488372 2311 100 1.3465315607681134 2312 1 0.21120665346970213 2312 2 0.3453432899896285 2312 3 0.4194062643707497 2312 4 0.1789920375744103 2312 5 0.3333006822800044 2312 6 0.4158084698675424 2312 7 0.3098835813080214 2312 8 0.4188402188324929 2312 9 0.5862685936339673 2312 10-11 0.5980379390834045 2312 12-13 0.6616326780419968 2312 14-15 0.8414966706390317 2312 16-17 0.8115355408322742 2312 18-19 0.7754138525487733 2312 20-21 0.7934732560137121 2312 22-23 0.8954930106120145 2312 24-25 0.8990303348912292 2312 26-27 0.9467906802929278 2312 28-29 0.9181840599285138 2312 30-31 0.9380482581875285 2312 32-33 0.7672969704779504 2312 34-35 0.8919021731061747 2312 36-37 0.9297624727691556 2312 38-39 0.796436032856711 2312 40-41 0.8842503262284183 2312 42-43 0.9436242035760003 2312 44-45 0.6085934907359771 2312 46-47 0.718565889068536 2312 48-49 0.9138299376067849 2312 50-51 0.7459612033197729 2312 52-53 0.8741567525415803 2312 54-55 0.7458289510244853 2312 56-57 0.8520171799885716 2312 58-59 1.0129743787867866 2312 60-61 0.9461424380062198 2312 62-63 0.9716495155579494 2312 64-65 1.0053421386629395 2312 66-67 0.9732478964859723 2312 68-69 1.0591427919336311 2312 70-71 0.9608854979738197 2312 72-73 0.9576000790210912 2312 74-75 1.0163134289374796 2312 76-77 1.0186141112620426 2312 78-79 0.9324253763598946 2312 80-81 0.879265772873282 2312 82-83 0.863017917416677 2312 84-85 0.9072649191052271 2312 86-87 0.9446929878030517 2312 88-89 1.0092636280631773 2312 90-91 1.0504671068837332 2312 92-93 1.0638220597310202 2312 94-95 1.1373767559264216 2312 96-97 0.7762346764732264 2312 98-99 0.5822930628741005 2312 100 0.8806938258413304 2313 1 0.07998426514414092 2313 2 0.2678019339652522 2313 3 0.30433797666373863 2313 4 0.20194174833620337 2313 5 0.3033214842686007 2313 6 0.21796617025999865 2313 7 0.2548481756502099 2313 8 0.35665540692899356 2313 9 0.6014675499680209 2313 10-11 0.3952889789323706 2313 12-13 0.4158554897407143 2313 14-15 0.4756533425532794 2313 16-17 0.7396664184409687 2313 18-19 0.6583888665192816 2313 20-21 0.6575394637222303 2313 22-23 0.6637989659476418 2313 24-25 0.4783081871049504 2313 26-27 0.7408335614024111 2313 28-29 0.6178581547604907 2313 30-31 0.8031223314601377 2313 32-33 0.8894655998288741 2313 34-35 0.950604825890693 2313 36-37 0.9438660036372326 2313 38-39 0.9139404444130932 2313 40-41 0.877589712615837 2313 42-43 0.9635364172606344 2313 44-45 0.8820404846362351 2313 46-47 0.9311228856557037 2313 48-49 1.0144810967536202 2313 50-51 0.9113036516990078 2313 52-53 0.9181784700020046 2313 54-55 0.8827963058077941 2313 56-57 0.6576641524897582 2313 58-59 0.7441864432589256 2313 60-61 0.7066605962668362 2313 62-63 1.0110523981020592 2313 64-65 1.147551566520093 2313 66-67 1.1144528149079989 2313 68-69 1.2220822519755643 2313 70-71 1.1966652539642126 2313 72-73 1.1793094647012268 2313 74-75 1.2365240776241109 2313 76-77 1.1547440617776736 2313 78-79 0.996353672213786 2313 80-81 0.9199726868395643 2313 82-83 1.0077212746877322 2313 84-85 0.9869670461296032 2313 86-87 1.0672575652343568 2313 88-89 1.0316500601654788 2313 90-91 0.85733257961002 2313 92-93 1.1311479101991502 2313 94-95 1.1818794767952703 2313 96-97 1.2801614475425538 2313 98-99 1.2521405383337836 2313 100 1.3636786527321973 2314 1 -0.4076812974875601 2314 2 0.06208328122880857 2314 3 0.3047693392435278 2314 4 0.22533652679140204 2314 5 0.32717056312747417 2314 6 0.3685705161740742 2314 7 0.4369416733371878 2314 8 0.4523644511035414 2314 9 0.3545762582081551 2314 10-11 0.54380761433152 2314 12-13 0.478715524697769 2314 14-15 0.7524946507981909 2314 16-17 0.7668620941498077 2314 18-19 0.8263692593063823 2314 20-21 0.8093471200853841 2314 22-23 0.8617313597555025 2314 24-25 0.7300473100229468 2314 26-27 0.6495173540035708 2314 28-29 0.6935692151259332 2314 30-31 0.7164835042988287 2314 32-33 0.7635630135040046 2314 34-35 0.7607935783701407 2314 36-37 0.7298624743291668 2314 38-39 0.49668578294566856 2314 40-41 0.5551572513581178 2314 42-43 0.725376376508251 2314 44-45 0.6815992724282509 2314 46-47 0.6280290675209912 2314 48-49 0.7167164875269663 2314 50-51 0.7035751391073077 2314 52-53 0.7147383686470121 2314 54-55 0.6835008646881136 2314 56-57 0.5888949250171578 2314 58-59 0.7515939115416614 2314 60-61 0.751427923455168 2314 62-63 0.7529483943073316 2314 64-65 0.7699510455246781 2314 66-67 0.47428883138232436 2314 68-69 0.4621841913661342 2314 70-71 0.6249201603939625 2314 72-73 0.7493401507369555 2314 74-75 0.8356594531542711 2314 76-77 0.6471233959184595 2314 78-79 0.5261675592885879 2314 80-81 0.5916818883159607 2314 82-83 0.6691355936364687 2314 84-85 0.735573816829767 2314 86-87 0.7414283336806733 2314 88-89 0.7002507847279951 2314 90-91 0.8116518424633661 2314 92-93 0.7596021102802801 2314 94-95 0.734869721645584 2314 96-97 0.7196035714194657 2314 98-99 0.8295836235538872 2314 100 0.8896779699545121 2315 1 0.4241524371012204 2315 2 0.44003788389233023 2315 3 0.44428673368511085 2315 4 0.2899368218959282 2315 5 0.33118586114633075 2315 6 0.392344331442672 2315 7 0.3426980112575464 2315 8 0.18658860912100295 2315 9 0.4123918128417259 2315 10-11 0.5321705065094235 2315 12-13 0.35006031874730326 2315 14-15 0.541562417575868 2315 16-17 0.7415613805286156 2315 18-19 0.7421358027848726 2315 20-21 0.46345816270084583 2315 22-23 0.8611407667701059 2315 24-25 0.8819667612216406 2315 26-27 0.828033418339146 2315 28-29 0.7840552737288249 2315 30-31 0.846704566334374 2315 32-33 0.8071158507235552 2315 34-35 0.8627885814140512 2315 36-37 0.8755449689294323 2315 38-39 0.9087968025248401 2315 40-41 0.8773794282542937 2315 42-43 1.0063705941585184 2315 44-45 0.9876777705194826 2315 46-47 0.9604007355670419 2315 48-49 0.8635361471801204 2315 50-51 0.8573572666386227 2315 52-53 0.9178297193105251 2315 54-55 0.7548167433895472 2315 56-57 0.7801076587125593 2315 58-59 0.8285140886235709 2315 60-61 0.8985587650453724 2315 62-63 0.9916132059124365 2315 64-65 1.0138739872018263 2315 66-67 1.0159670661879119 2315 68-69 0.9829387403742942 2315 70-71 0.8540505019046165 2315 72-73 0.9413867382819561 2315 74-75 0.4188215420374135 2315 76-77 0.45213139023232785 2315 78-79 0.7028270423109646 2315 80-81 0.7694531844698389 2315 82-83 0.6651424680505116 2315 84-85 0.7044469725164824 2315 86-87 0.8089631286828549 2315 88-89 0.770232862011401 2315 90-91 0.8001816637857111 2315 92-93 0.8381059245866318 2315 94-95 0.8761413058731158 2315 96-97 0.9378190409147322 2315 98-99 0.9424623996163959 2315 100 0.9634866396157378 2316 1 0.2613250557743001 2316 2 0.03858304711380356 2316 3 0.18478296608960676 2316 4 -0.036978931135415394 2316 5 0.2508661106231571 2316 6 0.34296998685820057 2316 7 0.4253443065317768 2316 8 0.4165145970190096 2316 9 0.5836086548362331 2316 10-11 0.6026261321284139 2316 12-13 0.36177453330792986 2316 14-15 0.5109720702693892 2316 16-17 0.774882746107302 2316 18-19 0.7230554581021877 2316 20-21 0.7581943439655419 2316 22-23 0.7940636151076887 2316 24-25 0.44117885560203973 2316 26-27 0.7201337730003772 2316 28-29 0.888322274224727 2316 30-31 0.5452164283205079 2316 32-33 0.6853388298154499 2316 34-35 0.7871885086707948 2316 36-37 0.8399436112571337 2316 38-39 0.7674873699440141 2316 40-41 0.7472572048198955 2316 42-43 0.7450125026963619 2316 44-45 0.6410279012879769 2316 46-47 0.7435639924250168 2316 48-49 0.8709557371628591 2316 50-51 0.9658771173257108 2316 52-53 0.9128734225100814 2316 54-55 0.7868960187804035 2316 56-57 0.9140889034795308 2316 58-59 0.7579270369046114 2316 60-61 0.9274273263610482 2316 62-63 0.9786051444493111 2316 64-65 0.9605374626775145 2316 66-67 0.9395192525211655 2316 68-69 0.9276316049589468 2316 70-71 0.8368626599448348 2316 72-73 0.8789772238271141 2316 74-75 0.8816544727817259 2316 76-77 0.7050513867465575 2316 78-79 0.5239101353035878 2316 80-81 0.4785858176272271 2316 82-83 0.4372624552542064 2316 84-85 0.5719861876897703 2316 86-87 0.596103846485093 2316 88-89 0.5933292155095735 2316 90-91 0.47710979301134415 2316 92-93 0.36619380635272236 2316 94-95 0.41492312312422186 2316 96-97 0.6298954474507603 2316 98-99 0.7640677197524894 2316 100 0.3576378659234365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 4.0 12 16.0 13 121.0 14 495.0 15 1465.0 16 3382.0 17 7255.0 18 12326.0 19 17460.0 20 24131.0 21 32396.0 22 39667.0 23 42118.0 24 42877.0 25 46196.0 26 52836.0 27 63883.0 28 78167.0 29 96668.0 30 118970.0 31 147873.0 32 187883.0 33 246993.0 34 350223.0 35 493603.0 36 766983.0 37 1191336.0 38 1056721.0 39 165648.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.343308059737865 19.38453364937626 13.378999114156507 23.893159176729373 2 15.895094911543042 19.946203585557686 40.41276445979576 23.745937043103513 3 19.115636571046878 25.277109404387115 29.771282972430612 25.835971052135392 4 12.781180697396422 16.353328804514895 37.710302040408635 33.15518845768005 5 13.239201900708302 37.16722144337259 34.47085724962758 15.12271940629153 6 31.098454095371665 36.36984458120175 18.096878556003322 14.434822767423269 7 27.608351414675514 32.13720046167704 22.055196618402896 18.19925150524455 8 26.129349304699407 35.58128060278612 19.851796041124018 18.43757405139046 9 26.417206282773215 16.107625893402272 20.707243225576555 36.76792459824796 10-11 25.56812825254116 26.01301040103977 27.671247902558633 20.747613443860434 12-13 25.66829229085356 23.604970958613958 28.042674407884316 22.68406234264817 14-15 22.923447332359252 25.10373118323901 25.973579090819342 25.999242393582396 16-17 22.307989368538543 27.648135239088738 26.92851261285148 23.115362779521238 18-19 21.895997381098407 27.033010301428995 29.06273468321749 22.008257634255113 20-21 22.88007770469323 26.372278442195647 28.87261341697632 21.8750304361348 22-23 22.812007762145942 26.156430956067396 28.635177438714997 22.396383843071664 24-25 22.392042342147175 26.087599936191374 29.082851262078414 22.437506459583034 26-27 22.37913486245698 26.29538110301831 28.67068064302547 22.65480339149924 28-29 22.055010154232566 26.79665899886652 28.783894839652767 22.36443600724815 30-31 22.85955065795278 26.344692383115852 28.838954639985754 21.956802318945613 32-33 22.791979735603498 26.42524704420807 28.494604029425137 22.288169190763295 34-35 22.791819425667146 26.556675772461936 28.66673148736838 21.98477331450254 36-37 22.647070118410525 26.43936428759118 28.495340894924254 22.418224699074045 38-39 22.572054308201466 26.651023877873133 28.560391164929687 22.216530648995718 40-41 22.660128520583537 26.085449725118153 28.86345891277869 22.390962841519617 42-43 22.48941625858985 26.396949378554176 28.399867655361927 22.71376670749405 44-45 22.337940666879827 26.843971319327398 28.447237146084127 22.370850867708654 46-47 22.384734434098238 26.485090645515918 28.067462266028194 23.06271265435765 48-49 22.666708652279784 26.517545872828414 28.090427176190452 22.72531829870135 50-51 22.57833399232867 26.69593104030697 27.95262458723738 22.773110380126983 52-53 22.453601143987978 26.956346826726126 27.66093667089172 22.92911535839418 54-55 22.309306600319587 26.864997379020494 27.85313927903062 22.972556741629298 56-57 22.69910439169006 26.71317215775279 27.512207124054715 23.075516326502438 58-59 22.260839270814884 26.92064836419046 27.895853880031463 22.9226584849632 60-61 22.60531068851715 27.102544704914465 27.657018033267455 22.63512657330093 62-63 22.644582819674312 26.867651902253854 27.504940535330018 22.982824742741816 64-65 22.269955833691917 26.728661503856593 27.61824970654959 23.383132955901896 66-67 22.34900868127701 26.655660845086626 27.567974573070252 23.42735590056611 68-69 22.099672816109027 26.75549579997313 27.699191407307044 23.4456399766108 70-71 22.181725361780117 26.51423137087129 27.74882216514986 23.555221102198733 72-73 22.311525101085387 26.79563794360523 27.57179661660889 23.321040338700495 74-75 22.54766303335064 26.910801665971405 27.282741228630265 23.258794072047685 76-77 22.355765501767873 26.797868335474206 27.207667030520184 23.638699132237736 78-79 22.173097572845744 26.817027247204457 27.365648355526908 23.644226824422898 80-81 22.029747605388213 27.106471160879046 27.328819357928687 23.534961875804054 82-83 22.42656942708346 26.778531413136612 27.693813368639063 23.101085791140864 84-85 22.557953375422297 27.015412637077613 27.110172885418343 23.316461102081746 86-87 21.998910796999127 27.281621042887274 27.295756460567105 23.423711699546494 88-89 22.17042349387438 26.734767705792308 27.327964396086347 23.766844404246964 90-91 22.38010620225201 26.605613947652312 27.3888340334614 23.625445816634276 92-93 22.31029597327302 26.801852317302753 27.32404513360888 23.563806575815345 94-95 21.834284059556524 26.78890775903436 27.46375031184917 23.913057869559946 96-97 21.807528747245986 26.786567543699473 27.349034937356574 24.056868771697964 98-99 21.58487246126766 26.96000176295767 27.223518536260887 24.23160723951378 100 21.679899767560777 26.83512440993978 27.176206789374348 24.308769033125095 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1294.0 1 964.0 2 1617.5 3 2371.5 4 1577.5 5 1119.5 6 1616.0 7 2841.5 8 4313.5 9 4956.5 10 4318.0 11 3627.5 12 3801.5 13 4277.0 14 4763.5 15 5380.5 16 6083.0 17 6686.0 18 7311.5 19 8065.0 20 8907.0 21 9829.0 22 11133.5 23 12906.0 24 15307.0 25 18509.0 26 22588.0 27 27523.0 28 33514.0 29 41460.5 30 51755.5 31 62432.0 32 74284.0 33 86378.5 34 97114.0 35 108079.5 36 119310.5 37 135048.0 38 145518.5 39 147036.5 40 147655.5 41 148156.5 42 152238.5 43 160526.0 44 170940.5 45 186356.5 46 200532.0 47 211147.5 48 221215.0 49 225453.0 50 223510.5 51 221724.0 52 218395.5 53 209854.0 54 200055.0 55 182368.5 56 162338.0 57 142082.5 58 122510.5 59 107448.5 60 87077.0 61 67240.0 62 53105.5 63 41827.0 64 31237.0 65 21511.5 66 15464.0 67 11770.5 68 9038.5 69 6983.5 70 5604.5 71 4354.0 72 3429.5 73 2758.5 74 2134.0 75 1562.0 76 1126.5 77 760.5 78 468.0 79 290.0 80 179.5 81 112.0 82 67.0 83 44.0 84 34.0 85 24.0 86 10.5 87 6.0 88 6.0 89 3.5 90 2.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044537212969009476 2 0.0 3 0.0 4 0.029615828241812667 5 0.0 6 0.0 7 6.430001023126633E-4 8 0.0 9 0.011744207751063645 10-11 3.876912381591059E-4 12-13 9.455883857539167E-6 14-15 1.3238237400554833E-4 16-17 2.080294448658617E-4 18-19 0.0 20-21 2.8367651572617504E-5 22-23 0.0 24-25 5.011618444495758E-4 26-27 4.160588897317234E-4 28-29 3.1204416729879253E-4 30-31 0.002600368060823271 32-33 0.015223973010638058 34-35 0.025266121667344654 36-37 0.030485769556706274 38-39 0.021653974033764692 40-41 0.0014656619979185707 42-43 6.619118700277416E-5 44-45 0.011318692977474383 46-47 0.018249855845050594 48-49 0.0035459564465771874 50-51 0.01181039893806642 52-53 0.01124304590661407 54-55 0.010732428178306955 56-57 4.160588897317234E-4 58-59 0.0088601631745142 60-61 0.004973794909065602 62-63 0.0014656619979185707 64-65 0.03586616747164606 66-67 0.061217392093708564 68-69 0.06095262734569747 70-71 0.053028596673079645 72-73 0.04640002208894469 74-75 0.03608365280036946 76-77 0.032206740418778405 78-79 0.01554547306179439 80-81 0.0011441619467622392 82-83 0.039223006241072465 84-85 0.05288675841521656 86-87 0.05891015643246901 88-89 0.07185526143344013 90-91 0.04933134608478183 92-93 0.04004566813667837 94-95 0.01327606093598499 96-97 0.0039052800331636755 98-99 0.021606694614476996 100 0.030258828344125334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5287713.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.5651946200192 #Duplication Level Percentage of deduplicated Percentage of total 1 79.05727800407028 53.41520374473971 2 13.456699829962284 18.184090859091622 3 3.7949785408755243 7.69225391079154 4 1.441946647462229 3.8970162346987887 5 0.6955453608454996 2.349732883628883 6 0.4165495029622556 1.6886548941910242 7 0.2567784398396756 1.21444996833948 8 0.18097555113352823 0.9782118667041656 9 0.12691473103027173 0.7717516651986929 >10 0.5412237932429511 6.126644547924936 >50 0.021248045722015777 0.9716180709335517 >100 0.008747908291671968 1.0665648260876288 >500 6.55085341492618E-4 0.2953755145516257 >1k 3.6029653021795176E-4 0.46947556272371094 >5k 3.2754230019741906E-5 0.1990856114951098 >10k+ 6.550846003948381E-5 0.6798698388996519 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16742 0.31662081508584144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13979 0.26436760088908 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8996 0.1701302623648447 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.8911767715078333E-5 0.0 5 0.0 3.782353543015667E-5 0.0 1.8911767715078333E-5 0.0 6 3.782353543015667E-5 3.782353543015667E-5 0.0 1.8911767715078333E-5 0.0 7 3.782353543015667E-5 3.782353543015667E-5 0.0 1.8911767715078333E-5 0.0 8 3.782353543015667E-5 3.782353543015667E-5 0.0 3.782353543015667E-5 0.0 9 3.782353543015667E-5 5.6735303145235E-5 0.0 3.782353543015667E-5 0.0 10-11 3.782353543015667E-5 5.6735303145235E-5 0.0 3.782353543015667E-5 0.0 12-13 4.727941928769584E-5 5.6735303145235E-5 0.0 3.782353543015667E-5 0.0 14-15 5.6735303145235E-5 5.6735303145235E-5 0.0 5.6735303145235E-5 3.782353543015667E-5 16-17 5.6735303145235E-5 5.6735303145235E-5 0.0 1.1347060629047E-4 5.6735303145235E-5 18-19 5.6735303145235E-5 5.6735303145235E-5 0.0 1.418382578630875E-4 8.51029547178525E-5 20-21 5.6735303145235E-5 5.6735303145235E-5 0.0 1.70205909435705E-4 9.455883857539167E-5 22-23 5.6735303145235E-5 5.6735303145235E-5 0.0 3.97147122016645E-4 1.2292649014800918E-4 24-25 5.6735303145235E-5 5.6735303145235E-5 0.0 8.037501278908292E-4 1.6075002557816584E-4 26-27 5.6735303145235E-5 5.6735303145235E-5 0.0 0.00170205909435705 1.70205909435705E-4 28-29 5.6735303145235E-5 5.6735303145235E-5 0.0 0.004813044883487436 1.70205909435705E-4 30-31 5.6735303145235E-5 5.6735303145235E-5 0.0 0.0158858848806658 1.70205909435705E-4 32-33 5.6735303145235E-5 5.6735303145235E-5 0.0 0.04240018321720562 1.8911767715078335E-4 34-35 5.6735303145235E-5 5.6735303145235E-5 0.0 0.08456396933797278 2.458529802960183E-4 36-37 5.6735303145235E-5 5.6735303145235E-5 0.0 0.1487694207306637 2.458529802960183E-4 38-39 5.6735303145235E-5 6.619118700277416E-5 0.0 0.2479427306285345 2.458529802960183E-4 40-41 5.6735303145235E-5 7.564707086031333E-5 0.0 0.36234001353704337 2.458529802960183E-4 42-43 5.6735303145235E-5 9.455883857539167E-5 0.0 0.46892673637922483 2.553088641535575E-4 44-45 7.564707086031333E-5 1.1347060629047E-4 0.0 0.5950020358517946 2.6476474801109665E-4 46-47 9.455883857539167E-5 1.1347060629047E-4 0.0 0.7391758970276943 2.6476474801109665E-4 48-49 9.455883857539167E-5 1.418382578630875E-4 0.0 0.892559789080837 2.6476474801109665E-4 50-51 9.455883857539167E-5 1.5129414172062667E-4 0.0 1.0526857263244054 2.83676515726175E-4 52-53 9.455883857539167E-5 1.5129414172062667E-4 0.0 1.2093413541922566 2.931323995837142E-4 54-55 9.455883857539167E-5 1.5129414172062667E-4 0.0 1.3883979709186183 3.0258828344125333E-4 56-57 9.455883857539167E-5 1.70205909435705E-4 0.0 1.590186532438504 3.0258828344125333E-4 58-59 9.455883857539167E-5 1.70205909435705E-4 0.0 1.820702069117594 3.0258828344125333E-4 60-61 9.455883857539167E-5 1.70205909435705E-4 0.0 2.0502909291786446 3.0258828344125333E-4 62-63 9.455883857539167E-5 1.7966179329324418E-4 0.0 2.2806268040644415 3.0258828344125333E-4 64-65 1.1347060629047E-4 1.8911767715078335E-4 0.0 2.5133644734500526 3.120441672987925E-4 66-67 1.1347060629047E-4 1.8911767715078335E-4 0.0 2.7806728542188277 3.3095593501387087E-4 68-69 1.1347060629047E-4 1.985735610083225E-4 0.0 3.086542329358647 3.4041181887141E-4 70-71 1.1347060629047E-4 2.7422063186863585E-4 0.0 3.4090163365523054 3.4041181887141E-4 72-73 1.1347060629047E-4 3.215000511563317E-4 0.0 3.711387134664835 3.97147122016645E-4 74-75 1.1347060629047E-4 3.215000511563317E-4 0.0 4.01024034398236 3.97147122016645E-4 76-77 1.1347060629047E-4 3.4041181887141E-4 0.0 4.349281059694428 3.97147122016645E-4 78-79 1.1347060629047E-4 3.4041181887141E-4 0.0 4.728783502432904 3.97147122016645E-4 80-81 1.1347060629047E-4 3.4041181887141E-4 0.0 5.11386491664733 4.3497065744680166E-4 82-83 1.1347060629047E-4 3.4041181887141E-4 0.0 5.499967566318369 4.7279419287695834E-4 84-85 1.1347060629047E-4 3.4041181887141E-4 0.0 5.886041848337836 4.7279419287695834E-4 86-87 1.1347060629047E-4 3.4041181887141E-4 0.0 6.2914061334266815 4.822500767344975E-4 88 1.1347060629047E-4 3.4041181887141E-4 0.0 6.623733171599896 4.917059605920366E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8805 0.0 47.52477 1 GTATCAA 14870 0.0 39.175964 1 TACCTGG 4500 0.0 37.699566 2 ATCAACG 18090 0.0 31.231863 3 CAACGCA 18135 0.0 31.146408 5 GTACCTG 5575 0.0 30.95142 1 ACCTGGG 5415 0.0 30.380964 3 TATCAAC 19215 0.0 30.130888 2 TCAACGC 19345 0.0 29.327196 4 AACGCAG 19665 0.0 29.248833 6 GTACTAG 2350 0.0 28.410564 1 GTGGTAT 3740 0.0 27.657341 1 GTACATG 17210 0.0 27.101303 1 TACATGG 17430 0.0 26.017857 2 ACGCAGA 21945 0.0 26.017273 7 CGCAGAG 22715 0.0 25.093718 8 ACATGGG 17350 0.0 24.869965 3 TGGTATC 4240 0.0 24.49447 2 ATGGGGG 10745 0.0 23.878513 5 CATGGGG 14040 0.0 23.669239 4 >>END_MODULE