FastQCFastQC Report
Mon 6 Jun 2016
ERR1211189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9521822
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343660.36091832004421004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271710.2853550507455401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166250.17459893705217341No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA197750.046.593191
GTATCAA348950.039.8088341
GTACTAG94950.038.260321
GCATCGT92200.036.808928
CAACGCA392750.033.6688085
ATCAACG397850.033.22543
GGGCACC96650.033.06715894
TACCTGG48750.031.9504762
TAGGCAT123300.031.7582195
TATCAAC437200.031.6332192
GTGGTAT61750.030.7872311
AACGCAG446050.030.436026
CTGTGCG122050.029.7703539
CTAGGCA135400.029.3713324
TCAACGC469100.028.649744
ACCTGGG53300.028.398993
TAGTACT129800.028.1396224
ACGCAGA468800.028.096817
CGCAGAG470150.027.9942258
CATCGTC123600.027.8758289
Produced by FastQC (version 0.11.5)