##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211189_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9521822 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.522553141615123 31.0 31.0 34.0 30.0 34.0 2 31.540521656464488 31.0 31.0 34.0 28.0 34.0 3 31.603503090059863 33.0 31.0 34.0 28.0 34.0 4 35.20536510764431 37.0 35.0 37.0 32.0 37.0 5 35.27058151265587 37.0 35.0 37.0 33.0 37.0 6 35.32983403806541 37.0 35.0 37.0 32.0 37.0 7 35.24886098479892 37.0 35.0 37.0 32.0 37.0 8 35.256386855372845 37.0 35.0 37.0 32.0 37.0 9 36.82581989035292 39.0 37.0 39.0 32.0 39.0 10-11 36.784063963808606 39.0 37.0 39.0 32.0 39.0 12-13 36.67172506480377 39.0 37.0 39.0 32.0 39.0 14-15 37.86190164025331 40.0 38.0 41.0 32.0 41.0 16-17 37.84840081026509 40.0 37.5 41.0 32.0 41.0 18-19 37.875065034822114 40.0 38.0 41.0 32.0 41.0 20-21 37.84671520849686 40.0 37.5 41.0 32.0 41.0 22-23 37.7310544137456 40.0 37.0 41.0 32.0 41.0 24-25 37.63551975661801 40.0 37.0 41.0 32.0 41.0 26-27 37.47952802520358 40.0 37.0 41.0 31.5 41.0 28-29 37.3694427390052 40.0 36.5 41.0 31.0 41.0 30-31 37.21817037747607 40.0 36.0 41.0 31.0 41.0 32-33 37.033114408145835 39.0 36.0 41.0 30.5 41.0 34-35 36.88894005790068 39.0 35.5 41.0 30.0 41.0 36-37 36.78384063470206 39.0 35.0 41.0 30.0 41.0 38-39 36.632148132993876 39.0 35.0 41.0 30.0 41.0 40-41 36.45926646181792 39.0 35.0 41.0 30.0 41.0 42-43 36.243518887456624 39.0 35.0 40.0 29.0 41.0 44-45 35.99082990629314 38.5 35.0 40.0 28.0 41.0 46-47 35.72762859881229 38.0 35.0 40.0 27.0 41.0 48-49 35.49146833452673 38.0 34.0 40.0 26.5 41.0 50-51 34.78397947367637 37.5 33.5 39.5 25.5 40.5 52-53 34.86922749658626 38.0 33.5 39.5 26.0 40.5 54-55 35.330738119238106 38.0 34.0 40.0 26.5 41.0 56-57 35.26328973593499 38.0 34.0 40.0 26.0 41.0 58-59 35.028462514842225 37.5 34.0 40.0 26.0 41.0 60-61 34.80546118169401 37.0 34.0 40.0 26.0 41.0 62-63 34.500011027301284 36.5 33.5 40.0 26.0 41.0 64-65 34.250653971477306 36.0 33.0 39.5 26.0 41.0 66-67 34.08570770384071 35.5 33.0 39.0 25.5 41.0 68-69 33.81069043298646 35.0 33.0 39.0 25.0 41.0 70-71 33.55016602914862 35.0 33.0 38.5 25.0 41.0 72-73 33.24738579444144 35.0 33.0 37.5 24.0 40.0 74-75 32.93539655540715 35.0 32.0 37.0 24.0 39.5 76-77 32.63368234566872 35.0 32.0 36.5 23.5 39.0 78-79 32.31740023075416 35.0 32.0 36.0 23.0 39.0 80-81 32.042786244061276 35.0 31.0 36.0 22.5 38.5 82-83 31.75904406740643 35.0 31.0 35.0 21.5 37.0 84-85 31.526129925554166 35.0 31.0 35.0 20.0 37.0 86-87 31.306876614580695 34.5 31.0 35.0 20.0 36.5 88-89 31.106345665776992 34.0 31.0 35.0 20.0 36.0 90-91 30.88300448170529 34.0 30.5 35.0 19.5 36.0 92-93 30.683242660910906 34.0 30.0 35.0 19.0 36.0 94-95 30.522857337597785 34.0 30.0 35.0 18.5 35.0 96-97 30.345893096930396 34.0 29.5 35.0 18.0 35.0 98-99 30.121177333497727 34.0 29.0 35.0 17.5 35.0 100 28.961933650933613 33.0 27.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.47558694705668003 1101 2 -0.4913252177117684 1101 3 -0.45491025551289965 1101 4 -0.3874380839579743 1101 5 -0.3682440769982662 1101 6 -0.33300695337540986 1101 7 -0.3589148585123141 1101 8 -0.33371907771027054 1101 9 -0.5751202391258019 1101 10-11 -0.5322847543861897 1101 12-13 -0.6062941312434162 1101 14-15 -0.7325800426197517 1101 16-17 -0.6728430557179053 1101 18-19 -0.6614514442344657 1101 20-21 -0.7407628572617 1101 22-23 -0.7682525704743526 1101 24-25 -0.8961265520009931 1101 26-27 -0.9198407028967566 1101 28-29 -0.8387417473077221 1101 30-31 -0.8982378665021642 1101 32-33 -0.9198740377936687 1101 34-35 -0.9144013684317116 1101 36-37 -0.9119232508920021 1101 38-39 -0.92995890456654 1101 40-41 -0.9073162719272219 1101 42-43 -1.05584484491974 1101 44-45 -1.0429340619747123 1101 46-47 -1.1499915772293505 1101 48-49 -1.211875258738246 1101 50-51 -1.235955431381491 1101 52-53 -1.0460147059658667 1101 54-55 -1.0320522486863055 1101 56-57 -0.9368962388942705 1101 58-59 -0.8567167657298498 1101 60-61 -0.859490027966892 1101 62-63 -0.9075699470295575 1101 64-65 -0.8166062297779675 1101 66-67 -0.9014880256968851 1101 68-69 -0.9324060079771854 1101 70-71 -1.0198517247067187 1101 72-73 -1.036462013280243 1101 74-75 -0.9572438845694293 1101 76-77 -0.9951489754472647 1101 78-79 -1.0183204364542249 1101 80-81 -1.0613324264460964 1101 82-83 -1.0687604454056512 1101 84-85 -1.0886167156128153 1101 86-87 -1.1981036921061552 1101 88-89 -1.3009201160520867 1101 90-91 -1.4079394257807536 1101 92-93 -1.4459799111046223 1101 94-95 -1.466707685270432 1101 96-97 -1.5646566757399896 1101 98-99 -1.6772377727615435 1101 100 -1.7475643635518345 1102 1 -0.43340704785788375 1102 2 -0.43611000037748937 1102 3 -0.476481292438951 1102 4 -0.324543975093853 1102 5 -0.37772592316654396 1102 6 -0.35217342492179426 1102 7 -0.3675164818968639 1102 8 -0.4365813173334345 1102 9 -0.46178405888690577 1102 10-11 -0.5667735037196167 1102 12-13 -0.5874725410443133 1102 14-15 -0.7381839930483736 1102 16-17 -0.7461908367322465 1102 18-19 -0.7380588821359098 1102 20-21 -0.7658375763756169 1102 22-23 -0.8056915938663352 1102 24-25 -0.766668148092748 1102 26-27 -0.8148827876874734 1102 28-29 -0.8545443829997907 1102 30-31 -0.8604947013110475 1102 32-33 -0.9283842741049355 1102 34-35 -0.9603254428274184 1102 36-37 -0.9211048320019728 1102 38-39 -1.0344882305439569 1102 40-41 -1.1435500935921183 1102 42-43 -1.1203161524314922 1102 44-45 -1.279131412558705 1102 46-47 -1.2831556455746451 1102 48-49 -1.1737983696490417 1102 50-51 -1.2231092090465978 1102 52-53 -1.098434752643989 1102 54-55 -1.0140655625759578 1102 56-57 -0.9530589454634324 1102 58-59 -0.9620818062644787 1102 60-61 -0.8500170901978308 1102 62-63 -0.9860872741294102 1102 64-65 -0.9472635259163056 1102 66-67 -1.0075828052831 1102 68-69 -0.9841415329901437 1102 70-71 -1.0552167052666874 1102 72-73 -1.0721589279537724 1102 74-75 -0.9828352300521743 1102 76-77 -1.0678424787779832 1102 78-79 -1.045699607830727 1102 80-81 -1.1484107319370018 1102 82-83 -1.1726835950028125 1102 84-85 -1.259791167355889 1102 86-87 -1.2306110486608688 1102 88-89 -1.2743152730005214 1102 90-91 -1.2960260026696702 1102 92-93 -1.333018065987794 1102 94-95 -1.3624561203436265 1102 96-97 -1.3904836865412946 1102 98-99 -1.4389047972500357 1102 100 -1.585417220246505 1103 1 -0.49320151176934957 1103 2 -0.4739385747230749 1103 3 -0.5690356937480416 1103 4 -0.4852555159784444 1103 5 -0.4389086429342157 1103 6 -0.4823340187287286 1103 7 -0.46492406621580074 1103 8 -0.43294278729811 1103 9 -0.6722285916520718 1103 10-11 -0.6470674699154699 1103 12-13 -0.6677794109782837 1103 14-15 -0.765406565717484 1103 16-17 -0.7936474836158851 1103 18-19 -0.6779479221423585 1103 20-21 -0.7764627614743773 1103 22-23 -0.8235504079297513 1103 24-25 -0.8959325904547626 1103 26-27 -0.8939271777620164 1103 28-29 -0.92116992786368 1103 30-31 -0.9178650855912593 1103 32-33 -0.9502194854689705 1103 34-35 -0.9093072224250918 1103 36-37 -0.8939109635666185 1103 38-39 -0.9325180910498858 1103 40-41 -0.9624598431866715 1103 42-43 -1.0430283520752113 1103 44-45 -1.0230963322172286 1103 46-47 -1.0539597530552882 1103 48-49 -1.1365567229456985 1103 50-51 -1.1647887693228611 1103 52-53 -1.0003580781054353 1103 54-55 -0.805274774508014 1103 56-57 -0.7419033296229074 1103 58-59 -0.7748146761976287 1103 60-61 -0.782601500294426 1103 62-63 -0.8211020435414369 1103 64-65 -0.763896221517328 1103 66-67 -0.8405449485109457 1103 68-69 -0.8084678950637638 1103 70-71 -0.8623277385907429 1103 72-73 -0.902753772049472 1103 74-75 -0.9014918678606492 1103 76-77 -0.916693134518809 1103 78-79 -0.8961481798508046 1103 80-81 -0.9746078524384707 1103 82-83 -0.9214623382313896 1103 84-85 -1.0267516016993596 1103 86-87 -1.1344363284005397 1103 88-89 -1.067808385807556 1103 90-91 -1.116776206151691 1103 92-93 -1.2154977674151475 1103 94-95 -1.1802838398418878 1103 96-97 -1.2671157055569893 1103 98-99 -1.2499710187478676 1103 100 -1.4177108809661085 1104 1 -0.5111059252436156 1104 2 -0.47022682268996263 1104 3 -0.5382047034124575 1104 4 -0.3923729176274975 1104 5 -0.35123498044691104 1104 6 -0.43178351883408084 1104 7 -0.47876694635295536 1104 8 -0.4464281037015425 1104 9 -0.5051017693718336 1104 10-11 -0.5802879969299326 1104 12-13 -0.4899222135051531 1104 14-15 -0.6925935889828665 1104 16-17 -0.7364440763903275 1104 18-19 -0.7319003944549038 1104 20-21 -0.6781522685994759 1104 22-23 -0.6885394551327195 1104 24-25 -0.762646554838085 1104 26-27 -0.7990544370256814 1104 28-29 -0.7679079710845684 1104 30-31 -0.7990886703223694 1104 32-33 -0.8771648642913803 1104 34-35 -0.9801287726734174 1104 36-37 -0.939819905636746 1104 38-39 -0.891260454297516 1104 40-41 -0.9015064138298285 1104 42-43 -0.9982025880303027 1104 44-45 -0.9949790945828809 1104 46-47 -1.0221296738476582 1104 48-49 -0.9684682647364653 1104 50-51 -1.0303681138204794 1104 52-53 -0.8458685014054126 1104 54-55 -0.6871103500566278 1104 56-57 -0.7696323602065007 1104 58-59 -0.7113404205724478 1104 60-61 -0.7077095058269549 1104 62-63 -0.7683390183320071 1104 64-65 -0.719244180759965 1104 66-67 -0.7101857096092274 1104 68-69 -0.8220597723862184 1104 70-71 -0.8272985554520815 1104 72-73 -0.6947543098153659 1104 74-75 -0.7864645991996966 1104 76-77 -0.785986993812088 1104 78-79 -0.7789482708274065 1104 80-81 -0.7605907626119155 1104 82-83 -0.7629078039251702 1104 84-85 -0.8165139905005994 1104 86-87 -0.7884774425876593 1104 88-89 -0.8842465116973024 1104 90-91 -0.9217956203954465 1104 92-93 -1.0207766284098625 1104 94-95 -0.9538839315189058 1104 96-97 -1.0086239365465062 1104 98-99 -0.9946602595193177 1104 100 -1.061474790843235 1105 1 -0.5669908814777713 1105 2 -0.5006830079832731 1105 3 -0.5224682267119327 1105 4 -0.3027655495878747 1105 5 -0.34841440230592013 1105 6 -0.30962441847020017 1105 7 -0.36940960632472297 1105 8 -0.3878122511603621 1105 9 -0.5173577362015038 1105 10-11 -0.5114776117080098 1105 12-13 -0.5548120692366609 1105 14-15 -0.7991581164364945 1105 16-17 -0.7240501641757504 1105 18-19 -0.7648978066662906 1105 20-21 -0.7664218784136665 1105 22-23 -0.7242571994991991 1105 24-25 -0.7908113844012519 1105 26-27 -0.7786747256030182 1105 28-29 -0.7437805385184006 1105 30-31 -0.8421717653697769 1105 32-33 -0.8593717942943755 1105 34-35 -0.779699460918593 1105 36-37 -0.7765445019272974 1105 38-39 -0.8622727617614387 1105 40-41 -0.8734221831991178 1105 42-43 -0.917178561921574 1105 44-45 -0.9899717306882394 1105 46-47 -1.0119428184563333 1105 48-49 -1.0313529171062825 1105 50-51 -1.0064980469390576 1105 52-53 -0.8772850349234247 1105 54-55 -0.7757139597794378 1105 56-57 -0.6662553197745922 1105 58-59 -0.6723294089946421 1105 60-61 -0.6641539549273574 1105 62-63 -0.7284989603486807 1105 64-65 -0.7245368082728092 1105 66-67 -0.7378785686660834 1105 68-69 -0.6980821944583226 1105 70-71 -0.6026492676473651 1105 72-73 -0.6294965366922014 1105 74-75 -0.6948118638508163 1105 76-77 -0.6738692333195146 1105 78-79 -0.7940472551661415 1105 80-81 -0.7748877034251329 1105 82-83 -0.8421284297082217 1105 84-85 -0.8540305401648709 1105 86-87 -0.8354349025379229 1105 88-89 -0.8751943760695262 1105 90-91 -0.9626675867567656 1105 92-93 -0.9947396056208433 1105 94-95 -0.9852815617602779 1105 96-97 -0.9127004599854587 1105 98-99 -0.8948477253118874 1105 100 -1.0108977032432698 1106 1 -0.5854214477719317 1106 2 -0.5194469059764195 1106 3 -0.554573260043103 1106 4 -0.46264322723578744 1106 5 -0.49893276374184836 1106 6 -0.459589524332344 1106 7 -0.47181034252124476 1106 8 -0.46639429238413044 1106 9 -0.5188955272857569 1106 10-11 -0.5398516189298874 1106 12-13 -0.6542380670478991 1106 14-15 -0.913588493463287 1106 16-17 -0.8605721527108443 1106 18-19 -0.9559515228256856 1106 20-21 -0.8409734515941949 1106 22-23 -0.8353193879122358 1106 24-25 -0.8578610556523927 1106 26-27 -0.8897989587399806 1106 28-29 -0.8899113855066219 1106 30-31 -0.9511647143974713 1106 32-33 -0.9624165798659092 1106 34-35 -0.9233394633340879 1106 36-37 -1.0124433765694718 1106 38-39 -1.0108689937849178 1106 40-41 -1.0382196991387502 1106 42-43 -0.9712261205626831 1106 44-45 -1.0233330617996614 1106 46-47 -1.011251902519362 1106 48-49 -0.9468505797068616 1106 50-51 -1.0451222227905035 1106 52-53 -1.0072674932958563 1106 54-55 -0.8203911390983336 1106 56-57 -0.8443209570262482 1106 58-59 -0.7638148453637328 1106 60-61 -0.7671075652110986 1106 62-63 -0.8360896836061684 1106 64-65 -0.816739495659256 1106 66-67 -0.8496983143403796 1106 68-69 -0.8585676927658241 1106 70-71 -0.825300683790509 1106 72-73 -0.7306274417164076 1106 74-75 -0.7259119302519892 1106 76-77 -0.8280711664382423 1106 78-79 -0.8199055023652271 1106 80-81 -0.7542701559839635 1106 82-83 -0.7507772078155206 1106 84-85 -0.8491617147562351 1106 86-87 -0.9002900004341292 1106 88-89 -0.836636022284349 1106 90-91 -0.8980100363597643 1106 92-93 -0.9749461379415365 1106 94-95 -1.0451669591952282 1106 96-97 -1.0517563698051404 1106 98-99 -1.0393533269854842 1106 100 -1.2952753833427018 1107 1 -0.4786685313535486 1107 2 -0.5068124793785671 1107 3 -0.5647879656262589 1107 4 -0.43566117810192395 1107 5 -0.45802807508673027 1107 6 -0.460344463179986 1107 7 -0.3967548883614924 1107 8 -0.3839468649263438 1107 9 -0.5902975604583887 1107 10-11 -0.5609847750244867 1107 12-13 -0.5883699006002558 1107 14-15 -0.790851488213022 1107 16-17 -0.7144056317753851 1107 18-19 -0.7536307346725195 1107 20-21 -0.7375727938478605 1107 22-23 -0.7817547185653098 1107 24-25 -0.8017117853531346 1107 26-27 -0.8820299507990157 1107 28-29 -0.8640932387024947 1107 30-31 -0.8766425465608307 1107 32-33 -0.8957804801331974 1107 34-35 -0.8825967703659003 1107 36-37 -0.967672487607139 1107 38-39 -0.8806138920352851 1107 40-41 -0.9308429517451486 1107 42-43 -0.9127712378005626 1107 44-45 -0.9186313804027364 1107 46-47 -0.8495794537566965 1107 48-49 -0.8401254708946766 1107 50-51 -0.9371646264934554 1107 52-53 -0.8830834576223623 1107 54-55 -0.7247912956586475 1107 56-57 -0.7304835859990959 1107 58-59 -0.7279580608675218 1107 60-61 -0.7039645931601086 1107 62-63 -0.6377163630453495 1107 64-65 -0.5781181280668548 1107 66-67 -0.7309726282371471 1107 68-69 -0.6880869076441201 1107 70-71 -0.8065902738058455 1107 72-73 -0.7455382096127039 1107 74-75 -0.7075520455230588 1107 76-77 -0.7245037059204513 1107 78-79 -0.631143166559621 1107 80-81 -0.6834069449362481 1107 82-83 -0.7110276523881716 1107 84-85 -0.7489146636790807 1107 86-87 -0.7679416919975743 1107 88-89 -0.7672858793663551 1107 90-91 -0.8170515681249455 1107 92-93 -0.7953840230487117 1107 94-95 -0.855412692212667 1107 96-97 -0.8733355363882183 1107 98-99 -0.8691559700173137 1107 100 -0.8798550252943897 1108 1 -0.49543659535888196 1108 2 -0.44779120291416774 1108 3 -0.5109313170783878 1108 4 -0.36039502937965295 1108 5 -0.3704000937426102 1108 6 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1.0908812287182421 2310 88-89 1.1432465730316217 2310 90-91 1.264783419261704 2310 92-93 1.2878294254124896 2310 94-95 1.315109336370142 2310 96-97 1.3267143731507431 2310 98-99 1.390822188560648 2310 100 1.6319684365080178 2311 1 0.7388388917487987 2311 2 0.7078990234195643 2311 3 0.7224905347241162 2311 4 0.6651808854015684 2311 5 0.6339477353909899 2311 6 0.6620081094423114 2311 7 0.711188927121114 2311 8 0.6347765321686154 2311 9 0.8261488536390402 2311 10-11 0.8535568535058715 2311 12-13 0.9596644465904447 2311 14-15 1.1516260166032382 2311 16-17 1.1683277624950534 2311 18-19 1.0876066381766663 2311 20-21 1.1177398819823736 2311 22-23 1.154177570786338 2311 24-25 1.1908618016027859 2311 26-27 1.2074893720268634 2311 28-29 1.2626201211646162 2311 30-31 1.2081775789383329 2311 32-33 1.2429987355406666 2311 34-35 1.251878233546698 2311 36-37 1.263701391111418 2311 38-39 1.298083964286434 2311 40-41 1.3501030632359345 2311 42-43 1.3991609002106529 2311 44-45 1.3787171206678934 2311 46-47 1.3525900044362658 2311 48-49 1.4249525333126059 2311 50-51 1.418551838120372 2311 52-53 1.3308456724522202 2311 54-55 1.2404903610529416 2311 56-57 1.1371457285168276 2311 58-59 1.1023540019266491 2311 60-61 1.0740998485835362 2311 62-63 1.0774530336516293 2311 64-65 1.1193146115499033 2311 66-67 1.1450304197518406 2311 68-69 1.1123769910720753 2311 70-71 1.1104750586675536 2311 72-73 1.0989440982879657 2311 74-75 1.09631831388252 2311 76-77 1.1407382051977564 2311 78-79 1.1358116898290618 2311 80-81 1.1560407625204725 2311 82-83 1.1389402905335224 2311 84-85 1.1243935719273956 2311 86-87 1.0710109293557757 2311 88-89 1.1971537614267085 2311 90-91 1.2169558760666561 2311 92-93 1.2760968882231722 2311 94-95 1.2932455971111558 2311 96-97 1.3082860261120288 2311 98-99 1.3631467519903815 2311 100 1.5187131006491974 2312 1 0.6427838277497031 2312 2 0.6410509298498468 2312 3 0.6395435595095407 2312 4 0.3573444992288657 2312 5 0.4612603065507628 2312 6 0.5516714829256628 2312 7 0.3890137125793274 2312 8 0.5203178953722585 2312 9 0.7299721230914145 2312 10-11 0.6912604044181165 2312 12-13 0.8254018598705031 2312 14-15 1.0093156558424 2312 16-17 1.0081579735822999 2312 18-19 0.989615790253005 2312 20-21 1.0136272133328958 2312 22-23 1.074772310755442 2312 24-25 1.0570120239459158 2312 26-27 1.0893936903474426 2312 28-29 1.100593346275076 2312 30-31 1.1237155630825626 2312 32-33 1.1617382541103822 2312 34-35 1.168133371709068 2312 36-37 1.1488182607732327 2312 38-39 1.159534951193315 2312 40-41 1.2273536977290647 2312 42-43 1.2791356637774811 2312 44-45 1.3285003689096229 2312 46-47 1.3622684053158451 2312 48-49 1.3454517862585647 2312 50-51 1.3972211758328044 2312 52-53 1.2710441019443195 2312 54-55 1.140845891508505 2312 56-57 1.1463654243130392 2312 58-59 1.1108612846777177 2312 60-61 1.0944499033215749 2312 62-63 1.1035531360438924 2312 64-65 1.1132590368863688 2312 66-67 1.074634594427316 2312 68-69 1.0375101560746458 2312 70-71 1.0521721118530607 2312 72-73 1.1008024066348128 2312 74-75 1.1352121891789437 2312 76-77 1.0991331694085176 2312 78-79 1.107133684427417 2312 80-81 1.1225047990086985 2312 82-83 1.1359712933930304 2312 84-85 1.1430610702273363 2312 86-87 1.1816109583798884 2312 88-89 1.1613197823926917 2312 90-91 1.1575502809786613 2312 92-93 1.2391579605870433 2312 94-95 1.2429920315911858 2312 96-97 1.2439368547682044 2312 98-99 0.8770768744703368 2312 100 1.2058869403088117 2313 1 0.6629846872227461 2313 2 0.652632064681363 2313 3 0.6583507190508868 2313 4 0.5648568224160826 2313 5 0.42813134953423315 2313 6 0.5347245703505834 2313 7 0.6149997387777546 2313 8 0.5539528422041329 2313 9 0.7488931633477236 2313 10-11 0.7707248259158845 2313 12-13 0.8853166692401615 2313 14-15 1.0212288815596509 2313 16-17 1.0505416716334537 2313 18-19 1.0273197962780145 2313 20-21 1.035971325850106 2313 22-23 1.0773092943448574 2313 24-25 1.0645394847442944 2313 26-27 1.0996457808767062 2313 28-29 1.134850837164656 2313 30-31 1.1475042468167302 2313 32-33 1.1680282182908854 2313 34-35 1.1853210667827128 2313 36-37 1.244393935997742 2313 38-39 1.29822080603018 2313 40-41 1.3590879151087947 2313 42-43 1.3760887625979095 2313 44-45 1.3698002734135244 2313 46-47 1.3697290155517763 2313 48-49 1.3268599653215318 2313 50-51 1.3247294623237096 2313 52-53 1.2171297195138848 2313 54-55 1.150913483892289 2313 56-57 1.0829465735849055 2313 58-59 1.0544070099590854 2313 60-61 1.0479480241371206 2313 62-63 1.0640597810384804 2313 64-65 1.052308425436614 2313 66-67 1.0427308762355878 2313 68-69 1.0495557915370384 2313 70-71 1.0730642596948385 2313 72-73 1.0852101019048348 2313 74-75 1.0107801920399453 2313 76-77 0.7975561430624722 2313 78-79 0.9428014073849553 2313 80-81 1.01032026802174 2313 82-83 1.033104995590623 2313 84-85 1.0417234731128993 2313 86-87 1.0899119927791894 2313 88-89 1.07969477530661 2313 90-91 1.118915598611455 2313 92-93 1.1858633620448273 2313 94-95 1.2339872241103258 2313 96-97 1.3052849883861555 2313 98-99 1.3647810331504573 2313 100 1.4348004047456584 2314 1 0.591634059507868 2314 2 0.5616380438337814 2314 3 0.5934048429553727 2314 4 0.43458656190175304 2314 5 0.45831817107267625 2314 6 0.4980191896627133 2314 7 0.4987702733545518 2314 8 0.5384817745297354 2314 9 0.6701083684982905 2314 10-11 0.6727227315057789 2314 12-13 0.7345350752520332 2314 14-15 0.8881777599830301 2314 16-17 0.882594005205739 2314 18-19 0.8283700460338679 2314 20-21 0.8801930335594434 2314 22-23 0.90471760112532 2314 24-25 0.9407685303438171 2314 26-27 0.9818522603647963 2314 28-29 0.9513262015578832 2314 30-31 0.9934516417323138 2314 32-33 0.9832470033211465 2314 34-35 1.0808007506515551 2314 36-37 1.1414016368196158 2314 38-39 1.0742792619657635 2314 40-41 1.1121194069855989 2314 42-43 1.2320510435980552 2314 44-45 1.2912039087727294 2314 46-47 1.2882486623191909 2314 48-49 1.24269574206793 2314 50-51 1.2626049740083474 2314 52-53 1.232771427858843 2314 54-55 1.134205957002969 2314 56-57 1.1004687271313003 2314 58-59 1.0537634830321068 2314 60-61 1.0110639280390927 2314 62-63 1.0489727225546872 2314 64-65 1.0577206157823227 2314 66-67 1.033343253814806 2314 68-69 0.9908624476257231 2314 70-71 1.055716627245367 2314 72-73 1.1475711549795733 2314 74-75 1.1672453472628845 2314 76-77 1.11157220386864 2314 78-79 1.0703056144498362 2314 80-81 1.0811367662292461 2314 82-83 1.1432261951563625 2314 84-85 1.221559490767465 2314 86-87 1.1979921273110925 2314 88-89 1.1851054511481856 2314 90-91 1.2321711350853022 2314 92-93 1.2800157125277636 2314 94-95 1.3111386811927694 2314 96-97 1.3178816174766368 2314 98-99 1.4308953609480035 2314 100 1.4867417518460684 2315 1 0.4294542851459653 2315 2 0.46840658382923905 2315 3 0.49766603956571487 2315 4 0.21572530450532668 2315 5 0.3164605168740735 2315 6 0.4073766177854736 2315 7 0.39353861136971346 2315 8 0.4094155704977709 2315 9 0.5701058226250524 2315 10-11 0.4932167729912251 2315 12-13 0.5372496826701223 2315 14-15 0.7513661570985519 2315 16-17 0.7443262703474858 2315 18-19 0.6474471497056342 2315 20-21 0.7439850944668649 2315 22-23 0.5363318172353999 2315 24-25 0.7154328523495508 2315 26-27 0.8157490649482853 2315 28-29 0.8237105462422392 2315 30-31 0.8829349158635367 2315 32-33 0.8866856601599054 2315 34-35 0.8911428096486134 2315 36-37 0.9717411192239993 2315 38-39 1.0020488638937124 2315 40-41 1.0593380635133158 2315 42-43 1.0650074110554968 2315 44-45 1.0859118408574346 2315 46-47 1.1048461200170863 2315 48-49 1.1169387201329073 2315 50-51 1.04033641663721 2315 52-53 1.0356069661756777 2315 54-55 0.8847791029554273 2315 56-57 0.8413530283284558 2315 58-59 0.8628376587576483 2315 60-61 0.8332539281331677 2315 62-63 0.8715708457123625 2315 64-65 0.8897791616509565 2315 66-67 0.8499271373620303 2315 68-69 0.8142560044928686 2315 70-71 0.788565134923978 2315 72-73 0.5663276591001107 2315 74-75 0.7844339045034658 2315 76-77 0.8525604020374118 2315 78-79 0.8941238867158177 2315 80-81 0.5765432928421639 2315 82-83 0.5385020374496925 2315 84-85 0.8257979511554261 2315 86-87 0.9326833557026184 2315 88-89 1.0099324613656613 2315 90-91 1.0349602217753606 2315 92-93 1.0556149076017434 2315 94-95 1.0570371046905258 2315 96-97 0.9027025798052009 2315 98-99 0.36786676633723303 2315 100 0.9041038316593877 2316 1 0.3392621673467211 2316 2 0.29239379135850996 2316 3 0.34155163768345176 2316 4 0.2246466902086297 2316 5 0.16423575957113457 2316 6 0.21531297439948105 2316 7 0.16161914563312507 2316 8 0.24446237716993835 2316 9 0.27123870587108456 2316 10-11 0.30288943714503347 2316 12-13 0.17112291576657412 2316 14-15 0.12111539915419911 2316 16-17 0.30374643454085515 2316 18-19 0.36945078637292994 2316 20-21 0.33195379958377913 2316 22-23 0.29692827593838444 2316 24-25 0.38628889533541155 2316 26-27 0.44939199649885353 2316 28-29 0.3987624370412419 2316 30-31 0.2896012093293834 2316 32-33 0.4988129305753617 2316 34-35 0.49967720279807537 2316 36-37 0.5046548806850026 2316 38-39 0.6753448425149671 2316 40-41 0.7445349780021004 2316 42-43 0.6552550258574001 2316 44-45 0.5742160610869789 2316 46-47 0.4353795969075094 2316 48-49 0.37258767844384977 2316 50-51 0.42420681091838475 2316 52-53 0.5698871968740775 2316 54-55 0.2828708911405826 2316 56-57 0.15595005720622623 2316 58-59 0.18261103634727505 2316 60-61 0.26158609065328164 2316 62-63 0.4031931182167199 2316 64-65 0.3544876479430954 2316 66-67 0.2958685270389836 2316 68-69 0.2601276199941083 2316 70-71 0.10065070702742673 2316 72-73 0.002926954129321757 2316 74-75 0.22103623739354106 2316 76-77 0.38921339531554366 2316 78-79 0.3732846056876582 2316 80-81 0.6032964425376584 2316 82-83 0.5537782840902032 2316 84-85 0.6078169137921101 2316 86-87 0.6781367930371509 2316 88-89 0.6334075334794953 2316 90-91 0.43264011313156203 2316 92-93 0.4709626962519984 2316 94-95 0.6331975325610948 2316 96-97 0.6002360153482513 2316 98-99 0.43062814073566713 2316 100 0.3087460942853646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 43.0 1 590.0 2 229.0 3 204.0 4 149.0 5 117.0 6 174.0 7 671.0 8 2268.0 9 4583.0 10 6842.0 11 9813.0 12 13483.0 13 16892.0 14 20086.0 15 24219.0 16 29480.0 17 37469.0 18 46847.0 19 57778.0 20 72464.0 21 90363.0 22 99346.0 23 97970.0 24 94107.0 25 95426.0 26 105406.0 27 119749.0 28 141861.0 29 171475.0 30 209005.0 31 262224.0 32 334367.0 33 443459.0 34 644610.0 35 899679.0 36 1365688.0 37 1890561.0 38 1615164.0 39 496519.0 40 442.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.269536115829915 20.97776887609026 13.007989031028663 22.74470597705116 2 15.472795460487172 19.86795089051069 41.78829656585661 22.87095708314553 3 19.064302914599786 27.03056403900138 28.613549645883612 25.291583400515222 4 12.278393643496887 16.521906098010724 39.766221371540084 31.433478886952305 5 12.823966303124193 38.563412158467926 34.58468667794195 14.02793486046593 6 29.244219952315852 38.38709270667946 18.223634229608933 14.145053111395756 7 27.03830869903677 32.2806346855155 23.128153545842324 17.552903069605406 8 26.162196660650807 35.364372878022046 19.89672445199486 18.576706009332288 9 26.395477493108515 15.491252436066038 21.925293029234265 36.18797704159118 10-11 25.744919838234992 26.420633990067664 27.05554652004769 20.778899651649652 12-13 25.847901550074965 24.105194694057598 27.880830156291392 22.16607359957604 14-15 23.16684664645424 25.220612012509807 25.90731005050866 25.70523129052729 16-17 21.523286091883882 28.011644372078525 27.112203392483224 23.352866143554365 18-19 20.928115628430223 27.854035913299686 29.69098175974118 21.52686669852891 20-21 22.546362201654148 26.983319229431128 29.122523693715348 21.347794875199373 22-23 22.88909989687361 26.721536796434474 28.798556041476196 21.590807265215712 24-25 22.21302994077395 26.71186180728739 29.626218293894823 21.448889958043836 26-27 22.05807726555527 27.004871757514437 28.679130707618604 22.257920269311686 28-29 21.317127064627 27.365449654156627 29.246566802460617 22.070856478755758 30-31 22.304080903596315 27.048742005084097 29.123384013623987 21.5237930776956 32-33 22.48307372400657 27.285889600841635 28.286273808463964 21.944762866687835 34-35 22.4650885076463 27.140319213897513 29.066140886781373 21.328451391674815 36-37 22.12608439339099 26.78446423424158 28.666075325790725 22.423376046576706 38-39 22.268131228631702 26.97371451441063 29.17218564785648 21.58596860910119 40-41 22.056483275565668 26.050992415006952 29.67471352088959 22.21781078853779 42-43 22.005886593179767 26.324878737997963 28.960692679294976 22.7085419895273 44-45 22.100742289487645 27.188803143567085 28.821172848910205 21.88928171803506 46-47 22.108715932198976 26.985156855846405 28.186801504777176 22.719325707177447 48-49 22.770621788512035 26.91759983920956 28.13217490813964 22.179603464138765 50-51 22.491526305090837 26.73056407783666 28.122492886718533 22.655416730353974 52-53 21.991859299085263 27.332091017455234 27.865045684867994 22.81100399859151 54-55 21.92808926412339 27.240763057366934 27.890893918245713 22.940253760263964 56-57 22.824988934242242 26.816058161181523 27.41599400972946 22.942958894846775 58-59 21.918150049486954 27.180162981236982 28.234030213308735 22.66765675596733 60-61 22.02965007009481 27.812578177469838 27.580084323863595 22.577687428571757 62-63 22.34482607929708 27.135732869633035 27.31972619608005 23.199714854989832 64-65 21.650702718868807 27.219609054901827 27.731567847342774 23.39812037888659 66-67 22.2066669723788 26.866444820508896 27.569236051108977 23.35765215600333 68-69 21.78201334232249 26.981084420652664 27.840328560233395 23.396573676791448 70-71 21.863060534503518 26.624728650451562 27.91875927295368 23.593451542091245 72-73 22.449130548211517 26.830899425892106 27.98282940282209 22.737140623074282 74-75 22.65757683067737 27.106564920844306 27.520629980617223 22.7152282678611 76-77 22.365475070826783 27.02798107602296 27.25902914432592 23.347514708824338 78-79 22.226363813789934 27.08314469454592 27.309831619005042 23.380659872659102 80-81 21.89403395261643 27.49537213186152 27.77608534564098 22.83450856988107 82-83 22.821978107397857 26.577491896564897 28.71613699013617 21.88439300590107 84-85 22.906773800870308 27.020450138643447 27.49261880327535 22.580157257210896 86-87 21.787924060470502 27.709024269720206 27.86077140208857 22.64228026772072 88-89 22.71236116300442 26.56502837637592 27.774274067608978 22.948336393010678 90-91 22.53007218711485 26.69942666071279 27.799518332392665 22.970982819779696 92-93 22.84888636856978 26.886178127772304 27.655335892483144 22.609599611174776 94-95 21.780079467833385 26.878552876572748 28.299414627228632 23.041953028365235 96-97 22.205973011112583 26.796764703456976 27.798215787247493 23.199046498182955 98-99 21.689548551368986 27.30031788872429 27.607239318088478 23.40289424181824 100 22.4398304208719 26.810476541049695 26.786797821978052 23.962895216100353 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1515.0 1 1080.0 2 1242.0 3 1750.5 4 1332.5 5 1106.5 6 1580.0 7 2832.0 8 4476.5 9 5391.0 10 5127.5 11 4881.0 12 5426.5 13 6481.0 14 7687.5 15 8947.0 16 10267.0 17 11475.5 18 12752.5 19 13874.0 20 14846.0 21 15931.0 22 17585.0 23 19991.0 24 22634.5 25 26023.5 26 30994.5 27 37041.5 28 45857.0 29 63164.0 30 88693.5 31 112895.5 32 145402.5 33 181173.5 34 208723.0 35 236702.5 36 264617.0 37 306773.0 38 324615.5 39 310293.5 40 290460.0 41 266215.0 42 258378.0 43 271404.5 44 294709.0 45 338874.5 46 381767.5 47 411017.5 48 432677.5 49 427144.0 50 407876.5 51 414301.5 52 411635.0 53 386278.0 54 361020.0 55 309149.0 56 266172.0 57 217139.5 58 169706.0 59 158693.0 60 131246.5 61 91441.5 62 68316.0 63 57176.5 64 42400.5 65 21888.0 66 12523.5 67 8589.0 68 6417.5 69 4963.0 70 3960.5 71 3165.0 72 2617.5 73 2343.5 74 1907.5 75 1527.0 76 1203.5 77 819.0 78 558.5 79 417.5 80 300.5 81 226.0 82 192.0 83 144.0 84 111.0 85 80.0 86 51.5 87 41.0 88 34.5 89 25.5 90 19.0 91 17.5 92 14.0 93 8.5 94 7.5 95 7.5 96 5.0 97 4.5 98 4.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05801410696398231 2 0.13426001872330737 3 0.017297109733830352 4 0.017003048366163533 5 0.019838640125807855 6 0.05284702864640822 7 0.058287163948244354 8 0.049969428119954355 9 0.04111608051484264 10-11 0.04009736791971116 12-13 0.041247357911122474 14-15 0.018604632600777455 16-17 0.017711946306074616 18-19 0.01755966452639001 20-21 0.02098337902136797 22-23 0.025572836795310812 24-25 0.024806176801036608 26-27 0.019418552457712398 28-29 0.0184943595879024 30-31 0.016309903713806032 32-33 0.023414636400470413 34-35 0.02361942913866695 36-37 0.023251852429083425 38-39 0.02126168710148121 40-41 0.02485868775954854 42-43 0.03227848619728451 44-45 0.04171470544187867 46-47 0.047601183891066226 48-49 0.040359922712270825 50-51 0.02790432335324059 52-53 0.04038617819152679 54-55 0.03683643739612019 56-57 0.039078655324579686 58-59 0.043710121865332074 60-61 0.023267605716637003 62-63 0.025782880629358542 64-65 0.025242017756685647 66-67 0.03217346428026065 68-69 0.038742585190103325 70-71 0.04081676805132463 72-73 0.044875865144296964 74-75 0.026728077882573312 76-77 0.02039000519018314 78-79 0.019576085333248193 80-81 0.02436508474953638 82-83 0.020369000806778365 84-85 0.01236633072955995 86-87 0.014477271261739613 88-89 0.01843134643768808 90-91 0.02078383737902263 92-93 0.019570834237397004 94-95 0.023598424755262176 96-97 0.025052978306042688 98-99 0.02741597143907962 100 0.028628974580705246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9521822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.096946370113116 #Duplication Level Percentage of deduplicated Percentage of total 1 75.44803486713789 47.60540609742224 2 14.745201045128278 18.607543191219893 3 5.02359461372914 9.509204397829699 4 2.0727073443724064 5.231260165952213 5 1.0177102015366688 3.2107203003338096 6 0.5403642427472121 2.045720018696857 7 0.3151979069530217 1.39216178016907 8 0.20334697700278187 1.0264458641975323 9 0.1233134196241486 0.7002630202266141 >10 0.46670849058920943 4.753206161160342 >50 0.022823632780728548 0.993781609520019 >100 0.018605038881995318 2.2788943997329856 >500 0.00153636326225513 0.6567932552170924 >1k 7.680761290699451E-4 0.8137396341406864 >5k 2.194503131106437E-5 0.09257866604463222 >10k+ 6.583509393319312E-5 1.0822814381364858 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34366 0.36091832004421004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27171 0.2853550507455401 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16625 0.17459893705217341 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.050219170238637E-5 0.0 7 0.0 0.0 0.0 1.050219170238637E-5 0.0 8 0.0 0.0 0.0 1.050219170238637E-5 0.0 9 0.0 0.0 0.0 1.050219170238637E-5 0.0 10-11 0.0 0.0 0.0 2.100438340477274E-5 0.0 12-13 0.0 0.0 0.0 4.200876680954548E-5 0.0 14-15 0.0 0.0 0.0 4.200876680954548E-5 0.0 16-17 0.0 0.0 0.0 5.776205436312504E-5 0.0 18-19 0.0 0.0 0.0 7.876643776789778E-5 0.0 20-21 0.0 0.0 0.0 1.5753287553579555E-4 0.0 22-23 0.0 0.0 0.0 3.8332999713710256E-4 0.0 24-25 0.0 0.0 0.0 9.504483490659667E-4 0.0 26-27 0.0 0.0 0.0 0.0021004383404772745 0.0 28-29 1.050219170238637E-5 0.0 0.0 0.0053141090014075035 0.0 30-31 1.050219170238637E-5 0.0 0.0 0.017969250002783082 0.0 32-33 1.050219170238637E-5 0.0 0.0 0.047716707999792475 0.0 34-35 1.050219170238637E-5 0.0 0.0 0.0913113057563983 0.0 36-37 1.050219170238637E-5 0.0 0.0 0.16438555562160267 0.0 38-39 1.050219170238637E-5 0.0 0.0 0.2694494814122759 0.0 40-41 1.050219170238637E-5 0.0 0.0 0.3801688374346842 0.0 42-43 1.050219170238637E-5 0.0 0.0 0.4871914219778526 0.0 44-45 1.050219170238637E-5 0.0 0.0 0.6146565226697159 0.0 46-47 1.050219170238637E-5 0.0 0.0 0.7653892290782164 0.0 48-49 1.050219170238637E-5 0.0 0.0 0.9301476125052537 0.0 50-51 1.050219170238637E-5 0.0 0.0 1.1015748876633065 0.0 52-53 1.050219170238637E-5 0.0 0.0 1.267367736973029 0.0 54-55 1.050219170238637E-5 0.0 0.0 1.445248608932198 0.0 56-57 1.050219170238637E-5 0.0 0.0 1.6514538919127033 0.0 58-59 1.050219170238637E-5 0.0 0.0 1.8915602497085118 0.0 60-61 1.050219170238637E-5 0.0 0.0 2.134780507344078 0.0 62-63 1.050219170238637E-5 0.0 0.0 2.3810726560525914 0.0 64-65 1.050219170238637E-5 0.0 0.0 2.6275275887324927 0.0 66-67 1.050219170238637E-5 0.0 0.0 2.9059721973378627 0.0 68-69 1.050219170238637E-5 0.0 0.0 3.208298789874459 0.0 70-71 1.050219170238637E-5 0.0 0.0 3.5370488967342597 0.0 72-73 1.050219170238637E-5 5.251095851193185E-6 0.0 3.866276853316519 0.0 74-75 2.100438340477274E-5 1.050219170238637E-5 0.0 4.184540521761486 0.0 76-77 2.100438340477274E-5 1.050219170238637E-5 0.0 4.538606161719889 0.0 78-79 2.100438340477274E-5 1.050219170238637E-5 0.0 4.924777001712487 0.0 80-81 2.100438340477274E-5 1.050219170238637E-5 0.0 5.336583691650611 0.0 82-83 2.100438340477274E-5 1.050219170238637E-5 0.0 5.7549910090736835 0.0 84-85 2.100438340477274E-5 1.050219170238637E-5 0.0 6.158327681403833 0.0 86-87 2.100438340477274E-5 1.050219170238637E-5 0.0 6.582500702071515 0.0 88 2.100438340477274E-5 1.050219170238637E-5 0.0 6.921679485291786 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19775 0.0 46.59319 1 GTATCAA 34895 0.0 39.808834 1 GTACTAG 9495 0.0 38.26032 1 GCATCGT 9220 0.0 36.80892 8 CAACGCA 39275 0.0 33.668808 5 ATCAACG 39785 0.0 33.2254 3 GGGCACC 9665 0.0 33.067158 94 TACCTGG 4875 0.0 31.950476 2 TAGGCAT 12330 0.0 31.758219 5 TATCAAC 43720 0.0 31.633219 2 GTGGTAT 6175 0.0 30.787231 1 AACGCAG 44605 0.0 30.43602 6 CTGTGCG 12205 0.0 29.770353 9 CTAGGCA 13540 0.0 29.371332 4 TCAACGC 46910 0.0 28.64974 4 ACCTGGG 5330 0.0 28.39899 3 TAGTACT 12980 0.0 28.139622 4 ACGCAGA 46880 0.0 28.09681 7 CGCAGAG 47015 0.0 27.994225 8 CATCGTC 12360 0.0 27.875828 9 >>END_MODULE