FastQCFastQC Report
Mon 6 Jun 2016
ERR1211189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9521822
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313790.3295482734291819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266340.27971537380135864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162350.17050308228824274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196950.047.4602131
GTATCAA343800.039.1137471
GGGCACC94500.035.46810594
CAACGCA397850.032.0654145
ATCAACG400250.031.9306623
GTGGTAT59300.030.684961
TATCAAC444050.030.2810172
TAGGCAT118650.030.1372285
TACCTGG47850.029.0732922
AACGCAG454200.029.059696
CTGTGCG123450.028.7751189
CTAGGCA132950.027.6470324
TCAACGC472050.027.3029484
TAGTACT133150.027.1465874
ACTGTGC141750.026.7839538
TACCCCG6350.026.6388325
GCATCGT117450.026.4844728
ACGCAGA482250.026.453697
CGCAGAG485250.026.2996888
ACCTGGG50850.026.2517033
Produced by FastQC (version 0.11.5)