##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211189_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9521822 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.180833143068625 33.0 31.0 34.0 30.0 34.0 2 32.32279840980014 34.0 31.0 34.0 31.0 34.0 3 32.399677078609535 34.0 31.0 34.0 31.0 34.0 4 36.008446807764315 37.0 35.0 37.0 35.0 37.0 5 35.9012925257372 37.0 35.0 37.0 35.0 37.0 6 35.92884124487939 37.0 35.0 37.0 35.0 37.0 7 35.86931188169659 37.0 35.0 37.0 35.0 37.0 8 35.86208857926561 37.0 35.0 37.0 35.0 37.0 9 37.46625183709588 39.0 37.0 39.0 35.0 39.0 10-11 37.44842006078248 39.0 37.0 39.0 35.0 39.0 12-13 37.338503282249974 39.0 37.0 39.0 34.0 39.0 14-15 38.55127495557048 40.0 38.0 41.0 34.0 41.0 16-17 38.51443111412921 40.0 38.0 41.0 34.0 41.0 18-19 38.5481788044347 40.0 38.0 41.0 34.0 41.0 20-21 38.515135706170526 40.0 38.0 41.0 34.0 41.0 22-23 38.375701625172155 40.0 38.0 41.0 34.0 41.0 24-25 38.30378613462844 40.0 38.0 41.0 34.0 41.0 26-27 38.16067854450546 40.0 38.0 41.0 33.5 41.0 28-29 38.14547572932996 40.0 37.5 41.0 33.5 41.0 30-31 38.15094758125073 40.0 37.5 41.0 34.0 41.0 32-33 38.01395489224646 40.0 37.0 41.0 33.0 41.0 34-35 37.86930663060074 40.0 37.0 41.0 33.0 41.0 36-37 37.729429829711165 40.0 37.0 41.0 33.0 41.0 38-39 37.62897279533266 40.0 36.5 41.0 33.0 41.0 40-41 37.51891491985462 40.0 36.0 41.0 32.5 41.0 42-43 37.364277130994466 40.0 36.0 41.0 32.0 41.0 44-45 37.187049915446856 39.5 36.0 41.0 31.5 41.0 46-47 36.917373691715724 39.0 35.5 41.0 31.0 41.0 48-49 36.641856516536436 39.0 35.0 41.0 30.5 41.0 50-51 36.42154017371885 39.0 35.0 40.5 30.0 41.0 52-53 36.198815730854875 38.5 35.0 40.0 29.5 41.0 54-55 35.94771819931101 38.0 35.0 40.0 29.0 41.0 56-57 35.6668185983733 38.0 34.5 40.0 28.5 41.0 58-59 35.368924980954276 37.5 34.0 40.0 28.0 41.0 60-61 35.10563587515078 37.0 34.0 40.0 28.0 41.0 62-63 34.792517492975605 36.5 34.0 39.5 27.5 41.0 64-65 34.41932967240933 36.0 33.5 39.0 26.0 41.0 66-67 34.12634026344958 35.0 33.0 39.0 26.0 40.0 68-69 33.81805761544376 35.0 33.0 38.5 26.0 40.0 70-71 33.539472277469585 35.0 33.0 38.0 26.0 40.0 72-73 33.23262590920099 35.0 32.5 37.0 25.5 40.0 74-75 32.920046762058774 35.0 32.0 36.5 25.0 39.0 76-77 31.945783433044642 34.0 30.5 35.5 24.5 38.5 78-79 32.45809557246502 35.0 32.0 36.0 25.0 38.5 80-81 32.406250767972764 35.0 32.0 35.5 25.0 37.5 82-83 32.174715458869116 35.0 32.0 35.0 25.0 37.0 84-85 31.94053837595368 34.0 32.0 35.0 24.5 36.5 86-87 31.71977553245587 34.0 31.0 35.0 24.0 36.0 88-89 31.51650765998356 34.0 31.0 35.0 24.0 36.0 90-91 31.30976203923997 34.0 31.0 35.0 23.5 35.5 92-93 31.12612717397994 34.0 31.0 35.0 23.0 35.0 94-95 30.954125691490557 34.0 31.0 35.0 21.5 35.0 96-97 30.796611877432703 34.0 30.0 35.0 20.0 35.0 98-99 30.63444160161784 34.0 30.0 35.0 20.0 35.0 100 28.788571136910562 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.32430487724754187 1101 2 -0.3126397745481455 1101 3 -0.3795645790577744 1101 4 -0.18458926627715755 1101 5 -0.24149136732393117 1101 6 -0.29427725252382686 1101 7 -0.29555853388890085 1101 8 -0.3248887670250653 1101 9 -0.5065882970656759 1101 10-11 -0.44268224145530155 1101 12-13 -0.4708359960891215 1101 14-15 -0.7326400467074379 1101 16-17 -0.6191582554844288 1101 18-19 -0.6626573374038927 1101 20-21 -0.6997821242668536 1101 22-23 -0.7378334724593358 1101 24-25 -0.6625857948026734 1101 26-27 -0.7961083898411729 1101 28-29 -0.8766213283377624 1101 30-31 -0.6295780575959 1101 32-33 -0.6145594922297377 1101 34-35 -0.6514015962772959 1101 36-37 -0.6103105783263771 1101 38-39 -0.6541934227275377 1101 40-41 -0.6227768376214229 1101 42-43 -0.6674141703111545 1101 44-45 -0.6812042097431643 1101 46-47 -0.6380969022684511 1101 48-49 -0.5401995598835683 1101 50-51 -0.537720975010842 1101 52-53 -0.5646752766245271 1101 54-55 -0.553984575432942 1101 56-57 -0.6434224209847272 1101 58-59 -0.5979068032591428 1101 60-61 -0.7150165159137245 1101 62-63 -0.8398673880062333 1101 64-65 -0.8251450931589659 1101 66-67 -0.766873199041008 1101 68-69 -0.8559673912011831 1101 70-71 -0.8773395957961867 1101 72-73 -0.919876820319935 1101 74-75 -0.8614620067654783 1101 76-77 -0.8736250046764624 1101 78-79 -0.75752419920844 1101 80-81 -0.7433115475854315 1101 82-83 -0.710858546291842 1101 84-85 -0.7099106763982128 1101 86-87 -0.7864733381243383 1101 88-89 -0.7857778993420439 1101 90-91 -0.8392351421341964 1101 92-93 -0.8202260665917933 1101 94-95 -0.8142544022548783 1101 96-97 -0.8729351849764733 1101 98-99 -1.0670244508772733 1101 100 -0.8772650951579024 1102 1 -0.3555772487098743 1102 2 -0.3567566663616013 1102 3 -0.3593529949185452 1102 4 -0.20969218969364078 1102 5 -0.32891689776676003 1102 6 -0.37557722178815567 1102 7 -0.27888807558059625 1102 8 -0.3413349011701925 1102 9 -0.43707179634647986 1102 10-11 -0.5271736396698259 1102 12-13 -0.4807421630220148 1102 14-15 -0.5916674253857792 1102 16-17 -0.6973147822855807 1102 18-19 -0.6227879959967808 1102 20-21 -0.7188730331367239 1102 22-23 -0.7137833678400085 1102 24-25 -0.7130105761835281 1102 26-27 -0.8214388792946963 1102 28-29 -0.8089321503421658 1102 30-31 -0.6786541046353847 1102 32-33 -0.634701788069151 1102 34-35 -0.6128060681559191 1102 36-37 -0.7088069459012729 1102 38-39 -0.7529684226066493 1102 40-41 -0.6381061597302065 1102 42-43 -0.618239219103387 1102 44-45 -0.6988638110429264 1102 46-47 -0.7366426746501702 1102 48-49 -0.7334117027867748 1102 50-51 -0.7329481248723226 1102 52-53 -0.8425527510286486 1102 54-55 -0.7455308409306767 1102 56-57 -0.8215952071340595 1102 58-59 -0.7691466935768219 1102 60-61 -0.7828215098529085 1102 62-63 -0.8123281356001755 1102 64-65 -0.7909007405910273 1102 66-67 -0.907413516483679 1102 68-69 -1.0024347793414705 1102 70-71 -0.9350921264907157 1102 72-73 -0.9040722800269663 1102 74-75 -1.0173023613897385 1102 76-77 -0.8470200603163356 1102 78-79 -0.7010861808243654 1102 80-81 -0.6628937259674323 1102 82-83 -0.6799781984258111 1102 84-85 -0.682093595402506 1102 86-87 -0.6497310244924392 1102 88-89 -0.7530764761134954 1102 90-91 -0.8279673003801094 1102 92-93 -0.9273341208679966 1102 94-95 -0.9651029397739883 1102 96-97 -1.028190003150712 1102 98-99 -1.0152213733177895 1102 100 -1.0293494755151826 1103 1 -0.34026895875700447 1103 2 -0.34118868108280154 1103 3 -0.398098362638617 1103 4 -0.300836214452616 1103 5 -0.26835019475895194 1103 6 -0.3202079500249013 1103 7 -0.3859525176995362 1103 8 -0.3691033404498967 1103 9 -0.5321286315396137 1103 10-11 -0.5140603424183396 1103 12-13 -0.5212836014424127 1103 14-15 -0.6682196316413993 1103 16-17 -0.6929249994532682 1103 18-19 -0.6396462538017218 1103 20-21 -0.6605204657384931 1103 22-23 -0.6972063588576347 1103 24-25 -0.8529316628362835 1103 26-27 -0.815923331404413 1103 28-29 -0.8752852784554221 1103 30-31 -0.8189741943173772 1103 32-33 -0.7160171145094196 1103 34-35 -0.8286367670604591 1103 36-37 -0.7925879802017164 1103 38-39 -0.8449008718414817 1103 40-41 -0.8294712293718973 1103 42-43 -0.7612823824112311 1103 44-45 -0.8147515943022654 1103 46-47 -0.8099908239867304 1103 48-49 -0.8695076109850817 1103 50-51 -0.8810253666081564 1103 52-53 -0.9350364552482375 1103 54-55 -0.9675846055608233 1103 56-57 -0.8882557764299648 1103 58-59 -0.8716403561600785 1103 60-61 -0.9122665437773847 1103 62-63 -0.9008034769010109 1103 64-65 -0.8424176692015521 1103 66-67 -0.8467286541282775 1103 68-69 -0.951793299475149 1103 70-71 -0.9887548434386133 1103 72-73 -0.8790534511029833 1103 74-75 -1.0259924111694048 1103 76-77 -0.9401748196889699 1103 78-79 -0.7648645371433673 1103 80-81 -0.7329112928774677 1103 82-83 -0.7968990302449299 1103 84-85 -0.8173144194834379 1103 86-87 -0.7873605328498918 1103 88-89 -0.8476989394755883 1103 90-91 -0.8070276530066565 1103 92-93 -0.912675595913143 1103 94-95 -0.9660218398812326 1103 96-97 -0.978078832483888 1103 98-99 -0.9431278035928337 1103 100 -1.1294956098428948 1104 1 -0.36743438577989807 1104 2 -0.3198363010309606 1104 3 -0.4085939030145447 1104 4 -0.2708414072788514 1104 5 -0.27912625281432213 1104 6 -0.36842239798895093 1104 7 -0.36618596335278397 1104 8 -0.3786621486719568 1104 9 -0.5334360019091378 1104 10-11 -0.5229598426501525 1104 12-13 -0.5607124061215245 1104 14-15 -0.6566395318589784 1104 16-17 -0.6608868375308674 1104 18-19 -0.7176227170103786 1104 20-21 -0.7307849496670897 1104 22-23 -0.6825660715147563 1104 24-25 -0.7690322063806292 1104 26-27 -0.8024729775665165 1104 28-29 -0.8211917301480369 1104 30-31 -0.829738967849643 1104 32-33 -0.7121675356458752 1104 34-35 -0.7550834088739364 1104 36-37 -0.7532852119720062 1104 38-39 -0.7461205342280053 1104 40-41 -0.8083301617431644 1104 42-43 -0.7304203596371153 1104 44-45 -0.8089305119725196 1104 46-47 -0.8576727591968378 1104 48-49 -0.8769035263379337 1104 50-51 -0.7944595329922208 1104 52-53 -0.7901932585607483 1104 54-55 -0.8665087819316923 1104 56-57 -0.8401258261966618 1104 58-59 -0.873671473037966 1104 60-61 -0.8769292931580495 1104 62-63 -0.8659801973968015 1104 64-65 -0.8453315665392438 1104 66-67 -0.9185943729728265 1104 68-69 -0.9522338433658177 1104 70-71 -0.9428142268795128 1104 72-73 -0.9453422613504188 1104 74-75 -0.9805130981322563 1104 76-77 -0.9035403936830448 1104 78-79 -0.6579782931132243 1104 80-81 -0.7178927599938802 1104 82-83 -0.6447749036037855 1104 84-85 -0.6749040686962537 1104 86-87 -0.8154147328034682 1104 88-89 -0.8118081635420502 1104 90-91 -0.7367750567394609 1104 92-93 -0.7936566995900876 1104 94-95 -0.8089844902499337 1104 96-97 -0.8372519726728775 1104 98-99 -0.9126242006197138 1104 100 -1.1107151282544692 1105 1 -0.4041479905620129 1105 2 -0.36672745769113035 1105 3 -0.4474571110131649 1105 4 -0.24054273878557098 1105 5 -0.29895756120899364 1105 6 -0.42283876061478054 1105 7 -0.4112228466143151 1105 8 -0.42224235136046673 1105 9 -0.5639911977624692 1105 10-11 -0.5515932831176116 1105 12-13 -0.5492981425094783 1105 14-15 -0.791262465760461 1105 16-17 -0.651501326010596 1105 18-19 -0.7178347627354569 1105 20-21 -0.7815111645168358 1105 22-23 -0.7251790491947006 1105 24-25 -0.7636896197578622 1105 26-27 -0.8435552436683693 1105 28-29 -0.8220270419276687 1105 30-31 -0.7235282701506947 1105 32-33 -0.723435987487818 1105 34-35 -0.7201531035341446 1105 36-37 -0.6792478280856571 1105 38-39 -0.7406376637356331 1105 40-41 -0.7997083766364739 1105 42-43 -0.7854251253554949 1105 44-45 -0.75765540874891 1105 46-47 -0.7598445332653796 1105 48-49 -0.8757650939935289 1105 50-51 -0.8752576904730205 1105 52-53 -0.943534363227144 1105 54-55 -0.8082322365878909 1105 56-57 -0.8074810650428077 1105 58-59 -0.9440406694736083 1105 60-61 -0.8017987716741928 1105 62-63 -0.8396252667515895 1105 64-65 -0.8760133620295676 1105 66-67 -0.9062974795091989 1105 68-69 -0.8609610897205258 1105 70-71 -0.9574526620981629 1105 72-73 -0.9181762105813718 1105 74-75 -0.9575609463783934 1105 76-77 -0.9970158549482093 1105 78-79 -0.8782415921198705 1105 80-81 -0.8552166689439034 1105 82-83 -0.8508364778348785 1105 84-85 -0.8375562504734901 1105 86-87 -0.8981808921992283 1105 88-89 -0.8445240436256647 1105 90-91 -0.9167767212867446 1105 92-93 -0.9458031159120708 1105 94-95 -0.8549521156373423 1105 96-97 -0.9694080132146148 1105 98-99 -1.073698434688442 1105 100 -1.0772634764194677 1106 1 -0.42954041140217925 1106 2 -0.38417631522769113 1106 3 -0.4517116867755959 1106 4 -0.29779216415423804 1106 5 -0.32883874052829043 1106 6 -0.3593795470564558 1106 7 -0.4244811806072306 1106 8 -0.47046507927404235 1106 9 -0.5462608406680332 1106 10-11 -0.5172080608412699 1106 12-13 -0.5763622874857788 1106 14-15 -0.6991816539490614 1106 16-17 -0.6462254982197848 1106 18-19 -0.6942470922710058 1106 20-21 -0.72483515097084 1106 22-23 -0.6909199176072036 1106 24-25 -0.6977160095221535 1106 26-27 -0.7419835778283854 1106 28-29 -0.7097861538529813 1106 30-31 -0.768677091935082 1106 32-33 -0.7596413446798067 1106 34-35 -0.8010544954872927 1106 36-37 -0.8884998102397859 1106 38-39 -0.895428899781102 1106 40-41 -0.8547634913130864 1106 42-43 -0.7875545207936057 1106 44-45 -0.8468343210924516 1106 46-47 -0.8598617101667756 1106 48-49 -0.8279871919164563 1106 50-51 -0.8928143711206928 1106 52-53 -0.9141737004301405 1106 54-55 -0.904252434962892 1106 56-57 -0.9439539231120975 1106 58-59 -0.9267454884818775 1106 60-61 -0.9342941445549187 1106 62-63 -1.022425902675927 1106 64-65 -0.9438023667413944 1106 66-67 -0.9605013225622727 1106 68-69 -0.9583187674665012 1106 70-71 -0.9387197458167762 1106 72-73 -0.976183186957428 1106 74-75 -0.9556216375629951 1106 76-77 -0.9025217216361412 1106 78-79 -0.7096433693619346 1106 80-81 -0.7271541761421147 1106 82-83 -0.791503635539371 1106 84-85 -0.8347904291376764 1106 86-87 -0.8095087441001922 1106 88-89 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0.5454859294922585 2310 64-65 0.7152262886248835 2310 66-67 1.0041525005599397 2310 68-69 1.164962060466685 2310 70-71 1.1659986118433778 2310 72-73 1.1837974349367926 2310 74-75 1.1766163582501434 2310 76-77 1.204104398402464 2310 78-79 1.0549232818582652 2310 80-81 0.9965280983877847 2310 82-83 1.0079293034876144 2310 84-85 1.0861871658070896 2310 86-87 1.0456007194560755 2310 88-89 1.0564575105242007 2310 90-91 1.135837489091685 2310 92-93 1.1718443152937823 2310 94-95 1.1685400410780495 2310 96-97 1.2345711345286219 2310 98-99 1.220726600762351 2310 100 1.4522358341687216 2311 1 0.4167640257009708 2311 2 0.3648469683856348 2311 3 0.41794774399446055 2311 4 0.3085201260259396 2311 5 0.3982813008549613 2311 6 0.4629365197552531 2311 7 0.4935228804097065 2311 8 0.5024400597089667 2311 9 0.6212827877011975 2311 10-11 0.6491478107976576 2311 12-13 0.701521560234049 2311 14-15 0.8280359385049323 2311 16-17 0.7345640613227076 2311 18-19 0.8214366624870806 2311 20-21 0.779256000199446 2311 22-23 0.9033228931441855 2311 24-25 0.9279035017907873 2311 26-27 0.3348847543844542 2311 28-29 0.629046670749112 2311 30-31 0.8802779207996281 2311 32-33 0.9292720735142694 2311 34-35 0.9928208624329216 2311 36-37 0.9985518667020727 2311 38-39 1.0852236295362374 2311 40-41 1.0664823495194256 2311 42-43 1.0560357386438923 2311 44-45 1.0391443684257808 2311 46-47 0.6810733987705007 2311 48-49 0.9682312371395341 2311 50-51 1.0620622741551742 2311 52-53 1.0045832569459563 2311 54-55 1.0118104927582792 2311 56-57 1.1447942948190715 2311 58-59 1.1118113496811972 2311 60-61 1.1014718481661205 2311 62-63 1.1555102988907677 2311 64-65 1.18167505514063 2311 66-67 1.237723368156729 2311 68-69 1.25801447199769 2311 70-71 1.2786406704223339 2311 72-73 1.2891714070332583 2311 74-75 1.2576526929078184 2311 76-77 1.2604779778166773 2311 78-79 0.9563357830132091 2311 80-81 0.8736659067588448 2311 82-83 0.9269114479946836 2311 84-85 0.9926104079765423 2311 86-87 1.0292749548806412 2311 88-89 1.0797741741110123 2311 90-91 1.0978050581575616 2311 92-93 1.2028402267785765 2311 94-95 1.2195431132391938 2311 96-97 1.2681847351938735 2311 98-99 1.2974630647741812 2311 100 1.4192736240606187 2312 1 0.23850964005645636 2312 2 0.355243032672206 2312 3 0.42137345628525935 2312 4 0.16931992009337904 2312 5 0.32635146249322844 2312 6 0.4293264652527782 2312 7 0.3139878452782483 2312 8 0.3982686114981888 2312 9 0.575121306929077 2312 10-11 0.6208319519538179 2312 12-13 0.6523613651372742 2312 14-15 0.8338131138791383 2312 16-17 0.8041173115700957 2312 18-19 0.7708655198075647 2312 20-21 0.7878724741494594 2312 22-23 0.8958077110816802 2312 24-25 0.8808228122585717 2312 26-27 0.9076459298038984 2312 28-29 0.9363579824683441 2312 30-31 0.926395882415946 2312 32-33 0.7446251001833275 2312 34-35 0.8955733735647513 2312 36-37 0.9561923486417498 2312 38-39 0.7428643453355761 2312 40-41 0.8775817029229884 2312 42-43 0.9379465752708498 2312 44-45 0.6935581398851127 2312 46-47 0.7449686527758885 2312 48-49 0.8855158212513885 2312 50-51 0.7302387713533847 2312 52-53 0.9055282710559851 2312 54-55 0.7608022196115556 2312 56-57 0.8342985161771921 2312 58-59 0.9153362269697212 2312 60-61 0.9226187562927421 2312 62-63 0.9456497893718065 2312 64-65 0.9420022316012222 2312 66-67 0.9073846445567639 2312 68-69 0.9685993299081801 2312 70-71 0.8730243692308193 2312 72-73 0.8855625868845536 2312 74-75 0.975795295338024 2312 76-77 0.9818014627388294 2312 78-79 0.8432433160536519 2312 80-81 0.7716352948701584 2312 82-83 0.8909445814006887 2312 84-85 0.9148066741392711 2312 86-87 0.9526000871101985 2312 88-89 0.9292613262655003 2312 90-91 0.9799318152780216 2312 92-93 1.0039560859965988 2312 94-95 1.0523067020902772 2312 96-97 0.6935366737777358 2312 98-99 0.46484286590758117 2312 100 0.591338322907422 2313 1 0.05061325145561568 2313 2 0.2476227864452838 2313 3 0.2834368286215607 2313 4 0.19620127266060194 2313 5 0.30909621959460054 2313 6 0.2530943466333184 2313 7 0.24844842523070554 2313 8 0.432874279821867 2313 9 0.6351637235965057 2313 10-11 0.4197271291331788 2313 12-13 0.44645174323593295 2313 14-15 0.5123848000192339 2313 16-17 0.7388672307247717 2313 18-19 0.6994533915155472 2313 20-21 0.6798405748417551 2313 22-23 0.6586904700563352 2313 24-25 0.5799640694815267 2313 26-27 0.8122057380558729 2313 28-29 0.6374309787606336 2313 30-31 0.8525646450373259 2313 32-33 0.9085936495991547 2313 34-35 0.9636105365858114 2313 36-37 1.0179039458342558 2313 38-39 1.0542314824522947 2313 40-41 0.9964450601753256 2313 42-43 1.0269359291032103 2313 44-45 0.8977954662719014 2313 46-47 0.9256375107448278 2313 48-49 1.0460974075294658 2313 50-51 0.9885367815311099 2313 52-53 1.0188565230369306 2313 54-55 0.9835005167478883 2313 56-57 0.6781762415763808 2313 58-59 0.7113604524286146 2313 60-61 0.6497695822683127 2313 62-63 0.9724572444864563 2313 64-65 1.0609742380365077 2313 66-67 0.990488537253917 2313 68-69 1.1466172864404172 2313 70-71 1.0878090912759717 2313 72-73 1.0702803535715972 2313 74-75 1.1254505217003938 2313 76-77 1.085569486047362 2313 78-79 1.0096529032568924 2313 80-81 0.8496403169758509 2313 82-83 0.9227741710942752 2313 84-85 0.9423204520884667 2313 86-87 0.9675717497826639 2313 88-89 0.9834375782292817 2313 90-91 0.8221719061251491 2313 92-93 1.0313471498632936 2313 94-95 1.1008619782182087 2313 96-97 1.2030043109348512 2313 98-99 1.1906286374772783 2313 100 1.2484292065833174 2314 1 -0.37589994807559535 2314 2 0.03093272343642184 2314 3 0.25507321893831403 2314 4 0.21128773475934537 2314 5 0.3112715026518842 2314 6 0.37414149675637987 2314 7 0.43429981000146256 2314 8 0.44649900217912375 2314 9 0.43667555183446893 2314 10-11 0.5886801908837498 2314 12-13 0.47437648807533606 2314 14-15 0.7443034060162788 2314 16-17 0.7944453377437171 2314 18-19 0.8161679533942205 2314 20-21 0.8601188316239714 2314 22-23 0.9243570146994884 2314 24-25 0.8531737353136535 2314 26-27 0.7408120293687759 2314 28-29 0.7888514345322122 2314 30-31 0.7900833497517894 2314 32-33 0.8653000033363085 2314 34-35 0.8923091027898451 2314 36-37 0.8359361106060206 2314 38-39 0.5983805724354241 2314 40-41 0.6424287364889025 2314 42-43 0.8322380236310281 2314 44-45 0.8312914714730084 2314 46-47 0.8061366575610904 2314 48-49 0.8879911576103794 2314 50-51 0.9125865223978877 2314 52-53 0.9048168343970104 2314 54-55 0.7880815307515618 2314 56-57 0.7677972035689962 2314 58-59 0.8828924596684189 2314 60-61 0.9195383614328065 2314 62-63 0.8829565207810575 2314 64-65 0.9166146672924924 2314 66-67 0.5832200866897281 2314 68-69 0.5776408087297185 2314 70-71 0.7108534250491445 2314 72-73 0.8725405469494447 2314 74-75 0.9398739370327505 2314 76-77 0.8156413779102039 2314 78-79 0.73329464220582 2314 80-81 0.7751336902355987 2314 82-83 0.8052764300988073 2314 84-85 0.8314575143192826 2314 86-87 0.8620651854274257 2314 88-89 0.8958252332158274 2314 90-91 0.9838214051420948 2314 92-93 0.8706687854178838 2314 94-95 0.8520590838674273 2314 96-97 0.8572525381606901 2314 98-99 1.0032692264232033 2314 100 1.0834535504714893 2315 1 0.3994468983535313 2315 2 0.4233062932786922 2315 3 0.4560289620232254 2315 4 0.3051326926151603 2315 5 0.35990203596625037 2315 6 0.43096603252330823 2315 7 0.3890633903440204 2315 8 0.2704569595593611 2315 9 0.5043127069152078 2315 10-11 0.5897542844348038 2315 12-13 0.4282582362953846 2315 14-15 0.6402827118683874 2315 16-17 0.8261536206059787 2315 18-19 0.7606260961470568 2315 20-21 0.5730566368623613 2315 22-23 0.886425154631894 2315 24-25 0.9161811373536892 2315 26-27 0.8694715464707627 2315 28-29 0.824315410329902 2315 30-31 0.8868129087032486 2315 32-33 0.791786654109508 2315 34-35 0.858286843870701 2315 36-37 0.823880675080261 2315 38-39 0.8806207066486351 2315 40-41 0.8831409282073679 2315 42-43 0.9029080394841813 2315 44-45 0.9600864497668482 2315 46-47 0.9939949081007526 2315 48-49 0.8720707639561809 2315 50-51 0.9400489760475352 2315 52-53 1.0097884486783926 2315 54-55 0.7791059682546901 2315 56-57 0.7755888889313169 2315 58-59 0.7828858857091703 2315 60-61 0.8911447863259951 2315 62-63 0.9629300329475825 2315 64-65 1.0106694004951464 2315 66-67 1.034911531461269 2315 68-69 1.0127213752498747 2315 70-71 0.952667004537318 2315 72-73 1.000466687442092 2315 74-75 0.42993178365021834 2315 76-77 0.48911610512641346 2315 78-79 0.7167775427119025 2315 80-81 0.7839912401801215 2315 82-83 0.7016626916759492 2315 84-85 0.7133456805974419 2315 86-87 0.785293498609775 2315 88-89 0.7725089357295793 2315 90-91 0.8612836303159384 2315 92-93 0.8613490807622206 2315 94-95 0.9211614369954084 2315 96-97 1.0160540961736721 2315 98-99 1.0277383744138895 2315 100 0.877487805330297 2316 1 0.2825583144050654 2316 2 0.07079465059964463 2316 3 0.21945537750038113 2316 4 0.04378447156831555 2316 5 0.29302239283651943 2316 6 0.34640499564898874 2316 7 0.4191173807068225 2316 8 0.4136573840064983 2316 9 0.6078552105734119 2316 10-11 0.589456921917197 2316 12-13 0.37214157186382835 2316 14-15 0.5056561974976503 2316 16-17 0.7722165479070995 2316 18-19 0.7529520615237715 2316 20-21 0.7419261623385225 2316 22-23 0.7731969649867878 2316 24-25 0.49129893736546393 2316 26-27 0.699112135761041 2316 28-29 0.8835489973029738 2316 30-31 0.5946870409601388 2316 32-33 0.6866293341561232 2316 34-35 0.7879016097976859 2316 36-37 0.7889758393182618 2316 38-39 0.7310627776296528 2316 40-41 0.6948764969981553 2316 42-43 0.6813310444793998 2316 44-45 0.506668880648725 2316 46-47 0.7050112552165402 2316 48-49 0.7247175953084479 2316 50-51 0.7889386035050734 2316 52-53 0.7777315873747455 2316 54-55 0.7226788854607591 2316 56-57 0.8370778711549391 2316 58-59 0.7022464991721691 2316 60-61 0.8024797115138824 2316 62-63 0.8699617586580857 2316 64-65 0.9402113698454144 2316 66-67 0.8972944517819599 2316 68-69 0.9262236209131487 2316 70-71 0.9266722108124412 2316 72-73 0.8895217207806141 2316 74-75 0.9169087060416174 2316 76-77 0.7643644875486046 2316 78-79 0.5605875228299908 2316 80-81 0.5694509396821914 2316 82-83 0.4756467326410956 2316 84-85 0.5748913146746446 2316 86-87 0.6495365482875215 2316 88-89 0.631571447075661 2316 90-91 0.46150536169302114 2316 92-93 0.30973320430770457 2316 94-95 0.3632487977263956 2316 96-97 0.5714556303799796 2316 98-99 0.7408812086717482 2316 100 0.3379780788148423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 1.0 11 5.0 12 34.0 13 218.0 14 802.0 15 2423.0 16 5905.0 17 12492.0 18 21632.0 19 31788.0 20 45611.0 21 65610.0 22 80620.0 23 80041.0 24 77022.0 25 80826.0 26 93653.0 27 113763.0 28 140935.0 29 173693.0 30 215439.0 31 268713.0 32 342552.0 33 456539.0 34 666884.0 35 934783.0 36 1474485.0 37 2131945.0 38 1703076.0 39 300297.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.90529585536455 21.571777460516124 13.72085438740786 22.80207229671147 2 15.273673462914974 20.24304802169165 41.375169584140515 23.108108931252865 3 18.663854459787213 26.982577494097242 29.239992093950086 25.113575952165455 4 12.227485592556727 16.852984513771244 39.661171643605165 31.258358250066863 5 12.726492891801591 38.767842961147565 34.63853871664478 13.867125430406071 6 29.526197822223523 38.38914443055121 17.968031748545606 14.116625998679663 7 26.925540920041925 32.4009372236587 23.054399721059387 17.619122135239987 8 26.384341148154206 35.48654868784566 19.684079370523836 18.445030793476292 9 26.41846614252393 15.3741814274156 21.840226059269447 36.36712637079102 10-11 25.819464066142313 26.49528365098429 26.978670566781055 20.70658171609234 12-13 25.79350511737804 24.100257309015362 27.946612514551443 22.159625059055152 14-15 23.10899641024095 25.331320970626486 25.82075527875008 25.738927340382485 16-17 21.49092274570723 28.006406775529552 27.098852007360584 23.403818471402634 18-19 20.94026752442967 27.782629206889187 29.741298461575948 21.535804807105194 20-21 22.557833212785663 26.916526786210326 29.155977622347656 21.36966237865635 22-23 22.798383544661935 26.688951967386075 28.851122190689978 21.661542297262017 24-25 22.140577742343194 26.6754118889969 29.687645800746825 21.49636456791308 26-27 22.03497158272061 26.983579032845768 28.72367234228086 22.257777042152764 28-29 21.31247096794588 27.378029140069376 29.300375149538382 22.00912474244636 30-31 22.317676754840427 26.909756975156853 29.210473306000328 21.562092964002396 32-33 22.52904798122846 27.220483785870066 28.384556312890812 21.865911920010664 34-35 22.43750742241258 27.099940694234103 29.149443124058198 21.31310875929512 36-37 22.16165738671399 26.713515277870943 28.78824274005072 22.33658459536435 38-39 22.271528203248163 26.794468413764417 29.353306317404687 21.580697065582736 40-41 21.92440692263518 25.972710522457565 29.91019348241888 22.192689072488378 42-43 22.021272523348625 26.228160213387923 29.105159432076672 22.645407831186777 44-45 22.031182285888704 27.111918775942083 28.93059835983096 21.92630057833825 46-47 22.0869033160234 26.882512588546557 28.293926641763118 22.73665745366693 48-49 22.839173721326034 26.866113846737143 28.273630636397577 22.021081795539246 50-51 22.46251527918735 26.68745642723693 28.18686101486627 22.663167278709444 52-53 21.89473825729354 27.23854889280769 27.93464269150877 22.93207015839 54-55 21.926619067362793 27.19897676638958 27.85474641173704 23.019657754510586 56-57 22.84774064662117 26.681538196720293 27.525404144795303 22.94531701186323 58-59 21.809015289670636 27.197255373199653 28.28453577842587 22.70919355870385 60-61 22.05014568796089 27.80106280845722 27.531884306230076 22.616907197351814 62-63 22.39034074681187 27.080622307692593 27.25284491295845 23.276192032537086 64-65 21.58932142085134 27.216036044223195 27.610234277470024 23.584408257455436 66-67 22.082207238685967 26.86972870533041 27.62401157806397 23.424052477919645 68-69 21.70992334485034 26.9025269680697 27.826257014619536 23.561292672460425 70-71 21.67922781325717 26.502142782799964 28.005834156928227 23.812795247014634 72-73 22.177363958882914 26.7071863799462 28.064357385195038 23.051092275975844 74-75 22.332054886167914 27.05284694207527 27.587353143696188 23.027745028060632 76-77 22.010095362011363 26.927580393492722 27.34799373130495 23.71433051319097 78-79 21.871864927130023 26.9557103847321 27.45461204899461 23.717812639143272 80-81 21.485486829655187 27.411620818038685 27.823537223631018 23.279355128675107 82-83 22.440014219334227 26.363243472793442 28.782589542065256 22.41415276580707 84-85 22.526431386625386 26.853107731700725 27.534950081519717 23.08551080015417 86-87 21.268922935754848 27.529034315338063 27.968453572811363 23.233589176095723 88-89 22.10582111676117 26.400249964687127 27.894273471288955 23.599655447262748 90-91 21.935601860379492 26.394704077962523 27.98657936028676 23.68311470137122 92-93 22.200091626445044 26.66514463734981 27.75225915501422 23.382504581190922 94-95 21.04461317223024 26.58882190264848 28.45100309026946 23.91556183485182 96-97 21.38710423780196 26.560019257532634 28.026662692489996 24.026213812175413 98-99 20.759386351640888 27.09779360906475 27.88556639969184 24.25725363960252 100 21.33600620579297 26.539682112793482 27.27116768751849 24.85314399389506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1379.0 1 986.5 2 1059.5 3 1425.0 4 1034.5 5 832.0 6 1334.5 7 2546.5 8 3991.0 9 4781.5 10 4501.5 11 4171.0 12 4647.5 13 5573.0 14 6697.0 15 8053.0 16 9567.5 17 10962.5 18 12311.5 19 13601.5 20 14973.5 21 16191.0 22 17767.0 23 20193.0 24 22941.0 25 26381.5 26 31695.5 27 37940.5 28 46644.0 29 63728.0 30 88279.5 31 112223.0 32 145062.5 33 180885.0 34 207893.0 35 236318.0 36 264884.5 37 307370.5 38 326322.5 39 309874.5 40 287619.0 41 264492.0 42 258485.0 43 271139.0 44 295859.5 45 344857.0 46 387748.0 47 414862.0 48 437081.5 49 432101.5 50 411394.5 51 411873.5 52 409099.5 53 381250.5 54 358308.5 55 312657.5 56 267976.5 57 219532.5 58 172000.0 59 162850.5 60 133714.0 61 89282.5 62 65426.0 63 54752.0 64 39426.0 65 19045.0 66 10311.5 67 7000.5 68 5338.0 69 4168.0 70 3370.5 71 2711.5 72 2249.0 73 2054.0 74 1659.0 75 1268.5 76 923.0 77 567.0 78 364.5 79 247.5 80 161.0 81 108.5 82 70.0 83 32.5 84 16.0 85 11.5 86 8.5 87 4.5 88 2.0 89 1.0 90 0.5 91 1.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04480234980238026 2 0.0 3 0.0 4 0.029238101699443655 5 0.0 6 0.0 7 8.611797195956825E-4 8 0.0 9 0.011898983198803758 10-11 5.251095851193185E-4 12-13 2.625547925596593E-5 14-15 1.3652849213102283E-4 16-17 2.888102718156252E-4 18-19 0.0 20-21 1.0502191702386372E-5 22-23 0.0 24-25 5.408628726728981E-4 26-27 6.563869813991483E-4 28-29 1.9954164234534103E-4 30-31 0.002562534775382275 32-33 0.015259684543567398 34-35 0.026376254460543373 36-37 0.03156433716152224 38-39 0.022364417230231776 40-41 0.0013652849213102282 42-43 8.926862947028415E-5 44-45 0.011510402105815462 46-47 0.018478606300348817 48-49 0.003539238603704207 50-51 0.011652181693797678 52-53 0.011032552383356884 54-55 0.010759495399094838 56-57 5.146073934169322E-4 58-59 0.008601295004254437 60-61 0.004673475307561935 62-63 0.0014020425922685806 64-65 0.0369099527380369 66-67 0.06192092227727005 68-69 0.061884164606311694 70-71 0.05365044631164078 72-73 0.04790574745043543 74-75 0.03679967972516184 76-77 0.03249903222303462 78-79 0.015847807278901033 80-81 0.0011394877997089213 82-83 0.03986106860640747 84-85 0.05362419083238481 86-87 0.05908007942177453 88-89 0.07223407452901347 90-91 0.050820105647847655 92-93 0.040197138740883834 94-95 0.013579333871185578 96-97 0.0037072736709423885 98-99 0.022427430380446093 100 0.03123351812289707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9521822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.87536406458533 #Duplication Level Percentage of deduplicated Percentage of total 1 74.2269278710681 45.92818185418054 2 15.492661204721589 19.172281047428505 3 5.155539524352076 9.570026550559312 4 2.219688434606977 5.4937612000502485 5 1.0655633023346474 3.296605863290905 6 0.573811181157018 2.130286544305213 7 0.35052474333973366 1.5182192275453943 8 0.2252638418778824 1.1150625781424923 9 0.14181854938617966 0.7897566936943117 >10 0.502786900230415 5.032398179608558 >50 0.023594992980776254 1.0160065460440637 >100 0.01929697740785749 2.3134074300156207 >500 0.001689227093448381 0.7196910718685589 >1k 7.431684255878291E-4 0.7754543555566038 >5k 2.2520254451410833E-5 0.09425288650507083 >10k+ 6.75607633542325E-5 1.0346079712046863 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31379 0.3295482734291819 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26634 0.27971537380135864 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16235 0.17050308228824274 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.050219170238637E-5 0.0 7 0.0 0.0 0.0 1.050219170238637E-5 0.0 8 0.0 0.0 0.0 1.050219170238637E-5 0.0 9 0.0 0.0 0.0 4.200876680954548E-5 0.0 10-11 0.0 0.0 0.0 5.776205436312504E-5 0.0 12-13 0.0 0.0 0.0 7.35153419167046E-5 0.0 14-15 0.0 0.0 0.0 7.35153419167046E-5 0.0 16-17 0.0 0.0 0.0 7.876643776789778E-5 0.0 18-19 0.0 0.0 0.0 1.102730128750569E-4 0.0 20-21 0.0 0.0 0.0 2.100438340477274E-4 0.0 22-23 0.0 0.0 0.0 4.3584095564903435E-4 0.0 24-25 0.0 0.0 0.0 0.0010029593075778984 0.0 26-27 0.0 0.0 0.0 0.0021687025865427855 0.0 28-29 0.0 0.0 0.0 0.0054033776308777875 0.0 30-31 0.0 0.0 0.0 0.018105778494914106 0.0 32-33 0.0 0.0 0.0 0.047895245258733045 0.0 34-35 0.0 0.0 0.0 0.09152660068629723 0.0 36-37 0.0 0.0 0.0 0.16448007534692416 0.0 38-39 0.0 0.0 0.0 0.2697750493550499 0.0 40-41 0.0 0.0 0.0 0.3807464579783155 0.0 42-43 0.0 0.0 0.0 0.48837816964022224 0.0 44-45 0.0 0.0 0.0 0.6165206616968895 0.0 46-47 5.251095851193185E-6 0.0 0.0 0.7674424075560329 0.0 48-49 1.050219170238637E-5 0.0 0.0 0.9322322975581774 0.0 50-51 1.050219170238637E-5 0.0 0.0 1.1038906209336825 0.0 52-53 1.050219170238637E-5 0.0 0.0 1.2698515053106432 0.0 54-55 1.050219170238637E-5 0.0 0.0 1.447312289601717 0.0 56-57 1.050219170238637E-5 0.0 0.0 1.6532077579270017 0.0 58-59 1.050219170238637E-5 1.050219170238637E-5 0.0 1.8925317024409822 0.0 60-61 1.050219170238637E-5 1.050219170238637E-5 0.0 2.1345389569349225 0.0 62-63 1.050219170238637E-5 1.5753287553579557E-5 0.0 2.379770384281496 0.0 64-65 1.050219170238637E-5 2.100438340477274E-5 0.0 2.6257527183347893 0.0 66-67 1.050219170238637E-5 2.100438340477274E-5 0.0 2.9036039531089743 0.0 68-69 1.050219170238637E-5 2.100438340477274E-5 0.0 3.205494704689922 0.0 70-71 1.050219170238637E-5 2.100438340477274E-5 0.0 3.5342448115497223 0.0 72-73 1.050219170238637E-5 2.100438340477274E-5 0.0 3.8638508470332678 0.0 74-75 1.050219170238637E-5 2.100438340477274E-5 0.0 4.182167026436747 0.0 76-77 1.050219170238637E-5 2.100438340477274E-5 0.0 4.536594992008882 0.0 78-79 1.050219170238637E-5 2.100438340477274E-5 0.0 4.9229233648770165 0.0 80-81 1.050219170238637E-5 2.100438340477274E-5 0.0 5.334635535089818 0.0 82-83 1.050219170238637E-5 2.100438340477274E-5 0.0 5.752354958956385 0.0 84-85 1.050219170238637E-5 2.100438340477274E-5 0.0 6.1549774822507715 1.050219170238637E-5 86-87 1.050219170238637E-5 2.100438340477274E-5 0.0 6.5788091816881265 1.050219170238637E-5 88 1.050219170238637E-5 2.100438340477274E-5 0.0 6.917321075735295 1.050219170238637E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19695 0.0 47.460213 1 GTATCAA 34380 0.0 39.113747 1 GGGCACC 9450 0.0 35.468105 94 CAACGCA 39785 0.0 32.065414 5 ATCAACG 40025 0.0 31.930662 3 GTGGTAT 5930 0.0 30.68496 1 TATCAAC 44405 0.0 30.281017 2 TAGGCAT 11865 0.0 30.137228 5 TACCTGG 4785 0.0 29.073292 2 AACGCAG 45420 0.0 29.05969 6 CTGTGCG 12345 0.0 28.775118 9 CTAGGCA 13295 0.0 27.647032 4 TCAACGC 47205 0.0 27.302948 4 TAGTACT 13315 0.0 27.146587 4 ACTGTGC 14175 0.0 26.783953 8 TACCCCG 635 0.0 26.638832 5 GCATCGT 11745 0.0 26.484472 8 ACGCAGA 48225 0.0 26.45369 7 CGCAGAG 48525 0.0 26.299688 8 ACCTGGG 5085 0.0 26.251703 3 >>END_MODULE