FastQCFastQC Report
Mon 6 Jun 2016
ERR1211188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5950647
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211680.35572602441381584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163000.2739197939316515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104590.17576240029025414No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94500.048.6530461
GTATCAA171050.041.488291
TACCTGG53250.040.4752462
GTACCTG64050.034.8997041
ATCAACG204950.033.785253
CAACGCA206300.033.518595
TATCAAC217150.032.3551642
ACCTGGG66150.032.191463
AACGCAG225450.031.2143886
GTACTAG30600.031.0654181
TCAACGC224550.031.0037564
GTACATG207500.029.3696611
GTGGTAT41050.028.7745021
TACATGG211250.028.1574922
GCATCGT28950.027.7652518
ACGCAGA250550.027.7498557
CGCAGAG255850.027.154588
ACATGGG212300.027.0800083
CATGGGG164700.025.8587154
ATGGGGG128400.024.78545
Produced by FastQC (version 0.11.5)