##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211188_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5950647 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39422637572015 31.0 31.0 34.0 28.0 34.0 2 31.394682796677404 31.0 31.0 34.0 28.0 34.0 3 31.45899580331349 31.0 31.0 34.0 28.0 34.0 4 35.058717144539074 37.0 35.0 37.0 32.0 37.0 5 35.11895059478406 37.0 35.0 37.0 32.0 37.0 6 35.18390369988339 37.0 35.0 37.0 32.0 37.0 7 35.10568346601639 37.0 35.0 37.0 32.0 37.0 8 35.10321768372414 37.0 35.0 37.0 32.0 37.0 9 36.66710762712021 39.0 37.0 39.0 32.0 39.0 10-11 36.61339691297434 39.0 37.0 39.0 32.0 39.0 12-13 36.506547943442115 39.0 37.0 39.0 32.0 39.0 14-15 37.69732568576157 40.0 37.0 41.0 32.0 41.0 16-17 37.66558115445262 40.0 37.0 41.0 32.0 41.0 18-19 37.68345921040183 40.0 37.0 41.0 32.0 41.0 20-21 37.64645651136759 40.0 37.0 41.0 32.0 41.0 22-23 37.53420384371649 40.0 37.0 41.0 32.0 41.0 24-25 37.44414229242635 40.0 37.0 41.0 31.0 41.0 26-27 37.29462140839475 40.0 36.5 41.0 31.0 41.0 28-29 37.16307856943959 40.0 36.0 41.0 31.0 41.0 30-31 37.00316629435421 39.5 36.0 41.0 30.0 41.0 32-33 36.79717894541551 39.0 35.5 41.0 30.0 41.0 34-35 36.63217562728893 39.0 35.0 41.0 30.0 41.0 36-37 36.51316831598312 39.0 35.0 41.0 30.0 41.0 38-39 36.366823557169496 39.0 35.0 41.0 29.0 41.0 40-41 36.2011209873481 39.0 35.0 40.0 28.5 41.0 42-43 35.99347827219461 39.0 35.0 40.0 28.0 41.0 44-45 35.783788048593706 38.0 35.0 40.0 27.0 41.0 46-47 35.5520278719272 38.0 34.0 40.0 26.0 41.0 48-49 35.348275153945444 38.0 34.0 40.0 26.0 41.0 50-51 34.67724232339778 37.5 33.5 39.5 24.5 40.5 52-53 34.78922249967104 38.0 33.5 40.0 25.0 40.5 54-55 35.274842634758876 38.0 34.0 40.0 26.0 41.0 56-57 35.24606979711618 38.0 34.0 40.0 26.0 41.0 58-59 35.04954948596346 38.0 34.0 40.0 26.0 41.0 60-61 34.82781519387724 37.5 34.0 40.0 26.0 41.0 62-63 34.52913405886788 37.0 33.0 40.0 25.0 41.0 64-65 34.28412322223113 36.5 33.0 40.0 25.0 41.0 66-67 34.11094323020673 36.0 33.0 39.0 25.0 41.0 68-69 33.81301478645935 36.0 33.0 39.0 24.0 41.0 70-71 33.49699772142424 35.0 32.5 39.0 24.0 41.0 72-73 33.147080309082355 35.0 32.0 38.0 23.0 40.0 74-75 32.814625367628096 35.0 32.0 37.0 23.0 39.5 76-77 32.476072433804255 35.0 32.0 37.0 22.0 39.0 78-79 32.132687504400785 35.0 31.0 36.0 21.5 39.0 80-81 31.82992160348278 35.0 31.0 36.0 20.5 38.0 82-83 31.53607515283632 35.0 31.0 35.5 20.0 37.0 84-85 31.280931300411538 34.5 31.0 35.0 20.0 37.0 86-87 31.046177079567986 34.0 30.5 35.0 19.5 36.5 88-89 30.821620741408456 34.0 30.0 35.0 19.0 36.0 90-91 30.59692912384149 34.0 30.0 35.0 18.0 36.0 92-93 30.398629678419844 34.0 29.5 35.0 18.0 35.5 94-95 30.220603994826106 34.0 29.0 35.0 18.0 35.0 96-97 30.0370827743605 34.0 29.0 35.0 17.0 35.0 98-99 29.800517405922413 34.0 29.0 35.0 15.5 35.0 100 28.677239970712428 33.0 26.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3786570340922992 1101 2 -0.2773484919718321 1101 3 -0.39587689629355793 1101 4 -0.25359391812134646 1101 5 -0.22759042311547972 1101 6 -0.20198506148016548 1101 7 -0.22806669144608094 1101 8 -0.23117776121943479 1101 9 -0.4561368240523578 1101 10-11 -0.39299524983578493 1101 12-13 -0.39509597492160964 1101 14-15 -0.5452837356580034 1101 16-17 -0.619267664693588 1101 18-19 -0.5662863616262186 1101 20-21 -0.5882400824888521 1101 22-23 -0.6187264664322143 1101 24-25 -0.6460557238475033 1101 26-27 -0.7456223257888794 1101 28-29 -0.663470996866458 1101 30-31 -0.7768413358326143 1101 32-33 -0.7917297869319313 1101 34-35 -0.7805147658201932 1101 36-37 -0.8570614958939942 1101 38-39 -0.8999054687679688 1101 40-41 -0.8073291857260969 1101 42-43 -0.9068566310316442 1101 44-45 -0.8184790491256067 1101 46-47 -1.0119784762951838 1101 48-49 -0.9195483338287644 1101 50-51 -1.057383072976421 1101 52-53 -0.8641469906614532 1101 54-55 -0.7563215252893158 1101 56-57 -0.7279893777965967 1101 58-59 -0.687466237237274 1101 60-61 -0.6954281459299736 1101 62-63 -0.6428568180432634 1101 64-65 -0.612694938974002 1101 66-67 -0.7931322794220748 1101 68-69 -0.6638364684365712 1101 70-71 -0.7088099464544939 1101 72-73 -0.7383121220018083 1101 74-75 -0.7142419340095287 1101 76-77 -0.8531674026373466 1101 78-79 -0.8849704318658169 1101 80-81 -0.8756818290793227 1101 82-83 -0.8760035793808427 1101 84-85 -0.891239923555645 1101 86-87 -0.8792316173371653 1101 88-89 -1.0194321474887253 1101 90-91 -1.0868153327178227 1101 92-93 -1.1937426276118934 1101 94-95 -1.189709845314546 1101 96-97 -1.2726714907531722 1101 98-99 -1.474216922156824 1101 100 -1.3734019697685191 1102 1 -0.3721613080534958 1102 2 -0.277558322533654 1102 3 -0.3693567189800504 1102 4 -0.22717609446299747 1102 5 -0.2801670121058564 1102 6 -0.28062652664991106 1102 7 -0.252471411386324 1102 8 -0.2846084853364559 1102 9 -0.39837895716044613 1102 10-11 -0.3720056225267143 1102 12-13 -0.45430015798964263 1102 14-15 -0.525164504230375 1102 16-17 -0.6641720731735532 1102 18-19 -0.5760242148702375 1102 20-21 -0.6574036437411692 1102 22-23 -0.6153327021198294 1102 24-25 -0.6089015697759592 1102 26-27 -0.6750738160035255 1102 28-29 -0.6349627195297103 1102 30-31 -0.6616064341940344 1102 32-33 -0.6879462991047234 1102 34-35 -0.740978077046698 1102 36-37 -0.7350272598771639 1102 38-39 -0.7537502963945073 1102 40-41 -0.9122227485053642 1102 42-43 -0.8255509080144421 1102 44-45 -1.0247724512798015 1102 46-47 -1.0223322334706637 1102 48-49 -1.0330083072569067 1102 50-51 -1.020098275991387 1102 52-53 -0.9343264035880665 1102 54-55 -0.6540217199617331 1102 56-57 -0.7325457303890133 1102 58-59 -0.7965258944939819 1102 60-61 -0.7201698867195105 1102 62-63 -0.9382761411436746 1102 64-65 -0.9492047996182222 1102 66-67 -0.7848923089943654 1102 68-69 -0.9078304862608064 1102 70-71 -0.9348226879810042 1102 72-73 -1.0047732683204273 1102 74-75 -1.0296064093612856 1102 76-77 -1.0128168853615769 1102 78-79 -1.0362495239989187 1102 80-81 -1.1430757412977535 1102 82-83 -1.1023663726354798 1102 84-85 -1.0802672441853218 1102 86-87 -1.1911624004272703 1102 88-89 -1.1985478698231944 1102 90-91 -1.326814884990192 1102 92-93 -1.2504203794355746 1102 94-95 -1.2786013218166943 1102 96-97 -1.3334223389018867 1102 98-99 -1.392832846804044 1102 100 -1.2903568471763514 1103 1 -0.5656911185946889 1103 2 -0.40946150210239907 1103 3 -0.5293697300354374 1103 4 -0.3802766569621454 1103 5 -0.40053819935094026 1103 6 -0.40476032481856805 1103 7 -0.4303811664134898 1103 8 -0.45790780728979996 1103 9 -0.6760195351625455 1103 10-11 -0.6236717538082175 1103 12-13 -0.6180835715463218 1103 14-15 -0.7819011586120226 1103 16-17 -0.7634444066494197 1103 18-19 -0.6332210261134108 1103 20-21 -0.7351539334893644 1103 22-23 -0.8828756592159905 1103 24-25 -0.8729496085285646 1103 26-27 -0.9087598879550782 1103 28-29 -0.8828679811205902 1103 30-31 -0.9728301720041728 1103 32-33 -0.9863094486778294 1103 34-35 -1.023004328367044 1103 36-37 -0.8679697092790448 1103 38-39 -0.854982150108988 1103 40-41 -0.9511970293339473 1103 42-43 -0.9551697834298309 1103 44-45 -1.0996509906083745 1103 46-47 -1.1204883486878003 1103 48-49 -1.0936024398576691 1103 50-51 -1.1854907470830511 1103 52-53 -1.1391064960682868 1103 54-55 -0.8877897705673803 1103 56-57 -0.7897283127690287 1103 58-59 -0.8624371017446038 1103 60-61 -0.8459551827776508 1103 62-63 -0.8747262040602308 1103 64-65 -0.8805537711229263 1103 66-67 -0.9807392960563348 1103 68-69 -0.9581564098916644 1103 70-71 -0.973522311297792 1103 72-73 -0.9530121993905354 1103 74-75 -0.9045810119837867 1103 76-77 -0.8849438861789345 1103 78-79 -0.9355068863890992 1103 80-81 -1.0192487694798409 1103 82-83 -0.9217583397193536 1103 84-85 -1.02058462064889 1103 86-87 -1.066060810917275 1103 88-89 -1.1292264842323299 1103 90-91 -1.1459264432170926 1103 92-93 -1.2221971743371896 1103 94-95 -1.1586059242998985 1103 96-97 -1.2512451138911125 1103 98-99 -1.2776059652066145 1103 100 -1.4174389220731456 1104 1 -0.4595817481236111 1104 2 -0.45587908087978235 1104 3 -0.5226323489088855 1104 4 -0.3655309667715301 1104 5 -0.3695625054336702 1104 6 -0.331313995575357 1104 7 -0.3843637201394543 1104 8 -0.44264079924298017 1104 9 -0.6248087858285132 1104 10-11 -0.4583738283137464 1104 12-13 -0.4776064904846393 1104 14-15 -0.65457738116244 1104 16-17 -0.7863274586431857 1104 18-19 -0.8383893467715069 1104 20-21 -0.7628122128781953 1104 22-23 -0.6898044800742227 1104 24-25 -0.7131042647789911 1104 26-27 -0.6831378123453504 1104 28-29 -0.8183134546130191 1104 30-31 -0.7588838496621833 1104 32-33 -0.7372237907849097 1104 34-35 -0.9641772590374273 1104 36-37 -0.8930807498443869 1104 38-39 -0.8924324268477406 1104 40-41 -0.8224827948858362 1104 42-43 -0.8111249117234323 1104 44-45 -0.7978376270937204 1104 46-47 -1.0344802880675132 1104 48-49 -1.0118809315279051 1104 50-51 -0.9964460134009769 1104 52-53 -0.761931784884176 1104 54-55 -0.6707612961501823 1104 56-57 -0.7460703922434391 1104 58-59 -0.7419121262439177 1104 60-61 -0.6972546127372112 1104 62-63 -0.8226872397947318 1104 64-65 -0.8503391433054972 1104 66-67 -0.8645482842000476 1104 68-69 -0.942567736167355 1104 70-71 -0.9279429542150766 1104 72-73 -0.8775673470075489 1104 74-75 -0.9862284012537756 1104 76-77 -0.8741904804288438 1104 78-79 -0.955505023060784 1104 80-81 -0.8187324895113086 1104 82-83 -0.8175115384329708 1104 84-85 -0.8790188727641102 1104 86-87 -0.921989990638739 1104 88-89 -1.020019088889267 1104 90-91 -1.1005853851226561 1104 92-93 -1.1470492122662748 1104 94-95 -1.1503803649563409 1104 96-97 -1.157256349070753 1104 98-99 -1.128307266775952 1104 100 -1.1808501931391078 1105 1 -0.5688322819563574 1105 2 -0.4741326601845728 1105 3 -0.5015191763671396 1105 4 -0.2887581169798139 1105 5 -0.24320832053501107 1105 6 -0.3488734172857235 1105 7 -0.41633662593985576 1105 8 -0.4225851406158938 1105 9 -0.6176480759184741 1105 10-11 -0.6002088127363336 1105 12-13 -0.4683461551326573 1105 14-15 -0.7477432718754997 1105 16-17 -0.7716251797899645 1105 18-19 -0.7804387394273675 1105 20-21 -0.7591378683547916 1105 22-23 -0.8356082046515354 1105 24-25 -0.7608117594406636 1105 26-27 -0.7680571248387835 1105 28-29 -0.8617256084968759 1105 30-31 -0.8789367078552033 1105 32-33 -0.8529109472941627 1105 34-35 -0.8516010347226555 1105 36-37 -0.8043012140163412 1105 38-39 -0.8377698791528516 1105 40-41 -0.8625281269123022 1105 42-43 -0.9339586009452603 1105 44-45 -0.9071056636393919 1105 46-47 -0.944335715658795 1105 48-49 -0.9271095169586445 1105 50-51 -0.9171818274521542 1105 52-53 -0.778260765876233 1105 54-55 -0.708872053317549 1105 56-57 -0.6702475897090352 1105 58-59 -0.6642879444957401 1105 60-61 -0.6082930486947831 1105 62-63 -0.6626205544584991 1105 64-65 -0.6204107294013497 1105 66-67 -0.4910556178367642 1105 68-69 -0.5204042258232491 1105 70-71 -0.5706107177184165 1105 72-73 -0.5859392446516765 1105 74-75 -0.7001283557467204 1105 76-77 -0.6342454389474099 1105 78-79 -0.6940304194972313 1105 80-81 -0.7765302981716538 1105 82-83 -0.7906219455460715 1105 84-85 -0.7871571091907583 1105 86-87 -0.7308568650042666 1105 88-89 -0.8178967099205288 1105 90-91 -0.932412668351553 1105 92-93 -1.0131977402881134 1105 94-95 -1.1129543139184292 1105 96-97 -1.0484633361537483 1105 98-99 -1.0560536174603108 1105 100 -0.8820694321211491 1106 1 -0.48562433968853824 1106 2 -0.48868984669554294 1106 3 -0.5787352900853548 1106 4 -0.38639944623879074 1106 5 -0.363216778463638 1106 6 -0.35521174807258404 1106 7 -0.36227784333590307 1106 8 -0.3881187404194506 1106 9 -0.5238008779483749 1106 10-11 -0.5279891862493784 1106 12-13 -0.6162444621827561 1106 14-15 -0.7577186824942928 1106 16-17 -0.788330441440074 1106 18-19 -0.819323725239876 1106 20-21 -0.647426877140127 1106 22-23 -0.6894541748578007 1106 24-25 -0.7671383241574574 1106 26-27 -0.8487936808248193 1106 28-29 -0.8106823502844946 1106 30-31 -0.8152500400062408 1106 32-33 -0.8070614711400665 1106 34-35 -0.9330906191928108 1106 36-37 -0.861844970155154 1106 38-39 -0.7815289168588393 1106 40-41 -0.8846012743530949 1106 42-43 -0.8703677668843639 1106 44-45 -0.8548406098906227 1106 46-47 -0.8136610594326612 1106 48-49 -0.9743431263114744 1106 50-51 -1.0321532137339418 1106 52-53 -0.8932570484801801 1106 54-55 -0.7253433577484145 1106 56-57 -0.6960277313111334 1106 58-59 -0.739664970306066 1106 60-61 -0.709876352470836 1106 62-63 -0.7860525977303539 1106 64-65 -0.7246059302089449 1106 66-67 -0.7881439418027085 1106 68-69 -0.7594701362783454 1106 70-71 -0.7756188004969786 1106 72-73 -0.8387549163749171 1106 74-75 -0.7342143196777471 1106 76-77 -0.8008601530148596 1106 78-79 -0.8432731279442436 1106 80-81 -0.7223052558820093 1106 82-83 -0.6992703480889091 1106 84-85 -0.779215182420046 1106 86-87 -0.7583072769872423 1106 88-89 -0.8396646216306678 1106 90-91 -0.7643759650338637 1106 92-93 -0.9111130996136652 1106 94-95 -0.9481710142349229 1106 96-97 -0.9210937519564375 1106 98-99 -0.8221744976305594 1106 100 -1.0183911198871662 1107 1 -0.6187641049416648 1107 2 -0.5979159357620247 1107 3 -0.6327534652859264 1107 4 -0.5367968432175942 1107 5 -0.4980678094500206 1107 6 -0.4395194396852986 1107 7 -0.4805792390653423 1107 8 -0.4451280732468419 1107 9 -0.6105835563962714 1107 10-11 -0.6518884553435313 1107 12-13 -0.7042071508785526 1107 14-15 -0.7771841487097433 1107 16-17 -0.7954943157444063 1107 18-19 -0.8724083273008176 1107 20-21 -0.8410044921645436 1107 22-23 -0.918948832778888 1107 24-25 -0.9244395256412119 1107 26-27 -0.8702653306353483 1107 28-29 -0.9461373175531307 1107 30-31 -1.0083001263519975 1107 32-33 -1.0272016506979327 1107 34-35 -1.0159950148241563 1107 36-37 -1.1005981290674853 1107 38-39 -1.0226587458968268 1107 40-41 -1.0968020327501833 1107 42-43 -1.1098624796588865 1107 44-45 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1.3304589911791744 2310 94-95 1.3452219231397962 2310 96-97 1.310020162469499 2310 98-99 1.3709523168437627 2310 100 1.5439570258928867 2311 1 0.7574775510677618 2311 2 0.752045447422109 2311 3 0.7123676510911174 2311 4 0.62515097392928 2311 5 0.6107285996876186 2311 6 0.6672304788181762 2311 7 0.6598303499414087 2311 8 0.6425020578998755 2311 9 0.8003556346566612 2311 10-11 0.8150008347590258 2311 12-13 0.9248180378172464 2311 14-15 1.1018606242283937 2311 16-17 1.103342352677565 2311 18-19 1.0683033756004576 2311 20-21 1.1018036900867685 2311 22-23 1.152021665478344 2311 24-25 1.2060008565148124 2311 26-27 1.2063055838810683 2311 28-29 1.1778523135810488 2311 30-31 1.253672754111406 2311 32-33 1.2641549154145508 2311 34-35 1.2667607463534054 2311 36-37 1.3692238323658543 2311 38-39 1.3695325327209886 2311 40-41 1.4061277172435425 2311 42-43 1.4317955734517724 2311 44-45 1.4005464699413182 2311 46-47 1.3692811674580838 2311 48-49 1.452873785453228 2311 50-51 1.387788487946736 2311 52-53 1.307357972528223 2311 54-55 1.2883357388902468 2311 56-57 1.1714444325544093 2311 58-59 1.1911202187695622 2311 60-61 1.2211577861846266 2311 62-63 1.1861159947066255 2311 64-65 1.1762228513036632 2311 66-67 1.2278061632393005 2311 68-69 1.2171532880914029 2311 70-71 1.1967955902593204 2311 72-73 1.1598585290032375 2311 74-75 1.1738187685575454 2311 76-77 1.167359384800271 2311 78-79 1.1915057586103757 2311 80-81 1.1394400172272405 2311 82-83 1.1712890006505887 2311 84-85 1.2466388091765879 2311 86-87 1.1633240255337647 2311 88-89 1.1895402103021055 2311 90-91 1.2924806526131931 2311 92-93 1.2896825936636453 2311 94-95 1.2708972630760016 2311 96-97 1.2533474245141498 2311 98-99 1.4076374771593585 2311 100 1.496273796079219 2312 1 0.6771271601651314 2312 2 0.6357483614235271 2312 3 0.6833662296270084 2312 4 0.3659747123798809 2312 5 0.46642440636850324 2312 6 0.5749490289247845 2312 7 0.4895375283351484 2312 8 0.6164423564073402 2312 9 0.7862461819203475 2312 10-11 0.7635623130816995 2312 12-13 0.9083635246687081 2312 14-15 1.1337195439156673 2312 16-17 1.1107712794721678 2312 18-19 1.0693083507248389 2312 20-21 1.1100002074997022 2312 22-23 1.1721549277384682 2312 24-25 1.1201756966000929 2312 26-27 1.153740196532091 2312 28-29 1.1550928963813405 2312 30-31 1.2149062295911506 2312 32-33 1.1886890305531495 2312 34-35 1.2461061621316603 2312 36-37 1.1930404634959189 2312 38-39 1.227266718932789 2312 40-41 1.304015776945036 2312 42-43 1.309557122847643 2312 44-45 1.2850748992234813 2312 46-47 1.3333796038475754 2312 48-49 1.4404409496323396 2312 50-51 1.5013798170156747 2312 52-53 1.352076878000858 2312 54-55 1.2726392214773128 2312 56-57 1.1869483415807096 2312 58-59 1.1805338648249943 2312 60-61 1.127936748515829 2312 62-63 1.1315339051543845 2312 64-65 1.2009481533647843 2312 66-67 1.2205108540708594 2312 68-69 1.2521458254048312 2312 70-71 1.17667050141781 2312 72-73 1.141127185719661 2312 74-75 1.1312832319548463 2312 76-77 1.132714750827276 2312 78-79 1.1264012809984365 2312 80-81 1.1459759091959647 2312 82-83 1.1956244661800852 2312 84-85 1.2285094559427563 2312 86-87 1.1727941747874944 2312 88-89 1.109077879100969 2312 90-91 1.2089856277375723 2312 92-93 1.2293894166913653 2312 94-95 1.3495767229196431 2312 96-97 1.3118492013442875 2312 98-99 0.9590351317435797 2312 100 1.2570762125682045 2313 1 0.6164648859252289 2313 2 0.6213996465440417 2313 3 0.6504762759317906 2313 4 0.5445852709733998 2313 5 0.3655590364934156 2313 6 0.6225981928206394 2313 7 0.5878024704423481 2313 8 0.6231841547295431 2313 9 0.7373541981948151 2313 10-11 0.7851282145312197 2313 12-13 0.8501495064477567 2313 14-15 1.0959520216427663 2313 16-17 1.102812328601658 2313 18-19 1.0191127308111803 2313 20-21 1.045483915469653 2313 22-23 1.071528766860176 2313 24-25 1.0153800217483848 2313 26-27 1.0013178342705302 2313 28-29 1.0568339936344628 2313 30-31 1.1039101850701911 2313 32-33 1.1313001902643265 2313 34-35 1.2035317217312382 2313 36-37 1.2418564016093896 2313 38-39 1.2399566329091485 2313 40-41 1.2964232743277293 2313 42-43 1.3163797177453773 2313 44-45 1.2105657205533262 2313 46-47 1.2329989027040043 2313 48-49 1.2681068969105311 2313 50-51 1.1957214598042185 2313 52-53 1.1918884821687001 2313 54-55 1.1413839716375307 2313 56-57 1.1279065686338825 2313 58-59 1.0539523752832096 2313 60-61 0.9929245937998914 2313 62-63 1.0654791565412438 2313 64-65 1.0422110460150549 2313 66-67 1.0573803939229336 2313 68-69 0.949101504653207 2313 70-71 1.0092528302293786 2313 72-73 1.0069693186526223 2313 74-75 1.0111020853682007 2313 76-77 0.8075652439460796 2313 78-79 0.9491418279651782 2313 80-81 0.9514973138464917 2313 82-83 1.0220191683726796 2313 84-85 1.0949304716282882 2313 86-87 1.094387907622501 2313 88-89 1.1108880465049928 2313 90-91 1.141690042218066 2313 92-93 1.211557631598378 2313 94-95 1.2708248734913639 2313 96-97 1.30005129082204 2313 98-99 1.3765574298168097 2313 100 1.406193253056177 2314 1 0.540460698236096 2314 2 0.52720319776504 2314 3 0.584198926811279 2314 4 0.422844683511876 2314 5 0.47734745134351186 2314 6 0.45685226992144834 2314 7 0.4899777142369146 2314 8 0.45751009238939133 2314 9 0.5893605337356362 2314 10-11 0.644474477714148 2314 12-13 0.7458461585305542 2314 14-15 0.8742571556804819 2314 16-17 0.8680439987681083 2314 18-19 0.8276128014284083 2314 20-21 0.9181455478175096 2314 22-23 0.9613000311455977 2314 24-25 0.9279636235146143 2314 26-27 0.9616601792171409 2314 28-29 1.0045518040768329 2314 30-31 1.023643261655991 2314 32-33 0.9518906590947438 2314 34-35 1.0264474012622884 2314 36-37 1.032400395172047 2314 38-39 1.0405060776945376 2314 40-41 1.05126322576794 2314 42-43 1.1127488362383744 2314 44-45 1.1443664777798404 2314 46-47 1.155193082018151 2314 48-49 1.1556570501994585 2314 50-51 1.2411329911801232 2314 52-53 1.1698830558125692 2314 54-55 1.0519032481984922 2314 56-57 1.07052458148641 2314 58-59 1.0281072852010738 2314 60-61 1.0769194949833647 2314 62-63 1.1000170333113601 2314 64-65 1.0738647286307312 2314 66-67 1.0832107291809052 2314 68-69 1.0603632626161925 2314 70-71 1.0230421119132487 2314 72-73 1.0600750682938482 2314 74-75 1.1168468892708532 2314 76-77 1.0695139997326635 2314 78-79 1.0406811456376133 2314 80-81 1.0017139737234153 2314 82-83 1.070379731593171 2314 84-85 1.1379807648889155 2314 86-87 1.173045855829674 2314 88-89 1.263466430172766 2314 90-91 1.2767844934911068 2314 92-93 1.3056550608398183 2314 94-95 1.2380750016611515 2314 96-97 1.2670154629229309 2314 98-99 1.3717230150782278 2314 100 1.4370774409939777 2315 1 0.3991877260667849 2315 2 0.4341747979290922 2315 3 0.5048765576408556 2315 4 0.20888471702915012 2315 5 0.2812033285761615 2315 6 0.30232189329571924 2315 7 0.360715210764333 2315 8 0.34746881983073763 2315 9 0.5065198283692283 2315 10-11 0.41768939914065584 2315 12-13 0.49619087969173137 2315 14-15 0.6604343822051391 2315 16-17 0.6557039585969946 2315 18-19 0.5131526885237676 2315 20-21 0.6223295680180954 2315 22-23 0.4077589516232649 2315 24-25 0.6511347875330884 2315 26-27 0.6546156688358522 2315 28-29 0.7711204300904697 2315 30-31 0.7753604767751483 2315 32-33 0.802431762300202 2315 34-35 0.7737161157305366 2315 36-37 0.850695859927221 2315 38-39 0.8893723322746823 2315 40-41 0.8862532506577878 2315 42-43 1.01648079053669 2315 44-45 1.0191814580006664 2315 46-47 1.0196330642595584 2315 48-49 0.959135500991728 2315 50-51 0.8852878811268567 2315 52-53 0.8746819164658959 2315 54-55 0.7447831021600209 2315 56-57 0.6816090960629992 2315 58-59 0.7766757525753007 2315 60-61 0.7852697542384917 2315 62-63 0.7324044886188474 2315 64-65 0.7337488466840014 2315 66-67 0.7671844469178026 2315 68-69 0.8243740726507767 2315 70-71 0.7318077658064368 2315 72-73 0.4857425285334429 2315 74-75 0.6722477902073152 2315 76-77 0.7198823852309104 2315 78-79 0.7597495827500609 2315 80-81 0.4845190604093226 2315 82-83 0.40770837190689946 2315 84-85 0.747453435493 2315 86-87 0.8480302268413666 2315 88-89 0.8835105935773662 2315 90-91 0.951539983936847 2315 92-93 0.9134604388658545 2315 94-95 0.9807687738617368 2315 96-97 0.850246300918645 2315 98-99 0.22065507493565661 2315 100 0.7959528079415712 2316 1 0.3461716405084019 2316 2 0.3582479790676736 2316 3 0.3439186395707061 2316 4 0.2777244546371591 2316 5 0.21328633380387885 2316 6 0.24666766515623095 2316 7 0.17154252256468538 2316 8 0.22640912253201861 2316 9 0.3984739271165836 2316 10-11 0.3594612041435923 2316 12-13 0.13370731388039303 2316 14-15 0.19644563550497907 2316 16-17 0.3354618947057304 2316 18-19 0.35634884769343245 2316 20-21 0.3836028143730914 2316 22-23 0.3594632507029587 2316 24-25 0.4374018764875842 2316 26-27 0.4581668411702182 2316 28-29 0.45702622706622975 2316 30-31 0.2733614897108936 2316 32-33 0.455162179164887 2316 34-35 0.515355926880467 2316 36-37 0.4032427689121718 2316 38-39 0.5704588793085534 2316 40-41 0.6312284649526703 2316 42-43 0.5519578243000538 2316 44-45 0.48097348702890486 2316 46-47 0.31867632563513837 2316 48-49 0.20004076027900197 2316 50-51 0.3065668165730244 2316 52-53 0.3550875070386539 2316 54-55 0.13543460716746125 2316 56-57 -0.03757614976470336 2316 58-59 0.04891437526021036 2316 60-61 0.09613956077470931 2316 62-63 0.2711718290727987 2316 64-65 0.2446248973446643 2316 66-67 0.16688819790211795 2316 68-69 0.14965566531878238 2316 70-71 -0.09991481468205876 2316 72-73 -0.21532088055037235 2316 74-75 0.009878006219331326 2316 76-77 0.20703683364924785 2316 78-79 0.23331525532898922 2316 80-81 0.34560203441641235 2316 82-83 0.41317371528171165 2316 84-85 0.4847527129090423 2316 86-87 0.48860714925118387 2316 88-89 0.49975364265271693 2316 90-91 0.36078423674062776 2316 92-93 0.3563362397615393 2316 94-95 0.4826099233356693 2316 96-97 0.4606146695717541 2316 98-99 0.2807669141425144 2316 100 0.32737116081091955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 29.0 1 366.0 2 135.0 3 136.0 4 116.0 5 91.0 6 88.0 7 452.0 8 1510.0 9 3089.0 10 4816.0 11 7214.0 12 10603.0 13 13149.0 14 15658.0 15 18398.0 16 22112.0 17 27876.0 18 34654.0 19 41012.0 20 48496.0 21 57783.0 22 63024.0 23 64858.0 24 63187.0 25 65014.0 26 70415.0 27 80367.0 28 93886.0 29 111393.0 30 134450.0 31 166746.0 32 211149.0 33 275651.0 34 387315.0 35 536813.0 36 799908.0 37 1160883.0 38 1069724.0 39 287698.0 40 383.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.24630623044495 18.763434640725087 12.38418297207209 23.606076156757876 2 16.046433441608706 19.77774382487121 40.937058715045524 23.23876401847457 3 19.450069843845935 25.97969208872849 28.99036438973992 25.57987367768565 4 12.669644167225655 16.049678195643402 38.191612968178575 33.089064668952375 5 13.212823905434606 37.20673540433806 34.413712842730305 15.166727847497032 6 30.442077504839364 36.402857836056604 18.77919361111256 14.375871047991476 7 27.48604452871038 32.054245037545556 22.508835508630877 17.95087492511319 8 26.17743090699387 35.037963744455915 20.403293329612058 18.38131201893816 9 26.789627784588344 16.055979917542494 21.030379222483962 36.1240130753852 10-11 25.484679135406207 25.854681367120335 27.958284177483055 20.7023553199904 12-13 25.645570209729463 23.68170225313983 28.194193867214352 22.478533669916356 14-15 22.927475520428057 25.043636008832 26.37730236726556 25.651586103474383 16-17 22.14329006846564 27.56145250551554 27.251404054824953 23.043853371193872 18-19 21.697849037468092 27.0562623948054 29.3829267296505 21.862961838076 20-21 22.795475173967123 26.44305308098296 29.116289037550004 21.645182707499917 22-23 22.724576003735667 26.230444962745448 28.878710757868554 22.16626827565033 24-25 22.197786607918097 26.16366803942954 29.415234280876174 22.223311071776187 26-27 22.131197621039984 26.427758813969028 28.922972479744274 22.51807108524671 28-29 21.85911847737735 26.9521813845948 29.00711048480517 22.181589653222677 30-31 22.649655036560343 26.49202906540523 29.046967064765187 21.81134883326924 32-33 22.543489014854114 26.58713409951253 28.65422276817238 22.215154117460976 34-35 22.635622704126998 26.71891908609841 28.855562252931538 21.78989595684305 36-37 22.450734909678392 26.633786994148945 28.60474359158609 22.31073450458657 38-39 22.476126572155128 26.838829212439514 28.70421994027294 21.98082427513242 40-41 22.53444420799753 26.17810234879192 28.996218978529164 22.291234464681388 42-43 22.3679770197699 26.448848925041673 28.493432797107232 22.689741258081195 44-45 22.235962150201686 26.979636092996607 28.61546833825485 22.168933418546853 46-47 22.23252283276317 26.623903441023188 28.196357770242862 22.947215955970773 48-49 22.525094012447124 26.683252969786313 28.228010056349888 22.563642961416672 50-51 22.46549561485935 26.840651314499997 28.093737151102534 22.600115919538126 52-53 22.271620118465847 27.158901712448262 27.835792987118246 22.733685181967644 54-55 22.11545670856744 27.00634681225412 28.054829975566943 22.8233665036115 56-57 22.592462149358315 26.905392728776572 27.660186470938307 22.84195865092681 58-59 22.170294036800204 27.0559921587945 28.11433634299558 22.65937746140972 60-61 22.487576112855034 27.297496732767712 27.79485563245633 22.420071521920924 62-63 22.557479161865494 27.024606707368832 27.683407269271882 22.734506861493788 64-65 22.17332980003498 26.86684264240625 27.847694333720703 23.112133223838065 66-67 22.23650069060849 26.75239014920724 27.766890784173686 23.24421837601058 68-69 21.973276533077353 26.90300470888468 27.93222635596882 23.19149240206915 70-71 22.08913495659001 26.679830251953206 27.929681573208153 23.30135321824863 72-73 22.297591568586064 26.993691957260218 27.75377407840883 22.954942395744883 74-75 22.4811795564989 27.07927543719379 27.512949757772233 22.92659524853508 76-77 22.404740037825636 26.912048669076764 27.43100821527669 23.252203077820905 78-79 22.24689684662334 26.989534463121856 27.565228075554103 23.198340614700697 80-81 22.155694661947013 27.197647198635572 27.607856129689495 23.03880200972792 82-83 22.667865776965147 26.879719689898714 27.98870341954859 22.463711113587546 84-85 22.80046928610511 27.14270191785947 27.301217493403446 22.755611302631973 86-87 22.191838059396577 27.410236788617716 27.588059774742984 22.809865377242726 88-89 22.523535700452076 26.783455252019678 27.583384590922417 23.109624456605825 90-91 22.710862277999567 26.784924586119974 27.57804231003321 22.926170825847244 92-93 22.78426949313803 26.907477795526475 27.561701177450814 22.746551533884688 94-95 22.23492883981316 26.971055105809278 27.701814952669835 23.09220110170773 96-97 22.407234195158978 26.899366622153188 27.504454639578245 23.188944543109592 98-99 22.19663043454508 27.11132388416995 27.305240229505777 23.386805451779193 100 22.607973262762915 26.90706803004342 26.990008332693154 23.494950374500515 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1399.0 1 1040.5 2 1981.0 3 2949.5 4 1973.5 5 1371.5 6 1862.5 7 3192.0 8 4850.5 9 5651.5 10 5166.0 11 4625.5 12 4831.0 13 5398.5 14 6014.0 15 6689.0 16 7428.0 17 8146.0 18 8944.0 19 9628.0 20 10392.5 21 11486.0 22 13117.0 23 15388.0 24 18144.0 25 21702.5 26 26585.5 27 32177.0 28 38966.5 29 48604.5 30 60894.5 31 73225.5 32 88481.0 33 103575.0 34 115787.5 35 128383.0 36 141450.0 37 158948.5 38 169065.5 39 169976.0 40 169548.0 41 167942.0 42 170432.0 43 177973.0 44 188745.0 45 206845.0 46 224168.0 47 236478.5 48 247167.0 49 251755.5 50 250489.0 51 249939.0 52 245037.0 53 234251.0 54 221766.0 55 199799.0 56 178089.5 57 154960.5 58 130696.5 59 113826.5 60 93238.0 61 71789.0 62 56651.0 63 45105.0 64 33556.5 65 22729.0 66 16089.0 67 12112.0 68 9374.5 69 7190.0 70 5672.5 71 4465.0 72 3416.5 73 2668.5 74 2147.5 75 1600.0 76 1185.5 77 875.0 78 579.5 79 389.0 80 281.5 81 190.5 82 131.0 83 100.0 84 79.5 85 63.5 86 55.0 87 41.5 88 34.5 89 31.5 90 23.5 91 19.5 92 17.5 93 12.0 94 7.5 95 6.5 96 5.5 97 6.0 98 4.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05604432593632255 2 0.13065806121586443 3 0.015897430985235723 4 0.015359674334572358 5 0.017998042901889492 6 0.05159102867301656 7 0.056094740622322246 8 0.04817963492037084 9 0.039844406835088685 10-11 0.03872688129542888 12-13 0.04094512747941526 14-15 0.01925000760421514 16-17 0.018367750599220557 18-19 0.018115677169222102 20-21 0.020955704480537997 22-23 0.025350184610177684 24-25 0.024409110471516796 26-27 0.019434861452880668 28-29 0.018871897459217458 30-31 0.017325847088560286 32-33 0.023291584931856988 34-35 0.02327478003652376 36-37 0.023165548216857762 38-39 0.02100611916653769 40-41 0.02455195208184925 42-43 0.031618410569472524 44-45 0.041197200909413714 46-47 0.046709206578713205 48-49 0.03975197991075592 50-51 0.028450687799158644 52-53 0.03949990648075747 54-55 0.0361137200711116 56-57 0.038096697720432754 58-59 0.043390239750400246 60-61 0.023896561163853275 62-63 0.02651812483583718 64-65 0.025736697202841975 66-67 0.032055337848136516 68-69 0.03893694248709426 70-71 0.04065944425875035 72-73 0.04378515479073116 74-75 0.026845820294835166 76-77 0.020888484899205077 78-79 0.020417947829874633 80-81 0.024930062226846926 82-83 0.020770850631872468 84-85 0.01274651311025507 86-87 0.015292454753239437 88-89 0.018846690116217615 90-91 0.02147665623586813 92-93 0.020611204126206782 94-95 0.02400579298351927 96-97 0.0254174041915106 98-99 0.028400273113158958 100 0.03008076264648197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5950647.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.15882566843604 #Duplication Level Percentage of deduplicated Percentage of total 1 80.19860745911186 54.662429046569386 2 12.761912779439255 17.396739766591722 3 3.495425360559358 7.147322633621863 4 1.3673918291142066 3.727992852041564 5 0.6610859873304289 2.2529422281150313 6 0.3991891164196046 1.632495683686851 7 0.24429487963545057 1.1655596478934969 8 0.17179326660493252 0.93673818476294 9 0.12200522165686856 0.7484159370194462 >10 0.543943641398732 6.210897255453475 >50 0.022424700945256486 1.0471279831083264 >100 0.010940440156987545 1.349821538106546 >500 5.795990471197395E-4 0.2809184771160042 >1k 2.8979898574410816E-4 0.3935262393431367 >5k 2.8979898572448537E-5 0.10040123849381535 >10k+ 8.69396957173456E-5 0.946671288076547 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21168 0.35572602441381584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16300 0.2739197939316515 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10459 0.17576240029025414 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.680489533323015E-5 0.0 5 0.0 0.0 0.0 3.36097906664603E-5 0.0 6 0.0 0.0 0.0 3.36097906664603E-5 0.0 7 0.0 0.0 0.0 3.36097906664603E-5 0.0 8 0.0 0.0 0.0 3.36097906664603E-5 0.0 9 0.0 0.0 0.0 3.36097906664603E-5 1.680489533323015E-5 10-11 0.0 0.0 0.0 4.201223833307538E-5 1.680489533323015E-5 12-13 0.0 0.0 0.0 5.041468599969045E-5 1.680489533323015E-5 14-15 0.0 0.0 0.0 6.72195813329206E-5 4.201223833307538E-5 16-17 0.0 0.0 0.0 6.72195813329206E-5 5.041468599969045E-5 18-19 0.0 0.0 0.0 8.402447666615076E-5 5.8817133666305525E-5 20-21 0.0 0.0 0.0 1.6804895333230152E-4 9.242692433276583E-5 22-23 1.680489533323015E-5 0.0 0.0 3.781101449976784E-4 1.5964650566568643E-4 24-25 1.680489533323015E-5 0.0 0.0 9.494765863275036E-4 1.8485384866553166E-4 26-27 1.680489533323015E-5 0.0 0.0 0.0018317335913220867 1.8485384866553166E-4 28-29 3.36097906664603E-5 0.0 0.0 0.004865017198970129 1.8485384866553166E-4 30-31 3.36097906664603E-5 0.0 0.0 0.017594725413891968 1.8485384866553166E-4 32-33 3.36097906664603E-5 0.0 0.0 0.04730578036304288 2.3526853466522213E-4 34-35 3.36097906664603E-5 0.0 0.0 0.09070442256110975 2.520734299984523E-4 36-37 3.36097906664603E-5 0.0 0.0 0.15836933362036093 2.520734299984523E-4 38-39 3.36097906664603E-5 0.0 0.0 0.26140014690839497 2.520734299984523E-4 40-41 3.36097906664603E-5 0.0 0.0 0.37870671878200807 2.520734299984523E-4 42-43 3.36097906664603E-5 0.0 0.0 0.4914171517819827 2.520734299984523E-4 44-45 3.36097906664603E-5 0.0 0.0 0.6199493937381935 2.520734299984523E-4 46-47 3.36097906664603E-5 0.0 0.0 0.764295042202974 2.520734299984523E-4 48-49 5.041468599969045E-5 0.0 0.0 0.9233701814273305 2.520734299984523E-4 50-51 5.041468599969045E-5 0.0 0.0 1.0903436214583053 2.520734299984523E-4 52-53 5.041468599969045E-5 0.0 0.0 1.254678692921963 2.688783253316824E-4 54-55 5.041468599969045E-5 0.0 0.0 1.4405576402028215 2.688783253316824E-4 56-57 5.041468599969045E-5 0.0 0.0 1.6484846101608783 2.772807729982975E-4 58-59 5.8817133666305525E-5 0.0 0.0 1.8876182707527434 2.8568322066491257E-4 60-61 6.72195813329206E-5 0.0 0.0 2.1248529781719534 2.8568322066491257E-4 62-63 6.72195813329206E-5 0.0 0.0 2.368683607009456 3.0248811599814274E-4 64-65 6.72195813329206E-5 0.0 0.0 2.615169409309609 3.0248811599814274E-4 66-67 6.72195813329206E-5 0.0 0.0 2.890836912355917 3.108905636647578E-4 68-69 6.72195813329206E-5 0.0 0.0 3.200290657469684 3.1929301133137286E-4 70-71 6.72195813329206E-5 0.0 0.0 3.523095891925702 3.5290280199783315E-4 72-73 6.72195813329206E-5 0.0 0.0 3.8378935937554353 3.865125926642935E-4 74-75 6.72195813329206E-5 0.0 0.0 4.15797643516747 3.9491504033090856E-4 76-77 6.72195813329206E-5 0.0 0.0 4.5016449471796935 4.033174879975236E-4 78-79 6.72195813329206E-5 0.0 0.0 4.874436342804405 4.033174879975236E-4 80-81 6.72195813329206E-5 0.0 0.0 5.258302164453714 4.2012238333075374E-4 82-83 6.72195813329206E-5 0.0 0.0 5.6523769600179605 5.041468599969045E-4 84-85 6.72195813329206E-5 0.0 0.0 6.043006752038896 5.041468599969045E-4 86-87 6.72195813329206E-5 0.0 0.0 6.457297836689019 5.041468599969045E-4 88 6.72195813329206E-5 0.0 0.0 6.794538476236282 5.041468599969045E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9450 0.0 48.653046 1 GTATCAA 17105 0.0 41.48829 1 TACCTGG 5325 0.0 40.475246 2 GTACCTG 6405 0.0 34.899704 1 ATCAACG 20495 0.0 33.78525 3 CAACGCA 20630 0.0 33.51859 5 TATCAAC 21715 0.0 32.355164 2 ACCTGGG 6615 0.0 32.19146 3 AACGCAG 22545 0.0 31.214388 6 GTACTAG 3060 0.0 31.065418 1 TCAACGC 22455 0.0 31.003756 4 GTACATG 20750 0.0 29.369661 1 GTGGTAT 4105 0.0 28.774502 1 TACATGG 21125 0.0 28.157492 2 GCATCGT 2895 0.0 27.765251 8 ACGCAGA 25055 0.0 27.749855 7 CGCAGAG 25585 0.0 27.15458 8 ACATGGG 21230 0.0 27.080008 3 CATGGGG 16470 0.0 25.858715 4 ATGGGGG 12840 0.0 24.7854 5 >>END_MODULE