FastQCFastQC Report
Mon 6 Jun 2016
ERR1211188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5950647
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194520.32688882402199293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156050.2622403916750565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104390.17542630238358956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98900.048.5319981
TACCTGG54350.044.356362
GTATCAA173750.040.233231
GTACCTG65150.037.6664621
ACCTGGG68300.034.6121983
ATCAACG210250.032.2114223
CAACGCA214350.031.7177855
TATCAAC224000.030.9653822
GTGGTAT39450.030.5064491
TCAACGC228150.029.8490034
AACGCAG234150.029.5975446
GTACATG208450.029.3860531
GTACTAG30200.029.1094111
CCTGGGG65150.028.3504564
TACATGG210350.028.2609082
ACATGGG210350.026.9902113
ACGCAGA257600.026.4836927
CTGTGCG41500.026.2662249
CGCAGAG261550.026.0116548
TGGGGGG129350.025.462996
Produced by FastQC (version 0.11.5)