##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211188_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5950647 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15353674986938 33.0 31.0 34.0 30.0 34.0 2 32.28033775150836 34.0 31.0 34.0 30.0 34.0 3 32.362022650646225 34.0 31.0 34.0 30.0 34.0 4 35.98087905399194 37.0 35.0 37.0 35.0 37.0 5 35.86907541314415 37.0 35.0 37.0 35.0 37.0 6 35.897438715487574 37.0 35.0 37.0 35.0 37.0 7 35.83864981404543 37.0 35.0 37.0 35.0 37.0 8 35.82476544147216 37.0 35.0 37.0 35.0 37.0 9 37.435950410098265 39.0 37.0 39.0 34.0 39.0 10-11 37.41143442049243 39.0 37.0 39.0 34.0 39.0 12-13 37.312453755028656 39.0 37.0 39.0 34.0 39.0 14-15 38.53986927808018 40.0 38.0 41.0 34.0 41.0 16-17 38.48906244984789 40.0 38.0 41.0 34.0 41.0 18-19 38.515939359199095 40.0 38.0 41.0 34.0 41.0 20-21 38.47452823197209 40.0 38.0 41.0 34.0 41.0 22-23 38.34565359027346 40.0 38.0 41.0 34.0 41.0 24-25 38.28157391960907 40.0 38.0 41.0 34.0 41.0 26-27 38.14142882278179 40.0 38.0 41.0 33.5 41.0 28-29 38.10709348075932 40.0 37.5 41.0 33.5 41.0 30-31 38.10799371900232 40.0 37.5 41.0 33.5 41.0 32-33 37.963040237473336 40.0 37.0 41.0 33.0 41.0 34-35 37.80842948674321 40.0 37.0 41.0 33.0 41.0 36-37 37.652739189536874 40.0 37.0 41.0 33.0 41.0 38-39 37.559215745783604 40.0 37.0 41.0 32.5 41.0 40-41 37.45848930376815 40.0 36.0 41.0 32.0 41.0 42-43 37.30872752156194 40.0 36.0 41.0 32.0 41.0 44-45 37.16954248840504 40.0 36.0 41.0 31.0 41.0 46-47 36.93215771327051 39.0 36.0 41.0 31.0 41.0 48-49 36.70376658202041 39.0 35.0 41.0 30.0 41.0 50-51 36.508220030527774 39.0 35.0 41.0 30.0 41.0 52-53 36.31231948391495 39.0 35.0 40.0 29.5 41.0 54-55 36.07695264061202 38.5 35.0 40.0 29.0 41.0 56-57 35.8318606363308 38.0 35.0 40.0 28.5 41.0 58-59 35.571658510410714 38.0 34.0 40.0 28.0 41.0 60-61 35.30891464407148 37.5 34.0 40.0 28.0 41.0 62-63 35.00322998490752 37.0 34.0 40.0 27.0 41.0 64-65 34.632832362598556 36.5 34.0 39.5 26.0 41.0 66-67 34.326805219667705 36.0 33.0 39.0 26.0 40.5 68-69 33.99509339068508 35.5 33.0 39.0 26.0 40.0 70-71 33.66283918370557 35.0 33.0 38.0 26.0 40.0 72-73 33.31241283510852 35.0 32.5 37.5 25.5 40.0 74-75 32.971035082403645 35.0 32.0 37.0 25.0 39.0 76-77 31.97275329892699 34.0 30.5 36.0 24.5 38.5 78-79 32.4619503559865 35.0 32.0 36.0 25.0 38.5 80-81 32.38512543257902 35.0 32.0 36.0 25.0 37.5 82-83 32.12500338198518 35.0 32.0 35.0 25.0 37.0 84-85 31.866364447428992 34.0 31.0 35.0 24.0 36.5 86-87 31.623056786934264 34.0 31.0 35.0 24.0 36.0 88-89 31.4023896057017 34.0 31.0 35.0 23.5 36.0 90-91 31.184258703297303 34.0 31.0 35.0 23.0 35.5 92-93 30.99482678102062 34.0 31.0 35.0 21.5 35.0 94-95 30.80682293874935 34.0 30.0 35.0 20.0 35.0 96-97 30.63092509100271 34.0 30.0 35.0 20.0 35.0 98-99 30.451167999042795 34.0 30.0 35.0 19.5 35.0 100 28.68021040401153 32.0 26.0 34.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.31120803138203 1101 2 -0.33865890219751904 1101 3 -0.38049544186038986 1101 4 -0.20235014186526 1101 5 -0.22315596512319047 1101 6 -0.31197147273622505 1101 7 -0.26727127436380727 1101 8 -0.351903879287768 1101 9 -0.4217591224170434 1101 10-11 -0.4526712291114592 1101 12-13 -0.43416088094843275 1101 14-15 -0.6227257075738564 1101 16-17 -0.5957317700202864 1101 18-19 -0.6466337856408941 1101 20-21 -0.6376510136744145 1101 22-23 -0.6446508230509735 1101 24-25 -0.6814047905782559 1101 26-27 -0.7985875637254267 1101 28-29 -0.8591685298517575 1101 30-31 -0.6787781577398633 1101 32-33 -0.5935898583951413 1101 34-35 -0.616586815137488 1101 36-37 -0.58909911188411 1101 38-39 -0.7212587582023176 1101 40-41 -0.7736034356459598 1101 42-43 -0.7688491123231813 1101 44-45 -0.7015058191433283 1101 46-47 -0.7130415388104652 1101 48-49 -0.7039117330402007 1101 50-51 -0.8106152926753438 1101 52-53 -0.6280199181239396 1101 54-55 -0.6077897659508764 1101 56-57 -0.6146106400480207 1101 58-59 -0.7271314048312476 1101 60-61 -0.768717333968759 1101 62-63 -0.8694174554602441 1101 64-65 -0.8793109318902523 1101 66-67 -0.9058005012099599 1101 68-69 -1.039656044402406 1101 70-71 -0.9502593339658105 1101 72-73 -0.971991354605457 1101 74-75 -1.0638991848815742 1101 76-77 -0.9534733984501287 1101 78-79 -0.9032574748034179 1101 80-81 -0.8229515086073995 1101 82-83 -0.7549317609687378 1101 84-85 -0.6973169251569011 1101 86-87 -0.8251889032837312 1101 88-89 -0.7987526915559862 1101 90-91 -0.8278692358933419 1101 92-93 -0.9457836103802997 1101 94-95 -0.8865257281404872 1101 96-97 -0.8805437959435842 1101 98-99 -1.0927444786770621 1101 100 -0.8842264889034475 1102 1 -0.34650021765498806 1102 2 -0.3316014004932022 1102 3 -0.41702545024804394 1102 4 -0.23457606210982362 1102 5 -0.3333987593534715 1102 6 -0.32214881185971933 1102 7 -0.3195079467540367 1102 8 -0.3597434564725077 1102 9 -0.48894210146082884 1102 10-11 -0.5626434376995277 1102 12-13 -0.4938434754699159 1102 14-15 -0.591694117493681 1102 16-17 -0.6637823605696909 1102 18-19 -0.7275049789675663 1102 20-21 -0.7795578411087121 1102 22-23 -0.7013744057006619 1102 24-25 -0.7206237820706463 1102 26-27 -0.8485439436938336 1102 28-29 -0.8331021501094469 1102 30-31 -0.7299869499522558 1102 32-33 -0.7224917573728575 1102 34-35 -0.7333432667868607 1102 36-37 -0.785712074623703 1102 38-39 -0.8591757421486079 1102 40-41 -0.8193425999968582 1102 42-43 -0.8135217493598574 1102 44-45 -0.846698432795705 1102 46-47 -0.8471507774474034 1102 48-49 -0.8383264177046215 1102 50-51 -0.8851180059382031 1102 52-53 -0.9628831545955308 1102 54-55 -0.8985749409984578 1102 56-57 -1.0134766528764203 1102 58-59 -1.003247374306909 1102 60-61 -0.951941569888703 1102 62-63 -0.9247560338893734 1102 64-65 -0.9598772917521146 1102 66-67 -0.984898882451823 1102 68-69 -0.8675823983302067 1102 70-71 -0.9502532083390065 1102 72-73 -0.9415793438328208 1102 74-75 -1.0667883089202839 1102 76-77 -0.9104868680352212 1102 78-79 -0.8299667446476349 1102 80-81 -0.7847653534818022 1102 82-83 -0.8313135380977172 1102 84-85 -0.9302461675961808 1102 86-87 -0.8431321450077576 1102 88-89 -0.9561270607974102 1102 90-91 -0.8721350756226656 1102 92-93 -0.9675458462920012 1102 94-95 -1.1427682151984264 1102 96-97 -1.2071804269914033 1102 98-99 -1.2276737716806068 1102 100 -1.2230196490953809 1103 1 -0.38288699115104663 1103 2 -0.33994324300329026 1103 3 -0.3806366716958678 1103 4 -0.32450240690096166 1103 5 -0.3140149672703316 1103 6 -0.38257152975057807 1103 7 -0.43986147543471077 1103 8 -0.3608095835047038 1103 9 -0.559394215584966 1103 10-11 -0.5548156458488265 1103 12-13 -0.5598586290508791 1103 14-15 -0.6980412914831575 1103 16-17 -0.7400072599053189 1103 18-19 -0.6563058055572313 1103 20-21 -0.6989371024685482 1103 22-23 -0.7553605592755801 1103 24-25 -0.8199143721101194 1103 26-27 -0.7192732093381622 1103 28-29 -0.7951649933521949 1103 30-31 -0.7240242824087133 1103 32-33 -0.7185512416893047 1103 34-35 -0.8589344065775393 1103 36-37 -0.7548031847958399 1103 38-39 -0.7697654706611985 1103 40-41 -0.8540106710470425 1103 42-43 -0.6611479669389766 1103 44-45 -0.7515828972410006 1103 46-47 -0.8130998415645863 1103 48-49 -0.8342600056026939 1103 50-51 -0.9756356839903546 1103 52-53 -0.9214878564450899 1103 54-55 -0.8679939833752144 1103 56-57 -0.8980137023033734 1103 58-59 -0.8407340582719414 1103 60-61 -0.9521254898170355 1103 62-63 -0.8579407046232745 1103 64-65 -0.8901326106735254 1103 66-67 -0.9096229726593705 1103 68-69 -0.8359930733615428 1103 70-71 -1.0271395651351511 1103 72-73 -0.9475699062799023 1103 74-75 -0.9658783722420665 1103 76-77 -0.9182303595878842 1103 78-79 -0.7826308873355927 1103 80-81 -0.7561176217208825 1103 82-83 -0.8033878551861555 1103 84-85 -0.8522003958936999 1103 86-87 -0.884420841007902 1103 88-89 -0.9697510477691864 1103 90-91 -0.8438626411201113 1103 92-93 -0.9500479677820799 1103 94-95 -0.992315434832836 1103 96-97 -0.9568591607189632 1103 98-99 -1.0517255419481728 1103 100 -1.1509569472618608 1104 1 -0.37191668018225243 1104 2 -0.3408880194729029 1104 3 -0.37537408042728515 1104 4 -0.24754473895987417 1104 5 -0.2931048349050087 1104 6 -0.364335480558708 1104 7 -0.36242679544409384 1104 8 -0.380637605202125 1104 9 -0.5331305339242576 1104 10-11 -0.5542563022924938 1104 12-13 -0.5593074979415817 1104 14-15 -0.6974934729575395 1104 16-17 -0.6361679064112309 1104 18-19 -0.6628651353111294 1104 20-21 -0.7474734235683229 1104 22-23 -0.7029349198779116 1104 24-25 -0.7263371267994927 1104 26-27 -0.8440075125223743 1104 28-29 -0.8858113612422116 1104 30-31 -0.8674983545402242 1104 32-33 -0.8462539649091383 1104 34-35 -0.8848572371802916 1104 36-37 -0.7207888854763027 1104 38-39 -0.8568155458844515 1104 40-41 -0.8379215901509909 1104 42-43 -0.6556532187643498 1104 44-45 -0.8088319345213719 1104 46-47 -0.9150733823024737 1104 48-49 -0.8790827133638004 1104 50-51 -0.8391363948156041 1104 52-53 -0.8419090376572171 1104 54-55 -0.8375828779384378 1104 56-57 -0.7833981000342618 1104 58-59 -0.7928725380720749 1104 60-61 -0.8098373916541348 1104 62-63 -0.8802971938366824 1104 64-65 -0.8696765093084622 1104 66-67 -0.9040831595442995 1104 68-69 -0.9427200336519519 1104 70-71 -0.9003558768492752 1104 72-73 -0.943785555477298 1104 74-75 -0.9092759789003608 1104 76-77 -0.8434110334226155 1104 78-79 -0.7130574514961978 1104 80-81 -0.6703094893279555 1104 82-83 -0.6896063338821037 1104 84-85 -0.7213567222384007 1104 86-87 -0.856516395244558 1104 88-89 -0.8139888774747845 1104 90-91 -0.807333952765223 1104 92-93 -0.914901869039479 1104 94-95 -1.0124152264161133 1104 96-97 -0.8885142103387089 1104 98-99 -0.9305242472800508 1104 100 -0.88929872308276 1105 1 -0.3656006511977594 1105 2 -0.36117366374776694 1105 3 -0.39801944045808924 1105 4 -0.23152345566096244 1105 5 -0.27018573244413346 1105 6 -0.3758099090951319 1105 7 -0.35599419839154933 1105 8 -0.3977440567021091 1105 9 -0.5374715567827977 1105 10-11 -0.5121570269048092 1105 12-13 -0.5368929986941993 1105 14-15 -0.7728375762935542 1105 16-17 -0.6914250212640312 1105 18-19 -0.6808216935776628 1105 20-21 -0.7929249935616696 1105 22-23 -0.7251582895002073 1105 24-25 -0.7593526692085675 1105 26-27 -0.7993930972880179 1105 28-29 -0.7410073916269297 1105 30-31 -0.7189246506161098 1105 32-33 -0.7808671549535902 1105 34-35 -0.7615621764458851 1105 36-37 -0.7054089015787994 1105 38-39 -0.7926136695936279 1105 40-41 -0.8231165703729246 1105 42-43 -0.8708578254783887 1105 44-45 -0.823233166712356 1105 46-47 -0.7754605423122669 1105 48-49 -0.834341403460769 1105 50-51 -0.8654130426960265 1105 52-53 -0.8364396148966904 1105 54-55 -0.9355938696421546 1105 56-57 -0.841993857376643 1105 58-59 -0.9852135259251966 1105 60-61 -0.9014353893437814 1105 62-63 -1.00739314370891 1105 64-65 -0.8139602982957541 1105 66-67 -0.8727876087339226 1105 68-69 -0.9476680505945723 1105 70-71 -0.8904916979015383 1105 72-73 -0.8679002682577774 1105 74-75 -0.8852535054169692 1105 76-77 -0.8828304684350421 1105 78-79 -0.8499638469874959 1105 80-81 -0.8066895782417376 1105 82-83 -0.7565680379462307 1105 84-85 -0.6938673113797158 1105 86-87 -0.7529199377936528 1105 88-89 -0.8489750503315747 1105 90-91 -0.9204209062430095 1105 92-93 -0.8967179505321106 1105 94-95 -0.9201505503913303 1105 96-97 -0.9210191986118872 1105 98-99 -0.9926924833566417 1105 100 -0.9407777023245423 1106 1 -0.4035627678626099 1106 2 -0.38179395731974 1106 3 -0.41017430240286856 1106 4 -0.2789792561629838 1106 5 -0.2918083587673834 1106 6 -0.3198064849982245 1106 7 -0.36135645693133256 1106 8 -0.35606352085140003 1106 9 -0.5331013996290253 1106 10-11 -0.522571022048318 1106 12-13 -0.6289786966097282 1106 14-15 -0.6651398381073719 1106 16-17 -0.7131736639029072 1106 18-19 -0.6932164810380996 1106 20-21 -0.7069868114706566 1106 22-23 -0.6485328666037731 1106 24-25 -0.6687667189193576 1106 26-27 -0.7161357034213793 1106 28-29 -0.7097920077460955 1106 30-31 -0.7001398639741794 1106 32-33 -0.6790238039768397 1106 34-35 -0.6932447926741858 1106 36-37 -0.7597886080068221 1106 38-39 -0.7164218860620295 1106 40-41 -0.7285427749456588 1106 42-43 -0.6715608907954262 1106 44-45 -0.7174352225346823 1106 46-47 -0.8155495892946973 1106 48-49 -0.8025982516545866 1106 50-51 -0.8738450518633272 1106 52-53 -0.7598068595218166 1106 54-55 -0.8716190183379879 1106 56-57 -0.8078494041408035 1106 58-59 -0.8412191668179219 1106 60-61 -0.8696920670148458 1106 62-63 -0.8188931982761858 1106 64-65 -0.7071081824494172 1106 66-67 -0.7293269858484095 1106 68-69 -0.7796252084577162 1106 70-71 -0.8088658657504979 1106 72-73 -0.8857115404939364 1106 74-75 -0.8769386453820474 1106 76-77 -0.8127756283171763 1106 78-79 -0.7485242938935315 1106 80-81 -0.5989398306154143 1106 82-83 -0.7250624243371533 1106 84-85 -0.795951269963151 1106 86-87 -0.7783119696063778 1106 88-89 -0.8568821904603539 1106 90-91 -0.8814054705232692 1106 92-93 -0.8503152985622329 1106 94-95 -0.8369088446181117 1106 96-97 -0.9369073152221681 1106 98-99 -0.9238497189781611 1106 100 -0.8759940615955237 1107 1 -0.355478733696021 1107 2 -0.4103628506485322 1107 3 -0.4256114777996203 1107 4 -0.2280162880373382 1107 5 -0.2522412555964735 1107 6 -0.36342725676664145 1107 7 -0.3821707851639431 1107 8 -0.4248185544604226 1107 9 -0.51294457044132 1107 10-11 -0.5554805480888589 1107 12-13 -0.5880970793498719 1107 14-15 -0.7511758645505964 1107 16-17 -0.7333698100944019 1107 18-19 -0.6406155733106047 1107 20-21 -0.6735195550973359 1107 22-23 -0.7468922327304242 1107 24-25 -0.7031624502898453 1107 26-27 -0.7606551357335363 1107 28-29 -0.7255810679067665 1107 30-31 -0.7575428831070568 1107 32-33 -0.702760981180532 1107 34-35 -0.7756731250524282 1107 36-37 -0.7411328268423247 1107 38-39 -0.770209338049149 1107 40-41 -0.8082778683642786 1107 42-43 -0.694175976521656 1107 44-45 -0.7477670244892707 1107 46-47 -0.8296010218081946 1107 48-49 -0.8487942629711753 1107 50-51 -0.8098667196141562 1107 52-53 -0.9003001306190868 1107 54-55 -0.8884124770985338 1107 56-57 -0.895318524885468 1107 58-59 -0.8655853031487055 1107 60-61 -0.9525346073353447 1107 62-63 -1.0725450170869095 1107 64-65 -0.9584079667786369 1107 66-67 -0.9934485984876673 1107 68-69 -0.9834255026011789 1107 70-71 -1.0957605419990912 1107 72-73 -1.0131161697363709 1107 74-75 -0.9303650327221327 1107 76-77 -0.896052559416102 1107 78-79 -0.8221731893390185 1107 80-81 -0.7717511948267131 1107 82-83 -0.7764262258477501 1107 84-85 -0.7806020052572684 1107 86-87 -0.8653957127271141 1107 88-89 -0.9042341571817474 1107 90-91 -0.9127312489257307 1107 92-93 -0.8694978662707271 1107 94-95 -0.8314150475732269 1107 96-97 -1.074318920530434 1107 98-99 -0.9710006317392654 1107 100 -0.9951717247618959 1108 1 -0.4086143703421641 1108 2 -0.4034059989973997 1108 3 -0.3988540702741439 1108 4 -0.3315755368277138 1108 5 -0.3428171625131 1108 6 -0.41600059605071493 1108 7 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2311 40-41 0.9665838006845888 2311 42-43 0.9554343823138183 2311 44-45 0.9817974455325427 2311 46-47 0.6162082888565621 2311 48-49 0.835884941622723 2311 50-51 0.9905415027585249 2311 52-53 0.9087755984613821 2311 54-55 0.9627985236788206 2311 56-57 1.0352797975398715 2311 58-59 1.0035156020896494 2311 60-61 1.1365237943296833 2311 62-63 1.054543776513725 2311 64-65 1.0483356200715832 2311 66-67 1.06701387549613 2311 68-69 1.1650360310380847 2311 70-71 1.08911312584614 2311 72-73 1.1445190267329437 2311 74-75 1.1219746949540905 2311 76-77 1.1082789935315667 2311 78-79 0.8441595296358813 2311 80-81 0.7939829635022377 2311 82-83 0.9475284370073851 2311 84-85 0.9658265661713052 2311 86-87 1.018427000981859 2311 88-89 1.065370960800152 2311 90-91 1.0869585000649664 2311 92-93 1.1100334409335666 2311 94-95 1.1339756091634001 2311 96-97 1.139139428475307 2311 98-99 1.208685995307551 2311 100 1.3695395518498543 2312 1 0.24318288935550214 2312 2 0.36472005873444147 2312 3 0.42551594384488567 2312 4 0.19397059541944373 2312 5 0.37034999337944186 2312 6 0.4527855182209777 2312 7 0.3248280355223656 2312 8 0.4404102552261904 2312 9 0.6013704114834795 2312 10-11 0.6391855182543722 2312 12-13 0.6853344540236606 2312 14-15 0.8955860753923659 2312 16-17 0.8540355238766821 2312 18-19 0.8449395742944787 2312 20-21 0.8958699002438806 2312 22-23 0.9260942603664191 2312 24-25 0.9531151401675544 2312 26-27 0.9719182495030765 2312 28-29 0.9762924726878666 2312 30-31 1.0302972362390364 2312 32-33 0.8656027220066917 2312 34-35 0.936045794829468 2312 36-37 1.0035587831884598 2312 38-39 0.8468818029508896 2312 40-41 0.9889146464557328 2312 42-43 1.107403465469467 2312 44-45 0.8188624775154878 2312 46-47 0.8642015369020513 2312 48-49 0.9812822408409119 2312 50-51 0.8871132866959712 2312 52-53 1.0168615970399202 2312 54-55 0.8461421626269328 2312 56-57 0.9083384329343289 2312 58-59 1.1365113376973213 2312 60-61 1.17726295566586 2312 62-63 1.197371068624598 2312 64-65 1.0912585056437223 2312 66-67 1.1743066971023879 2312 68-69 1.2261465498724817 2312 70-71 1.118934732100584 2312 72-73 1.0806085789717557 2312 74-75 1.1765248015638932 2312 76-77 1.1342534071776207 2312 78-79 0.9941080015619619 2312 80-81 0.9395320040139623 2312 82-83 0.9844836608879817 2312 84-85 0.9886232968038549 2312 86-87 1.0704682234409901 2312 88-89 1.0271886580282938 2312 90-91 1.111046986205693 2312 92-93 1.0870362838617815 2312 94-95 1.1529080896182684 2312 96-97 0.7918178222208674 2312 98-99 0.6056589874894982 2312 100 0.8285352874575302 2313 1 0.0444382911858483 2313 2 0.22396890121554236 2313 3 0.2902099541180405 2313 4 0.18634195729620018 2313 5 0.3144740531139476 2313 6 0.2415971603361342 2313 7 0.24860226933640206 2313 8 0.33535344849971693 2313 9 0.5116426688665214 2313 10-11 0.36516568881999234 2313 12-13 0.41200632259268133 2313 14-15 0.4899234704974802 2313 16-17 0.7361252371651048 2313 18-19 0.7130670112392821 2313 20-21 0.6836808706815134 2313 22-23 0.6276500499136333 2313 24-25 0.537865936532711 2313 26-27 0.7060415211010636 2313 28-29 0.5736543593115542 2313 30-31 0.8088071690239715 2313 32-33 0.8877817695129977 2313 34-35 0.9842285462754674 2313 36-37 1.0108576823036657 2313 38-39 1.0218123276595676 2313 40-41 1.017037793441581 2313 42-43 1.0874765960836257 2313 44-45 0.9527056637128837 2313 46-47 0.993894907506288 2313 48-49 1.0298743401296093 2313 50-51 1.0057511351456796 2313 52-53 1.0916573634885935 2313 54-55 0.978118783860495 2313 56-57 0.6979885093775238 2313 58-59 0.7968294791204187 2313 60-61 0.7233937237205126 2313 62-63 1.0395669015263707 2313 64-65 1.107704841347406 2313 66-67 1.0709413038247746 2313 68-69 1.1461097862730725 2313 70-71 1.0893154241851093 2313 72-73 1.0896433110310042 2313 74-75 1.1917173861957124 2313 76-77 1.1103002774452158 2313 78-79 1.0290812829286153 2313 80-81 0.9156601338747024 2313 82-83 1.023061162036221 2313 84-85 0.9923763264237913 2313 86-87 1.0088258113486575 2313 88-89 1.0497424075802542 2313 90-91 0.8905242131367714 2313 92-93 0.9845559286427132 2313 94-95 1.1195182882659154 2313 96-97 1.2158571839530872 2313 98-99 1.2235382218234214 2313 100 1.3177829613224112 2314 1 -0.41505589906570606 2314 2 0.0022519758683472446 2314 3 0.2665000298814846 2314 4 0.21126624532979577 2314 5 0.2792203973852807 2314 6 0.35108005377366425 2314 7 0.4553245827684975 2314 8 0.45646724248273784 2314 9 0.39317300931150356 2314 10-11 0.5736754729488567 2314 12-13 0.44624068126487515 2314 14-15 0.7483251723824225 2314 16-17 0.7943939902163564 2314 18-19 0.833753002743066 2314 20-21 0.8579173935240973 2314 22-23 0.8823187230032588 2314 24-25 0.7963987222571092 2314 26-27 0.706363893125058 2314 28-29 0.8124005858551229 2314 30-31 0.8522949782754736 2314 32-33 0.891911653539502 2314 34-35 0.9226723329209037 2314 36-37 0.9185454313702408 2314 38-39 0.698451555598588 2314 40-41 0.6229458426207088 2314 42-43 0.8265581330486853 2314 44-45 0.8764308964437717 2314 46-47 0.8699156178787035 2314 48-49 0.8065974636685809 2314 50-51 0.8127673502991897 2314 52-53 0.805058765226029 2314 54-55 0.709657332222811 2314 56-57 0.6346647671655745 2314 58-59 0.8323466818466514 2314 60-61 0.9279170923896558 2314 62-63 0.8108110702625027 2314 64-65 0.8121671897364848 2314 66-67 0.5508996289744772 2314 68-69 0.4241174537306165 2314 70-71 0.6339516522031872 2314 72-73 0.8017230243813884 2314 74-75 0.8798833761220308 2314 76-77 0.7827712529867874 2314 78-79 0.6716526709253188 2314 80-81 0.680762798893177 2314 82-83 0.7126830519397842 2314 84-85 0.7321551963888204 2314 86-87 0.8262925702548358 2314 88-89 0.825158830680607 2314 90-91 0.9158988292830728 2314 92-93 0.7902993629402282 2314 94-95 0.8235703573507429 2314 96-97 0.8341397224200477 2314 98-99 0.9383330656583269 2314 100 0.9916055914344142 2315 1 0.4145403486237811 2315 2 0.4282624844128833 2315 3 0.4279558057653645 2315 4 0.2950799139561511 2315 5 0.359656738438936 2315 6 0.4048331725227783 2315 7 0.35090722731737145 2315 8 0.2813624258221452 2315 9 0.49577627435095195 2315 10-11 0.5478559331610029 2315 12-13 0.3516782722616796 2315 14-15 0.5921578401445302 2315 16-17 0.7620294286043077 2315 18-19 0.7319764858101863 2315 20-21 0.44573787236697626 2315 22-23 0.7784841042020858 2315 24-25 0.8159246197632726 2315 26-27 0.7516997631851083 2315 28-29 0.7637300742505815 2315 30-31 0.8294093275851466 2315 32-33 0.711974989004311 2315 34-35 0.7436903618000414 2315 36-37 0.8138696666211942 2315 38-39 0.8355615592412775 2315 40-41 0.8395159347330576 2315 42-43 0.8461619593407903 2315 44-45 0.9127873253430536 2315 46-47 0.9031224532068691 2315 48-49 0.8292970701902362 2315 50-51 0.8750714522554475 2315 52-53 0.8911630627373057 2315 54-55 0.6496329010033222 2315 56-57 0.7281871106980731 2315 58-59 0.7162206484820004 2315 60-61 0.755860990430385 2315 62-63 0.8667503301683439 2315 64-65 1.0174556333824682 2315 66-67 0.9444045108952608 2315 68-69 0.9334967639198908 2315 70-71 0.8949754364596103 2315 72-73 0.9620902595560281 2315 74-75 0.36172719074549775 2315 76-77 0.36858530054326977 2315 78-79 0.6934586526832902 2315 80-81 0.6986726044997695 2315 82-83 0.7169292121708324 2315 84-85 0.7683840183108366 2315 86-87 0.6907218762901621 2315 88-89 0.7127889422432077 2315 90-91 0.8704053348142118 2315 92-93 0.8307204001787589 2315 94-95 0.9349411183048488 2315 96-97 1.060720938086554 2315 98-99 1.0834759181827032 2315 100 1.0393568403437676 2316 1 0.25817879994215787 2316 2 0.03111708997501239 2316 3 0.17832264807694287 2316 4 -0.030237235531657802 2316 5 0.25484043711136906 2316 6 0.3595216427469765 2316 7 0.42829794646706176 2316 8 0.4378349811606981 2316 9 0.6286685874868567 2316 10-11 0.6347732782980486 2316 12-13 0.4113001211469296 2316 14-15 0.5410054522872727 2316 16-17 0.8012104423032227 2316 18-19 0.7446234395446965 2316 20-21 0.7375275425037557 2316 22-23 0.7706139056736205 2316 24-25 0.4520664793329132 2316 26-27 0.7620095064475194 2316 28-29 0.9070995078738022 2316 30-31 0.5612632081350526 2316 32-33 0.6961365938830539 2316 34-35 0.7404347446897575 2316 36-37 0.850636285531877 2316 38-39 0.7338508425892414 2316 40-41 0.7637616965335852 2316 42-43 0.7373051891488487 2316 44-45 0.6107278049611935 2316 46-47 0.7905712427139662 2316 48-49 0.8725306941066151 2316 50-51 0.8476025081022058 2316 52-53 0.923902221376224 2316 54-55 0.8097782156551645 2316 56-57 0.959338527247084 2316 58-59 0.7637280286902097 2316 60-61 0.8719123795746242 2316 62-63 0.8984696151467233 2316 64-65 0.9859418841673318 2316 66-67 0.9628992606272817 2316 68-69 0.9126752105898603 2316 70-71 0.9056953840089719 2316 72-73 0.8887703626534673 2316 74-75 0.8356328212112771 2316 76-77 0.7935508711348298 2316 78-79 0.5764533000810488 2316 80-81 0.5646112525080653 2316 82-83 0.4385574716905225 2316 84-85 0.5382004410519379 2316 86-87 0.6048764391841033 2316 88-89 0.5498519836174687 2316 90-91 0.5523478472417551 2316 92-93 0.4103104387188843 2316 94-95 0.46537081813591286 2316 96-97 0.6383241309155245 2316 98-99 0.7827025139901203 2316 100 0.3835919524431475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 5.0 12 27.0 13 147.0 14 621.0 15 1820.0 16 4390.0 17 9159.0 18 15319.0 19 21750.0 20 29246.0 21 39524.0 22 47112.0 23 49824.0 24 49504.0 25 52244.0 26 60348.0 27 73058.0 28 89749.0 29 110373.0 30 136710.0 31 169776.0 32 216697.0 33 283621.0 34 403472.0 35 561002.0 36 868992.0 37 1328411.0 38 1151223.0 39 176502.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36620010511001 19.526352652480963 13.231335828491133 23.876111413917897 2 15.775998811557802 20.190191083423365 40.54370894459039 23.490101160428438 3 19.01946124513855 25.848080049110628 29.61046084568619 25.521997860064626 4 12.56843589857812 16.43530686363587 38.10212622177694 32.89413101600907 5 13.074578276950389 37.40589888796966 34.37305220760029 15.146470627479665 6 30.584237310665547 36.636806048149055 18.43602888895947 14.342927752225934 7 27.461800865896898 32.13153714260075 22.360410130553376 18.046251860948985 8 26.215787963897036 35.44216284380505 20.078018407074055 18.26403078522386 9 26.61493062289977 16.04243130946062 20.865197768181424 36.47744029945818 10-11 25.5426128517349 26.052332119203882 27.73081028331713 20.674244745744083 12-13 25.595208586632207 23.724856717213008 28.205253380095773 22.474681316059016 14-15 22.845253612785793 25.178390580570664 26.263282927801086 25.713072878842457 16-17 22.08086087041058 27.695940017092312 27.19198484664267 23.031214265854437 18-19 21.676600880542907 27.09741478531662 29.349984968021126 21.87599936611935 20-21 22.7309037313683 26.447021587826057 29.109547863336843 21.712526817468795 22-23 22.63462275614736 26.23409689736259 28.8928582051666 22.238422141323454 24-25 22.110767850692444 26.18372398893459 29.452805436209843 22.252702724163118 26-27 22.10798857026759 26.418253356939836 28.973205399336088 22.500552673456493 28-29 21.811926106367313 26.973459720975974 29.045516070597177 22.169098102059536 30-31 22.635251807628805 26.503119498863537 29.06171356068213 21.799915132825532 32-33 22.568605232001925 26.572963776948455 28.671248864647108 22.18718212640251 34-35 22.581935147791256 26.678707245586036 28.943506017221743 21.795851589400964 36-37 22.421407443885755 26.593731826491833 28.71671277311166 22.26814795651075 38-39 22.394936513112555 26.78176781650155 28.86827101185694 21.95502465852896 40-41 22.477190798377094 26.167339908737986 29.102828826669896 22.252640466215023 42-43 22.350573463382055 26.439527570262804 28.61865397201293 22.59124499434221 44-45 22.14972958609925 26.95520452010293 28.7229999968067 22.172065896991118 46-47 22.179818458554138 26.582663681903362 28.269110414307768 22.968407445234728 48-49 22.501540859562258 26.651219558307933 28.323809483796293 22.52343009833352 50-51 22.42290851813059 26.81263050542997 28.14845820397637 22.616002772463066 52-53 22.198360258435265 27.149450702374235 27.839581160350267 22.812607878840236 54-55 22.05734812023776 26.968352659821786 28.07426124511544 22.90003797482502 56-57 22.55905456566329 26.894292266544685 27.687806286387495 22.85884688140453 58-59 22.05095613551239 27.047470478637493 28.162830259266318 22.738743126583795 60-61 22.436838289029293 27.30794526667083 27.780598811009416 22.474617633290457 62-63 22.51537358537177 27.04617516287367 27.624853007223283 22.81359824453128 64-65 22.088930688187496 26.903135673064853 27.762103974737297 23.245829664010355 66-67 22.103681042046617 26.774118531726398 27.76516184619441 23.357038580032572 68-69 21.867054751878946 26.88018079255643 27.91074208265391 23.342022372910712 70-71 21.981779810201733 26.648872868922442 27.9163467885583 23.453000532317525 72-73 22.137185439888608 26.909796973713124 27.75144844046242 23.201569145935853 74-75 22.279507876833545 27.058612504237377 27.480051236002577 23.181828382926497 76-77 22.127631247498957 26.871527486225936 27.444183634248454 23.55665763202665 78-79 21.9548220728869 26.930394360385552 27.602319277576715 23.512464289150834 80-81 21.805215765739575 27.195897404183196 27.613865485917827 23.385021344159405 82-83 22.310804023796464 26.801703830255807 28.019694495754084 22.867797650193648 84-85 22.446952866169 27.06603686265193 27.32604244981286 23.160967821366206 86-87 21.759435772599005 27.337301835849274 27.60323998622892 23.3000224053228 88-89 21.99344761497322 26.749114652780264 27.609186657713558 23.648251074532958 90-91 22.1279203920275 26.7192227105865 27.66363930134205 23.489217596043947 92-93 22.172636684690282 26.85636434030725 27.574039134160362 23.396959840842108 94-95 21.586266214162453 26.848102536995665 27.79341168541573 23.77221956342615 96-97 21.63531883467219 26.82637158791455 27.6387943926231 23.89951518479016 98-99 21.386472092965207 27.01693541209486 27.47852071834917 24.118071776590764 100 21.5373008603281 26.853771792084324 27.3915798154814 24.217347532106178 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1330.0 1 995.0 2 1672.5 3 2492.5 4 1691.5 5 1198.5 6 1660.5 7 2852.0 8 4290.0 9 4976.0 10 4549.0 11 3995.0 12 4249.0 13 4888.0 14 5492.0 15 6141.0 16 7014.5 17 7911.0 18 8818.0 19 9636.5 20 10486.5 21 11709.5 22 13279.0 23 15582.5 24 18443.5 25 21823.0 26 26600.5 27 32450.5 28 39526.5 29 49256.0 30 61805.5 31 74031.5 32 89145.0 33 104025.0 34 116282.0 35 129521.5 36 142055.5 37 158646.0 38 168902.0 39 170074.5 40 169335.5 41 167598.0 42 170177.5 43 177478.5 44 189077.5 45 208521.5 46 226147.5 47 239351.5 48 250795.0 49 254733.0 50 252502.0 51 251634.5 52 246787.5 53 234888.0 54 222501.0 55 201136.0 56 178303.0 57 154303.0 58 130227.0 59 114069.0 60 92753.0 61 70072.5 62 54646.5 63 42829.5 64 31357.5 65 20798.5 66 14518.5 67 10994.5 68 8424.0 69 6302.0 70 4860.5 71 3761.5 72 2971.0 73 2352.5 74 1790.0 75 1331.5 76 918.5 77 570.5 78 346.5 79 238.5 80 164.0 81 90.5 82 51.0 83 35.0 84 20.0 85 14.0 86 12.5 87 7.5 88 5.5 89 4.5 90 1.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04357509359906578 2 0.0 3 0.0 4 0.03078656825047764 5 0.0 6 0.0 7 9.074643479944281E-4 8 0.0 9 0.011780231628594337 10-11 3.108905636647578E-4 12-13 3.3609790666460306E-5 14-15 1.7645140099891658E-4 16-17 3.108905636647578E-4 18-19 0.0 20-21 2.5207342999845226E-5 22-23 0.0 24-25 5.125493076635196E-4 26-27 4.4532972633059897E-4 28-29 2.1006119166537687E-4 30-31 0.002890441997315586 32-33 0.016048675043234796 34-35 0.026845820294835166 36-37 0.03213936232480266 38-39 0.02330838982719022 40-41 0.0014788307893242533 42-43 9.242692433276583E-5 44-45 0.011662597361261724 46-47 0.01842656773288686 48-49 0.00394074795564247 50-51 0.011813841419260796 52-53 0.011511353303262653 54-55 0.010965194204932674 56-57 4.621346216638292E-4 58-59 0.009225887537943353 60-61 0.0051338955243018115 62-63 0.0016720870856564002 64-65 0.035550756077448384 66-67 0.06017833018829717 68-69 0.05880873121863891 70-71 0.05157422377768333 72-73 0.04533960760905495 74-75 0.035214658170783786 76-77 0.03153438609280638 78-79 0.01595624811890203 80-81 0.001226757359325801 82-83 0.03839078338876428 84-85 0.051801089864681946 86-87 0.0571282416853159 88-89 0.06906811981957592 90-91 0.04875100136170067 92-93 0.039323455079758554 94-95 0.013351489342251357 96-97 0.003957552850975701 98-99 0.02298069436819223 100 0.03142515427314038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5950647.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.26759092864071 #Duplication Level Percentage of deduplicated Percentage of total 1 79.50823651820087 53.4832752956395 2 13.10591837911819 17.632071125413525 3 3.695377227860374 7.457373710721781 4 1.4147240066186693 3.8066030301660905 5 0.7185716731737876 2.4168292681981627 6 0.41657625864535924 1.6813248814283792 7 0.23866820524933371 1.1238244638869528 8 0.17331093712314138 0.9326567377487079 9 0.12707031817317196 0.7692942763840637 >10 0.5634832705893033 6.422848639261504 >50 0.025172953959671996 1.1454160508591498 >100 0.011766042751965149 1.4251027282784843 >500 6.508589871169079E-4 0.2884814305651278 >1k 3.8459650864674957E-4 0.5094971426925269 >5k 0.0 0.0 >10k+ 8.875304044689064E-5 0.9054012187560264 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19452 0.32688882402199293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15605 0.2622403916750565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10439 0.17542630238358956 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.680489533323015E-5 0.0 6 0.0 0.0 0.0 1.680489533323015E-5 0.0 7 0.0 0.0 0.0 1.680489533323015E-5 0.0 8 0.0 0.0 0.0 1.680489533323015E-5 1.680489533323015E-5 9 0.0 0.0 0.0 1.680489533323015E-5 1.680489533323015E-5 10-11 0.0 0.0 0.0 2.5207342999845226E-5 1.680489533323015E-5 12-13 0.0 0.0 0.0 3.36097906664603E-5 3.36097906664603E-5 14-15 0.0 0.0 0.0 5.041468599969045E-5 6.72195813329206E-5 16-17 0.0 0.0 0.0 5.041468599969045E-5 8.402447666615076E-5 18-19 0.0 0.0 0.0 6.721958133292061E-5 8.402447666615076E-5 20-21 0.0 0.0 0.0 1.1763426733261106E-4 8.402447666615076E-5 22-23 6.72195813329206E-5 0.0 0.0 3.0248811599814274E-4 1.008293719993809E-4 24-25 6.72195813329206E-5 0.0 0.0 8.402447666615076E-4 1.1763426733261106E-4 26-27 6.72195813329206E-5 0.0 0.0 0.0017309042193227055 1.1763426733261106E-4 28-29 6.72195813329206E-5 0.0 0.0 0.004780992722303978 1.1763426733261106E-4 30-31 6.72195813329206E-5 0.0 0.0 0.017485493594225972 1.1763426733261106E-4 32-33 6.72195813329206E-5 0.0 0.0 0.04717974364804365 1.8485384866553166E-4 34-35 6.72195813329206E-5 0.0 0.0 0.09049436136944436 2.016587439987618E-4 36-37 6.72195813329206E-5 0.0 0.0 0.15813406508569572 2.016587439987618E-4 38-39 6.72195813329206E-5 0.0 0.0 0.2611984881643963 2.1846363933199198E-4 40-41 6.72195813329206E-5 0.0 0.0 0.37886636528767376 2.520734299984523E-4 42-43 6.72195813329206E-5 0.0 0.0 0.4923162136823105 2.8568322066491257E-4 44-45 6.72195813329206E-5 0.0 0.0 0.6215374563471838 2.940856683315276E-4 46-47 6.72195813329206E-5 0.0 0.0 0.7665132883869603 3.1929301133137286E-4 48-49 6.72195813329206E-5 0.0 0.0 0.9257984888029822 3.697076973310633E-4 50-51 6.72195813329206E-5 0.0 0.0 1.0930660145022886 3.697076973310633E-4 52-53 6.72195813329206E-5 0.0 0.0 1.2570061709256155 4.117199356641387E-4 54-55 7.562202899953567E-5 0.0 0.0 1.4423977762418103 4.537321739972141E-4 56-57 8.402447666615076E-5 0.0 0.0 1.6498037944445367 4.537321739972141E-4 58-59 8.402447666615076E-5 0.0 0.0 1.887971173554741 4.705370693304442E-4 60-61 8.402447666615076E-5 0.0 0.0 2.124651319427955 4.873419646636744E-4 62-63 8.402447666615076E-5 0.0 0.0 2.3685659727421235 4.873419646636744E-4 64-65 8.402447666615076E-5 0.0 0.0 2.6143711767812814 4.873419646636744E-4 66-67 8.402447666615076E-5 0.0 0.0 2.8897950088452564 5.041468599969045E-4 68-69 8.402447666615076E-5 0.0 0.0 3.1988874487093586 5.125493076635196E-4 70-71 8.402447666615076E-5 0.0 0.0 3.521843927223376 5.209517553301347E-4 72-73 8.402447666615076E-5 0.0 0.0 3.8378851913077687 5.54561545996595E-4 74-75 8.402447666615076E-5 0.0 0.0 4.158984728887464 5.629639936632101E-4 76-77 8.402447666615076E-5 8.402447666615075E-6 0.0 4.50267004579502 5.713664413298251E-4 78-79 8.402447666615076E-5 1.680489533323015E-5 0.0 4.875889966250728 5.713664413298251E-4 80-81 8.402447666615076E-5 1.680489533323015E-5 0.0 5.260175910283369 6.049762319962854E-4 82-83 1.0923181966599599E-4 1.680489533323015E-5 0.0 5.65404064465595 6.890007086624362E-4 84-85 1.1763426733261106E-4 1.680489533323015E-5 0.0 6.043804984567224 6.890007086624362E-4 86-87 1.1763426733261106E-4 1.680489533323015E-5 0.0 6.457297836689019 6.890007086624362E-4 88 1.1763426733261106E-4 1.680489533323015E-5 0.0 6.794286402806283 6.890007086624362E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9890 0.0 48.531998 1 TACCTGG 5435 0.0 44.35636 2 GTATCAA 17375 0.0 40.23323 1 GTACCTG 6515 0.0 37.666462 1 ACCTGGG 6830 0.0 34.612198 3 ATCAACG 21025 0.0 32.211422 3 CAACGCA 21435 0.0 31.717785 5 TATCAAC 22400 0.0 30.965382 2 GTGGTAT 3945 0.0 30.506449 1 TCAACGC 22815 0.0 29.849003 4 AACGCAG 23415 0.0 29.597544 6 GTACATG 20845 0.0 29.386053 1 GTACTAG 3020 0.0 29.109411 1 CCTGGGG 6515 0.0 28.350456 4 TACATGG 21035 0.0 28.260908 2 ACATGGG 21035 0.0 26.990211 3 ACGCAGA 25760 0.0 26.483692 7 CTGTGCG 4150 0.0 26.266224 9 CGCAGAG 26155 0.0 26.011654 8 TGGGGGG 12935 0.0 25.46299 6 >>END_MODULE