FastQCFastQC Report
Mon 6 Jun 2016
ERR1211187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5561190
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165730.29801175647658146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127510.22928545868779884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86640.15579399373155745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76200.046.931821
GTATCAA138050.042.0276831
CAACGCA163200.034.506725
TACCTGG43700.034.4566762
ATCAACG163250.034.410083
TATCAAC176250.032.7849162
AACGCAG179350.031.9763876
TCAACGC182000.031.2780594
GTACTAG30850.029.8957631
GTACATG177500.029.5586951
CTAACGC13700.029.1654933
GTACCTG53600.028.9706291
TACATGG178600.028.8494472
ACGCAGA195600.028.839217
CGCAGAG200850.028.1060078
TAACGCC14400.028.0739174
ACATGGG178450.028.0282523
TCTAACG14900.026.8433972
ACCTGGG55350.026.8373993
CATGGGG140800.026.1746234
Produced by FastQC (version 0.11.5)