##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211187_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5561190 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.761855106550936 31.0 31.0 34.0 30.0 34.0 2 31.789268304085997 33.0 31.0 34.0 30.0 34.0 3 31.86266086934631 33.0 31.0 34.0 30.0 34.0 4 35.46037179092964 37.0 35.0 37.0 33.0 37.0 5 35.51259910199076 37.0 35.0 37.0 33.0 37.0 6 35.57526662458934 37.0 35.0 37.0 33.0 37.0 7 35.50707924023455 37.0 35.0 37.0 33.0 37.0 8 35.50874632947265 37.0 35.0 37.0 33.0 37.0 9 37.1357980935735 39.0 37.0 39.0 33.0 39.0 10-11 37.08766181338886 39.0 37.0 39.0 33.0 39.0 12-13 36.99406269161816 39.0 37.0 39.0 33.0 39.0 14-15 38.25902873305893 40.0 38.0 41.0 33.0 41.0 16-17 38.230056786407225 40.0 38.0 41.0 33.0 41.0 18-19 38.23125149473404 40.0 38.0 41.0 33.5 41.0 20-21 38.19254592991788 40.0 38.0 41.0 33.5 41.0 22-23 38.08875798165501 40.0 38.0 41.0 33.0 41.0 24-25 38.00267712485997 40.0 38.0 41.0 33.0 41.0 26-27 37.86586018460078 40.0 37.5 41.0 33.0 41.0 28-29 37.74668272438093 40.0 37.0 41.0 32.5 41.0 30-31 37.595598424078304 40.0 37.0 41.0 32.0 41.0 32-33 37.41289517171684 40.0 36.5 41.0 32.0 41.0 34-35 37.260780876035525 40.0 36.0 41.0 31.0 41.0 36-37 37.14813601045819 40.0 36.0 41.0 31.0 41.0 38-39 37.01193548503108 39.0 36.0 41.0 31.0 41.0 40-41 36.85296833951007 39.0 36.0 41.0 30.0 41.0 42-43 36.65342130011742 39.0 35.0 41.0 30.0 41.0 44-45 36.452429336167256 39.0 35.0 40.0 30.0 41.0 46-47 36.23254609175375 39.0 35.0 40.0 29.0 41.0 48-49 36.044877535203796 39.0 35.0 40.0 28.0 41.0 50-51 35.38102977959753 38.0 34.0 39.5 27.5 40.5 52-53 35.49367284699857 38.0 34.5 40.0 27.5 40.5 54-55 35.97903865899205 39.0 35.0 40.0 28.0 41.0 56-57 35.94904795556347 38.5 35.0 40.0 28.0 41.0 58-59 35.745635286692234 38.0 35.0 40.0 28.0 41.0 60-61 35.51710191523757 38.0 34.0 40.0 28.0 41.0 62-63 35.21582574952483 37.0 34.0 40.0 27.0 41.0 64-65 34.96305808648869 37.0 34.0 40.0 27.0 41.0 66-67 34.785652980746924 36.5 34.0 40.0 27.0 41.0 68-69 34.48372794312009 36.0 34.0 39.0 26.0 41.0 70-71 34.15645518315324 35.5 33.5 39.0 26.0 41.0 72-73 33.80430240649933 35.0 33.0 38.5 26.0 40.0 74-75 33.46344118794718 35.0 33.0 37.5 25.5 40.0 76-77 33.11542035068034 35.0 33.0 37.0 25.0 39.0 78-79 32.771253364837385 35.0 32.5 36.5 25.0 39.0 80-81 32.46883580672482 35.0 32.0 36.0 24.0 38.5 82-83 32.1695143485477 35.0 32.0 36.0 24.0 37.0 84-85 31.914370934997727 35.0 32.0 35.0 23.0 37.0 86-87 31.686384838496796 35.0 31.5 35.0 23.0 36.5 88-89 31.468018985145264 35.0 31.0 35.0 22.5 36.0 90-91 31.245155982802242 34.0 31.0 35.0 20.0 36.0 92-93 31.055445147531373 34.0 31.0 35.0 20.0 36.0 94-95 30.880509657105762 34.0 31.0 35.0 20.0 35.0 96-97 30.70072115860095 34.0 30.5 35.0 19.0 35.0 98-99 30.472461739303995 34.0 30.0 35.0 18.5 35.0 100 29.35538203154361 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3273659678331171 1101 2 -0.24716453943094763 1101 3 -0.2606727137489955 1101 4 -0.16273725370368908 1101 5 -0.07420798816951901 1101 6 -0.10488964300686376 1101 7 -0.14407897233513012 1101 8 -0.13379015064599287 1101 9 -0.2747842886362335 1101 10-11 -0.27417883666216625 1101 12-13 -0.23589499440563344 1101 14-15 -0.2933331469600162 1101 16-17 -0.3490854600945852 1101 18-19 -0.353801253974666 1101 20-21 -0.4678190270203757 1101 22-23 -0.4511514797399556 1101 24-25 -0.5539297708328021 1101 26-27 -0.5495912993228913 1101 28-29 -0.3612766138949155 1101 30-31 -0.5350185514334029 1101 32-33 -0.5555444872112858 1101 34-35 -0.560364102020273 1101 36-37 -0.6084899256315452 1101 38-39 -0.5429754840013459 1101 40-41 -0.6244428501142565 1101 42-43 -0.7374492002526836 1101 44-45 -0.769510180228302 1101 46-47 -0.8316418617633872 1101 48-49 -0.9513369850756206 1101 50-51 -0.9175615975249016 1101 52-53 -0.785141416596737 1101 54-55 -0.7491586466453626 1101 56-57 -0.572786529655609 1101 58-59 -0.5995962274964484 1101 60-61 -0.603811529447519 1101 62-63 -0.7206990893663701 1101 64-65 -0.6175811285431294 1101 66-67 -0.7372979713769894 1101 68-69 -0.6208007707256726 1101 70-71 -0.7265641207877849 1101 72-73 -0.7830421400823155 1101 74-75 -0.7954500538390903 1101 76-77 -0.7639327432118108 1101 78-79 -0.8467105988747967 1101 80-81 -0.8521997424808347 1101 82-83 -0.8212643858667725 1101 84-85 -0.9631647927030755 1101 86-87 -0.9432846392526422 1101 88-89 -1.0533895859126048 1101 90-91 -1.114561135277846 1101 92-93 -1.1615820627711066 1101 94-95 -1.2068440469352169 1101 96-97 -1.2263702761102024 1101 98-99 -1.1623190556749847 1101 100 -1.4225901775236274 1102 1 -0.3810830592915835 1102 2 -0.30306853928173183 1102 3 -0.3906442699511423 1102 4 -0.17035571512737135 1102 5 -0.2154796590794419 1102 6 -0.2551840596292365 1102 7 -0.2806196469660591 1102 8 -0.2910468629159979 1102 9 -0.35952423771717434 1102 10-11 -0.3815282011064909 1102 12-13 -0.39975519080773125 1102 14-15 -0.5141753631515371 1102 16-17 -0.5529146947232775 1102 18-19 -0.5638998280544385 1102 20-21 -0.6364205713787143 1102 22-23 -0.5760202186072334 1102 24-25 -0.5152877989781715 1102 26-27 -0.6032774290079601 1102 28-29 -0.6391954138464158 1102 30-31 -0.6650322604113796 1102 32-33 -0.758136649779253 1102 34-35 -0.8050023062374194 1102 36-37 -0.7590906493163629 1102 38-39 -0.8514388194796112 1102 40-41 -0.8720521722192842 1102 42-43 -0.8351995756175654 1102 44-45 -0.8652278498885622 1102 46-47 -0.8610846431005257 1102 48-49 -0.8897646026044512 1102 50-51 -0.88528143077896 1102 52-53 -0.7130949086557834 1102 54-55 -0.5903798181567979 1102 56-57 -0.5991822341758493 1102 58-59 -0.5631433089762652 1102 60-61 -0.44140322572371105 1102 62-63 -0.5651652319210925 1102 64-65 -0.5146377083862674 1102 66-67 -0.6306136788814882 1102 68-69 -0.6396991564235748 1102 70-71 -0.6378096327295211 1102 72-73 -0.6424671557030877 1102 74-75 -0.7408226675909617 1102 76-77 -0.7613260789698586 1102 78-79 -0.7498570424674824 1102 80-81 -0.7784267892685506 1102 82-83 -0.8485673542801706 1102 84-85 -0.7954870189664938 1102 86-87 -0.9980683731306037 1102 88-89 -1.0208315559020704 1102 90-91 -1.0512168440879606 1102 92-93 -1.0647859535096238 1102 94-95 -1.0747329575843878 1102 96-97 -1.139242540143254 1102 98-99 -1.183468931175085 1102 100 -1.2109984439440922 1103 1 -0.33031280758444836 1103 2 -0.3374017996180072 1103 3 -0.40867976421866103 1103 4 -0.2529993589636561 1103 5 -0.2500403605590691 1103 6 -0.27769029202975304 1103 7 -0.2794562507888969 1103 8 -0.27125744837454135 1103 9 -0.4376820573613429 1103 10-11 -0.4242749920008819 1103 12-13 -0.4245475805323977 1103 14-15 -0.5726354348656173 1103 16-17 -0.6184348430179085 1103 18-19 -0.6015498978440519 1103 20-21 -0.6625823859529092 1103 22-23 -0.754328589158014 1103 24-25 -0.6407940335907227 1103 26-27 -0.7310028848524794 1103 28-29 -0.7498623636531931 1103 30-31 -0.6948236215411114 1103 32-33 -0.7732159400708625 1103 34-35 -0.8054530741443671 1103 36-37 -0.7186039801279804 1103 38-39 -0.8201372818960877 1103 40-41 -0.7822750786967774 1103 42-43 -0.8889843625878058 1103 44-45 -0.9779966505026181 1103 46-47 -0.9366577474442934 1103 48-49 -1.069662849126594 1103 50-51 -0.9435412472245446 1103 52-53 -0.8844222164136042 1103 54-55 -0.7384473647698471 1103 56-57 -0.6682145143606775 1103 58-59 -0.6818972933991461 1103 60-61 -0.6151479749124462 1103 62-63 -0.6522034536242245 1103 64-65 -0.6693814997650875 1103 66-67 -0.7524458829724239 1103 68-69 -0.69701963342618 1103 70-71 -0.7257039772269778 1103 72-73 -0.7509459412089683 1103 74-75 -0.7490695215282699 1103 76-77 -0.721602671467096 1103 78-79 -0.7311512582246706 1103 80-81 -0.8082299405295217 1103 82-83 -0.7926653428793529 1103 84-85 -0.9204038797389806 1103 86-87 -0.8648778043507868 1103 88-89 -0.8284449944396499 1103 90-91 -1.0201242771795194 1103 92-93 -1.1189231171977845 1103 94-95 -1.1322312598497248 1103 96-97 -1.174944052952025 1103 98-99 -1.2315157952877378 1103 100 -1.2302890010001448 1104 1 -0.530585510471127 1104 2 -0.44632793095584944 1104 3 -0.4620729961503258 1104 4 -0.33249027269623355 1104 5 -0.3123803389093496 1104 6 -0.3640156189044461 1104 7 -0.4343091461670596 1104 8 -0.39197126908050706 1104 9 -0.4920729227473686 1104 10-11 -0.49231447450103616 1104 12-13 -0.48753220118123153 1104 14-15 -0.6417095577833152 1104 16-17 -0.7171005239261703 1104 18-19 -0.7543750318679869 1104 20-21 -0.7432154848425938 1104 22-23 -0.6388211990790538 1104 24-25 -0.7524518790410113 1104 26-27 -0.8160820659354471 1104 28-29 -0.8766606775174992 1104 30-31 -0.8811048207428982 1104 32-33 -0.8308833251130707 1104 34-35 -0.9869532284767857 1104 36-37 -0.9854155050895059 1104 38-39 -0.9334914453564949 1104 40-41 -0.9283961228165012 1104 42-43 -1.0233763584782665 1104 44-45 -1.01643475111009 1104 46-47 -0.9608685059428126 1104 48-49 -0.998199639575283 1104 50-51 -1.10492282727418 1104 52-53 -0.9715257474738053 1104 54-55 -0.7028139342424922 1104 56-57 -0.8124926808051285 1104 58-59 -0.7637389049785455 1104 60-61 -0.8054709649507075 1104 62-63 -0.7557684116832846 1104 64-65 -0.7723360372505965 1104 66-67 -0.8055540921964024 1104 68-69 -0.9014493798736396 1104 70-71 -0.8226318206442542 1104 72-73 -0.8400961473338668 1104 74-75 -0.8704774710903322 1104 76-77 -0.8515725511672514 1104 78-79 -0.8634032627757087 1104 80-81 -0.9617343919878714 1104 82-83 -0.9372037671617264 1104 84-85 -0.8964547609019817 1104 86-87 -1.0150683189608927 1104 88-89 -1.0757280917488785 1104 90-91 -1.080828176392071 1104 92-93 -1.152785330557915 1104 94-95 -1.1457050330969665 1104 96-97 -1.196022692360149 1104 98-99 -1.1153811220691523 1104 100 -1.1416064989805221 1105 1 -0.44317155207492576 1105 2 -0.4514940197349766 1105 3 -0.48207734826962323 1105 4 -0.3331574607960661 1105 5 -0.29367956969279163 1105 6 -0.33993680038165763 1105 7 -0.33271076876851424 1105 8 -0.37938348252322385 1105 9 -0.5374170788981303 1105 10-11 -0.5546321525222169 1105 12-13 -0.5277931538437244 1105 14-15 -0.6973471432603731 1105 16-17 -0.6685990283983827 1105 18-19 -0.6876154207667966 1105 20-21 -0.7070051337784804 1105 22-23 -0.7195442872772091 1105 24-25 -0.7512026976294877 1105 26-27 -0.7379389427971574 1105 28-29 -0.7512116086041516 1105 30-31 -0.7152177377097999 1105 32-33 -0.7564241067290638 1105 34-35 -0.7345267166798735 1105 36-37 -0.7593490818951665 1105 38-39 -0.7473192779805515 1105 40-41 -0.8871046211505842 1105 42-43 -0.7930491694341129 1105 44-45 -0.8368803198520212 1105 46-47 -0.9049863703660819 1105 48-49 -0.8117033727493137 1105 50-51 -0.8079155764792034 1105 52-53 -0.7108812745626665 1105 54-55 -0.5893863118340121 1105 56-57 -0.46269263194643173 1105 58-59 -0.435788496556782 1105 60-61 -0.4720514103860083 1105 62-63 -0.5755129196987809 1105 64-65 -0.6335629653728816 1105 66-67 -0.6037766517763004 1105 68-69 -0.6102741229035757 1105 70-71 -0.676470410393371 1105 72-73 -0.48071399430760664 1105 74-75 -0.5247151932896301 1105 76-77 -0.4789800737644043 1105 78-79 -0.6073847128936478 1105 80-81 -0.6424289450642249 1105 82-83 -0.6497442346860893 1105 84-85 -0.6996332450095579 1105 86-87 -0.7379771461673847 1105 88-89 -0.7519222044810832 1105 90-91 -0.8800456160731365 1105 92-93 -0.785460559814009 1105 94-95 -0.857416134491082 1105 96-97 -0.8563695480566018 1105 98-99 -0.8656266986120542 1105 100 -0.8293650343692036 1106 1 -0.5204306784954689 1106 2 -0.43722686523148013 1106 3 -0.5608570265991055 1106 4 -0.49562814208456984 1106 5 -0.40440406210288415 1106 6 -0.3560665895190809 1106 7 -0.3933159136064788 1106 8 -0.49953328258805385 1106 9 -0.5926958978753447 1106 10-11 -0.5660720049109571 1106 12-13 -0.5897945699302269 1106 14-15 -0.813163100930332 1106 16-17 -0.8396578225194631 1106 18-19 -0.8251905881808952 1106 20-21 -0.7894560454048971 1106 22-23 -0.7379417010124243 1106 24-25 -0.7155102910203084 1106 26-27 -0.7960632831520655 1106 28-29 -0.9327505829031821 1106 30-31 -0.8954582112531071 1106 32-33 -0.9337086862268151 1106 34-35 -0.9392953962952859 1106 36-37 -0.9836512986343422 1106 38-39 -0.934627872083567 1106 40-41 -0.9667523986492412 1106 42-43 -0.9266679793410759 1106 44-45 -0.9799128716017691 1106 46-47 -0.8623609642153909 1106 48-49 -0.9796236034686103 1106 50-51 -1.0752066104462088 1106 52-53 -0.8971994164714516 1106 54-55 -0.8509473150666338 1106 56-57 -0.7604035339056594 1106 58-59 -0.8093416922445158 1106 60-61 -0.8693430412981513 1106 62-63 -0.9107781789014808 1106 64-65 -0.9827381032713518 1106 66-67 -0.8703368217730088 1106 68-69 -0.9189181371606168 1106 70-71 -0.876817716388274 1106 72-73 -0.8864360243190177 1106 74-75 -0.8660060539767329 1106 76-77 -0.8234998065319701 1106 78-79 -0.8783922484968656 1106 80-81 -0.7326923139406674 1106 82-83 -0.7528862595405599 1106 84-85 -0.818207897558473 1106 86-87 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24-25 0.8848001082953729 2311 26-27 0.8695625568964047 2311 28-29 0.8715638459419566 2311 30-31 0.8914152571367353 2311 32-33 0.9223050771272341 2311 34-35 0.8890039987046308 2311 36-37 0.9182288931467468 2311 38-39 0.9171295205681957 2311 40-41 0.9586853726629485 2311 42-43 0.9523910222090421 2311 44-45 1.0652704500628687 2311 46-47 0.987422076139076 2311 48-49 1.0019660182704229 2311 50-51 0.9754511615808141 2311 52-53 0.9116915138134232 2311 54-55 0.8134149569320144 2311 56-57 0.7269549887924356 2311 58-59 0.7307928987248999 2311 60-61 0.7261435613273548 2311 62-63 0.7174255087667376 2311 64-65 0.7610262629652951 2311 66-67 0.7678696111172769 2311 68-69 0.7583068818441916 2311 70-71 0.7678591959036751 2311 72-73 0.7745483780740656 2311 74-75 0.7832778305185002 2311 76-77 0.7574632197720064 2311 78-79 0.8205265875409893 2311 80-81 0.8282876882879506 2311 82-83 0.92863875637061 2311 84-85 0.8781983244242682 2311 86-87 0.8668162426203203 2311 88-89 0.9344650716206218 2311 90-91 0.9621958248454376 2311 92-93 1.0081704166723817 2311 94-95 1.028950766712498 2311 96-97 1.0852052059828274 2311 98-99 1.2009000507958305 2311 100 1.2624640359362864 2312 1 0.5601388583882745 2312 2 0.5165378982753701 2312 3 0.5847430845127484 2312 4 0.23772543370014176 2312 5 0.31372970208438034 2312 6 0.4243450710194381 2312 7 0.25844048843816125 2312 8 0.4326053028487564 2312 9 0.5706896447294767 2312 10-11 0.5749449640257538 2312 12-13 0.6825197406702372 2312 14-15 0.8763960051807516 2312 16-17 0.8386609606284665 2312 18-19 0.8567312774343492 2312 20-21 0.8273283451532549 2312 22-23 0.8616188752620886 2312 24-25 0.8786864789050242 2312 26-27 0.886221247404066 2312 28-29 0.9693912753095333 2312 30-31 0.9593632229910654 2312 32-33 1.0215138292893187 2312 34-35 0.952431572929548 2312 36-37 0.9990481282974315 2312 38-39 1.0140007757132992 2312 40-41 1.039508724540859 2312 42-43 1.0603388787127983 2312 44-45 1.156814692337413 2312 46-47 1.1992911153270995 2312 48-49 1.1316034954113334 2312 50-51 1.097781094111511 2312 52-53 1.0328991879075744 2312 54-55 1.001349279695006 2312 56-57 0.9363749612232226 2312 58-59 0.8876559976041847 2312 60-61 0.833179434102469 2312 62-63 0.8386703620536622 2312 64-65 0.8675346157405173 2312 66-67 0.8145722090937753 2312 68-69 0.9025488347681261 2312 70-71 0.8833644952251305 2312 72-73 0.8938537382458875 2312 74-75 0.8977366127519915 2312 76-77 0.9102254472559252 2312 78-79 0.9352747306718854 2312 80-81 0.8887341730853251 2312 82-83 0.9163121172216933 2312 84-85 1.0073578752635939 2312 86-87 1.0784008095245348 2312 88-89 1.0174641781621716 2312 90-91 1.045517290426055 2312 92-93 1.1438689062837604 2312 94-95 1.2038888710753923 2312 96-97 1.1597006978448938 2312 98-99 0.7387624273181359 2312 100 1.118533732128249 2313 1 0.5542966621910175 2313 2 0.5287287855060008 2313 3 0.5492801706184558 2313 4 0.42135442570221215 2313 5 0.30324258539694426 2313 6 0.37918417085059275 2313 7 0.4294619603605341 2313 8 0.45386811308878094 2313 9 0.5862398000782534 2313 10-11 0.6285055728592823 2313 12-13 0.6961139390021174 2313 14-15 0.8712006667580638 2313 16-17 0.862749277880539 2313 18-19 0.8388286371151779 2313 20-21 0.8617426746921169 2313 22-23 0.9205285533556591 2313 24-25 0.8682876858353197 2313 26-27 0.893306923435162 2313 28-29 0.9348578547665767 2313 30-31 0.9417207262282616 2313 32-33 0.9610519896011951 2313 34-35 0.9813237931837122 2313 36-37 1.064928688242638 2313 38-39 1.109232732630474 2313 40-41 1.1278465058834044 2313 42-43 1.162027424704469 2313 44-45 1.1379361423512862 2313 46-47 1.1763868650248597 2313 48-49 1.1538041274158886 2313 50-51 1.1309755498284915 2313 52-53 1.0770065703297433 2313 54-55 0.9715423132160694 2313 56-57 0.92799976037319 2313 58-59 0.9305470073208539 2313 60-61 0.932511569629618 2313 62-63 0.9137537655419408 2313 64-65 0.9086937398885127 2313 66-67 0.9042595116586654 2313 68-69 0.9102709182718769 2313 70-71 0.9441916711026508 2313 72-73 0.9343594267642956 2313 74-75 0.8948684027295712 2313 76-77 0.7147346199156033 2313 78-79 0.8589526215993786 2313 80-81 0.8646499404690289 2313 82-83 0.9134574342398238 2313 84-85 0.9476528602326653 2313 86-87 0.9569819343695691 2313 88-89 1.0213429376848069 2313 90-91 1.0870623330839777 2313 92-93 1.0754486577363274 2313 94-95 1.1050178219658697 2313 96-97 1.1106870843373748 2313 98-99 1.1771481898692393 2313 100 1.2471425741446147 2314 1 0.4284257160785536 2314 2 0.4971909632838809 2314 3 0.5059829437346046 2314 4 0.32057906372583034 2314 5 0.3272295701166996 2314 6 0.3472392405075695 2314 7 0.4061919452068423 2314 8 0.3623597735889845 2314 9 0.5716645342912869 2314 10-11 0.5065308056121935 2314 12-13 0.5756401093850982 2314 14-15 0.7173479888110776 2314 16-17 0.7027757791327076 2314 18-19 0.6829761962056438 2314 20-21 0.7321279324428573 2314 22-23 0.7414796296505344 2314 24-25 0.7705967181780338 2314 26-27 0.8121727785287334 2314 28-29 0.8246856389178561 2314 30-31 0.8392818413446292 2314 32-33 0.8392322799477512 2314 34-35 0.8105064971466689 2314 36-37 0.8545450946174498 2314 38-39 0.8447841528939577 2314 40-41 0.947135983482255 2314 42-43 1.039940143962788 2314 44-45 1.0203454789625468 2314 46-47 1.0724808325063222 2314 48-49 1.0157433136711873 2314 50-51 0.9752120349669724 2314 52-53 0.9445146142199405 2314 54-55 0.8723461260300738 2314 56-57 0.8273138742194703 2314 58-59 0.8021221465539128 2314 60-61 0.7513648327694185 2314 62-63 0.8189761171374101 2314 64-65 0.8729130318823835 2314 66-67 0.8874125982045058 2314 68-69 0.8649001740430009 2314 70-71 0.8495365590072979 2314 72-73 0.8929770755240654 2314 74-75 0.8457001007476634 2314 76-77 0.8194135827149935 2314 78-79 0.8782121893432944 2314 80-81 0.9059022065713762 2314 82-83 0.9482015633074568 2314 84-85 0.9745468312242309 2314 86-87 0.9606800569208893 2314 88-89 1.0355521698600256 2314 90-91 1.062505929853458 2314 92-93 1.138094114097381 2314 94-95 1.1634725154078325 2314 96-97 1.172480971498878 2314 98-99 1.277487231660679 2314 100 1.278036335376008 2315 1 0.3535189585158669 2315 2 0.36477019663304944 2315 3 0.4064345548002599 2315 4 0.10909360895188769 2315 5 0.1579966524413834 2315 6 0.28086280338550296 2315 7 0.3443144586717324 2315 8 0.3228213113186058 2315 9 0.4643090408263788 2315 10-11 0.3148723569776237 2315 12-13 0.3662706823176549 2315 14-15 0.5774226002118468 2315 16-17 0.5776858939196572 2315 18-19 0.4794654994264178 2315 20-21 0.5414110958821539 2315 22-23 0.3976192584358458 2315 24-25 0.5493902917444515 2315 26-27 0.6166212793421622 2315 28-29 0.6641711792013041 2315 30-31 0.6978645758258111 2315 32-33 0.7226858760761203 2315 34-35 0.7833849448742072 2315 36-37 0.7927215189546359 2315 38-39 0.7801743615535486 2315 40-41 0.8458238053153622 2315 42-43 0.916041605928001 2315 44-45 0.892850078162418 2315 46-47 0.9418522128301205 2315 48-49 0.9280654132459958 2315 50-51 0.8691076476877271 2315 52-53 0.8917802246531608 2315 54-55 0.8154419745958208 2315 56-57 0.7602694512659696 2315 58-59 0.7170291322250364 2315 60-61 0.7048776745573448 2315 62-63 0.7774081130801562 2315 64-65 0.8037402824800424 2315 66-67 0.7739751585101544 2315 68-69 0.7645686128296347 2315 70-71 0.7340725275622404 2315 72-73 0.481375491121959 2315 74-75 0.7065815158791793 2315 76-77 0.7654574211250562 2315 78-79 0.7485546093905029 2315 80-81 0.4732297914476007 2315 82-83 0.4931181492688168 2315 84-85 0.8255322253666151 2315 86-87 0.917629316901202 2315 88-89 0.9053854178188985 2315 90-91 1.0541873887701598 2315 92-93 1.1098535528575688 2315 94-95 1.0535527549374706 2315 96-97 0.888927234107399 2315 98-99 0.2279021407446109 2315 100 0.8022826307382438 2316 1 0.2469296505239882 2316 2 0.23155005112603888 2316 3 0.24516149091091322 2316 4 0.149670993905346 2316 5 0.057534547362962485 2316 6 0.12375678907330467 2316 7 0.10498924990483971 2316 8 0.19068784270901773 2316 9 0.24202120217599088 2316 10-11 0.19016736197057327 2316 12-13 0.010691494739461405 2316 14-15 -0.03471230680928983 2316 16-17 0.1949559506682661 2316 18-19 0.20364501318798744 2316 20-21 0.24364551045223948 2316 22-23 0.26722769187145445 2316 24-25 0.3076000000593808 2316 26-27 0.3337580986105593 2316 28-29 0.32151744102682756 2316 30-31 0.16547152720576008 2316 32-33 0.3764151680467265 2316 34-35 0.35367329414019366 2316 36-37 0.381366857994081 2316 38-39 0.5728746893044274 2316 40-41 0.5535677766651546 2316 42-43 0.4752319134110081 2316 44-45 0.3770389641382863 2316 46-47 0.30312249005321235 2316 48-49 0.32162633934944296 2316 50-51 0.3283809302074303 2316 52-53 0.46805334950083477 2316 54-55 0.15468062254223724 2316 56-57 0.033660435806432076 2316 58-59 0.06641875498725369 2316 60-61 0.18345989048361133 2316 62-63 0.30693050614618755 2316 64-65 0.22767699769925542 2316 66-67 0.1877729573921414 2316 68-69 0.17834141367612943 2316 70-71 -0.05654786118385147 2316 72-73 -0.05486636364211961 2316 74-75 0.1379791099412131 2316 76-77 0.29900548143629635 2316 78-79 0.29091705898078857 2316 80-81 0.4731124338669943 2316 82-83 0.4374571443498425 2316 84-85 0.4853796430951647 2316 86-87 0.5012183114955882 2316 88-89 0.5302193063852378 2316 90-91 0.4200886750113 2316 92-93 0.4727782287564999 2316 94-95 0.6698870143250808 2316 96-97 0.5851103414390266 2316 98-99 0.5514317158282083 2316 100 0.3867731938261656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 24.0 1 307.0 2 137.0 3 122.0 4 86.0 5 75.0 6 106.0 7 718.0 8 2407.0 9 3713.0 10 4793.0 11 5639.0 12 7263.0 13 8441.0 14 9325.0 15 10813.0 16 12799.0 17 16878.0 18 21462.0 19 26614.0 20 33005.0 21 40323.0 22 45429.0 23 45944.0 24 45029.0 25 45178.0 26 49474.0 27 57844.0 28 70150.0 29 85766.0 30 107181.0 31 137663.0 32 182245.0 33 247522.0 34 361598.0 35 519390.0 36 800473.0 37 1174741.0 38 1084325.0 39 295808.0 40 380.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.43154049845974 19.024742351878192 12.434290328349551 24.109426821312514 2 15.939038771413397 19.677694059610936 40.85207089938517 23.531196269590502 3 19.525252405354962 25.340422156926007 28.901631602024498 26.232693835694537 4 12.657872710524224 15.793259901293698 38.0288126576569 33.52005473052518 5 13.347093050024172 37.26864108289786 34.603859003430095 14.780406863647874 6 31.182468383523 36.484951195619615 18.13308290223022 14.199497518627163 7 27.85616629732855 31.693150641554436 22.36801837161022 18.082664689506796 8 26.17019397195726 35.397431037779924 20.05396335435606 18.37841163590675 9 26.553667031595552 15.628736008910838 20.810965999013504 37.00663096048011 10-11 25.648455576317264 25.774743720333053 27.834434003629177 20.742366699720506 12-13 25.67369497345227 23.439704740599282 28.206684458694696 22.67991582725375 14-15 22.90181616423488 24.93059836346036 26.000972086023765 26.166613386280996 16-17 22.234713623143627 27.61296012720247 26.946032022368698 23.206294227285206 18-19 21.877925609234868 27.169589769280233 28.96968107773835 21.98280354374654 20-21 22.82618421408788 26.5752329031335 28.67622388520474 21.92235899757387 22-23 22.76433716736962 26.403023462863207 28.46399633083626 22.36864303893091 24-25 22.36835620848614 26.276410144805023 28.98737205232478 22.36786159438406 26-27 22.351517178927566 26.557613951409625 28.418479340953624 22.672389528709182 28-29 22.02528032649109 26.984173088333126 28.587978554058992 22.402568031116793 30-31 22.677316424935714 26.63773125042938 28.633634719190322 22.051317605444584 32-33 22.668632861496935 26.716220819578783 28.21894259293832 22.396203725985966 34-35 22.717094842670196 26.778859310241504 28.5221900407386 21.981855806349703 36-37 22.57411546295371 26.629057259710574 28.254330766796464 22.542496510539245 38-39 22.582125768731224 26.82744386768151 28.436357952966105 22.154072410621158 40-41 22.55427309636106 26.258950040567342 28.713179808740858 22.47359705433074 42-43 22.439082782805396 26.5110768714952 28.293283655112177 22.75655669058723 44-45 22.344267949744808 26.99142071162627 28.313639391062555 22.35067194756637 46-47 22.364739441566243 26.75342907979114 27.888604681975345 22.99322679666727 48-49 22.66521630964487 26.755822129912964 27.913160622842838 22.665800937599332 50-51 22.532049425741775 26.82888662577473 27.881210728504126 22.757853219979374 52-53 22.409009993034807 27.13460088302508 27.633955811329663 22.82243331261045 54-55 22.27180914873398 26.971624465913724 27.810719806103723 22.94584657924857 56-57 22.73091373255715 26.82543962624529 27.50756679883306 22.936079842364503 58-59 22.297502167927515 27.040597472818494 27.908820849809597 22.753079509444394 60-61 22.46806568932805 27.297416927446378 27.60093699038243 22.633580392843147 62-63 22.603816066343285 27.009282153397717 27.455898215208475 22.931003565050524 64-65 22.21978809613419 26.97019199769058 27.61149728881221 23.198522617363025 66-67 22.349281399007786 26.824445726338226 27.536418404373215 23.28985447028078 68-69 22.1590197217525 26.861674307720357 27.7197244777519 23.259581492775244 70-71 22.214169223778494 26.666332074475186 27.708289935286988 23.41120876645933 72-73 22.482066625596943 26.814580633241885 27.65296605523509 23.05038668592608 74-75 22.61398837801707 26.955176270038656 27.407716841982673 23.023118509961606 76-77 22.47971565861408 26.765456665438265 27.35338917068954 23.40143850525811 78-79 22.336728535806454 26.826841616398983 27.4581285988803 23.37830124891426 80-81 22.246029681924902 27.065754273202202 27.488982679848238 23.199233365024654 82-83 22.6714296492609 26.716310844325413 27.945209273607684 22.667050232806 84-85 22.777005120102235 26.908677018131442 27.325084904173313 22.98923295759301 86-87 22.180749591645608 27.14661777354349 27.65161491629915 23.021017718511754 88-89 22.557903263574726 26.541251410465268 27.596344174746978 23.304501151213028 90-91 22.651887496172492 26.577498245303232 27.542853494398233 23.22776076412604 92-93 22.745370320827842 26.658668054883233 27.533197731024888 23.062763893264034 94-95 22.18704903471678 26.62216205779604 27.821273884101622 23.36951502338556 96-97 22.372780176962628 26.578153877394172 27.58261000315744 23.46645594248575 98-99 22.121951745944955 26.772403026040788 27.372655915564398 23.732989312449863 100 22.559400568630974 26.475874732830317 27.09485043084399 23.869874267694712 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1456.0 1 1034.0 2 1613.0 3 2307.5 4 1508.0 5 1094.0 6 1531.5 7 2716.5 8 4155.0 9 4729.0 10 4148.0 11 3579.5 12 3770.5 13 4175.0 14 4637.5 15 5209.0 16 5796.5 17 6345.0 18 6945.0 19 7590.0 20 8207.5 21 9017.5 22 10226.5 23 12187.0 24 14882.5 25 18144.5 26 22614.5 27 27847.5 28 34103.0 29 43221.5 30 54608.5 31 65606.0 32 79068.5 33 93467.5 34 106008.0 35 118490.5 36 130571.5 37 147237.5 38 158193.5 39 160090.5 40 160043.0 41 158735.0 42 162412.0 43 170509.5 44 180992.0 45 198470.5 46 214690.5 47 224967.0 48 232840.5 49 234657.0 50 231138.5 51 233008.5 52 230097.5 53 219012.0 54 207360.5 55 186398.0 56 166476.5 57 144729.5 58 123014.0 59 109469.5 60 90274.5 61 69406.5 62 55007.5 63 44059.5 64 33069.0 65 22416.5 66 15862.5 67 11929.0 68 9342.5 69 7337.5 70 5917.0 71 4633.0 72 3607.0 73 3023.5 74 2426.0 75 1822.0 76 1316.5 77 928.5 78 670.0 79 457.5 80 315.0 81 222.5 82 155.0 83 112.5 84 88.5 85 69.5 86 59.0 87 47.0 88 42.0 89 37.5 90 26.0 91 19.0 92 15.0 93 15.5 94 13.0 95 6.5 96 4.0 97 3.5 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0544487780493024 2 0.12299525820912431 3 0.015967805451710875 4 0.01539238903903661 5 0.017945799370278664 6 0.049899392036596484 7 0.05482639507011989 8 0.046806528818472304 9 0.038049410288085825 10-11 0.037357112416587096 12-13 0.038588863174967944 14-15 0.017667082045389568 16-17 0.016552212745833177 18-19 0.016480285694248892 20-21 0.01977094830422985 22-23 0.02409556228073488 24-25 0.023376291764892046 26-27 0.018611124597433283 28-29 0.017568182349461177 30-31 0.015752024296958024 32-33 0.021793896630037817 34-35 0.022108577480719052 36-37 0.022180504532303337 38-39 0.0204182917684884 40-41 0.023358310001995976 42-43 0.03017339813960681 44-45 0.038885562262753116 46-47 0.043992382925237224 48-49 0.03774372031885262 50-51 0.026900717292521928 52-53 0.038085373813877964 54-55 0.034686820626520586 56-57 0.03661086925640016 58-59 0.04152888140847553 60-61 0.022675003011945284 62-63 0.024832814559473785 64-65 0.024410243131416116 66-67 0.030694869263592862 68-69 0.037303167127898884 70-71 0.03927217016501864 72-73 0.04226613368721443 74-75 0.026388237049983904 76-77 0.020013702103326807 78-79 0.018710024293361674 80-81 0.023520145868060612 82-83 0.01957314891237307 84-85 0.011805027341270483 86-87 0.013711094208253987 88-89 0.017406346483396538 90-91 0.02000471122187877 92-93 0.019033696025490945 94-95 0.022324358635471903 96-97 0.023655009089781145 98-99 0.026073556199302666 100 0.027350261364923696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5561190.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.15947931254972 #Duplication Level Percentage of deduplicated Percentage of total 1 80.43470174098739 56.432567928079116 2 12.786434369311998 17.941791552300394 3 3.5267403822628003 7.423028066703019 4 1.3425408624786972 3.767678714693073 5 0.6418108821711309 2.2514558655127375 6 0.3428670832933766 1.4433225622365549 7 0.20920028383453268 1.0274168090107898 8 0.14966445169962436 0.8400304002275113 9 0.10666988814836624 0.6735513429735565 >10 0.43315931307269084 5.060316868472256 >50 0.017586957497417663 0.8260853362160062 >100 0.007884383771140842 0.9777992569092312 >500 4.140652888347059E-4 0.1991002891369172 >1k 2.3660813236173745E-4 0.347589602134841 >5k 2.9576016544571546E-5 0.1797812854673388 >10k+ 5.915203308914309E-5 0.6084841199266209 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16573 0.29801175647658146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12751 0.22928545868779884 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8664 0.15579399373155745 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7981762896070806E-5 0.0 5 0.0 3.596352579214161E-5 0.0 1.7981762896070806E-5 0.0 6 0.0 3.596352579214161E-5 0.0 1.7981762896070806E-5 1.7981762896070806E-5 7 0.0 5.3945288688212413E-5 0.0 1.7981762896070806E-5 1.7981762896070806E-5 8 0.0 5.3945288688212413E-5 0.0 1.7981762896070806E-5 1.7981762896070806E-5 9 0.0 5.3945288688212413E-5 0.0 7.192705158428322E-5 1.7981762896070806E-5 10-11 0.0 5.3945288688212413E-5 0.0 7.192705158428322E-5 3.596352579214161E-5 12-13 0.0 5.3945288688212413E-5 0.0 7.192705158428322E-5 6.293617013624782E-5 14-15 0.0 6.293617013624782E-5 0.0 8.990881448035403E-5 1.2587234027249564E-4 16-17 0.0 7.192705158428322E-5 0.0 8.990881448035403E-5 2.1578115475284965E-4 18-19 0.0 1.2587234027249564E-4 0.0 1.0789057737642483E-4 2.3376291764892046E-4 20-21 0.0 1.2587234027249564E-4 0.0 1.3486322172053103E-4 2.3376291764892046E-4 22-23 0.0 1.2587234027249564E-4 0.0 3.0568996923320367E-4 2.787173248890975E-4 24-25 0.0 1.2587234027249564E-4 0.0 8.451428561153278E-4 3.326626135773099E-4 26-27 0.0 1.2587234027249564E-4 0.0 0.0015644133719581602 3.596352579214161E-4 28-29 0.0 1.2587234027249564E-4 0.0 0.0036952522751425507 3.596352579214161E-4 30-31 0.0 1.2587234027249564E-4 0.0 0.013585221867981494 3.686261393694515E-4 32-33 0.0 1.2587234027249564E-4 0.0 0.03742004858672335 3.776170208174869E-4 34-35 0.0 1.2587234027249564E-4 0.0 0.07252044975985356 3.955987837135577E-4 36-37 0.0 1.2587234027249564E-4 0.0 0.12841675972228966 3.955987837135577E-4 38-39 0.0 1.4385410316856644E-4 0.0 0.21254443743155693 4.405531909537347E-4 40-41 0.0 1.7981762896070806E-4 0.0 0.3074971364042588 4.4954407240177014E-4 42-43 0.0 1.7981762896070806E-4 0.0 0.3959494280900311 4.5853495384980553E-4 44-45 0.0 1.8880851040874345E-4 0.0 0.4988770389071404 4.765167167458763E-4 46-47 0.0 1.9779939185677884E-4 0.0 0.6227534034981721 4.855075981939117E-4 48-49 0.0 1.9779939185677884E-4 0.0 0.7593248926938299 5.034893610899825E-4 50-51 0.0 1.9779939185677884E-4 0.0 0.8988274092415472 5.034893610899825E-4 52-53 0.0 1.9779939185677884E-4 0.0 1.0346886188028104 5.214711239860533E-4 54-55 0.0 2.6073556199302666E-4 0.0 1.1921639073651504 5.394528868821241E-4 56-57 0.0 2.6972644344106205E-4 0.0 1.3706598767529972 5.394528868821241E-4 58-59 0.0 3.236717321292745E-4 0.0 1.5702034996106948 5.394528868821241E-4 60-61 0.0 3.236717321292745E-4 0.0 1.7715363078765516 5.57434649778195E-4 62-63 0.0 3.236717321292745E-4 0.0 1.9789649337641761 5.57434649778195E-4 64-65 0.0 3.416534950253453E-4 0.0 2.1932086477894117 5.57434649778195E-4 66-67 0.0 3.596352579214161E-4 0.0 2.432159304033849 5.664255312262304E-4 68-69 0.0 3.596352579214161E-4 0.0 2.6967699359309787 5.754164126742658E-4 70-71 0.0 3.776170208174869E-4 0.0 2.974435327690656 6.293617013624781E-4 72-73 0.0 3.955987837135577E-4 0.0 3.2523434732494305 6.653252271546198E-4 74-75 0.0 3.955987837135577E-4 0.0 3.529091795101408 6.653252271546198E-4 76-77 0.0 3.955987837135577E-4 0.0 3.841102353992581 6.653252271546198E-4 78-79 0.0 3.955987837135577E-4 0.0 4.177190493401592 6.653252271546198E-4 80-81 0.0 3.955987837135577E-4 0.0 4.528446249813439 6.653252271546198E-4 82-83 0.0 3.955987837135577E-4 0.0 4.8911294165457395 6.833069900506906E-4 84-85 0.0 3.955987837135577E-4 0.0 5.253021385710612 6.833069900506906E-4 86-87 0.0 4.135805466096285E-4 0.0 5.6311598776520855 6.833069900506906E-4 88 0.0 4.135805466096285E-4 0.0 5.934017719229158 6.833069900506906E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7620 0.0 46.93182 1 GTATCAA 13805 0.0 42.027683 1 CAACGCA 16320 0.0 34.50672 5 TACCTGG 4370 0.0 34.456676 2 ATCAACG 16325 0.0 34.41008 3 TATCAAC 17625 0.0 32.784916 2 AACGCAG 17935 0.0 31.976387 6 TCAACGC 18200 0.0 31.278059 4 GTACTAG 3085 0.0 29.895763 1 GTACATG 17750 0.0 29.558695 1 CTAACGC 1370 0.0 29.165493 3 GTACCTG 5360 0.0 28.970629 1 TACATGG 17860 0.0 28.849447 2 ACGCAGA 19560 0.0 28.83921 7 CGCAGAG 20085 0.0 28.106007 8 TAACGCC 1440 0.0 28.073917 4 ACATGGG 17845 0.0 28.028252 3 TCTAACG 1490 0.0 26.843397 2 ACCTGGG 5535 0.0 26.837399 3 CATGGGG 14080 0.0 26.174623 4 >>END_MODULE