FastQCFastQC Report
Mon 6 Jun 2016
ERR1211187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5561190
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148300.26666954374873003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126980.22833242525430708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86860.156189592515271No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84400.048.4157941
GTATCAA147450.038.0456851
TACCTGG45500.034.1906622
ATCAACG172400.031.5176963
CAACGCA173650.031.3345265
GTACTAG30900.031.1964451
GTACCTG54250.029.9039861
TATCAAC187400.029.7444552
TCAACGC189650.028.824434
AACGCAG193300.028.6596786
ACCTGGG55350.027.2597123
GTACATG180700.027.1936721
TACATGG182000.026.3918882
ACGCAGA212150.025.7366777
CTGTGCG40600.025.691939
ACATGGG183450.025.3915863
TAGTACT40900.025.1683484
CGCAGAG216600.025.054948
GTGGTAT37250.024.742271
CTAGTAC43450.024.123983
Produced by FastQC (version 0.11.5)