##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211187_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5561190 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89830809592911 31.0 31.0 34.0 30.0 34.0 2 32.04652835094647 33.0 31.0 34.0 30.0 34.0 3 32.119022367514866 33.0 31.0 34.0 30.0 34.0 4 35.79677928644769 37.0 35.0 37.0 35.0 37.0 5 35.666452144235315 37.0 35.0 37.0 33.0 37.0 6 35.69573490565868 37.0 35.0 37.0 33.0 37.0 7 35.62644326124445 37.0 35.0 37.0 33.0 37.0 8 35.6084852342754 37.0 35.0 37.0 33.0 37.0 9 37.17176521571822 39.0 37.0 39.0 33.0 39.0 10-11 37.133903714852394 39.0 37.0 39.0 33.0 39.0 12-13 37.02591846349432 39.0 37.0 39.0 33.0 39.0 14-15 38.177700276379696 40.0 38.0 41.0 33.0 41.0 16-17 38.120381519063365 40.0 37.0 41.0 33.0 41.0 18-19 38.133226791388175 40.0 37.0 41.0 33.0 41.0 20-21 38.088345300196536 40.0 37.0 41.0 33.0 41.0 22-23 37.94864947969769 40.0 37.0 41.0 33.0 41.0 24-25 37.873353275108386 40.0 37.0 41.0 33.0 41.0 26-27 37.71712852824665 40.0 37.0 41.0 32.5 41.0 28-29 37.6797523551614 40.0 37.0 41.0 32.5 41.0 30-31 37.68864163964906 40.0 37.0 41.0 33.0 41.0 32-33 37.54484966347131 40.0 36.0 41.0 32.0 41.0 34-35 37.382176206890975 39.0 36.0 41.0 32.0 41.0 36-37 37.21698368514652 39.0 36.0 41.0 31.0 41.0 38-39 37.11709265462967 39.0 36.0 41.0 31.0 41.0 40-41 37.0141075021713 39.0 36.0 41.0 31.0 41.0 42-43 36.86913915906487 39.0 35.0 40.0 31.0 41.0 44-45 36.727727159115226 39.0 35.0 40.0 30.0 41.0 46-47 36.490235003659286 39.0 35.0 40.0 30.0 41.0 48-49 36.25814358078037 39.0 35.0 40.0 29.5 41.0 50-51 36.06046826308757 38.5 35.0 40.0 29.0 41.0 52-53 35.853965697989096 38.0 35.0 40.0 28.0 41.0 54-55 35.605643216649675 38.0 34.0 40.0 28.0 41.0 56-57 35.34033956761053 38.0 34.0 40.0 27.0 41.0 58-59 35.06342122819037 37.0 34.0 40.0 27.0 41.0 60-61 34.79354607557016 37.0 33.5 40.0 26.0 41.0 62-63 34.483082038196855 36.5 33.0 39.0 26.0 41.0 64-65 34.096702953864195 36.0 33.0 39.0 26.0 40.0 66-67 33.78548494117266 35.5 32.0 39.0 25.5 40.0 68-69 33.45238213403965 35.0 32.0 38.0 25.0 40.0 70-71 33.11275068825198 35.0 32.0 38.0 25.0 40.0 72-73 32.761186634515276 35.0 31.0 37.0 24.0 39.0 74-75 32.411687426611934 35.0 31.0 36.5 24.0 39.0 76-77 31.48108390470385 34.0 30.0 35.5 22.5 38.0 78-79 32.013507990196345 34.0 31.0 36.0 24.0 38.0 80-81 31.946536173013328 34.0 31.0 35.0 24.0 37.0 82-83 31.691549110891735 34.0 31.0 35.0 24.0 37.0 84-85 31.428747354433135 34.0 31.0 35.0 23.0 36.0 86-87 31.17059756994456 34.0 30.5 35.0 22.5 36.0 88-89 30.937486671018252 34.0 30.0 35.0 20.5 36.0 90-91 30.706066866983505 34.0 30.0 35.0 20.0 35.0 92-93 30.505359284613547 34.0 29.5 35.0 20.0 35.0 94-95 30.29212704475121 34.0 29.0 35.0 19.5 35.0 96-97 30.091371361165507 33.5 29.0 35.0 19.0 35.0 98-99 29.884510869076585 33.0 29.0 35.0 18.5 35.0 100 28.081893084034174 31.0 25.0 34.0 14.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4195549272184991 1101 2 -0.42273801356873975 1101 3 -0.48249779836066153 1101 4 -0.27845851783919073 1101 5 -0.30912289618424893 1101 6 -0.45671171778966624 1101 7 -0.34187760110192755 1101 8 -0.39485504223044643 1101 9 -0.5580653095889403 1101 10-11 -0.6006832251490763 1101 12-13 -0.5879765387313896 1101 14-15 -0.8543657511811702 1101 16-17 -0.8047961443536096 1101 18-19 -0.8814121806099919 1101 20-21 -0.8359037730181598 1101 22-23 -0.9512847722785267 1101 24-25 -0.8843981459067862 1101 26-27 -1.0254111555170482 1101 28-29 -1.0830295193233965 1101 30-31 -0.8920057411048177 1101 32-33 -0.9126567829548904 1101 34-35 -1.0159309859006953 1101 36-37 -0.9565777353810034 1101 38-39 -0.8756451129622178 1101 40-41 -0.9690959577784852 1101 42-43 -0.9713091063139885 1101 44-45 -1.0234304745763012 1101 46-47 -1.0377597962278813 1101 48-49 -1.0411881093572504 1101 50-51 -1.1553264127755227 1101 52-53 -1.0671827479555418 1101 54-55 -1.0396989101534757 1101 56-57 -1.1123863627624075 1101 58-59 -1.1295649022717669 1101 60-61 -1.2396658403535596 1101 62-63 -1.4057606686290924 1101 64-65 -1.2759558111950398 1101 66-67 -1.2189359392865384 1101 68-69 -1.3363687216113576 1101 70-71 -1.403100118118985 1101 72-73 -1.423307977284022 1101 74-75 -1.5483373637463416 1101 76-77 -1.358504566510792 1101 78-79 -1.3009752122701315 1101 80-81 -1.2495010560069524 1101 82-83 -1.2939296923822603 1101 84-85 -1.1478253509480147 1101 86-87 -1.2351037705644217 1101 88-89 -1.3749303532018402 1101 90-91 -1.3496180926773853 1101 92-93 -1.3417240680035682 1101 94-95 -1.4524590856694282 1101 96-97 -1.5216706797331376 1101 98-99 -1.5847135569390929 1101 100 -1.2034930809744324 1102 1 -0.5501766806620658 1102 2 -0.47306106050566754 1102 3 -0.5692393514852796 1102 4 -0.39820867870984955 1102 5 -0.4831792615331736 1102 6 -0.47317237675319035 1102 7 -0.4792881896553993 1102 8 -0.6839425092403033 1102 9 -0.7404001257300905 1102 10-11 -0.703889960267368 1102 12-13 -0.7584773360903156 1102 14-15 -0.9219258328754876 1102 16-17 -0.9549496009744871 1102 18-19 -0.8770954379377685 1102 20-21 -0.9872248242263169 1102 22-23 -0.9351625478802674 1102 24-25 -1.0293594903192087 1102 26-27 -1.0682268117294456 1102 28-29 -1.0640827325399727 1102 30-31 -1.0518027176689912 1102 32-33 -0.9389229049927721 1102 34-35 -0.9403304953617564 1102 36-37 -1.1088819161529955 1102 38-39 -1.0806735567600683 1102 40-41 -1.0648001516066543 1102 42-43 -1.0912237282912542 1102 44-45 -1.0888046270910152 1102 46-47 -1.1809603762948413 1102 48-49 -1.1294663736327664 1102 50-51 -1.1667352200944379 1102 52-53 -1.2296414111903005 1102 54-55 -1.2173483333505573 1102 56-57 -1.2607978465960699 1102 58-59 -1.4380923062389783 1102 60-61 -1.382836997409946 1102 62-63 -1.3013644232104085 1102 64-65 -1.2462721435305113 1102 66-67 -1.4138789118033017 1102 68-69 -1.341855110554647 1102 70-71 -1.4482938623486277 1102 72-73 -1.3295374021597546 1102 74-75 -1.5054042020083784 1102 76-77 -1.17483312501642 1102 78-79 -1.068600341666098 1102 80-81 -1.1328183489034593 1102 82-83 -1.0639043261100802 1102 84-85 -1.1410588512138027 1102 86-87 -1.1289814995369944 1102 88-89 -1.183712072699361 1102 90-91 -1.357651460516312 1102 92-93 -1.378848073636373 1102 94-95 -1.3878084780912552 1102 96-97 -1.3883479312229383 1102 98-99 -1.464403099879899 1102 100 -1.5125668390063076 1103 1 -0.4730115325554536 1103 2 -0.4543404556476034 1103 3 -0.48021165020694667 1103 4 -0.32863080080075235 1103 5 -0.3028385201042454 1103 6 -0.49330248848293223 1103 7 -0.47521517489893483 1103 8 -0.5346298539832972 1103 9 -0.6416108593947882 1103 10-11 -0.6259483225230227 1103 12-13 -0.7300382123204798 1103 14-15 -0.8124312379759928 1103 16-17 -0.8911503694344489 1103 18-19 -0.9306378083071607 1103 20-21 -0.8528597769883675 1103 22-23 -0.8841060592158314 1103 24-25 -1.0216992894224575 1103 26-27 -1.0429411609606518 1103 28-29 -1.0252222830108053 1103 30-31 -1.0340922151616496 1103 32-33 -1.0891892779015748 1103 34-35 -1.207336111711328 1103 36-37 -1.1368113656003231 1103 38-39 -1.1040266656606263 1103 40-41 -1.193525092047686 1103 42-43 -1.0188147368230602 1103 44-45 -1.0947200212862 1103 46-47 -1.1438257542315924 1103 48-49 -1.2332402552846276 1103 50-51 -1.2248198435955118 1103 52-53 -1.268694042070706 1103 54-55 -1.3309449427477134 1103 56-57 -1.1883683274697319 1103 58-59 -1.2648763212100107 1103 60-61 -1.4267359915989388 1103 62-63 -1.3317359782468756 1103 64-65 -1.3529665269597402 1103 66-67 -1.4341002477379803 1103 68-69 -1.3532778232581286 1103 70-71 -1.4385383361393629 1103 72-73 -1.4349973610601978 1103 74-75 -1.3506784386913928 1103 76-77 -1.1284946133158762 1103 78-79 -1.0373098200025588 1103 80-81 -0.9898508193230633 1103 82-83 -1.0943207190377713 1103 84-85 -1.2107180213035882 1103 86-87 -1.1560526141578649 1103 88-89 -1.2010377922181128 1103 90-91 -1.320080824800396 1103 92-93 -1.4552350952014734 1103 94-95 -1.3357124449284683 1103 96-97 -1.3521812722983881 1103 98-99 -1.4838816796667906 1103 100 -1.7164807201715178 1104 1 -0.5076247454490428 1104 2 -0.5174081800311434 1104 3 -0.5766188915844133 1104 4 -0.40762581259029673 1104 5 -0.4632892260520123 1104 6 -0.5174103443055316 1104 7 -0.5412556336574568 1104 8 -0.5271024994897573 1104 9 -0.8159216446488671 1104 10-11 -0.676280469733527 1104 12-13 -0.7045413646919201 1104 14-15 -0.9642770692992002 1104 16-17 -0.8851601857365452 1104 18-19 -0.9214433700089444 1104 20-21 -0.9924049385244089 1104 22-23 -0.8990443495649458 1104 24-25 -0.963487357928436 1104 26-27 -0.9123488560944395 1104 28-29 -1.080634423989281 1104 30-31 -1.065121560603906 1104 32-33 -0.938275983186756 1104 34-35 -0.9383346122600003 1104 36-37 -0.9719760902002861 1104 38-39 -1.0164992386213356 1104 40-41 -1.0006026759153102 1104 42-43 -0.9708708126064138 1104 44-45 -1.199114613277736 1104 46-47 -1.117321254927596 1104 48-49 -1.1720604257982075 1104 50-51 -1.0587067268250223 1104 52-53 -1.0870675372432288 1104 54-55 -1.1387289839288144 1104 56-57 -1.0521331300390955 1104 58-59 -1.152887680145426 1104 60-61 -1.1878151941001747 1104 62-63 -1.1724965702270111 1104 64-65 -1.1838869156114384 1104 66-67 -1.1674958235879203 1104 68-69 -1.2267018102648422 1104 70-71 -1.423273007008845 1104 72-73 -1.3238888552623251 1104 74-75 -1.3935185542740278 1104 76-77 -1.2005933969483813 1104 78-79 -0.9705443572669736 1104 80-81 -0.9390899829328738 1104 82-83 -0.9304805293548419 1104 84-85 -1.0603239036229972 1104 86-87 -1.0646851641181279 1104 88-89 -1.023511373910054 1104 90-91 -0.9977962290762115 1104 92-93 -1.1609949548151057 1104 94-95 -1.2744571333281094 1104 96-97 -1.2150369428965107 1104 98-99 -1.2898406557424167 1104 100 -1.4083150581769672 1105 1 -0.6000539054694904 1105 2 -0.4890778336476096 1105 3 -0.5795416886832712 1105 4 -0.3526130744298115 1105 5 -0.4237326700564523 1105 6 -0.621332589177932 1105 7 -0.664004387042155 1105 8 -0.6752028610030649 1105 9 -0.7811041770485474 1105 10-11 -0.7430488056021787 1105 12-13 -0.7796898397052132 1105 14-15 -1.0760698242523077 1105 16-17 -0.9787345997914301 1105 18-19 -0.9008457337024183 1105 20-21 -0.9679668648709523 1105 22-23 -1.0352903182091637 1105 24-25 -1.049476613120703 1105 26-27 -1.2014098966204756 1105 28-29 -1.0979066408397884 1105 30-31 -1.1367434332879895 1105 32-33 -1.0692737475934067 1105 34-35 -1.081106450352948 1105 36-37 -1.0456829256963331 1105 38-39 -1.1932584083519373 1105 40-41 -1.2458956638710248 1105 42-43 -1.0911750914376626 1105 44-45 -1.233088087931776 1105 46-47 -1.2177038103196054 1105 48-49 -1.1870788299253263 1105 50-51 -1.315982926275261 1105 52-53 -1.2822457175236082 1105 54-55 -1.1839299512489205 1105 56-57 -1.2459361330014147 1105 58-59 -1.3243870981521155 1105 60-61 -1.3111751944286354 1105 62-63 -1.2904977670598186 1105 64-65 -1.3025686443060707 1105 66-67 -1.4018677121818754 1105 68-69 -1.4669957630771933 1105 70-71 -1.391704488843878 1105 72-73 -1.25767710823542 1105 74-75 -1.337976171406595 1105 76-77 -1.3411570990417125 1105 78-79 -1.313657128478937 1105 80-81 -1.2449377326613806 1105 82-83 -1.1223091687632838 1105 84-85 -1.0503989674164451 1105 86-87 -1.07029929536737 1105 88-89 -1.139509980000149 1105 90-91 -1.3175068644650985 1105 92-93 -1.315684312035824 1105 94-95 -1.3595007776942758 1105 96-97 -1.4684362662651367 1105 98-99 -1.4345488789897125 1105 100 -1.2619750468039754 1106 1 -0.48623329908930657 1106 2 -0.5034022368475668 1106 3 -0.5679960650787343 1106 4 -0.3529725680545184 1106 5 -0.4650939619783756 1106 6 -0.4557278630306598 1106 7 -0.5472620244162272 1106 8 -0.5181338698983708 1106 9 -0.7229346098088953 1106 10-11 -0.6070607453339321 1106 12-13 -0.7389006616572118 1106 14-15 -0.8939453255411252 1106 16-17 -0.8863869389458046 1106 18-19 -0.8545123862745427 1106 20-21 -0.9045753749870968 1106 22-23 -0.8008033884057681 1106 24-25 -0.9104825610156837 1106 26-27 -0.994014886418519 1106 28-29 -0.9225456341249796 1106 30-31 -0.8867744412713066 1106 32-33 -0.9855172672150729 1106 34-35 -0.9497945017499276 1106 36-37 -0.8988167218279628 1106 38-39 -1.0708575447713145 1106 40-41 -1.0915375913073717 1106 42-43 -1.0717305157205672 1106 44-45 -1.0251326333041604 1106 46-47 -1.1010887959887796 1106 48-49 -1.056020044095149 1106 50-51 -1.1719427808859635 1106 52-53 -1.0443639524563366 1106 54-55 -1.1164358315730283 1106 56-57 -1.1081852605909717 1106 58-59 -1.145936299281999 1106 60-61 -1.2113020867239825 1106 62-63 -1.175559940553633 1106 64-65 -1.1386462670715645 1106 66-67 -1.1685793570061591 1106 68-69 -1.1327388436954564 1106 70-71 -1.1675335171249834 1106 72-73 -1.2445878840070748 1106 74-75 -1.2668966707512013 1106 76-77 -1.1414093290877823 1106 78-79 -1.070579142571816 1106 80-81 -1.0214981638215868 1106 82-83 -1.0488274821279724 1106 84-85 -0.9627117898808173 1106 86-87 -1.0602283956964378 1106 88-89 -1.0389770613778637 1106 90-91 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0.011041949936021922 2116 36-37 0.7695186663795184 2116 38-39 0.8277864545379288 2116 40-41 0.8349606312857034 2116 42-43 0.920301860363729 2116 44-45 0.8838933409023539 2116 46-47 0.8773174629797396 2116 48-49 0.9474879674426262 2116 50-51 0.8072194050635204 2116 52-53 0.8903375275164365 2116 54-55 1.0854396594265623 2116 56-57 0.2509167712475815 2116 58-59 0.3226187817972317 2116 60-61 0.6736296359504905 2116 62-63 0.7766421008925306 2116 64-65 0.9747728231469281 2116 66-67 1.0372972226493786 2116 68-69 0.7447518661211987 2116 70-71 0.8757773346185473 2116 72-73 0.817431894066516 2116 74-75 1.0467238702465522 2116 76-77 0.8918971173680426 2116 78-79 0.8056671661461792 2116 80-81 0.7298420352937107 2116 82-83 0.7515608998988235 2116 84-85 0.7388856057476936 2116 86-87 0.8197691576080075 2116 88-89 0.8673048449068972 2116 90-91 0.8640005612045734 2116 92-93 0.8583775826106681 2116 94-95 0.6156135085927836 2116 96-97 0.8117992039513773 2116 98-99 0.6506050470854774 2116 100 -0.00483776887056564 2201 1 0.7050461508616941 2201 2 0.6699703281257072 2201 3 0.6442926944584997 2201 4 0.43590997272602294 2201 5 0.563006179128017 2201 6 0.5718106274001826 2201 7 0.6003167943962637 2201 8 0.663595371333102 2201 9 0.7656260934403818 2201 10-11 0.7792536690398606 2201 12-13 0.8012484258421324 2201 14-15 0.9884473948688566 2201 16-17 -0.4989296668628569 2201 18-19 -0.0032804595950324256 2201 20-21 0.6678282583331736 2201 22-23 0.45239502587750735 2201 24-25 0.6854261629864595 2201 26-27 0.916024297321556 2201 28-29 0.9538662432573162 2201 30-31 1.0785186059644047 2201 32-33 1.12229351577097 2201 34-35 1.1381819883363775 2201 36-37 1.150002538277711 2201 38-39 1.1195336930553097 2201 40-41 1.2016857672427648 2201 42-43 1.2613678109720325 2201 44-45 1.2124022613334802 2201 46-47 1.2365711668442358 2201 48-49 1.1979458581392493 2201 50-51 1.2131637475846517 2201 52-53 1.2733821211175211 2201 54-55 1.3150664386327406 2201 56-57 1.3179533645871473 2201 58-59 1.3627300413352543 2201 60-61 1.2784446832147935 2201 62-63 1.1856507499691489 2201 64-65 -1.1340050180545447 2201 66-67 0.5710973882873063 2201 68-69 1.1683884878510398 2201 70-71 1.3105711083996638 2201 72-73 1.359845562188248 2201 74-75 1.3941124769665265 2201 76-77 1.4192776177204074 2201 78-79 1.1514254270751678 2201 80-81 1.0536278169487723 2201 82-83 1.1148066606965337 2201 84-85 1.0977252517773586 2201 86-87 1.1177318860670766 2201 88-89 1.1109536407333813 2201 90-91 1.1342953364605783 2201 92-93 1.1248379562490847 2201 94-95 1.150047635961613 2201 96-97 1.1689996168184784 2201 98-99 1.1954467919647094 2201 100 1.5033863163551118 2202 1 0.771094717187399 2202 2 0.6962533480964836 2202 3 0.7062668850738589 2202 4 0.5125939019353112 2202 5 0.5915545019076873 2202 6 0.5922486985156254 2202 7 0.575708799591915 2202 8 0.6397701789317409 2202 9 0.7643448205064658 2202 10-11 0.8234820827433893 2202 12-13 0.8292258897161418 2202 14-15 1.0786031993825773 2202 16-17 1.0533729796706766 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1.1956921165281322 2202 86-87 1.1331509735812801 2202 88-89 1.0764350240748328 2202 90-91 0.6373429480858377 2202 92-93 0.9960594532453477 2202 94-95 1.2386242100807898 2202 96-97 1.3252572300673826 2202 98-99 1.3410618463566593 2202 100 1.5923381072302512 2203 1 0.49496358936784546 2203 2 0.5557450272944777 2203 3 0.5780439778056916 2203 4 0.4024898417849343 2203 5 -0.7633573276662489 2203 6 0.08729411204946302 2203 7 0.381939476283641 2203 8 0.4876435122650733 2203 9 0.6764336926869134 2203 10-11 0.758166794522829 2203 12-13 0.8543444360820516 2203 14-15 1.0480565828412267 2203 16-17 1.0635653355603978 2203 18-19 1.012813912050106 2203 20-21 0.9708779933013716 2203 22-23 0.21110271204372566 2203 24-25 0.8930451098909913 2203 26-27 0.9830332941804869 2203 28-29 1.0268122468439742 2203 30-31 1.1779506981285692 2203 32-33 0.5130019676727571 2203 34-35 -0.20670429275846658 2203 36-37 0.2149407453394403 2203 38-39 0.8884181240548799 2203 40-41 1.0983983688881338 2203 42-43 0.5766541316812592 2203 44-45 1.1334793085545485 2203 46-47 1.2466554374330272 2203 48-49 1.1611959677044439 2203 50-51 1.144397854527604 2203 52-53 1.3012840880416547 2203 54-55 1.165465657271156 2203 56-57 1.0097860728800399 2203 58-59 1.2295973816905246 2203 60-61 1.2725822967932956 2203 62-63 1.2378557037198235 2203 64-65 1.2142354763255838 2203 66-67 1.3604891656483886 2203 68-69 1.308921528623948 2203 70-71 1.4489749144320356 2203 72-73 1.4116032518947321 2203 74-75 1.3848371271208961 2203 76-77 1.0711054108393476 2203 78-79 1.0751602695393387 2203 80-81 1.1757074009550585 2203 82-83 1.197661180398125 2203 84-85 1.2380596102624644 2203 86-87 1.2357167379923055 2203 88-89 1.3203579814683195 2203 90-91 1.3554779355545108 2203 92-93 1.3204355184082566 2203 94-95 1.3822925810081053 2203 96-97 1.3669933453556027 2203 98-99 1.3144921220458805 2203 100 1.6252318916914064 2204 1 0.8047897171874006 2204 2 0.731760848096485 2204 3 0.7274256350738568 2204 4 0.5327389019353106 2204 5 0.5974995019076843 2204 6 0.5953474485156249 2204 7 0.6561687995919172 2204 8 0.6333726789317353 2204 9 0.8164160705064631 2204 10-11 0.7985789577433806 2204 12-13 0.8718477647161365 2204 14-15 1.0686563243825802 2204 16-17 1.1017011046706742 2204 18-19 0.9822073174385721 2204 20-21 1.0609774607199256 2204 22-23 1.0765704830337057 2204 24-25 1.0724743470463807 2204 26-27 1.0843588737544252 2204 28-29 1.1435180739116433 2204 30-31 1.2688617648578884 2204 32-33 1.2859460466201256 2204 34-35 1.276888086627494 2204 36-37 1.3161878083093725 2204 38-39 1.3516760736162752 2204 40-41 1.2617724290385084 2204 42-43 -0.34316005221598544 2204 44-45 0.9915498755971583 2204 46-47 1.3524611626836531 2204 48-49 1.434074452980127 2204 50-51 1.4587562113571764 2204 52-53 1.2832744154226035 2204 54-55 1.3795863104666495 2204 56-57 1.4777009773286593 2204 58-59 1.5069706977932782 2204 60-61 1.46380194748253 2204 62-63 1.5069747904992639 2204 64-65 1.5110094424909946 2204 66-67 1.5241032790569093 2204 68-69 1.5448938813808368 2204 70-71 1.6226612787804058 2204 72-73 1.5878281892380954 2204 74-75 1.5348982173464591 2204 76-77 1.457433340037344 2204 78-79 1.2920599405919688 2204 80-81 1.300878727709442 2204 82-83 1.2240509830297093 2204 84-85 1.3376558665281344 2204 86-87 1.3653640985812814 2204 88-89 1.3740500240748332 2204 90-91 1.477326698085836 2204 92-93 1.4582125782453481 2204 94-95 1.5052098350807874 2204 96-97 1.5323753550673835 2204 98-99 1.4633505963566584 2204 100 1.5609681072302521 2205 1 0.767499718727283 2205 2 0.7572496646964169 2205 3 0.7729227766660998 2205 4 0.5031148258650902 2205 5 0.6017605505679882 2205 6 0.6618586030428801 2205 7 0.6386903579534788 2205 8 0.6793140286391619 2205 9 0.8928988334414854 2205 10-11 0.8163855628788923 2205 12-13 0.9098124003413304 2205 14-15 1.126140372157444 2205 16-17 1.1716803403112621 2205 18-19 1.0833978875802472 2205 20-21 1.122065869250875 2205 22-23 1.1501263374762658 2205 24-25 1.09718723105469 2205 26-27 1.1524994169481388 2205 28-29 1.1734947831817308 2205 30-31 1.2174139620783961 2205 32-33 1.2891379352865826 2205 34-35 1.3565082408082745 2205 36-37 1.4006403558532554 2205 38-39 1.3691945762725766 2205 40-41 1.3723564296544666 2205 42-43 1.46623311359091 2205 44-45 1.494501783127184 2205 46-47 1.5488981862823579 2205 48-49 1.539308972344159 2205 50-51 1.5771558157997347 2205 52-53 1.533442527333598 2205 54-55 1.5045184353126615 2205 56-57 1.5449636669267548 2205 58-59 1.5078620734862298 2205 60-61 1.6098805151988813 2205 62-63 1.6072143144829454 2205 64-65 1.1182610401195774 2205 66-67 1.4437711936704076 2205 68-69 1.4837322802875192 2205 70-71 1.6472945219664226 2205 72-73 1.6015993626289173 2205 74-75 1.5797767590772906 2205 76-77 1.4664305393751924 2205 78-79 1.333546553234413 2205 80-81 1.3753687617793524 2205 82-83 1.3801702373413782 2205 84-85 1.4008133484345073 2205 86-87 1.3953519960635745 2205 88-89 1.4287002781555174 2205 90-91 1.3968385070633573 2205 92-93 1.4618261301779008 2205 94-95 1.6175361410940319 2205 96-97 1.6300336021031079 2205 98-99 1.6423174792485575 2205 100 1.9450684306056765 2206 1 0.6135443217581376 2206 2 0.6213257161614365 2206 3 0.6417691313526106 2206 4 0.4696751709174194 2206 5 -0.25795259176243945 2206 6 0.32782693938165863 2206 7 0.48309971223662984 2206 8 0.5849878965681867 2206 9 0.7923362563807714 2206 10-11 0.7779176262706713 2206 12-13 0.8591950973540889 2206 14-15 1.1095166860763115 2206 16-17 1.0819028248067397 2206 18-19 1.080867128933086 2206 20-21 1.058378650390651 2206 22-23 1.0916547631231666 2206 24-25 1.0970856719411302 2206 26-27 1.1367005094107157 2206 28-29 1.159037826580409 2206 30-31 1.2046039347383584 2206 32-33 1.2752777730522453 2206 34-35 1.2796902145969753 2206 36-37 1.377712144405443 2206 38-39 1.3399637958572939 2206 40-41 1.327824338525808 2206 42-43 1.4155084633267947 2206 44-45 1.374288819928836 2206 46-47 1.3763476200772757 2206 48-49 1.4019046547355032 2206 50-51 1.463730099212384 2206 52-53 1.4674204907308308 2206 54-55 1.5083217332414165 2206 56-57 1.50452829887805 2206 58-59 1.543502457585042 2206 60-61 1.602928664799471 2206 62-63 1.5855261244152175 2206 64-65 1.6400629212911753 2206 66-67 1.635337844347852 2206 68-69 1.6513047829369967 2206 70-71 1.4051352606064498 2206 72-73 1.5280018473346217 2206 74-75 1.5784395081297973 2206 76-77 1.521692403524039 2206 78-79 1.3923039123631327 2206 80-81 1.3662733229770758 2206 82-83 1.3833230614765561 2206 84-85 1.3925625299997897 2206 86-87 1.4765537340684318 2206 88-89 1.5009507720826463 2206 90-91 1.50914999063583 2206 92-93 1.4719676174311864 2206 94-95 1.5560775734847887 2206 96-97 1.6134955461100837 2206 98-99 1.58315812060113 2206 100 1.8483096724084227 2207 1 0.7708554466706836 2207 2 0.7098317067590969 2207 3 0.6921810492075977 2207 4 0.4698950569505058 2207 5 0.567254441117413 2207 6 0.5822229234396374 2207 7 0.6653861248198751 2207 8 0.659971273156664 2207 9 0.7876219025733349 2207 10-11 0.799266885174994 2207 12-13 0.8830885036979055 2207 14-15 1.0451150534859224 2207 16-17 1.0856161407648983 2207 18-19 1.0437133014811266 2207 20-21 1.0593921643673383 2207 22-23 1.0948106521066592 2207 24-25 1.1535507623199308 2207 26-27 1.1730865523258487 2207 28-29 1.1089910982277544 2207 30-31 1.1325179673654873 2207 32-33 1.2346495040669296 2207 34-35 1.285433631840256 2207 36-37 1.307971860361043 2207 38-39 1.3351959729323823 2207 40-41 1.2954768363333429 2207 42-43 1.3377586882855326 2207 44-45 1.3658474629831758 2207 46-47 1.4199903705103978 2207 48-49 1.4256409898342355 2207 50-51 1.3648492489711614 2207 52-53 1.3505115449818703 2207 54-55 1.4189700965608694 2207 56-57 1.4560100103833733 2207 58-59 1.391651786319116 2207 60-61 1.4250322381360192 2207 62-63 1.5125800811527625 2207 64-65 1.523309024557868 2207 66-67 1.4770881764733232 2207 68-69 1.5516050325966404 2207 70-71 1.566472781439984 2207 72-73 1.5456701345268442 2207 74-75 1.4943036124832432 2207 76-77 1.3646441113139396 2207 78-79 1.2855226115646161 2207 80-81 1.2632920540772226 2207 82-83 1.2024234184400378 2207 84-85 1.332888104370081 2207 86-87 1.2873091498730815 2207 88-89 1.3875625620687515 2207 90-91 1.3558492094840169 2207 92-93 1.4047396964064411 2207 94-95 1.4869411705671105 2207 96-97 1.5262182027117603 2207 98-99 1.5390748554751994 2207 100 1.7775828488715888 2208 1 0.8369138141169579 2208 2 0.7757774420158086 2208 3 0.7552252475061252 2208 4 0.45093991036397085 2208 5 0.5726391015464571 2208 6 0.6557463986962375 2208 7 0.6989698230717423 2208 8 0.668947445638544 2208 9 0.9264588722825025 2208 10-11 0.8681893675115973 2208 12-13 0.9009611094632817 2208 14-15 1.1451363721700574 2208 16-17 1.1619051891830168 2208 18-19 1.1182909078961316 2208 20-21 1.178203341213603 2208 22-23 1.2231847684792214 2208 24-25 1.2088210978741927 2208 26-27 1.278861902803186 2208 28-29 1.271229166626874 2208 30-31 1.3340879557820386 2208 32-33 1.3793188265719678 2208 34-35 1.396807515811716 2208 36-37 1.3588404366206248 2208 38-39 1.4159168829931588 2208 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42-43 1.480789983147325 2310 44-45 1.4196647319104372 2310 46-47 1.1112512255931222 2310 48-49 1.381697433548922 2310 50-51 1.5053887400200736 2310 52-53 1.5524664102856178 2310 54-55 1.5920175846625995 2310 56-57 1.6062682513757167 2310 58-59 1.593270159898701 2310 60-61 1.5866784271579277 2310 62-63 1.0101553946535304 2310 64-65 1.1971803403468684 2310 66-67 1.5437201454952643 2310 68-69 1.6714145968364633 2310 70-71 1.6908486764129265 2310 72-73 1.7300289039492327 2310 74-75 1.6815334913190654 2310 76-77 1.6676619917645645 2310 78-79 1.4761469157259768 2310 80-81 1.4201054344545305 2310 82-83 1.4708432514930756 2310 84-85 1.5002332005066954 2310 86-87 1.4754493893049272 2310 88-89 1.521527317106397 2310 90-91 1.6091282726838152 2310 92-93 1.7086567135193214 2310 94-95 1.6618542755959744 2310 96-97 1.6469934778339095 2310 98-99 1.713338963688404 2310 100 1.7812800488621754 2311 1 0.4815853696822323 2311 2 0.45164745497572767 2311 3 0.5010272275598311 2311 4 0.3913226412326267 2311 5 0.44398551460328406 2311 6 0.5329237974232157 2311 7 0.57990588255619 2311 8 0.5705893345266588 2311 9 0.7478465860807191 2311 10-11 0.7348400986866963 2311 12-13 0.8228020337964495 2311 14-15 0.9484104624472351 2311 16-17 0.8195152884985433 2311 18-19 0.9842021229449287 2311 20-21 0.9263259438908733 2311 22-23 1.0671936146767536 2311 24-25 1.1010497594688786 2311 26-27 0.48565034666555107 2311 28-29 0.8159485064478034 2311 30-31 1.0401538144223963 2311 32-33 1.1845717662835469 2311 34-35 1.207984027910335 2311 36-37 1.2352174390666804 2311 38-39 1.2416514620499939 2311 40-41 1.2852862318138278 2311 42-43 1.2393700029582106 2311 44-45 1.2762138020069642 2311 46-47 1.0028195412266072 2311 48-49 1.2477926909119361 2311 50-51 1.3735410735139553 2311 52-53 1.2970478275498536 2311 54-55 1.2748282050267363 2311 56-57 1.3996093078940604 2311 58-59 1.4274660430471897 2311 60-61 1.491731323589697 2311 62-63 1.4947131790068013 2311 64-65 1.5301014671440853 2311 66-67 1.6034570120802343 2311 68-69 1.555064801442839 2311 70-71 1.5523127264110528 2311 72-73 1.6162500965306847 2311 74-75 1.5792245016688682 2311 76-77 1.4450675388120722 2311 78-79 1.1969824845098884 2311 80-81 1.1465576332674594 2311 82-83 1.2439590414147084 2311 84-85 1.3375508233483266 2311 86-87 1.360890306324123 2311 88-89 1.397724920738547 2311 90-91 1.4295341733589417 2311 92-93 1.5146665982113952 2311 94-95 1.5211550161038154 2311 96-97 1.537493569010401 2311 98-99 1.5218359355211106 2311 100 1.5539261084112397 2312 1 0.39981558864709044 2312 2 0.5268246189752048 2312 3 0.5908557594384192 2312 4 0.3138422969970378 2312 5 0.47814901171160784 2312 6 0.5599449657632647 2312 7 0.45462377417433686 2312 8 0.575016424392885 2312 9 0.7615053724309391 2312 10-11 0.7842748205792631 2312 12-13 0.851477451081422 2312 14-15 1.1224893286491024 2312 16-17 1.045242260716428 2312 18-19 1.0104946948895588 2312 20-21 1.0461996375536202 2312 22-23 1.149904100045326 2312 24-25 1.123050611116824 2312 26-27 1.1880699530935601 2312 28-29 1.2087436548847705 2312 30-31 1.218707273663263 2312 32-33 1.0893308959302246 2312 34-35 1.196573714350805 2312 36-37 1.2373815832730557 2312 38-39 1.0285215143388626 2312 40-41 1.1764084130617434 2312 42-43 1.281064087671453 2312 44-45 1.0119330682260639 2312 46-47 1.089420301663317 2312 48-49 1.2570299835719965 2312 50-51 1.0913852849773704 2312 52-53 1.2985906032403278 2312 54-55 1.2171038417847342 2312 56-57 1.3005526816678028 2312 58-59 1.408798550462123 2312 60-61 1.4075921730634988 2312 62-63 1.4525292680773347 2312 64-65 1.486244781997165 2312 66-67 1.4644451681273551 2312 68-69 1.4557647737192525 2312 70-71 1.3412085622771386 2312 72-73 1.4082820744959008 2312 74-75 1.4334380684720927 2312 76-77 1.3936305758761272 2312 78-79 1.2300994966921905 2312 80-81 1.2277302958830028 2312 82-83 1.239579872826372 2312 84-85 1.3004228645310398 2312 86-87 1.3715718305166504 2312 88-89 1.3612545447719953 2312 90-91 1.3671507040771225 2312 92-93 1.4058781896287904 2312 94-95 1.4499521984613253 2312 96-97 1.1348752983317247 2312 98-99 0.9154980409826585 2312 100 1.185649479779272 2313 1 0.0902430590427663 2313 2 0.3357493890022525 2313 3 0.3764519544675764 2313 4 0.28381587417782583 2313 5 0.41188911111148485 2313 6 0.41817033474644916 2313 7 0.4717513415933752 2313 8 0.5622082797352448 2313 9 0.7531320812807536 2313 10-11 0.47429669194718116 2313 12-13 0.5783703747417022 2313 14-15 0.6810914389735174 2313 16-17 0.9500040972747641 2313 18-19 0.8618259943925537 2313 20-21 0.8525066334737517 2313 22-23 0.8517766668357467 2313 24-25 0.7841291247125568 2313 26-27 0.9808737797076716 2313 28-29 0.7915024603250842 2313 30-31 1.0622869954093872 2313 32-33 1.0951304421643187 2313 34-35 1.1143097333586809 2313 36-37 1.1904414263699934 2313 38-39 1.2279703006980895 2313 40-41 1.2291674253862084 2313 42-43 1.2559659635802163 2313 44-45 1.090604956860851 2313 46-47 1.1624033536076794 2313 48-49 1.265361261325637 2313 50-51 1.2752268902286161 2313 52-53 1.2903067496994538 2313 54-55 1.2445888784907524 2313 56-57 0.9960592109846189 2313 58-59 1.0899081178991992 2313 60-61 0.9273709873839167 2313 62-63 1.2661423512187682 2313 64-65 1.392708903776608 2313 66-67 1.3178538497289338 2313 68-69 1.4871111704604587 2313 70-71 1.440983217695674 2313 72-73 1.4417398035551088 2313 74-75 1.566011712598467 2313 76-77 1.4067648548291594 2313 78-79 1.2413197789776547 2313 80-81 1.1828931428847547 2313 82-83 1.2545090043956613 2313 84-85 1.237487061743618 2313 86-87 1.299084823106483 2313 88-89 1.3164135193268436 2313 90-91 1.116586308202713 2313 92-93 1.3467086862621507 2313 94-95 1.487977080789335 2313 96-97 1.5392000788621232 2313 98-99 1.5318318098336512 2313 100 1.6273057624530445 2314 1 -0.27457804587527335 2314 2 0.08956104530302156 2314 3 0.36068589795560513 2314 4 0.3079830581084195 2314 5 0.45758589284704243 2314 6 0.46434045130184387 2314 7 0.5870390413056228 2314 8 0.5922986722013874 2314 9 0.5789592885915695 2314 10-11 0.7080091054673616 2314 12-13 0.6067749995728491 2314 14-15 0.8800115652821248 2314 16-17 0.9395168499847557 2314 18-19 0.9881300043324828 2314 20-21 1.084612890955846 2314 22-23 1.1521778543066858 2314 24-25 0.9655387490039686 2314 26-27 0.8857909958053654 2314 28-29 0.9625444344558574 2314 30-31 1.0284508085870812 2314 32-33 1.0178561909970298 2314 34-35 1.147081482469872 2314 36-37 1.1270448086893126 2314 38-39 0.8686812819496126 2314 40-41 0.8128997629507992 2314 42-43 1.0453393801367454 2314 44-45 1.1473209034231857 2314 46-47 1.1240502923514768 2314 48-49 1.1514551452836486 2314 50-51 1.0687861428776557 2314 52-53 1.1602090410015578 2314 54-55 1.0962207840699136 2314 56-57 1.1122844350468526 2314 58-59 1.198924908333879 2314 60-61 1.3563500844234753 2314 62-63 1.2488660849159743 2314 64-65 1.262225655039849 2314 66-67 0.9591550457742386 2314 68-69 0.9742276986843521 2314 70-71 1.1094420968257097 2314 72-73 1.2136937269425374 2314 74-75 1.3429647790775334 2314 76-77 1.1151181307089324 2314 78-79 1.0182046205384125 2314 80-81 1.0333673182254408 2314 82-83 1.048320074974999 2314 84-85 1.1500215534944083 2314 86-87 1.1720645413913857 2314 88-89 1.1302460184300216 2314 90-91 1.2708948021891828 2314 92-93 1.1943583098092532 2314 94-95 1.1955929291428795 2314 96-97 1.1894039025236012 2314 98-99 1.3236782713096211 2314 100 1.3995903752864116 2315 1 0.4540138272778229 2315 2 0.48201557574109444 2315 3 0.5024271376933527 2315 4 0.40167612383856977 2315 5 0.4090985581729356 2315 6 0.5341830978266628 2315 7 0.4857005205622045 2315 8 0.4043729749702081 2315 9 0.6593015709011851 2315 10-11 0.7078345443480885 2315 12-13 0.5876309995194973 2315 14-15 0.7707425994112853 2315 16-17 0.9532702094682932 2315 18-19 0.941201078203612 2315 20-21 0.7189639057464774 2315 22-23 1.0564526395393727 2315 24-25 1.0462034785771692 2315 26-27 1.0282419789288184 2315 28-29 0.9891416385736207 2315 30-31 1.0404890232725563 2315 32-33 1.0117680019093527 2315 34-35 1.0309104083764495 2315 36-37 1.0982257079612978 2315 38-39 1.1523279974177711 2315 40-41 1.1127723213881566 2315 42-43 1.2302650567799915 2315 44-45 1.2755768734082693 2315 46-47 1.2267116134694973 2315 48-49 1.1855133232794017 2315 50-51 1.2132079683006225 2315 52-53 1.3297289847314886 2315 54-55 1.10355756558004 2315 56-57 1.1375813172346412 2315 58-59 1.1183615291231206 2315 60-61 1.2166468210830672 2315 62-63 1.312817457267606 2315 64-65 1.394985104540659 2315 66-67 1.4173956529265794 2315 68-69 1.4121899961002313 2315 70-71 1.3062669936684514 2315 72-73 1.4023272562683786 2315 74-75 0.8261850875919023 2315 76-77 0.7662151193182432 2315 78-79 1.0467679614402527 2315 80-81 1.0565362583000812 2315 82-83 0.973193435842969 2315 84-85 1.099110379769126 2315 86-87 1.09557151613906 2315 88-89 1.1537937265292584 2315 90-91 1.1809776373351575 2315 92-93 1.3149611900043574 2315 94-95 1.3597161348690783 2315 96-97 1.4208217025806604 2315 98-99 1.3983673494426334 2315 100 1.2062564307554418 2316 1 0.3731306343893941 2316 2 0.16661474709719215 2316 3 0.3238724810767124 2316 4 0.1755971282022628 2316 5 0.3960144019790661 2316 6 0.469058748479938 2316 7 0.5376052735390928 2316 8 0.5860107499524361 2316 9 0.7619258626192433 2316 10-11 0.7567089207162567 2316 12-13 0.5268170388952953 2316 14-15 0.6846029138722329 2316 16-17 0.9256442145029382 2316 18-19 0.9036298504507059 2316 20-21 0.892593047800581 2316 22-23 0.9121520430800985 2316 24-25 0.574050286107763 2316 26-27 0.8942474578015265 2316 28-29 1.062069554996576 2316 30-31 0.749698053669448 2316 32-33 0.9382825473339054 2316 34-35 0.9799842567738182 2316 36-37 1.0657065114678304 2316 38-39 0.9588311748832297 2316 40-41 0.9482990171898322 2316 42-43 0.9912553764956513 2316 44-45 0.8614652262395524 2316 46-47 0.9935306554566736 2316 48-49 1.0973465019630027 2316 50-51 1.1070616070566714 2316 52-53 1.1417182167252449 2316 54-55 1.1258968141247507 2316 56-57 1.252768730272983 2316 58-59 1.18865794128007 2316 60-61 1.238833007885809 2316 62-63 1.2404600800246115 2316 64-65 1.3204716569806507 2316 66-67 1.256701088657202 2316 68-69 1.287570875492186 2316 70-71 1.2361589478560688 2316 72-73 1.2632350414864888 2316 74-75 1.3257435956476726 2316 76-77 1.110445362699732 2316 78-79 0.8538812958739044 2316 80-81 0.8915748264781733 2316 82-83 0.775472846876955 2316 84-85 0.8531547289478318 2316 86-87 0.9184698600909194 2316 88-89 0.9186150669013848 2316 90-91 0.7398616061871941 2316 92-93 0.5476406095622686 2316 94-95 0.5706744028002753 2316 96-97 0.8404699638289799 2316 98-99 1.1620485130233256 2316 100 0.6835207482009871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 8.0 12 33.0 13 206.0 14 637.0 15 1894.0 16 4518.0 17 9039.0 18 14289.0 19 20245.0 20 27532.0 21 37326.0 22 44833.0 23 47212.0 24 49574.0 25 56622.0 26 67516.0 27 83600.0 28 103785.0 29 127167.0 30 157166.0 31 195553.0 32 246327.0 33 318233.0 34 435712.0 35 587691.0 36 854747.0 37 1144811.0 38 830018.0 39 94891.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.902998606905605 20.24818811379624 13.629172807027642 24.219640472270516 2 15.658878765156379 20.419406637787958 40.10826819439724 23.81344640265842 3 18.841003454296654 25.44804259519995 29.949543173313625 25.761410777189774 4 12.608516086697028 16.50536379128792 37.93397890459146 32.95214121742359 5 13.148660628390685 37.54644239811983 34.68701842591244 14.617878547577048 6 31.239410989374576 36.99397071490095 17.728094166895932 14.038524128828541 7 27.674181912341716 31.980637063623647 22.170975735190986 18.174205288843652 8 26.23985154256553 35.925152710121395 19.702473751121612 18.13252199619146 9 26.410401661676243 15.61204175770818 20.63494375657522 37.34261282404036 10-11 25.673422234961357 26.06997579913779 27.651164130972184 20.605437834928665 12-13 25.60137330368979 23.586507055686663 28.2045580908252 22.60756154979835 14-15 22.76839992782112 25.15448375332337 25.894622970092605 26.182493348762904 16-17 22.16941567881418 27.8316047881569 26.86456173550953 23.134417797519394 18-19 21.830975025129515 27.20093181495327 29.0593290284993 21.908764131417914 20-21 22.793050442364972 26.56798991798246 28.72765416960781 21.911305470044756 22-23 22.679534416195096 26.40622780376142 28.57503519930087 22.33920258074261 24-25 22.32813461610216 26.32796558623917 29.042666104432584 22.301233693226084 26-27 22.351437892712653 26.600354350594902 28.51948667534951 22.528721081342937 28-29 22.02659632669784 26.982566564317313 28.7348111188243 22.256025990160545 30-31 22.66727026861785 26.623062869309088 28.712646509838052 21.997020352235005 32-33 22.660208688039273 26.767579851437596 28.292949535434502 22.279261925088623 34-35 22.68701883666898 26.754859803040326 28.643994555527723 21.91412680476297 36-37 22.616444076346028 26.639006522794705 28.396348702721387 22.348200698137884 38-39 22.56816861710781 26.770610657607403 28.616228309392756 22.044992415892033 40-41 22.521875332386102 26.237173099533717 28.91764251254986 22.323309055530316 42-43 22.43740024043443 26.500833860274525 28.45664056940398 22.605125329887066 44-45 22.324696769534054 26.963584289671193 28.425704894164717 22.286014046630033 46-47 22.385063094081076 26.710951702328018 27.998470577317498 22.90551462627341 48-49 22.702286389288044 26.713838906722287 28.049785300912294 22.534089403077378 50-51 22.563044485730682 26.796795043445563 27.90956323888792 22.730597231935835 52-53 22.37097340237423 27.124819861939187 27.6872615337139 22.816945201972676 54-55 22.30848496401506 26.980538281427023 27.804388328004233 22.906588426553682 56-57 22.783911034476496 26.798905732979893 27.52436517129368 22.89281806124993 58-59 22.26411918430883 27.042392979917036 27.948002169498547 22.74548566627559 60-61 22.512425531777154 27.31440196413225 27.59601857891011 22.577153925180486 62-63 22.614298988829155 27.03969653635886 27.42861047512398 22.917393999688013 64-65 22.199526762030768 26.990231518576913 27.558867168061358 23.251374551330958 66-67 22.31045558955828 26.839453254387198 27.566137842204043 23.283953313850482 68-69 22.10914082768696 26.89147808439514 27.702135966348457 23.297245121569446 70-71 22.15702799226089 26.647604033340897 27.76703000988483 23.428337964513386 72-73 22.36939827796729 26.85542615699775 27.652238440141485 23.12293712489347 74-75 22.525531181413154 27.021262186384163 27.377896920842705 23.075309711359974 76-77 22.388535152143174 26.860358594867588 27.31901222638664 23.43209402660259 78-79 22.23541565936198 26.916703931507385 27.454918235109567 23.39296217402107 80-81 22.05762349909209 27.203120802339953 27.475646154183664 23.26360954438429 82-83 22.50090210399793 26.763761717908213 27.925164402382634 22.810171775711225 84-85 22.659038289436793 27.008792860935603 27.290629280853878 23.041539568773725 86-87 22.02200047371464 27.24746156510963 27.57297171016832 23.15756625100741 88-89 22.297944635789733 26.71724264133334 27.523654857298936 23.46115786557799 90-91 22.404523171973562 26.70801112245376 27.533263154618677 23.354202550954 92-93 22.493627622927967 26.791703767662323 27.46095176420856 23.253716845201154 94-95 21.86998726469059 26.77370632932199 27.75659922009156 23.599707185895863 96-97 21.985513026803112 26.7451999605826 27.592871852452333 23.676415160161955 98-99 21.700769741631383 26.983780974130767 27.38777505145025 23.927674232787602 100 21.92956610988257 26.735547878504 27.31397090275147 24.02091510886196 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1528.0 1 1102.5 2 1464.5 3 2052.5 4 1360.0 5 932.5 6 1277.5 7 2384.0 8 3688.5 9 4091.0 10 3548.5 11 2959.5 12 3049.0 13 3500.5 14 4031.0 15 4757.5 16 5436.0 17 6081.5 18 6855.5 19 7529.0 20 8375.5 21 9424.5 22 10859.5 23 12976.5 24 15370.0 25 18611.5 26 23404.5 27 28730.0 28 35147.0 29 44234.5 30 55476.5 31 66485.0 32 79926.0 33 94598.0 34 106893.0 35 119543.5 36 132713.0 37 149371.0 38 160211.0 39 161552.5 40 160736.5 41 160139.0 42 164280.0 43 172467.5 44 183600.5 45 201193.0 46 217483.5 47 228355.5 48 236321.0 49 237423.5 50 233444.0 51 232511.0 52 228129.5 53 216937.5 54 205513.5 55 185666.5 56 164654.0 57 142816.0 58 121779.0 59 107588.0 60 87483.5 61 66539.5 62 52165.5 63 41193.5 64 30551.0 65 20544.5 66 14234.5 67 10651.0 68 8322.0 69 6482.0 70 5179.5 71 3946.0 72 3098.5 73 2657.0 74 2060.5 75 1493.5 76 1027.0 77 635.0 78 403.5 79 266.0 80 166.0 81 108.0 82 65.0 83 29.5 84 23.5 85 17.0 86 10.5 87 8.5 88 6.0 89 4.0 90 3.5 91 2.0 92 1.0 93 1.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042113288702597826 2 0.0 3 0.0 4 0.03373378719302883 5 0.0 6 0.0 7 8.990881448035403E-4 8 0.0 9 0.009710151963878234 10-11 4.4055319095373475E-4 12-13 3.596352579214161E-5 14-15 1.1688145882446023E-4 16-17 1.8880851040874345E-4 18-19 0.0 20-21 2.6972644344106207E-5 22-23 0.0 24-25 7.642249230830092E-4 26-27 5.574346497781949E-4 28-29 1.5284498461660184E-4 30-31 0.0023376291764892047 32-33 0.01497880849242698 34-35 0.02557905771966072 36-37 0.030326243124223416 38-39 0.02173096045990157 40-41 0.001339641335757275 42-43 1.1688145882446023E-4 44-45 0.010213641324968216 46-47 0.016579185390177283 48-49 0.0027152461973066915 50-51 0.009943914881527155 52-53 0.009431434638989137 54-55 0.009530334334917527 56-57 4.4055319095373475E-4 58-59 0.00823564740640043 60-61 0.004693240115874481 62-63 0.0011238601810044254 64-65 0.03291561698125761 66-67 0.05644475373076626 68-69 0.05484437683301595 70-71 0.04803827957685315 72-73 0.04230209721300657 74-75 0.03345506986813973 76-77 0.029795781118789322 78-79 0.0137650394969422 80-81 0.001006978722179965 82-83 0.03531618232788306 84-85 0.047588735504451384 86-87 0.053792443703595816 88-89 0.06492315493626365 90-91 0.04539496043113075 92-93 0.03668279630798444 94-95 0.01196686320733512 96-97 0.003488462001837736 98-99 0.021712978697005496 100 0.0317198297486689 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5561190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.96368316832661 #Duplication Level Percentage of deduplicated Percentage of total 1 80.10243446750201 56.04261346095956 2 13.020353558353879 18.219037821925298 3 3.6327960551888543 7.624913766611392 4 1.3178555994451717 3.6880812648474857 5 0.6430381000712088 2.2494656949272374 6 0.35483649612333773 1.4895400914799415 7 0.2144839543998726 1.050426120122575 8 0.14849839192507025 0.8311595554921284 9 0.10387317489284524 0.6540614908102084 >10 0.435422742339886 5.044795846548273 >50 0.01772146726165504 0.8388714567899582 >100 0.007853521013184993 0.9571349425238188 >500 5.35160904009216E-4 0.25256574518755104 >1k 2.0811740538642965E-4 0.30404738617358484 >5k 2.9731057911985033E-5 0.18067699228891446 >10k+ 5.9462115823970065E-5 0.5726083633121387 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14830 0.26666954374873003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12698 0.22833242525430708 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8686 0.156189592515271 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.596352579214161E-5 0.0 0.0 0.0 6 0.0 3.596352579214161E-5 0.0 0.0 0.0 7 0.0 3.596352579214161E-5 0.0 0.0 0.0 8 0.0 3.596352579214161E-5 0.0 0.0 0.0 9 0.0 3.596352579214161E-5 0.0 0.0 0.0 10-11 0.0 3.596352579214161E-5 0.0 8.990881448035403E-6 0.0 12-13 0.0 5.3945288688212413E-5 0.0 1.7981762896070806E-5 5.3945288688212413E-5 14-15 0.0 7.192705158428322E-5 0.0 1.7981762896070806E-5 5.3945288688212413E-5 16-17 0.0 7.192705158428322E-5 0.0 1.7981762896070806E-5 5.3945288688212413E-5 18-19 0.0 7.192705158428322E-5 0.0 3.596352579214161E-5 5.3945288688212413E-5 20-21 0.0 7.192705158428322E-5 0.0 6.293617013624782E-5 6.293617013624782E-5 22-23 0.0 7.192705158428322E-5 0.0 2.337629176489205E-4 7.192705158428322E-5 24-25 0.0 7.192705158428322E-5 0.0 7.642249230830093E-4 8.091793303231863E-5 26-27 0.0 7.192705158428322E-5 0.0 0.001456522794581735 8.990881448035403E-5 28-29 0.0 7.192705158428322E-5 0.0 0.0035783708163180904 8.990881448035403E-5 30-31 0.0 7.192705158428322E-5 0.0 0.013405404239020785 8.990881448035403E-5 32-33 0.0 7.192705158428322E-5 0.0 0.03691655922563336 8.990881448035403E-5 34-35 0.0 7.192705158428322E-5 0.0 0.0716932886666343 8.990881448035403E-5 36-37 0.0 8.990881448035402E-5 0.0 0.12732886306707736 8.990881448035403E-5 38-39 0.0 1.0789057737642483E-4 0.0 0.21083616995643017 8.990881448035403E-5 40-41 0.0 1.0789057737642483E-4 0.0 0.305267397805146 8.990881448035403E-5 42-43 0.0 1.0789057737642483E-4 0.0 0.3933600542329969 1.0789057737642483E-4 44-45 0.0 1.2587234027249564E-4 0.0 0.49609885653969743 1.2587234027249564E-4 46-47 0.0 1.6183586606463725E-4 0.0 0.6197324673316322 1.2587234027249564E-4 48-49 0.0 1.8880851040874345E-4 0.0 0.7557375309960638 1.2587234027249564E-4 50-51 0.0 2.1578115475284965E-4 0.0 0.8945297679093863 1.2587234027249564E-4 52-53 0.0 2.1578115475284965E-4 0.0 1.0293030808154369 1.5284498461660186E-4 54-55 0.0 2.7871732488909744E-4 0.0 1.1851600107171307 1.7082674751267264E-4 56-57 0.0 2.877082063371329E-4 0.0 1.36181284940813 1.7981762896070806E-4 58-59 0.0 3.416534950253453E-4 0.0 1.5596392139092532 1.7981762896070806E-4 60-61 0.0 3.416534950253453E-4 0.0 1.7592367820556392 1.8880851040874345E-4 62-63 0.0 3.416534950253453E-4 0.0 1.964921176942345 2.3376291764892046E-4 64-65 0.0 3.955987837135577E-4 0.0 2.1768542344354356 2.4275379909695588E-4 66-67 0.0 4.135805466096285E-4 0.0 2.413422307096143 2.5174468054499127E-4 68-69 0.0 4.135805466096285E-4 0.0 2.6758211821570566 2.5174468054499127E-4 70-71 0.0 4.135805466096285E-4 0.0 2.9514726164723735 2.6972644344106205E-4 72-73 0.0 4.135805466096285E-4 0.0 3.2274567134012684 2.877082063371329E-4 74-75 0.0 4.135805466096285E-4 0.0 3.5025057586595674 2.877082063371329E-4 76-77 0.0 4.135805466096285E-4 0.0 3.812745113905477 2.877082063371329E-4 78-79 0.0 4.315623095056993E-4 0.0 4.146981131736194 2.877082063371329E-4 80-81 0.0 4.315623095056993E-4 0.0 4.496321830399609 2.877082063371329E-4 82-83 0.0 4.315623095056993E-4 0.0 4.856370668867635 2.966990877851683E-4 84-85 0.0 4.315623095056993E-4 0.0 5.2142257322623395 3.506443764733807E-4 86-87 0.0 4.315623095056993E-4 0.0 5.589244388341344 3.686261393694515E-4 88 0.0 4.315623095056993E-4 0.0 5.889081293751877 3.776170208174869E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8440 0.0 48.415794 1 GTATCAA 14745 0.0 38.045685 1 TACCTGG 4550 0.0 34.190662 2 ATCAACG 17240 0.0 31.517696 3 CAACGCA 17365 0.0 31.334526 5 GTACTAG 3090 0.0 31.196445 1 GTACCTG 5425 0.0 29.903986 1 TATCAAC 18740 0.0 29.744455 2 TCAACGC 18965 0.0 28.82443 4 AACGCAG 19330 0.0 28.659678 6 ACCTGGG 5535 0.0 27.259712 3 GTACATG 18070 0.0 27.193672 1 TACATGG 18200 0.0 26.391888 2 ACGCAGA 21215 0.0 25.736677 7 CTGTGCG 4060 0.0 25.69193 9 ACATGGG 18345 0.0 25.391586 3 TAGTACT 4090 0.0 25.168348 4 CGCAGAG 21660 0.0 25.05494 8 GTGGTAT 3725 0.0 24.74227 1 CTAGTAC 4345 0.0 24.12398 3 >>END_MODULE