FastQCFastQC Report
Mon 6 Jun 2016
ERR1211186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8202956
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249400.30403673992643626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194030.23653668263001779No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124140.15133568923202806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125050.046.4710851
GTATCAA225250.040.776931
CAACGCA262850.033.4615945
ATCAACG263900.033.3638763
GTACTAG55850.032.7737351
TATCAAC285400.031.9026322
AACGCAG292600.030.814126
GCATCGT51550.030.0906528
TCAACGC301850.029.4805034
GTGGTAT49150.027.667751
GTACATG300750.027.364261
ACGCAGA322300.027.303677
CGCAGAG326800.026.8395928
TACATGG306550.026.432082
CTGTGCG72000.025.9834589
ACATGGG302050.025.7882443
TACCTGG53900.025.7532922
TAGTACT77000.025.7019964
CTAACGC27200.025.5782573
TAGGCAT72650.024.9118465
Produced by FastQC (version 0.11.5)