##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211186_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8202956 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.503947845142655 31.0 31.0 34.0 30.0 34.0 2 31.527722811142716 31.0 31.0 34.0 28.0 34.0 3 31.594582733346368 33.0 31.0 34.0 28.0 34.0 4 35.18855202929286 37.0 35.0 37.0 32.0 37.0 5 35.24265264863057 37.0 35.0 37.0 32.0 37.0 6 35.30356873790375 37.0 35.0 37.0 32.0 37.0 7 35.22069239430274 37.0 35.0 37.0 32.0 37.0 8 35.22751822635645 37.0 35.0 37.0 32.0 37.0 9 36.805274952102636 39.0 37.0 39.0 32.0 39.0 10-11 36.75741494895255 39.0 37.0 39.0 32.0 39.0 12-13 36.65658842982944 39.0 37.0 39.0 32.0 39.0 14-15 37.86535682990376 40.0 38.0 41.0 32.0 41.0 16-17 37.841452703147496 40.0 38.0 41.0 32.0 41.0 18-19 37.850840050830456 40.0 38.0 41.0 32.0 41.0 20-21 37.81645604340679 40.0 37.5 41.0 32.0 41.0 22-23 37.70973433235531 40.0 37.0 41.0 32.0 41.0 24-25 37.618243716045775 40.0 37.0 41.0 32.0 41.0 26-27 37.47240762963985 40.0 37.0 41.0 31.5 41.0 28-29 37.354608021791165 40.0 37.0 41.0 31.0 41.0 30-31 37.20667939703687 40.0 36.0 41.0 31.0 41.0 32-33 37.02879516847341 39.0 36.0 41.0 30.5 41.0 34-35 36.87897521332554 39.0 36.0 41.0 30.0 41.0 36-37 36.769706920285806 39.0 35.5 41.0 30.0 41.0 38-39 36.628945955579916 39.0 35.0 41.0 30.0 41.0 40-41 36.46528044280623 39.0 35.0 41.0 29.5 41.0 42-43 36.2618419506334 39.0 35.0 40.0 29.0 41.0 44-45 36.04656674983994 38.5 35.0 40.0 28.0 41.0 46-47 35.81735011135011 38.0 35.0 40.0 27.0 41.0 48-49 35.61651628998132 38.0 34.0 40.0 27.0 41.0 50-51 34.94401603519512 37.5 33.5 39.5 26.0 40.5 52-53 35.056261303851926 38.0 33.5 40.0 26.0 40.5 54-55 35.54216748206378 38.0 34.0 40.0 27.0 41.0 56-57 35.502464355532325 38.0 34.0 40.0 27.0 41.0 58-59 35.29011096731471 38.0 34.0 40.0 26.0 41.0 60-61 35.065685894206915 37.5 34.0 40.0 26.0 41.0 62-63 34.77301767801754 37.0 34.0 40.0 26.0 41.0 64-65 34.523254178127985 36.5 33.0 40.0 26.0 41.0 66-67 34.3487086850155 36.0 33.0 39.5 26.0 41.0 68-69 34.05277365866646 35.5 33.0 39.0 25.5 41.0 70-71 33.746856803815604 35.0 33.0 39.0 25.0 41.0 72-73 33.40999226376443 35.0 33.0 38.0 25.0 40.0 74-75 33.07421147937402 35.0 32.0 37.0 24.0 40.0 76-77 32.74311004472047 35.0 32.0 37.0 24.0 39.0 78-79 32.41008192656403 35.0 32.0 36.0 23.0 39.0 80-81 32.107475707537624 35.0 31.0 36.0 23.0 38.5 82-83 31.816409231013793 35.0 31.0 35.5 22.0 37.0 84-85 31.566765322159476 35.0 31.0 35.0 21.0 37.0 86-87 31.33904893553007 34.5 31.0 35.0 20.0 36.5 88-89 31.124827562161737 34.0 31.0 35.0 20.0 36.0 90-91 30.902076336871733 34.0 30.5 35.0 20.0 36.0 92-93 30.706706216637027 34.0 30.0 35.0 19.0 35.5 94-95 30.534723275365614 34.0 30.0 35.0 18.5 35.0 96-97 30.357543061306192 34.0 29.5 35.0 18.0 35.0 98-99 30.127059501477273 34.0 29.0 35.0 18.0 35.0 100 28.99592854088209 33.0 27.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.42526421593103336 1101 2 -0.36833465398234466 1101 3 -0.37676831553644874 1101 4 -0.3166421653000171 1101 5 -0.23699945053587612 1101 6 -0.27838583452573573 1101 7 -0.22549943478684753 1101 8 -0.21075739113997116 1101 9 -0.3929352799590262 1101 10-11 -0.38910625712786384 1101 12-13 -0.4173981453923403 1101 14-15 -0.5835612004340334 1101 16-17 -0.5467212885172117 1101 18-19 -0.5462317709525806 1101 20-21 -0.6267200675444613 1101 22-23 -0.6708467635197835 1101 24-25 -0.7401518188889682 1101 26-27 -0.7982815215156691 1101 28-29 -0.7153845781733423 1101 30-31 -0.8007887233262423 1101 32-33 -0.7814053236292509 1101 34-35 -0.8560758779855675 1101 36-37 -0.8673238760724828 1101 38-39 -0.9331852718352422 1101 40-41 -0.8508433189424451 1101 42-43 -0.8906959695313219 1101 44-45 -0.8597767608074847 1101 46-47 -1.0281266086298828 1101 48-49 -1.128765431281174 1101 50-51 -1.1791632340173308 1101 52-53 -0.9479763015953395 1101 54-55 -0.8262625588305497 1101 56-57 -0.8284077834179229 1101 58-59 -0.8018972888838292 1101 60-61 -0.7946387895923905 1101 62-63 -0.8423711850168942 1101 64-65 -0.8183984823439161 1101 66-67 -0.8764195022301138 1101 68-69 -0.8619459566743188 1101 70-71 -0.8620595452792514 1101 72-73 -0.9114999015745937 1101 74-75 -0.9274225422261893 1101 76-77 -0.9976713546022182 1101 78-79 -1.0403350950246448 1101 80-81 -1.1524639471720945 1101 82-83 -1.1118664751544962 1101 84-85 -1.114752172712791 1101 86-87 -1.1515096614177978 1101 88-89 -1.1855000848707817 1101 90-91 -1.248990787944603 1101 92-93 -1.3961492319744373 1101 94-95 -1.4309350915332466 1101 96-97 -1.537625444031459 1101 98-99 -1.5466148279472485 1101 100 -1.6662516264467797 1102 1 -0.34967597753367485 1102 2 -0.35541798142865133 1102 3 -0.3423121969447642 1102 4 -0.20085825554341596 1102 5 -0.24459793561419474 1102 6 -0.2356369812004715 1102 7 -0.27805191761105164 1102 8 -0.30814718986853507 1102 9 -0.3877746275731795 1102 10-11 -0.4435051406476589 1102 12-13 -0.44323160915304527 1102 14-15 -0.48755357588173354 1102 16-17 -0.5797962734308939 1102 18-19 -0.5917654540174624 1102 20-21 -0.6378795484387823 1102 22-23 -0.5680685436823509 1102 24-25 -0.6521730623588837 1102 26-27 -0.72979506717855 1102 28-29 -0.7415922197168001 1102 30-31 -0.7633917002538624 1102 32-33 -0.7823284190145117 1102 34-35 -0.8425863289875366 1102 36-37 -0.7821688310596144 1102 38-39 -0.891854882345342 1102 40-41 -0.9656997613956051 1102 42-43 -0.9438182346865602 1102 44-45 -1.1144927735976538 1102 46-47 -1.064302698249712 1102 48-49 -1.0502746408044175 1102 50-51 -1.025599452586654 1102 52-53 -0.9902139416897668 1102 54-55 -0.7896513218799939 1102 56-57 -0.8830003821602119 1102 58-59 -0.8652361335722318 1102 60-61 -0.77787392544478 1102 62-63 -0.8924931003821897 1102 64-65 -0.8464622267730064 1102 66-67 -0.8114642708548772 1102 68-69 -0.8726955907748888 1102 70-71 -0.889652436857908 1102 72-73 -0.9213783648046459 1102 74-75 -1.0130719608267889 1102 76-77 -1.0773207946699372 1102 78-79 -1.1342941336378303 1102 80-81 -1.1183701136504283 1102 82-83 -1.0183609660082062 1102 84-85 -1.0271111491443357 1102 86-87 -1.1142008981551434 1102 88-89 -1.1999198210712478 1102 90-91 -1.104266833414453 1102 92-93 -1.1029675131129117 1102 94-95 -1.1757879291162467 1102 96-97 -1.2978412477544943 1102 98-99 -1.3425513298789404 1102 100 -1.2763407431159521 1103 1 -0.48882369526980796 1103 2 -0.4413553905477343 1103 3 -0.5346295086478179 1103 4 -0.3767056603583825 1103 5 -0.2896540969040373 1103 6 -0.45347406942163815 1103 7 -0.36263203324065785 1103 8 -0.42646687133041183 1103 9 -0.5439639191216514 1103 10-11 -0.51563259744443 1103 12-13 -0.5608691124974712 1103 14-15 -0.6950144524266051 1103 16-17 -0.808718548332898 1103 18-19 -0.6778329007435957 1103 20-21 -0.7098740594392083 1103 22-23 -0.6497369386443737 1103 24-25 -0.703295706517828 1103 26-27 -0.7474195252167064 1103 28-29 -0.8068519874937294 1103 30-31 -0.8554252973184191 1103 32-33 -0.8700585191060739 1103 34-35 -0.8899345588973517 1103 36-37 -0.8417636538179423 1103 38-39 -0.8181584574239835 1103 40-41 -0.9134279670095751 1103 42-43 -0.9588737229383071 1103 44-45 -1.0621208544087537 1103 46-47 -1.0468907457578993 1103 48-49 -1.0931266023365467 1103 50-51 -1.1041623168373675 1103 52-53 -1.0144553612849805 1103 54-55 -0.7863054500962434 1103 56-57 -0.7732519654615473 1103 58-59 -0.8025754561136935 1103 60-61 -0.7814755101586712 1103 62-63 -0.7528692705129245 1103 64-65 -0.7129219134530018 1103 66-67 -0.8210259818008083 1103 68-69 -0.7909046364068715 1103 70-71 -0.6960200004101509 1103 72-73 -0.8244425992279716 1103 74-75 -0.7821536294294802 1103 76-77 -0.8151232255838217 1103 78-79 -0.8404294990476586 1103 80-81 -0.8837390631626008 1103 82-83 -0.9004189336990542 1103 84-85 -0.8846171441688391 1103 86-87 -1.014949590716327 1103 88-89 -1.0136552822020484 1103 90-91 -1.092432372534585 1103 92-93 -1.1565536013324156 1103 94-95 -1.1820889366792038 1103 96-97 -1.2309028315710044 1103 98-99 -1.2677207111325863 1103 100 -1.3454114852360064 1104 1 -0.46668943337754953 1104 2 -0.4701569510264534 1104 3 -0.485473956869253 1104 4 -0.37469964186710314 1104 5 -0.32512636978879783 1104 6 -0.3848188528511045 1104 7 -0.4334772877045907 1104 8 -0.33122766063454634 1104 9 -0.48758058495302237 1104 10-11 -0.42905106925899616 1104 12-13 -0.46382493549310055 1104 14-15 -0.6573750759789121 1104 16-17 -0.7531167246268922 1104 18-19 -0.8295097035490215 1104 20-21 -0.7260532217750466 1104 22-23 -0.7352738017218812 1104 24-25 -0.7470505594076258 1104 26-27 -0.7545367216428005 1104 28-29 -0.8084345107712565 1104 30-31 -0.7404844798756898 1104 32-33 -0.9256929920014372 1104 34-35 -1.0094762427239132 1104 36-37 -0.8940654943025024 1104 38-39 -0.834510907420615 1104 40-41 -0.914588665665903 1104 42-43 -0.923187850267972 1104 44-45 -0.9713601391917663 1104 46-47 -0.972556513209959 1104 48-49 -0.9343042938245816 1104 50-51 -0.9719748828893202 1104 52-53 -0.8076626956221205 1104 54-55 -0.6796313420292464 1104 56-57 -0.7051099476617466 1104 58-59 -0.7239226510072072 1104 60-61 -0.7285021459411638 1104 62-63 -0.7456648037842228 1104 64-65 -0.792382413744626 1104 66-67 -0.8047813213414869 1104 68-69 -0.9118186212277166 1104 70-71 -0.8351516881371808 1104 72-73 -0.7966842874852347 1104 74-75 -0.8019572512741533 1104 76-77 -0.735680829431125 1104 78-79 -0.8123066712719549 1104 80-81 -0.8945797561825017 1104 82-83 -0.8286054618953891 1104 84-85 -0.8285746290408973 1104 86-87 -0.8331613557554718 1104 88-89 -0.897802920456293 1104 90-91 -1.0449559297055302 1104 92-93 -1.0638334444710402 1104 94-95 -0.9784604641165053 1104 96-97 -1.118578307344869 1104 98-99 -1.0918004506944712 1104 100 -1.0786064862419948 1105 1 -0.4024443103812949 1105 2 -0.3834734214526563 1105 3 -0.4282139107755505 1105 4 -0.2782320061706258 1105 5 -0.2780505223072467 1105 6 -0.29733747570729463 1105 7 -0.29075125037149974 1105 8 -0.3930740626609719 1105 9 -0.538241370459076 1105 10-11 -0.5954011728421733 1105 12-13 -0.6005821473885646 1105 14-15 -0.7040572318754528 1105 16-17 -0.7067653480548515 1105 18-19 -0.7288689298594733 1105 20-21 -0.7429749297233528 1105 22-23 -0.686472622804736 1105 24-25 -0.753781380158209 1105 26-27 -0.7274798107696441 1105 28-29 -0.752864051736509 1105 30-31 -0.8198679936629887 1105 32-33 -0.869727906418106 1105 34-35 -0.8307599872674061 1105 36-37 -0.8408018601062821 1105 38-39 -0.8354329792855069 1105 40-41 -0.8543966097637181 1105 42-43 -0.9685052290472456 1105 44-45 -0.915644563022262 1105 46-47 -1.0182211410566921 1105 48-49 -1.0734047941379856 1105 50-51 -1.083772828630515 1105 52-53 -0.9950508820696768 1105 54-55 -0.8284556792749171 1105 56-57 -0.7356128645683029 1105 58-59 -0.6999893095948195 1105 60-61 -0.7369224638503482 1105 62-63 -0.765999740100618 1105 64-65 -0.7457891153477689 1105 66-67 -0.7168914010758911 1105 68-69 -0.799802775033541 1105 70-71 -0.7810316273070086 1105 72-73 -0.7076558692673984 1105 74-75 -0.6917341617733186 1105 76-77 -0.7666994147089738 1105 78-79 -0.8343178893964378 1105 80-81 -0.8569537653458639 1105 82-83 -1.0081142248414885 1105 84-85 -0.9598432631346334 1105 86-87 -0.9677277322358186 1105 88-89 -0.8982253801512741 1105 90-91 -1.0290177315169835 1105 92-93 -0.9796956229289755 1105 94-95 -1.107027143381206 1105 96-97 -1.039458525205763 1105 98-99 -0.9744658304676825 1105 100 -1.104249624310583 1106 1 -0.5209954866144741 1106 2 -0.4894237282813023 1106 3 -0.5565669051401052 1106 4 -0.41519588486019643 1106 5 -0.41255068100419834 1106 6 -0.36769264105311095 1106 7 -0.4481231730334727 1106 8 -0.439133501343143 1106 9 -0.5080726875530317 1106 10-11 -0.5457281461414709 1106 12-13 -0.6124174239670879 1106 14-15 -0.8124455914933932 1106 16-17 -0.7444413127866127 1106 18-19 -0.8847180248506419 1106 20-21 -0.7489365346581565 1106 22-23 -0.7272068321964227 1106 24-25 -0.7827001435468972 1106 26-27 -0.7744424610628116 1106 28-29 -0.8677319144960052 1106 30-31 -0.8633071528814824 1106 32-33 -0.9338584957744374 1106 34-35 -0.8870469514863473 1106 36-37 -0.9372644099815304 1106 38-39 -0.857811369349875 1106 40-41 -0.9101573788654989 1106 42-43 -0.9277914609395239 1106 44-45 -0.8939312344501289 1106 46-47 -0.9027467313980182 1106 48-49 -0.8419438521708145 1106 50-51 -0.8732958548688146 1106 52-53 -0.7753225827653054 1106 54-55 -0.7147783926211204 1106 56-57 -0.7036563675989029 1106 58-59 -0.6872424110776407 1106 60-61 -0.6506368117821211 1106 62-63 -0.6849097773381274 1106 64-65 -0.6556575268724316 1106 66-67 -0.7322682893209631 1106 68-69 -0.7501248539409104 1106 70-71 -0.6528743475489804 1106 72-73 -0.7129570540941117 1106 74-75 -0.6661255253788312 1106 76-77 -0.6792208832917765 1106 78-79 -0.6449079576369989 1106 80-81 -0.6374798261071675 1106 82-83 -0.672946317535164 1106 84-85 -0.6450965300321947 1106 86-87 -0.6721502646552757 1106 88-89 -0.7115812335037752 1106 90-91 -0.7698415580101816 1106 92-93 -0.8900420882968341 1106 94-95 -0.8882784378649937 1106 96-97 -0.9502751488291175 1106 98-99 -0.7926148464680729 1106 100 -0.9234732428837269 1107 1 -0.5590118092117109 1107 2 -0.5427404992889571 1107 3 -0.5038061124315476 1107 4 -0.4615576154539198 1107 5 -0.4031546976809608 1107 6 -0.4362101869282995 1107 7 -0.44413022232384236 1107 8 -0.4384406936010805 1107 9 -0.6231493827199444 1107 10-11 -0.5979950477500466 1107 12-13 -0.6967369798593808 1107 14-15 -0.7849107537478446 1107 16-17 -0.8077131187846049 1107 18-19 -0.8217845131643884 1107 20-21 -0.8166843266756771 1107 22-23 -0.8262838662086196 1107 24-25 -0.777763823818141 1107 26-27 -0.874508740783547 1107 28-29 -0.9260970755538409 1107 30-31 -0.8946941345939123 1107 32-33 -0.9843219760270969 1107 34-35 -0.9443564924172776 1107 36-37 -0.9299386111920924 1107 38-39 -0.9799926755071695 1107 40-41 -0.9678270806480782 1107 42-43 -0.940593493597973 1107 44-45 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1.186199428924379 2310 92-93 1.2252572683974527 2310 94-95 1.2633609270051807 2310 96-97 1.318687673676262 2310 98-99 1.3467818535359974 2310 100 1.3240896253766046 2311 1 0.7310564746941033 2311 2 0.6734798785259493 2311 3 0.6822903969116894 2311 4 0.5605956407226529 2311 5 0.5659170455438556 2311 6 0.6222533245473088 2311 7 0.6515180238207208 2311 8 0.6038325418915775 2311 9 0.7924376096389381 2311 10-11 0.7897445634682114 2311 12-13 0.8486730313126145 2311 14-15 1.0410606628111267 2311 16-17 1.0403503867076864 2311 18-19 1.0326874931825643 2311 20-21 1.0515476688924608 2311 22-23 1.0734702178333109 2311 24-25 1.0987850021164078 2311 26-27 1.1325314920968523 2311 28-29 1.1396588238328818 2311 30-31 1.1321182627821216 2311 32-33 1.1787434522000098 2311 34-35 1.1705311224332249 2311 36-37 1.2256394114457478 2311 38-39 1.2678407778334915 2311 40-41 1.2837727652234676 2311 42-43 1.2436180913963284 2311 44-45 1.2724231231533594 2311 46-47 1.3165456082178295 2311 48-49 1.362450499805476 2311 50-51 1.3328454141254014 2311 52-53 1.212623304187261 2311 54-55 1.183022160572385 2311 56-57 1.0850505619694601 2311 58-59 1.1047858811035738 2311 60-61 1.076387878936572 2311 62-63 1.0807950154455384 2311 64-65 1.0591186258240413 2311 66-67 1.0572450299979153 2311 68-69 1.0399317378985913 2311 70-71 1.0192308229105507 2311 72-73 1.0410147872044817 2311 74-75 1.0645622168821731 2311 76-77 1.1163961534398226 2311 78-79 1.1392002324570782 2311 80-81 1.0501496763094522 2311 82-83 1.1042518138582587 2311 84-85 1.141171097541413 2311 86-87 1.1713584280096754 2311 88-89 1.1522273705715662 2311 90-91 1.1972353629152188 2311 92-93 1.2871386934905402 2311 94-95 1.3327103761392003 2311 96-97 1.3661078017005508 2311 98-99 1.4498799465731445 2311 100 1.5057743297637671 2312 1 0.6852927040331842 2312 2 0.6426405641317992 2312 3 0.6395314646366188 2312 4 0.3755327611799899 2312 5 0.45443794893466816 2312 6 0.5340389784600887 2312 7 0.40708005286094817 2312 8 0.5757509435581483 2312 9 0.6859856446507848 2312 10-11 0.6802019176595167 2312 12-13 0.808347907831859 2312 14-15 1.0156929329247717 2312 16-17 1.0115736108119577 2312 18-19 0.9572316290494456 2312 20-21 0.994806710639871 2312 22-23 1.0770873362546425 2312 24-25 1.0618819485272937 2312 26-27 1.1037643116020845 2312 28-29 1.1108532128336108 2312 30-31 1.0924693433981432 2312 32-33 1.132514407827614 2312 34-35 1.1494492712941167 2312 36-37 1.162916247739254 2312 38-39 1.1749550906378303 2312 40-41 1.216778012636368 2312 42-43 1.265854634395268 2312 44-45 1.2620630295978756 2312 46-47 1.2380838353172052 2312 48-49 1.3112526025609839 2312 50-51 1.2535215004258546 2312 52-53 1.1499313662844415 2312 54-55 1.0975872044158734 2312 56-57 0.9810375783813541 2312 58-59 0.9893216938566312 2312 60-61 1.00755367820652 2312 62-63 1.0270732289127196 2312 64-65 1.0125856310978776 2312 66-67 1.014899156370241 2312 68-69 0.9985682518527028 2312 70-71 0.9799478925485587 2312 72-73 0.9907991743069928 2312 74-75 1.0279908403348657 2312 76-77 1.0228998977326214 2312 78-79 0.9924279036547929 2312 80-81 1.0471215883649023 2312 82-83 1.0848646268025384 2312 84-85 1.1328897883783746 2312 86-87 1.1412694837502357 2312 88-89 1.0586356389948577 2312 90-91 1.0735041618053067 2312 92-93 1.1608114800162745 2312 94-95 1.2379661478000408 2312 96-97 1.2193194063067416 2312 98-99 0.8678107321532913 2312 100 1.0916884311258883 2313 1 0.5562562380998202 2313 2 0.5363832793252676 2313 3 0.5595901172279198 2313 4 0.46059615819654454 2313 5 0.27850024741055535 2313 6 0.44631186232523135 2313 7 0.5133043441503702 2313 8 0.5165659612125495 2313 9 0.6592481340491574 2313 10-11 0.6749900683050924 2313 12-13 0.7458725473745034 2313 14-15 0.8889935981070138 2313 16-17 0.8842459221646735 2313 18-19 0.8581358288712764 2313 20-21 0.9051973807660403 2313 22-23 0.9262749524498659 2313 24-25 0.9013838103870668 2313 26-27 0.9133750617379022 2313 28-29 0.9620197971864997 2313 30-31 0.9373281888968492 2313 32-33 1.019433383623408 2313 34-35 1.014196077609327 2313 36-37 1.0282916575933783 2313 38-39 1.0905398594999625 2313 40-41 1.0450376284109737 2313 42-43 1.1193091092974896 2313 44-45 1.1312518790387074 2313 46-47 1.18418901012776 2313 48-49 1.1950285712881055 2313 50-51 1.21200436053811 2313 52-53 1.0790387220567865 2313 54-55 0.9789326241511631 2313 56-57 0.9768175587801196 2313 58-59 0.9149049222774934 2313 60-61 0.9651674858903618 2313 62-63 0.9799356619809103 2313 64-65 0.9871973693922058 2313 66-67 0.9748010777479692 2313 68-69 0.9577219588943038 2313 70-71 0.9635060700597862 2313 72-73 0.9705122735206615 2313 74-75 0.9347363324986304 2313 76-77 0.6816976902633698 2313 78-79 0.8312487655077305 2313 80-81 0.9426502304196305 2313 82-83 0.9703386140601573 2313 84-85 1.0058563370855147 2313 86-87 1.0423440916345044 2313 88-89 1.0654230430199831 2313 90-91 1.1323205943449786 2313 92-93 1.2484003474701808 2313 94-95 1.2273571912895669 2313 96-97 1.2397171993135352 2313 98-99 1.3256943428622243 2313 100 1.3872712091135107 2314 1 0.5005803952294201 2314 2 0.5582412028225328 2314 3 0.5421656404777409 2314 4 0.435851339143035 2314 5 0.4239791627626559 2314 6 0.5083801167844584 2314 7 0.5076336564506079 2314 8 0.5012035527168237 2314 9 0.6475974484114673 2314 10-11 0.6828259276720416 2314 12-13 0.7360777231658204 2314 14-15 0.8824465313991112 2314 16-17 0.8680586863671707 2314 18-19 0.8347829058895258 2314 20-21 0.9078265177904754 2314 22-23 0.9225632295918373 2314 24-25 0.9575236186147222 2314 26-27 0.9471414294046738 2314 28-29 0.9456263545635437 2314 30-31 0.9697026604918619 2314 32-33 0.9615408173794648 2314 34-35 0.9433124813622911 2314 36-37 0.964779955356029 2314 38-39 0.9393387047417434 2314 40-41 1.0334900461860101 2314 42-43 1.117968764932094 2314 44-45 1.1321798097532039 2314 46-47 1.1330497174171725 2314 48-49 1.114865667895998 2314 50-51 1.1051892249571225 2314 52-53 0.9759868610401838 2314 54-55 0.8912947233366495 2314 56-57 0.8380403652550186 2314 58-59 0.8302054687255804 2314 60-61 0.8110278838695422 2314 62-63 0.8409007099239929 2314 64-65 0.9050755011583647 2314 66-67 0.8976797250311961 2314 68-69 0.8791571583994084 2314 70-71 0.9111810881028788 2314 72-73 0.9492214187933783 2314 74-75 0.9320040920614687 2314 76-77 0.923217434566908 2314 78-79 0.8880587459180802 2314 80-81 0.8491354350799085 2314 82-83 0.9156422536106241 2314 84-85 0.9758016922767716 2314 86-87 1.0389932307210117 2314 88-89 1.0500606345242574 2314 90-91 1.086130265445096 2314 92-93 1.1378734890311684 2314 94-95 1.1567313535743402 2314 96-97 1.100321385312089 2314 98-99 1.17387456762766 2314 100 1.2244709204239577 2315 1 0.33024139032120203 2315 2 0.32692080678122437 2315 3 0.4151337127031063 2315 4 0.15447756316336836 2315 5 0.2375706639996764 2315 6 0.30813887254235794 2315 7 0.360012477780252 2315 8 0.3977411474719972 2315 9 0.5133084218527983 2315 10-11 0.40962298625271387 2315 12-13 0.45515344828160664 2315 14-15 0.6383254379300709 2315 16-17 0.5655616224697297 2315 18-19 0.4806561268407705 2315 20-21 0.5956091214247792 2315 22-23 0.436965673706716 2315 24-25 0.6048192325752595 2315 26-27 0.6783656927618438 2315 28-29 0.6901295548567248 2315 30-31 0.7034514524170703 2315 32-33 0.6988583530139891 2315 34-35 0.7595937808049555 2315 36-37 0.7552823123177959 2315 38-39 0.7533036944475597 2315 40-41 0.7814391786249715 2315 42-43 0.8481727512636326 2315 44-45 0.8378416509646982 2315 46-47 0.9183063476414972 2315 48-49 0.9204683339588797 2315 50-51 0.8168489345198182 2315 52-53 0.8205848600735308 2315 54-55 0.7788004718113442 2315 56-57 0.772867332443731 2315 58-59 0.7027212990917562 2315 60-61 0.6527625769258663 2315 62-63 0.6704364853823748 2315 64-65 0.7250473261071448 2315 66-67 0.7745735214819902 2315 68-69 0.7398590613386062 2315 70-71 0.72970933853302 2315 72-73 0.5135147139727465 2315 74-75 0.675771848804338 2315 76-77 0.7201415593903491 2315 78-79 0.7636277395596949 2315 80-81 0.5196757356184349 2315 82-83 0.4777779657114216 2315 84-85 0.7813288685176438 2315 86-87 0.8835908653753677 2315 88-89 0.9190758442104325 2315 90-91 0.991241967251522 2315 92-93 1.0809497431618773 2315 94-95 0.982495442066341 2315 96-97 0.8879720013692385 2315 98-99 0.34626608772367007 2315 100 0.901164346450237 2316 1 0.2327212700920711 2316 2 0.19020144594423982 2316 3 0.20582223130727684 2316 4 0.12708159555879206 2316 5 0.04080157375802429 2316 6 0.0773596355017716 2316 7 0.06225862545397831 2316 8 0.16224822204116407 2316 9 0.19222527528587818 2316 10-11 0.13834303365378275 2316 12-13 -0.05846488767880942 2316 14-15 -0.051910361775419744 2316 16-17 0.12002961038273696 2316 18-19 0.22076053823489872 2316 20-21 0.18625287326126028 2316 22-23 0.16260161719610977 2316 24-25 0.2534825655611641 2316 26-27 0.32570100675213354 2316 28-29 0.2669102472020697 2316 30-31 0.13481638931040862 2316 32-33 0.3459414828691578 2316 34-35 0.3218314149081394 2316 36-37 0.34680442074301965 2316 38-39 0.4727539801525822 2316 40-41 0.5235039384302596 2316 42-43 0.43946201029902454 2316 44-45 0.3878676892593731 2316 46-47 0.2249506645363084 2316 48-49 0.20194437260222742 2316 50-51 0.2550031313595227 2316 52-53 0.35659140178277227 2316 54-55 0.06304927941827998 2316 56-57 -0.0696524551209805 2316 58-59 -0.02955131535092903 2316 60-61 0.046455199773433264 2316 62-63 0.1542576542866314 2316 64-65 0.09980989826217979 2316 66-67 0.11938911764459448 2316 68-69 0.08840408013335832 2316 70-71 -0.04275631614270736 2316 72-73 -0.16357545366687987 2316 74-75 0.08952712062447432 2316 76-77 0.21337359750621943 2316 78-79 0.1805633649414844 2316 80-81 0.37476689289545106 2316 82-83 0.4381055500129527 2316 84-85 0.5120627293769573 2316 86-87 0.5226502015882772 2316 88-89 0.47674658320481456 2316 90-91 0.3269323960845476 2316 92-93 0.38656140246249393 2316 94-95 0.5574412969262674 2316 96-97 0.47074904062571576 2316 98-99 0.3081395877887907 2316 100 0.2397874524854977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 31.0 1 499.0 2 205.0 3 146.0 4 168.0 5 111.0 6 135.0 7 539.0 8 1881.0 9 3635.0 10 5711.0 11 8932.0 12 12677.0 13 16263.0 14 18766.0 15 21767.0 16 25639.0 17 31530.0 18 38672.0 19 47112.0 20 57635.0 21 70164.0 22 78898.0 23 79734.0 24 79516.0 25 82206.0 26 89834.0 27 103839.0 28 123004.0 29 147167.0 30 180610.0 31 225165.0 32 288315.0 33 381523.0 34 539294.0 35 759262.0 36 1148764.0 37 1629712.0 38 1481638.0 39 421775.0 40 482.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67491324690938 19.83203279424786 13.011542561373032 23.481511397469735 2 15.663558150827347 19.59802908426341 41.30758096532685 23.430831799582393 3 19.512540684402673 25.456919951433548 28.755871149171142 26.274668214992637 4 12.669471402243829 16.056540152811554 38.467787707406046 32.80620073753857 5 13.423301360579554 37.65415434858471 34.60281821620273 14.319726074633005 6 30.913586318361265 37.18172722468177 17.718136375096098 14.186550081860869 7 27.67103072863486 31.86802450132949 22.37925439932356 18.081690370712085 8 25.972466055804084 35.74443785006229 19.76908360123519 18.514012492898434 9 26.022115867868724 15.914004931684136 21.150178306760804 36.91370089368634 10-11 25.652678649937457 26.139695933111774 27.427320896387908 20.780304520562865 12-13 25.6784678305018 23.735272936595194 28.059567430909865 22.52669180199314 14-15 22.919623478918233 25.110572585168384 25.81381837247299 26.15598556344039 16-17 22.045194005086397 27.958537026095026 26.81686587040446 23.179403098414117 18-19 21.626283096158208 27.509170731522936 29.050577885178193 21.81396828714066 20-21 22.749301597896217 26.75004124385869 28.768387048708966 21.732270109536127 22-23 22.860122861647096 26.547301404896967 28.501013400988107 22.091562332467834 24-25 22.3952940462061 26.508022473791236 29.0309861789464 22.065697301056264 26-27 22.361371349337116 26.812222324779828 28.42145680109123 22.404949524791824 28-29 21.884047580465513 27.04777323670144 28.77336801995456 22.294811162878485 30-31 22.484736200702425 26.80560305647865 28.69416863427175 22.01549210854718 32-33 22.579035810122615 26.996573979878946 28.193254506947333 22.231135703051105 34-35 22.646361642171957 26.885273786777127 28.611489448353815 21.856875122697105 36-37 22.432340750139705 26.679694658945873 28.36461120447981 22.523353386434604 38-39 22.502711786369762 26.86234733398722 28.626264382588186 22.008676497054836 40-41 22.410155171141863 26.30063974334665 28.90124857562147 22.38795650989002 42-43 22.3275855919103 26.469298297761988 28.506351221651897 22.69676488867581 44-45 22.414189464004668 27.026905564353402 28.377909667599845 22.180995304042085 46-47 22.40613836831454 26.894457049460325 27.928367164415384 22.771037417809747 48-49 22.79934341368176 26.813906902626183 27.93759051797548 22.44915916571658 50-51 22.578012599372503 26.857211806410632 27.87551566818543 22.68925992603144 52-53 22.321904038412395 27.233835901808195 27.67584891322436 22.768411146555056 54-55 22.240916817135357 27.139864784277535 27.72036945663221 22.8988489419549 56-57 22.789820596069962 26.917772614367983 27.4426090660221 22.849797723539954 58-59 22.262248936962955 27.085250906013076 27.954511222488026 22.697988934535942 60-61 22.29795776797646 27.49352933584187 27.556033515946094 22.652479380235572 62-63 22.477395580691223 27.103031034663566 27.36682750404532 23.05274588059989 64-65 22.099039627337508 27.123738808974014 27.595554086336353 23.18166747735212 66-67 22.359142101542815 26.901594814674034 27.515889825664186 23.22337325811896 68-69 22.124935333362767 26.966018215686194 27.683930697365504 23.225115753585538 70-71 22.17190478228901 26.705742920180708 27.71455931618203 23.40779298134826 72-73 22.51360833212947 26.797079481148167 27.740416510123765 22.948895676598596 74-75 22.644926752014683 26.997108491766745 27.466395552838996 22.891569203379575 76-77 22.47643366746266 26.928667289212864 27.269937791917602 23.32496125140687 78-79 22.363789498583916 26.96258438806668 27.32516615460494 23.348459958744456 80-81 22.173527297948308 27.22336545076629 27.550547217357607 23.052560033927794 82-83 22.684569764994706 26.6738521461697 28.14444289183787 22.497135196997732 84-85 22.76582062067703 26.88981093828459 27.472163914244973 22.8722045267934 86-87 22.101586087754654 27.28461220424463 27.69815184631871 22.91564986168201 88-89 22.681616900462206 26.551276255702895 27.61571225514567 23.151394588689232 90-91 22.578443874284954 26.649152186493364 27.603388961682988 23.16901497753869 92-93 22.735406100809406 26.72061558735904 27.580158261981154 22.963820049850405 94-95 22.062329724152978 26.71983559140308 27.976153242196013 23.241681442247934 96-97 22.3463787970518 26.611429062661756 27.675807697536925 23.366384442749517 98-99 22.04959851544181 26.89613641000165 27.512133431715313 23.54213164284123 100 22.586288787960267 26.48045884923081 27.064629477538908 23.86862288527001 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1283.0 1 921.5 2 1056.0 3 1581.5 4 1224.5 5 997.0 6 1495.0 7 2720.0 8 4461.5 9 5348.5 10 4969.0 11 4506.5 12 4857.5 13 5607.0 14 6345.5 15 7322.0 16 8241.0 17 9016.5 18 9906.0 19 10827.0 20 11747.0 21 12924.0 22 14780.0 23 17332.5 24 20891.5 25 25540.5 26 31646.5 27 39475.5 28 49089.5 29 62919.5 30 81174.5 31 99330.0 32 121919.5 33 145769.5 34 165678.5 35 185577.5 36 204765.5 37 232659.5 38 249029.0 39 247569.5 40 243397.0 41 237024.5 42 238056.5 43 248697.5 44 264797.0 45 292693.0 46 317953.0 47 335027.0 48 349494.0 49 349073.0 50 339316.5 51 342498.0 52 340213.0 53 322995.0 54 304566.0 55 270769.5 56 239645.5 57 204195.0 58 169714.5 59 154395.0 60 129200.5 61 98247.0 62 76463.5 63 61577.0 64 46138.5 65 29867.0 66 20738.0 67 15307.0 68 11663.0 69 9001.5 70 7068.0 71 5314.5 72 4008.0 73 3071.0 74 2377.0 75 1801.0 76 1360.0 77 953.5 78 633.0 79 435.5 80 288.0 81 228.0 82 191.5 83 138.0 84 95.0 85 73.0 86 58.5 87 57.0 88 53.0 89 41.5 90 26.0 91 17.0 92 16.5 93 12.5 94 10.5 95 13.0 96 11.5 97 7.5 98 4.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05716232050982597 2 0.1294899058339457 3 0.017188925553178636 4 0.016469672639960523 5 0.019480782293602453 6 0.05228602957275402 7 0.05738175360199421 8 0.04946997155659496 9 0.04050978695972525 10-11 0.03958329168168158 12-13 0.040729220051893486 14-15 0.018968771745209896 16-17 0.017920369193739426 18-19 0.01775579437461325 20-21 0.021150911939549596 22-23 0.025667576420012495 24-25 0.024692318232598105 26-27 0.019541735930315853 28-29 0.01856647774290146 30-31 0.01659157991338732 32-33 0.023101428314378378 34-35 0.02274789722144066 36-37 0.023125809769063737 38-39 0.0209680510294094 40-41 0.024515552686129243 42-43 0.031750749364009755 44-45 0.040851127325320286 46-47 0.04680020226854807 48-49 0.03989415522891992 50-51 0.02815448479791919 52-53 0.040357402867941754 54-55 0.0368403780295786 56-57 0.03856536594856781 58-59 0.04353308734070986 60-61 0.023217240224133837 62-63 0.025917486330537434 64-65 0.025320140690746118 66-67 0.03244562082254251 68-69 0.039132234770002425 70-71 0.04080236441594957 72-73 0.044611966710537035 74-75 0.027045128609735323 76-77 0.020608424572800342 78-79 0.01991355311426759 80-81 0.024503361958786562 82-83 0.02065109211849972 84-85 0.012891694164883977 86-87 0.014665444993243898 88-89 0.0185725731065728 90-91 0.021236247030948355 92-93 0.01972459684045605 94-95 0.023467150134658773 96-97 0.024637459959556043 98-99 0.02763637888585529 100 0.02975756544348159 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8202956.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.86690836762134 #Duplication Level Percentage of deduplicated Percentage of total 1 76.13350905573549 48.624118465681285 2 14.399034432278576 18.39243625337121 3 4.69469366386327 8.99506710132024 4 1.9330738336366529 4.938377976028745 5 0.9098686596640507 2.905524915666719 6 0.541554773892071 2.0752457472127674 7 0.3436244134623467 1.5362360249234126 8 0.22332400431470145 1.141041097588585 9 0.15487724492406946 0.8902377728815674 >10 0.6286175175081389 6.681917075341157 >50 0.02600104729848693 1.136642848071118 >100 0.01089087570942044 1.2121985997259326 >500 5.387175834444295E-4 0.21506735441261549 >1k 3.1830510565429267E-4 0.36833315675142764 >5k 0.0 0.0 >10k+ 7.345502368429202E-5 0.8875556110232687 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24940 0.30403673992643626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19403 0.23653668263001779 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12414 0.15133568923202806 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.2190727342679883E-5 2.4381454685359766E-5 8 0.0 0.0 0.0 1.2190727342679883E-5 2.4381454685359766E-5 9 1.2190727342679883E-5 0.0 0.0 2.4381454685359766E-5 4.876290937071953E-5 10-11 3.657218202803965E-5 0.0 0.0 3.0476818356699708E-5 4.876290937071953E-5 12-13 3.657218202803965E-5 0.0 0.0 4.876290937071953E-5 4.876290937071953E-5 14-15 4.2667545699379594E-5 6.095363671339942E-6 0.0 4.876290937071953E-5 9.752581874143907E-5 16-17 5.485827304205947E-5 1.2190727342679883E-5 0.0 6.0953636713399416E-5 1.0971654608411894E-4 18-19 6.0953636713399416E-5 1.2190727342679883E-5 0.0 8.533509139875918E-5 1.2190727342679883E-4 20-21 7.31443640560793E-5 1.2190727342679883E-5 0.0 1.03621182412779E-4 1.2190727342679883E-4 22-23 7.923972772741924E-5 1.2190727342679883E-5 0.0 1.889562738115382E-4 1.645748191261784E-4 24-25 9.752581874143907E-5 1.2190727342679883E-5 0.0 5.120105483925551E-4 2.1943309216823788E-4 26-27 9.752581874143907E-5 1.2190727342679883E-5 0.0 0.0012068820069253083 2.1943309216823788E-4 28-29 9.752581874143907E-5 1.2190727342679883E-5 0.0 0.003212256654796149 2.1943309216823788E-4 30-31 9.752581874143907E-5 1.2190727342679883E-5 0.0 0.011709193612644027 2.1943309216823788E-4 32-33 1.0971654608411894E-4 1.2190727342679883E-5 0.0 0.03430470674230119 2.1943309216823788E-4 34-35 1.1581190975545888E-4 1.8286091014019825E-5 0.0 0.06851798302953228 2.3162381951091776E-4 36-37 1.2800263709813878E-4 3.0476818356699708E-5 0.0 0.1260033578139393 2.3162381951091776E-4 38-39 1.3409800076947872E-4 3.657218202803965E-5 0.0 0.20972317783979338 2.4381454685359764E-4 40-41 1.3409800076947872E-4 3.657218202803965E-5 0.0 0.29669182670247163 2.621006378676175E-4 42-43 1.3409800076947872E-4 4.876290937071953E-5 0.0 0.3831545603804287 2.6819600153895744E-4 44-45 1.5847945545483847E-4 4.876290937071953E-5 0.0 0.48637954415457063 2.6819600153895744E-4 46-47 1.8286091014019825E-4 4.876290937071953E-5 0.0 0.6101154266827714 2.803867288816373E-4 48-49 1.9505163748287813E-4 4.876290937071953E-5 0.0 0.7429516871722828 2.925774562243172E-4 50-51 1.9505163748287813E-4 4.876290937071953E-5 0.0 0.8824806569729253 2.925774562243172E-4 52-53 2.07242364825558E-4 4.876290937071953E-5 0.0 1.0146647135496034 3.1695891090967695E-4 54-55 2.07242364825558E-4 4.876290937071953E-5 0.0 1.1602037119301871 3.413403655950367E-4 56-57 2.3162381951091776E-4 4.876290937071953E-5 0.0 1.3371277378545003 3.413403655950367E-4 58-59 2.560052741962775E-4 5.485827304205947E-5 0.0 1.531167544967936 3.413403655950367E-4 60-61 2.803867288816373E-4 6.0953636713399416E-5 0.0 1.726395947997283 3.413403655950367E-4 62-63 2.803867288816373E-4 6.0953636713399416E-5 0.0 1.9251840434106924 3.657218202803965E-4 64-65 2.803867288816373E-4 6.0953636713399416E-5 0.0 2.130646562044219 3.657218202803965E-4 66-67 2.925774562243172E-4 9.752581874143907E-5 0.0 2.3607709220919872 3.779125476230764E-4 68-69 3.291496382523568E-4 9.752581874143907E-5 0.0 2.6142527157283304 3.9619863863709617E-4 70-71 3.291496382523568E-4 1.03621182412779E-4 0.0 2.8848685766448097 4.14484729651116E-4 72-73 3.291496382523568E-4 1.0971654608411894E-4 0.0 3.156118355383108 4.5105691167915564E-4 74-75 3.291496382523568E-4 1.0971654608411894E-4 0.0 3.4267464070269305 4.5105691167915564E-4 76-77 3.291496382523568E-4 1.0971654608411894E-4 0.0 3.726827255930667 4.5105691167915564E-4 78-79 3.291496382523568E-4 1.0971654608411894E-4 0.0 4.056013466365052 4.5105691167915564E-4 80-81 3.413403655950367E-4 1.0971654608411894E-4 0.0 4.403132236720518 4.571522753504956E-4 82-83 3.535310929377166E-4 1.0971654608411894E-4 0.0 4.757333819662083 4.7543836636451545E-4 84-85 3.535310929377166E-4 1.0971654608411894E-4 0.0 5.104141726470312 4.7543836636451545E-4 86-87 3.779125476230764E-4 1.2190727342679883E-4 0.0 5.470430415572142 4.7543836636451545E-4 88 3.779125476230764E-4 1.2190727342679883E-4 0.0 5.76300787179646 4.7543836636451545E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12505 0.0 46.471085 1 GTATCAA 22525 0.0 40.77693 1 CAACGCA 26285 0.0 33.461594 5 ATCAACG 26390 0.0 33.363876 3 GTACTAG 5585 0.0 32.773735 1 TATCAAC 28540 0.0 31.902632 2 AACGCAG 29260 0.0 30.81412 6 GCATCGT 5155 0.0 30.090652 8 TCAACGC 30185 0.0 29.480503 4 GTGGTAT 4915 0.0 27.66775 1 GTACATG 30075 0.0 27.36426 1 ACGCAGA 32230 0.0 27.30367 7 CGCAGAG 32680 0.0 26.839592 8 TACATGG 30655 0.0 26.43208 2 CTGTGCG 7200 0.0 25.983458 9 ACATGGG 30205 0.0 25.788244 3 TACCTGG 5390 0.0 25.753292 2 TAGTACT 7700 0.0 25.701996 4 CTAACGC 2720 0.0 25.578257 3 TAGGCAT 7265 0.0 24.911846 5 >>END_MODULE