FastQCFastQC Report
Mon 6 Jun 2016
ERR1211186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8202956
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222730.271524070103509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188650.229978071319656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120230.14656911484104024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130950.048.04341
GTATCAA234800.040.191331
CAACGCA276400.032.758365
ATCAACG276500.032.70753
GTACTAG53600.031.492911
TATCAAC302850.031.1160532
AACGCAG312950.029.6981836
TCAACGC312100.029.1574134
GTGGTAT52150.028.9423521
CTGTGCG73950.027.4485119
ACGCAGA338700.026.8438197
TAGTACT79100.026.443624
GGGCACC66700.026.21943994
CGCAGAG346800.026.162338
TACCTGG50700.025.1222762
CTAACGC24400.025.0432643
GTACATG304350.024.8116321
CTAGTAC85550.024.6696933
TAGGCAT72400.024.5968325
TACATGG306000.024.3140432
Produced by FastQC (version 0.11.5)