##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211186_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8202956 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07706465815494 33.0 31.0 34.0 30.0 34.0 2 32.23016958764621 33.0 31.0 34.0 30.0 34.0 3 32.30268966455507 34.0 31.0 34.0 30.0 34.0 4 35.93498075571782 37.0 35.0 37.0 35.0 37.0 5 35.81531694184389 37.0 35.0 37.0 35.0 37.0 6 35.842139589679626 37.0 35.0 37.0 35.0 37.0 7 35.77879766757252 37.0 35.0 37.0 33.0 37.0 8 35.76515258645786 37.0 35.0 37.0 33.0 37.0 9 37.35538249869925 39.0 37.0 39.0 34.0 39.0 10-11 37.33315179308532 39.0 37.0 39.0 34.0 39.0 12-13 37.23193517068725 39.0 37.0 39.0 33.5 39.0 14-15 38.438745739950335 40.0 38.0 41.0 34.0 41.0 16-17 38.3916784388457 40.0 38.0 41.0 33.5 41.0 18-19 38.40963940316149 40.0 38.0 41.0 34.0 41.0 20-21 38.37099723563067 40.0 38.0 41.0 34.0 41.0 22-23 38.240624903998025 40.0 38.0 41.0 34.0 41.0 24-25 38.172871157665604 40.0 37.5 41.0 33.0 41.0 26-27 38.033205712672356 40.0 37.0 41.0 33.0 41.0 28-29 38.00934382678635 40.0 37.0 41.0 33.0 41.0 30-31 38.02298464114644 40.0 37.0 41.0 33.0 41.0 32-33 37.89493391162893 40.0 37.0 41.0 33.0 41.0 34-35 37.749285684331355 40.0 37.0 41.0 33.0 41.0 36-37 37.60056728330616 40.0 37.0 41.0 32.5 41.0 38-39 37.506429511507804 40.0 36.0 41.0 32.0 41.0 40-41 37.408823173012266 40.0 36.0 41.0 32.0 41.0 42-43 37.26308857441147 39.0 36.0 41.0 31.5 41.0 44-45 37.120680886256125 39.0 36.0 41.0 31.0 41.0 46-47 36.885778065858204 39.0 35.5 41.0 31.0 41.0 48-49 36.65451533569119 39.0 35.0 41.0 30.0 41.0 50-51 36.45185820818739 39.0 35.0 40.0 30.0 41.0 52-53 36.239249119463764 39.0 35.0 40.0 29.5 41.0 54-55 36.0026057679695 38.0 35.0 40.0 29.0 41.0 56-57 35.73923692873642 38.0 34.5 40.0 28.5 41.0 58-59 35.45986050638331 38.0 34.0 40.0 28.0 41.0 60-61 35.198763835865996 37.0 34.0 40.0 28.0 41.0 62-63 34.8949820040483 37.0 34.0 40.0 27.0 41.0 64-65 34.516047324891176 36.0 33.0 39.0 26.0 41.0 66-67 34.210651562680575 36.0 33.0 39.0 26.0 40.0 68-69 33.88505521668043 35.0 33.0 38.5 26.0 40.0 70-71 33.56859161502268 35.0 32.5 38.0 26.0 40.0 72-73 33.231660635507495 35.0 32.0 37.0 25.5 39.5 74-75 32.89902249871875 35.0 32.0 37.0 25.0 39.0 76-77 31.92980347572265 34.0 30.5 36.0 24.5 38.0 78-79 32.437341611975 34.5 31.5 36.0 25.0 38.0 80-81 32.36999229302217 35.0 32.0 35.5 25.0 37.5 82-83 32.12136106788821 34.5 31.5 35.0 25.0 37.0 84-85 31.866740538410788 34.0 31.0 35.0 24.5 36.5 86-87 31.624541080069186 34.0 31.0 35.0 24.0 36.0 88-89 31.40197795770208 34.0 31.0 35.0 24.0 36.0 90-91 31.181012978735957 34.0 31.0 35.0 23.0 35.0 92-93 30.996521436906406 34.0 30.5 35.0 23.0 35.0 94-95 30.801204041567452 34.0 30.0 35.0 20.5 35.0 96-97 30.61970715190963 34.0 30.0 35.0 20.0 35.0 98-99 30.437701177477972 34.0 29.5 35.0 20.0 35.0 100 28.634921118679657 32.0 25.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4365300542767727 1101 2 -0.4240130355031262 1101 3 -0.49745839427060545 1101 4 -0.28043341272732647 1101 5 -0.3305212469047518 1101 6 -0.3593641937396086 1101 7 -0.3521613625456723 1101 8 -0.3941426376655528 1101 9 -0.5284292342417132 1101 10-11 -0.5393401895325098 1101 12-13 -0.5892232582385901 1101 14-15 -0.7640658285038029 1101 16-17 -0.7404253817950561 1101 18-19 -0.8989558646271405 1101 20-21 -0.8576897647655528 1101 22-23 -0.880350325996929 1101 24-25 -0.8358809466464479 1101 26-27 -0.9319633557484508 1101 28-29 -1.0029274791909373 1101 30-31 -0.7873653778647594 1101 32-33 -0.7701780364240989 1101 34-35 -0.832715539561498 1101 36-37 -0.8663618569989566 1101 38-39 -0.8807909303201313 1101 40-41 -0.9583067091923141 1101 42-43 -0.8826913372516643 1101 44-45 -0.9212974629797515 1101 46-47 -0.9012838311128561 1101 48-49 -0.8014989563503434 1101 50-51 -0.9394491319082974 1101 52-53 -0.9972667739195415 1101 54-55 -0.9244130819960148 1101 56-57 -0.89537138715297 1101 58-59 -0.8798200675271062 1101 60-61 -0.9367373527565732 1101 62-63 -1.0080863987208986 1101 64-65 -1.0079196116820057 1101 66-67 -1.0084375778201746 1101 68-69 -1.1026936682852622 1101 70-71 -1.1242673714348754 1101 72-73 -1.2437495185105547 1101 74-75 -1.2271584183116104 1101 76-77 -1.0622130515820736 1101 78-79 -0.9934481080353414 1101 80-81 -0.9576704238360065 1101 82-83 -0.8847624651295547 1101 84-85 -0.7846132274824882 1101 86-87 -1.049710110141465 1101 88-89 -1.0652506258420047 1101 90-91 -1.1054543391562213 1101 92-93 -1.157266947239851 1101 94-95 -1.2180954288114059 1101 96-97 -1.214479759104922 1101 98-99 -1.3115390396938231 1101 100 -1.0954544542423967 1102 1 -0.427301035385959 1102 2 -0.3966063109708813 1102 3 -0.3705435077960857 1102 4 -0.29632667702706783 1102 5 -0.31364226565259656 1102 6 -0.36407370797915917 1102 7 -0.38739288965797414 1102 8 -0.3846444948089385 1102 9 -0.5881638385455119 1102 10-11 -0.5578342705394235 1102 12-13 -0.560418441731926 1102 14-15 -0.6473747502872769 1102 16-17 -0.7212279787181117 1102 18-19 -0.7066079298902608 1102 20-21 -0.785336490264541 1102 22-23 -0.7037595967197205 1102 24-25 -0.78020063473938 1102 26-27 -0.7939352905527954 1102 28-29 -0.855973508680556 1102 30-31 -0.8048447448752682 1102 32-33 -0.7684657139709827 1102 34-35 -0.8192123363054193 1102 36-37 -0.9149707600936239 1102 38-39 -0.9403632625291038 1102 40-41 -0.8333965160586772 1102 42-43 -0.8776439622699996 1102 44-45 -0.8947623291682021 1102 46-47 -0.9107128994875779 1102 48-49 -0.963943262767522 1102 50-51 -1.0236616589014673 1102 52-53 -1.1518385152350206 1102 54-55 -1.0268265341710503 1102 56-57 -1.058941321031675 1102 58-59 -1.0587754842935766 1102 60-61 -1.0943386599582894 1102 62-63 -1.092611860133509 1102 64-65 -1.077839169847742 1102 66-67 -1.128036620532555 1102 68-69 -1.1812968325755975 1102 70-71 -1.243113540628734 1102 72-73 -1.1455809159512143 1102 74-75 -1.2493961025815743 1102 76-77 -1.0420886923236488 1102 78-79 -0.8903227268615765 1102 80-81 -0.8391506881576163 1102 82-83 -0.8625411185079273 1102 84-85 -1.005275383789936 1102 86-87 -0.9541714764953184 1102 88-89 -1.0097677804275165 1102 90-91 -1.0798338215817083 1102 92-93 -1.0325244659990318 1102 94-95 -1.1897008972030179 1102 96-97 -1.3295691453909306 1102 98-99 -1.317598390518551 1102 100 -1.2241598191938827 1103 1 -0.37896194383782245 1103 2 -0.3825082401805666 1103 3 -0.4171252220988926 1103 4 -0.26222608026662186 1103 5 -0.2628744205876288 1103 6 -0.3737251573376099 1103 7 -0.4595695779449471 1103 8 -0.39598518669209426 1103 9 -0.5913969141301862 1103 10-11 -0.5627667673113379 1103 12-13 -0.5836791783513462 1103 14-15 -0.790505233262742 1103 16-17 -0.8142709251957925 1103 18-19 -0.7806903130766045 1103 20-21 -0.8279125892692747 1103 22-23 -0.7776161797801038 1103 24-25 -0.8364088710638313 1103 26-27 -0.7942321335886788 1103 28-29 -0.8917621015988644 1103 30-31 -0.8590145482318121 1103 32-33 -0.8043049924741439 1103 34-35 -0.8436348945481384 1103 36-37 -0.8167229856893456 1103 38-39 -0.8979685098996839 1103 40-41 -0.8813872480748444 1103 42-43 -0.7132438137030661 1103 44-45 -0.7883676779099602 1103 46-47 -0.7923387933403347 1103 48-49 -0.8506616038033954 1103 50-51 -0.9265482860500001 1103 52-53 -0.947234934672359 1103 54-55 -0.9578164485705685 1103 56-57 -0.9305237831545909 1103 58-59 -0.9007964099619201 1103 60-61 -1.0506395844301224 1103 62-63 -0.9824137127331838 1103 64-65 -0.9079323648509714 1103 66-67 -1.0428422537783106 1103 68-69 -1.0309924697931123 1103 70-71 -1.1335128184006962 1103 72-73 -0.9536468620516629 1103 74-75 -0.8759553074992397 1103 76-77 -0.8806308004119039 1103 78-79 -0.7552942515338223 1103 80-81 -0.7284880613813343 1103 82-83 -0.8006265993313235 1103 84-85 -0.8285339184946068 1103 86-87 -0.8544617137869466 1103 88-89 -0.848391940999452 1103 90-91 -0.8304137976182311 1103 92-93 -0.9893156280948538 1103 94-95 -1.0840702688180954 1103 96-97 -1.1203413361216157 1103 98-99 -1.1666143423522932 1103 100 -1.2620427238601124 1104 1 -0.4158854532152958 1104 2 -0.361637164166428 1104 3 -0.4193759460828659 1104 4 -0.3045080515948939 1104 5 -0.34123276559694915 1104 6 -0.41470628182072744 1104 7 -0.4140060180720013 1104 8 -0.4143148919341968 1104 9 -0.5364334020507115 1104 10-11 -0.5904369552965747 1104 12-13 -0.645605702058873 1104 14-15 -0.8341387321300076 1104 16-17 -0.7321688185785789 1104 18-19 -0.7945180619095495 1104 20-21 -0.8617746469889767 1104 22-23 -0.784801598121895 1104 24-25 -0.8286257255995295 1104 26-27 -0.8703971903125591 1104 28-29 -0.9923701246559489 1104 30-31 -0.8906318743104151 1104 32-33 -0.8817980794894993 1104 34-35 -0.8557194889972664 1104 36-37 -0.8211074558609184 1104 38-39 -0.832591903861001 1104 40-41 -0.8946929185744352 1104 42-43 -0.802903045506504 1104 44-45 -0.9006502194187362 1104 46-47 -1.023946586146181 1104 48-49 -1.0374081211626773 1104 50-51 -0.9380869417459579 1104 52-53 -1.0111344463509013 1104 54-55 -1.014314109377814 1104 56-57 -1.0112283846202104 1104 58-59 -1.0026958373844792 1104 60-61 -1.0383503968683314 1104 62-63 -1.117267194502297 1104 64-65 -1.039683465444611 1104 66-67 -1.0250559087660776 1104 68-69 -0.9616384007743974 1104 70-71 -1.1175975007375172 1104 72-73 -1.0885909189912724 1104 74-75 -1.0886315640935393 1104 76-77 -0.9978790330339287 1104 78-79 -0.8640831300359864 1104 80-81 -0.8840507169552687 1104 82-83 -0.9096823368361981 1104 84-85 -0.8551342916904581 1104 86-87 -0.9191267563471079 1104 88-89 -0.9857625881071108 1104 90-91 -0.9138394856663048 1104 92-93 -1.035287883910513 1104 94-95 -1.0854073057824998 1104 96-97 -0.988364578422182 1104 98-99 -0.9781837405830593 1104 100 -0.9476120000649928 1105 1 -0.4128316464212034 1105 2 -0.37995270502307577 1105 3 -0.4394889184507349 1105 4 -0.28297925669461677 1105 5 -0.31617128275190964 1105 6 -0.4481618676347381 1105 7 -0.46846538077925004 1105 8 -0.5213274783419166 1105 9 -0.584691870573188 1105 10-11 -0.5349219647738792 1105 12-13 -0.6118221777712165 1105 14-15 -0.8329893202760346 1105 16-17 -0.7918933567335458 1105 18-19 -0.7673712327126481 1105 20-21 -0.8379620417381517 1105 22-23 -0.824710086764064 1105 24-25 -0.8422451781639708 1105 26-27 -0.9184432063428716 1105 28-29 -0.9161710248820327 1105 30-31 -0.8148466936988328 1105 32-33 -0.8267200405971664 1105 34-35 -0.8622786899917827 1105 36-37 -0.8376168089128697 1105 38-39 -0.8999162754486107 1105 40-41 -0.9709620147559335 1105 42-43 -0.9633401517654718 1105 44-45 -0.9782281387436029 1105 46-47 -0.7760896664987484 1105 48-49 -0.9483569042176825 1105 50-51 -0.9889598201427887 1105 52-53 -0.9873451612492161 1105 54-55 -0.9576314752114428 1105 56-57 -0.9136401231598015 1105 58-59 -1.1017498340480927 1105 60-61 -0.9884904436237107 1105 62-63 -0.9443457353981373 1105 64-65 -1.034325584762506 1105 66-67 -1.0211189389758317 1105 68-69 -0.9899797358376503 1105 70-71 -1.0497911194672795 1105 72-73 -1.0756443525191344 1105 74-75 -1.1221188487687321 1105 76-77 -1.1020323749713228 1105 78-79 -0.9966611985954259 1105 80-81 -0.8395503704793441 1105 82-83 -0.8993119725923719 1105 84-85 -0.8975126843945773 1105 86-87 -0.936256170049429 1105 88-89 -0.8963085996424027 1105 90-91 -0.9446689565812036 1105 92-93 -0.9281890650931501 1105 94-95 -0.8843365027890293 1105 96-97 -0.9034690947637252 1105 98-99 -1.088251931791742 1105 100 -1.1007986615869605 1106 1 -0.44455838615069965 1106 2 -0.4077802458450108 1106 3 -0.4978929034803521 1106 4 -0.3365454537381254 1106 5 -0.3513773506113935 1106 6 -0.4673230369075867 1106 7 -0.46310779266580226 1106 8 -0.4762715208723449 1106 9 -0.5705856921056807 1106 10-11 -0.5720127835751114 1106 12-13 -0.6697967039007224 1106 14-15 -0.7778104167280091 1106 16-17 -0.7734288574769508 1106 18-19 -0.7543056490821485 1106 20-21 -0.7796499945021225 1106 22-23 -0.7785378764060695 1106 24-25 -0.6745548129271768 1106 26-27 -0.8066044836238717 1106 28-29 -0.8509409160485504 1106 30-31 -0.8645452654611745 1106 32-33 -0.8929758080840671 1106 34-35 -0.8305225315642417 1106 36-37 -0.8640216084530579 1106 38-39 -0.7792357420942437 1106 40-41 -0.8208475418208749 1106 42-43 -0.8422107913290162 1106 44-45 -0.8849010881460586 1106 46-47 -0.8542109493563856 1106 48-49 -0.966078433033779 1106 50-51 -1.0024699209986707 1106 52-53 -0.9143850103239615 1106 54-55 -0.8865916010104016 1106 56-57 -0.9890651980457434 1106 58-59 -0.8497151541840822 1106 60-61 -0.9176247085724114 1106 62-63 -1.0154354259591543 1106 64-65 -1.067921301707166 1106 66-67 -1.0579745216100065 1106 68-69 -0.9910306603717132 1106 70-71 -0.9577500289095155 1106 72-73 -1.006787813341056 1106 74-75 -1.0156478955453352 1106 76-77 -1.0763730672250453 1106 78-79 -0.9443654408728861 1106 80-81 -0.7445469688333262 1106 82-83 -0.7498974151527129 1106 84-85 -0.7584462063379434 1106 86-87 -0.7728622172146196 1106 88-89 -0.8764095200847173 1106 90-91 -0.8519362997848638 1106 92-93 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2310 24-25 1.0593581965498302 2310 26-27 1.0755059836058436 2310 28-29 1.098823155647544 2310 30-31 0.9120081005210636 2310 32-33 1.048113123621178 2310 34-35 1.0706169945039292 2310 36-37 1.0060099616293101 2310 38-39 1.1186066494645104 2310 40-41 1.1662721618248426 2310 42-43 1.2381159273534692 2310 44-45 1.2391432910999782 2310 46-47 0.8753600486724338 2310 48-49 1.1209217507360947 2310 50-51 1.1675062403110061 2310 52-53 1.282437805644598 2310 54-55 1.285559733140893 2310 56-57 1.3287329637275889 2310 58-59 1.2963220837337488 2310 60-61 1.3182709529165209 2310 62-63 0.7701037316802584 2310 64-65 0.9498195135838188 2310 66-67 1.2344510894387355 2310 68-69 1.3816299511027879 2310 70-71 1.3846436481285949 2310 72-73 1.4096105997650312 2310 74-75 1.4006445060908987 2310 76-77 1.4009974010520132 2310 78-79 1.2812963772599346 2310 80-81 1.2165894027268749 2310 82-83 1.1728300091061001 2310 84-85 1.2327247898017788 2310 86-87 1.2700939929086665 2310 88-89 1.2444233589170572 2310 90-91 1.26712761004649 2310 92-93 1.3675147473332032 2310 94-95 1.3614454844391055 2310 96-97 1.4113774528592664 2310 98-99 1.471773405378027 2310 100 1.6014194328143319 2311 1 0.42034204538786213 2311 2 0.33936953096400657 2311 3 0.46082533587109964 2311 4 0.31455923015703746 2311 5 0.4385659010841749 2311 6 0.5187222858573932 2311 7 0.5537358778295953 2311 8 0.5309948070731423 2311 9 0.698438462478336 2311 10-11 0.7137257710157812 2311 12-13 0.7620736085555748 2311 14-15 0.9011546813478049 2311 16-17 0.7952383978485997 2311 18-19 0.8994230761884339 2311 20-21 0.8306395265296729 2311 22-23 0.9621657463522908 2311 24-25 1.0204358517939696 2311 26-27 0.45822710334860517 2311 28-29 0.7608639954546135 2311 30-31 0.9530780503600909 2311 32-33 1.0661702243670348 2311 34-35 1.103148262914793 2311 36-37 1.1226219018665589 2311 38-39 1.171109636915027 2311 40-41 1.1887994414661804 2311 42-43 1.1855212731522542 2311 44-45 1.1757765835894247 2311 46-47 0.8133073688090775 2311 48-49 1.1043753055501568 2311 50-51 1.2176674261260914 2311 52-53 1.1413994701834085 2311 54-55 1.1823210896241108 2311 56-57 1.2530128929049553 2311 58-59 1.259687314583175 2311 60-61 1.2717517108621408 2311 62-63 1.2597711077390983 2311 64-65 1.328412662839817 2311 66-67 1.3486706267450472 2311 68-69 1.3783183043151297 2311 70-71 1.3582797262879538 2311 72-73 1.3686867323083476 2311 74-75 1.4181151024665937 2311 76-77 1.3416310399099451 2311 78-79 1.0598664575894077 2311 80-81 1.0015947030926853 2311 82-83 1.0919808160902633 2311 84-85 1.169527284904035 2311 86-87 1.151458815021865 2311 88-89 1.2293970939783527 2311 90-91 1.302009870189206 2311 92-93 1.3652534594188097 2311 94-95 1.3832372998462965 2311 96-97 1.4261676527724099 2311 98-99 1.4499991002273838 2311 100 1.5316187990188865 2312 1 0.30410833523817615 2312 2 0.4222294803168083 2312 3 0.5052741453958944 2312 4 0.24241987563824807 2312 5 0.4305882760378452 2312 6 0.47700191718361395 2312 7 0.37359009250133823 2312 8 0.4865713063692141 2312 9 0.6722611871957795 2312 10-11 0.7062779468506406 2312 12-13 0.7572403409809851 2312 14-15 0.9701016524893973 2312 16-17 0.956963757217558 2312 18-19 0.9315147262422627 2312 20-21 0.9743810235156758 2312 22-23 1.0335698247476088 2312 24-25 1.0434499211076727 2312 26-27 1.050074150875247 2312 28-29 1.058866564414032 2312 30-31 1.0645445794934005 2312 32-33 0.9288154229957399 2312 34-35 1.0485898237064006 2312 36-37 1.1282984726097922 2312 38-39 0.9620404497056967 2312 40-41 1.0663066230206866 2312 42-43 1.1573157713514703 2312 44-45 0.9096575868384775 2312 46-47 0.9472539772162705 2312 48-49 1.0974352171907569 2312 50-51 0.9510008395116571 2312 52-53 1.1400083652483275 2312 54-55 1.0090517511312669 2312 56-57 1.036182004337796 2312 58-59 1.164931127309977 2312 60-61 1.229260906472831 2312 62-63 1.255788032896227 2312 64-65 1.2089138811623386 2312 66-67 1.2160693685326152 2312 68-69 1.281858379164909 2312 70-71 1.1549421013581238 2312 72-73 1.1801802937399728 2312 74-75 1.270575087253917 2312 76-77 1.24154117892569 2312 78-79 1.1441848372528511 2312 80-81 1.105393816821028 2312 82-83 1.0912137979892265 2312 84-85 1.1323557665577795 2312 86-87 1.15335906301571 2312 88-89 1.1647185766401513 2312 90-91 1.2721295525494973 2312 92-93 1.3285265732618257 2312 94-95 1.3662883878474013 2312 96-97 0.9986693341952773 2312 98-99 0.8115536451591403 2312 100 1.0000785639487333 2313 1 0.0936043299899012 2313 2 0.27483238244782626 2313 3 0.34061836718492344 2313 4 0.23598305828195265 2313 5 0.37963807177715836 2313 6 0.3326664158096122 2313 7 0.28743801567950555 2313 8 0.4458239195806257 2313 9 0.6042995892694876 2313 10-11 0.4307220902978699 2313 12-13 0.4713528700411729 2313 14-15 0.522712371055583 2313 16-17 0.7854033443928969 2313 18-19 0.7390720045021624 2313 20-21 0.7035799206247475 2313 22-23 0.6545879096897593 2313 24-25 0.5780914617393691 2313 26-27 0.759418893568693 2313 28-29 0.5809685814952772 2313 30-31 0.8796742312113892 2313 32-33 0.9587039685572449 2313 34-35 1.0299564613015875 2313 36-37 1.0428768966963915 2313 38-39 1.0120918595558663 2313 40-41 1.02737961767604 2313 42-43 1.0919225513736635 2313 44-45 0.9590109261229713 2313 46-47 0.9615223132465189 2313 48-49 1.026764314900305 2313 50-51 1.0194790489725065 2313 52-53 1.0368064501409506 2313 54-55 0.913431172455148 2313 56-57 0.659302637020204 2313 58-59 0.746544385335639 2313 60-61 0.6345881661937725 2313 62-63 1.0224796524376885 2313 64-65 1.0930695728622837 2313 66-67 1.0569524899067702 2313 68-69 1.0841770307231613 2313 70-71 1.1030727593411882 2313 72-73 1.1450390633695378 2313 74-75 1.206464103735442 2313 76-77 1.1616084969640745 2313 78-79 1.0010178854977525 2313 80-81 0.8908345682902947 2313 82-83 0.9791861011164045 2313 84-85 0.9562118900860241 2313 86-87 1.0406860586397464 2313 88-89 1.0044768741032577 2313 90-91 0.9392474918983105 2313 92-93 1.0881241456487736 2313 94-95 1.2572252089599658 2313 96-97 1.261477850543546 2313 98-99 1.3059338344190898 2313 100 1.379395318045031 2314 1 -0.3531427573386914 2314 2 0.08378175867218118 2314 3 0.3256683723400968 2314 4 0.2608309807481888 2314 5 0.3609763540743032 2314 6 0.466597748936735 2314 7 0.4914796694582506 2314 8 0.513562861739608 2314 9 0.49954652194296045 2314 10-11 0.6182156463345763 2314 12-13 0.4757863167882661 2314 14-15 0.7727896986151208 2314 16-17 0.8253559168230495 2314 18-19 0.8954283298965322 2314 20-21 0.9252522579791176 2314 22-23 0.9577119430952621 2314 24-25 0.8511608503361359 2314 26-27 0.8009180171896801 2314 28-29 0.8687302064050684 2314 30-31 0.9180287007443297 2314 32-33 0.9157098454517723 2314 34-35 0.9658261385879072 2314 36-37 0.925442109604937 2314 38-39 0.6270006130356052 2314 40-41 0.6881178381111397 2314 42-43 0.9307203655813154 2314 44-45 0.9827762627556069 2314 46-47 0.9704407584470545 2314 48-49 1.0055765635857412 2314 50-51 1.0475747289395159 2314 52-53 1.048096273834112 2314 54-55 1.0037497620004672 2314 56-57 0.9732525287616198 2314 58-59 1.046678419772178 2314 60-61 1.131907477462974 2314 62-63 1.0459356788321585 2314 64-65 1.0648707894825549 2314 66-67 0.7777484482529928 2314 68-69 0.7570814538595627 2314 70-71 0.8532531903134597 2314 72-73 1.0361103078277267 2314 74-75 1.0910398331920916 2314 76-77 0.865959460980978 2314 78-79 0.7725922746100338 2314 80-81 0.7993302894986698 2314 82-83 0.8480901118391628 2314 84-85 0.9062067349153864 2314 86-87 0.9186116179757597 2314 88-89 0.9541624086944545 2314 90-91 1.0390515954141328 2314 92-93 1.008425207613925 2314 94-95 1.0259127025160026 2314 96-97 0.9700045027757334 2314 98-99 1.060650815551167 2314 100 1.1262764398101623 2315 1 0.4158175768709853 2315 2 0.44852180467771774 2315 3 0.4563801477320695 2315 4 0.3246369111018623 2315 5 0.3748311524038357 2315 6 0.44800405920779696 2315 7 0.40390536069945426 2315 8 0.2859291573036984 2315 9 0.49313326153865233 2315 10-11 0.540401561622609 2315 12-13 0.3968898046683549 2315 14-15 0.6071890781442377 2315 16-17 0.7225062227778309 2315 18-19 0.7806008646175755 2315 20-21 0.5396594992226653 2315 22-23 0.853829250444754 2315 24-25 0.8652382367032772 2315 26-27 0.8543113741994119 2315 28-29 0.8352909560194703 2315 30-31 0.8920746187078521 2315 32-33 0.8168391066502352 2315 34-35 0.833220891517719 2315 36-37 0.8502490980518402 2315 38-39 0.9256885539856796 2315 40-41 0.8596027832701978 2315 42-43 0.9326738330530091 2315 44-45 0.9791872880908485 2315 46-47 1.0080400129086229 2315 48-49 0.8940134746581876 2315 50-51 0.9459035474586912 2315 52-53 0.9832363693191581 2315 54-55 0.8088626393252127 2315 56-57 0.8604451172548622 2315 58-59 0.7930823159531855 2315 60-61 0.90445870328778 2315 62-63 0.9716664630841194 2315 64-65 1.0552789402160698 2315 66-67 1.0783338056799465 2315 68-69 1.1357016621411304 2315 70-71 0.9871297715360967 2315 72-73 1.0819743115746832 2315 74-75 0.46948241463539375 2315 76-77 0.49892332035147646 2315 78-79 0.7450478683757353 2315 80-81 0.7584899789413058 2315 82-83 0.7243279468880175 2315 84-85 0.7985806184343858 2315 86-87 0.8778420761560533 2315 88-89 0.8356587554306536 2315 90-91 0.9241671285864719 2315 92-93 0.8528204059732971 2315 94-95 0.9038427452978972 2315 96-97 1.1101524267058203 2315 98-99 1.110234400068368 2315 100 1.1281875917908657 2316 1 0.3017130513845885 2316 2 0.09994480737654499 2316 3 0.2255979578627958 2316 4 0.07096059226648066 2316 5 0.27596501348933344 2316 6 0.38185733541206446 2316 7 0.4360228699620734 2316 8 0.4500976366616314 2316 9 0.5865196729100361 2316 10-11 0.6562753969280166 2316 12-13 0.4148046477277063 2316 14-15 0.5423773156328338 2316 16-17 0.7944664537041106 2316 18-19 0.7712918051695326 2316 20-21 0.7692290188935544 2316 22-23 0.8332132759305608 2316 24-25 0.4710011782132071 2316 26-27 0.7578638689909667 2316 28-29 0.9831383344824545 2316 30-31 0.6036072555380088 2316 32-33 0.7353389773226198 2316 34-35 0.8423647025050158 2316 36-37 0.898417791395623 2316 38-39 0.8318173603938988 2316 40-41 0.8011078595282584 2316 42-43 0.7612181337449542 2316 44-45 0.6522150074246085 2316 46-47 0.8209867970928428 2316 48-49 0.9202647813634144 2316 50-51 0.9314870838932308 2316 52-53 0.9457026811563409 2316 54-55 0.8068111144750532 2316 56-57 0.8920605356948741 2316 58-59 0.8058552005168735 2316 60-61 0.8869955644849128 2316 62-63 0.9626427442678036 2316 64-65 0.985718432327019 2316 66-67 0.9915975414693818 2316 68-69 0.9651076862006533 2316 70-71 0.9297161956486235 2316 72-73 0.9748440847302149 2316 74-75 0.9619528428414128 2316 76-77 0.7844054993706742 2316 78-79 0.4698543968412281 2316 80-81 0.4691066891496334 2316 82-83 0.4478664239107104 2316 84-85 0.5834841686831425 2316 86-87 0.640613421479248 2316 88-89 0.6354520385747016 2316 90-91 0.4704288600334898 2316 92-93 0.2946681360113743 2316 94-95 0.36322955523832334 2316 96-97 0.6055796887657863 2316 98-99 0.8116692423537053 2316 100 0.47980217024535676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 8.0 12 33.0 13 193.0 14 722.0 15 2070.0 16 5000.0 17 10224.0 18 17197.0 19 25421.0 20 36309.0 21 50329.0 22 61760.0 23 64716.0 24 66543.0 25 72596.0 26 86146.0 27 106136.0 28 131289.0 29 161990.0 30 200390.0 31 249683.0 32 317295.0 33 416272.0 34 583530.0 35 813123.0 36 1252373.0 37 1804249.0 38 1454285.0 39 213052.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.60315430920333 20.745298117905588 14.012437368304676 23.6391102045864 2 15.30854243275229 20.146700774696342 40.871912515439554 23.672844277111814 3 18.887800934199817 25.55000904551969 29.556345297963322 26.00584472231717 4 12.607555806455586 16.48241246477206 38.45309643036982 32.45693529840254 5 13.237630434687203 37.83716504148017 34.72232448887937 14.202880034953253 6 31.009955435577126 37.463519248427026 17.42368238961662 14.102842926379221 7 27.529516491297194 32.04599559763946 22.247317768703446 18.177170142359895 8 26.01164750853229 36.17897011760151 19.486975183092532 18.322407190773667 9 25.88285052013712 15.917827065076759 21.051910323103883 37.14741209168224 10-11 25.636591947537703 26.387086938985565 27.283529967592635 20.692791145884097 12-13 25.575039726537568 23.815393355199436 28.120116470223234 22.48945044803976 14-15 22.774994256632027 25.29733396180723 25.736926378647556 26.190745402913183 16-17 21.94085409673489 28.121849069875598 26.802589560931544 23.13470727245797 18-19 21.58427401049085 27.559242058594485 29.089531871193753 21.76695205972091 20-21 22.683246464231487 26.74952501872801 28.834608991515864 21.732619525524644 22-23 22.72983056351881 26.586239155738493 28.56695805755876 22.116972223183932 24-25 22.310428767561156 26.526361511396086 29.14052044482772 22.022689276215036 26-27 22.28370782116527 26.835809394331733 28.52959762045551 22.350885164047483 28-29 21.833969731332846 27.075683127191745 28.86926610928914 22.221081032186273 30-31 22.454217759325417 26.757728877068455 28.80795133921039 21.98010202439574 32-33 22.56511495504015 26.986194885174204 28.27569482034013 22.172995339445517 34-35 22.578597969380727 26.863819448915528 28.723486888185224 21.834095693518524 36-37 22.439820449096757 26.67096723842321 28.493864978306092 22.395347334173938 38-39 22.40823669449446 26.81415932440727 28.8056046746592 21.97199930643907 40-41 22.29309189531127 26.28045580009047 29.11063050582234 22.315821798775925 42-43 22.24432266849316 26.471874178458815 28.638777152345714 22.64502600070231 44-45 22.341722106543198 26.973287847976117 28.501405166401316 22.18358487907937 46-47 22.34502667568618 26.841686789060898 28.04275108529245 22.770535449960473 48-49 22.80625260470844 26.819544092437443 28.05380330253715 22.32040000031697 50-51 22.558752116088122 26.835496513369534 27.924359108002943 22.681392262539404 52-53 22.257055622933635 27.182874540930733 27.747367047841347 22.812702788294278 54-55 22.2133965836976 27.140248668833955 27.732797162584983 22.913557584883456 56-57 22.780217359475767 26.87945215532685 27.501591203803432 22.838739281393956 58-59 22.163671899335927 27.114703024815995 27.992949740260325 22.728675335587756 60-61 22.304526412832278 27.477090705518247 27.57440781335987 22.643975068289606 62-63 22.471053188670542 27.086170636010316 27.36272097740705 23.080055197912092 64-65 22.017856849958108 27.17607759907215 27.542502350443986 23.263563200525752 66-67 22.239410657587563 26.98807034895079 27.521943259825534 23.250575733636122 68-69 22.05318080511319 26.992904871311787 27.682980284613873 23.27093403896115 70-71 22.0335647450767 26.728105146912995 27.733964395653825 23.504365712356478 72-73 22.335988947260198 26.892329210265252 27.74315869018984 23.028523152284713 74-75 22.46661601650724 27.145196852698277 27.41079311808235 22.977394012712132 76-77 22.305126699404763 27.050719699300068 27.217139739848417 23.42701386144675 78-79 22.178239695908502 27.08661160031613 27.281204407551467 23.4539442962239 80-81 21.909418472486433 27.425858574702083 27.458012019841632 23.20671093296985 82-83 22.448803380664113 26.797185153946877 28.08037967713638 22.673631788252628 84-85 22.58542246877602 27.067623411083197 27.32147443014334 23.02547968999744 86-87 21.850338582705497 27.46849208764075 27.569233486519053 23.111935843134702 88-89 22.260145504877187 26.81767948791613 27.505507639085348 23.416667368121338 90-91 22.153985289606677 26.867933940442484 27.53856736795437 23.43951340199647 92-93 22.316722727517195 26.99224766306073 27.416195014440127 23.274834594981954 94-95 21.660965235136427 26.930429123784005 27.82283346363871 23.585772177440862 96-97 21.837954807287886 26.88938940464362 27.559240001706765 23.713415786361725 98-99 21.4884145204279 27.219806199118796 27.41547389180017 23.87630538865314 100 21.808674812984794 26.893861652587255 27.114049551301306 24.183413983126652 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1285.0 1 898.5 2 853.0 3 1238.5 4 991.0 5 797.0 6 1227.0 7 2350.0 8 3744.5 9 4495.5 10 4167.5 11 3739.0 12 4112.5 13 4742.5 14 5434.0 15 6507.5 16 7650.0 17 8688.5 18 9765.5 19 10862.5 20 11961.5 21 13359.0 22 15547.0 23 18542.0 24 22184.0 25 26780.5 26 33151.0 27 41126.5 28 50736.5 29 64518.5 30 82671.5 31 100781.0 32 123665.5 33 148150.5 34 167427.5 35 187021.5 36 206519.0 37 234228.0 38 250849.0 39 249248.0 40 244229.0 41 237656.5 42 240333.0 43 251080.0 44 266795.0 45 296603.5 46 323365.0 47 340663.5 48 355067.0 49 353988.5 50 342069.5 51 340483.5 52 336433.5 53 318289.0 54 301427.0 55 270852.5 56 237272.5 57 200941.0 58 167934.0 59 153042.5 60 126571.0 61 94517.5 62 72705.0 63 58065.5 64 42812.0 65 26654.0 66 18262.5 67 13516.0 68 10082.0 69 7573.5 70 5928.5 71 4473.5 72 3300.0 73 2527.0 74 1932.5 75 1410.0 76 967.0 77 617.0 78 415.0 79 258.5 80 144.0 81 101.0 82 70.0 83 49.5 84 36.0 85 19.0 86 16.0 87 12.0 88 5.5 89 4.0 90 2.5 91 3.0 92 2.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044288912435956014 2 0.0 3 0.0 4 0.03831545603804287 5 0.0 6 0.0 7 0.00102402109678511 8 0.0 9 0.010825365880299736 10-11 4.4496154800781573E-4 12-13 1.2190727342679883E-5 14-15 2.0114700115421807E-4 16-17 2.560052741962775E-4 18-19 0.0 20-21 1.2190727342679883E-5 22-23 0.0 24-25 6.095363671339941E-4 26-27 5.059151847212151E-4 28-29 2.1943309216823788E-4 30-31 0.0025844341966481353 32-33 0.015232313814678513 34-35 0.025698053238369194 36-37 0.030428055447328985 38-39 0.02196769067150915 40-41 0.0012861217346527276 42-43 5.485827304205947E-5 44-45 0.011002131426768594 46-47 0.018042276467166225 48-49 0.003200065927453469 50-51 0.011105752609181372 52-53 0.010953368517397874 54-55 0.0102706877862078 56-57 5.729641851059546E-4 58-59 0.008594462776589318 60-61 0.004754383663645154 62-63 0.0013348846440234471 64-65 0.03546892120352712 66-67 0.060191716254481914 68-69 0.05932617461315165 70-71 0.052346983209467415 72-73 0.04537388716945452 74-75 0.03554816093125454 76-77 0.03195799172883532 78-79 0.014799542994013377 80-81 0.001249549552624688 82-83 0.03836421894741359 84-85 0.051804495842718165 86-87 0.057753570785945944 88-89 0.06954809948998873 90-91 0.04865319282463541 92-93 0.039437002953569424 94-95 0.013025792165653454 96-97 0.0037303625668600442 98-99 0.022022548944551208 100 0.03275648436978085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8202956.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.88399972028018 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2422308585698 47.31532424263559 2 14.815401501298266 18.633034077269574 3 4.903792531064215 9.251102644552624 4 2.0323048350200765 5.1119782670770615 5 0.9953485877458661 3.1295750156696154 6 0.5568151511731816 2.100885828637327 7 0.3540525286226427 1.5584967377609527 8 0.23334220685337914 1.1738793016397961 9 0.16281834287959798 0.9214801765287409 >10 0.6629871404119848 6.8863098123793485 >50 0.02812715596159582 1.2068806244022516 >100 0.011852705369260495 1.3040608528225113 >500 5.508809622319718E-4 0.22179829398277892 >1k 3.0045925451248047E-4 0.3481706527527876 >5k 0.0 0.0 >10k+ 7.511481337182264E-5 0.8370234718890349 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22273 0.271524070103509 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18865 0.229978071319656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12023 0.14656911484104024 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.2190727342679883E-5 0.0 0.0 0.0 3.657218202803965E-5 7 1.2190727342679883E-5 0.0 0.0 1.2190727342679883E-5 3.657218202803965E-5 8 1.2190727342679883E-5 0.0 0.0 1.2190727342679883E-5 3.657218202803965E-5 9 1.2190727342679883E-5 0.0 0.0 2.4381454685359766E-5 3.657218202803965E-5 10-11 1.2190727342679883E-5 0.0 0.0 3.0476818356699708E-5 3.657218202803965E-5 12-13 1.2190727342679883E-5 0.0 0.0 4.876290937071953E-5 4.2667545699379594E-5 14-15 1.8286091014019825E-5 0.0 0.0 4.876290937071953E-5 7.31443640560793E-5 16-17 2.4381454685359766E-5 0.0 0.0 6.0953636713399416E-5 8.533509139875918E-5 18-19 2.4381454685359766E-5 0.0 0.0 8.533509139875918E-5 1.1581190975545888E-4 20-21 2.4381454685359766E-5 0.0 0.0 1.0971654608411894E-4 1.2190727342679883E-4 22-23 2.4381454685359766E-5 0.0 0.0 2.07242364825558E-4 1.2190727342679883E-4 24-25 4.876290937071953E-5 1.2190727342679883E-5 0.0 5.485827304205947E-4 1.2800263709813878E-4 26-27 5.485827304205947E-5 1.2190727342679883E-5 0.0 0.0012373588252820081 1.3409800076947872E-4 28-29 6.0953636713399416E-5 1.2190727342679883E-5 0.0 0.003291496382523568 1.3409800076947872E-4 30-31 6.0953636713399416E-5 1.2190727342679883E-5 0.0 0.011782337976700107 1.3409800076947872E-4 32-33 6.0953636713399416E-5 1.2190727342679883E-5 0.0 0.03448147228877005 1.462887281121586E-4 34-35 8.533509139875918E-5 1.2190727342679883E-5 0.0 0.06868865321232981 1.8286091014019825E-4 36-37 1.0971654608411894E-4 1.8286091014019825E-5 0.0 0.12613745581470875 1.8286091014019825E-4 38-39 1.0971654608411894E-4 2.4381454685359766E-5 0.0 0.20972317783979336 1.8286091014019825E-4 40-41 1.0971654608411894E-4 4.2667545699379594E-5 0.0 0.29669182670247163 1.8286091014019825E-4 42-43 1.0971654608411894E-4 6.0953636713399416E-5 0.0 0.3834166610182963 1.889562738115382E-4 44-45 1.0971654608411894E-4 6.0953636713399416E-5 0.0 0.4871536553408308 2.0114700115421807E-4 46-47 1.5238409178349854E-4 7.31443640560793E-5 0.0 0.6114015484174241 2.1943309216823788E-4 48-49 1.5847945545483847E-4 7.31443640560793E-5 0.0 0.7444389559080897 2.3162381951091776E-4 50-51 1.5847945545483847E-4 7.31443640560793E-5 0.0 0.8837728740712494 2.3162381951091776E-4 52-53 1.8286091014019825E-4 7.31443640560793E-5 0.0 1.0155851134639757 2.4381454685359764E-4 54-55 1.889562738115382E-4 7.31443640560793E-5 0.0 1.1610692535715175 2.560052741962775E-4 56-57 2.1333772849689795E-4 7.31443640560793E-5 0.0 1.3377433695853056 2.560052741962775E-4 58-59 2.3162381951091776E-4 7.31443640560793E-5 0.0 1.5310273516034951 2.560052741962775E-4 60-61 2.3162381951091776E-4 7.31443640560793E-5 0.0 1.725591359992666 2.560052741962775E-4 62-63 2.3162381951091776E-4 7.31443640560793E-5 0.0 1.9235321998557593 2.6819600153895744E-4 64-65 2.3162381951091776E-4 7.31443640560793E-5 0.0 2.128226702666697 2.6819600153895744E-4 66-67 2.3771918318225773E-4 7.31443640560793E-5 0.0 2.357381899890722 2.803867288816373E-4 68-69 2.4381454685359766E-4 7.31443640560793E-5 0.0 2.610455304161085 3.0476818356699707E-4 70-71 2.4381454685359766E-4 7.31443640560793E-5 0.0 2.8807968737123546 3.1695891090967695E-4 72-73 2.4381454685359766E-4 7.923972772741924E-5 0.0 3.1517296935397434 3.9010327496575626E-4 74-75 2.560052741962775E-4 8.533509139875918E-5 0.0 3.4215531571789484 3.9010327496575626E-4 76-77 2.560052741962775E-4 9.752581874143907E-5 0.0 3.7209635160788377 3.9010327496575626E-4 78-79 2.6819600153895744E-4 9.752581874143907E-5 0.0 4.050356968878049 3.9010327496575626E-4 80-81 2.6819600153895744E-4 9.752581874143907E-5 0.0 4.397262401505019 3.9619863863709617E-4 82-83 2.6819600153895744E-4 9.752581874143907E-5 0.0 4.751079976535288 4.266754569937959E-4 84-85 2.6819600153895744E-4 9.752581874143907E-5 0.0 5.09640061460771 4.266754569937959E-4 86-87 2.925774562243172E-4 9.752581874143907E-5 0.0 5.461281274701461 4.266754569937959E-4 88 2.925774562243172E-4 9.752581874143907E-5 0.0 5.7533040528316866 4.3886618433647577E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13095 0.0 48.0434 1 GTATCAA 23480 0.0 40.19133 1 CAACGCA 27640 0.0 32.75836 5 ATCAACG 27650 0.0 32.7075 3 GTACTAG 5360 0.0 31.49291 1 TATCAAC 30285 0.0 31.116053 2 AACGCAG 31295 0.0 29.698183 6 TCAACGC 31210 0.0 29.157413 4 GTGGTAT 5215 0.0 28.942352 1 CTGTGCG 7395 0.0 27.448511 9 ACGCAGA 33870 0.0 26.843819 7 TAGTACT 7910 0.0 26.44362 4 GGGCACC 6670 0.0 26.219439 94 CGCAGAG 34680 0.0 26.16233 8 TACCTGG 5070 0.0 25.122276 2 CTAACGC 2440 0.0 25.043264 3 GTACATG 30435 0.0 24.811632 1 CTAGTAC 8555 0.0 24.669693 3 TAGGCAT 7240 0.0 24.596832 5 TACATGG 30600 0.0 24.314043 2 >>END_MODULE