FastQCFastQC Report
Mon 6 Jun 2016
ERR1211185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7459611
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268650.360139422819769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206360.2766364090567189No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132040.1770065490010136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136050.048.252681
GTATCAA248000.041.461551
TACCTGG50450.035.4456022
GTACTAG58050.034.77771
CAACGCA291250.033.7629475
ATCAACG293050.033.6036833
TATCAAC315850.032.1521872
AACGCAG325600.030.980816
GCATCGT59100.030.3011268
TCAACGC331500.030.0036284
TAGGCAT71800.029.0671945
ACCTGGG60300.028.7642883
GTACATG347850.028.6671641
ACGCAGA352350.028.042187
TACATGG352300.027.9440292
GTGGTAT50450.027.8904691
CGCAGAG357250.027.6423578
ACATGGG352600.026.9419233
GTACCTG68750.026.9008261
TCTAACG26750.026.3880582
Produced by FastQC (version 0.11.5)