FastQCFastQC Report
Mon 6 Jun 2016
ERR1211185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7459611
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240150.32193367723866567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196930.2639949992030416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126620.1697407545782213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140200.046.6145941
GTATCAA243550.039.2082021
TACCTGG50550.034.494562
GTACTAG55100.033.961481
ATCAACG288150.031.7108233
CAACGCA289300.031.5419125
TATCAAC314350.030.0823922
AACGCAG323700.028.7124846
GTGGTAT49250.028.2579461
TCAACGC328000.028.245074
TAGGCAT71250.027.5003625
GTACATG348900.027.450181
ACCTGGG63100.026.8910473
TACATGG352850.026.8533362
CTGTGCG76300.026.7269579
GGGCACC68800.026.16845794
ACATGGG352000.025.985443
GTACCTG67850.025.8472371
ACGCAGA357750.025.5987877
CTAGGCA81600.025.2874034
Produced by FastQC (version 0.11.5)