##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211185_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7459611 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07897502966308 33.0 31.0 34.0 30.0 34.0 2 32.22036175881021 33.0 31.0 34.0 30.0 34.0 3 32.2957632777366 34.0 31.0 34.0 30.0 34.0 4 35.92911882402447 37.0 35.0 37.0 35.0 37.0 5 35.813057812263935 37.0 35.0 37.0 35.0 37.0 6 35.839151398109095 37.0 35.0 37.0 35.0 37.0 7 35.7783211483816 37.0 35.0 37.0 33.0 37.0 8 35.76404332611982 37.0 35.0 37.0 33.0 37.0 9 37.35207117368453 39.0 37.0 39.0 34.0 39.0 10-11 37.32669625802204 39.0 37.0 39.0 34.0 39.0 12-13 37.21773890086226 39.0 37.0 39.0 33.0 39.0 14-15 38.405848709805376 40.0 38.0 41.0 33.5 41.0 16-17 38.358434776290615 40.0 38.0 41.0 33.0 41.0 18-19 38.386318669968176 40.0 38.0 41.0 33.5 41.0 20-21 38.34845329066087 40.0 38.0 41.0 34.0 41.0 22-23 38.21286445097472 40.0 38.0 41.0 33.5 41.0 24-25 38.141626218847065 40.0 37.0 41.0 33.0 41.0 26-27 37.99575514058307 40.0 37.0 41.0 33.0 41.0 28-29 37.970313666490114 40.0 37.0 41.0 33.0 41.0 30-31 37.979715832367134 40.0 37.0 41.0 33.0 41.0 32-33 37.83662834429302 40.0 37.0 41.0 33.0 41.0 34-35 37.68883129428599 40.0 37.0 41.0 33.0 41.0 36-37 37.537737423573425 40.0 36.0 41.0 32.0 41.0 38-39 37.43935963953081 40.0 36.0 41.0 32.0 41.0 40-41 37.33038686601755 39.5 36.0 41.0 32.0 41.0 42-43 37.176724161621834 39.0 36.0 41.0 31.0 41.0 44-45 37.01635406993743 39.0 35.5 41.0 31.0 41.0 46-47 36.75915265554732 39.0 35.0 41.0 30.5 41.0 48-49 36.50048769299096 39.0 35.0 40.5 30.0 41.0 50-51 36.284707339296915 39.0 35.0 40.0 29.5 41.0 52-53 36.06663403761939 38.5 35.0 40.0 29.0 41.0 54-55 35.81469140951184 38.0 35.0 40.0 28.5 41.0 56-57 35.54104349409104 38.0 34.0 40.0 28.0 41.0 58-59 35.255358623928245 37.0 34.0 40.0 28.0 41.0 60-61 34.98271042551683 37.0 34.0 40.0 27.0 41.0 62-63 34.66879305100494 36.0 34.0 39.5 26.5 41.0 64-65 34.288679664395374 36.0 33.0 39.0 26.0 40.5 66-67 33.98495792877135 35.0 33.0 39.0 26.0 40.0 68-69 33.65981852136794 35.0 33.0 38.0 26.0 40.0 70-71 33.35044099484544 35.0 32.0 37.5 25.5 40.0 72-73 33.02210483897887 35.0 32.0 37.0 25.0 39.5 74-75 32.69455223603483 35.0 32.0 36.5 25.0 39.0 76-77 31.73073823822717 34.0 30.5 35.5 23.5 38.0 78-79 32.24705215861792 34.5 31.5 36.0 25.0 38.0 80-81 32.193274287358946 35.0 32.0 35.0 25.0 37.0 82-83 31.95319394000572 34.0 31.0 35.0 24.0 37.0 84-85 31.71178450189963 34.0 31.0 35.0 24.0 36.5 86-87 31.484113849904507 34.0 31.0 35.0 24.0 36.0 88-89 31.27508056385246 34.0 31.0 35.0 23.0 36.0 90-91 31.064819264704283 34.0 31.0 35.0 22.5 35.0 92-93 30.881118532856473 34.0 30.0 35.0 20.5 35.0 94-95 30.69153163616709 34.0 30.0 35.0 20.0 35.0 96-97 30.518313823602867 34.0 30.0 35.0 20.0 35.0 98-99 30.339806660159624 34.0 29.0 35.0 19.5 35.0 100 28.51678016454209 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3670926922089244 1101 2 -0.3571381578476682 1101 3 -0.38902524525823523 1101 4 -0.20831610960144786 1101 5 -0.300698624292842 1101 6 -0.3222107241285812 1101 7 -0.3189368646012909 1101 8 -0.37515792801376335 1101 9 -0.6091077417363522 1101 10-11 -0.5065517528654411 1101 12-13 -0.5131241449639674 1101 14-15 -0.7264481476348124 1101 16-17 -0.7082701829016784 1101 18-19 -0.6694631842265366 1101 20-21 -0.6632959834528052 1101 22-23 -0.7088645893266659 1101 24-25 -0.7001795585289798 1101 26-27 -0.881281489653361 1101 28-29 -0.9616689302114949 1101 30-31 -0.7117650054140867 1101 32-33 -0.6507350860813688 1101 34-35 -0.6698042716544919 1101 36-37 -0.6972674835865362 1101 38-39 -0.7536032921740272 1101 40-41 -0.7935214855828363 1101 42-43 -0.7614628039522771 1101 44-45 -0.8145916013356498 1101 46-47 -0.7888852521571934 1101 48-49 -0.739128453807723 1101 50-51 -0.7796786406860576 1101 52-53 -0.8088860192101706 1101 54-55 -0.7194539165844986 1101 56-57 -0.8000716689512615 1101 58-59 -0.8498875445551022 1101 60-61 -0.8307611843520348 1101 62-63 -0.9681462015695104 1101 64-65 -0.9087610898907457 1101 66-67 -0.9351096266055734 1101 68-69 -1.0563373036955497 1101 70-71 -1.009703359851109 1101 72-73 -1.1013386547953345 1101 74-75 -1.061185211283572 1101 76-77 -1.0888686758551032 1101 78-79 -1.0020840362300447 1101 80-81 -0.8686674345691543 1101 82-83 -0.8689171400788247 1101 84-85 -0.8286372112021461 1101 86-87 -0.9805854496433248 1101 88-89 -1.133070176414737 1101 90-91 -1.0770871250797889 1101 92-93 -1.0598339817130231 1101 94-95 -1.1139235927377094 1101 96-97 -1.152942258673857 1101 98-99 -1.1960441602895528 1101 100 -1.094904886386331 1102 1 -0.3394232292340291 1102 2 -0.327760906837522 1102 3 -0.38008822148126953 1102 4 -0.2186476595531488 1102 5 -0.2793474530530773 1102 6 -0.347339159198917 1102 7 -0.403642190491766 1102 8 -0.37106899614173017 1102 9 -0.49940146446689937 1102 10-11 -0.5171985187434913 1102 12-13 -0.5019796817942108 1102 14-15 -0.624400607592996 1102 16-17 -0.7434782462637131 1102 18-19 -0.69988136282889 1102 20-21 -0.8146920477720911 1102 22-23 -0.730321149122382 1102 24-25 -0.7833833212868697 1102 26-27 -0.8845341577665025 1102 28-29 -0.8161309696662897 1102 30-31 -0.7811479880595584 1102 32-33 -0.7189156632477847 1102 34-35 -0.7659243549601413 1102 36-37 -0.8228275554236646 1102 38-39 -0.877174365161622 1102 40-41 -0.8124010801292414 1102 42-43 -0.7211862649927951 1102 44-45 -0.8371920974675078 1102 46-47 -0.8648606306657456 1102 48-49 -0.800258281133587 1102 50-51 -0.8781175778864636 1102 52-53 -0.881797313332946 1102 54-55 -0.8970833910759382 1102 56-57 -0.9891382361577925 1102 58-59 -0.9979902547090447 1102 60-61 -1.0442655062325699 1102 62-63 -0.9160449007009461 1102 64-65 -0.9399303393484715 1102 66-67 -1.0941673865426722 1102 68-69 -0.9938142820163662 1102 70-71 -1.066513872035344 1102 72-73 -1.079266389598338 1102 74-75 -1.1925085109946707 1102 76-77 -1.011367706845867 1102 78-79 -0.8628088785003598 1102 80-81 -0.8637802722642043 1102 82-83 -0.812277188366739 1102 84-85 -0.8497737845309139 1102 86-87 -0.7662234566123338 1102 88-89 -0.9934549675645101 1102 90-91 -0.9429163802922886 1102 92-93 -0.972557083358268 1102 94-95 -1.0637800584151336 1102 96-97 -1.1907598077438841 1102 98-99 -1.1574390681075357 1102 100 -1.075069568067807 1103 1 -0.38032896793759363 1103 2 -0.36774786737969833 1103 3 -0.43431758564664946 1103 4 -0.2575569602902803 1103 5 -0.23697247456430404 1103 6 -0.37763664025806776 1103 7 -0.4169734213098124 1103 8 -0.4230098724006268 1103 9 -0.529987352564369 1103 10-11 -0.5672116076254738 1103 12-13 -0.6067999075765727 1103 14-15 -0.7378758459378361 1103 16-17 -0.7668072717414915 1103 18-19 -0.7757201537331895 1103 20-21 -0.7958107070513236 1103 22-23 -0.7581213997455052 1103 24-25 -0.9093357962892128 1103 26-27 -0.7958972885589048 1103 28-29 -0.9213630031199713 1103 30-31 -0.9141146786017913 1103 32-33 -0.7669154524281652 1103 34-35 -0.8924340767570627 1103 36-37 -0.8943042058509505 1103 38-39 -0.8049359861139962 1103 40-41 -0.8477967062867364 1103 42-43 -0.8365465665074652 1103 44-45 -0.9660420960252623 1103 46-47 -0.9576229476040794 1103 48-49 -0.9412893015708619 1103 50-51 -0.9809914076681991 1103 52-53 -0.9807712037828438 1103 54-55 -0.9237156230187722 1103 56-57 -0.9698883019537163 1103 58-59 -0.8969193736837013 1103 60-61 -0.9756352402636779 1103 62-63 -0.8810163980839576 1103 64-65 -0.8328182597036502 1103 66-67 -1.074090588209323 1103 68-69 -1.0119779817490553 1103 70-71 -1.0731543066850762 1103 72-73 -1.0590513953381482 1103 74-75 -0.9613213928575846 1103 76-77 -0.8969458785957727 1103 78-79 -0.7285304983466503 1103 80-81 -0.7392361728936834 1103 82-83 -0.7749951036589238 1103 84-85 -0.8989348711313774 1103 86-87 -0.8827822195357378 1103 88-89 -0.8606792843723774 1103 90-91 -1.050816146895201 1103 92-93 -1.0453439534542213 1103 94-95 -1.1404010506214135 1103 96-97 -1.124639820616327 1103 98-99 -1.2426468377053794 1103 100 -1.2997634269152059 1104 1 -0.42144573485147063 1104 2 -0.3538887458737925 1104 3 -0.4693288944063525 1104 4 -0.2880665784561458 1104 5 -0.34185251258004 1104 6 -0.3919242157166707 1104 7 -0.3991061615266176 1104 8 -0.4184811606498613 1104 9 -0.5435018582727054 1104 10-11 -0.519193581739124 1104 12-13 -0.6214669958245125 1104 14-15 -0.8180497315648267 1104 16-17 -0.7501563695507301 1104 18-19 -0.8055872617198077 1104 20-21 -0.8465735787693802 1104 22-23 -0.7837125910703193 1104 24-25 -0.7908483219827858 1104 26-27 -0.8296923251010639 1104 28-29 -0.9840584262738545 1104 30-31 -0.9828099511543442 1104 32-33 -0.9853363001599789 1104 34-35 -0.9453914249920743 1104 36-37 -0.9055631492989562 1104 38-39 -0.8857271536362887 1104 40-41 -0.9634636712533151 1104 42-43 -0.863733030589735 1104 44-45 -0.9804036701813885 1104 46-47 -1.1111762852334763 1104 48-49 -1.0819307095601118 1104 50-51 -0.9292058726697761 1104 52-53 -0.9455091489318548 1104 54-55 -0.9866330422652112 1104 56-57 -0.9364522668803374 1104 58-59 -0.92402573476285 1104 60-61 -0.9921008575703354 1104 62-63 -0.9786168595865377 1104 64-65 -1.0067432231887992 1104 66-67 -1.0904289115350068 1104 68-69 -1.1032201046335928 1104 70-71 -1.1768011042212194 1104 72-73 -1.1105042456817458 1104 74-75 -1.145161562842432 1104 76-77 -0.9881413014081524 1104 78-79 -0.8923930476549273 1104 80-81 -0.8350193228587983 1104 82-83 -0.8957312035147744 1104 84-85 -0.830383626040863 1104 86-87 -0.9160255207646948 1104 88-89 -1.0111840549512259 1104 90-91 -1.0022556275289283 1104 92-93 -1.1192721313044913 1104 94-95 -1.0956753584785304 1104 96-97 -1.04973834752359 1104 98-99 -1.2033197422039876 1104 100 -1.3259183273834374 1105 1 -0.410257830708467 1105 2 -0.4052839811558542 1105 3 -0.45498018505173476 1105 4 -0.28638218877487986 1105 5 -0.28920993474546464 1105 6 -0.3878350621212334 1105 7 -0.43657566869821096 1105 8 -0.3955663275717285 1105 9 -0.5506644076540113 1105 10-11 -0.48559118812744373 1105 12-13 -0.5787973813503129 1105 14-15 -0.8340489217717533 1105 16-17 -0.729904007486823 1105 18-19 -0.6854296936710682 1105 20-21 -0.8282278975987794 1105 22-23 -0.7996849053905919 1105 24-25 -0.8212497221411397 1105 26-27 -0.8335212598313433 1105 28-29 -0.802606756653347 1105 30-31 -0.7546067975830155 1105 32-33 -0.7857499376379238 1105 34-35 -0.7573689778496586 1105 36-37 -0.6975181665999912 1105 38-39 -0.8156679965444695 1105 40-41 -0.8855349029023643 1105 42-43 -0.9118561894659081 1105 44-45 -0.9117091550726215 1105 46-47 -0.8632660738031319 1105 48-49 -0.8885813109771448 1105 50-51 -1.0075940428589476 1105 52-53 -1.041053930689607 1105 54-55 -0.917593661807139 1105 56-57 -0.9827782578219129 1105 58-59 -1.0381859612637854 1105 60-61 -0.96693045942542 1105 62-63 -1.0044815096072313 1105 64-65 -1.0094034336144304 1105 66-67 -0.9956376593095868 1105 68-69 -0.9770116944402503 1105 70-71 -0.9670767553712096 1105 72-73 -1.0732351773657562 1105 74-75 -1.0127651681295973 1105 76-77 -1.037462572240731 1105 78-79 -0.9304309672704036 1105 80-81 -0.9019666541586098 1105 82-83 -0.7609940948980132 1105 84-85 -0.8386842561924936 1105 86-87 -0.8302341740969013 1105 88-89 -0.8295872053074973 1105 90-91 -0.7865193688934795 1105 92-93 -0.8265178703461586 1105 94-95 -0.913739922898273 1105 96-97 -0.9098614139668122 1105 98-99 -1.0212109948312929 1105 100 -1.033717659454858 1106 1 -0.5362134873355515 1106 2 -0.4973104636402823 1106 3 -0.5171516879105944 1106 4 -0.37347187701414697 1106 5 -0.47589622132750975 1106 6 -0.5210589655714273 1106 7 -0.5659939222365082 1106 8 -0.5220373810915149 1106 9 -0.6200115405709496 1106 10-11 -0.7083580551934006 1106 12-13 -0.7640328605757389 1106 14-15 -0.8705154683195744 1106 16-17 -0.9033346217478666 1106 18-19 -0.8796508853929339 1106 20-21 -0.9535225987377416 1106 22-23 -0.8981700711148761 1106 24-25 -0.9508407597054358 1106 26-27 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0.8423825962096458 2310 20-21 0.955584563096707 2310 22-23 1.004006350343687 2310 24-25 1.0368531623320862 2310 26-27 1.080300852502731 2310 28-29 1.0074907830097217 2310 30-31 0.8707802549799979 2310 32-33 1.0415635348530472 2310 34-35 1.0615479316438936 2310 36-37 0.9763934230610474 2310 38-39 1.0360222336969187 2310 40-41 1.1917705122883078 2310 42-43 1.2562021743945238 2310 44-45 1.252403145967456 2310 46-47 0.8883104377161857 2310 48-49 1.1027531884489719 2310 50-51 1.2096265178808139 2310 52-53 1.246949157069288 2310 54-55 1.3167346373249629 2310 56-57 1.3172750261121209 2310 58-59 1.3103707848921857 2310 60-61 1.3175170172497062 2310 62-63 0.7320216059493561 2310 64-65 0.8821601600381896 2310 66-67 1.26035548654648 2310 68-69 1.400467964905154 2310 70-71 1.4507709604445935 2310 72-73 1.4528435843561098 2310 74-75 1.3877030768413405 2310 76-77 1.3317311105538288 2310 78-79 1.2017412889469128 2310 80-81 1.1467300573751373 2310 82-83 1.1506598482843167 2310 84-85 1.1868805504866877 2310 86-87 1.2508933473848707 2310 88-89 1.2512417931982718 2310 90-91 1.3106378493800008 2310 92-93 1.3279000884093222 2310 94-95 1.4098925971311687 2310 96-97 1.4699903397650296 2310 98-99 1.4882170788575522 2310 100 1.6699126276952363 2311 1 0.4655818716883289 2311 2 0.36808034194895356 2311 3 0.4521594241856235 2311 4 0.35757132023053373 2311 5 0.46451921528559126 2311 6 0.49875243486851417 2311 7 0.5671590265373538 2311 8 0.5631563583352133 2311 9 0.7098568257262201 2311 10-11 0.739168229214151 2311 12-13 0.7734017279314003 2311 14-15 0.9356007084423226 2311 16-17 0.7966739596728871 2311 18-19 0.8785526583194994 2311 20-21 0.8161676434230642 2311 22-23 0.980746780858567 2311 24-25 1.0252267360892162 2311 26-27 0.4268938224911736 2311 28-29 0.7548813686872862 2311 30-31 0.9916872234190564 2311 32-33 1.1076975714562138 2311 34-35 1.1347954105301312 2311 36-37 1.154246832386029 2311 38-39 1.1618709545563348 2311 40-41 1.1445991246905791 2311 42-43 1.1383999989823224 2311 44-45 1.1464459791530217 2311 46-47 0.878344229820577 2311 48-49 1.1278907190113188 2311 50-51 1.2291659892081768 2311 52-53 1.1191490079519113 2311 54-55 1.1895345401523727 2311 56-57 1.2225212545169626 2311 58-59 1.19062739665857 2311 60-61 1.322333945431545 2311 62-63 1.320170608027155 2311 64-65 1.375854996680765 2311 66-67 1.3940521815588838 2311 68-69 1.4089066151912988 2311 70-71 1.430622341235761 2311 72-73 1.4793175179204994 2311 74-75 1.464139451184188 2311 76-77 1.3965536530276559 2311 78-79 1.1005964323772375 2311 80-81 1.066984161461754 2311 82-83 1.1410886839148695 2311 84-85 1.2049509580742104 2311 86-87 1.2614444939766578 2311 88-89 1.282216396223614 2311 90-91 1.3341795328391584 2311 92-93 1.3892474233278733 2311 94-95 1.4552130315866876 2311 96-97 1.479213899416461 2311 98-99 1.5406524949084925 2311 100 1.7027100564278754 2312 1 0.28915920343004586 2312 2 0.3730270952685899 2312 3 0.49900018637816146 2312 4 0.16232913201518784 2312 5 0.38909763071687564 2312 6 0.4624073902402941 2312 7 0.3513256156695306 2312 8 0.4441727843601342 2312 9 0.6430232146400741 2312 10-11 0.6527592951098242 2312 12-13 0.7130960529446071 2312 14-15 0.923043371158208 2312 16-17 0.9060885840177448 2312 18-19 0.8802491802487822 2312 20-21 0.8871455126528573 2312 22-23 0.9466236268651471 2312 24-25 0.9273260628893993 2312 26-27 1.0469477944091352 2312 28-29 1.0158946883218007 2312 30-31 1.0089261012280701 2312 32-33 0.8759424065514239 2312 34-35 1.0308168704951157 2312 36-37 1.081338321345747 2312 38-39 0.8619522906117112 2312 40-41 0.9635512404960949 2312 42-43 1.0761835536950954 2312 44-45 0.8415363092094381 2312 46-47 0.9401783760002047 2312 48-49 1.0579355007185 2312 50-51 0.9369488408360667 2312 52-53 1.049621257882599 2312 54-55 0.8733217851904769 2312 56-57 0.9957163768168087 2312 58-59 1.060192940836778 2312 60-61 1.152871672449372 2312 62-63 1.2083714815929767 2312 64-65 1.1619740506818985 2312 66-67 1.1319533526672743 2312 68-69 1.2350223905910696 2312 70-71 1.1240043279901286 2312 72-73 1.135354469487929 2312 74-75 1.234707545350517 2312 76-77 1.1513298238723273 2312 78-79 1.0274762508558055 2312 80-81 1.0195046495556213 2312 82-83 1.051555729540592 2312 84-85 1.1057767742539575 2312 86-87 1.1208569030503277 2312 88-89 1.1722336537136577 2312 90-91 1.2372307867248509 2312 92-93 1.2711173023592934 2312 94-95 1.2909849169892311 2312 96-97 0.9228184220297386 2312 98-99 0.7481188528507445 2312 100 0.9446581522018533 2313 1 0.10658687307377335 2313 2 0.2643575289698532 2313 3 0.34065603735759 2313 4 0.18954294442720965 2313 5 0.31426254589266733 2313 6 0.2851252747044981 2313 7 0.31141622414278913 2313 8 0.4025116616780906 2313 9 0.6107287541724915 2313 10-11 0.4046853515959654 2313 12-13 0.4826587168584098 2313 14-15 0.5539204695640905 2313 16-17 0.8005992756673024 2313 18-19 0.7433788053560875 2313 20-21 0.7413178107512053 2313 22-23 0.7071352226397636 2313 24-25 0.5856836335729909 2313 26-27 0.768305356327815 2313 28-29 0.5775427085518743 2313 30-31 0.8199433383815418 2313 32-33 0.9041764120723528 2313 34-35 0.9591368716219293 2313 36-37 0.9874251845881545 2313 38-39 1.0086243835257491 2313 40-41 1.0226662981998658 2313 42-43 1.0684709455902563 2313 44-45 0.9089814608244069 2313 46-47 0.9579036467806645 2313 48-49 1.0607563464340615 2313 50-51 1.0513580371554312 2313 52-53 1.1046112593177781 2313 54-55 0.9889990479836754 2313 56-57 0.7334842948039153 2313 58-59 0.8391918057238712 2313 60-61 0.7297925053952241 2313 62-63 1.0750287267285117 2313 64-65 1.2336752409831035 2313 66-67 1.1809520104882196 2313 68-69 1.2301417533825614 2313 70-71 1.2013128135214473 2313 72-73 1.1824586378851905 2313 74-75 1.2461834180804416 2313 76-77 1.1663558093984356 2313 78-79 1.0365011327180582 2313 80-81 0.9482328759215193 2313 82-83 1.0102426706414 2313 84-85 0.9971474346695679 2313 86-87 1.0406452046395351 2313 88-89 1.0247726744877461 2313 90-91 0.9625655114542617 2313 92-93 1.156883748658501 2313 94-95 1.257966037570256 2313 96-97 1.36345711125686 2313 98-99 1.2990984562735193 2313 100 1.3609769220059036 2314 1 -0.3638764786033555 2314 2 0.05580927967788796 2314 3 0.2975119882881927 2314 4 0.2246178539437338 2314 5 0.3452468980966046 2314 6 0.4424549514876972 2314 7 0.4622334736951359 2314 8 0.4891628364250309 2314 9 0.4786085215612488 2314 10-11 0.6257592616385139 2314 12-13 0.4972795091008493 2314 14-15 0.7646880948213735 2314 16-17 0.8285365800771771 2314 18-19 0.8470292049210428 2314 20-21 0.8944346696067598 2314 22-23 0.9097760848878664 2314 24-25 0.8356599371474189 2314 26-27 0.8255803982610814 2314 28-29 0.8437325252467716 2314 30-31 0.8888751049819348 2314 32-33 0.93238426593458 2314 34-35 0.9348310230657475 2314 36-37 0.9305810305952278 2314 38-39 0.6765335681772839 2314 40-41 0.7168310979642101 2314 42-43 0.8969215700038902 2314 44-45 0.9686058794379164 2314 46-47 0.9082778378791829 2314 48-49 0.9459021828561163 2314 50-51 0.9003393374648638 2314 52-53 0.8927253544449201 2314 54-55 0.8116473810152129 2314 56-57 0.8191774917982002 2314 58-59 0.9047774441419563 2314 60-61 0.9994639142281798 2314 62-63 0.9323649337631394 2314 64-65 0.9543149240990232 2314 66-67 0.7214903683720735 2314 68-69 0.6270609531372955 2314 70-71 0.7711693328244209 2314 72-73 0.9407711877075045 2314 74-75 0.9947704713985459 2314 76-77 0.8101444989101729 2314 78-79 0.7558586084727423 2314 80-81 0.7744907752183678 2314 82-83 0.8187655595084102 2314 84-85 0.8593397791296944 2314 86-87 0.9196468240539879 2314 88-89 0.9214147750053279 2314 90-91 1.081080394323358 2314 92-93 0.9246797764685581 2314 94-95 0.9121939364842575 2314 96-97 0.9911271404963706 2314 98-99 1.0934819956546633 2314 100 1.0825940613118803 2315 1 0.4083280253290411 2315 2 0.4657303597195508 2315 3 0.44490135345657933 2315 4 0.3228099701579126 2315 5 0.3783529476870484 2315 6 0.4471917934628067 2315 7 0.4051610074971279 2315 8 0.29146198545253554 2315 9 0.5316845551570069 2315 10-11 0.5928698685264209 2315 12-13 0.43543900536232627 2315 14-15 0.6436593100809063 2315 16-17 0.7998610797364094 2315 18-19 0.785699502291564 2315 20-21 0.5219261912876121 2315 22-23 0.8889993375652736 2315 24-25 0.9345873809360512 2315 26-27 0.8841910296306423 2315 28-29 0.8799018421145846 2315 30-31 0.9452670429498724 2315 32-33 0.903558722713818 2315 34-35 0.9040762943966811 2315 36-37 0.9260736052346061 2315 38-39 0.9563946112837911 2315 40-41 0.9910634620217778 2315 42-43 1.0340560885461585 2315 44-45 1.0028510802913573 2315 46-47 0.9628728222554344 2315 48-49 0.8436962434084023 2315 50-51 0.9268429068409318 2315 52-53 0.9405686812399239 2315 54-55 0.7769616216239044 2315 56-57 0.7801613353886054 2315 58-59 0.7947939606050056 2315 60-61 0.9106157106377353 2315 62-63 0.9561346130583885 2315 64-65 0.9473226724196806 2315 66-67 0.9939922378803985 2315 68-69 1.0331742391592158 2315 70-71 0.9131541806573225 2315 72-73 0.9694288461137148 2315 74-75 0.49127147391001813 2315 76-77 0.42022787502458314 2315 78-79 0.7232852141973964 2315 80-81 0.7460233009537873 2315 82-83 0.6794105485337063 2315 84-85 0.775527953055299 2315 86-87 0.8014494232640494 2315 88-89 0.7866309653430328 2315 90-91 0.8543886994904177 2315 92-93 0.9172528826516846 2315 94-95 0.9509952230818612 2315 96-97 1.0609584074789744 2315 98-99 1.1204765582540084 2315 100 1.0958209439512672 2316 1 0.29721961793845253 2316 2 0.10769679769087759 2316 3 0.26389044718619914 2316 4 0.0725993501174571 2316 5 0.30811173788823254 2316 6 0.396737012203225 2316 7 0.4758224085197611 2316 8 0.42738721630757226 2316 9 0.6594531994024138 2316 10-11 0.6519408137926703 2316 12-13 0.42622375325109374 2316 14-15 0.49057972261357463 2316 16-17 0.7960112974314697 2316 18-19 0.7867269690620233 2316 20-21 0.7948178184775188 2316 22-23 0.795557059127276 2316 24-25 0.49594930980078544 2316 26-27 0.7685269463943598 2316 28-29 0.9377968342287701 2316 30-31 0.5919414953145647 2316 32-33 0.7427926082481662 2316 34-35 0.8263010423515595 2316 36-37 0.9058247006387106 2316 38-39 0.8174141550512957 2316 40-41 0.8499783945621502 2316 42-43 0.8114905446714715 2316 44-45 0.6549281765006114 2316 46-47 0.8046606684680739 2316 48-49 0.9007889679062515 2316 50-51 0.9335411752541418 2316 52-53 0.948821711848943 2316 54-55 0.7923881037654539 2316 56-57 0.9613396392519107 2316 58-59 0.840000896614356 2316 60-61 0.892974468203569 2316 62-63 0.8635839076511331 2316 64-65 0.9359807894389505 2316 66-67 0.9036775496029534 2316 68-69 0.8836720299165464 2316 70-71 0.8896021453628364 2316 72-73 0.9366337481031053 2316 74-75 0.9732710773784632 2316 76-77 0.8247054078380707 2316 78-79 0.5475827135504403 2316 80-81 0.5395831721642992 2316 82-83 0.4821950797858001 2316 84-85 0.6201632746593582 2316 86-87 0.7331617129896593 2316 88-89 0.7105372854952847 2316 90-91 0.5557682516970885 2316 92-93 0.4497987086485793 2316 94-95 0.4792190713080444 2316 96-97 0.6253773670267151 2316 98-99 0.875487390405155 2316 100 0.42327392670697606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 29.0 13 192.0 14 729.0 15 2205.0 16 5088.0 17 10694.0 18 17811.0 19 25991.0 20 36886.0 21 50586.0 22 61528.0 23 63425.0 24 63103.0 25 68091.0 26 79954.0 27 97852.0 28 120945.0 29 149284.0 30 184541.0 31 229539.0 32 292312.0 33 385354.0 34 547025.0 35 751507.0 36 1157900.0 37 1636614.0 38 1243378.0 39 177031.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24221266734081 20.619928812259097 13.626001496712863 23.511857023687224 2 15.592864024679034 20.153892743200686 40.793186132628094 23.460057099492186 3 19.04053710039304 26.202934710670572 29.14794082426014 25.608587364676254 4 12.561231123756066 16.425966395028986 38.419633546609674 32.593168934605266 5 13.090468122265356 38.1843637691027 34.21199845407488 14.513169654557053 6 30.492193225625304 37.24199559467645 17.928079627744665 14.337731551953581 7 27.687749152394233 31.998376307603916 22.337192060476642 17.976682479525216 8 26.609524276802098 35.35409554197933 19.59504322678488 18.44133695443368 9 26.750890325255128 15.558205076399112 21.093753665976163 36.5971509323696 10-11 26.005190910263288 26.138174304230077 26.987960981170783 20.86867380433585 12-13 25.94939279667436 23.732742060241755 27.748186567394274 22.569678575689615 14-15 23.24217474902478 25.02864429344143 25.685321507142273 26.043859450391516 16-17 22.053904969716502 27.64438610939837 26.805285089040748 23.49642383184439 18-19 21.652965550080292 27.214984802826848 29.223903230342707 21.90814641675015 20-21 22.972995708019877 26.47077216699183 28.769685763083743 21.786546361904552 22-23 23.0219645501622 26.254646522452713 28.50582959352706 22.217559333858024 24-25 22.46670535075784 26.17101429244677 29.186194994228554 22.176085362566837 26-27 22.3379899913802 26.497834325571045 28.490248097410444 22.673927585638314 28-29 21.807418014672912 26.922546888256832 28.91979955485531 22.350235542214946 30-31 22.767577684990012 26.471108105820058 28.807759138374596 21.953555070815337 32-33 22.834494860995108 26.640228362378398 28.189327433484753 22.335949343141746 34-35 22.8194339128027 26.634371121869588 28.718536650448495 21.827658314879216 36-37 22.585774145543915 26.428296290783432 28.405834415326453 22.580095148346203 38-39 22.667000359213365 26.55981935013622 28.71019204573689 22.062988244913523 40-41 22.456499364737734 25.82612079132464 29.202525498465555 22.514854345472077 42-43 22.462828465739342 26.068233362459093 28.516673668823795 22.95226450297777 44-45 22.365533084723356 26.720728052281835 28.48038660348407 22.433352259510738 46-47 22.401982227129523 26.536991952900653 27.918229108530756 23.14279671143907 48-49 22.947862676392667 26.499400922662886 27.939948152579554 22.612788248364897 50-51 22.71261372050004 26.515932014555954 27.796908417939743 22.97454584700426 52-53 22.369115213898798 26.9024261308508 27.57172672232418 23.15673193292622 54-55 22.301117215038037 26.81149846032091 27.675310146276384 23.21207417836467 56-57 23.020339630608984 26.51908453229574 27.279121129554966 23.181454707540308 58-59 22.224783641446592 26.834151754816094 27.901624519999235 23.039440083738075 60-61 22.5176664725231 27.313928673722376 27.351284943747412 22.817119910007115 62-63 22.683724679770137 26.77979005230159 27.202947051115657 23.333538216812613 64-65 22.106240764533986 26.789821411784683 27.43171605368227 23.672221769999062 66-67 22.417385136487358 26.577839133940945 27.38552413645463 23.61925159311707 68-69 22.146525866571665 26.62190699821791 27.52891970247199 23.702647432738434 70-71 22.100685378294603 26.2897543184278 27.726949032160757 23.882611271116836 72-73 22.486832235444044 26.50780007234173 27.61185642647128 23.393511265742944 74-75 22.68611328020495 26.77521388874473 27.24444219153709 23.294230639513234 76-77 22.411058811334538 26.612918203493624 27.061168478608867 23.91485450656297 78-79 22.32447374906047 26.60618455633388 27.210272716522354 23.859068978083304 80-81 21.971720058676638 26.982419462894935 27.399242110173866 23.646618368254558 82-83 22.754163623868664 26.28881591612398 28.05988695844761 22.897133501559747 84-85 22.881244313992738 26.602982938256282 27.11217691776459 23.403595829986386 86-87 21.896824796429325 27.09744727174787 27.49026342799229 23.515464503830515 88-89 22.441605211765538 26.214223374334868 27.460024913750292 23.884146500149306 90-91 22.43445599954936 26.1588358999257 27.52312883748253 23.883579263042414 92-93 22.60462911934002 26.406066786768438 27.355852896138206 23.633451197753335 94-95 21.767184485258145 26.30606887751032 27.807906807937776 24.118839829293755 96-97 21.954257115838306 26.28010552131514 27.49428032966965 24.2713570331769 98-99 21.537823915342297 26.617747296807252 27.34266815849023 24.501760629360216 100 21.88545682246294 26.262811021847078 26.98466827755847 24.867063878131507 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1363.0 1 995.5 2 1169.0 3 1623.5 4 1181.5 5 930.0 6 1364.5 7 2577.5 8 4024.5 9 4679.5 10 4333.5 11 3844.0 12 4080.0 13 4778.5 14 5626.5 15 6641.5 16 7601.0 17 8505.5 18 9532.0 19 10415.5 20 11429.5 21 12605.5 22 13851.5 23 15718.0 24 18044.0 25 21015.0 26 25824.0 27 31401.5 28 37818.0 29 48814.0 30 64758.0 31 81111.5 32 102797.0 33 126547.0 34 146303.0 35 166818.0 36 186842.0 37 213780.0 38 225860.0 39 220439.0 40 212571.0 41 202159.5 42 200637.5 43 209429.0 44 226448.5 45 257700.0 46 286825.5 47 306170.0 48 320751.5 49 322505.5 50 316281.0 51 319921.0 52 320540.5 53 307950.0 54 294489.5 55 264444.0 56 233646.5 57 199131.0 58 166375.0 59 152623.5 60 126440.5 61 93245.5 62 71566.0 63 57440.0 64 41675.0 65 25012.5 66 16084.0 67 11340.0 68 8623.0 69 6755.5 70 5544.0 71 4443.5 72 3578.0 73 3245.0 74 2600.5 75 1800.5 76 1204.0 77 735.5 78 467.5 79 313.5 80 199.0 81 110.5 82 81.0 83 55.5 84 35.0 85 21.0 86 11.0 87 7.5 88 5.5 89 2.0 90 2.5 91 3.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043849471507294416 2 0.0 3 0.0 4 0.03510906936031919 5 0.0 6 0.0 7 9.383867335709598E-4 8 0.0 9 0.010992530307545528 10-11 4.42382317254881E-4 12-13 1.3405524765299423E-5 14-15 1.3405524765299425E-4 16-17 2.7481325768863817E-4 18-19 0.0 20-21 6.702762382649711E-6 22-23 0.0 24-25 5.563292777599261E-4 26-27 5.496265153772763E-4 28-29 1.8767734671419192E-4 30-31 0.0023392640715447495 32-33 0.014940457350926209 34-35 0.025819040697966693 36-37 0.030946653920693717 38-39 0.022092304813213448 40-41 0.0012534165655554961 42-43 6.702762382649713E-5 44-45 0.010804852960831337 46-47 0.01787626727452678 48-49 0.002996134785044421 50-51 0.010838366772744584 52-53 0.010456309316933551 54-55 0.010074251861122516 56-57 4.8259889155077926E-4 58-59 0.008559427562643681 60-61 0.004752258529298645 62-63 0.0011595778921984 64-65 0.035176096984145686 66-67 0.05918539183879695 68-69 0.05781802831273642 70-71 0.05096110239528576 72-73 0.04469401956750828 74-75 0.034586253894472514 76-77 0.030584704752030636 78-79 0.014705860667533469 80-81 0.001119361317902502 82-83 0.03747514448139454 84-85 0.05104823830626021 86-87 0.05650428688573707 88-89 0.06827433762966996 90-91 0.04760301844157826 92-93 0.03860120856167969 94-95 0.012735248527034454 96-97 0.003552464062804347 98-99 0.021777274981228913 100 0.03295077987310598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7459611.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.90865750445879 #Duplication Level Percentage of deduplicated Percentage of total 1 77.39847233074049 50.23830931884364 2 14.278715815559066 18.536245489512446 3 4.283434864401476 8.340960196682797 4 1.7137108039693545 4.449386705461502 5 0.7970228222722487 2.586684069705326 6 0.4254445492877603 1.656902072211484 7 0.2644131777167641 1.2013893074458124 8 0.16730065209766812 0.8687408581824124 9 0.11113618508114549 0.6492330516405792 >10 0.5087903755398224 5.668072045871508 >50 0.030616289879052412 1.3672378221066475 >100 0.019153987950195066 2.382840816508993 >500 0.001218019812571131 0.5356214180544598 >1k 4.664664757443131E-4 0.4838546698180819 >5k 2.5914804168234112E-5 0.08632512299797881 >10k+ 7.774441250470235E-5 0.9481970349563652 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24015 0.32193367723866567 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19693 0.2639949992030416 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12662 0.1697407545782213 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.3405524765299424E-5 0.0 6 0.0 0.0 0.0 1.3405524765299424E-5 0.0 7 0.0 0.0 0.0 1.3405524765299424E-5 0.0 8 0.0 0.0 0.0 1.3405524765299424E-5 1.3405524765299424E-5 9 0.0 0.0 0.0 9.383867335709597E-5 2.681104953059885E-5 10-11 0.0 0.0 0.0 1.0054143573974569E-4 2.681104953059885E-5 12-13 0.0 0.0 0.0 1.072441981223954E-4 2.681104953059885E-5 14-15 0.0 0.0 0.0 1.139469605050451E-4 4.0216574295898274E-5 16-17 0.0 0.0 0.0 1.2735248527034453E-4 6.702762382649711E-5 18-19 0.0 0.0 0.0 1.5416353480094338E-4 6.702762382649711E-5 20-21 0.0 0.0 0.0 2.0778563386214107E-4 6.702762382649711E-5 22-23 0.0 0.0 0.0 3.083270696018867E-4 7.373038620914683E-5 24-25 0.0 0.0 0.0 7.037900501782198E-4 9.383867335709597E-5 26-27 0.0 0.0 0.0 0.0014008773379737898 9.383867335709597E-5 28-29 0.0 0.0 0.0 0.0035189502508910986 9.383867335709597E-5 30-31 0.0 0.0 0.0 0.013465849626743272 9.383867335709597E-5 32-33 0.0 0.0 0.0 0.040095924573010575 1.139469605050451E-4 34-35 0.0 0.0 0.0 0.08012482152219466 1.3405524765299423E-4 36-37 0.0 0.0 0.0 0.14419652713794326 1.3405524765299423E-4 38-39 0.0 6.702762382649712E-6 0.0 0.2418356667660016 1.3405524765299423E-4 40-41 0.0 1.3405524765299424E-5 0.0 0.35212291901011994 1.3405524765299423E-4 42-43 0.0 1.3405524765299424E-5 0.0 0.45517118788097666 1.3405524765299423E-4 44-45 0.0 1.3405524765299424E-5 0.0 0.580593277585118 1.4075801003564394E-4 46-47 0.0 1.3405524765299424E-5 0.0 0.7256343527832751 1.4746077241829366E-4 48-49 0.0 1.3405524765299424E-5 0.0 0.8843892798163335 1.4746077241829366E-4 50-51 0.0 1.3405524765299424E-5 0.0 1.048466200181216 1.4746077241829366E-4 52-53 0.0 1.3405524765299424E-5 0.0 1.2101234233259617 1.742718219488925E-4 54-55 0.0 1.3405524765299424E-5 0.0 1.3862318021677003 2.0108287147949135E-4 56-57 0.0 1.3405524765299424E-5 0.0 1.5915575222354088 2.0108287147949135E-4 58-59 0.0 1.3405524765299424E-5 0.0 1.8181779183927955 2.0778563386214107E-4 60-61 0.0 1.3405524765299424E-5 0.0 2.0494098150694455 2.3459668339273991E-4 62-63 0.0 1.3405524765299424E-5 0.0 2.286808253138133 2.4129944577538963E-4 64-65 0.0 1.3405524765299424E-5 0.0 2.5283489983592977 2.4129944577538963E-4 66-67 0.0 1.3405524765299424E-5 0.0 2.799388600826504 2.5470497054068907E-4 68-69 0.0 1.3405524765299424E-5 0.0 3.097748662765391 2.6811049530598845E-4 70-71 0.0 1.3405524765299424E-5 0.0 3.4198163952517096 2.815160200712879E-4 72-73 0.0 1.3405524765299424E-5 0.0 3.738459016160494 3.351381191324856E-4 74-75 0.0 1.3405524765299424E-5 0.0 4.0483277211103905 3.351381191324856E-4 76-77 0.0 1.3405524765299424E-5 0.0 4.395142052313451 3.5524640628043473E-4 78-79 0.0 1.3405524765299424E-5 0.0 4.772567899318075 3.753546934283839E-4 80-81 0.0 1.3405524765299424E-5 0.0 5.1680710964687036 3.8205745581103355E-4 82-83 0.0 1.3405524765299424E-5 0.0 5.570766357655915 4.2227403010693186E-4 84-85 0.0 1.3405524765299424E-5 0.0 5.971651872999812 4.289767924895816E-4 86-87 0.0 1.3405524765299424E-5 0.0 6.38769233409088 4.289767924895816E-4 88 0.0 1.3405524765299424E-5 0.0 6.720484486389438 4.289767924895816E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14020 0.0 46.614594 1 GTATCAA 24355 0.0 39.208202 1 TACCTGG 5055 0.0 34.49456 2 GTACTAG 5510 0.0 33.96148 1 ATCAACG 28815 0.0 31.710823 3 CAACGCA 28930 0.0 31.541912 5 TATCAAC 31435 0.0 30.082392 2 AACGCAG 32370 0.0 28.712484 6 GTGGTAT 4925 0.0 28.257946 1 TCAACGC 32800 0.0 28.24507 4 TAGGCAT 7125 0.0 27.500362 5 GTACATG 34890 0.0 27.45018 1 ACCTGGG 6310 0.0 26.891047 3 TACATGG 35285 0.0 26.853336 2 CTGTGCG 7630 0.0 26.726957 9 GGGCACC 6880 0.0 26.168457 94 ACATGGG 35200 0.0 25.98544 3 GTACCTG 6785 0.0 25.847237 1 ACGCAGA 35775 0.0 25.598787 7 CTAGGCA 8160 0.0 25.287403 4 >>END_MODULE