FastQCFastQC Report
Mon 6 Jun 2016
ERR1211184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8390534
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285720.3405265981879103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229350.2733437466554572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136630.16283826512114724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165000.045.7776761
GTATCAA296700.040.5579341
GTACTAG89450.035.8285681
GGGCACC90600.035.5338494
CAACGCA327200.034.3803335
GCATCGT88000.034.0802848
ATCAACG336550.033.411813
TATCAAC371550.031.981132
TAGGCAT108950.031.7995515
TACCCCG6400.030.8496365
AACGCAG377300.030.8122376
CTGTGCG119050.030.7199559
CTAGGCA120300.029.306994
TAGTACT126050.029.2380714
GTGGTAT51050.029.1309071
CTAACGC46450.029.0458893
TCTAACG46550.028.7110042
TCAACGC399950.028.5494234
TACCTGG34600.028.426272
TAACGCC47550.028.373114
Produced by FastQC (version 0.11.5)