FastQCFastQC Report
Mon 6 Jun 2016
ERR1211184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8390534
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246720.2940456471542812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217090.2587320425612959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130180.15515103091173935No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC99690.11881246175749957No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166950.046.55511
GTATCAA286650.037.6617771
GTACTAG88250.035.32481
GGGCACC92150.034.4373594
GTGGTAT52700.032.476681
TAGGCAT104600.032.4315645
CAACGCA323750.031.8847245
ATCAACG327850.031.4412123
CTAGGCA119000.029.9009344
TAACGCC42350.029.7452224
CTAACGC42050.029.6220913
CTGTGCG120250.029.3822339
TATCAAC366350.029.3406372
TCTAACG43450.029.314272
TACCTGG33800.028.6450822
AACGCAG374600.028.4847056
CGCCTAC44200.028.3825477
TAGTACT127750.027.889724
ACTGTGC135400.027.3438458
GCATCGT107400.027.2980988
Produced by FastQC (version 0.11.5)