##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211184_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8390534 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16495636630517 33.0 31.0 34.0 30.0 34.0 2 32.31239644580428 34.0 31.0 34.0 31.0 34.0 3 32.38838016746014 34.0 31.0 34.0 30.0 34.0 4 35.99524225752497 37.0 35.0 37.0 35.0 37.0 5 35.88658922066224 37.0 35.0 37.0 35.0 37.0 6 35.913145098988934 37.0 35.0 37.0 35.0 37.0 7 35.853401464078445 37.0 35.0 37.0 35.0 37.0 8 35.84680116903167 37.0 35.0 37.0 35.0 37.0 9 37.44474725923285 39.0 37.0 39.0 34.0 39.0 10-11 37.42618693875741 39.0 37.0 39.0 34.0 39.0 12-13 37.319473826099745 39.0 37.0 39.0 34.0 39.0 14-15 38.52911983909486 40.0 38.0 41.0 34.0 41.0 16-17 38.48489649168933 40.0 38.0 41.0 34.0 41.0 18-19 38.515323398963645 40.0 38.0 41.0 34.0 41.0 20-21 38.47956357724073 40.0 38.0 41.0 34.0 41.0 22-23 38.34159095237562 40.0 38.0 41.0 34.0 41.0 24-25 38.268027815631285 40.0 38.0 41.0 34.0 41.0 26-27 38.123098482170505 40.0 37.5 41.0 33.0 41.0 28-29 38.10873664298363 40.0 37.5 41.0 33.5 41.0 30-31 38.12251222627785 40.0 37.5 41.0 33.5 41.0 32-33 37.99237265470827 40.0 37.0 41.0 33.0 41.0 34-35 37.852929622834495 40.0 37.0 41.0 33.0 41.0 36-37 37.710749399263506 40.0 37.0 41.0 33.0 41.0 38-39 37.61240494347559 40.0 36.0 41.0 33.0 41.0 40-41 37.50353827301099 40.0 36.0 41.0 32.0 41.0 42-43 37.34401600660935 40.0 36.0 41.0 32.0 41.0 44-45 37.166785451319306 39.0 36.0 41.0 31.5 41.0 46-47 36.89773416090084 39.0 35.0 41.0 31.0 41.0 48-49 36.61765687380564 39.0 35.0 41.0 30.5 41.0 50-51 36.39068014026283 39.0 35.0 40.5 30.0 41.0 52-53 36.161241108134476 38.5 35.0 40.0 29.5 41.0 54-55 35.90250215302149 38.0 35.0 40.0 29.0 41.0 56-57 35.609694150574924 38.0 34.5 40.0 28.5 41.0 58-59 35.30306021047051 37.0 34.0 40.0 28.0 41.0 60-61 35.031848151738615 37.0 34.0 40.0 28.0 41.0 62-63 34.713333323004235 36.0 34.0 39.0 27.0 41.0 64-65 34.33474550010762 35.5 33.0 39.0 26.0 41.0 66-67 34.04012211856838 35.0 33.0 39.0 26.0 40.0 68-69 33.728254006240846 35.0 33.0 38.0 26.0 40.0 70-71 33.456139799922155 35.0 33.0 37.5 26.0 40.0 72-73 33.15748395751689 35.0 32.0 37.0 25.5 39.5 74-75 32.85153781630585 35.0 32.0 36.5 25.0 39.0 76-77 31.889242210328927 34.0 30.5 35.0 24.5 38.0 78-79 32.40752287041563 35.0 32.0 36.0 25.0 38.0 80-81 32.36044767830033 35.0 32.0 35.0 25.0 37.0 82-83 32.13728142928686 35.0 32.0 35.0 25.0 37.0 84-85 31.906529071927963 34.0 31.5 35.0 24.5 36.5 86-87 31.691404623352938 34.0 31.0 35.0 24.0 36.0 88-89 31.492083280992603 34.0 31.0 35.0 24.0 36.0 90-91 31.28686606835751 34.0 31.0 35.0 23.0 35.5 92-93 31.106832175401472 34.0 31.0 35.0 23.0 35.0 94-95 30.931730268895876 34.0 31.0 35.0 21.5 35.0 96-97 30.770375997522926 34.0 30.0 35.0 20.0 35.0 98-99 30.602581969157146 34.0 30.0 35.0 20.0 35.0 100 28.73459734505575 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.34381126644394655 1101 2 -0.337089655589061 1101 3 -0.3939132294486498 1101 4 -0.16422632551606853 1101 5 -0.2631048855524796 1101 6 -0.3406087771275139 1101 7 -0.3180049931449318 1101 8 -0.3392625658367123 1101 9 -0.5222186447060366 1101 10-11 -0.5011694395019148 1101 12-13 -0.5025335417601369 1101 14-15 -0.7282435556861202 1101 16-17 -0.5921074384733416 1101 18-19 -0.7038555618762601 1101 20-21 -0.6979903474170257 1101 22-23 -0.7922966737031061 1101 24-25 -0.6953687448010797 1101 26-27 -0.847337663500916 1101 28-29 -0.8772785680218291 1101 30-31 -0.6797002240085774 1101 32-33 -0.6552285924459227 1101 34-35 -0.668761062464803 1101 36-37 -0.6278107151393755 1101 38-39 -0.7339072832569613 1101 40-41 -0.7833938609955524 1101 42-43 -0.7719896030520417 1101 44-45 -0.7756280054428046 1101 46-47 -0.7632943269682855 1101 48-49 -0.7607375141991355 1101 50-51 -0.8540719640192407 1101 52-53 -0.8485566248195724 1101 54-55 -0.7894224592231396 1101 56-57 -0.7865474383300963 1101 58-59 -0.8395106007178512 1101 60-61 -0.8334255637362489 1101 62-63 -0.9317853987784233 1101 64-65 -0.9034773911054401 1101 66-67 -0.9526072955344489 1101 68-69 -1.1023194142238992 1101 70-71 -1.0440889639313937 1101 72-73 -1.0728281981274392 1101 74-75 -1.0568908908881198 1101 76-77 -0.9640200662463805 1101 78-79 -0.8930712756868715 1101 80-81 -0.839014868078344 1101 82-83 -0.856292097552128 1101 84-85 -0.8100870433297693 1101 86-87 -0.8680727760247358 1101 88-89 -0.9064472154767422 1101 90-91 -1.0522460758781627 1101 92-93 -1.1403653392544513 1101 94-95 -1.1217673947027187 1101 96-97 -1.1796720500004128 1101 98-99 -1.2780586417303006 1101 100 -1.088251661399518 1102 1 -0.4016811571319039 1102 2 -0.37650634009164463 1102 3 -0.402474469904309 1102 4 -0.22454335617660348 1102 5 -0.3065707934053421 1102 6 -0.3627375280867682 1102 7 -0.324214556557024 1102 8 -0.3692864052570428 1102 9 -0.45276827025865884 1102 10-11 -0.4795407054398453 1102 12-13 -0.5864854093677678 1102 14-15 -0.6159074594094847 1102 16-17 -0.6426132974386576 1102 18-19 -0.6483610207359689 1102 20-21 -0.7833955456160666 1102 22-23 -0.754795801092996 1102 24-25 -0.8881738493790294 1102 26-27 -0.9708076562791206 1102 28-29 -0.8881870536072967 1102 30-31 -0.7986035695607825 1102 32-33 -0.6950258624034973 1102 34-35 -0.7304894973192475 1102 36-37 -0.7780870766187604 1102 38-39 -0.8137931536663174 1102 40-41 -0.7760508870301237 1102 42-43 -0.6717264180880491 1102 44-45 -0.740589887284365 1102 46-47 -0.8216446321742055 1102 48-49 -0.7383744227164613 1102 50-51 -0.8814156467619654 1102 52-53 -0.9015996516308391 1102 54-55 -0.9073776301276766 1102 56-57 -0.907827917706939 1102 58-59 -0.8946442574520646 1102 60-61 -0.8716698055990193 1102 62-63 -0.8501887603233342 1102 64-65 -0.8578325439534282 1102 66-67 -0.9532214732676749 1102 68-69 -0.9624182286335241 1102 70-71 -0.924748462299597 1102 72-73 -0.9567852212265961 1102 74-75 -1.1271545914012577 1102 76-77 -0.9369951606177764 1102 78-79 -0.8317384734157613 1102 80-81 -0.8756249424678906 1102 82-83 -0.8347722701648941 1102 84-85 -0.9006302520632836 1102 86-87 -0.9085341012458876 1102 88-89 -0.9459802644385356 1102 90-91 -0.9370064840714001 1102 92-93 -1.0103607897593037 1102 94-95 -1.1056477761169994 1102 96-97 -1.1940430031554143 1102 98-99 -1.26945542554229 1102 100 -1.175722526088915 1103 1 -0.36337715857271746 1103 2 -0.35812892313028044 1103 3 -0.3363522439620681 1103 4 -0.21917785376135868 1103 5 -0.25308076387591427 1103 6 -0.33900069827949864 1103 7 -0.3904037521876731 1103 8 -0.3435012218707527 1103 9 -0.5110147362487325 1103 10-11 -0.5184819750944882 1103 12-13 -0.5715756205750608 1103 14-15 -0.7375543318868836 1103 16-17 -0.7902340598673803 1103 18-19 -0.72448007683154 1103 20-21 -0.7408767162705274 1103 22-23 -0.7740679720274244 1103 24-25 -0.7986578263904818 1103 26-27 -0.7732671118563559 1103 28-29 -0.7860352131704573 1103 30-31 -0.8117164058699089 1103 32-33 -0.764538257503844 1103 34-35 -0.857052645771752 1103 36-37 -0.8749368100163295 1103 38-39 -0.8130129334347842 1103 40-41 -0.8434546791934068 1103 42-43 -0.739740171730837 1103 44-45 -0.6972307129439912 1103 46-47 -0.8572340222007355 1103 48-49 -0.869862202780773 1103 50-51 -0.9482203483393477 1103 52-53 -0.8674532714974674 1103 54-55 -0.9026510263270708 1103 56-57 -0.8302279203981797 1103 58-59 -0.9012440345862913 1103 60-61 -0.8815797620495616 1103 62-63 -0.9236348614750014 1103 64-65 -0.9068149761377029 1103 66-67 -0.9819364870759628 1103 68-69 -0.8411857505711282 1103 70-71 -0.920494446458342 1103 72-73 -0.8316833358009177 1103 74-75 -0.8967669021618576 1103 76-77 -0.9051004493651433 1103 78-79 -0.8169477271377907 1103 80-81 -0.6973516864220421 1103 82-83 -0.7096843549277096 1103 84-85 -0.798252902881476 1103 86-87 -0.7462620296602118 1103 88-89 -0.7876933876925278 1103 90-91 -0.8963464217892785 1103 92-93 -0.9795079633277481 1103 94-95 -0.9990923438185497 1103 96-97 -1.0116983638383488 1103 98-99 -0.9456432221294264 1103 100 -1.1044728983352563 1104 1 -0.393511844632112 1104 2 -0.3385659115283275 1104 3 -0.4026861172302034 1104 4 -0.2500674688863711 1104 5 -0.24308345263017372 1104 6 -0.37702089407496686 1104 7 -0.35528371699987815 1104 8 -0.4204221508431516 1104 9 -0.5177115376794532 1104 10-11 -0.5226980044877081 1104 12-13 -0.61443452106613 1104 14-15 -0.7547834498787722 1104 16-17 -0.6805213890458433 1104 18-19 -0.7502676887067068 1104 20-21 -0.8275385841753788 1104 22-23 -0.7407100411788861 1104 24-25 -0.8038466416469845 1104 26-27 -0.8895384648800544 1104 28-29 -0.9809103299259831 1104 30-31 -0.8782866839098062 1104 32-33 -0.8424665489455947 1104 34-35 -0.8707845892643604 1104 36-37 -0.7657682937001269 1104 38-39 -0.7946350110212919 1104 40-41 -0.8463420012958736 1104 42-43 -0.7885724948625636 1104 44-45 -0.8243616396145015 1104 46-47 -0.9334084459140897 1104 48-49 -0.9723919951326963 1104 50-51 -0.8751532328772313 1104 52-53 -0.8155298949412639 1104 54-55 -0.8373560814034775 1104 56-57 -0.8557761479699977 1104 58-59 -0.8875485708560475 1104 60-61 -0.8753522602429697 1104 62-63 -0.8760272116228194 1104 64-65 -0.9070330045314208 1104 66-67 -0.8094140190678658 1104 68-69 -0.9530656874287615 1104 70-71 -1.1060575676321776 1104 72-73 -1.021436754758824 1104 74-75 -0.9663088417676562 1104 76-77 -0.8922943602134374 1104 78-79 -0.7296103479437548 1104 80-81 -0.7686975218075105 1104 82-83 -0.6702954699595338 1104 84-85 -0.7655614979284415 1104 86-87 -0.7825145910433982 1104 88-89 -0.8895654289474635 1104 90-91 -0.979924029913672 1104 92-93 -0.9742334826382617 1104 94-95 -1.030861232754031 1104 96-97 -1.036767315815105 1104 98-99 -1.0389323248623654 1104 100 -1.2119653813358582 1105 1 -0.36585287485482 1105 2 -0.34570844126842104 1105 3 -0.4201984620627499 1105 4 -0.23415281776517105 1105 5 -0.230902035388155 1105 6 -0.3701015703759225 1105 7 -0.36862757299453364 1105 8 -0.43638805797990443 1105 9 -0.5738305968370696 1105 10-11 -0.5096478494354812 1105 12-13 -0.577765943702353 1105 14-15 -0.8194135187649465 1105 16-17 -0.6979493084661144 1105 18-19 -0.737908361437384 1105 20-21 -0.8058291504429391 1105 22-23 -0.7466023055796995 1105 24-25 -0.7313923141739096 1105 26-27 -0.8594041076851653 1105 28-29 -0.8109558163419095 1105 30-31 -0.7893685825356087 1105 32-33 -0.7235696941037091 1105 34-35 -0.7452539027567084 1105 36-37 -0.6954392631384465 1105 38-39 -0.7227613148710219 1105 40-41 -0.863103544833983 1105 42-43 -0.7727100056771903 1105 44-45 -0.83499381398655 1105 46-47 -0.7049534710099863 1105 48-49 -0.835234847018036 1105 50-51 -0.7518529538728558 1105 52-53 -0.8018250694504374 1105 54-55 -0.8275621216857161 1105 56-57 -0.7782682713905444 1105 58-59 -0.924344485645122 1105 60-61 -0.7801028807347876 1105 62-63 -0.8264935015029451 1105 64-65 -0.8782632572971636 1105 66-67 -0.8110743722648905 1105 68-69 -0.7703570560556088 1105 70-71 -0.7719532643582951 1105 72-73 -0.7577838845904168 1105 74-75 -0.7964189902298706 1105 76-77 -0.8721913853683354 1105 78-79 -0.6908273685518864 1105 80-81 -0.69738236524001 1105 82-83 -0.6387835020533963 1105 84-85 -0.7084048739760753 1105 86-87 -0.7125766001736729 1105 88-89 -0.8069353440407987 1105 90-91 -0.7145976865976031 1105 92-93 -0.6922771369973191 1105 94-95 -0.7098205382431217 1105 96-97 -0.8264669673250538 1105 98-99 -0.8971385734744786 1105 100 -0.9227081208934429 1106 1 -0.41093816958907325 1106 2 -0.42644033518054414 1106 3 -0.4301552606762691 1106 4 -0.253674083622208 1106 5 -0.2960396301957786 1106 6 -0.3678849918862923 1106 7 -0.39276700839103285 1106 8 -0.3718834595788749 1106 9 -0.4919185891727693 1106 10-11 -0.5131724146777898 1106 12-13 -0.5998965073537974 1106 14-15 -0.6798633178228144 1106 16-17 -0.6483918693076234 1106 18-19 -0.7453863618987029 1106 20-21 -0.8056970748156971 1106 22-23 -0.7154385917701802 1106 24-25 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1.0602251741220954 2310 80-81 1.0180986202195115 2310 82-83 1.0553183825677408 2310 84-85 1.1261194344701089 2310 86-87 1.1103756191336096 2310 88-89 1.0996552985759358 2310 90-91 1.163340243674849 2310 92-93 1.2279637097760627 2310 94-95 1.288884202693854 2310 96-97 1.2919305601921458 2310 98-99 1.2740636435211528 2310 100 1.5392717595055458 2311 1 0.3829074754511197 2311 2 0.2856191781434916 2311 3 0.39251091481106926 2311 4 0.2738564645758288 2311 5 0.37866484667038947 2311 6 0.46269763113330953 2311 7 0.4769621825925938 2311 8 0.47371766090407164 2311 9 0.6074508341715656 2311 10-11 0.6544890359932936 2311 12-13 0.6505924441613402 2311 14-15 0.805549955741526 2311 16-17 0.6211192746548662 2311 18-19 0.7703240035498524 2311 20-21 0.7458634671471174 2311 22-23 0.8943187969380588 2311 24-25 0.9749948393032071 2311 26-27 0.33734206792805566 2311 28-29 0.6629133045936371 2311 30-31 0.855727531587327 2311 32-33 0.9447905739018765 2311 34-35 0.9971151333852717 2311 36-37 1.021619282079918 2311 38-39 1.0148328286343329 2311 40-41 0.9906452658720539 2311 42-43 0.9812514222333633 2311 44-45 0.9830967554834942 2311 46-47 0.6041986115465434 2311 48-49 0.8533276200127702 2311 50-51 0.9723636786732541 2311 52-53 0.8873584073071044 2311 54-55 0.9698511086771262 2311 56-57 1.0794599362421238 2311 58-59 1.032467352616159 2311 60-61 1.078552440506222 2311 62-63 1.0957929600070813 2311 64-65 1.1522445342195553 2311 66-67 1.176167274505758 2311 68-69 1.1580852571365199 2311 70-71 1.2130659795485244 2311 72-73 1.2077333345859884 2311 74-75 1.1493680567899531 2311 76-77 1.15718504848547 2311 78-79 0.8892491718893112 2311 80-81 0.8593254266431316 2311 82-83 0.9510427729415554 2311 84-85 1.0175510159461219 2311 86-87 1.092223329601783 2311 88-89 1.0896964272878442 2311 90-91 1.087477225626369 2311 92-93 1.191098339553335 2311 94-95 1.2848585685851255 2311 96-97 1.226893277182075 2311 98-99 1.2711913390414367 2311 100 1.4023680280396036 2312 1 0.27743412410131185 2312 2 0.3788821129257016 2312 3 0.460728034589593 2312 4 0.18017676482499212 2312 5 0.3347825223577914 2312 6 0.4613635946622594 2312 7 0.3332983723235614 2312 8 0.41798686550756514 2312 9 0.5709343234390687 2312 10-11 0.6373603865621575 2312 12-13 0.7176482872229784 2312 14-15 0.9434418239873708 2312 16-17 0.8867785499417167 2312 18-19 0.8491151352187458 2312 20-21 0.8473314194731074 2312 22-23 0.9150479693863787 2312 24-25 0.959808340677796 2312 26-27 1.0255247090544515 2312 28-29 0.9807878853835419 2312 30-31 1.016074800991909 2312 32-33 0.8585096500714684 2312 34-35 0.9630369968750827 2312 36-37 1.0327944647979237 2312 38-39 0.8338559582180167 2312 40-41 0.9564000325951625 2312 42-43 1.0966022490538236 2312 44-45 0.862391226283826 2312 46-47 0.9274418038076746 2312 48-49 1.0400070967324169 2312 50-51 0.8806207206368128 2312 52-53 0.9984373545628102 2312 54-55 0.8390039747647577 2312 56-57 0.8737107190259508 2312 58-59 1.0680024113215794 2312 60-61 1.0921685805636159 2312 62-63 1.147031427769143 2312 64-65 1.1511852023160714 2312 66-67 1.1380252214345816 2312 68-69 1.143820302091811 2312 70-71 1.0651674027047378 2312 72-73 1.0316823911896762 2312 74-75 1.0874043323201477 2312 76-77 1.0792995974089727 2312 78-79 1.0455461688898495 2312 80-81 0.983370462605194 2312 82-83 0.9684798509689543 2312 84-85 1.0313621261490873 2312 86-87 1.0827870372835413 2312 88-89 1.08880581487848 2312 90-91 1.0896431803462328 2312 92-93 1.1384107320642016 2312 94-95 1.1571268284301048 2312 96-97 0.8433785008471162 2312 98-99 0.562527681330856 2312 100 0.9314782342796484 2313 1 0.033908440231066095 2313 2 0.20425237456866085 2313 3 0.26124742155539593 2313 4 0.1431775429976554 2313 5 0.30233051860253113 2313 6 0.2508309633007997 2313 7 0.22032762114920956 2313 8 0.3864051714043981 2313 9 0.6226792800601828 2313 10-11 0.3605348197789837 2313 12-13 0.43708478986244614 2313 14-15 0.45521200087205926 2313 16-17 0.7488817999926027 2313 18-19 0.7154871008030241 2313 20-21 0.651756989982303 2313 22-23 0.6185652951617016 2313 24-25 0.48173890621713866 2313 26-27 0.7173945760910527 2313 28-29 0.5440502620947925 2313 30-31 0.7843281724068092 2313 32-33 0.9102034925498117 2313 34-35 0.9490257812613194 2313 36-37 0.9884263969985554 2313 38-39 0.9933962485502335 2313 40-41 0.9988640904905708 2313 42-43 1.0116970650878656 2313 44-45 0.8208978896770063 2313 46-47 0.8091722054583173 2313 48-49 0.9587082036721952 2313 50-51 0.896928886800886 2313 52-53 0.9553953650869573 2313 54-55 0.8758308413414468 2313 56-57 0.5922855909597402 2313 58-59 0.7164499407942557 2313 60-61 0.6059535398179605 2313 62-63 0.9419744299475852 2313 64-65 1.0492581840400277 2313 66-67 1.0392552211260195 2313 68-69 1.083869805939628 2313 70-71 0.9992995362030186 2313 72-73 0.9795088024289527 2313 74-75 1.0620723529168288 2313 76-77 1.041105085207061 2313 78-79 0.9525520936669523 2313 80-81 0.8718119808563074 2313 82-83 0.9629875279768285 2313 84-85 0.9211552420605464 2313 86-87 0.8997319233000844 2313 88-89 0.9400538430595837 2313 90-91 0.9127194455150622 2313 92-93 1.0385140104483384 2313 94-95 1.1893653678382456 2313 96-97 1.1634970026423233 2313 98-99 1.1742555211290195 2313 100 1.2386203797026916 2314 1 -0.31168493783757256 2314 2 0.04089797223227265 2314 3 0.3136186952395974 2314 4 0.24555026976496208 2314 5 0.34859529477298423 2314 6 0.40864608648433887 2314 7 0.4276670596779013 2314 8 0.4584640259031829 2314 9 0.4473448287654236 2314 10-11 0.5899903569100999 2314 12-13 0.48411137643966384 2314 14-15 0.7859479148107908 2314 16-17 0.7843567865357883 2314 18-19 0.8411947154217998 2314 20-21 0.8766459712741721 2314 22-23 0.9470625239848118 2314 24-25 0.8417918510901217 2314 26-27 0.7855780316237571 2314 28-29 0.8250620196430347 2314 30-31 0.8599247630526676 2314 32-33 0.8692277072321062 2314 34-35 0.8876974558700965 2314 36-37 0.9204783040753668 2314 38-39 0.6633216769570325 2314 40-41 0.6441180630934227 2314 42-43 0.8685943504337956 2314 44-45 0.9269215772481374 2314 46-47 0.8816470944975308 2314 48-49 0.8799223693792655 2314 50-51 0.8431209719624988 2314 52-53 0.8762604960202793 2314 54-55 0.8560523796552459 2314 56-57 0.8123127930966589 2314 58-59 0.9300679566240149 2314 60-61 1.026243197193601 2314 62-63 0.9128884286975207 2314 64-65 0.9450974385115245 2314 66-67 0.7463177999399804 2314 68-69 0.6856076670097195 2314 70-71 0.781222655892428 2314 72-73 0.9740917981135055 2314 74-75 0.9887799361574281 2314 76-77 0.838816995265848 2314 78-79 0.7822083036808394 2314 80-81 0.8238054002851598 2314 82-83 0.7955245384247078 2314 84-85 0.8710505937529938 2314 86-87 0.9314146788257993 2314 88-89 0.9121074432941008 2314 90-91 0.9829118042353677 2314 92-93 0.8973512828302432 2314 94-95 0.9109872128578758 2314 96-97 0.9243101258359729 2314 98-99 1.0657776393703955 2314 100 1.0324071121694551 2315 1 0.40177203846428 2315 2 0.4385027620256352 2315 3 0.4452469344533654 2315 4 0.33311631858256163 2315 5 0.39117483552468 2315 6 0.41467460870475037 2315 7 0.37952990805406017 2315 8 0.29693411791169666 2315 9 0.5132209475195211 2315 10-11 0.5853178472222424 2315 12-13 0.4357889066737144 2315 14-15 0.6813104151765685 2315 16-17 0.7585560752569123 2315 18-19 0.7479830341867739 2315 20-21 0.41996545212210634 2315 22-23 0.8281749396994087 2315 24-25 0.874668956396178 2315 26-27 0.8419269480887266 2315 28-29 0.8035367674661416 2315 30-31 0.9097257021581626 2315 32-33 0.8459437223982462 2315 34-35 0.8639398163628726 2315 36-37 0.8550521951449781 2315 38-39 0.9290656196952156 2315 40-41 0.8963247154216489 2315 42-43 0.9989233247541804 2315 44-45 0.9948368226111342 2315 46-47 1.008074302848108 2315 48-49 0.8418223127361344 2315 50-51 0.8947424338569263 2315 52-53 0.9652494422490179 2315 54-55 0.8085071592068687 2315 56-57 0.7598381048428919 2315 58-59 0.7742989007040748 2315 60-61 0.8967798072589446 2315 62-63 0.9516740553461247 2315 64-65 0.9681532832048276 2315 66-67 0.9303385060352838 2315 68-69 0.953435178223387 2315 70-71 0.8919747136475209 2315 72-73 0.9543707132825787 2315 74-75 0.39658205724414586 2315 76-77 0.4258680305183553 2315 78-79 0.6727180970901756 2315 80-81 0.7903504200789087 2315 82-83 0.7552532314105704 2315 84-85 0.795294454975231 2315 86-87 0.7966510802939233 2315 88-89 0.7924592264031638 2315 90-91 0.8620128923687354 2315 92-93 0.8806440672059352 2315 94-95 0.9195698491253665 2315 96-97 1.0085130409716037 2315 98-99 1.1251386745393006 2315 100 1.0977690534194515 2316 1 0.2873393964140334 2316 2 0.10006461744394812 2316 3 0.23834718231940144 2316 4 -0.006284453851833405 2316 5 0.2521538196993873 2316 6 0.33785126486113626 2316 7 0.43804205002315655 2316 8 0.44946580237650124 2316 9 0.6076673616559205 2316 10-11 0.6222824258578612 2316 12-13 0.40284013599785595 2316 14-15 0.5217377910583991 2316 16-17 0.7793860404170019 2316 18-19 0.7359759495987461 2316 20-21 0.682197396743824 2316 22-23 0.7308138541942952 2316 24-25 0.43875028418916173 2316 26-27 0.7013706755653004 2316 28-29 0.90374846995757 2316 30-31 0.5419389898576696 2316 32-33 0.6786054695622852 2316 34-35 0.8320518429868073 2316 36-37 0.8881957289614419 2316 38-39 0.7554593343293945 2316 40-41 0.7983524738964434 2316 42-43 0.7386231752151673 2316 44-45 0.5994707199066696 2316 46-47 0.8036557322771927 2316 48-49 0.8527162062183393 2316 50-51 0.9412890911321128 2316 52-53 0.9140209059822553 2316 54-55 0.8244991669847863 2316 56-57 0.9129614847712659 2316 58-59 0.7601562760974261 2316 60-61 0.9107541413848779 2316 62-63 0.9151565967845059 2316 64-65 0.9793803697783119 2316 66-67 0.9270666113334443 2316 68-69 0.9568921451814134 2316 70-71 0.9537682048398395 2316 72-73 0.9592865128908414 2316 74-75 0.9344850759152479 2316 76-77 0.8687957333690512 2316 78-79 0.5958055655948016 2316 80-81 0.6172672061089131 2316 82-83 0.5730891072222803 2316 84-85 0.6562100150689609 2316 86-87 0.6829960726278763 2316 88-89 0.6926008866486306 2316 90-91 0.5871601071455252 2316 92-93 0.4349434320196721 2316 94-95 0.4839593943156153 2316 96-97 0.7382022866110987 2316 98-99 0.9233800547733608 2316 100 0.5551636709604217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 10.0 12 35.0 13 180.0 14 665.0 15 2044.0 16 4915.0 17 10506.0 18 18068.0 19 27023.0 20 39604.0 21 56834.0 22 69562.0 23 69121.0 24 67491.0 25 71827.0 26 83697.0 27 102724.0 28 126488.0 29 156035.0 30 193790.0 31 242523.0 32 309088.0 33 411078.0 34 599870.0 35 839607.0 36 1332801.0 37 1887800.0 38 1423617.0 39 243512.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.61979615725217 21.694888335291378 13.641014365620855 23.044301141835604 2 15.312994381525657 19.98039695685638 41.35369691607232 23.352911745545633 3 18.751726648148974 26.681829785803856 29.090818295951127 25.475625270096042 4 12.286387036983385 16.702774800063956 39.58900037878666 31.421837784166 5 12.811008214733413 38.723995397670755 34.627069028026106 13.837927359569724 6 29.745591877704086 38.381990943603824 17.710517590417965 14.161899588274117 7 27.024573786014926 32.216002846094575 23.058359836695193 17.701063531195306 8 26.434908672082138 35.542541154114865 19.476698384155288 18.545851789647717 9 26.28134971387296 15.242293164299259 21.8890105999907 36.58734652183708 10-11 26.094349720543953 26.354900451957757 26.706339726141355 20.84441010135693 12-13 25.814629416271885 24.014129972434407 27.775732483264175 22.395508128029533 14-15 23.256378266720887 25.116714998967883 25.532541233419987 26.094365500891243 16-17 21.583712650670186 27.86478941928693 26.89016474799154 23.661333182051344 18-19 21.027953705208375 27.764700540198067 29.476117341048695 21.73122841354486 20-21 22.689601483004715 26.849617300831785 28.90092321906129 21.55985799710221 22-23 22.97897249448009 26.65484699781921 28.570982490506562 21.795198017194135 24-25 22.295880511601762 26.58656906229882 29.5227270600943 21.594823366005116 26-27 22.233147301090366 26.86129078893093 28.442201346978795 22.463360562999902 28-29 21.43025530253443 27.252108213606885 29.101672650406723 22.21596383345196 30-31 22.399760248280568 26.885981407323285 28.996978849443227 21.71727949495292 32-33 22.705926421930915 27.1341099817892 28.088616953655883 22.071346642624 34-35 22.654741903155873 26.958824684029192 28.89305712130846 21.49337629150648 36-37 22.29687833629217 26.542045790664066 28.579208516300987 22.58186735674278 38-39 22.41171290605721 26.725106504318287 29.11781630056981 21.74536428905469 40-41 22.039416797678975 25.790933554919036 29.76076443253882 22.408885214863165 42-43 22.137805568650684 26.02949315027922 28.939120551998865 22.89358072907123 44-45 22.211662854308393 26.910300805109756 28.7681721235508 22.109864217031046 46-47 22.238959778805185 26.801079033995173 28.08460141483147 22.87535977236817 48-49 23.059065378411304 26.726644531481853 28.018177501489227 22.196112588617613 50-51 22.579910188869416 26.4647350543041 28.040667225612264 22.91468753121422 52-53 22.03692383500257 27.085661730985123 27.76136563490975 23.116048799102558 54-55 22.00721782411001 27.05986753317109 27.65992850116766 23.27298614155124 56-57 22.976532905960774 26.53486949247979 27.34149070015965 23.14710690139978 58-59 21.86316703174171 27.08465698490225 28.127109883963648 22.92506609939239 60-61 22.125675284934903 27.71853511290962 27.348417110420513 22.807372491734963 62-63 22.48795410761712 26.919738863836486 27.035472155598555 23.556834872947835 64-65 21.671048954450594 27.10281259657168 27.42868240936092 23.797456039616804 66-67 22.14905809299571 26.760765441540507 27.418884840255547 23.67129162520824 68-69 21.803653410474865 26.718176508661877 27.654411017175278 23.823759063687977 70-71 21.731877213899534 26.340377526828835 27.83807976063189 24.08966549863974 72-73 22.290019411289265 26.480518449965718 27.925308835883655 23.304153302861362 74-75 22.46510446752234 26.822785240240428 27.4566842344026 23.255426057834633 76-77 22.15876183352484 26.727778677229917 27.087651597756846 24.025807891488398 78-79 22.101900141758925 26.693232434275206 27.182078715688572 24.022788708277297 80-81 21.549643152632846 27.197166006668077 27.6740669301258 23.579123910573273 82-83 22.564040656949537 26.116045187635994 28.676508986855048 22.643405168559422 84-85 22.660818486814765 26.604637324806507 27.39238273878693 23.342161449591796 86-87 21.324614207417895 27.30384545507721 27.873743243870923 23.49779709363397 88-89 22.286904290377084 26.096697982408468 27.718528287750367 23.897869439464085 90-91 22.00898883585728 26.129050798059666 27.830721943603237 24.031238422479817 92-93 22.307168523823343 26.338481022377685 27.637944227831145 23.716406225967827 94-95 21.1510226592962 26.290188012255665 28.317096526204743 24.241692802243396 96-97 21.49291742801728 26.234681164776752 27.848615349996418 24.423786057209547 98-99 20.83753147113239 26.695221156230332 27.798677735604343 24.668569637032938 100 21.538885788432115 26.107803935141334 27.079255663056163 25.27405461337039 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 717.0 1 505.0 2 439.0 3 599.5 4 506.5 5 467.5 6 755.0 7 1507.0 8 2376.5 9 2918.0 10 2926.0 11 2881.5 12 3292.5 13 4051.5 14 5062.0 15 6318.0 16 7611.0 17 8796.0 18 9997.5 19 11125.5 20 12167.5 21 13146.5 22 14543.5 23 16331.0 24 18269.5 25 21079.0 26 25940.5 27 31242.0 28 38246.0 29 52676.0 30 73913.0 31 94596.0 32 123438.5 33 155268.5 34 179333.5 35 205719.0 36 230220.5 37 266807.0 38 282211.0 39 266742.0 40 246451.5 41 224364.0 42 218575.5 43 227214.5 44 248474.5 45 296022.0 46 338301.5 47 363271.5 48 385181.5 49 384029.0 50 368709.5 51 373466.5 52 373503.0 53 350563.0 54 329617.0 55 285746.5 56 246275.5 57 202869.0 58 159110.5 59 153029.0 60 126571.5 61 84826.5 62 62053.0 63 52083.5 64 37816.5 65 17955.5 66 9767.0 67 6729.0 68 5149.5 69 4052.0 70 3280.5 71 2549.0 72 2114.0 73 1870.5 74 1490.5 75 1071.0 76 723.5 77 461.0 78 273.5 79 171.5 80 125.5 81 85.5 82 55.0 83 38.0 84 22.5 85 14.0 86 9.5 87 3.0 88 2.0 89 1.0 90 2.0 91 2.5 92 1.5 93 2.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044991176962038415 2 0.0 3 0.0 4 0.03846000743218489 5 0.0 6 0.0 7 0.0010607191389725612 8 0.0 9 0.010774046085743769 10-11 4.469322214772027E-4 12-13 1.1918192572725407E-5 14-15 1.1918192572725407E-4 16-17 2.6220023659995895E-4 18-19 5.9590962863627034E-6 20-21 1.1918192572725407E-5 22-23 0.0 24-25 7.448870357953379E-4 26-27 6.316642063544465E-4 28-29 2.1452746630905734E-4 30-31 0.002234661107386014 32-33 0.015273163781947608 34-35 0.02645242841516404 36-37 0.03178581959145866 38-39 0.022727993236187353 40-41 0.0012037374498452662 42-43 1.1322282944089137E-4 44-45 0.011113714574066442 46-47 0.018461280295151653 48-49 0.003241748379781311 50-51 0.011060082707489177 52-53 0.010875350722611933 54-55 0.010565477715721075 56-57 4.8268679919537895E-4 58-59 0.0084202030526305 60-61 0.004433567637053851 62-63 0.0014540194938724995 64-65 0.03554005025186716 66-67 0.06108669603150407 68-69 0.06056229555830415 70-71 0.05308958881520533 72-73 0.046713355788797234 74-75 0.035742659525603494 76-77 0.0323221382572313 78-79 0.015392345707674863 80-81 0.0013348375681452457 82-83 0.03913934440883023 84-85 0.05302403875605534 86-87 0.05848853005064994 88-89 0.07071063653397984 90-91 0.04923405351792866 92-93 0.039806763192902855 94-95 0.01319939827429339 96-97 0.0034681940386630935 98-99 0.02257305673274192 100 0.034336312802021894 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8390534.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.98643836859208 #Duplication Level Percentage of deduplicated Percentage of total 1 75.8994216701284 48.56533666907454 2 14.97377774826017 19.162374140680896 3 4.7769697820409736 9.169838476415746 4 1.9345936435125812 4.951510277555511 5 0.9079454133712839 2.904809661736376 6 0.4739547234435375 1.8196004820673812 7 0.275832637720542 1.2354683651486156 8 0.16033342101794526 0.8207331649912225 9 0.11650767313084757 0.6709419941629545 >10 0.4346671946375049 4.622902974193431 >50 0.02436302126509554 1.0946825289938742 >100 0.019053308167399655 2.409665308948243 >500 0.0018806040806512029 0.8204932795681217 >1k 5.786145298999638E-4 0.5942423266967849 >5k 4.821787720790778E-5 0.24108457843374625 >10k+ 7.232681581186167E-5 0.9163157713326141 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24672 0.2940456471542812 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21709 0.2587320425612959 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13018 0.15515103091173935 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 9969 0.11881246175749957 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.9590962863627034E-6 0.0 14-15 5.9590962863627034E-6 0.0 0.0 1.1918192572725407E-5 0.0 16-17 1.787728885908811E-5 0.0 0.0 4.171367400453892E-5 0.0 18-19 2.3836385145450814E-5 0.0 0.0 5.3631866577264335E-5 0.0 20-21 2.3836385145450814E-5 0.0 0.0 1.0726373315452866E-4 0.0 22-23 3.575457771817622E-5 0.0 0.0 3.098730068908606E-4 0.0 24-25 3.575457771817622E-5 0.0 0.0 7.091324580771617E-4 0.0 26-27 3.575457771817622E-5 0.0 0.0 0.001686424249040645 0.0 28-29 4.767277029090163E-5 0.0 0.0 0.0048804998585310535 0.0 30-31 5.9590962863627036E-5 0.0 0.0 0.01853278945058801 0.0 32-33 5.9590962863627036E-5 0.0 0.0 0.05346501188124618 0.0 34-35 5.9590962863627036E-5 0.0 0.0 0.10401006658217463 0.0 36-37 7.150915543635244E-5 0.0 0.0 0.18881396583340226 0.0 38-39 7.150915543635244E-5 0.0 0.0 0.31371662399556455 0.0 40-41 7.150915543635244E-5 0.0 0.0 0.44075859772453096 0.0 42-43 7.150915543635244E-5 0.0 0.0 0.5665074475593568 0.0 44-45 7.150915543635244E-5 0.0 0.0 0.7172129926414695 0.0 46-47 7.150915543635244E-5 0.0 0.0 0.8952946260631326 0.0 48-49 7.150915543635244E-5 0.0 0.0 1.0903537248046429 0.0 50-51 7.150915543635244E-5 0.0 0.0 1.291008414959048 0.0 52-53 7.150915543635244E-5 0.0 0.0 1.477927388173387 0.0 54-55 7.746825172271515E-5 0.0 0.0 1.6810372259977733 0.0 56-57 8.342734800907785E-5 0.0 0.0 1.9148662051783594 0.0 58-59 8.342734800907785E-5 5.9590962863627034E-6 0.0 2.1864222229479076 0.0 60-61 8.938644429544055E-5 1.1918192572725407E-5 0.0 2.460028169839965 0.0 62-63 9.534554058180326E-5 1.1918192572725407E-5 0.0 2.7365183193346216 0.0 64-65 9.534554058180326E-5 1.1918192572725407E-5 0.0 3.014140697123687 0.0 66-67 9.534554058180326E-5 1.1918192572725407E-5 0.0 3.320861342079062 0.0 68-69 9.534554058180326E-5 1.1918192572725407E-5 0.0 3.661280676533818 0.0 70-71 9.534554058180326E-5 1.1918192572725407E-5 0.0 4.026805683643019 0.0 72-73 9.534554058180326E-5 1.1918192572725407E-5 0.0 4.394869265770212 0.0 74-75 9.534554058180326E-5 1.1918192572725407E-5 0.0 4.7432141982858305 0.0 76-77 9.534554058180326E-5 1.1918192572725407E-5 0.0 5.136395371260042 0.0 78-79 9.534554058180326E-5 1.1918192572725407E-5 0.0 5.557941842557339 0.0 80-81 1.0726373315452866E-4 1.1918192572725407E-5 0.0 6.006608161053873 0.0 82-83 1.0726373315452866E-4 1.1918192572725407E-5 0.0 6.460047715675785 0.0 84-85 1.1918192572725407E-4 1.1918192572725407E-5 0.0 6.892785369798871 0.0 86-87 1.1918192572725407E-4 1.1918192572725407E-5 0.0 7.344747068541764 0.0 88 1.1918192572725407E-4 1.1918192572725407E-5 0.0 7.712119395499738 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16695 0.0 46.5551 1 GTATCAA 28665 0.0 37.661777 1 GTACTAG 8825 0.0 35.3248 1 GGGCACC 9215 0.0 34.43735 94 GTGGTAT 5270 0.0 32.47668 1 TAGGCAT 10460 0.0 32.431564 5 CAACGCA 32375 0.0 31.884724 5 ATCAACG 32785 0.0 31.441212 3 CTAGGCA 11900 0.0 29.900934 4 TAACGCC 4235 0.0 29.745222 4 CTAACGC 4205 0.0 29.622091 3 CTGTGCG 12025 0.0 29.382233 9 TATCAAC 36635 0.0 29.340637 2 TCTAACG 4345 0.0 29.31427 2 TACCTGG 3380 0.0 28.645082 2 AACGCAG 37460 0.0 28.484705 6 CGCCTAC 4420 0.0 28.382547 7 TAGTACT 12775 0.0 27.88972 4 ACTGTGC 13540 0.0 27.343845 8 GCATCGT 10740 0.0 27.298098 8 >>END_MODULE