FastQCFastQC Report
Mon 6 Jun 2016
ERR1211183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5206500
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157950.30337078651685395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126320.242619802170364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80170.1539806011716124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82950.047.0308951
GTATCAA144600.039.7693061
CAACGCA170100.032.4457555
TACCTGG37300.032.2977332
ATCAACG170550.032.2223243
GTACTAG33050.032.037321
TATCAAC182050.031.0709632
AACGCAG189950.029.84716
TCAACGC192950.028.8467464
GTACCTG44200.028.320731
GTACATG191300.028.0436761
GCATCGT33150.027.9346628
TAGTACT45050.027.652924
CTGTGCG44500.026.9364839
TACATGG195800.026.8701442
ACCTGGG44350.026.605673
ACGCAGA208850.026.4482847
ACATGGG193600.026.224823
CGCAGAG213400.025.8383228
CTAGTAC49700.025.6334463
Produced by FastQC (version 0.11.5)