##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211183_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5206500 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43024795928167 31.0 31.0 34.0 28.0 34.0 2 31.45112782099299 31.0 31.0 34.0 28.0 34.0 3 31.514482089695573 33.0 31.0 34.0 28.0 34.0 4 35.103932392202054 37.0 35.0 37.0 32.0 37.0 5 35.157757610678956 37.0 35.0 37.0 32.0 37.0 6 35.221259195236726 37.0 35.0 37.0 32.0 37.0 7 35.13374973590704 37.0 35.0 37.0 32.0 37.0 8 35.136463267070006 37.0 35.0 37.0 32.0 37.0 9 36.70799807932392 39.0 37.0 39.0 32.0 39.0 10-11 36.65537770095074 39.0 37.0 39.0 32.0 39.0 12-13 36.55017180447518 39.0 37.0 39.0 32.0 39.0 14-15 37.751642754249495 40.0 37.0 41.0 32.0 41.0 16-17 37.726019878997405 40.0 37.0 41.0 32.0 41.0 18-19 37.73246864496303 40.0 37.0 41.0 32.0 41.0 20-21 37.69675482569865 40.0 37.0 41.0 32.0 41.0 22-23 37.58638845673677 40.0 37.0 41.0 32.0 41.0 24-25 37.49415893594545 40.0 37.0 41.0 31.5 41.0 26-27 37.34740497455104 40.0 37.0 41.0 31.0 41.0 28-29 37.226158743877846 40.0 36.0 41.0 31.0 41.0 30-31 37.0697931431864 39.5 36.0 41.0 30.5 41.0 32-33 36.88717382118506 39.0 36.0 41.0 30.0 41.0 34-35 36.737556996062615 39.0 35.5 41.0 30.0 41.0 36-37 36.62269499663881 39.0 35.0 41.0 30.0 41.0 38-39 36.479107173725154 39.0 35.0 41.0 29.5 41.0 40-41 36.31260011524057 39.0 35.0 40.5 29.0 41.0 42-43 36.10251426101988 39.0 35.0 40.0 28.0 41.0 44-45 35.88258743877845 38.5 35.0 40.0 27.5 41.0 46-47 35.65376154806492 38.0 34.5 40.0 27.0 41.0 48-49 35.4472637088255 38.0 34.0 40.0 26.0 41.0 50-51 34.77229117449342 37.5 33.5 39.5 25.0 40.5 52-53 34.88486564870834 38.0 33.5 40.0 26.0 40.5 54-55 35.36609152021512 38.0 34.0 40.0 26.0 41.0 56-57 35.327270239124175 38.0 34.0 40.0 26.0 41.0 58-59 35.11545231921636 38.0 34.0 40.0 26.0 41.0 60-61 34.88936992221262 37.0 34.0 40.0 26.0 41.0 62-63 34.59380774032459 37.0 33.5 40.0 26.0 41.0 64-65 34.33797474310958 36.0 33.0 40.0 25.0 41.0 66-67 34.158407663497556 36.0 33.0 39.0 25.0 41.0 68-69 33.864172380678 35.5 33.0 39.0 25.0 41.0 70-71 33.55412493997887 35.0 33.0 39.0 24.5 41.0 72-73 33.211746950926724 35.0 32.0 38.0 24.0 40.0 74-75 32.87524527033516 35.0 32.0 37.0 23.5 39.5 76-77 32.543389513108615 35.0 32.0 37.0 23.0 39.0 78-79 32.2101108230097 35.0 31.5 36.0 22.0 39.0 80-81 31.913212426774223 35.0 31.0 36.0 21.5 38.0 82-83 31.62373715547873 35.0 31.0 35.0 20.0 37.0 84-85 31.37441678670892 34.5 31.0 35.0 20.0 37.0 86-87 31.147630942091617 34.0 31.0 35.0 20.0 36.0 88-89 30.937175453759725 34.0 30.0 35.0 19.5 36.0 90-91 30.716591472198214 34.0 30.0 35.0 19.0 36.0 92-93 30.521128109094402 34.0 30.0 35.0 18.0 35.5 94-95 30.34812906943244 34.0 29.5 35.0 18.0 35.0 96-97 30.170087198693942 34.0 29.0 35.0 18.0 35.0 98-99 29.936629405550754 34.0 29.0 35.0 17.0 35.0 100 28.808808796696436 33.0 26.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.43690126358563575 1101 2 -0.3667962818611805 1101 3 -0.4001334857799357 1101 4 -0.329063867202521 1101 5 -0.2790573027629648 1101 6 -0.17933444740978643 1101 7 -0.24843608473507572 1101 8 -0.29722182908428607 1101 9 -0.5618036934158965 1101 10-11 -0.49299468061231977 1101 12-13 -0.4344753183343357 1101 14-15 -0.575875687649777 1101 16-17 -0.6473880379822106 1101 18-19 -0.6876141371505469 1101 20-21 -0.6793901495259789 1101 22-23 -0.6548982271519819 1101 24-25 -0.7820092801093423 1101 26-27 -0.8720813648280128 1101 28-29 -0.8034056653740365 1101 30-31 -0.7605615704037803 1101 32-33 -0.897945998283106 1101 34-35 -0.8942183633930298 1101 36-37 -0.9848985892635582 1101 38-39 -1.102118715863213 1101 40-41 -1.152389314435034 1101 42-43 -1.1941485835701684 1101 44-45 -1.0873722363214 1101 46-47 -1.1846611710970691 1101 48-49 -1.2260780796262196 1101 50-51 -1.090687007617099 1101 52-53 -1.0803235587086064 1101 54-55 -0.9990510426462436 1101 56-57 -1.0209967082833202 1101 58-59 -0.9883799438132073 1101 60-61 -0.9387944928323932 1101 62-63 -0.9376931876462251 1101 64-65 -0.9141397317550428 1101 66-67 -0.9892864070002076 1101 68-69 -0.8995527832129611 1101 70-71 -1.038341103045802 1101 72-73 -1.0578482798148272 1101 74-75 -1.0745661167479454 1101 76-77 -1.0599505198336772 1101 78-79 -1.0581404115898962 1101 80-81 -1.102481139382519 1101 82-83 -1.1715882047926023 1101 84-85 -1.3380481237434232 1101 86-87 -1.2765263472516821 1101 88-89 -1.3924853486231896 1101 90-91 -1.3843361212454468 1101 92-93 -1.5494926995022489 1101 94-95 -1.4981131512284946 1101 96-97 -1.5930988196374685 1101 98-99 -1.7824066810567984 1101 100 -1.8101760402678728 1102 1 -0.5481371964256105 1102 2 -0.5118255864328418 1102 3 -0.49869754797386534 1102 4 -0.3960182609570069 1102 5 -0.39834971668165053 1102 6 -0.4033643801007756 1102 7 -0.4311442722659393 1102 8 -0.510045487571297 1102 9 -0.6311309159972893 1102 10-11 -0.6876947053508431 1102 12-13 -0.6698155373760031 1102 14-15 -0.7393112106947726 1102 16-17 -0.7935208047512958 1102 18-19 -0.8291954528536252 1102 20-21 -0.8517926277924133 1102 22-23 -0.9030482409926748 1102 24-25 -0.887242971768039 1102 26-27 -0.830073171687296 1102 28-29 -0.9352825604229267 1102 30-31 -0.9159481994629886 1102 32-33 -1.0216748383407435 1102 34-35 -1.0403734086258893 1102 36-37 -0.9862928440408965 1102 38-39 -1.050850544775649 1102 40-41 -1.1531390111812314 1102 42-43 -1.0994405119149064 1102 44-45 -1.3463352830404176 1102 46-47 -1.2357535975229652 1102 48-49 -1.2394904735333725 1102 50-51 -1.1937362142830708 1102 52-53 -1.1189148700617508 1102 54-55 -1.0076296076832634 1102 56-57 -0.9190179944473158 1102 58-59 -0.8591729927485758 1102 60-61 -0.9201679037962478 1102 62-63 -0.9414676744138788 1102 64-65 -1.045855688469068 1102 66-67 -0.97661106468437 1102 68-69 -0.9584321649890413 1102 70-71 -1.0263498866002934 1102 72-73 -1.117834411528598 1102 74-75 -1.091468343490746 1102 76-77 -1.1329225588672003 1102 78-79 -1.1305441083894294 1102 80-81 -1.097060069519646 1102 82-83 -1.2164842294318028 1102 84-85 -1.1801220486983404 1102 86-87 -1.2008196670232785 1102 88-89 -1.1814844317345035 1102 90-91 -1.3410645621760224 1102 92-93 -1.2260229241813612 1102 94-95 -1.3366582043059552 1102 96-97 -1.4078540140371842 1102 98-99 -1.407338006370189 1102 100 -1.5028178936153438 1103 1 -0.4842375758759374 1103 2 -0.3959917938020894 1103 3 -0.4255898091657073 1103 4 -0.3839023484213726 1103 5 -0.36926444281672843 1103 6 -0.42293703035285546 1103 7 -0.3679287398872404 1103 8 -0.42998257265791295 1103 9 -0.5728477079108245 1103 10-11 -0.5924266832268401 1103 12-13 -0.6097322146418449 1103 14-15 -0.675859051820261 1103 16-17 -0.7721903261280616 1103 18-19 -0.6086522276671644 1103 20-21 -0.6903330026851009 1103 22-23 -0.7215308106557288 1103 24-25 -0.7513879189665076 1103 26-27 -0.6956206762891028 1103 28-29 -0.7512584261102333 1103 30-31 -0.7700610532544943 1103 32-33 -0.8626948230779448 1103 34-35 -0.941727673555711 1103 36-37 -0.9673788660306428 1103 38-39 -0.954019719029553 1103 40-41 -1.001186776345996 1103 42-43 -0.9335755179740985 1103 44-45 -0.9617689569604551 1103 46-47 -1.0096472648715107 1103 48-49 -1.0412070755450955 1103 50-51 -1.022866954175882 1103 52-53 -0.9992782052322724 1103 54-55 -0.7456681780670635 1103 56-57 -0.7878918501784611 1103 58-59 -0.8640079371559182 1103 60-61 -0.8834917022918702 1103 62-63 -0.7843215365751917 1103 64-65 -0.7390239264628562 1103 66-67 -0.7575428466523491 1103 68-69 -0.7172585805966776 1103 70-71 -0.8456859324381725 1103 72-73 -0.8201869196457992 1103 74-75 -0.8479072737164017 1103 76-77 -0.8781918175611736 1103 78-79 -0.8383012442814035 1103 80-81 -0.8926623850852344 1103 82-83 -0.8467647806593597 1103 84-85 -0.9608287528507411 1103 86-87 -0.945139239982673 1103 88-89 -0.9119263995059583 1103 90-91 -1.0632950429190942 1103 92-93 -1.0177095967835612 1103 94-95 -1.041593067982948 1103 96-97 -1.1720841520737224 1103 98-99 -1.2512388458245454 1103 100 -1.3709846079782828 1104 1 -0.6045099561674014 1104 2 -0.5151911024097977 1104 3 -0.5831401857046323 1104 4 -0.5250851689935061 1104 5 -0.4681701667224587 1104 6 -0.4681104567285743 1104 7 -0.5512328368363768 1104 8 -0.4896649562906745 1104 9 -0.5662759470451704 1104 10-11 -0.5605421573060525 1104 12-13 -0.5574031029138737 1104 14-15 -0.8825843840226852 1104 16-17 -0.852693191936261 1104 18-19 -0.9440899381048808 1104 20-21 -0.8893725392723368 1104 22-23 -0.8612678759653107 1104 24-25 -0.8773164151240067 1104 26-27 -0.8428122163718683 1104 28-29 -0.8223992233960686 1104 30-31 -0.916298394863972 1104 32-33 -1.0395733410824164 1104 34-35 -1.1206816535593092 1104 36-37 -1.1435385223906849 1104 38-39 -1.1148656143697195 1104 40-41 -1.1613716402522343 1104 42-43 -1.126886650932171 1104 44-45 -1.0915479813005433 1104 46-47 -1.128901190900244 1104 48-49 -1.1193284611569396 1104 50-51 -1.1623526187092565 1104 52-53 -1.000787413086897 1104 54-55 -0.8543022284950013 1104 56-57 -0.8913468839770076 1104 58-59 -0.9244549646831004 1104 60-61 -0.8795536255961522 1104 62-63 -0.9906918555409305 1104 64-65 -0.8338823388308114 1104 66-67 -0.9174899641376371 1104 68-69 -0.979150720828514 1104 70-71 -0.9267341692593192 1104 72-73 -0.8843310646582623 1104 74-75 -0.8951039542623178 1104 76-77 -0.9032780809585041 1104 78-79 -0.9407619197382608 1104 80-81 -0.9560410830059176 1104 82-83 -0.9255118570209397 1104 84-85 -0.8755042932419279 1104 86-87 -0.855201214691597 1104 88-89 -1.082287774065808 1104 90-91 -1.159757440431111 1104 92-93 -1.1681211551149673 1104 94-95 -1.1615289085771856 1104 96-97 -1.34061905581855 1104 98-99 -1.4024793038398116 1104 100 -1.247237595872491 1105 1 -0.5623436901536749 1105 2 -0.5377958152421378 1105 3 -0.5604897741002084 1105 4 -0.33893514181760764 1105 5 -0.27177744030847606 1105 6 -0.3935639066253245 1105 7 -0.3597797240469376 1105 8 -0.4015596165649242 1105 9 -0.5929112104530745 1105 10-11 -0.61435507536644 1105 12-13 -0.6163542935606827 1105 14-15 -0.775549589860951 1105 16-17 -0.7897728105543251 1105 18-19 -0.7707341700094759 1105 20-21 -0.7660267762335451 1105 22-23 -0.7098617824351621 1105 24-25 -0.7892867442723457 1105 26-27 -0.706904766750931 1105 28-29 -0.8378418480018368 1105 30-31 -0.8515511892943124 1105 32-33 -0.8339959050990018 1105 34-35 -0.7667631415906513 1105 36-37 -0.8184394407827682 1105 38-39 -0.8265980304827352 1105 40-41 -0.9291935980164681 1105 42-43 -0.9150715578986706 1105 44-45 -0.7991097309776336 1105 46-47 -0.9667282142877411 1105 48-49 -0.9953383859928238 1105 50-51 -0.9264551966788446 1105 52-53 -0.8214031251720186 1105 54-55 -0.7622696336430081 1105 56-57 -0.6362388518995346 1105 58-59 -0.6442222750084099 1105 60-61 -0.7101602429946823 1105 62-63 -0.7010849911707382 1105 64-65 -0.738451585089507 1105 66-67 -0.6419306048578548 1105 68-69 -0.6798510747911948 1105 70-71 -0.6722681959160326 1105 72-73 -0.7564880061394987 1105 74-75 -0.7897495138649653 1105 76-77 -0.796393024637247 1105 78-79 -0.8198552272567454 1105 80-81 -0.7777374538744404 1105 82-83 -0.8644052752259981 1105 84-85 -0.8417601667059067 1105 86-87 -0.9779208955393024 1105 88-89 -0.9749057323312016 1105 90-91 -1.0336314659521157 1105 92-93 -1.018987922012471 1105 94-95 -1.0184292879581172 1105 96-97 -1.1068615666731318 1105 98-99 -1.269955636472794 1105 100 -1.3220722645654064 1106 1 -0.5095597515071688 1106 2 -0.4809193031919925 1106 3 -0.5524715061829752 1106 4 -0.4202569604044868 1106 5 -0.4594380292244722 1106 6 -0.41289876243229173 1106 7 -0.4992963934993355 1106 8 -0.464032775846384 1106 9 -0.6022214981016774 1106 10-11 -0.5086484488084082 1106 12-13 -0.6089030340975725 1106 14-15 -0.7827142318496598 1106 16-17 -0.8074637613599407 1106 18-19 -0.7712998095179486 1106 20-21 -0.6954499444904343 1106 22-23 -0.7764024545830495 1106 24-25 -0.7666959616483311 1106 26-27 -0.7847803971559202 1106 28-29 -0.8588576365818739 1106 30-31 -0.959120928794988 1106 32-33 -0.9255442554863791 1106 34-35 -1.0342792979092295 1106 36-37 -0.9705854286790796 1106 38-39 -0.9187679484321052 1106 40-41 -0.9951565751023068 1106 42-43 -0.8528510132481912 1106 44-45 -0.9649010378636689 1106 46-47 -0.8886959500052782 1106 48-49 -0.8128518816993022 1106 50-51 -0.9654058071071603 1106 52-53 -0.894508929435311 1106 54-55 -0.7404022946326734 1106 56-57 -0.7293609598638611 1106 58-59 -0.7275450063729139 1106 60-61 -0.6454263362203179 1106 62-63 -0.7511083184461214 1106 64-65 -0.7681638216751097 1106 66-67 -0.6544081248935925 1106 68-69 -0.7213615694155422 1106 70-71 -0.6442313500816113 1106 72-73 -0.6650415318038085 1106 74-75 -0.604011388309246 1106 76-77 -0.562113742503513 1106 78-79 -0.5099401085106479 1106 80-81 -0.40982348636746835 1106 82-83 -0.5310334053367747 1106 84-85 -0.5808064708507423 1106 86-87 -0.7130970357882234 1106 88-89 -0.8526172438172068 1106 90-91 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1107 48-49 -0.9078309031064293 1107 50-51 -0.9810462170426391 1107 52-53 -0.7875928997969623 1107 54-55 -0.7119492927856754 1107 56-57 -0.8185819355560469 1107 58-59 -0.8233333339813171 1107 60-61 -0.9407645798772464 1107 62-63 -0.8619242001153538 1107 64-65 -0.7809724293488571 1107 66-67 -0.8030500015345083 1107 68-69 -0.7709051654932679 1107 70-71 -0.8190956245978711 1107 72-73 -0.7385746661585486 1107 74-75 -0.698331155390278 1107 76-77 -0.6617190542134068 1107 78-79 -0.6771585675137253 1107 80-81 -0.839263816061667 1107 82-83 -0.7840543429489237 1107 84-85 -0.8221803642023495 1107 86-87 -0.8859536339220675 1107 88-89 -0.8074628653549247 1107 90-91 -0.8571750633616162 1107 92-93 -0.8771296905786556 1107 94-95 -0.8387010525909346 1107 96-97 -0.9934976922372627 1107 98-99 -0.9384344627076615 1107 100 -1.0170208633895363 1108 1 -0.6022395546452408 1108 2 -0.5164630671199006 1108 3 -0.6612800023346814 1108 4 -0.47045079918481747 1108 5 -0.3818620461144775 1108 6 -0.4183992891637729 1108 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1.288862726548114 2310 92-93 1.2929090671699441 2310 94-95 1.3412219622345312 2310 96-97 1.3797618182159894 2310 98-99 1.4297853251043442 2310 100 1.4697251208893434 2311 1 0.6408444780854303 2311 2 0.6348632866219539 2311 3 0.6831160603160278 2311 4 0.5305873764131803 2311 5 0.5743069921340123 2311 6 0.5665103836635197 2311 7 0.6226194252298924 2311 8 0.5425137209590716 2311 9 0.7418336994429922 2311 10-11 0.7250822366527743 2311 12-13 0.8106386022806973 2311 14-15 1.0163350487790126 2311 16-17 1.085015407155261 2311 18-19 0.9840617712968296 2311 20-21 1.0019294762153166 2311 22-23 1.00637406703364 2311 24-25 1.0217242259128838 2311 26-27 1.0169805794155948 2311 28-29 1.0326440937836665 2311 30-31 1.0550144140623559 2311 32-33 1.0853399008188518 2311 34-35 1.0537507066209457 2311 36-37 1.1313365150123218 2311 38-39 1.1526476397929741 2311 40-41 1.2487613119641665 2311 42-43 1.2278357878337616 2311 44-45 1.2829650765666827 2311 46-47 1.3409732193696655 2311 48-49 1.3021845663379281 2311 50-51 1.3176338205921354 2311 52-53 1.2192429367802475 2311 54-55 1.2381278391341226 2311 56-57 1.117834288306291 2311 58-59 1.1099424419668722 2311 60-61 1.072474067251484 2311 62-63 1.1365463487323524 2311 64-65 1.0558612649740624 2311 66-67 1.1217563343709642 2311 68-69 1.1388176547899036 2311 70-71 1.0576171023303758 2311 72-73 1.0402741789618517 2311 74-75 1.0363296579343242 2311 76-77 1.0617153777339539 2311 78-79 1.0493342284833815 2311 80-81 1.066620708896135 2311 82-83 1.086273604965232 2311 84-85 1.1180496643897406 2311 86-87 1.1340721377200822 2311 88-89 1.1051447317893093 2311 90-91 1.1399051732121563 2311 92-93 1.1231335160423086 2311 94-95 1.1443794444981847 2311 96-97 1.2160567619292593 2311 98-99 1.2493413929022452 2311 100 1.3852950319220483 2312 1 0.6182036543077878 2312 2 0.6116356879882083 2312 3 0.6187663083717077 2312 4 0.375756263431235 2312 5 0.49752688962125546 2312 6 0.5602167748275093 2312 7 0.3905181367755688 2312 8 0.4762336503885649 2312 9 0.7010714434621335 2312 10-11 0.6648026609684408 2312 12-13 0.8092065336127234 2312 14-15 0.996058143335965 2312 16-17 1.0422204149570504 2312 18-19 1.0535639348376833 2312 20-21 1.043891563164756 2312 22-23 1.0372625835302784 2312 24-25 1.0915920845215865 2312 26-27 1.159439291862661 2312 28-29 1.0871199968524863 2312 30-31 1.1220924667136956 2312 32-33 1.1278629736288153 2312 34-35 1.1295108721343325 2312 36-37 1.2006364140660466 2312 38-39 1.2089559562873404 2312 40-41 1.3244147936262465 2312 42-43 1.3319604573626904 2312 44-45 1.2629259604307705 2312 46-47 1.3912150506847993 2312 48-49 1.3580047515680036 2312 50-51 1.340461320598699 2312 52-53 1.1853776919669272 2312 54-55 1.1817797589074388 2312 56-57 1.1738234830726668 2312 58-59 1.1048706790956757 2312 60-61 1.077782169995281 2312 62-63 1.05116352408308 2312 64-65 1.1345531716379114 2312 66-67 1.0987792964769554 2312 68-69 1.0463103124468134 2312 70-71 1.058401301109214 2312 72-73 1.0452130314210137 2312 74-75 1.1112426426361708 2312 76-77 1.1648125622647996 2312 78-79 1.124013881572985 2312 80-81 1.09237675035806 2312 82-83 1.1416846120914137 2312 84-85 1.1712595821099931 2312 86-87 1.100021310590698 2312 88-89 0.9865245956540392 2312 90-91 1.040446999189001 2312 92-93 1.127500963120994 2312 94-95 1.225736314314677 2312 96-97 1.250360282928451 2312 98-99 0.9683462745977565 2312 100 1.2314930721552884 2313 1 0.6343207201318073 2313 2 0.659683621075299 2313 3 0.5993223885618484 2313 4 0.5736534697507238 2313 5 0.34968945987573363 2313 6 0.4536969267758053 2313 7 0.5508353129343462 2313 8 0.5709780243245888 2313 9 0.7122019744366384 2313 10-11 0.7168249062516452 2313 12-13 0.8489815175281947 2313 14-15 0.9712470395865083 2313 16-17 0.9492254039540242 2313 18-19 0.9508573683734838 2313 20-21 0.9942686408567241 2313 22-23 1.052054221174366 2313 24-25 0.9870334476445564 2313 26-27 0.9884932218805247 2313 28-29 1.0245199401231204 2313 30-31 1.1088918220723656 2313 32-33 1.1700839151955051 2313 34-35 1.1825281449405978 2313 36-37 1.195096219846583 2313 38-39 1.2005739325399034 2313 40-41 1.1551469630703153 2313 42-43 1.2644138950941581 2313 44-45 1.2082101026129806 2313 46-47 1.2019352388691544 2313 48-49 1.22838961962033 2313 50-51 1.2278150686685123 2313 52-53 1.1360116111631058 2313 54-55 0.9977098730264444 2313 56-57 0.9950176897724816 2313 58-59 0.9556586978995014 2313 60-61 0.9539075773769099 2313 62-63 0.9431368054715037 2313 64-65 0.9566799527238103 2313 66-67 0.9107839172148502 2313 68-69 0.9413446844401463 2313 70-71 0.916205925881826 2313 72-73 1.00428437207092 2313 74-75 1.0179210565287633 2313 76-77 0.8296234256266004 2313 78-79 0.9166450044359138 2313 80-81 0.9952998483873507 2313 82-83 1.0275264991747655 2313 84-85 1.0460876771368213 2313 86-87 1.070450425327138 2313 88-89 1.1041444761580799 2313 90-91 1.2053538720044976 2313 92-93 1.2668831408965602 2313 94-95 1.2238925436298906 2313 96-97 1.352405346507016 2313 98-99 1.3554956269727612 2313 100 1.4562994263953044 2314 1 0.5694042074000052 2314 2 0.55046525005638 2314 3 0.5381876175565026 2314 4 0.440133004304478 2314 5 0.42700088989274576 2314 6 0.43189781567649277 2314 7 0.5208585223272522 2314 8 0.5241374529980192 2314 9 0.6723361051624721 2314 10-11 0.6752405896245577 2314 12-13 0.7194540724152958 2314 14-15 0.8191258252671147 2314 16-17 0.8583251723951335 2314 18-19 0.8297297623456927 2314 20-21 0.9004599747734119 2314 22-23 0.9507078850041921 2314 24-25 0.9146588013757722 2314 26-27 0.9102141957793393 2314 28-29 1.0068558550245612 2314 30-31 0.9407296521918056 2314 32-33 0.9191348334812375 2314 34-35 0.9911414825563511 2314 36-37 1.0074564569323883 2314 38-39 0.9534896017521248 2314 40-41 1.104125745409469 2314 42-43 1.0656472723860944 2314 44-45 1.1084672176293822 2314 46-47 1.1200412225566012 2314 48-49 1.0540815921059306 2314 50-51 1.063838998469123 2314 52-53 1.0215255642842322 2314 54-55 0.8810087126545483 2314 56-57 0.8975914354210275 2314 58-59 0.8697051149875747 2314 60-61 0.8330649087239905 2314 62-63 0.9331923316038484 2314 64-65 0.8911087939938298 2314 66-67 0.8952009656222302 2314 68-69 0.9121421276127322 2314 70-71 0.9853781713609351 2314 72-73 0.9639849231341984 2314 74-75 1.0085680521500535 2314 76-77 0.9987771528773024 2314 78-79 0.9142060979338282 2314 80-81 0.9416918021900962 2314 82-83 0.9898031006973476 2314 84-85 0.9277232974109175 2314 86-87 1.0432128354276244 2314 88-89 1.071931183105427 2314 90-91 1.1304218246938298 2314 92-93 1.1668794213024114 2314 94-95 1.1940554950367854 2314 96-97 1.243615796333792 2314 98-99 1.31472725451205 2314 100 1.3798137668584012 2315 1 0.4302250117385178 2315 2 0.4348054519986988 2315 3 0.47535804522452096 2315 4 0.22228947057158166 2315 5 0.3172835675975989 2315 6 0.36566829943693335 2315 7 0.4178833449033519 2315 8 0.4164236442903473 2315 9 0.5391823907453954 2315 10-11 0.5143106137708529 2315 12-13 0.5507094421242087 2315 14-15 0.6884153844015088 2315 16-17 0.6810159612775664 2315 18-19 0.5498611457139404 2315 20-21 0.6779272748660432 2315 22-23 0.582915995621498 2315 24-25 0.7597178641043243 2315 26-27 0.8515474404792727 2315 28-29 0.8582322052863987 2315 30-31 0.8709046282018633 2315 32-33 0.8446123654821989 2315 34-35 0.8823516689164066 2315 36-37 1.025553279785889 2315 38-39 1.00109556743611 2315 40-41 1.0125992296754873 2315 42-43 1.0741171880978015 2315 44-45 1.1667303527800001 2315 46-47 1.1345361652413075 2315 48-49 1.1490923821876393 2315 50-51 1.074106907810382 2315 52-53 1.0607987789855997 2315 54-55 0.9697649677510611 2315 56-57 0.86792967001481 2315 58-59 0.875440455604938 2315 60-61 0.9178406358112525 2315 62-63 0.8980803439844607 2315 64-65 0.9033848984418356 2315 66-67 0.890183929056235 2315 68-69 0.9608028358031859 2315 70-71 0.9432858492266831 2315 72-73 0.643172117505749 2315 74-75 0.8630949008397408 2315 76-77 0.9455502977344352 2315 78-79 0.9030652429813983 2315 80-81 0.7307889046981337 2315 82-83 0.7234256888284598 2315 84-85 0.9171897907175186 2315 86-87 1.0429697371290203 2315 88-89 1.060422065461779 2315 90-91 1.1254427324093754 2315 92-93 1.1993586410983283 2315 94-95 1.2313244137943578 2315 96-97 1.131484223382941 2315 98-99 0.575806107726077 2315 100 1.1415599447764748 2316 1 0.30814868247408 2316 2 0.2909763751657586 2316 3 0.3083887134994754 2316 4 0.16824192950866745 2316 5 0.10750156016800361 2316 6 0.16326589585167284 2316 7 0.08448817568714873 2316 8 0.20515474773437603 2316 9 0.2632439927174346 2316 10-11 0.2820064577834387 2316 12-13 0.0842062479183312 2316 14-15 0.07096197542892213 2316 16-17 0.31887075507093243 2316 18-19 0.3520044243781939 2316 20-21 0.41737918169883415 2316 22-23 0.3513346301539073 2316 24-25 0.40374233374232205 2316 26-27 0.4080447328806329 2316 28-29 0.34046703893145747 2316 30-31 0.3248291438847417 2316 32-33 0.5795940858963462 2316 34-35 0.5215113286104938 2316 36-37 0.4842955783274121 2316 38-39 0.6521695928404796 2316 40-41 0.6672118507200508 2316 42-43 0.6369578977525521 2316 44-45 0.5425795041311119 2316 46-47 0.43017281426062937 2316 48-49 0.4232553404687991 2316 50-51 0.4039048395579101 2316 52-53 0.5292045186280916 2316 54-55 0.2859857570506392 2316 56-57 0.19800814830973223 2316 58-59 0.20632374723124514 2316 60-61 0.3199738247985806 2316 62-63 0.46202475723742253 2316 64-65 0.42645239706750715 2316 66-67 0.3371267215980396 2316 68-69 0.3119253098364112 2316 70-71 0.11988873933838562 2316 72-73 0.07314579546770261 2316 74-75 0.3117839700231144 2316 76-77 0.388740859109312 2316 78-79 0.34374006360179266 2316 80-81 0.647073761931221 2316 82-83 0.6600276734951827 2316 84-85 0.705958250710637 2316 86-87 0.6572713719091752 2316 88-89 0.5623429672425608 2316 90-91 0.43746369368794547 2316 92-93 0.5289278002787867 2316 94-95 0.625707274008704 2316 96-97 0.5663207165829114 2316 98-99 0.44235480395095195 2316 100 0.2077833473932671 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 16.0 1 352.0 2 111.0 3 109.0 4 91.0 5 74.0 6 105.0 7 513.0 8 1698.0 9 3138.0 10 4676.0 11 6809.0 12 9566.0 13 11803.0 14 13528.0 15 15412.0 16 18157.0 17 22410.0 18 27114.0 19 32151.0 20 39373.0 21 47035.0 22 51955.0 23 53206.0 24 52674.0 25 54273.0 26 59567.0 27 68451.0 28 80135.0 29 95746.0 30 116905.0 31 145510.0 32 185825.0 33 243072.0 34 343852.0 35 480108.0 36 724618.0 37 1024534.0 38 917517.0 39 254023.0 40 288.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.008650203504246 19.706348152434376 12.792104354370078 23.492897289691303 2 15.842565725503711 19.68968854234618 41.00945650300934 23.458289229140767 3 19.51685377501931 25.574623162734035 28.846619688033858 26.061903374212797 4 12.708340537030846 15.9388242797263 38.319175666486096 33.03365951675676 5 13.40548089677622 37.53616102859744 34.5544310046902 14.503927069936134 6 31.013503515679798 36.87877966805219 17.874469381205767 14.233247435062252 7 27.744192987172866 31.853749121518092 22.327632577500985 18.074425313808053 8 26.18773242880062 35.41745572119055 19.905465263057568 18.489346586951264 9 26.393286324590896 15.642620832822548 20.996087557849844 36.96800528473671 10-11 25.789237803867543 25.954461239310266 27.425505106910553 20.830795849911638 12-13 25.80550483465263 23.609250775352507 28.017831810617427 22.56741257937744 14-15 23.07860398956451 24.957355246286838 25.784364265871968 26.179676498276685 16-17 22.179849614007235 27.73079362786325 26.80805519045294 23.28130156767658 18-19 21.760243293498714 27.263661798830846 29.032298034151722 21.94379687351871 20-21 22.933657458716375 26.541876857328546 28.659786110289847 21.864679573665235 22-23 23.032727258056507 26.360244884087543 28.412940129657965 22.19408772819799 24-25 22.56690739310196 26.309777286902737 28.94747711766868 22.175838202326627 26-27 22.510508864061023 26.570542445936606 28.37256421662029 22.546384473382076 28-29 22.043725355573237 26.86571493666937 28.700213119181917 22.390346588575476 30-31 22.65825474208097 26.63105263420768 28.635288051811635 22.07540457189971 32-33 22.717953116818734 26.792031851385307 28.102907431167022 22.387107600628937 34-35 22.81539292789818 26.728376058113707 28.4747553581077 21.981475655880413 36-37 22.608229906856618 26.54982183571078 28.206765094399795 22.635183163032814 38-39 22.67005903766767 26.694625423514072 28.483784788716264 22.151530750101987 40-41 22.600728575473955 26.116589366148556 28.786406200230306 22.49627585814718 42-43 22.51600140945356 26.332227570648474 28.3308811351865 22.820889884711463 44-45 22.552596616112314 26.86088507634456 28.275198373068474 22.311319934474657 46-47 22.56935126814722 26.68238321613176 27.784934037666876 22.96333147805414 48-49 22.928751742268403 26.67065622272752 27.791270577212995 22.609321457791086 50-51 22.72106502794932 26.693527137557755 27.74632272737621 22.83908510711672 52-53 22.476523837009562 27.06875760410174 27.520680466710267 22.93403809217843 54-55 22.420416897312393 26.962349202848024 27.59994012978157 23.01729377005801 56-57 22.92113274941613 26.770461359917995 27.3255189494486 22.982886941217274 58-59 22.41472486709199 26.935907763979777 27.798605276545214 22.850762092383015 60-61 22.49047406991305 27.284148398948293 27.45717509921683 22.76820243192183 62-63 22.680258874931102 26.879704845538228 27.298886734821103 23.141149544709567 64-65 22.287394592800123 26.91805898403722 27.45596638419756 23.338580038965098 66-67 22.537057068365606 26.68533529562078 27.389376365323614 23.388231270689996 68-69 22.29893012648606 26.78533377321356 27.53416453275439 23.381571567545986 70-71 22.353974135492756 26.507651673061318 27.58035006087213 23.558024130573795 72-73 22.689873147125912 26.60863765529949 27.58301814589949 23.11847105167511 74-75 22.794959303172252 26.82405668007794 27.29495296320475 23.08603105354506 76-77 22.632978955874737 26.744482361123822 27.118919041971544 23.50361964102989 78-79 22.559104736841245 26.75199596422321 27.189259546956773 23.49963975197877 80-81 22.365778968035933 27.00474715766096 27.401790894525114 23.227682979777992 82-83 22.87764792127566 26.48692589470481 27.944862685009653 22.690563499009876 84-85 22.967822180388843 26.741825882963116 27.247460182919653 23.042891753728387 86-87 22.30364121616618 27.09573575756435 27.492847050249246 23.10777597602022 88-89 22.80745365240052 26.361261877636437 27.4290112905923 23.402273179370738 90-91 22.767066794470374 26.429999278630746 27.472661209938913 23.330272716959968 92-93 22.940812654747802 26.495676498774216 27.417903040191277 23.145607806286705 94-95 22.25270843429276 26.52289243540318 27.775162371578404 23.449236758725654 96-97 22.523747909540372 26.44259244850577 27.4942053083506 23.539454333603256 98-99 22.241034120973925 26.715246514003066 27.3186329631728 23.725086401850206 100 22.783078816243368 26.29546268891854 26.898676357432482 24.022782137405606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 892.0 1 634.5 2 825.0 3 1228.5 4 924.5 5 742.5 6 1070.5 7 1885.0 8 2987.0 9 3567.0 10 3280.0 11 3013.0 12 3258.0 13 3722.0 14 4308.5 15 4882.0 16 5427.0 17 5932.5 18 6369.5 19 6924.5 20 7513.0 21 8132.0 22 9330.5 23 10980.0 24 12838.5 25 15416.0 26 19148.5 27 23370.5 28 28718.0 29 37023.0 30 48191.5 31 59277.5 32 72731.0 33 87473.5 34 99775.0 35 112136.0 36 125026.0 37 142695.0 38 152218.0 39 151229.0 40 149109.0 41 145695.5 42 147142.0 43 155298.5 44 166650.0 45 184750.0 46 202181.0 47 214174.0 48 223332.0 49 224234.0 50 219536.0 51 220878.5 52 219276.0 53 209491.5 54 198292.0 55 177303.0 56 157711.5 57 135411.0 58 112762.0 59 101896.0 60 85694.0 61 65314.5 62 50804.0 63 40763.0 64 30709.0 65 20420.0 66 14410.5 67 10820.5 68 8285.0 69 6523.0 70 5268.5 71 4150.5 72 3239.0 73 2499.0 74 1912.5 75 1527.5 76 1206.0 77 831.0 78 579.5 79 419.0 80 321.0 81 255.0 82 196.5 83 139.0 84 95.5 85 73.0 86 54.5 87 47.0 88 39.5 89 27.5 90 20.0 91 16.0 92 19.0 93 13.5 94 8.5 95 8.5 96 7.0 97 4.5 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05614136175933929 2 0.12853164313838472 3 0.016729088639200997 4 0.01613367905502737 5 0.01853452415250168 6 0.05128205128205128 7 0.0561029482377797 8 0.04776721405934889 9 0.03991164890041295 10-11 0.039037741284932294 12-13 0.040199750312109865 14-15 0.019101123595505618 16-17 0.0184769038701623 18-19 0.01796792470949774 20-21 0.0212618841832325 22-23 0.025804283107653897 24-25 0.02464227408047633 26-27 0.019466052050321713 28-29 0.0184769038701623 30-31 0.016786708921540383 32-33 0.023057716316143284 34-35 0.022808028426005954 36-37 0.022894458849515027 38-39 0.021021799673485067 40-41 0.024469413233458175 42-43 0.031614328243541726 44-45 0.040363007778738115 46-47 0.046701238836070295 48-49 0.03991164890041295 50-51 0.02791702679343129 52-53 0.03979640833573418 54-55 0.03666570632862768 56-57 0.03831748775569001 58-59 0.04307116104868913 60-61 0.023701142802266397 62-63 0.026140401421300297 64-65 0.025525785076346872 66-67 0.03245942571785268 68-69 0.03943147988091809 70-71 0.04125612215499856 72-73 0.0445884951502929 74-75 0.02738884087198694 76-77 0.020752905022567943 78-79 0.020330356285412465 80-81 0.024584653798136943 82-83 0.021050609814654758 84-85 0.012724479016613848 86-87 0.014990876788629598 88-89 0.018659368097570347 90-91 0.02154038221453952 92-93 0.02025352924229329 94-95 0.023672332661096706 96-97 0.025064822817631807 98-99 0.028061077499279746 100 0.029674445404782483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5206500.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.9463447772052 #Duplication Level Percentage of deduplicated Percentage of total 1 80.4049207936111 56.240303116137966 2 12.770794864130938 17.86540841290928 3 3.4883704088202765 7.319962779778298 4 1.3321814347964127 3.7272488777624715 5 0.6587959769613282 2.3040185271186395 6 0.3837962874478723 1.6107088467624135 7 0.22527981212101628 1.1030249586972427 8 0.1495019883179871 0.8365694095814105 9 0.10473428033827613 0.6593202074280171 >10 0.45456378461490715 5.222813652215465 >50 0.018502494293816615 0.8659005898855386 >100 0.007853612838147924 0.9902123628437173 >500 4.1615486620082945E-4 0.19726609103426573 >1k 1.920712277905774E-4 0.24121968516016534 >5k 3.201187129837107E-5 0.17950972021970646 >10k+ 6.402374259674213E-5 0.6365127624654603 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15795 0.30337078651685395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12632 0.242619802170364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8017 0.1539806011716124 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.8413521559588974E-5 2 0.0 0.0 0.0 0.0 3.8413521559588974E-5 3 0.0 0.0 0.0 0.0 3.8413521559588974E-5 4 0.0 0.0 0.0 0.0 3.8413521559588974E-5 5 0.0 0.0 0.0 0.0 3.8413521559588974E-5 6 0.0 0.0 0.0 0.0 3.8413521559588974E-5 7 0.0 0.0 0.0 0.0 3.8413521559588974E-5 8 0.0 0.0 0.0 0.0 5.762028233938346E-5 9 0.0 0.0 0.0 1.9206760779794487E-5 9.603380389897244E-5 10-11 0.0 0.0 0.0 3.8413521559588974E-5 9.603380389897244E-5 12-13 0.0 0.0 0.0 7.682704311917795E-5 9.603380389897244E-5 14-15 0.0 0.0 0.0 7.682704311917795E-5 1.344473254585614E-4 16-17 0.0 0.0 0.0 8.643042350907519E-5 1.7286084701815038E-4 18-19 0.0 0.0 0.0 1.344473254585614E-4 1.9206760779794487E-4 20-21 0.0 0.0 0.0 1.536540862383559E-4 1.9206760779794487E-4 22-23 0.0 0.0 0.0 3.1691155286660906E-4 2.0167098818784211E-4 24-25 0.0 0.0 0.0 6.530298665130127E-4 2.1127436857773936E-4 26-27 0.0 0.0 0.0 0.0015557476231633535 2.1127436857773936E-4 28-29 0.0 0.0 0.0 0.003812542014789206 2.1127436857773936E-4 30-31 0.0 0.0 0.0 0.014395467204455968 2.1127436857773936E-4 32-33 0.0 0.0 0.0 0.04073753961394411 2.3048112935753384E-4 34-35 0.0 0.0 0.0 0.0783923941227312 2.4968789013732833E-4 36-37 0.0 0.0 0.0 0.14083357341784308 2.4968789013732833E-4 38-39 0.0 0.0 0.0 0.23132622683184478 2.5929127052722555E-4 40-41 0.0 0.0 0.0 0.32498799577451265 2.688946509171228E-4 42-43 0.0 1.9206760779794487E-5 0.0 0.41827523288197443 2.688946509171228E-4 44-45 0.0 1.9206760779794487E-5 0.0 0.5276961490444636 2.784980313070201E-4 46-47 0.0 1.9206760779794487E-5 0.0 0.6612311533659848 2.881014116969173E-4 48-49 0.0 1.9206760779794487E-5 0.0 0.8048785172380678 2.881014116969173E-4 50-51 0.0 1.9206760779794487E-5 0.0 0.9511860174781523 2.881014116969173E-4 52-53 0.0 1.9206760779794487E-5 0.0 1.0956592720637666 3.1691155286660906E-4 54-55 0.0 1.9206760779794487E-5 0.0 1.254403149908768 3.5532507442619804E-4 56-57 0.0 1.9206760779794487E-5 0.0 1.4362239508306924 3.6492845481609525E-4 58-59 0.0 1.9206760779794487E-5 0.0 1.6419187554019015 3.6492845481609525E-4 60-61 0.0 1.9206760779794487E-5 0.0 1.8524152501680593 3.8413521559588974E-4 62-63 0.0 1.9206760779794487E-5 0.0 2.0649956784788244 4.0334197637568423E-4 64-65 0.0 1.9206760779794487E-5 0.0 2.2813406319024296 4.225487371554787E-4 66-67 0.0 1.9206760779794487E-5 0.0 2.5244598098530684 4.225487371554787E-4 68-69 0.0 1.9206760779794487E-5 0.0 2.7939210602131954 4.32152117545376E-4 70-71 0.0 1.9206760779794487E-5 0.0 3.0746662825314512 4.417554979352732E-4 72-73 0.0 1.9206760779794487E-5 0.0 3.355344281186978 4.417554979352732E-4 74-75 0.0 1.9206760779794487E-5 0.0 3.635263612791703 4.417554979352732E-4 76-77 0.0 1.9206760779794487E-5 0.0 3.948487467588591 4.417554979352732E-4 78-79 0.0 1.9206760779794487E-5 0.0 4.2889945260731785 4.417554979352732E-4 80-81 0.0 1.9206760779794487E-5 0.0 4.6451166810717375 4.417554979352732E-4 82-83 0.0 1.9206760779794487E-5 0.0 5.008230096994142 4.513588783251704E-4 84-85 0.0 1.9206760779794487E-5 0.0 5.368462498799577 4.609622587150677E-4 86-87 0.0 1.9206760779794487E-5 0.0 5.7494285988668015 4.609622587150677E-4 88 0.0 3.8413521559588974E-5 0.0 6.053798136944204 4.609622587150677E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8295 0.0 47.030895 1 GTATCAA 14460 0.0 39.769306 1 CAACGCA 17010 0.0 32.445755 5 TACCTGG 3730 0.0 32.297733 2 ATCAACG 17055 0.0 32.222324 3 GTACTAG 3305 0.0 32.03732 1 TATCAAC 18205 0.0 31.070963 2 AACGCAG 18995 0.0 29.8471 6 TCAACGC 19295 0.0 28.846746 4 GTACCTG 4420 0.0 28.32073 1 GTACATG 19130 0.0 28.043676 1 GCATCGT 3315 0.0 27.934662 8 TAGTACT 4505 0.0 27.65292 4 CTGTGCG 4450 0.0 26.936483 9 TACATGG 19580 0.0 26.870144 2 ACCTGGG 4435 0.0 26.60567 3 ACGCAGA 20885 0.0 26.448284 7 ACATGGG 19360 0.0 26.22482 3 CGCAGAG 21340 0.0 25.838322 8 CTAGTAC 4970 0.0 25.633446 3 >>END_MODULE