FastQCFastQC Report
Mon 6 Jun 2016
ERR1211183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5206500
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143370.2753673292999136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124100.23835590127724962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80070.15378853356381444No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86100.047.2401431
GTATCAA146500.038.4518741
GTACTAG34200.034.6475641
CAACGCA173450.031.0434955
ATCAACG174000.030.903533
TACCTGG35550.030.6720052
TATCAAC188200.029.5682682
AACGCAG194950.028.5116126
TCAACGC196050.027.5955964
TAGGCAT44800.026.743765
GCATCGT40850.026.4540318
TAGTACT46950.025.929584
GTACATG190650.025.8724161
CTGTGCG46300.025.5726439
ACGCAGA213800.025.3385567
CTAGGCA49600.025.3023554
GTACCTG44950.025.2107411
TACATGG194100.025.0858022
CGCAGAG218750.024.679038
GGGCACC42150.024.20180594
Produced by FastQC (version 0.11.5)