FastQCFastQC Report
Mon 6 Jun 2016
ERR1211182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4477188
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176270.39370694284001473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139720.3120708802042711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91230.20376629259258264No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63160.14107068990625365No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78050.049.6824761
TACCTGG38950.041.5627942
GTATCAA140000.040.2696341
GTACTAG23750.035.0718421
ACCTGGG45600.034.6371883
GTACCTG48600.033.5033871
ATCAACG169200.032.533053
CAACGCA170500.032.2574235
TATCAAC179900.031.0246772
GTACATG176150.030.856611
TACATGG176900.029.8748462
AACGCAG188400.029.6916476
GCATCGT25500.029.4929968
TCAACGC187350.029.45664
ACATGGG178250.028.6924463
CATGGGG134550.027.9145934
GTGGTAT34250.027.3427011
ACGCAGA202500.027.1831687
CCTGGGG45350.026.4320664
TAACGCA5700.026.3902384
Produced by FastQC (version 0.11.5)