FastQCFastQC Report
Mon 6 Jun 2016
ERR1211182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4477188
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159030.3552006303956859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135630.3029356819503671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90830.20287287467044046No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53640.11980734335926926No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81700.050.298371
TACCTGG42350.044.3921472
GTATCAA139250.038.9312821
ACCTGGG48400.038.2634743
GTACCTG51600.036.0836141
ATCAACG173150.030.7024943
CAACGCA174400.030.497125
CCTGGGG46700.029.4906444
TATCAAC183500.029.4295432
AACGCAG192500.028.4106456
GTACTAG25800.028.0650331
TCAACGC190300.028.0343594
GTGGTAT34550.027.8978271
CTGGGGG33250.026.7072435
GTACATG176350.026.4218241
TACATGG176100.025.9154912
ACGCAGA210800.025.4984827
TGGGGGG104000.025.2544166
CGCAGAG216700.024.8042438
TGGTATC38150.024.6396792
Produced by FastQC (version 0.11.5)