##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211182_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4477188 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.998276596828187 33.0 31.0 34.0 30.0 34.0 2 32.13709028970863 33.0 31.0 34.0 30.0 34.0 3 32.2088770898162 34.0 31.0 34.0 30.0 34.0 4 35.87172350144778 37.0 35.0 37.0 35.0 37.0 5 35.74940543037282 37.0 35.0 37.0 35.0 37.0 6 35.7792853460699 37.0 35.0 37.0 33.0 37.0 7 35.716479182915705 37.0 35.0 37.0 33.0 37.0 8 35.70076418501971 37.0 35.0 37.0 33.0 37.0 9 37.27634756458742 39.0 37.0 39.0 34.0 39.0 10-11 37.242029036975886 39.0 37.0 39.0 34.0 39.0 12-13 37.1339304938725 39.0 37.0 39.0 33.0 39.0 14-15 38.30129469658187 40.0 38.0 41.0 33.0 41.0 16-17 38.246033447780164 40.0 38.0 41.0 33.0 41.0 18-19 38.27318564688372 40.0 38.0 41.0 33.5 41.0 20-21 38.23248666350396 40.0 38.0 41.0 33.5 41.0 22-23 38.0927438159845 40.0 37.0 41.0 33.0 41.0 24-25 38.01875563411677 40.0 37.0 41.0 33.0 41.0 26-27 37.86231246041042 40.0 37.0 41.0 33.0 41.0 28-29 37.82427954778758 40.0 37.0 41.0 33.0 41.0 30-31 37.82133082193556 40.0 37.0 41.0 33.0 41.0 32-33 37.661452791350285 40.0 36.5 41.0 33.0 41.0 34-35 37.49440675709843 40.0 36.0 41.0 32.0 41.0 36-37 37.330243447449604 39.5 36.0 41.0 32.0 41.0 38-39 37.23321446407879 39.0 36.0 41.0 31.0 41.0 40-41 37.12448181760516 39.0 36.0 41.0 31.0 41.0 42-43 36.97351250383053 39.0 35.0 41.0 31.0 41.0 44-45 36.82547427090397 39.0 35.0 41.0 30.5 41.0 46-47 36.575703879309955 39.0 35.0 40.5 30.0 41.0 48-49 36.33407799717144 39.0 35.0 40.0 29.5 41.0 50-51 36.1324822634207 39.0 35.0 40.0 29.0 41.0 52-53 35.92662816482131 38.0 35.0 40.0 28.5 41.0 54-55 35.681321065811844 38.0 34.5 40.0 28.0 41.0 56-57 35.42131735366038 38.0 34.0 40.0 27.5 41.0 58-59 35.14810803120173 37.5 34.0 40.0 27.0 41.0 60-61 34.878185593278644 37.0 34.0 40.0 26.5 41.0 62-63 34.563663509327725 36.5 33.0 39.5 26.0 41.0 64-65 34.1869350806801 36.0 33.0 39.0 26.0 40.5 66-67 33.87747822963878 35.5 33.0 39.0 26.0 40.0 68-69 33.54512475241156 35.0 32.0 38.0 25.0 40.0 70-71 33.21286798767441 35.0 32.0 38.0 25.0 40.0 72-73 32.86494368786837 35.0 32.0 37.0 24.5 39.5 74-75 32.528689101284115 35.0 31.0 36.5 24.0 39.0 76-77 31.578392508869406 34.0 30.5 35.5 23.0 38.0 78-79 32.098996624667095 34.0 31.0 36.0 24.0 38.0 80-81 32.04041699834807 34.0 31.0 35.0 24.0 37.0 82-83 31.789612363831942 34.0 31.0 35.0 24.0 37.0 84-85 31.535658766171984 34.0 31.0 35.0 23.5 36.0 86-87 31.293611749160412 34.0 31.0 35.0 23.0 36.0 88-89 31.0774825627157 34.0 31.0 35.0 22.0 36.0 90-91 30.85229847395285 34.0 30.0 35.0 20.0 35.0 92-93 30.66238954004165 34.0 30.0 35.0 20.0 35.0 94-95 30.467587579525365 34.0 30.0 35.0 20.0 35.0 96-97 30.28260528706858 34.0 29.0 35.0 19.0 35.0 98-99 30.09316606762995 34.0 29.0 35.0 19.0 35.0 100 28.315787945469342 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4287719415506537 1101 2 -0.45420911575717327 1101 3 -0.448710269968128 1101 4 -0.3108429193026012 1101 5 -0.3449617859182226 1101 6 -0.3672929203995352 1101 7 -0.38190097449346183 1101 8 -0.39372322488009104 1101 9 -0.6596199794558615 1101 10-11 -0.5575295580436475 1101 12-13 -0.5519654535418539 1101 14-15 -0.9246974066694236 1101 16-17 -0.7932761821834262 1101 18-19 -0.8980834659573134 1101 20-21 -0.838250310101408 1101 22-23 -0.9869887130190094 1101 24-25 -1.0028809869788802 1101 26-27 -1.082756178269328 1101 28-29 -1.1448976499534638 1101 30-31 -0.8780155045772489 1101 32-33 -0.8931534138593591 1101 34-35 -0.9696980816161442 1101 36-37 -0.8716406031894692 1101 38-39 -0.9788540446861091 1101 40-41 -0.9229765275567701 1101 42-43 -0.9829296360258297 1101 44-45 -1.0314117660066557 1101 46-47 -1.0536143809857634 1101 48-49 -0.9302668912444219 1101 50-51 -1.013537891837764 1101 52-53 -1.0727131510043577 1101 54-55 -0.9261146658955965 1101 56-57 -0.9793866752968015 1101 58-59 -1.0344893971771612 1101 60-61 -1.0674347771590433 1101 62-63 -1.267254493073331 1101 64-65 -1.098547261461718 1101 66-67 -1.1326683596169786 1101 68-69 -1.386464238927239 1101 70-71 -1.373219262770668 1101 72-73 -1.3706292984917887 1101 74-75 -1.2177388166106695 1101 76-77 -1.2178893078606556 1101 78-79 -1.0495493706225218 1101 80-81 -1.0179435914288746 1101 82-83 -1.083932754561534 1101 84-85 -0.9482403569082685 1101 86-87 -0.9589401261026609 1101 88-89 -1.1326882102558748 1101 90-91 -1.1886434241312287 1101 92-93 -1.215573054743917 1101 94-95 -1.201617581606179 1101 96-97 -1.2218277831835138 1101 98-99 -1.4356026224876963 1101 100 -1.2727382092244781 1102 1 -0.377042638546051 1102 2 -0.35656056278526904 1102 3 -0.40241843253220466 1102 4 -0.26342157864446847 1102 5 -0.349630961817482 1102 6 -0.37177259218501746 1102 7 -0.3887299102490047 1102 8 -0.4564140475425802 1102 9 -0.5905612315295343 1102 10-11 -0.5997477908343143 1102 12-13 -0.5314099441546105 1102 14-15 -0.6853093160031705 1102 16-17 -0.8228398329095512 1102 18-19 -0.7478147755408031 1102 20-21 -0.8028483549986518 1102 22-23 -0.7972104328321308 1102 24-25 -0.8735447962243654 1102 26-27 -0.9030441804942129 1102 28-29 -0.8084569444698246 1102 30-31 -0.8117567578983937 1102 32-33 -0.7263156798295753 1102 34-35 -0.8149602678038477 1102 36-37 -0.8695153319811979 1102 38-39 -0.9428373477776759 1102 40-41 -0.8930023151591655 1102 42-43 -0.8423259305874993 1102 44-45 -0.8586509735822716 1102 46-47 -1.0050636288305412 1102 48-49 -0.9844884576931392 1102 50-51 -0.9129387249587566 1102 52-53 -1.0327957513163355 1102 54-55 -1.0601923119499688 1102 56-57 -1.0662879785090738 1102 58-59 -1.135700554108027 1102 60-61 -1.1480785921612053 1102 62-63 -1.0045815201206807 1102 64-65 -0.926798288920736 1102 66-67 -1.1582619633669964 1102 68-69 -1.1999007410151776 1102 70-71 -1.2205121515600048 1102 72-73 -1.2062483302816993 1102 74-75 -1.255020586480029 1102 76-77 -1.0939845288163994 1102 78-79 -0.8837571195979983 1102 80-81 -0.9441084409290355 1102 82-83 -0.9080781653057386 1102 84-85 -0.9477661379981761 1102 86-87 -0.9780627972704607 1102 88-89 -0.9840466318792274 1102 90-91 -1.0435284017383708 1102 92-93 -1.0456499625944708 1102 94-95 -1.1512160915498235 1102 96-97 -1.3840143194390642 1102 98-99 -1.3568998029123094 1102 100 -1.2452949682748589 1103 1 -0.4031137453738971 1103 2 -0.42611424208523374 1103 3 -0.48665050727231574 1103 4 -0.2914341211570246 1103 5 -0.3310819618208214 1103 6 -0.43230518440851995 1103 7 -0.414700574955404 1103 8 -0.3778646042403082 1103 9 -0.6237647006085112 1103 10-11 -0.610875581572202 1103 12-13 -0.64934639295479 1103 14-15 -0.7921864435285357 1103 16-17 -0.7818487290816094 1103 18-19 -0.8341507301463054 1103 20-21 -0.7787877909499556 1103 22-23 -0.7231950647017342 1103 24-25 -0.9419151228039624 1103 26-27 -0.8453299492605382 1103 28-29 -0.913866183869736 1103 30-31 -0.8454938637017761 1103 32-33 -0.8026827842453557 1103 34-35 -0.891003516213793 1103 36-37 -0.9327024746609354 1103 38-39 -0.8415401626772265 1103 40-41 -0.9359065256212418 1103 42-43 -0.812695990576465 1103 44-45 -0.9807334836879633 1103 46-47 -0.9844700183219004 1103 48-49 -1.042227738730375 1103 50-51 -1.1008560357090076 1103 52-53 -1.0973613011868437 1103 54-55 -1.1740167064506792 1103 56-57 -1.1901851267483465 1103 58-59 -1.1538908409176472 1103 60-61 -1.0984123314531757 1103 62-63 -1.0883204325462614 1103 64-65 -1.1206947600562245 1103 66-67 -1.2252351485390918 1103 68-69 -1.2005557098790334 1103 70-71 -1.285540014532419 1103 72-73 -1.140896854210638 1103 74-75 -1.14772404933451 1103 76-77 -1.057258176817772 1103 78-79 -0.9694352511802116 1103 80-81 -0.9365705147673573 1103 82-83 -1.0995976681718922 1103 84-85 -1.0703616073540694 1103 86-87 -1.057487738798148 1103 88-89 -1.0702638352341403 1103 90-91 -1.054562157031853 1103 92-93 -1.1750740691287014 1103 94-95 -1.0561377251370878 1103 96-97 -1.1600337022179765 1103 98-99 -1.3803621472885048 1103 100 -1.433063255466454 1104 1 -0.4642401119220061 1104 2 -0.38874068406549256 1104 3 -0.47147889757503236 1104 4 -0.33919192372842133 1104 5 -0.4506071552282407 1104 6 -0.5251361499586906 1104 7 -0.4729122804111725 1104 8 -0.42120568359678856 1104 9 -0.6554244128239901 1104 10-11 -0.6545961498401667 1104 12-13 -0.687102978678304 1104 14-15 -0.797533558698845 1104 16-17 -0.7126081911603421 1104 18-19 -0.8255721388463968 1104 20-21 -0.8795693363343489 1104 22-23 -0.816736737706151 1104 24-25 -0.7747942593883153 1104 26-27 -0.8797768639613608 1104 28-29 -0.9790646138876298 1104 30-31 -0.8577524575347155 1104 32-33 -0.8164186376127063 1104 34-35 -0.8531798486467039 1104 36-37 -0.8091734792247394 1104 38-39 -0.7906429236181509 1104 40-41 -0.8139227096980406 1104 42-43 -0.7977510498388227 1104 44-45 -0.8582241881497623 1104 46-47 -0.9030584251141391 1104 48-49 -1.0154685398881185 1104 50-51 -0.9085649222373249 1104 52-53 -0.8955089296682885 1104 54-55 -0.9909176059868443 1104 56-57 -0.8785015245806207 1104 58-59 -0.8907868482286716 1104 60-61 -0.9231906136985586 1104 62-63 -1.0237488033545787 1104 64-65 -0.9993236829161418 1104 66-67 -1.107293968233897 1104 68-69 -1.0897540430457724 1104 70-71 -1.0374465854488832 1104 72-73 -1.0819451376169908 1104 74-75 -1.0640816511260098 1104 76-77 -0.9827853792658736 1104 78-79 -0.7045473657079491 1104 80-81 -0.739551481652331 1104 82-83 -0.805472001311319 1104 84-85 -0.7974114838378021 1104 86-87 -0.9197559556627937 1104 88-89 -0.9809763182929494 1104 90-91 -0.9340473434610175 1104 92-93 -0.9795479407351131 1104 94-95 -0.7802420226588787 1104 96-97 -0.9331716611046623 1104 98-99 -1.0680573318613575 1104 100 -1.0787041773156432 1105 1 -0.4980930675060833 1105 2 -0.46740802041238894 1105 3 -0.5163509452463408 1105 4 -0.3232081448717139 1105 5 -0.382891694916097 1105 6 -0.5341462013628515 1105 7 -0.4811712978414562 1105 8 -0.5436183926393312 1105 9 -0.6787898039978515 1105 10-11 -0.5980929967624249 1105 12-13 -0.6095923909098957 1105 14-15 -0.900828571441366 1105 16-17 -0.8384667150115064 1105 18-19 -0.7712222557304003 1105 20-21 -0.8777313121390478 1105 22-23 -0.8068520715231458 1105 24-25 -0.8605174210230757 1105 26-27 -0.9055794571120259 1105 28-29 -0.9140931063010242 1105 30-31 -0.8232125836578277 1105 32-33 -0.8140839959226227 1105 34-35 -0.8550504267419541 1105 36-37 -0.7599372867288778 1105 38-39 -0.8355705919528233 1105 40-41 -0.8776984040262192 1105 42-43 -0.8500095464067101 1105 44-45 -0.8407478284818737 1105 46-47 -0.7400937879506131 1105 48-49 -0.9216742433313883 1105 50-51 -0.8382439097800329 1105 52-53 -0.8606686918077955 1105 54-55 -0.9151905378840866 1105 56-57 -0.820408997095825 1105 58-59 -0.9148095181445299 1105 60-61 -0.9630083118587933 1105 62-63 -0.966729686464376 1105 64-65 -0.9366324365048229 1105 66-67 -0.9572034373883085 1105 68-69 -0.9312790537420526 1105 70-71 -0.9819109342778773 1105 72-73 -0.9701060592954107 1105 74-75 -0.9852836031198571 1105 76-77 -0.9082037123821785 1105 78-79 -0.913742199249004 1105 80-81 -0.8815936343022202 1105 82-83 -0.853611158382332 1105 84-85 -0.8982919893223595 1105 86-87 -0.9414412555617204 1105 88-89 -0.8357771194289221 1105 90-91 -0.9017930013908355 1105 92-93 -0.8843957435018801 1105 94-95 -0.9546212972959829 1105 96-97 -0.8751868405231527 1105 98-99 -0.9375931442516823 1105 100 -1.0369091862757145 1106 1 -0.43549510722696283 1106 2 -0.5081119541598014 1106 3 -0.5027630302091524 1106 4 -0.3528653797463406 1106 5 -0.31808378141252547 1106 6 -0.44352891741223033 1106 7 -0.4271662368798772 1106 8 -0.4580605366466983 1106 9 -0.659936955289929 1106 10-11 -0.6282862334774038 1106 12-13 -0.7094743018062957 1106 14-15 -0.7823721647240944 1106 16-17 -0.7606782018606708 1106 18-19 -0.7761237203420137 1106 20-21 -0.8027915069093865 1106 22-23 -0.7494699689236484 1106 24-25 -0.9400872392885447 1106 26-27 -0.8320275822557974 1106 28-29 -0.9276112414133664 1106 30-31 -0.7976570281022717 1106 32-33 -0.8408940565008081 1106 34-35 -0.8976966961998158 1106 36-37 -0.8792356534185473 1106 38-39 -0.8756288696922567 1106 40-41 -0.8950298970098842 1106 42-43 -0.8088501261168517 1106 44-45 -1.0035328909770769 1106 46-47 -0.989288634971544 1106 48-49 -1.0379958444355992 1106 50-51 -1.0963383810629068 1106 52-53 -1.1302607476317306 1106 54-55 -0.9624599190665108 1106 56-57 -0.9781039591386147 1106 58-59 -1.0033610095945704 1106 60-61 -0.9210322364044643 1106 62-63 -0.9942260441056661 1106 64-65 -1.0272313169587761 1106 66-67 -1.1440211660900914 1106 68-69 -1.0627375308527078 1106 70-71 -1.065985008351948 1106 72-73 -1.004700798962613 1106 74-75 -1.0907141682605683 1106 76-77 -1.105502232434322 1106 78-79 -0.8253923794499123 1106 80-81 -0.7927507541549907 1106 82-83 -0.8643135585050246 1106 84-85 -0.920927523407169 1106 86-87 -0.8758611620106116 1106 88-89 -0.8204960826986145 1106 90-91 -0.9629746592391584 1106 92-93 -0.9263756530180238 1106 94-95 -1.0887007390565806 1106 96-97 -1.0086098909027257 1106 98-99 -0.9872833520578297 1106 100 -0.8665280805322908 1107 1 -0.4596733026140889 1107 2 -0.4491643356566293 1107 3 -0.45669950481874366 1107 4 -0.2689862059832677 1107 5 -0.4005278058058508 1107 6 -0.3986424722771602 1107 7 -0.4214654337177137 1107 8 -0.42686256437502124 1107 9 -0.596919387880881 1107 10-11 -0.6145482255987176 1107 12-13 -0.590679802331195 1107 14-15 -0.792596928371097 1107 16-17 -0.7711526896944463 1107 18-19 -0.7830522672703495 1107 20-21 -0.7968386631748317 1107 22-23 -0.8256765905197838 1107 24-25 -0.8169007539136999 1107 26-27 -0.8168790737260068 1107 28-29 -0.8947806924448187 1107 30-31 -0.8504182042916142 1107 32-33 -0.7328590790781391 1107 34-35 -0.8019439079991315 1107 36-37 -0.7385612585371675 1107 38-39 -0.8374812060492687 1107 40-41 -0.8159199533307273 1107 42-43 -0.7730854887128658 1107 44-45 -0.8253912558761201 1107 46-47 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1.1952716061196114 2310 30-31 1.018911914587349 2310 32-33 1.1799449873510426 2310 34-35 1.2777740647314175 2310 36-37 1.0724676337959593 2310 38-39 1.1745006052768119 2310 40-41 1.2748244157483484 2310 42-43 1.3658961341935054 2310 44-45 1.3994762478105471 2310 46-47 1.1229775470499845 2310 48-49 1.3363664434685134 2310 50-51 1.439497837389247 2310 52-53 1.4241440681181317 2310 54-55 1.4959855824389976 2310 56-57 1.4947934911878065 2310 58-59 1.491199443973123 2310 60-61 1.5621800257127418 2310 62-63 1.0406037916452178 2310 64-65 1.1696170877183505 2310 66-67 1.4488828866256398 2310 68-69 1.4977801340631132 2310 70-71 1.4982744960929182 2310 72-73 1.5375891493108398 2310 74-75 1.507512890492471 2310 76-77 1.50933390025725 2310 78-79 1.450864371258195 2310 80-81 1.2818598399778125 2310 82-83 1.3057831864700553 2310 84-85 1.3188150773938112 2310 86-87 1.3361916759007286 2310 88-89 1.3179952595953246 2310 90-91 1.3902129437763193 2310 92-93 1.4786254101781218 2310 94-95 1.5451591798585618 2310 96-97 1.5378554224699315 2310 98-99 1.578512911365756 2310 100 1.739760025651048 2311 1 0.46398061817524905 2311 2 0.35466116559694427 2311 3 0.4982352862309938 2311 4 0.3432928391355077 2311 5 0.46819824405414323 2311 6 0.5363981879014048 2311 7 0.5408295405184802 2311 8 0.5641372893806889 2311 9 0.7625483984542356 2311 10-11 0.7882511855169199 2311 12-13 0.8003306173339766 2311 14-15 1.0134256079250292 2311 16-17 0.803199395116792 2311 18-19 0.965766990053524 2311 20-21 0.8695746631885442 2311 22-23 0.9733863637277622 2311 24-25 1.061736843600265 2311 26-27 0.479789101548306 2311 28-29 0.7815132955481019 2311 30-31 0.9762351644931613 2311 32-33 1.0904769426615886 2311 34-35 1.1450024088802877 2311 36-37 1.1968944942176236 2311 38-39 1.1702364479024325 2311 40-41 1.1603796202862142 2311 42-43 1.2099380205732757 2311 44-45 1.178930563382714 2311 46-47 0.862022218329983 2311 48-49 1.1048758344655667 2311 50-51 1.2097674246569028 2311 52-53 1.2037021711582483 2311 54-55 1.225419617638785 2311 56-57 1.342809452506664 2311 58-59 1.3602196231522825 2311 60-61 1.404299131782814 2311 62-63 1.40662931868183 2311 64-65 1.4518513871743508 2311 66-67 1.453192107462769 2311 68-69 1.4999735128471698 2311 70-71 1.51309805322488 2311 72-73 1.5654248901590648 2311 74-75 1.560171088735494 2311 76-77 1.4770114785104802 2311 78-79 1.2067171698969794 2311 80-81 1.1811212309562933 2311 82-83 1.246175817672306 2311 84-85 1.2294703948406855 2311 86-87 1.3184871049235767 2311 88-89 1.342894056382736 2311 90-91 1.3126242555617509 2311 92-93 1.438401915721382 2311 94-95 1.4991398601143864 2311 96-97 1.573578212655864 2311 98-99 1.5823845231657216 2311 100 1.7245507441451444 2312 1 0.42073776105875993 2312 2 0.4686531001309078 2312 3 0.5549194649487958 2312 4 0.29460233113910306 2312 5 0.44395103915923784 2312 6 0.5288762891525707 2312 7 0.4815351097545957 2312 8 0.5477580772091244 2312 9 0.7579860663829976 2312 10-11 0.8052893839204671 2312 12-13 0.8919725129866478 2312 14-15 1.0677352215536615 2312 16-17 1.0483014000192057 2312 18-19 0.971944861584987 2312 20-21 1.0272147148160329 2312 22-23 1.1556405399338203 2312 24-25 1.1221984187555876 2312 26-27 1.1977677100891313 2312 28-29 1.200836361933483 2312 30-31 1.1990605723504757 2312 32-33 1.0292099318478094 2312 34-35 1.120434911454197 2312 36-37 1.2815385354430404 2312 38-39 1.0651005602406869 2312 40-41 1.2145206741351373 2312 42-43 1.3671532530650907 2312 44-45 1.0675038640708934 2312 46-47 1.1082863918896706 2312 48-49 1.3148754642709477 2312 50-51 1.1345660422206407 2312 52-53 1.3690022298212483 2312 54-55 1.2113616849669455 2312 56-57 1.3080875129966145 2312 58-59 1.3584227860328824 2312 60-61 1.3883511800609654 2312 62-63 1.4090878741635962 2312 64-65 1.4381895048479691 2312 66-67 1.4026919701867868 2312 68-69 1.4555467024045825 2312 70-71 1.3203609641060794 2312 72-73 1.3690678911122731 2312 74-75 1.4568057301296378 2312 76-77 1.410191349346217 2312 78-79 1.2710445286220455 2312 80-81 1.149597964767139 2312 82-83 1.160360000099299 2312 84-85 1.20761360500995 2312 86-87 1.2579150503763685 2312 88-89 1.243942011133445 2312 90-91 1.3481596280920698 2312 92-93 1.4635226785987712 2312 94-95 1.4184431140310245 2312 96-97 1.0850338725498503 2312 98-99 0.8975614387410431 2312 100 1.1248887973332486 2313 1 0.09903191762236219 2313 2 0.3215887673316189 2313 3 0.4190043705339832 2313 4 0.32598028002464474 2313 5 0.4483270404983415 2313 6 0.3880352041593014 2313 7 0.3753333832666357 2313 8 0.4990760586689049 2313 9 0.7086072619586403 2313 10-11 0.46014190975268576 2313 12-13 0.5502647963984941 2313 14-15 0.6350620745790039 2313 16-17 0.9331942318261213 2313 18-19 0.8857267416979653 2313 20-21 0.8221045974052927 2313 22-23 0.8064196249323956 2313 24-25 0.6849230973307101 2313 26-27 0.8750668378115094 2313 28-29 0.7424674918558125 2313 30-31 0.939574254329159 2313 32-33 1.0224728957849933 2313 34-35 1.0502910241727648 2313 36-37 1.0684044204341845 2313 38-39 1.0743162011907046 2313 40-41 1.1434169626377013 2313 42-43 1.1784275675048335 2313 44-45 0.9939889678647589 2313 46-47 1.042114981233695 2313 48-49 1.109661725699162 2313 50-51 1.0294211187275977 2313 52-53 1.086421013415098 2313 54-55 1.078775088231275 2313 56-57 0.8755332646618541 2313 58-59 0.8795751197674164 2313 60-61 0.7086063272428689 2313 62-63 1.1117665476001761 2313 64-65 1.2141349815185976 2313 66-67 1.2108622994629528 2313 68-69 1.2570680071809903 2313 70-71 1.2347785201877102 2313 72-73 1.2421970641888365 2313 74-75 1.2402308611838322 2313 76-77 1.1588632050417935 2313 78-79 1.0479597930818478 2313 80-81 0.988420230563392 2313 82-83 1.1433966846472536 2313 84-85 1.0375523489620555 2313 86-87 1.067739664918932 2313 88-89 1.0663851589848967 2313 90-91 1.0226088417668748 2313 92-93 1.1676610155057041 2313 94-95 1.3089368024395363 2313 96-97 1.3633297561328028 2313 98-99 1.3504287558297676 2313 100 1.3954688898483454 2314 1 -0.2663353254157421 2314 2 0.1317213510720805 2314 3 0.3671832884248474 2314 4 0.27175340508616586 2314 5 0.36998214003403973 2314 6 0.42155545529503513 2314 7 0.49019877362399455 2314 8 0.5539732488921487 2314 9 0.5034437403804688 2314 10-11 0.6959161334242552 2314 12-13 0.5734605605497549 2314 14-15 0.8518021994616021 2314 16-17 0.9547946999862802 2314 18-19 0.9723596215736592 2314 20-21 1.0416702439780892 2314 22-23 1.0732317577206274 2314 24-25 0.9758616175900059 2314 26-27 0.8913251106986309 2314 28-29 0.9256758764258919 2314 30-31 0.9621925683760821 2314 32-33 1.0329520447646772 2314 34-35 1.109814069693826 2314 36-37 1.0915825089288518 2314 38-39 0.9153638482344988 2314 40-41 0.9494841453610263 2314 42-43 1.1023369930895015 2314 44-45 1.099099911162881 2314 46-47 0.9747116521189554 2314 48-49 0.9832862196995009 2314 50-51 0.9293093269979522 2314 52-53 1.0944336604833538 2314 54-55 0.9953559146579636 2314 56-57 0.9876508382347353 2314 58-59 1.1035029824215812 2314 60-61 1.219874354373097 2314 62-63 1.177586681750256 2314 64-65 1.1962179842265073 2314 66-67 0.8425381910094742 2314 68-69 0.8893978300382841 2314 70-71 1.070119173599629 2314 72-73 1.1746470271243439 2314 74-75 1.2105670984009365 2314 76-77 0.9908345383899935 2314 78-79 0.8817203787606509 2314 80-81 0.9657591550241591 2314 82-83 0.9583343956334112 2314 84-85 0.9601192404449499 2314 86-87 1.0170712395697592 2314 88-89 0.9216428782160655 2314 90-91 1.0647745284770096 2314 92-93 1.011145183827992 2314 94-95 0.9303309684939016 2314 96-97 0.9625860460831852 2314 98-99 1.189089373433525 2314 100 1.1947261859514882 2315 1 0.46033257526206484 2315 2 0.49473265237048736 2315 3 0.4710406053256335 2315 4 0.34701951397980224 2315 5 0.4256030383492799 2315 6 0.5161041101107031 2315 7 0.4394267313804221 2315 8 0.342667709977043 2315 9 0.5543419209993132 2315 10-11 0.6520089400367581 2315 12-13 0.4780925111175165 2315 14-15 0.6936532498294454 2315 16-17 0.8333624842183127 2315 18-19 0.8613997852708479 2315 20-21 0.6576939908397677 2315 22-23 0.9440789512427372 2315 24-25 0.9160948793506947 2315 26-27 0.9118347460820004 2315 28-29 0.9170972889872289 2315 30-31 0.948489760888549 2315 32-33 0.8637119039527832 2315 34-35 0.9117329009282216 2315 36-37 0.989760300766882 2315 38-39 1.005371757124088 2315 40-41 1.0312040764358912 2315 42-43 1.009378832798184 2315 44-45 1.0871885176501905 2315 46-47 1.0524784172821455 2315 48-49 0.9146356445381301 2315 50-51 0.9761586273136373 2315 52-53 0.9931018371309293 2315 54-55 0.7894917590917885 2315 56-57 0.7090303505086624 2315 58-59 0.7461090456792405 2315 60-61 0.9612288610939714 2315 62-63 1.0170646277274926 2315 64-65 1.0921726376825234 2315 66-67 1.1330196909388022 2315 68-69 1.1348201647424858 2315 70-71 1.0019966430241993 2315 72-73 1.0723993207023241 2315 74-75 0.38188142123171076 2315 76-77 0.5198855389486603 2315 78-79 0.7354230516287821 2315 80-81 0.7984641298839819 2315 82-83 0.7952550778845158 2315 84-85 0.9019923010842597 2315 86-87 0.8997967102328204 2315 88-89 0.890948444055045 2315 90-91 0.9318484076022528 2315 92-93 0.9595990463950521 2315 94-95 1.019324789463564 2315 96-97 1.123630692606305 2315 98-99 1.167576184098074 2315 100 1.1089804954549436 2316 1 0.3571456520394207 2316 2 0.16228224507433708 2316 3 0.261968185093437 2316 4 0.08163616846817945 2316 5 0.3714339360245802 2316 6 0.4718988112013349 2316 7 0.4758354212670284 2316 8 0.5191995359086832 2316 9 0.6877230879501397 2316 10-11 0.7050203267380653 2316 12-13 0.4868856763904432 2316 14-15 0.6151198880801019 2316 16-17 0.8919668859936323 2316 18-19 0.889075051163509 2316 20-21 0.8407321697794501 2316 22-23 0.8383757226427022 2316 24-25 0.517767567505274 2316 26-27 0.8170045317440895 2316 28-29 1.0102912485553048 2316 30-31 0.6474757152605619 2316 32-33 0.7262553736829673 2316 34-35 0.8220019058265962 2316 36-37 0.893567301930581 2316 38-39 0.8073445724741433 2316 40-41 0.7568644442202626 2316 42-43 0.7355029904664434 2316 44-45 0.5869613925385551 2316 46-47 0.7674115381719702 2316 48-49 0.8082237409360644 2316 50-51 0.7955706865271921 2316 52-53 0.8209052456990307 2316 54-55 0.6865552012178497 2316 56-57 0.8810425761588476 2316 58-59 0.7974267705040035 2316 60-61 0.9120478172791948 2316 62-63 0.9867989380303257 2316 64-65 1.043717995047679 2316 66-67 0.9555061933338678 2316 68-69 0.9276950531081596 2316 70-71 0.9310614518250446 2316 72-73 0.9286108914311271 2316 74-75 0.9194134907291485 2316 76-77 0.8339560986087555 2316 78-79 0.5049714738636375 2316 80-81 0.4577188861828674 2316 82-83 0.4906072672704589 2316 84-85 0.6422568744381572 2316 86-87 0.6793045381671128 2316 88-89 0.614031276725342 2316 90-91 0.560301257322152 2316 92-93 0.4268033169154677 2316 94-95 0.5487359353053556 2316 96-97 0.7127163059861878 2316 98-99 0.8553031738953756 2316 100 0.46511774478513956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 6.0 12 23.0 13 136.0 14 563.0 15 1562.0 16 3845.0 17 7731.0 18 12713.0 19 17540.0 20 23866.0 21 31313.0 22 37768.0 23 39260.0 24 39751.0 25 43645.0 26 51328.0 27 62543.0 28 77150.0 29 94768.0 30 116571.0 31 145235.0 32 183319.0 33 240914.0 34 336621.0 35 461082.0 36 680950.0 37 952251.0 38 724838.0 39 89892.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.735757778895035 20.070655270655273 13.49806156080666 23.695525389643038 2 15.812849493923418 20.432579556632422 40.41195053681016 23.342620412634002 3 18.784223490280063 26.34769860010346 30.02154477319246 24.846533136424025 4 12.59688355027354 16.611152494763985 38.13249402220954 32.65946993275293 5 12.85570317797689 37.67293220655465 34.39466915394217 15.076695461526295 6 30.19086980488646 36.833543733254 18.51117263782535 14.464413824034192 7 27.195092778743295 32.33634648059025 22.594248984344308 17.874311756322147 8 26.383278968852768 35.30577675094278 20.125690500376574 18.185253779827875 9 26.833398558960393 15.801801656909628 21.018131830820806 36.34666795330917 10-11 25.657569402099988 26.02742357337336 27.750096601201207 20.56491042332545 12-13 25.629491100217365 23.850629010888085 28.130201367465475 22.389678521429076 14-15 23.009723310146857 25.16523239298675 26.177990649915937 25.647053646950457 16-17 22.014578839241004 27.613132748470353 27.235506147404593 23.136782264884054 18-19 21.638805428764663 27.00475164321891 29.525977019504207 21.830465908512217 20-21 22.74740305745481 26.47306747002806 29.08606920236541 21.693460270151714 22-23 22.71389988537448 26.23437970440375 28.88546337567241 22.166257034549364 24-25 22.222270615952418 26.10217092784629 29.47841535400535 22.197143102195945 26-27 22.144276088936618 26.39372763931469 28.95088098140654 22.511115290342147 28-29 21.842724867296475 26.885531393146067 29.166945394974668 22.10479834458279 30-31 22.728462629663575 26.41193442045875 29.14643671850343 21.713166231374245 32-33 22.701734155438892 26.539610537134926 28.72215469634241 22.03650061108377 34-35 22.658957135561117 26.61097430796748 28.992544265907778 21.73752429056362 36-37 22.548973389773174 26.470006019038394 28.800316725643114 22.18070386554532 38-39 22.49362318630126 26.674422540214277 28.90981199918904 21.92214227429542 40-41 22.403340538482027 26.052347238656075 29.319032481242136 22.225279741619758 42-43 22.332813773295747 26.377062107433343 28.685342610888025 22.60478150838289 44-45 22.087797577497195 26.918250302952462 28.76910275812387 22.224849361426475 46-47 22.168809701643827 26.565770392955084 28.287156969479472 22.978262935921617 48-49 22.563292567051725 26.572972120580758 28.308941614887967 22.554793697479557 50-51 22.438969636015273 26.70625624404216 28.1599725694935 22.694801550449068 52-53 22.21811834750678 27.107046798393224 27.820402479539506 22.854432374560485 54-55 22.184094577011354 26.900659514941 28.031663418420628 22.88358248962702 56-57 22.649441070478712 26.746668015731796 27.7078769210358 22.896013992753687 58-59 22.091318070841254 26.98328258990299 28.21435990680781 22.71103943244795 60-61 22.442529807528437 27.319642088527225 27.75446713661217 22.48336096733217 62-63 22.542137694006495 26.986953159245164 27.61696508092503 22.85394406582331 64-65 22.077194635030622 26.81456926046953 27.773249040353896 23.334987064145952 66-67 22.171395087519812 26.71277312787341 27.730641811951966 23.385189972654807 68-69 21.847490635900595 26.766758975160066 27.920475467517768 23.46527492142157 70-71 21.940536645046677 26.51756135440584 28.0220110502176 23.519890950329884 72-73 22.025268117489162 26.804408664535647 27.862589151376433 23.307734066598762 74-75 22.265550989693743 26.90097566250675 27.581412454018654 23.252060893780854 76-77 22.016005286986115 26.70207261294722 27.59011826905719 23.691803831009477 78-79 21.79728663823578 26.799736048914053 27.7413462532952 23.66163105955497 80-81 21.60171269860592 27.028597465572368 27.76743884774784 23.602250988073873 82-83 22.144229024730517 26.557421076574244 28.210427017224937 23.0879228814703 84-85 22.221455001439654 26.912935599375782 27.501948871140762 23.363660528043802 86-87 21.470133954804904 27.13984037787093 27.833855950341867 23.556169716982303 88-89 21.766704252235296 26.4697428722452 27.85625922643759 23.90729364908191 90-91 21.952413364068892 26.36300032803267 27.926607829344352 23.757978478554083 92-93 21.879285774613333 26.600927436599026 27.787281239763857 23.732505549023788 94-95 21.209066665296604 26.479976420049255 28.105654977472728 24.20530193718141 96-97 21.24656174639439 26.50908188116712 27.939498128051067 24.30485824438742 98-99 21.00381586573997 26.720029882626466 27.756974446623513 24.519179805010054 100 21.074483938932868 26.46205200045579 27.656203569026726 24.80726049158461 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2250.0 1 1591.0 2 2544.0 3 3605.0 4 2176.0 5 1349.0 6 1868.0 7 3384.5 8 4996.0 9 5527.0 10 4633.0 11 3583.5 12 3544.5 13 3910.0 14 4344.0 15 4941.5 16 5515.5 17 6030.0 18 6675.5 19 7261.0 20 7838.5 21 8643.0 22 9729.5 23 11159.5 24 12894.5 25 15233.5 26 18654.0 27 22799.5 28 27582.5 29 34473.0 30 43810.5 31 53056.5 32 64441.5 33 77096.5 34 87916.0 35 98696.5 36 109514.5 37 124354.5 38 132267.0 39 131137.5 40 128161.0 41 124057.0 42 125251.0 43 130704.0 44 139582.5 45 154537.0 46 166928.0 47 175414.5 48 183722.5 49 187338.0 50 186475.5 51 186487.0 52 184585.0 53 176682.5 54 167261.5 55 151994.0 56 135913.5 57 117708.5 58 99660.0 59 88348.5 60 72398.0 61 55118.5 62 43085.5 63 33970.0 64 24929.5 65 16636.5 66 11644.5 67 8650.5 68 6758.0 69 5290.0 70 4201.0 71 3315.5 72 2639.5 73 2179.0 74 1736.5 75 1309.0 76 942.0 77 583.0 78 364.0 79 261.5 80 172.5 81 105.0 82 68.0 83 40.5 84 21.0 85 15.5 86 12.0 87 8.0 88 4.5 89 4.0 90 4.5 91 2.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04328609832778967 2 0.0 3 0.0 4 0.032475741469869035 5 0.0 6 0.0 7 6.923988896602064E-4 8 0.0 9 0.009380888182493118 10-11 4.020380649639908E-4 12-13 0.0 14-15 2.010190324819954E-4 16-17 3.1269627274977063E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.360507532853211E-4 26-27 4.91379857178211E-4 28-29 1.8985130845521788E-4 30-31 0.0027025892144801602 32-33 0.015969845358291858 34-35 0.0261324742226594 36-37 0.03104627279444151 38-39 0.02256997025811737 40-41 0.0014629718475078553 42-43 5.583862013388761E-5 44-45 0.009224540046118232 46-47 0.015847000393997304 48-49 0.0027249246625337155 50-51 0.009392055906519895 52-53 0.008688489292832913 54-55 0.009224540046118232 56-57 3.015285487229931E-4 58-59 0.009336217286386008 60-61 0.0048021213315143346 62-63 0.0011279401267045297 64-65 0.032375231953628035 66-67 0.056821379848244036 68-69 0.056251825922878376 70-71 0.04831157413983956 72-73 0.04164444289585338 74-75 0.03260975415819036 76-77 0.028857398885193113 78-79 0.013423604280186581 80-81 9.604242663028669E-4 82-83 0.03537934971683119 84-85 0.048278070967759226 86-87 0.05433097739027265 88-89 0.06595657810214804 90-91 0.045206946860395406 92-93 0.0363174385350805 94-95 0.011245898094964965 96-97 0.0037858584450775794 98-99 0.02252529936201026 100 0.03256508326208325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4477188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.44907971542325 #Duplication Level Percentage of deduplicated Percentage of total 1 80.04320014109199 53.988401869940915 2 13.094178234912674 17.663805431491703 3 3.4694968522024228 7.020431092698338 4 1.3009812382011101 3.509999489747869 5 0.653308601212344 2.2032531960971533 6 0.3611717871415951 1.4616422797125197 7 0.22547606971553635 1.0645707380114482 8 0.16404348533949814 0.8851665695567742 9 0.11249154386152581 0.6828705998304376 >10 0.5280718625592487 6.12548242151573 >50 0.03002890817586395 1.3858296400272119 >100 0.016352693142883706 2.0534953593606664 >500 6.959511129288876E-4 0.32021763947623494 >1k 3.479755364978614E-4 0.4896494925023414 >5k 7.732789699952427E-5 0.3767557636440831 >10k+ 7.732789699952427E-5 0.7684284163865545 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15903 0.3552006303956859 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13563 0.3029356819503671 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9083 0.20287287467044046 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5364 0.11980734335926926 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2335448053555045E-5 2 0.0 0.0 0.0 0.0 2.2335448053555045E-5 3 0.0 0.0 0.0 0.0 2.2335448053555045E-5 4 0.0 0.0 0.0 0.0 2.2335448053555045E-5 5 0.0 0.0 0.0 0.0 2.2335448053555045E-5 6 0.0 0.0 0.0 0.0 4.467089610711009E-5 7 0.0 0.0 0.0 0.0 4.467089610711009E-5 8 0.0 0.0 0.0 0.0 4.467089610711009E-5 9 0.0 0.0 0.0 0.0 6.700634416066514E-5 10-11 0.0 0.0 0.0 0.0 6.700634416066514E-5 12-13 0.0 0.0 0.0 0.0 6.700634416066514E-5 14-15 0.0 0.0 0.0 0.0 1.1167724026777522E-4 16-17 0.0 0.0 0.0 0.0 1.1167724026777522E-4 18-19 0.0 0.0 0.0 0.0 1.3401268832133028E-4 20-21 0.0 0.0 0.0 2.2335448053555045E-5 1.3401268832133028E-4 22-23 0.0 0.0 0.0 1.6751586040166284E-4 1.5634813637488532E-4 24-25 0.0 0.0 0.0 8.040761299279816E-4 2.2335448053555044E-4 26-27 0.0 0.0 0.0 0.0017198295001237384 2.2335448053555044E-4 28-29 0.0 0.0 0.0 0.004087386993800573 2.34522204562328E-4 30-31 0.0 0.0 0.0 0.014116003169846788 2.456899285891055E-4 32-33 0.0 0.0 0.0 0.04037132235680074 2.6802537664266055E-4 34-35 0.0 0.0 0.0 0.07806239094717488 2.903608246962156E-4 36-37 0.0 0.0 0.0 0.13799956579888983 2.903608246962156E-4 38-39 0.0 0.0 0.0 0.23171910583160682 3.015285487229931E-4 40-41 0.0 0.0 0.0 0.34294963713831095 3.1269627274977063E-4 42-43 0.0 0.0 0.0 0.44508964108721816 3.2386399677654815E-4 44-45 0.0 0.0 0.0 0.567878766761637 3.350317208033257E-4 46-47 0.0 0.0 0.0 0.7046945538136884 3.573671688568807E-4 48-49 0.0 0.0 0.0 0.8557715244479347 4.020380649639908E-4 50-51 0.0 0.0 0.0 1.0159055192678976 4.020380649639908E-4 52-53 0.0 0.0 0.0 1.1774243118671808 4.69044409124656E-4 54-55 0.0 0.0 0.0 1.3542987250032832 5.583862013388761E-4 56-57 0.0 0.0 0.0 1.5632245060962373 5.695539253656537E-4 58-59 0.0 0.0 0.0 1.7966634414279676 5.807216493924312E-4 60-61 0.0 1.1167724026777522E-5 0.0 2.0276343097497804 5.918893734192087E-4 62-63 0.0 2.2335448053555045E-5 0.0 2.273703940955796 6.700634416066514E-4 64-65 0.0 2.2335448053555045E-5 0.0 2.5180760781097424 6.700634416066514E-4 66-67 0.0 2.2335448053555045E-5 0.0 2.789864977749427 6.700634416066514E-4 68-69 0.0 2.2335448053555045E-5 0.0 3.088735608154047 6.700634416066514E-4 70-71 0.0 2.2335448053555045E-5 0.0 3.406211666787278 6.923988896602064E-4 72-73 0.0 2.2335448053555045E-5 0.0 3.710409301552671 7.370697857673165E-4 74-75 0.0 2.2335448053555045E-5 0.0 4.018035427594285 7.370697857673165E-4 76-77 0.0 2.2335448053555045E-5 0.0 4.364257207872441 7.370697857673165E-4 78-79 0.0 2.2335448053555045E-5 0.0 4.743792308922476 7.370697857673165E-4 80-81 0.0 2.2335448053555045E-5 0.0 5.124913226784312 7.70572957847649E-4 82-83 0.0 2.2335448053555045E-5 0.0 5.5156607227572305 8.934179221422018E-4 84-85 0.0 2.2335448053555045E-5 0.0 5.9130306790780285 8.934179221422018E-4 86-87 0.0 2.2335448053555045E-5 0.0 6.328749205974821 8.934179221422018E-4 88 0.0 2.2335448053555045E-5 0.0 6.656387893472421 9.157533701957568E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8170 0.0 50.29837 1 TACCTGG 4235 0.0 44.392147 2 GTATCAA 13925 0.0 38.931282 1 ACCTGGG 4840 0.0 38.263474 3 GTACCTG 5160 0.0 36.083614 1 ATCAACG 17315 0.0 30.702494 3 CAACGCA 17440 0.0 30.49712 5 CCTGGGG 4670 0.0 29.490644 4 TATCAAC 18350 0.0 29.429543 2 AACGCAG 19250 0.0 28.410645 6 GTACTAG 2580 0.0 28.065033 1 TCAACGC 19030 0.0 28.034359 4 GTGGTAT 3455 0.0 27.897827 1 CTGGGGG 3325 0.0 26.707243 5 GTACATG 17635 0.0 26.421824 1 TACATGG 17610 0.0 25.915491 2 ACGCAGA 21080 0.0 25.498482 7 TGGGGGG 10400 0.0 25.254416 6 CGCAGAG 21670 0.0 24.804243 8 TGGTATC 3815 0.0 24.639679 2 >>END_MODULE