FastQCFastQC Report
Mon 6 Jun 2016
ERR1211181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103575
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130540.42061171391057084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100250.3230145880154338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67630.2179099908975939No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.1513415979958596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53550.050.0987551
TACCTGG27150.046.975992
GTATCAA97450.040.763561
GTACCTG34850.037.2750171
ACCTGGG34600.035.5928153
ATCAACG118450.032.976393
CAACGCA118350.032.9248245
TATCAAC124000.031.7672982
TATCACG2600.030.771742
TCAACGC127350.030.7087084
AACGCAG130400.030.6763136
GTACTAG13000.030.0501231
GTACATG102850.029.9742641
TACATGG103900.029.0801032
ACGCAGA140500.027.6681677
ACATGGG105600.027.3746383
CGCAGAG145000.026.8423488
CCTGGGG35050.026.418944
CATGGGG78500.026.4062144
GTACACG5350.026.3924061
Produced by FastQC (version 0.11.5)