##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211181_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3103575 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.546557437793513 31.0 31.0 34.0 30.0 34.0 2 31.547554030432646 31.0 31.0 34.0 29.0 34.0 3 31.61350958169208 33.0 31.0 34.0 28.0 34.0 4 35.21617586170787 37.0 35.0 37.0 32.0 37.0 5 35.27836253352988 37.0 35.0 37.0 33.0 37.0 6 35.33945788324755 37.0 35.0 37.0 33.0 37.0 7 35.27576552846315 37.0 35.0 37.0 32.0 37.0 8 35.28033767510049 37.0 35.0 37.0 32.0 37.0 9 36.86875683687361 39.0 37.0 39.0 33.0 39.0 10-11 36.81392893679063 39.0 37.0 39.0 32.5 39.0 12-13 36.71311181460091 39.0 37.0 39.0 32.0 39.0 14-15 37.92413603666739 40.0 38.0 41.0 32.5 41.0 16-17 37.89686313364426 40.0 38.0 41.0 32.5 41.0 18-19 37.913036095470545 40.0 38.0 41.0 32.0 41.0 20-21 37.87847031246224 40.0 38.0 41.0 32.0 41.0 22-23 37.760939400530035 40.0 37.0 41.0 32.0 41.0 24-25 37.670955269326505 40.0 37.0 41.0 32.0 41.0 26-27 37.52668970461484 40.0 37.0 41.0 32.0 41.0 28-29 37.391508018978115 40.0 37.0 41.0 31.0 41.0 30-31 37.21707256953674 40.0 36.0 41.0 31.0 41.0 32-33 36.999132774300605 39.0 36.0 41.0 30.5 41.0 34-35 36.81960867709013 39.0 35.5 41.0 30.0 41.0 36-37 36.68565766897852 39.0 35.0 41.0 30.0 41.0 38-39 36.540156754710296 39.0 35.0 41.0 30.0 41.0 40-41 36.37237266700498 39.0 35.0 41.0 29.5 41.0 42-43 36.163507406780894 39.0 35.0 40.0 28.5 41.0 44-45 35.953582884254445 39.0 35.0 40.0 27.5 41.0 46-47 35.71743731019872 38.0 35.0 40.0 27.0 41.0 48-49 35.50491932690527 38.0 34.5 40.0 26.0 41.0 50-51 34.83006661672427 37.5 33.5 39.5 25.0 40.5 52-53 34.942236453122604 38.0 33.5 40.0 25.5 40.5 54-55 35.43059439517331 38.0 34.0 40.0 26.0 41.0 56-57 35.405985838911576 38.0 34.0 40.0 26.0 41.0 58-59 35.215679015329094 38.0 34.0 40.0 26.0 41.0 60-61 34.98527907332673 38.0 34.0 40.0 26.0 41.0 62-63 34.68801253393264 37.0 34.0 40.0 26.0 41.0 64-65 34.44159702923241 37.0 33.0 40.0 25.0 41.0 66-67 34.26032059802003 36.0 33.0 39.5 25.0 41.0 68-69 33.96135553353794 36.0 33.0 39.0 25.0 41.0 70-71 33.632111516557515 35.0 33.0 39.0 24.0 41.0 72-73 33.27398306791362 35.0 33.0 38.5 23.5 40.0 74-75 32.92962293484128 35.0 32.0 37.0 23.0 40.0 76-77 32.577681222461194 35.0 32.0 37.0 22.5 39.0 78-79 32.23274224080295 35.0 32.0 36.0 21.5 39.0 80-81 31.932577753075083 35.0 31.0 36.0 20.5 38.5 82-83 31.628675479084606 35.0 31.0 35.5 20.0 37.0 84-85 31.3699095398049 35.0 31.0 35.0 20.0 37.0 86-87 31.13627526320453 34.5 31.0 35.0 19.5 36.5 88-89 30.90753437567966 34.0 30.5 35.0 19.0 36.0 90-91 30.68767598656388 34.0 30.0 35.0 18.5 36.0 92-93 30.491240746558404 34.0 30.0 35.0 18.0 35.5 94-95 30.30763393828085 34.0 30.0 35.0 18.0 35.0 96-97 30.12723649339874 34.0 29.0 35.0 17.0 35.0 98-99 29.88481187018197 34.0 29.0 35.0 15.0 35.0 100 28.784479511531057 33.0 26.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5482754302995332 1101 2 -0.44889253982784894 1101 3 -0.4312258011313226 1101 4 -0.3194941375743099 1101 5 -0.2316067594729745 1101 6 -0.30174263374212984 1101 7 -0.2617509480718567 1101 8 -0.3037962849240543 1101 9 -0.4863628474032353 1101 10-11 -0.5391725636679254 1101 12-13 -0.5127885603436297 1101 14-15 -0.7387750024695734 1101 16-17 -0.7010466311913035 1101 18-19 -0.6448292014048818 1101 20-21 -0.6072150417702318 1101 22-23 -0.6859282598321954 1101 24-25 -0.7579871774272675 1101 26-27 -0.7617034620592236 1101 28-29 -0.7250480146676921 1101 30-31 -0.7165733206367619 1101 32-33 -0.7312000275772164 1101 34-35 -0.9171639694773219 1101 36-37 -0.8390494071785426 1101 38-39 -0.9431085778952593 1101 40-41 -1.0040070861960046 1101 42-43 -1.0067900245931227 1101 44-45 -0.9554455868464018 1101 46-47 -0.9840418473994959 1101 48-49 -1.0199705782988957 1101 50-51 -1.0423644875210343 1101 52-53 -0.9710926768371024 1101 54-55 -0.7420729085735971 1101 56-57 -0.6659770097177855 1101 58-59 -0.741959321188908 1101 60-61 -0.6057649612759732 1101 62-63 -0.7974131668292372 1101 64-65 -0.7968810981746444 1101 66-67 -0.803109613666237 1101 68-69 -0.7844483932783888 1101 70-71 -0.7953399921953661 1101 72-73 -0.874785281622259 1101 74-75 -0.8295573998705947 1101 76-77 -1.0164936037418784 1101 78-79 -0.9029567475599194 1101 80-81 -1.1519155964272123 1101 82-83 -1.1624532389130309 1101 84-85 -1.0224757381343004 1101 86-87 -1.1479660424654412 1101 88-89 -1.2402842189992889 1101 90-91 -1.2552018150936597 1101 92-93 -1.347168735012044 1101 94-95 -1.3434201881482188 1101 96-97 -1.3960798176116462 1101 98-99 -1.6557101229643365 1101 100 -1.4936407665049103 1102 1 -0.48859192931917406 1102 2 -0.5027437806478687 1102 3 -0.4944200280407003 1102 4 -0.3510140985920245 1102 5 -0.4507788500139682 1102 6 -0.42653815077947854 1102 7 -0.3414476751842628 1102 8 -0.42690953077929095 1102 9 -0.5132327788361835 1102 10-11 -0.5964480964838117 1102 12-13 -0.6600876449560715 1102 14-15 -0.7456369286120719 1102 16-17 -0.8338201742864584 1102 18-19 -0.8783394632590884 1102 20-21 -0.8567219622858175 1102 22-23 -0.7551519786956788 1102 24-25 -0.762518287842262 1102 26-27 -0.8668755758039666 1102 28-29 -0.8829210437999819 1102 30-31 -0.9385252192828304 1102 32-33 -0.8963886159776635 1102 34-35 -0.9512810091441182 1102 36-37 -0.9912148962553786 1102 38-39 -0.9621431862741332 1102 40-41 -1.0382668288665187 1102 42-43 -1.017975967767164 1102 44-45 -1.0834832465171402 1102 46-47 -1.0900434948650641 1102 48-49 -1.1130587641144558 1102 50-51 -1.1595224933915773 1102 52-53 -1.12955044022857 1102 54-55 -0.9054579862481162 1102 56-57 -0.7674951144362652 1102 58-59 -0.6931038456862524 1102 60-61 -0.7725087803796171 1102 62-63 -0.8365822408839705 1102 64-65 -0.8933900954250049 1102 66-67 -0.810454177609941 1102 68-69 -1.0148220895182192 1102 70-71 -0.9829747189133116 1102 72-73 -1.0805032633609102 1102 74-75 -1.023670030826704 1102 76-77 -1.1285172019459075 1102 78-79 -0.9656433926471095 1102 80-81 -1.0132477231660388 1102 82-83 -1.057788706990486 1102 84-85 -0.9812026649148997 1102 86-87 -1.11083773738266 1102 88-89 -1.258941186502451 1102 90-91 -1.2456482550597805 1102 92-93 -1.2030485628402872 1102 94-95 -1.2546565994577215 1102 96-97 -1.2526493766245608 1102 98-99 -1.4102812598315992 1102 100 -1.261517508235677 1103 1 -0.5309070380968599 1103 2 -0.4703162283177882 1103 3 -0.4957286014178699 1103 4 -0.34775557829953385 1103 5 -0.39033490202201904 1103 6 -0.4088358739278988 1103 7 -0.3776942152555449 1103 8 -0.3768626884790862 1103 9 -0.5091727864200593 1103 10-11 -0.6398603334502795 1103 12-13 -0.6001649353634022 1103 14-15 -0.7547983498468724 1103 16-17 -0.8749701007365758 1103 18-19 -0.632188438885187 1103 20-21 -0.6860209459046018 1103 22-23 -0.6619305381168559 1103 24-25 -0.7887767202518958 1103 26-27 -0.6466981601329493 1103 28-29 -0.7382895361535589 1103 30-31 -0.7688746968693962 1103 32-33 -0.9921873010639928 1103 34-35 -0.9597804937196912 1103 36-37 -0.7343048919787449 1103 38-39 -0.889225318562076 1103 40-41 -0.9286089061774945 1103 42-43 -1.0417976703299416 1103 44-45 -1.0392148248370532 1103 46-47 -1.0112756028077428 1103 48-49 -1.0612344970367147 1103 50-51 -1.0923447352466837 1103 52-53 -0.9136092110973806 1103 54-55 -0.7897112989302002 1103 56-57 -0.8060774659417689 1103 58-59 -0.8870837227138892 1103 60-61 -0.8607445820953146 1103 62-63 -0.7408241924416714 1103 64-65 -0.9192029323450086 1103 66-67 -1.0168181331025323 1103 68-69 -0.9324183194168469 1103 70-71 -0.8680169577671819 1103 72-73 -0.9797338721541422 1103 74-75 -0.8585836032944485 1103 76-77 -0.7460800534926584 1103 78-79 -0.8276966129420273 1103 80-81 -0.981961546454901 1103 82-83 -0.9695678380174684 1103 84-85 -1.1261089595065776 1103 86-87 -1.1150414268472169 1103 88-89 -1.0250709353900227 1103 90-91 -1.0312553799853923 1103 92-93 -1.1995718653852414 1103 94-95 -1.3235479510251587 1103 96-97 -1.2934589711810105 1103 98-99 -1.2491654730584365 1103 100 -1.5050805781431897 1104 1 -0.46161223136893526 1104 2 -0.3724729586781663 1104 3 -0.428400155545134 1104 4 -0.23874398795828 1104 5 -0.295945568473428 1104 6 -0.28023148385203456 1104 7 -0.32054548098085434 1104 8 -0.2617415953703315 1104 9 -0.4062730591402186 1104 10-11 -0.3566139710467482 1104 12-13 -0.3251148705792346 1104 14-15 -0.5755111916513656 1104 16-17 -0.5751070331876775 1104 18-19 -0.621310493341646 1104 20-21 -0.5169867864329944 1104 22-23 -0.5096052532677362 1104 24-25 -0.5956725195306092 1104 26-27 -0.5541130352480295 1104 28-29 -0.5774274222814171 1104 30-31 -0.6494888477046814 1104 32-33 -0.6039715876669263 1104 34-35 -0.7292646904308313 1104 36-37 -0.6907460809484789 1104 38-39 -0.6458973088716533 1104 40-41 -0.6361477793330579 1104 42-43 -0.7401563573503651 1104 44-45 -0.7925854130494443 1104 46-47 -0.7878474080820794 1104 48-49 -0.7145782487073618 1104 50-51 -0.7418538354188584 1104 52-53 -0.5058198163463672 1104 54-55 -0.5011303182493236 1104 56-57 -0.42805657002376307 1104 58-59 -0.493269510180518 1104 60-61 -0.5666557965715597 1104 62-63 -0.6019532909592726 1104 64-65 -0.49398908086579496 1104 66-67 -0.5514998197355183 1104 68-69 -0.6343319631075914 1104 70-71 -0.43517794078709926 1104 72-73 -0.4097342816651306 1104 74-75 -0.523668400983631 1104 76-77 -0.5616289852767409 1104 78-79 -0.5679611381612375 1104 80-81 -0.68462753382898 1104 82-83 -0.6660484981822314 1104 84-85 -0.6978104399183245 1104 86-87 -0.5791003463919999 1104 88-89 -0.7202041627697611 1104 90-91 -0.7424593765294496 1104 92-93 -0.7780407214841425 1104 94-95 -0.6955001772602749 1104 96-97 -0.8451904178842184 1104 98-99 -0.8205245338313993 1104 100 -0.7664110215326616 1105 1 -0.5533651382218778 1105 2 -0.45376088390365155 1105 3 -0.5137444439780623 1105 4 -0.36610240403864935 1105 5 -0.31371805707642153 1105 6 -0.3239186160841214 1105 7 -0.37150219795370276 1105 8 -0.42358759233751186 1105 9 -0.5869722685872318 1105 10-11 -0.5303898604487571 1105 12-13 -0.5518496991731254 1105 14-15 -0.6755788176429007 1105 16-17 -0.6799582440434406 1105 18-19 -0.6965550857745271 1105 20-21 -0.7172413678456877 1105 22-23 -0.6722623314708045 1105 24-25 -0.7171694979539325 1105 26-27 -0.7310715212871486 1105 28-29 -0.7191931772353541 1105 30-31 -0.6152139064781679 1105 32-33 -0.6682966101690511 1105 34-35 -0.6639839558352776 1105 36-37 -0.75604984403045 1105 38-39 -0.764309307514587 1105 40-41 -0.8032708885447803 1105 42-43 -0.8384694519339178 1105 44-45 -0.8516838065343748 1105 46-47 -0.8902934281136297 1105 48-49 -0.8760568067341268 1105 50-51 -0.9203274164214648 1105 52-53 -0.717308351390102 1105 54-55 -0.6173270363131778 1105 56-57 -0.5869259108180884 1105 58-59 -0.6123599220518727 1105 60-61 -0.6179280935296987 1105 62-63 -0.6731058708914475 1105 64-65 -0.7146909245323982 1105 66-67 -0.4991075009380097 1105 68-69 -0.5876160166762787 1105 70-71 -0.5204792227739716 1105 72-73 -0.5456050431675337 1105 74-75 -0.6317675256379616 1105 76-77 -0.615421017348666 1105 78-79 -0.7807480013248949 1105 80-81 -0.6239141555823231 1105 82-83 -0.8300823209875361 1105 84-85 -0.7891513462142754 1105 86-87 -0.7704664703759327 1105 88-89 -0.8153410608410141 1105 90-91 -0.9444827849559871 1105 92-93 -0.8700470015019235 1105 94-95 -0.9170570058225849 1105 96-97 -0.8231235053570565 1105 98-99 -0.9156759667655088 1105 100 -1.0644124360200564 1106 1 -0.4395951781644243 1106 2 -0.5067674898241599 1106 3 -0.5425672204981886 1106 4 -0.4271665483541369 1106 5 -0.3593509425389101 1106 6 -0.4303385301383713 1106 7 -0.39493592094363805 1106 8 -0.4978144843251755 1106 9 -0.5177205009776742 1106 10-11 -0.4824955661418855 1106 12-13 -0.5418646863490508 1106 14-15 -0.6791789546058524 1106 16-17 -0.8170738331984495 1106 18-19 -0.8295366167093121 1106 20-21 -0.8276992716745752 1106 22-23 -0.8349701308828799 1106 24-25 -0.788542247510108 1106 26-27 -0.8769865314895924 1106 28-29 -0.8033658322930677 1106 30-31 -0.8409636640019045 1106 32-33 -0.8827100419083678 1106 34-35 -0.8268817738978456 1106 36-37 -0.9121225659747267 1106 38-39 -0.8775068441751088 1106 40-41 -0.8276509667153462 1106 42-43 -0.7938279031161954 1106 44-45 -0.7642019611031472 1106 46-47 -0.8744626939862314 1106 48-49 -0.8775352141361026 1106 50-51 -0.9208561043781955 1106 52-53 -0.7491526113565499 1106 54-55 -0.7450238059213561 1106 56-57 -0.787939601132301 1106 58-59 -0.705205152920179 1106 60-61 -0.7822043878744438 1106 62-63 -0.6831025963405395 1106 64-65 -0.6588594577429276 1106 66-67 -0.7860504908571784 1106 68-69 -0.8294262751415147 1106 70-71 -0.6849380596255799 1106 72-73 -0.7272196360017986 1106 74-75 -0.773198095688926 1106 76-77 -0.7885303753871611 1106 78-79 -0.8266386891400757 1106 80-81 -0.6824094969480221 1106 82-83 -0.6280421561535263 1106 84-85 -0.6275829110907516 1106 86-87 -0.7294598225890212 1106 88-89 -0.7128771481862977 1106 90-91 -0.907450330518099 1106 92-93 -0.771205654241033 1106 94-95 -0.9380665892418918 1106 96-97 -0.8110263573563259 1106 98-99 -0.8523343398968457 1106 100 -1.1613005161563414 1107 1 -0.42100307375142165 1107 2 -0.501630835433776 1107 3 -0.5552150204594213 1107 4 -0.2810303462477819 1107 5 -0.2507543152855618 1107 6 -0.27415516126843187 1107 7 -0.27889276012435715 1107 8 -0.3486576709892617 1107 9 -0.5194327191078045 1107 10-11 -0.5005869275168351 1107 12-13 -0.5987717096739829 1107 14-15 -0.6100521858522185 1107 16-17 -0.7412758749377986 1107 18-19 -0.7113451067267604 1107 20-21 -0.6337098034313726 1107 22-23 -0.7146948837419558 1107 24-25 -0.7675433459141914 1107 26-27 -0.8195787651266002 1107 28-29 -0.7641948043160625 1107 30-31 -0.7832409141149768 1107 32-33 -0.7912032899538559 1107 34-35 -0.8286909100353341 1107 36-37 -0.9194156460290017 1107 38-39 -0.8161496690119563 1107 40-41 -0.8393780456066011 1107 42-43 -0.8458486435697807 1107 44-45 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0.8787937694919563 2205 74-75 0.8269326228294531 2205 76-77 0.8410106962157045 2205 78-79 0.8669309641168539 2205 80-81 0.8587708275152046 2205 82-83 0.9095486379970907 2205 84-85 0.9135959987792468 2205 86-87 0.9361379168101678 2205 88-89 0.8867955957749558 2205 90-91 0.9102476049235051 2205 92-93 0.9887269120998532 2205 94-95 1.0286685942581322 2205 96-97 0.9818312705042089 2205 98-99 0.9948277536930448 2205 100 1.1113775856778005 2206 1 0.7976192919803324 2206 2 0.7905347272400078 2206 3 0.7952527213837257 2206 4 0.6127831905942784 2206 5 0.4972812071406594 2206 6 0.6178856893308904 2206 7 0.6341057303538236 2206 8 0.6536776031578384 2206 9 0.807211199771146 2206 10-11 0.8430759433990076 2206 12-13 0.9683163254095106 2206 14-15 1.1200384904173788 2206 16-17 1.11788998544192 2206 18-19 1.1523578590733194 2206 20-21 1.116817145636098 2206 22-23 1.1287658906370126 2206 24-25 1.1687219139010452 2206 26-27 1.1586442357111721 2206 28-29 1.2143354443256555 2206 30-31 1.1858568746550233 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2311 38-39 1.0276581725291507 2311 40-41 1.046218989781302 2311 42-43 1.0787375667649926 2311 44-45 1.07984419146279 2311 46-47 1.1277032653587966 2311 48-49 1.1093810408925648 2311 50-51 1.0716143647731684 2311 52-53 1.0269064271498536 2311 54-55 0.9405884399510072 2311 56-57 0.9226331861514652 2311 58-59 0.9017818479133339 2311 60-61 0.9064786024385967 2311 62-63 0.8688354103629834 2311 64-65 0.8001428299685003 2311 66-67 0.8413328829237727 2311 68-69 0.803491191269238 2311 70-71 0.7765009749792995 2311 72-73 0.8480671464282494 2311 74-75 0.834185537284128 2311 76-77 0.7738905880708415 2311 78-79 0.7540638442581908 2311 80-81 0.7214070386902058 2311 82-83 0.7750104448393174 2311 84-85 0.8201844216996399 2311 86-87 0.8774835248361761 2311 88-89 0.973933520161026 2311 90-91 1.0120718829279696 2311 92-93 1.0704849194875266 2311 94-95 1.0508341906535286 2311 96-97 0.9503139796207023 2311 98-99 1.1379336783884746 2311 100 1.0522171709769523 2312 1 0.6088981857612907 2312 2 0.6344754414820386 2312 3 0.6608610959938872 2312 4 0.37566653004270023 2312 5 0.41055438331628835 2312 6 0.5016073306199473 2312 7 0.3884118974179671 2312 8 0.45754845254327137 2312 9 0.6484677734885054 2312 10-11 0.5701799151473139 2312 12-13 0.7333405182181778 2312 14-15 0.9774409930162875 2312 16-17 1.0021157391262676 2312 18-19 0.9788875860347304 2312 20-21 0.9460168719860036 2312 22-23 0.9906014483328462 2312 24-25 0.9349825006435353 2312 26-27 0.9752486049400559 2312 28-29 1.0160014230451537 2312 30-31 1.0648570963268824 2312 32-33 1.0661397502797598 2312 34-35 0.9800421661843544 2312 36-37 0.9661626384379929 2312 38-39 0.9856074175906002 2312 40-41 0.9950942937834029 2312 42-43 1.0468391034657998 2312 44-45 1.0555172804221442 2312 46-47 1.0568927570196038 2312 48-49 1.1364694906010797 2312 50-51 1.260663993115699 2312 52-53 1.1584630371092004 2312 54-55 1.0877176691566675 2312 56-57 0.9938112911454411 2312 58-59 0.9375874832777171 2312 60-61 0.8888829162879759 2312 62-63 1.0223052441155644 2312 64-65 1.0589942087074249 2312 66-67 1.0720443223438991 2312 68-69 1.030511613057243 2312 70-71 0.9088598375345853 2312 72-73 0.9598122078843616 2312 74-75 1.01874088950931 2312 76-77 1.008294796115571 2312 78-79 0.9295561711856237 2312 80-81 0.9521990982746154 2312 82-83 1.0531487449311143 2312 84-85 1.0815139763663701 2312 86-87 0.9809053739438447 2312 88-89 0.9364603066204147 2312 90-91 1.0150543819444344 2312 92-93 1.035812921359227 2312 94-95 1.153989628118513 2312 96-97 1.1648323151198028 2312 98-99 0.8823254357590322 2312 100 1.0660398781454425 2313 1 0.6139652699960578 2313 2 0.6362413823478832 2313 3 0.6272897546581362 2313 4 0.525194439643279 2313 5 0.33650365286289485 2313 6 0.5453089148198274 2313 7 0.6254357360203358 2313 8 0.5674770356032681 2313 9 0.7234612308128092 2313 10-11 0.792036668351777 2313 12-13 0.7573455226381185 2313 14-15 0.9091334959822959 2313 16-17 0.9925737555033152 2313 18-19 0.9415717260072825 2313 20-21 0.943061690768431 2313 22-23 1.0069478873725544 2313 24-25 0.9897792269794081 2313 26-27 0.9827417133216656 2313 28-29 1.0008414618941046 2313 30-31 1.0156201904018403 2313 32-33 1.1381607246441092 2313 34-35 1.1669592137801388 2313 36-37 1.225611716066112 2313 38-39 1.1789304716534588 2313 40-41 1.1287607947022806 2313 42-43 1.2490915286175834 2313 44-45 1.1969413592134046 2313 46-47 1.2869034288506285 2313 48-49 1.3490844963004776 2313 50-51 1.3919358747310753 2313 52-53 1.3683357893366122 2313 54-55 1.1472184339227525 2313 56-57 1.1052549398066631 2313 58-59 1.0692112881489564 2313 60-61 1.0649822311971349 2313 62-63 1.1418097600157253 2313 64-65 1.097471248277536 2313 66-67 1.1789599305510379 2313 68-69 1.1229546772394414 2313 70-71 1.1896277150324295 2313 72-73 1.2165406955666782 2313 74-75 1.120008423880158 2313 76-77 0.9086837945336939 2313 78-79 1.0630880383780337 2313 80-81 1.1079704651995037 2313 82-83 1.148787978885565 2313 84-85 1.2203663474320372 2313 86-87 1.22650673307567 2313 88-89 1.237329682827177 2313 90-91 1.224827685501321 2313 92-93 1.3611162834221737 2313 94-95 1.3338383323779404 2313 96-97 1.3604417435516538 2313 98-99 1.3836566521733005 2313 100 1.5282613055537269 2314 1 0.5259105821936103 2314 2 0.47034601500789464 2314 3 0.5102056234717338 2314 4 0.32959958124172317 2314 5 0.3723859468281958 2314 6 0.4249609069460192 2314 7 0.44112100618852423 2314 8 0.4237096205677986 2314 9 0.5742118331112209 2314 10-11 0.5741872930843712 2314 12-13 0.6015874803288241 2314 14-15 0.8251497200700655 2314 16-17 0.844593974067898 2314 18-19 0.7767834263301907 2314 20-21 0.8319906170740978 2314 22-23 0.8297835589241203 2314 24-25 0.8285488804704499 2314 26-27 0.7784389649349919 2314 28-29 0.8294576595770309 2314 30-31 0.8999721947377921 2314 32-33 0.7645364317925711 2314 34-35 0.9115757529392212 2314 36-37 0.7682309671811183 2314 38-39 0.7970154555375686 2314 40-41 0.9094015786679961 2314 42-43 0.9746191539388391 2314 44-45 1.0715925621723414 2314 46-47 1.0709971918483134 2314 48-49 1.0596085132908968 2314 50-51 1.0745300710262526 2314 52-53 0.983220178813994 2314 54-55 0.885406734195449 2314 56-57 0.8226112096165039 2314 58-59 0.8332956903324202 2314 60-61 0.8067366741095654 2314 62-63 0.8541930352286258 2314 64-65 0.971144181627146 2314 66-67 0.8160397734323013 2314 68-69 0.8516630437029065 2314 70-71 0.8232056902178329 2314 72-73 0.8830601999100836 2314 74-75 0.9380063511993484 2314 76-77 0.9307832077704674 2314 78-79 0.9339326260306962 2314 80-81 0.8249470413253555 2314 82-83 0.8568010542455795 2314 84-85 0.9245767781386895 2314 86-87 0.8411459347362005 2314 88-89 0.9811171000266548 2314 90-91 1.0106256030924072 2314 92-93 1.1385666477792107 2314 94-95 1.0827645189331072 2314 96-97 1.1328057015381745 2314 98-99 1.169213104434636 2314 100 1.235057872130163 2315 1 0.38739712510443525 2315 2 0.42896190437928183 2315 3 0.4199555060143467 2315 4 0.09957415463375696 2315 5 0.2737834765269298 2315 6 0.33443278176036983 2315 7 0.31877984708444984 2315 8 0.2832365789666369 2315 9 0.48897409985868734 2315 10-11 0.32603787672090334 2315 12-13 0.4549029568041192 2315 14-15 0.6585350374424976 2315 16-17 0.6397987530101759 2315 18-19 0.5025724943616083 2315 20-21 0.5971951909460387 2315 22-23 0.4337215930099063 2315 24-25 0.5311177141621428 2315 26-27 0.601037089154481 2315 28-29 0.6636551893885141 2315 30-31 0.6968613693000734 2315 32-33 0.7363318606689973 2315 34-35 0.6749136229546409 2315 36-37 0.762694984136985 2315 38-39 0.8644912785549579 2315 40-41 0.8817613385679763 2315 42-43 0.920022981481587 2315 44-45 0.9074921758015009 2315 46-47 0.9063007977812063 2315 48-49 1.057836451862542 2315 50-51 0.87815034474513 2315 52-53 0.8414474422743439 2315 54-55 0.7548436286609572 2315 56-57 0.7112180935106736 2315 58-59 0.730467643503367 2315 60-61 0.6675895073888611 2315 62-63 0.7546665856297281 2315 64-65 0.8841576423455706 2315 66-67 0.8366964875867282 2315 68-69 0.8128706063869586 2315 70-71 0.7851438247951421 2315 72-73 0.48468287710970515 2315 74-75 0.7546423900353005 2315 76-77 0.8216192978669525 2315 78-79 0.8806101447472372 2315 80-81 0.6115828046200669 2315 82-83 0.5314306397786908 2315 84-85 0.7901591606374332 2315 86-87 0.8815929584784818 2315 88-89 0.9349870556927016 2315 90-91 0.975864700661706 2315 92-93 1.1117932703066593 2315 94-95 1.0971046790415997 2315 96-97 0.8908095933644873 2315 98-99 0.27916167265255254 2315 100 0.7136458860295605 2316 1 0.33167524687150163 2316 2 0.3261717068405794 2316 3 0.3368984481932529 2316 4 0.20701920954382302 2316 5 0.13261615877502209 2316 6 0.16169711010487475 2316 7 0.1584822690309906 2316 8 0.20540230635080547 2316 9 0.23214852990380308 2316 10-11 0.2791201180767118 2316 12-13 -0.010965903444592584 2316 14-15 0.04929227549771298 2316 16-17 0.359018260124941 2316 18-19 0.32095342528780435 2316 20-21 0.34510743530599086 2316 22-23 0.31711423554621376 2316 24-25 0.3787289574776551 2316 26-27 0.4291479674594356 2316 28-29 0.3613286380299954 2316 30-31 0.24260796693335607 2316 32-33 0.46302053504038554 2316 34-35 0.440252485618835 2316 36-37 0.4782426648164986 2316 38-39 0.6157942508969398 2316 40-41 0.6896188009217994 2316 42-43 0.6674623535757078 2316 44-45 0.5664097677535196 2316 46-47 0.4512782129744366 2316 48-49 0.31790257827491075 2316 50-51 0.43529904702966604 2316 52-53 0.4804902486231839 2316 54-55 0.2426619763737321 2316 56-57 0.12594758878294954 2316 58-59 0.17232699550670105 2316 60-61 0.25783017662534036 2316 62-63 0.32531498276008364 2316 64-65 0.2860203211169008 2316 66-67 0.1787625987166308 2316 68-69 0.24076683669608911 2316 70-71 -0.07356475811064911 2316 72-73 -0.06175656134997354 2316 74-75 0.16145238035781517 2316 76-77 0.38958261398816774 2316 78-79 0.3584117864527627 2316 80-81 0.44803016591003697 2316 82-83 0.4967630922944224 2316 84-85 0.6167186556592412 2316 86-87 0.7073717384531797 2316 88-89 0.657775197172743 2316 90-91 0.5578795433392543 2316 92-93 0.517992315463399 2316 94-95 0.6971316497766544 2316 96-97 0.6968865414659149 2316 98-99 0.40960578839804285 2316 100 0.3180040936162065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 11.0 1 173.0 2 66.0 3 56.0 4 53.0 5 37.0 6 52.0 7 335.0 8 1208.0 9 2374.0 10 3128.0 11 4246.0 12 5172.0 13 6176.0 14 7139.0 15 8298.0 16 10041.0 17 13214.0 18 16964.0 19 20367.0 20 23922.0 21 29048.0 22 31889.0 23 32302.0 24 31241.0 25 31385.0 26 33945.0 27 38528.0 28 45335.0 29 54094.0 30 66114.0 31 83382.0 32 105615.0 33 140524.0 34 200561.0 35 281733.0 36 421719.0 37 615681.0 38 578814.0 39 158410.0 40 223.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.61861868638836 18.600697007895338 12.272948549727095 23.507735755989206 2 16.316672629938292 20.030365903980897 40.66775952088204 22.98520194519877 3 19.32457943316853 26.431016469475104 29.39726864393179 24.84713545342457 4 12.588758645448165 16.32437212342068 38.48614182434345 32.60072740678771 5 12.975001530781602 36.80411474094341 34.4967595770531 15.724124151221886 6 29.55959433665132 36.46421431981692 19.353052130235486 14.623139213296277 7 26.80330344900735 32.241801583507126 23.00369876160961 17.951196205875913 8 25.929422944941077 34.61801681627974 20.93021756460838 18.5223426741708 9 26.600317370370835 16.252805517332078 21.570322550283347 35.57655456201374 10-11 25.29821180895233 25.772536760949315 28.347100591830415 20.58215083826794 12-13 25.48574913461405 23.96642827925591 28.437699141914912 22.11012344421512 14-15 22.845615401608722 25.182317972469782 26.81393471825007 25.15813190767143 16-17 21.933477008600374 27.42880621966203 27.700521662067718 22.937195109669883 18-19 21.427710532760997 26.874524665815898 29.92903824587184 21.76872655555126 20-21 22.563307956546105 26.35733799295964 29.562335016775883 21.517019033718373 22-23 22.424640788645494 26.24212857966527 29.395192596199806 21.938038035489434 24-25 21.877872856833694 26.214522005771133 29.909230413974992 21.998374723420177 26-27 21.80630457666962 26.330573827561853 29.499104343607215 22.364017252161315 28-29 21.60610856469419 26.799703388888297 29.60050956267908 21.993678483738435 30-31 22.431136088179837 26.479377322063357 29.57169139506038 21.517795194696422 32-33 22.30619730642693 26.623039800290343 29.197563121907233 21.873199771375496 34-35 22.463923534474407 26.64377079614688 29.310785740447976 21.58151992893074 36-37 22.293335000592997 26.57683338403865 29.097236903847246 22.03259471152111 38-39 22.253855156432362 26.910046537904442 29.081509251908138 21.75458905375506 40-41 22.313630080497717 26.24134618142652 29.45609817646587 21.988925561609904 42-43 22.10645281324015 26.564486474108122 28.98832230207758 22.340738410574147 44-45 22.021020167266535 27.069605854135283 28.918314931178067 21.99105904742012 46-47 22.05252055331483 26.678644280659768 28.485472777817893 22.783362388207504 48-49 22.1665197371708 26.70091977327194 28.63911590883817 22.493444580719093 50-51 22.142812251731932 27.02361940797584 28.38744402134239 22.446124318949835 52-53 22.105695760855724 27.274251022194147 28.10319124418393 22.516861972766204 54-55 22.019504990833163 27.103666476711595 28.30879603724495 22.568032495210296 56-57 22.36590859569706 27.015238992843045 28.01783824065992 22.601014170799978 58-59 22.08268690038072 27.136532764117753 28.417154990150795 22.363625345350727 60-61 22.257714166931205 27.454635106892113 28.089332703813696 22.198318022362987 62-63 22.469483714844976 27.113370281556026 27.976603791949266 22.440542211649728 64-65 22.1221078305855 26.979577899533634 28.06851595127624 22.82979831860462 66-67 22.063573305554687 26.98644018303223 27.96035805832678 22.989628453086308 68-69 21.791764627765016 26.975553293523575 28.175132203162562 23.057549875548847 70-71 21.897660697601967 26.726860694443076 28.22059618228917 23.15488242566578 72-73 22.040407801118384 26.995444431819188 28.05279075895782 22.91135700810461 74-75 22.26956683865751 27.08389230759562 27.846369459144967 22.80017139460191 76-77 22.09565388615263 26.92894473343197 27.853443416793986 23.12195796362141 78-79 21.92122294652187 27.059383708817037 27.82609396237026 23.19329938229083 80-81 21.968350458947995 27.131221322800247 27.824695111634213 23.075733106617548 82-83 22.40944076463542 26.889746562082962 28.254987400776482 22.44582527250514 84-85 22.449636254452866 27.180721059023117 27.622958175908447 22.746684510615577 86-87 21.91189867674322 27.352339231697066 27.900019882849094 22.83574220871062 88-89 22.14624873028395 26.78593296414889 27.955070743164157 23.112747562402998 90-91 22.40539123412042 26.798208679897247 27.885314778165256 22.91108530781708 92-93 22.43195038110558 26.93054795317388 27.866880287101743 22.770621378618802 94-95 21.89863579986403 26.861927167353045 28.115778226070905 23.123658806712022 96-97 22.04212611665503 26.856184935814643 27.865305745573544 23.23638320195678 98-99 21.968441737041978 26.96974863420383 27.627193645418014 23.434615983336172 100 22.385607854714596 26.691565025583774 27.423722455062418 23.499104664639216 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1464.0 1 1029.0 2 1987.0 3 2867.0 4 1671.5 5 1076.5 6 1457.0 7 2519.0 8 3910.0 9 4467.5 10 3791.0 11 3161.5 12 3194.5 13 3445.5 14 3864.5 15 4241.5 16 4496.0 17 4829.5 18 5259.0 19 5649.5 20 6066.5 21 6574.0 22 7435.0 23 8676.0 24 10211.5 25 12181.0 26 15127.0 27 18550.0 28 22057.0 29 26988.0 30 33236.5 31 39674.5 32 47510.5 33 55282.5 34 62800.5 35 69985.0 36 76652.5 37 85602.0 38 90565.5 39 90893.5 40 90055.5 41 88644.5 42 89698.0 43 93327.0 44 98548.0 45 106942.0 46 113902.0 47 118009.5 48 122237.0 49 124215.0 50 123818.5 51 123811.0 52 120967.5 53 115374.0 54 110180.5 55 101266.0 56 92034.5 57 81843.5 58 71110.0 59 62910.5 60 50511.5 61 37598.0 62 29234.0 63 22862.0 64 16930.0 65 11552.5 66 8274.0 67 6112.5 68 4775.5 69 3807.0 70 2984.0 71 2383.0 72 1886.5 73 1521.0 74 1195.5 75 895.0 76 687.0 77 478.5 78 325.5 79 232.5 80 168.5 81 120.5 82 90.0 83 74.5 84 61.0 85 44.0 86 34.0 87 27.5 88 22.0 89 18.0 90 13.5 91 11.0 92 7.0 93 3.5 94 5.5 95 7.5 96 4.5 97 2.5 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056225481903933366 2 0.1303657878414409 3 0.01614267417413789 4 0.016013790547997068 5 0.01884923032309514 6 0.051360125017117356 7 0.055613284679764466 8 0.047106965354470245 9 0.03885841328145768 10-11 0.037569577020049455 12-13 0.038729529655316855 14-15 0.017673167234560142 16-17 0.016513214599292753 18-19 0.015949348734926656 20-21 0.019590311173404866 22-23 0.02371458720991115 24-25 0.023150721345545057 26-27 0.017512062701884115 28-29 0.0168193132113772 30-31 0.01488605881926488 32-33 0.0216041178318552 34-35 0.022119652336418487 36-37 0.021877995537404445 38-39 0.019993072505094932 40-41 0.02429456352754485 42-43 0.030658192568247908 44-45 0.039341726879485756 46-47 0.045077048242752306 48-49 0.03802066971154233 50-51 0.02674335242422046 52-53 0.03802066971154233 54-55 0.03502412540376823 56-57 0.036699612543598915 58-59 0.04169385305655575 60-61 0.02191021644393965 62-63 0.0246328830461645 64-65 0.02369847675664355 66-67 0.030674303021515508 68-69 0.03715070523509179 70-71 0.039261174613147744 72-73 0.042773253425485123 74-75 0.025567289335685462 76-77 0.0192036602949824 78-79 0.0186075735240811 80-81 0.023134610892277453 82-83 0.019525869360334454 84-85 0.012098950403969615 86-87 0.012823920801011737 88-89 0.016722650491771584 90-91 0.019719194799545686 92-93 0.018156480832588223 94-95 0.022377419588700126 96-97 0.023376267691291495 98-99 0.025970050667375528 100 0.02664668970461484 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3103575.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.85711791650571 #Duplication Level Percentage of deduplicated Percentage of total 1 83.6163427574642 60.08429401270063 2 10.877634082615478 15.632708698542036 3 2.699603821040607 5.819572502890928 4 1.0284279943418406 2.9559948663222846 5 0.5108352407777708 1.8353574066237432 6 0.31914756463501115 1.3759814510846173 7 0.20155847924506098 1.013838798712874 8 0.14137559075259434 0.8127073996179861 9 0.10477998884350748 0.6776269212256263 >10 0.46903947037737836 5.773625360436036 >50 0.0216953274826637 1.066109649921414 >100 0.008618846495335716 1.116544267527413 >500 4.456591241704207E-4 0.2184733451446242 >1k 3.4662376309484784E-4 0.5553279310332637 >5k 4.9517680442121126E-5 0.24064091738181342 >10k+ 9.903536088424225E-5 0.82119647083467 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13054 0.42061171391057084 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10025 0.3230145880154338 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6763 0.2179099908975939 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4697 0.1513415979958596 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.222090653520537E-5 2 0.0 0.0 0.0 0.0 3.222090653520537E-5 3 0.0 0.0 0.0 0.0 3.222090653520537E-5 4 0.0 0.0 0.0 0.0 3.222090653520537E-5 5 0.0 0.0 0.0 0.0 3.222090653520537E-5 6 0.0 0.0 0.0 0.0 3.222090653520537E-5 7 0.0 0.0 0.0 0.0 3.222090653520537E-5 8 0.0 0.0 0.0 0.0 3.222090653520537E-5 9 0.0 0.0 0.0 0.0 3.222090653520537E-5 10-11 0.0 0.0 0.0 0.0 3.222090653520537E-5 12-13 0.0 0.0 0.0 0.0 3.222090653520537E-5 14-15 0.0 0.0 0.0 0.0 3.222090653520537E-5 16-17 0.0 0.0 0.0 0.0 3.222090653520537E-5 18-19 0.0 0.0 0.0 0.0 3.222090653520537E-5 20-21 0.0 0.0 0.0 8.055226633801342E-5 3.222090653520537E-5 22-23 0.0 0.0 0.0 4.833135980280805E-4 3.222090653520537E-5 24-25 0.0 0.0 0.0 9.18295836253353E-4 3.222090653520537E-5 26-27 0.0 0.0 0.0 0.0017721498594362953 3.222090653520537E-5 28-29 0.0 0.0 0.0 0.004655920994337176 3.222090653520537E-5 30-31 0.0 0.0 0.0 0.014902169272532483 3.222090653520537E-5 32-33 0.0 0.0 0.0 0.04050167951475315 3.222090653520537E-5 34-35 0.0 0.0 0.0 0.07836124469361946 4.833135980280805E-5 36-37 0.0 0.0 0.0 0.1403864897738898 6.444181307041074E-5 38-39 0.0 0.0 0.0 0.23253828246457714 6.444181307041074E-5 40-41 0.0 1.6110453267602684E-5 0.0 0.33960835488106456 6.444181307041074E-5 42-43 0.0 3.222090653520537E-5 0.0 0.4417808495042008 6.444181307041074E-5 44-45 0.0 3.222090653520537E-5 0.0 0.5624642549318125 6.444181307041074E-5 46-47 0.0 3.222090653520537E-5 0.0 0.6994353286129705 6.444181307041074E-5 48-49 0.0 3.222090653520537E-5 0.0 0.8490692185624642 6.444181307041074E-5 50-51 0.0 3.222090653520537E-5 0.0 1.0043900985154217 8.055226633801342E-5 52-53 0.0 3.222090653520537E-5 0.0 1.1544750811564084 1.2888362614082148E-4 54-55 0.0 3.222090653520537E-5 0.0 1.3304656726517001 1.6110453267602684E-4 56-57 0.0 3.222090653520537E-5 0.0 1.5362767131453243 1.6110453267602684E-4 58-59 0.0 3.222090653520537E-5 0.0 1.7591648341026076 1.6110453267602684E-4 60-61 0.0 3.222090653520537E-5 0.0 1.981231321943243 1.6110453267602684E-4 62-63 0.0 3.222090653520537E-5 0.0 2.2084853757360463 1.6110453267602684E-4 64-65 0.0 3.222090653520537E-5 0.0 2.441974174943412 1.6110453267602684E-4 66-67 0.0 3.222090653520537E-5 0.0 2.7033179478504628 1.6110453267602684E-4 68-69 0.0 3.222090653520537E-5 0.0 2.998413120353141 1.6110453267602684E-4 70-71 0.0 3.222090653520537E-5 0.0 3.2975197957194524 1.7721498594362953E-4 72-73 0.0 3.222090653520537E-5 0.0 3.5907622660963563 2.5776725228164295E-4 74-75 0.0 3.222090653520537E-5 0.0 3.889095639705823 2.5776725228164295E-4 76-77 0.0 3.222090653520537E-5 0.0 4.224611939456917 2.5776725228164295E-4 78-79 0.0 3.222090653520537E-5 0.0 4.591608064892906 2.5776725228164295E-4 80-81 0.0 3.222090653520537E-5 0.0 4.963695093561458 2.5776725228164295E-4 82-83 0.0 3.222090653520537E-5 0.0 5.3530847490394144 2.899881588168483E-4 84-85 0.0 3.222090653520537E-5 0.0 5.757118806537622 2.899881588168483E-4 86-87 0.0 3.222090653520537E-5 0.0 6.171785763192448 2.899881588168483E-4 88 0.0 3.222090653520537E-5 0.0 6.506560982093231 2.899881588168483E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5355 0.0 50.098755 1 TACCTGG 2715 0.0 46.97599 2 GTATCAA 9745 0.0 40.76356 1 GTACCTG 3485 0.0 37.275017 1 ACCTGGG 3460 0.0 35.592815 3 ATCAACG 11845 0.0 32.97639 3 CAACGCA 11835 0.0 32.924824 5 TATCAAC 12400 0.0 31.767298 2 TATCACG 260 0.0 30.77174 2 TCAACGC 12735 0.0 30.708708 4 AACGCAG 13040 0.0 30.676313 6 GTACTAG 1300 0.0 30.050123 1 GTACATG 10285 0.0 29.974264 1 TACATGG 10390 0.0 29.080103 2 ACGCAGA 14050 0.0 27.668167 7 ACATGGG 10560 0.0 27.374638 3 CGCAGAG 14500 0.0 26.842348 8 CCTGGGG 3505 0.0 26.41894 4 CATGGGG 7850 0.0 26.406214 4 GTACACG 535 0.0 26.392406 1 >>END_MODULE