FastQCFastQC Report
Mon 6 Jun 2016
ERR1211181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103575
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120970.38977630635637933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102760.33110203555577034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68090.21939215259821335No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39210.12633817452454024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57150.051.4096951
TACCTGG31200.050.003082
GTACCTG35900.043.7354161
ACCTGGG37650.040.5664183
GTATCAA100850.039.154771
CCTGGGG33900.032.4387744
ATCAACG123050.031.202453
TATCAAC127350.030.7737462
CAACGCA125150.030.708515
ATAACGC4050.030.1693943
TCAACGC132350.029.1164324
TAACGCA4200.029.0919154
AACGCAG136200.028.8380226
GTACATG103050.026.6863461
GTGGTAT24050.026.5830671
TACATGG103750.026.043122
ACGCAGA147400.026.009327
TGGTATC25650.025.8312242
CTGGGGG24200.025.820975
CGCAGAG149700.025.546538
Produced by FastQC (version 0.11.5)