##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211181_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3103575 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03833127925054 33.0 31.0 34.0 30.0 34.0 2 32.17280329942083 33.0 31.0 34.0 30.0 34.0 3 32.24688915202629 34.0 31.0 34.0 30.0 34.0 4 35.89892591608065 37.0 35.0 37.0 35.0 37.0 5 35.78057659312245 37.0 35.0 37.0 35.0 37.0 6 35.8124846346552 37.0 35.0 37.0 35.0 37.0 7 35.75221704002642 37.0 35.0 37.0 33.0 37.0 8 35.73467501188146 37.0 35.0 37.0 33.0 37.0 9 37.320017399289526 39.0 37.0 39.0 34.0 39.0 10-11 37.28980546627679 39.0 37.0 39.0 34.0 39.0 12-13 37.19080511990205 39.0 37.0 39.0 33.0 39.0 14-15 38.374211353036415 40.0 38.0 41.0 33.5 41.0 16-17 38.32297108979161 40.0 38.0 41.0 33.0 41.0 18-19 38.35780511184682 40.0 38.0 41.0 33.5 41.0 20-21 38.31815905850512 40.0 38.0 41.0 34.0 41.0 22-23 38.17503250283947 40.0 37.5 41.0 33.0 41.0 24-25 38.105645102824965 40.0 37.0 41.0 33.0 41.0 26-27 37.95823332769467 40.0 37.0 41.0 33.0 41.0 28-29 37.91540175442836 40.0 37.0 41.0 33.0 41.0 30-31 37.90019316433468 40.0 37.0 41.0 33.0 41.0 32-33 37.73690002658225 40.0 37.0 41.0 33.0 41.0 34-35 37.55653899132452 40.0 36.0 41.0 32.5 41.0 36-37 37.38107521165108 40.0 36.0 41.0 32.0 41.0 38-39 37.27650677041798 40.0 36.0 41.0 31.5 41.0 40-41 37.17337586492996 39.0 36.0 41.0 31.0 41.0 42-43 37.01844872445486 39.0 36.0 41.0 31.0 41.0 44-45 36.8688224064184 39.0 35.0 41.0 31.0 41.0 46-47 36.62091104613228 39.0 35.0 41.0 30.0 41.0 48-49 36.38060285316128 39.0 35.0 40.5 29.5 41.0 50-51 36.1800650862312 39.0 35.0 40.0 29.0 41.0 52-53 35.97916789508873 38.5 35.0 40.0 28.5 41.0 54-55 35.737252845508806 38.0 35.0 40.0 28.0 41.0 56-57 35.49270260908791 38.0 34.0 40.0 27.5 41.0 58-59 35.229158470473564 38.0 34.0 40.0 27.0 41.0 60-61 34.96097742119974 37.0 34.0 40.0 26.0 41.0 62-63 34.658011325648644 37.0 33.5 40.0 26.0 41.0 64-65 34.28428376952385 36.0 33.0 39.0 26.0 41.0 66-67 33.97341034129995 36.0 33.0 39.0 26.0 40.0 68-69 33.641724462917765 35.0 32.5 38.5 25.0 40.0 70-71 33.306860797628545 35.0 32.0 38.0 25.0 40.0 72-73 32.95405024850374 35.0 32.0 37.0 24.0 39.5 74-75 32.606995319913324 35.0 31.5 37.0 24.0 39.0 76-77 31.633944725034837 34.0 30.5 36.0 22.5 38.0 78-79 32.143544460823406 34.5 31.0 36.0 24.0 38.0 80-81 32.06807810347745 35.0 31.0 35.5 24.0 37.0 82-83 31.814703688488276 34.0 31.0 35.0 24.0 37.0 84-85 31.54785771247674 34.0 31.0 35.0 23.0 36.5 86-87 31.301072956187625 34.0 31.0 35.0 23.0 36.0 88-89 31.077328403534633 34.0 31.0 35.0 21.0 36.0 90-91 30.856009762934683 34.0 30.0 35.0 20.0 35.0 92-93 30.67268617642557 34.0 30.0 35.0 20.0 35.0 94-95 30.47120739792014 34.0 30.0 35.0 19.5 35.0 96-97 30.289158470473566 34.0 29.0 35.0 19.0 35.0 98-99 30.098379127296745 34.0 29.0 35.0 19.0 35.0 100 28.343313114714483 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4433999560529074 1101 2 -0.44641203069841495 1101 3 -0.5306644173794659 1101 4 -0.30770313426713614 1101 5 -0.362215698429992 1101 6 -0.45924467627915533 1101 7 -0.4853795263485168 1101 8 -0.32561816473620553 1101 9 -0.5973631896039038 1101 10-11 -0.5125120827158298 1101 12-13 -0.6159650542253345 1101 14-15 -0.8537492929455723 1101 16-17 -0.7983900340604464 1101 18-19 -0.8714328319027373 1101 20-21 -0.8922850122677346 1101 22-23 -0.9152609860455314 1101 24-25 -0.9247315205971631 1101 26-27 -1.0643588333750387 1101 28-29 -1.22424431607881 1101 30-31 -0.9514197333488639 1101 32-33 -0.8769185503145209 1101 34-35 -0.886811642778504 1101 36-37 -0.857841974746286 1101 38-39 -0.9925824618424244 1101 40-41 -0.9527082244668392 1101 42-43 -0.9879082477078285 1101 44-45 -0.9768989199088765 1101 46-47 -0.888480500211692 1101 48-49 -0.9457382305445066 1101 50-51 -1.0184012380102914 1101 52-53 -1.1221517199015452 1101 54-55 -1.1073896061979767 1101 56-57 -0.9401877598235089 1101 58-59 -1.1270962452614626 1101 60-61 -1.1862233839516847 1101 62-63 -1.2655265774757112 1101 64-65 -1.3299442102914085 1101 66-67 -1.255466621720359 1101 68-69 -1.4138207908295612 1101 70-71 -1.4209022204105892 1101 72-73 -1.3359271292950723 1101 74-75 -1.5231199951360566 1101 76-77 -1.4881023338775776 1101 78-79 -1.2272796006513147 1101 80-81 -1.1352378958025149 1101 82-83 -1.0866869974175621 1101 84-85 -0.9712523742048127 1101 86-87 -1.1872558694814792 1101 88-89 -1.3158699769414248 1101 90-91 -1.5173730486740382 1101 92-93 -1.4691437945062127 1101 94-95 -1.4169202617187437 1101 96-97 -1.395906692785605 1101 98-99 -1.4404942114441361 1101 100 -1.2833636653545533 1102 1 -0.4607795576446847 1102 2 -0.40248270929180663 1102 3 -0.4633062792475968 1102 4 -0.2581225418756432 1102 5 -0.39594117497681225 1102 6 -0.4084226837739635 1102 7 -0.38481103470765987 1102 8 -0.38091615877284113 1102 9 -0.5957702528224971 1102 10-11 -0.6576137731083236 1102 12-13 -0.6393097573914801 1102 14-15 -0.6563898786079818 1102 16-17 -0.7957720720449757 1102 18-19 -0.767223093292877 1102 20-21 -0.9265784003618904 1102 22-23 -0.8317089574585239 1102 24-25 -0.8927834505682739 1102 26-27 -0.8455214567899318 1102 28-29 -0.8744711326416308 1102 30-31 -0.8370850425967049 1102 32-33 -0.8276645505697715 1102 34-35 -0.7096249802885097 1102 36-37 -0.7240822726823168 1102 38-39 -0.9428203001819568 1102 40-41 -0.8515312147328657 1102 42-43 -0.7671936885208837 1102 44-45 -0.8102281925876369 1102 46-47 -0.7556193629963701 1102 48-49 -0.8354960995129872 1102 50-51 -0.9546501213302889 1102 52-53 -0.9149243348470648 1102 54-55 -0.9245412310398962 1102 56-57 -0.9147048319755484 1102 58-59 -0.893196160567804 1102 60-61 -0.974839628890372 1102 62-63 -0.9771377291933732 1102 64-65 -0.8701441105153265 1102 66-67 -0.9965824895982607 1102 68-69 -1.0979558133951954 1102 70-71 -0.9602679461655796 1102 72-73 -1.0455607422334765 1102 74-75 -1.210994300474077 1102 76-77 -1.0268421387341249 1102 78-79 -0.9385937444913033 1102 80-81 -0.9258328397074713 1102 82-83 -0.888195820639865 1102 84-85 -0.9137691679119619 1102 86-87 -0.9948208689593905 1102 88-89 -1.081225632273064 1102 90-91 -0.9599925189326441 1102 92-93 -0.9615648727840309 1102 94-95 -1.0909854410256479 1102 96-97 -1.1622461867120215 1102 98-99 -1.2655467099068893 1102 100 -1.2344211758252435 1103 1 -0.3899635958646712 1103 2 -0.3944838999944338 1103 3 -0.43128533815677983 1103 4 -0.19968056059934725 1103 5 -0.2537522111549322 1103 6 -0.36403241336157777 1103 7 -0.39858330744417003 1103 8 -0.44452584821340935 1103 9 -0.5200297907446867 1103 10-11 -0.5921200488994316 1103 12-13 -0.5974078082279206 1103 14-15 -0.7517289534584748 1103 16-17 -0.7261558092600353 1103 18-19 -0.7860250687233759 1103 20-21 -0.766865189805074 1103 22-23 -0.7964856774595575 1103 24-25 -0.8413708743103001 1103 26-27 -0.7408647789041254 1103 28-29 -0.8513607549485087 1103 30-31 -0.859055452265487 1103 32-33 -0.7481429834053515 1103 34-35 -0.7163870035331641 1103 36-37 -0.6963671634639894 1103 38-39 -0.6436743184238978 1103 40-41 -0.7724436763024798 1103 42-43 -0.8553088042998311 1103 44-45 -0.8403846828349586 1103 46-47 -0.8208526822047943 1103 48-49 -0.7982414769111301 1103 50-51 -0.9880389995704135 1103 52-53 -1.0105038635746055 1103 54-55 -0.8333291623482992 1103 56-57 -0.8879090656284134 1103 58-59 -0.8059602406141622 1103 60-61 -0.9642463167517406 1103 62-63 -0.9217624681457721 1103 64-65 -0.7468439033908396 1103 66-67 -1.0333039398466468 1103 68-69 -0.9290676564554943 1103 70-71 -0.9818497373260371 1103 72-73 -0.9860353415157768 1103 74-75 -1.0149472789809657 1103 76-77 -0.884320860187799 1103 78-79 -0.65954435799447 1103 80-81 -0.6368171326653602 1103 82-83 -0.772104211445896 1103 84-85 -0.8884054760679554 1103 86-87 -0.821888631542496 1103 88-89 -0.8676662367994474 1103 90-91 -0.8608059372504187 1103 92-93 -0.999501811566887 1103 94-95 -1.0324238754083623 1103 96-97 -0.9905004291409405 1103 98-99 -1.0477528414868758 1103 100 -1.321560216550747 1104 1 -0.40256083849851976 1104 2 -0.3931759431250299 1104 3 -0.4440683359212265 1104 4 -0.32386353011107616 1104 5 -0.32908464970084594 1104 6 -0.44398477318932805 1104 7 -0.38536554354177355 1104 8 -0.4457878973662943 1104 9 -0.6635003739886542 1104 10-11 -0.5892152351862165 1104 12-13 -0.6105348778368906 1104 14-15 -0.8460634101736915 1104 16-17 -0.801205425588357 1104 18-19 -0.8056317941920454 1104 20-21 -0.8574670777333679 1104 22-23 -0.7288839685595718 1104 24-25 -0.7956176390259344 1104 26-27 -0.9739152428139946 1104 28-29 -0.9371288199594972 1104 30-31 -0.8981916625846722 1104 32-33 -0.80705373609009 1104 34-35 -0.8402060157241991 1104 36-37 -0.7657952087830466 1104 38-39 -0.7763254914132673 1104 40-41 -0.708367066354846 1104 42-43 -0.7681268642302896 1104 44-45 -0.9171707083844325 1104 46-47 -0.9840759160883223 1104 48-49 -0.847272983412374 1104 50-51 -0.8063959730174588 1104 52-53 -0.7892578195747078 1104 54-55 -1.0354228964642616 1104 56-57 -0.8617615526878026 1104 58-59 -1.0035370802773897 1104 60-61 -0.8382196626607197 1104 62-63 -0.9943046852477266 1104 64-65 -0.9566549134774434 1104 66-67 -1.0712720407105039 1104 68-69 -0.996116879558862 1104 70-71 -1.113989493632772 1104 72-73 -1.0864145699380572 1104 74-75 -1.1011155318241457 1104 76-77 -0.9322141739590144 1104 78-79 -0.6949025048616342 1104 80-81 -0.8179428418009138 1104 82-83 -0.7662359307158475 1104 84-85 -0.97006775081773 1104 86-87 -0.8759486262011471 1104 88-89 -0.8483199697635833 1104 90-91 -0.8525543497462422 1104 92-93 -0.9156679484065791 1104 94-95 -0.8565857556940202 1104 96-97 -0.7826079249505291 1104 98-99 -0.7075070924057094 1104 100 -0.8981037809084675 1105 1 -0.49609106598886044 1105 2 -0.47883270120222576 1105 3 -0.5541095694729066 1105 4 -0.2837248184717467 1105 5 -0.3276286512167772 1105 6 -0.41279403415794746 1105 7 -0.33794085805706686 1105 8 -0.46890993327111374 1105 9 -0.4668129350290968 1105 10-11 -0.5685054481871532 1105 12-13 -0.7057699909073492 1105 14-15 -0.8884901963348781 1105 16-17 -0.7998028412299263 1105 18-19 -0.8430094202170864 1105 20-21 -0.889028485674686 1105 22-23 -0.9142712993271118 1105 24-25 -0.862329908077136 1105 26-27 -0.8746501446205244 1105 28-29 -0.9423796737481069 1105 30-31 -0.8356497627022392 1105 32-33 -0.9852526500547114 1105 34-35 -0.798551910050115 1105 36-37 -0.7787983483134227 1105 38-39 -0.8792044469894549 1105 40-41 -0.8878366742556878 1105 42-43 -0.7640016548205963 1105 44-45 -0.9870770584088149 1105 46-47 -0.7271606709671303 1105 48-49 -0.8440931382307966 1105 50-51 -0.8179779259575213 1105 52-53 -0.8682652068739003 1105 54-55 -0.8017580486293241 1105 56-57 -0.9521218641621445 1105 58-59 -1.0122075147633183 1105 60-61 -0.8409160292560358 1105 62-63 -1.009067619840124 1105 64-65 -1.0780037320024363 1105 66-67 -1.051373753647077 1105 68-69 -1.0307740936599075 1105 70-71 -0.9565713063682253 1105 72-73 -0.9681991807363026 1105 74-75 -1.0596237620257476 1105 76-77 -0.9480512528607115 1105 78-79 -0.7660507183535259 1105 80-81 -0.8124675649446722 1105 82-83 -0.8769937389877249 1105 84-85 -0.9745578869693183 1105 86-87 -0.9777174891035365 1105 88-89 -1.0530839475165088 1105 90-91 -0.8657430928957126 1105 92-93 -0.7806287844279609 1105 94-95 -0.8561254850369266 1105 96-97 -1.0597543234388134 1105 98-99 -1.08148050042551 1105 100 -1.1729091094490052 1106 1 -0.4560436074291232 1106 2 -0.48895963615279925 1106 3 -0.5081691653026112 1106 4 -0.4011954696239215 1106 5 -0.37737914738350753 1106 6 -0.48291826772862123 1106 7 -0.5255918138571118 1106 8 -0.4840884984695677 1106 9 -0.7674316606755553 1106 10-11 -0.6301227025285527 1106 12-13 -0.6925116731991565 1106 14-15 -0.8041893254139936 1106 16-17 -0.8082789190655575 1106 18-19 -0.6563255035950277 1106 20-21 -0.7630568362081362 1106 22-23 -0.8159004507050582 1106 24-25 -0.7789056149963614 1106 26-27 -0.8173532290780301 1106 28-29 -0.8743330604910113 1106 30-31 -0.8457111567936906 1106 32-33 -0.8918450885833664 1106 34-35 -0.8119507792584741 1106 36-37 -0.8307931827965902 1106 38-39 -0.8997142502073885 1106 40-41 -0.9455047803292089 1106 42-43 -0.8715652942471834 1106 44-45 -0.8123675564296278 1106 46-47 -0.9435529133217813 1106 48-49 -1.1035073474890567 1106 50-51 -0.988416307083348 1106 52-53 -1.0083837137185583 1106 54-55 -1.0864259234408067 1106 56-57 -0.9974564514939672 1106 58-59 -1.1150396274916048 1106 60-61 -1.1246592297034113 1106 62-63 -1.1458577475426281 1106 64-65 -1.0819041607343536 1106 66-67 -1.1178419037800111 1106 68-69 -1.1126929406192474 1106 70-71 -1.2284806436728886 1106 72-73 -1.4251274974775825 1106 74-75 -1.316763603790676 1106 76-77 -1.2013615646557732 1106 78-79 -1.117399065776901 1106 80-81 -1.0125259287059123 1106 82-83 -1.0200554789754257 1106 84-85 -1.0179264977323754 1106 86-87 -1.2835878318398635 1106 88-89 -1.0843435730514308 1106 90-91 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16-17 0.85972334073886 2310 18-19 0.8868875145239485 2310 20-21 1.0297478954021457 2310 22-23 1.0782820007433926 2310 24-25 1.1341322543012353 2310 26-27 1.1920702611855063 2310 28-29 1.2031771726326923 2310 30-31 0.9907401414855102 2310 32-33 1.1807707987842733 2310 34-35 1.200568359483647 2310 36-37 1.0766468478134144 2310 38-39 1.1584555438600503 2310 40-41 1.1711507830212966 2310 42-43 1.3063252074849174 2310 44-45 1.2484325852774418 2310 46-47 0.986241477626379 2310 48-49 1.1057120294751144 2310 50-51 1.247366555587206 2310 52-53 1.281961713242886 2310 54-55 1.2937645523502752 2310 56-57 1.3338696978340536 2310 58-59 1.3096953157826334 2310 60-61 1.3202619317593758 2310 62-63 0.7342716200695918 2310 64-65 0.8147839756718795 2310 66-67 1.1621157818016385 2310 68-69 1.3226026841749317 2310 70-71 1.338988377376083 2310 72-73 1.3376560439730412 2310 74-75 1.2932045081243615 2310 76-77 1.2560928643192497 2310 78-79 1.1541392707186802 2310 80-81 1.188432411292407 2310 82-83 1.0970272425272434 2310 84-85 1.1546457951403575 2310 86-87 1.1630334250595205 2310 88-89 1.1843863833161343 2310 90-91 1.2009800221983902 2310 92-93 1.278631030189743 2310 94-95 1.4041514357300926 2310 96-97 1.3900305984962564 2310 98-99 1.3851206770015345 2310 100 1.5197768373654021 2311 1 0.40078617292602203 2311 2 0.3978811803296338 2311 3 0.515842255520333 2311 4 0.3698485425608382 2311 5 0.40344322826225465 2311 6 0.49781761960193194 2311 7 0.5595493844562327 2311 8 0.5518239327933685 2311 9 0.6725169374361641 2311 10-11 0.6765431729200913 2311 12-13 0.7752448280892921 2311 14-15 0.8744728203965764 2311 16-17 0.750704338488184 2311 18-19 0.8531489912190864 2311 20-21 0.8146612364393206 2311 22-23 0.9561349713074421 2311 24-25 0.9739956314448648 2311 26-27 0.4793600103186293 2311 28-29 0.8065912185797686 2311 30-31 1.004356778822988 2311 32-33 1.0705945639900705 2311 34-35 1.0736141981577063 2311 36-37 1.136912503196264 2311 38-39 1.172727898795337 2311 40-41 1.1740963502940645 2311 42-43 1.0726654303857543 2311 44-45 1.1529163885288796 2311 46-47 0.7472997878010688 2311 48-49 0.9903259947113767 2311 50-51 1.153276858566329 2311 52-53 1.1376792263112065 2311 54-55 1.2140483299153928 2311 56-57 1.3642475047325817 2311 58-59 1.3511286400273974 2311 60-61 1.2846733233252507 2311 62-63 1.3021636617258139 2311 64-65 1.3797067591657353 2311 66-67 1.4641934227406708 2311 68-69 1.449174170220843 2311 70-71 1.4900721912749404 2311 72-73 1.4339954509827493 2311 74-75 1.3910801633893257 2311 76-77 1.3420040039912564 2311 78-79 0.9912655218961959 2311 80-81 0.9502759107395811 2311 82-83 1.1507306370580572 2311 84-85 1.2288264389824555 2311 86-87 1.238146449863759 2311 88-89 1.2688305754001696 2311 90-91 1.2942815637703546 2311 92-93 1.416416011367616 2311 94-95 1.3605048045707377 2311 96-97 1.3541780388824662 2311 98-99 1.5370208057644064 2311 100 1.4542693318989706 2312 1 0.2894191023140813 2312 2 0.3730957700460209 2312 3 0.48910571804462677 2312 4 0.21738843538519603 2312 5 0.3955568326541936 2312 6 0.4432192898252225 2312 7 0.33108964685177256 2312 8 0.5023996740220156 2312 9 0.692978439444957 2312 10-11 0.6508932194145274 2312 12-13 0.7439670357406172 2312 14-15 0.9493648176903662 2312 16-17 0.9421107281950398 2312 18-19 0.8969932095164381 2312 20-21 0.9290640809452242 2312 22-23 1.0009154117528354 2312 24-25 0.9953007020210407 2312 26-27 1.0386124781003687 2312 28-29 1.024853846588492 2312 30-31 1.0809833018138875 2312 32-33 1.0158781353590314 2312 34-35 1.1458292259947598 2312 36-37 1.2853145549123823 2312 38-39 1.0228598938240054 2312 40-41 1.085405787174885 2312 42-43 1.2011543760349497 2312 44-45 0.9337986428534535 2312 46-47 1.0229050723708752 2312 48-49 1.2173987972530682 2312 50-51 1.0278320319667387 2312 52-53 1.2371695757347965 2312 54-55 1.1086600790004084 2312 56-57 1.2253325537901745 2312 58-59 1.2534174852366817 2312 60-61 1.2710361095938936 2312 62-63 1.2924620084908724 2312 64-65 1.2925695499049965 2312 66-67 1.3465399910934508 2312 68-69 1.2720309554284555 2312 70-71 1.1777291845153641 2312 72-73 1.2547364784502335 2312 74-75 1.3388729069784588 2312 76-77 1.3192041819218971 2312 78-79 1.2955113433575534 2312 80-81 1.203832399992212 2312 82-83 1.2266440590487413 2312 84-85 1.2170576459900069 2312 86-87 1.217001129409791 2312 88-89 1.26120865416652 2312 90-91 1.3200519632052732 2312 92-93 1.4210060332438488 2312 94-95 1.4699132597036062 2312 96-97 1.182632598019815 2312 98-99 0.89951529200086 2312 100 1.0879166268763782 2313 1 0.1516203201241204 2313 2 0.314291364062818 2313 3 0.4086991416378041 2313 4 0.23897446720877014 2313 5 0.42488561228524446 2313 6 0.36550015380736056 2313 7 0.32972629863021297 2313 8 0.5082890998949523 2313 9 0.7140733344132073 2313 10-11 0.49371037925354244 2313 12-13 0.5566438333771089 2313 14-15 0.5683241106161319 2313 16-17 0.8676212749882524 2313 18-19 0.7417383504892854 2313 20-21 0.6840754797402155 2313 22-23 0.7327986700634881 2313 24-25 0.6180861516918696 2313 26-27 0.8168194069393238 2313 28-29 0.7340016703491656 2313 30-31 0.873940983787513 2313 32-33 1.0243703830153024 2313 34-35 1.0759032890836053 2313 36-37 1.0579193540377005 2313 38-39 1.1473797341245628 2313 40-41 1.0758531171816301 2313 42-43 1.1307006632845784 2313 44-45 0.9785714084570145 2313 46-47 1.0377783916301908 2313 48-49 1.1121918451512656 2313 50-51 0.9837852852758857 2313 52-53 1.1256312036688243 2313 54-55 1.083299286784161 2313 56-57 0.8925697417772369 2313 58-59 0.9252445056149341 2313 60-61 0.7192532304676433 2313 62-63 0.9895926090212583 2313 64-65 1.0800569007319893 2313 66-67 1.0058314629337772 2313 68-69 1.163210847023592 2313 70-71 1.1808610906730408 2313 72-73 1.2380573806997575 2313 74-75 1.315556337434181 2313 76-77 1.2143088792722594 2313 78-79 1.1003364939844857 2313 80-81 1.004753245247752 2313 82-83 1.0522894576576185 2313 84-85 1.0435618071317485 2313 86-87 1.0757670789991955 2313 88-89 1.130469200959734 2313 90-91 1.0421169915859423 2313 92-93 1.232349070092198 2313 94-95 1.4184332337655334 2313 96-97 1.4341216383110655 2313 98-99 1.4264176113066966 2313 100 1.4213219296423318 2314 1 -0.37074587135466075 2314 2 0.10588221607559234 2314 3 0.35620562124701394 2314 4 0.2649589161466679 2314 5 0.3827237307516569 2314 6 0.4381660799472584 2314 7 0.4531778680804095 2314 8 0.5035620376122836 2314 9 0.5007107969277627 2314 10-11 0.6366193887642595 2314 12-13 0.4976071733315095 2314 14-15 0.8922018303892898 2314 16-17 0.8932788895589638 2314 18-19 0.8523915129654256 2314 20-21 0.900344286985856 2314 22-23 1.0024874006556814 2314 24-25 0.8736875021109256 2314 26-27 0.7990733182805982 2314 28-29 0.8763252567222821 2314 30-31 0.9217578195543581 2314 32-33 1.0198157959681637 2314 34-35 1.038690330063723 2314 36-37 1.0930487129460076 2314 38-39 0.7804767639769281 2314 40-41 0.8236876223539014 2314 42-43 1.0217233644998984 2314 44-45 1.0266522926420052 2314 46-47 0.9299143646535697 2314 48-49 0.9343028725477822 2314 50-51 0.9780424803471135 2314 52-53 0.9953697163218038 2314 54-55 0.971998163387191 2314 56-57 1.0437238120439005 2314 58-59 0.9798124439824605 2314 60-61 1.0246736183805751 2314 62-63 1.0358579121657243 2314 64-65 1.00245867918742 2314 66-67 0.738655162966829 2314 68-69 0.7611936560818435 2314 70-71 0.8869414507318467 2314 72-73 1.0491252778717524 2314 74-75 1.1122050567497226 2314 76-77 0.940082685073584 2314 78-79 0.8289937382918069 2314 80-81 0.9359321519483323 2314 82-83 0.9074633672915233 2314 84-85 0.8944292955250326 2314 86-87 0.911309336176398 2314 88-89 0.9011572952548015 2314 90-91 1.047698736906277 2314 92-93 0.8965122401026484 2314 94-95 1.0357613119397442 2314 96-97 1.0590532686313665 2314 98-99 1.1443660047308981 2314 100 1.2465620796933052 2315 1 0.4060175873377254 2315 2 0.513155363354926 2315 3 0.43187432144566174 2315 4 0.34304180867860623 2315 5 0.44163825468579887 2315 6 0.4470808611002113 2315 7 0.45235328799716257 2315 8 0.28587705341926295 2315 9 0.5456729269193801 2315 10-11 0.6173135314268308 2315 12-13 0.4033324623732213 2315 14-15 0.6401878599547999 2315 16-17 0.8199139185468169 2315 18-19 0.7607938508236742 2315 20-21 0.555774426251169 2315 22-23 0.9229860697068943 2315 24-25 0.9219857953126009 2315 26-27 0.773814219658469 2315 28-29 0.8092947041310055 2315 30-31 0.9222449849347072 2315 32-33 0.8109792445691184 2315 34-35 0.9231718470599262 2315 36-37 0.9676887211262724 2315 38-39 0.9731063809400595 2315 40-41 0.9837942462456652 2315 42-43 1.0777630630168957 2315 44-45 1.0396866973139964 2315 46-47 1.0260008826317915 2315 48-49 0.9861651261665187 2315 50-51 1.032329708347902 2315 52-53 1.0652790054655554 2315 54-55 0.9959851211954813 2315 56-57 0.8653505300453261 2315 58-59 0.9371930840598424 2315 60-61 1.1088006596672955 2315 62-63 1.1302152150270572 2315 64-65 1.2548060474831146 2315 66-67 1.1869664791921295 2315 68-69 1.2374870667771987 2315 70-71 1.1892323260249 2315 72-73 1.2521392058122913 2315 74-75 0.6124027628794053 2315 76-77 0.6748621143057463 2315 78-79 0.9570322588171294 2315 80-81 1.0244423427109588 2315 82-83 0.9218198766504422 2315 84-85 0.9338070289593823 2315 86-87 0.9989943478530812 2315 88-89 0.9608527777807261 2315 90-91 1.01476771728316 2315 92-93 1.1199733017200835 2315 94-95 1.159825061895436 2315 96-97 1.25228505949514 2315 98-99 1.2557665202819592 2315 100 1.24166237846881 2316 1 0.2961671702040327 2316 2 0.13500485674801155 2316 3 0.2586104846873809 2316 4 0.08509920313862551 2316 5 0.28292913406335884 2316 6 0.43341903760966005 2316 7 0.4708397741165058 2316 8 0.4710602994703308 2316 9 0.7163516201377433 2316 10-11 0.6364832360746391 2316 12-13 0.4037516691024834 2316 14-15 0.5134682810265545 2316 16-17 0.7736588602958321 2316 18-19 0.7601553476796568 2316 20-21 0.7732729570819288 2316 22-23 0.7791623255830444 2316 24-25 0.47914581794763933 2316 26-27 0.7310852029540129 2316 28-29 0.9759786932923333 2316 30-31 0.5425774806092747 2316 32-33 0.692402853641191 2316 34-35 0.7474566237780422 2316 36-37 0.7854785528761496 2316 38-39 0.7143112468795181 2316 40-41 0.7408846062738448 2316 42-43 0.6901857467918191 2316 44-45 0.599670652132211 2316 46-47 0.7869464941493405 2316 48-49 0.8571853685097537 2316 50-51 0.8501400936091557 2316 52-53 0.8602010216935625 2316 54-55 0.7937168130402554 2316 56-57 0.8592966188571651 2316 58-59 0.7264890629722984 2316 60-61 0.8365422327630156 2316 62-63 0.8983106184128928 2316 64-65 0.9342303836046923 2316 66-67 0.932873783351134 2316 68-69 1.0009952518858753 2316 70-71 0.9314185602239959 2316 72-73 0.9429968129250881 2316 74-75 0.9614744729131601 2316 76-77 0.8103279729069257 2316 78-79 0.5951435772007159 2316 80-81 0.6005432574094378 2316 82-83 0.5195891096075478 2316 84-85 0.640204859470817 2316 86-87 0.7205440783650587 2316 88-89 0.6775392503439477 2316 90-91 0.48385042503741715 2316 92-93 0.3826208925584673 2316 94-95 0.4909914089272931 2316 96-97 0.7322653380295314 2316 98-99 0.7884178072147812 2316 100 0.3796023350467763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 13.0 13 89.0 14 404.0 15 1230.0 16 2861.0 17 6204.0 18 9831.0 19 13463.0 20 17873.0 21 22716.0 22 27561.0 23 28591.0 24 28478.0 25 29746.0 26 34486.0 27 41646.0 28 51174.0 29 62944.0 30 77522.0 31 96528.0 32 121678.0 33 158969.0 34 220656.0 35 307053.0 36 456758.0 37 667377.0 38 543918.0 39 73788.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.146165641650335 19.66738733174587 13.389058892208727 23.797388134395064 2 15.928856238370267 20.587161579791047 40.27481211183876 23.209170069999917 3 18.75897956389003 26.303311503669157 30.32393288385169 24.613776048589127 4 12.448425538580645 16.84216192277779 38.424968082737024 32.28444445590454 5 12.7561602345682 37.10353382792425 34.4538153580951 15.686490579412451 6 29.616329555432042 36.881435119177084 18.94041548858977 14.561819836801106 7 26.666084537411848 32.47018171776892 22.8026043732514 18.06112937156784 8 26.001433830340815 35.14128061993024 20.523815277542834 18.33347027218611 9 26.38829631596518 16.29425875249901 21.28747871571151 36.029966215824295 10-11 25.30322426399868 26.055118620191454 28.12709336146446 20.51456375434541 12-13 25.446364273458833 24.093650064844574 28.370910965580016 22.089074696116576 14-15 22.727738466431088 25.350217539730846 26.677414500908792 25.24462949292927 16-17 21.751187383168762 27.605222826562393 27.69500657066213 22.948583219606718 18-19 21.353229743118824 26.946118589046502 29.958354478303246 21.742297189531428 20-21 22.446533433218143 26.365626736908244 29.62346648622959 21.56437334364402 22-23 22.305760292565832 26.276616482604737 29.44791087697252 21.969712347856905 24-25 21.765030556834553 26.293941056740948 29.972747434318194 21.968280952106305 26-27 21.742966142107772 26.40881761589916 29.537031666859992 22.311184575133073 28-29 21.583150251250856 26.780742766841364 29.67556893727114 21.96053804463664 30-31 22.34368162974391 26.45625624696714 29.693852694339885 21.506209428949067 32-33 22.301492427168384 26.5937170238982 29.309952839088787 21.794837709844625 34-35 22.406576216986412 26.587036398118897 29.47098813587209 21.535399249022603 36-37 22.262657855559162 26.522586386445685 29.25553705135598 21.959218706639174 38-39 22.184420399384056 26.83598903985268 29.225995994686222 21.75359456607704 40-41 22.23290001541791 26.195979518184675 29.648012499301203 21.923107967096215 42-43 22.002910515894015 26.5229259110506 29.208330332759964 22.26583324029542 44-45 21.9309840791486 27.024618896593417 29.08837448513257 21.956022539125414 46-47 21.99972994129285 26.611014592515893 28.62894610395198 22.760309362239283 48-49 22.116919031826445 26.671881255593533 28.77790926131938 22.433290451260646 50-51 22.124528172380643 26.97912220687797 28.43686351507488 22.459486105666507 52-53 22.019779946404473 27.283529751447194 28.106942283623205 22.58974801852513 54-55 21.946034170517457 27.0869182730419 28.380573977043493 22.58647357939715 56-57 22.324191094650452 26.975895273865202 28.04597234499784 22.65394128648651 58-59 21.974291647580866 27.152552089131916 28.462943040988005 22.41021322229922 60-61 22.266620996900535 27.441235352136772 28.089612845620614 22.202530805342075 62-63 22.437689954914276 27.13894570139027 27.914884087729064 22.508480255966386 64-65 22.021755775569048 26.93154345715928 28.092177032841235 22.95452373443043 66-67 21.982639108303747 26.9698976426999 28.0074146818309 23.04004856716545 68-69 21.631575002236637 26.9945829098503 28.196353196534545 23.177488891378516 70-71 21.759562084033828 26.694788625919376 28.30496153244798 23.240687757598813 72-73 21.880304768093993 26.99718776775681 28.126899884661082 22.995607579488112 74-75 22.100824423101255 27.144350191661648 27.788578303272633 22.966247081964468 76-77 21.893800751756107 26.95603561139972 27.89139567369582 23.25876796314835 78-79 21.684201740574146 27.08416448767271 27.928542103190534 23.30309166856261 80-81 21.651709296714962 27.1859496043634 27.884377288211777 23.27796381070986 82-83 22.063962820993837 26.924883219292443 28.31382583010035 22.69732812961337 84-85 22.17166657667017 27.213925977744807 27.674294340133578 22.940113105451452 86-87 21.593894149613195 27.36161959525593 27.943086170277244 23.101400084853626 88-89 21.737075971500733 26.859386350673063 27.973813871290282 23.429723806535925 90-91 21.945710218851865 26.835146339926286 28.005088850996696 23.214054590225157 92-93 21.982708742361098 26.99356009924003 27.894820648812185 23.12891050958668 94-95 21.353603484937324 26.888919072945573 28.186989264411856 23.57048817770525 96-97 21.40170744635432 26.975139078417186 27.990262496918767 23.632890978309728 98-99 21.30222777263022 27.059533497159894 27.81467187688837 23.823566853321516 100 21.439417307185902 26.772817681908588 27.908170056930388 23.87959495397512 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1413.0 1 1019.5 2 1730.0 3 2598.5 4 1644.0 5 969.5 6 1295.0 7 2377.0 8 3644.5 9 3999.0 10 3345.5 11 2739.5 12 2763.5 13 3102.5 14 3534.5 15 3925.5 16 4351.5 17 4869.5 18 5393.0 19 5785.0 20 6261.5 21 6886.0 22 7733.0 23 9076.0 24 10683.0 25 12702.5 26 15559.0 27 18848.0 28 22606.0 29 27639.0 30 33960.0 31 40508.0 32 48398.5 33 56189.5 34 63082.0 35 69959.5 36 76893.5 37 86138.5 38 91041.0 39 90938.5 40 90395.0 41 89769.5 42 90731.5 43 94221.5 44 99961.0 45 108654.5 46 115970.0 47 120080.5 48 123545.5 49 125385.0 50 124878.5 51 123659.0 52 120340.0 53 115024.0 54 110336.5 55 101303.0 56 91295.5 57 80749.5 58 70019.5 59 61784.5 60 49242.0 61 36080.0 62 27638.0 63 21279.5 64 15364.0 65 10379.0 66 7388.5 67 5462.5 68 4231.5 69 3299.5 70 2545.5 71 1931.5 72 1505.0 73 1231.5 74 964.0 75 713.5 76 495.5 77 312.5 78 189.0 79 110.0 80 67.5 81 48.0 82 35.0 83 24.5 84 14.0 85 7.0 86 6.0 87 4.5 88 2.5 89 1.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043433782009456835 2 0.0 3 0.0 4 0.03292976647897989 5 0.0 6 0.0 7 8.377435699153397E-4 8 0.0 9 0.011180654567716263 10-11 4.5109269149287516E-4 12-13 0.0 14-15 1.2888362614082148E-4 16-17 2.0943589247883492E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.5109269149287516E-4 26-27 4.833135980280805E-4 28-29 1.1277317287321879E-4 30-31 0.0022232425509291706 32-33 0.015321041057490152 34-35 0.026372811999065594 36-37 0.031447604778360436 38-39 0.02245797185503814 40-41 0.001337167621211023 42-43 4.833135980280805E-5 44-45 0.011245096380786674 46-47 0.01759261496822213 48-49 0.0033026429198585506 50-51 0.011261206834054276 52-53 0.010665120063152978 54-55 0.010149585558589691 56-57 4.3498223822527245E-4 58-59 0.008522429778561819 60-61 0.00438204328878793 62-63 0.001369388527746228 64-65 0.03507245676357104 66-67 0.06092973425807335 68-69 0.05856149762773576 70-71 0.05187565952168064 72-73 0.04552814093424518 74-75 0.03552354945506392 76-77 0.03135094205875483 78-79 0.014853837912729675 80-81 0.0010471794623941745 82-83 0.03798844880500713 84-85 0.051634002722666605 86-87 0.056805458221567066 88-89 0.06966159992911401 90-91 0.04831524934954045 92-93 0.03860064602917603 94-95 0.012614484908532902 96-97 0.0035442997188725902 98-99 0.022554634574643757 100 0.032671999226698244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3103575.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.37652194875383 #Duplication Level Percentage of deduplicated Percentage of total 1 83.07674431659505 59.297290641444576 2 11.330052215148449 16.173994412301404 3 2.763941116832527 5.918415113719803 4 1.0336629535155117 2.951170659568546 5 0.5123024585851148 1.8283183839800499 6 0.3036187527105828 1.3002750340140108 7 0.21249279688902545 1.061689774677113 8 0.1516383439708577 0.8658734069366887 9 0.09987997083754445 0.6416176437654195 >10 0.48225415962403023 5.867242732433636 >50 0.022947448985571724 1.1142793237837634 >100 0.00956453929354237 1.2353444041798538 >500 5.005150068416413E-4 0.24846751666044656 >1k 2.502575033300247E-4 0.4534934084314551 >5k 5.005150066600494E-5 0.24325166362137843 >10k+ 1.0010300133200987E-4 0.7992758804818769 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12097 0.38977630635637933 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10276 0.33110203555577034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6809 0.21939215259821335 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3921 0.12633817452454024 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 3.222090653520537E-5 20-21 0.0 0.0 0.0 8.055226633801342E-5 3.222090653520537E-5 22-23 0.0 0.0 0.0 4.188717849576698E-4 4.833135980280805E-5 24-25 0.0 0.0 0.0 8.538540231829422E-4 6.444181307041074E-5 26-27 0.0 0.0 0.0 0.0016754871398306792 6.444181307041074E-5 28-29 0.0 0.0 0.0 0.004527037368196354 6.444181307041074E-5 30-31 0.0 0.0 0.0 0.014660512473518444 6.444181307041074E-5 32-33 0.0 0.0 0.0 0.04042112724841514 6.444181307041074E-5 34-35 0.0 0.0 0.0 0.07837735514688707 9.66627196056161E-5 36-37 0.0 0.0 0.0 0.1404509315869602 9.66627196056161E-5 38-39 0.0 0.0 0.0 0.23245773019823912 1.1277317287321879E-4 40-41 0.0 0.0 0.0 0.33962446533433216 1.7721498594362953E-4 42-43 0.0 0.0 0.0 0.44197417494341207 1.933254392112322E-4 44-45 0.0 0.0 0.0 0.5633019985017278 1.933254392112322E-4 46-47 0.0 0.0 0.0 0.7009819321266604 1.933254392112322E-4 48-49 0.0 0.0 0.0 0.8506802638892246 1.933254392112322E-4 50-51 0.0 0.0 0.0 1.005469498884351 1.933254392112322E-4 52-53 0.0 0.0 0.0 1.1546361856890845 2.094358924788349E-4 54-55 0.0 0.0 0.0 1.3295312663621792 2.2554634574643758E-4 56-57 0.0 0.0 0.0 1.5341179124074655 2.2554634574643758E-4 58-59 0.0 0.0 0.0 1.7562810629677066 2.2554634574643758E-4 60-61 0.0 0.0 0.0 1.9773164817992155 2.2554634574643758E-4 62-63 0.0 0.0 0.0 2.2038616756482443 2.2554634574643758E-4 64-65 0.0 0.0 0.0 2.435981086327864 2.2554634574643758E-4 66-67 0.0 0.0 0.0 2.696664330650943 2.2554634574643758E-4 68-69 0.0 0.0 0.0 2.990792875957565 2.2554634574643758E-4 70-71 0.0 0.0 0.0 3.289384016819313 2.4165679901404027E-4 72-73 0.0 0.0 0.0 3.581740412266499 2.5776725228164295E-4 74-75 0.0 0.0 0.0 3.8798321290769513 2.5776725228164295E-4 76-77 0.0 0.0 0.0 4.215106772029031 2.5776725228164295E-4 78-79 0.0 0.0 0.0 4.581845130212738 2.899881588168483E-4 80-81 0.0 0.0 0.0 4.952884979418895 3.0609861208445103E-4 82-83 0.0 0.0 0.0 5.341259676340994 3.222090653520537E-4 84-85 0.0 0.0 0.0 5.743231595820948 3.222090653520537E-4 86-87 0.0 0.0 0.0 6.155723641284648 3.222090653520537E-4 88 0.0 0.0 0.0 6.48916169256422 3.222090653520537E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5715 0.0 51.409695 1 TACCTGG 3120 0.0 50.00308 2 GTACCTG 3590 0.0 43.735416 1 ACCTGGG 3765 0.0 40.566418 3 GTATCAA 10085 0.0 39.15477 1 CCTGGGG 3390 0.0 32.438774 4 ATCAACG 12305 0.0 31.20245 3 TATCAAC 12735 0.0 30.773746 2 CAACGCA 12515 0.0 30.70851 5 ATAACGC 405 0.0 30.169394 3 TCAACGC 13235 0.0 29.116432 4 TAACGCA 420 0.0 29.091915 4 AACGCAG 13620 0.0 28.838022 6 GTACATG 10305 0.0 26.686346 1 GTGGTAT 2405 0.0 26.583067 1 TACATGG 10375 0.0 26.04312 2 ACGCAGA 14740 0.0 26.00932 7 TGGTATC 2565 0.0 25.831224 2 CTGGGGG 2420 0.0 25.82097 5 CGCAGAG 14970 0.0 25.54653 8 >>END_MODULE