FastQCFastQC Report
Mon 6 Jun 2016
ERR1211180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5767849
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162740.28215024353099394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132920.23044986094469533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82600.14320763251603846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84600.047.6137541
GTATCAA149500.039.2513161
CAACGCA173300.032.5220955
ATCAACG175950.031.9788443
TATCAAC187250.030.9104062
GTACTAG32800.029.5544171
AACGCAG193400.029.5312256
TCAACGC192300.029.5038244
TACCTGG43200.028.9747282
GTACATG184150.026.8571551
GTGGTAT35350.026.6237741
ACGCAGA213900.026.3713347
GTACCTG50300.026.1014861
TACATGG188300.025.9898932
CGCAGAG218250.025.672978
CTAACGC14700.025.5817663
GCATCGT33100.024.9941738
ACATGGG188700.024.6865063
TAACGCC15850.024.021834
CTGTGCG47600.023.9970159
Produced by FastQC (version 0.11.5)