FastQCFastQC Report
Mon 6 Jun 2016
ERR1211180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5767849
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145390.25206970570831516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126870.21996068204975547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82430.14291289525783354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90500.045.5075761
GTATCAA152750.037.672841
CAACGCA177200.031.1831115
ATCAACG177400.031.1309153
TACCTGG40400.030.5940382
GTACTAG31250.030.389921
TATCAAC194050.029.3529282
TCAACGC195150.028.5161444
AACGCAG199550.028.396936
GTACATG185000.026.5566181
GTACCTG48450.026.1027741
ACGCAGA217450.025.5623747
TACATGG189200.025.4107192
GTGGTAT39850.024.7753141
CGCAGAG224450.024.7230688
ACATGGG187850.024.3950123
TCTAACG14100.023.6647382
CTAACGC13900.023.3314593
CTAGTAC49750.022.5794033
TAGTACT48550.022.5566354
Produced by FastQC (version 0.11.5)