FastQCFastQC Report
Mon 6 Jun 2016
ERR1211179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7858868
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236640.30111206855745637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182610.23236170909092757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117020.14890185202245412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130600.045.2249221
GTATCAA228850.039.484591
GTACTAG57500.037.48651
CAACGCA259900.033.3167535
GCATCGT58250.033.248528
ATCAACG266850.032.5371133
TAGGCAT74800.031.360165
TATCAAC286500.031.1285652
AACGCAG291400.030.4577146
TCAACGC301000.029.0640724
CTAGGCA82050.028.8181384
GTACATG310500.028.7378331
TACATGG314650.027.8799972
TAGTACT76250.027.8043824
CTGTGCG74900.027.677569
ACGCAGA313200.027.5876147
ACATGGG310750.027.0934163
CGCAGAG320750.026.8637628
GGGCACC72100.025.42212794
CTAGTAC85650.025.4116553
Produced by FastQC (version 0.11.5)