FastQCFastQC Report
Mon 6 Jun 2016
ERR1211179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7858868
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206260.26245510167622105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178080.22659752015175721No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118920.15131950301239314No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141750.045.2106481
GTATCAA238050.037.764841
GTACTAG53100.033.1165471
CAACGCA283350.030.6765735
ATCAACG286100.030.2752293
TATCAAC306250.029.140412
AACGCAG315450.028.3443556
TCAACGC317350.027.6345924
GTACATG309700.026.9023671
TACATGG312950.026.2790642
TAGGCAT71150.026.1504175
TACCTGG51100.025.9343282
ACATGGG308800.025.5536753
ACGCAGA344250.025.2223997
GTGGTAT59150.025.039391
CGCAGAG350950.024.7140988
CTAGGCA79350.024.520734
CTAACGC23250.024.2570653
CATGGGG240600.023.9287724
TAACGCC24200.023.6932374
Produced by FastQC (version 0.11.5)