FastQCFastQC Report
Mon 6 Jun 2016
ERR1211178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6787622
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291980.43016538045283015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233810.3444652633867944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144920.2135062913049666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138650.047.8582571
GTATCAA246750.040.4902531
GTACTAG58000.037.405081
GCATCGT53250.036.7219168
TACCTGG38150.035.4034042
CAACGCA285800.033.141155
ATCAACG287500.032.945193
TAGGCAT71800.032.0139475
TATCAAC315400.031.2742732
GTGGTAT49850.030.8702551
ACCTGGG41850.030.5511993
AACGCAG325150.029.8391976
GTACATG327900.029.7376021
CTGTGCG75650.029.700319
CTAGGCA80950.029.150214
TACATGG333550.028.8529362
TCAACGC331500.028.7567124
TAGTACT81500.028.6074314
ACATGGG328000.028.3183443
GGGCACC70850.027.79366194
Produced by FastQC (version 0.11.5)